                         SEQUENCE LISTING

<110>  Conagen Inc.
       Chen, Hui
       Yu, Xiaoden
 
<120>  BIOSYNTHETIC PRODUCTION OF GAMMA- AND DELTA-LACTONES USING 
       CYTOCHROME P450 ENZYMES HAVING SUBTERMINAL HYDROXYLASE ACTIVITY

<130>  CCN-1704PCT

<150>  US 62/821,894
<151>  2019-03-21

<160>  8     

<170>  PatentIn version 3.5

<210>  1
<211>  3243
<212>  DNA
<213>  Myceliophthora thermophila

<400>  1
atggcggata agaccaccga aaccgtgccg attccgggtc cgccgggcct gccgctggtt       60

ggtaatgcgc tggcgtttga tagcgaactg ccgctgcgta ccttccagga atttgcggag      120

gaatacggcg agatctatcg tctgaccctg ccgaccggta ccaccctggt ggttagcagc      180

caagcgctgg ttcacgaact gtgcgacgat aagcgtttca agaagccggt tgctgcggcg      240

ctggcggaag tgcgtaacgg cgttaacgac ggtctgttta ccgcgcgtga agaggagccg      300

aactggggca tcgcgcaccg tattctgatg ccggcgtttg gtccggcgag cattcagggc      360

atgtttaccg aaatgcacga gatcgcgagc caactggcgc tgaaatgggc gcgtcacggt      420

ccggacaccc cgattttcgt taccgacgat tttacccgtc tgaccctgga taccctggcg      480

ctgtgcacca tgaacttccg ttttaacagc tactatcacg acgaactgca cccgttcatc      540

aacgcgatgg gcaactttct gaccgagagc ggtgcgcgtg cgatgcgtcc ggcgatcacc      600

agcattttcc accaggcggc gaaccgtaag tactgggaag atattgaggt tctgcgtaaa      660

accgcgcaag gtgtgctgga cacccgtcgt aagcacccga ccaaccgtaa agatctgctg      720

agcgcgatgc tggacggcgt ggatgcgaaa accggtcaga aactgagcga cagcagcatc      780

attgataacc tgatcacctt tctgattgcg ggccacgaaa ccaccagcgg tctgctgagc      840

ttcgcgtttt acctgctgat taagcaccag gacgcgtatc gtaaagcgca agaagaggtg      900

gatcgtgtta tcggcaaggg cccgattaaa gttgaacaca tcaagaaact gccgtacatc      960

gcggcggtgc tgcgtgaaac cctgcgtctg tgcccgacca ttccgatcat taaccgtgcg     1020

gcgaagcagg acgaagttat cggtggcaag tacgcggtgg cgaaagatca gcgtctggcg     1080

ctgctgctgg cgcaaagcca cctggacccg gcggtttatg gcgaaaccgc gaagcaattc     1140

attccggagc gtatgctgga cgaaaacttt gagcgtctga accgtgagta tccggattgc     1200

tggaaaccgt tcggtaccgg catgcgtgcg tgcatcggtc gtccgtttgc gtggcaggaa     1260

gcggtgctgg ttatggcgat gctgctgcaa aacttcgact ttgttctgca cgatccgtac     1320

tatgagctgc actacaagca gaccctgacc accaagccga aagacttcta tatgcgtgcg     1380

atcctgcgtg atggcctgac cgcgaccgaa ctggagcacc gtctggcggg taacgcggcg     1440

agcgtggcgc gtagcggtgg cggtggcggt ggcccgagca aaccgaccgc gcagaaaacc     1500

agcccggcgg aagcgaaacc gatgagcatc ttctacggca gcaacaccgg tacctgcgag     1560

agcctggcgc aacgtctggc gaccgatgcg gcgagccacg gttatgctgc ggcggcggtg     1620

gaaccgctgg acaccgcgac cgagaagctg ccgaccgatc gtccggtggt tatcattacc     1680

gcgagcttcg agggtcagcc gccggacaac gcggcgaagt tttgcggctg gctgaaaaac     1740

ctggaaggtg atgagctgaa aaacgtgagc tacgcggttt tcggttgcgg ccaccacgac     1800

tggagccaga cctttcaccg tattccgaag ctggttcacc aaaccatgaa agcgcacggt     1860

gcgagcccga tctgcgacga aggcctgacc gatgtggcgg agggtaacat gttcaccgat     1920

tttgaacaat gggaggacga tgtgttctgg ccggcggttc gtgcgcgtta tggcgcggcg     1980

ggtgcggttg cggaaaccga ggacgcgccg ggtagcgatg gtctgaacat ccactttagc     2040

agcccgcgta gcagcaccct gcgtcaggac gtgcgtgaag cgaccgtggt tggtgaagcg     2100

ctgctgaccg cgccggatgc gccgccgaag aaacacattg aagttcaact gccggacggc     2160

gcgacctaca aagtgggtga ttatctggcg gtgctgccgg ttaacagcaa ggagagcatt     2220

ggtcgtgtta tgcgtaaatt ccagctgagc tgggacagcc acgtgaccat cgcgagcgat     2280

cgttggaccg cgctgccgac cggtaccccg gtgccggcgt acgacgttct gggtagctat     2340

gtggagctga gccaaccggc gaccaaacgt ggtatcctgc gtctggcgga tgcggcggaa     2400

gatgaggcga ccaaggcgga actgcaaaaa ctggcgggtg atctgtacac cagcgagatt     2460

agcctgaaac gtgcgagcgt tctggacctg ctggatcgtt tcccgagcat cagcctgccg     2520

ttcggtacct ttctgagcct gctgccgccg attcgtccgc gtcaatacag catcagcagc     2580

agcccgctga acgacccgag ccgtgcgacc ctgacctata gcctgctgga tagcccgagc     2640

ctggcgaacc cgagccgtcg tttcgtgggc gttgcgacca gctacctgag cagcctggtt     2700

cgtggtgaca agctgctggt gagcgttcgt ccgacccaca ccgcgtttcg tctgccggac     2760

gaagataaaa tgggtgaaac cgcgatcatt tgcgtgggtg cgggtagcgg tctggcgccg     2820

ttccgtggtt ttatccagga acgtgcggcg ctgctggcga aaggtaccca actggcggcg     2880

gcgctgctgt tctacggttg ccgtagcccg gagaaggacg atctgtatcg tgacgaattc     2940

gataaatggc aagagagcgg tgcggtggat gttcgtcgtg cgtttagccg tgttgatagc     3000

gacgataccg aggcgcgtgg ttgccgtcac gttcaggacc gtctgtggca cgatcgtgaa     3060

gaggtgaagg cgctgtggga ccgtggcgcg cgtgtgtacg tttgcggtag ccgtcaagtg     3120

ggcgaaggtg ttaaaaccgc gatgggccgt atcgtgctgg gtgaagagga cgcggaggat     3180

gcgatcagca agtggtatga aaccgtgcgt aatgaccgtt atgcgaccga tgtgttcgac     3240

taa                                                                   3243


<210>  2
<211>  1251
<212>  DNA
<213>  Escherichia coli

<400>  2
atggaaagta aagtagttgt tccggcacaa ggcaagaaga tcaccctgca aaacggcaaa       60

ctcaacgttc ctgaaaatcc gattatccct tacattgaag gtgatggaat cggtgtagat      120

gtaaccccag ccatgctgaa agtggtcgac gctgcagtcg agaaagccta taaaggcgag      180

cgtaaaatct cctggatgga aatttacacc ggtgaaaaat ccacacaggt ttatggtcag      240

gacgtctggc tgcctgctga aactcttgat ctgattcgtg aatatcgcgt tgccattaaa      300

ggtccgctga ccactccggt tggtggcggt attcgctctc tgaacgttgc cctgcgccag      360

gaactggatc tctacatctg cctgcgtccg gtacgttact atcagggcac tccaagcccg      420

gttaaacacc ctgaactgac cgatatggtt atcttccgtg aaaactcgga agacatttat      480

gcgggtatcg aatggaaagc agactctgcc gacgccgaga aagtgattaa attcctgcgt      540

gaagagatgg gggtgaagaa aattcgcttc ccggaacatt gtggtatcgg tattaagccg      600

tgttcggaag aaggcaccaa acgtctggtt cgtgcagcga tcgaatacgc aattgctaac      660

gatcgtgact ctgtgactct ggtgcacaaa ggcaacatca tgaagttcac cgaaggagcg      720

tttaaagact ggggctacca gctggcgcgt gaagagtttg gcggtgaact gatcgacggt      780

ggcccgtggc tgaaagttaa aaacccgaac actggcaaag agatcgtcat taaagacgtg      840

attgctgatg cattcctgca acagatcctg ctgcgtccgg ctgaatatga tgttatcgcc      900

tgtatgaacc tgaacggtga ctacatttct gacgccctgg cagcgcaggt tggcggtatc      960

ggtatcgccc ctggtgcaaa catcggtgac gaatgcgccc tgtttgaagc cacccacggt     1020

actgcgccga aatatgccgg tcaggacaaa gtaaatcctg gctctattat tctctccgct     1080

gagatgatgc tgcgccacat gggttggacc gaagcggctg acttaattgt taaaggtatg     1140

gaaggcgcaa tcaacgcgaa aaccgtaacc tatgacttcg agcgtctgat ggatggcgct     1200

aaactgctga aatgttcaga gtttggtgac gcgatcatcg aaaacatgta a              1251


<210>  3
<211>  1080
<212>  PRT
<213>  Myceliophthora thermophila

<400>  3

Met Ala Asp Lys Thr Thr Glu Thr Val Pro Ile Pro Gly Pro Pro Gly 
1               5                   10                  15      


Leu Pro Leu Val Gly Asn Ala Leu Ala Phe Asp Ser Glu Leu Pro Leu 
            20                  25                  30          


Arg Thr Phe Gln Glu Phe Ala Glu Glu Tyr Gly Glu Ile Tyr Arg Leu 
        35                  40                  45              


Thr Leu Pro Thr Gly Thr Thr Leu Val Val Ser Ser Gln Ala Leu Val 
    50                  55                  60                  


His Glu Leu Cys Asp Asp Lys Arg Phe Lys Lys Pro Val Ala Ala Ala 
65                  70                  75                  80  


Leu Ala Glu Val Arg Asn Gly Val Asn Asp Gly Leu Phe Thr Ala Arg 
                85                  90                  95      


Glu Glu Glu Pro Asn Trp Gly Ile Ala His Arg Ile Leu Met Pro Ala 
            100                 105                 110         


Phe Gly Pro Ala Ser Ile Gln Gly Met Phe Thr Glu Met His Glu Ile 
        115                 120                 125             


Ala Ser Gln Leu Ala Leu Lys Trp Ala Arg His Gly Pro Asp Thr Pro 
    130                 135                 140                 


Ile Phe Val Thr Asp Asp Phe Thr Arg Leu Thr Leu Asp Thr Leu Ala 
145                 150                 155                 160 


Leu Cys Thr Met Asn Phe Arg Phe Asn Ser Tyr Tyr His Asp Glu Leu 
                165                 170                 175     


His Pro Phe Ile Asn Ala Met Gly Asn Phe Leu Thr Glu Ser Gly Ala 
            180                 185                 190         


Arg Ala Met Arg Pro Ala Ile Thr Ser Ile Phe His Gln Ala Ala Asn 
        195                 200                 205             


Arg Lys Tyr Trp Glu Asp Ile Glu Val Leu Arg Lys Thr Ala Gln Gly 
    210                 215                 220                 


Val Leu Asp Thr Arg Arg Lys His Pro Thr Asn Arg Lys Asp Leu Leu 
225                 230                 235                 240 


Ser Ala Met Leu Asp Gly Val Asp Ala Lys Thr Gly Gln Lys Leu Ser 
                245                 250                 255     


Asp Ser Ser Ile Ile Asp Asn Leu Ile Thr Phe Leu Ile Ala Gly His 
            260                 265                 270         


Glu Thr Thr Ser Gly Leu Leu Ser Phe Ala Phe Tyr Leu Leu Ile Lys 
        275                 280                 285             


His Gln Asp Ala Tyr Arg Lys Ala Gln Glu Glu Val Asp Arg Val Ile 
    290                 295                 300                 


Gly Lys Gly Pro Ile Lys Val Glu His Ile Lys Lys Leu Pro Tyr Ile 
305                 310                 315                 320 


Ala Ala Val Leu Arg Glu Thr Leu Arg Leu Cys Pro Thr Ile Pro Ile 
                325                 330                 335     


Ile Asn Arg Ala Ala Lys Gln Asp Glu Val Ile Gly Gly Lys Tyr Ala 
            340                 345                 350         


Val Ala Lys Asp Gln Arg Leu Ala Leu Leu Leu Ala Gln Ser His Leu 
        355                 360                 365             


Asp Pro Ala Val Tyr Gly Glu Thr Ala Lys Gln Phe Ile Pro Glu Arg 
    370                 375                 380                 


Met Leu Asp Glu Asn Phe Glu Arg Leu Asn Arg Glu Tyr Pro Asp Cys 
385                 390                 395                 400 


Trp Lys Pro Phe Gly Thr Gly Met Arg Ala Cys Ile Gly Arg Pro Phe 
                405                 410                 415     


Ala Trp Gln Glu Ala Val Leu Val Met Ala Met Leu Leu Gln Asn Phe 
            420                 425                 430         


Asp Phe Val Leu His Asp Pro Tyr Tyr Glu Leu His Tyr Lys Gln Thr 
        435                 440                 445             


Leu Thr Thr Lys Pro Lys Asp Phe Tyr Met Arg Ala Ile Leu Arg Asp 
    450                 455                 460                 


Gly Leu Thr Ala Thr Glu Leu Glu His Arg Leu Ala Gly Asn Ala Ala 
465                 470                 475                 480 


Ser Val Ala Arg Ser Gly Gly Gly Gly Gly Gly Pro Ser Lys Pro Thr 
                485                 490                 495     


Ala Gln Lys Thr Ser Pro Ala Glu Ala Lys Pro Met Ser Ile Phe Tyr 
            500                 505                 510         


Gly Ser Asn Thr Gly Thr Cys Glu Ser Leu Ala Gln Arg Leu Ala Thr 
        515                 520                 525             


Asp Ala Ala Ser His Gly Tyr Ala Ala Ala Ala Val Glu Pro Leu Asp 
    530                 535                 540                 


Thr Ala Thr Glu Lys Leu Pro Thr Asp Arg Pro Val Val Ile Ile Thr 
545                 550                 555                 560 


Ala Ser Phe Glu Gly Gln Pro Pro Asp Asn Ala Ala Lys Phe Cys Gly 
                565                 570                 575     


Trp Leu Lys Asn Leu Glu Gly Asp Glu Leu Lys Asn Val Ser Tyr Ala 
            580                 585                 590         


Val Phe Gly Cys Gly His His Asp Trp Ser Gln Thr Phe His Arg Ile 
        595                 600                 605             


Pro Lys Leu Val His Gln Thr Met Lys Ala His Gly Ala Ser Pro Ile 
    610                 615                 620                 


Cys Asp Glu Gly Leu Thr Asp Val Ala Glu Gly Asn Met Phe Thr Asp 
625                 630                 635                 640 


Phe Glu Gln Trp Glu Asp Asp Val Phe Trp Pro Ala Val Arg Ala Arg 
                645                 650                 655     


Tyr Gly Ala Ala Gly Ala Val Ala Glu Thr Glu Asp Ala Pro Gly Ser 
            660                 665                 670         


Asp Gly Leu Asn Ile His Phe Ser Ser Pro Arg Ser Ser Thr Leu Arg 
        675                 680                 685             


Gln Asp Val Arg Glu Ala Thr Val Val Gly Glu Ala Leu Leu Thr Ala 
    690                 695                 700                 


Pro Asp Ala Pro Pro Lys Lys His Ile Glu Val Gln Leu Pro Asp Gly 
705                 710                 715                 720 


Ala Thr Tyr Lys Val Gly Asp Tyr Leu Ala Val Leu Pro Val Asn Ser 
                725                 730                 735     


Lys Glu Ser Ile Gly Arg Val Met Arg Lys Phe Gln Leu Ser Trp Asp 
            740                 745                 750         


Ser His Val Thr Ile Ala Ser Asp Arg Trp Thr Ala Leu Pro Thr Gly 
        755                 760                 765             


Thr Pro Val Pro Ala Tyr Asp Val Leu Gly Ser Tyr Val Glu Leu Ser 
    770                 775                 780                 


Gln Pro Ala Thr Lys Arg Gly Ile Leu Arg Leu Ala Asp Ala Ala Glu 
785                 790                 795                 800 


Asp Glu Ala Thr Lys Ala Glu Leu Gln Lys Leu Ala Gly Asp Leu Tyr 
                805                 810                 815     


Thr Ser Glu Ile Ser Leu Lys Arg Ala Ser Val Leu Asp Leu Leu Asp 
            820                 825                 830         


Arg Phe Pro Ser Ile Ser Leu Pro Phe Gly Thr Phe Leu Ser Leu Leu 
        835                 840                 845             


Pro Pro Ile Arg Pro Arg Gln Tyr Ser Ile Ser Ser Ser Pro Leu Asn 
    850                 855                 860                 


Asp Pro Ser Arg Ala Thr Leu Thr Tyr Ser Leu Leu Asp Ser Pro Ser 
865                 870                 875                 880 


Leu Ala Asn Pro Ser Arg Arg Phe Val Gly Val Ala Thr Ser Tyr Leu 
                885                 890                 895     


Ser Ser Leu Val Arg Gly Asp Lys Leu Leu Val Ser Val Arg Pro Thr 
            900                 905                 910         


His Thr Ala Phe Arg Leu Pro Asp Glu Asp Lys Met Gly Glu Thr Ala 
        915                 920                 925             


Ile Ile Cys Val Gly Ala Gly Ser Gly Leu Ala Pro Phe Arg Gly Phe 
    930                 935                 940                 


Ile Gln Glu Arg Ala Ala Leu Leu Ala Lys Gly Thr Gln Leu Ala Ala 
945                 950                 955                 960 


Ala Leu Leu Phe Tyr Gly Cys Arg Ser Pro Glu Lys Asp Asp Leu Tyr 
                965                 970                 975     


Arg Asp Glu Phe Asp Lys Trp Gln Glu Ser Gly Ala Val Asp Val Arg 
            980                 985                 990         


Arg Ala Phe Ser Arg Val Asp Ser  Asp Asp Thr Glu Ala  Arg Gly Cys 
        995                 1000                 1005             


Arg His  Val Gln Asp Arg Leu  Trp His Asp Arg Glu  Glu Val Lys 
    1010                 1015                 1020             


Ala Leu  Trp Asp Arg Gly Ala  Arg Val Tyr Val Cys  Gly Ser Arg 
    1025                 1030                 1035             


Gln Val  Gly Glu Gly Val Lys  Thr Ala Met Gly Arg  Ile Val Leu 
    1040                 1045                 1050             


Gly Glu  Glu Asp Ala Glu Asp  Ala Ile Ser Lys Trp  Tyr Glu Thr 
    1055                 1060                 1065             


Val Arg  Asn Asp Arg Tyr Ala  Thr Asp Val Phe Asp  
    1070                 1075                 1080 


<210>  4
<211>  416
<212>  PRT
<213>  Escherichia coli

<400>  4

Met Glu Ser Lys Val Val Val Pro Ala Gln Gly Lys Lys Ile Thr Leu 
1               5                   10                  15      


Gln Asn Gly Lys Leu Asn Val Pro Glu Asn Pro Ile Ile Pro Tyr Ile 
            20                  25                  30          


Glu Gly Asp Gly Ile Gly Val Asp Val Thr Pro Ala Met Leu Lys Val 
        35                  40                  45              


Val Asp Ala Ala Val Glu Lys Ala Tyr Lys Gly Glu Arg Lys Ile Ser 
    50                  55                  60                  


Trp Met Glu Ile Tyr Thr Gly Glu Lys Ser Thr Gln Val Tyr Gly Gln 
65                  70                  75                  80  


Asp Val Trp Leu Pro Ala Glu Thr Leu Asp Leu Ile Arg Glu Tyr Arg 
                85                  90                  95      


Val Ala Ile Lys Gly Pro Leu Thr Thr Pro Val Gly Gly Gly Ile Arg 
            100                 105                 110         


Ser Leu Asn Val Ala Leu Arg Gln Glu Leu Asp Leu Tyr Ile Cys Leu 
        115                 120                 125             


Arg Pro Val Arg Tyr Tyr Gln Gly Thr Pro Ser Pro Val Lys His Pro 
    130                 135                 140                 


Glu Leu Thr Asp Met Val Ile Phe Arg Glu Asn Ser Glu Asp Ile Tyr 
145                 150                 155                 160 


Ala Gly Ile Glu Trp Lys Ala Asp Ser Ala Asp Ala Glu Lys Val Ile 
                165                 170                 175     


Lys Phe Leu Arg Glu Glu Met Gly Val Lys Lys Ile Arg Phe Pro Glu 
            180                 185                 190         


His Cys Gly Ile Gly Ile Lys Pro Cys Ser Glu Glu Gly Thr Lys Arg 
        195                 200                 205             


Leu Val Arg Ala Ala Ile Glu Tyr Ala Ile Ala Asn Asp Arg Asp Ser 
    210                 215                 220                 


Val Thr Leu Val His Lys Gly Asn Ile Met Lys Phe Thr Glu Gly Ala 
225                 230                 235                 240 


Phe Lys Asp Trp Gly Tyr Gln Leu Ala Arg Glu Glu Phe Gly Gly Glu 
                245                 250                 255     


Leu Ile Asp Gly Gly Pro Trp Leu Lys Val Lys Asn Pro Asn Thr Gly 
            260                 265                 270         


Lys Glu Ile Val Ile Lys Asp Val Ile Ala Asp Ala Phe Leu Gln Gln 
        275                 280                 285             


Ile Leu Leu Arg Pro Ala Glu Tyr Asp Val Ile Ala Cys Met Asn Leu 
    290                 295                 300                 


Asn Gly Asp Tyr Ile Ser Asp Ala Leu Ala Ala Gln Val Gly Gly Ile 
305                 310                 315                 320 


Gly Ile Ala Pro Gly Ala Asn Ile Gly Asp Glu Cys Ala Leu Phe Glu 
                325                 330                 335     


Ala Thr His Gly Thr Ala Pro Lys Tyr Ala Gly Gln Asp Lys Val Asn 
            340                 345                 350         


Pro Gly Ser Ile Ile Leu Ser Ala Glu Met Met Leu Arg His Met Gly 
        355                 360                 365             


Trp Thr Glu Ala Ala Asp Leu Ile Val Lys Gly Met Glu Gly Ala Ile 
    370                 375                 380                 


Asn Ala Lys Thr Val Thr Tyr Asp Phe Glu Arg Leu Met Asp Gly Ala 
385                 390                 395                 400 


Lys Leu Leu Lys Cys Ser Glu Phe Gly Asp Ala Ile Ile Glu Asn Met 
                405                 410                 415     


<210>  5
<211>  3573
<212>  DNA
<213>  Unknown

<220>
<223>  Umbelopsis isabellina

<400>  5
atgagcacca ccaccctgat tgtggccatt aagggtaccg ataatgttga acgtgatggc       60

gcaattgttc gtctggatgc cggcgccagt atggaaaccc tgcgtccgcg cattgcagaa      120

aaactggcca ttagcagtgg cattgaagat ctgattctgg aagatgcaaa tggtgacaat      180

ctgaccacca ttgatcaggt tcgtaaacag cagaccgttt ttgttaatct ggaagatcag      240

attaagctgc cggcagttcc ggcccatacc ctgccgtatt ttggtaatct gtatcagctg      300

ctgccggata tgctggccgg ttggcgcaaa ctgtttgatg aatatggtcc ggttgttaaa      360

gtgaatctgc tgggcaatga aattattggt accaatgatc cggccgtggc cgaactgtgg      420

gttaaagaaa gcgaatattt taccaaaaag atctacggtg gcctgcagga agttaaaagt      480

tttggcggcc agggtctgtt taccaccgat agcgatgata tggattggaa actggcacat      540

aaactgctga tgccggcatt ttcaccgcgc gccattaagg tttatcagca tgaaatgagc      600

gtgattggtc tgcagaccat taaggttttt gaacagtata gcccggatga agaagtggaa      660

attctgcatt ggaccaccaa tctgaccttt gaaaccattg gcaaagttgg ttttggctat      720

gattttcatc tgctggatga tcgttatggc gaaaatcatc cgtttattga agcaatgggt      780

tattgcatga aacagagctt tgcccgtggc acccagagca aactgattaa gtatctgccg      840

attgaagcca atcgtcgcta tgatcgcagt ctgaatctga tgcatagcat tgttgatgaa      900

gttattaccc agcgtaaaag ccatccgcat gcaagcgaag ataataagga tctgctggat      960

tttatgctga ccgcacgtga tgaaaataat ctgggcctga gcgatgaaaa tattcgcgat     1020

caggttatta cctttctgat tgccggccat gaaaccacca gcaataccct ggcatggacc     1080

ctgtatgaac tgagtcgcca tccggaaatt gaacagaaaa ttctgcagga agtggtgaat     1140

ctgggcatta ccaccgattc actgccgacc agcgaacaga gcagtagcat gaaatatacc     1200

tatcaggtgc tgaaagaaac cctgcgcatg tatagtccgc tgcgtgcact ggcaaaatat     1260

tgcaaaaaag atattgtggt gccgggcggc tatcagatta aggcaggcga tcgtgtggcc     1320

gttcagctga atagcctgca ttataatgaa aaagtttacc cgaatccgac ccagtatgat     1380

ccgagtcgct ggaccccgga agaagaacag aaacgcagtc gctttgcctg gctgccgttt     1440

agcaccggcc cgcgtagctg cattggtatg gcactggccc tgcaggaagc aaaaaccatt     1500

ctggcaatga ttctgctgaa atttcgcttt gtttatgatg gtccgccgat tggttatgat     1560

ccgaaaagcc cgaccattcg tccgctgaat ctgatgatga aaattctgcc gcgtgataaa     1620

ctgccgagcc cgaccgccga taataagctg accccggttg gcagtccgaa tattggtaaa     1680

gcccagatgg caatgccgac cgcagccacc aatgttggta ccgccgaact gccgccggtt     1740

ttctttctgt atggtaccca gaccggtacc gcccaggatt atgcaagtca gctggcaagc     1800

caggccaaaa gttttggttt taaagatatt accctgtgtg aaatggataa atgggaagtg     1860

ctgcagagcg gcaaatatac cggtgccaaa gaagaaaaag atacccgtgc cctggttgtt     1920

atttgtaccg caacctataa tggcagcccg ccggatagtg cagaaaattt tgataaattc     1980

attaccgaca ccagcaaaga taatgatctg ccgctgaaag gcctgctgta taccgtgttt     2040

ggcctgggca atcgtaattg gcgcacctat cagcagtttc cgattaagtg cgatgcccgt     2100

ctggatgaac tgggtggtga acgcttttat gatctgggca gcggcaatgc cgataaagat     2160

atggatggtg aatttcatga ttggtgcgcc catttttgga cccataccct gacctattat     2220

ggcattgcaa cccatgaagg tagtgcaagt ctggtgccgg gccagaaaac caccgaagat     2280

ccgaccagcg gtctggaaat tcgttttatt ccgccgagcg aaagcgaagc ctgggataaa     2340

gccgataaaa atgttaatgg tgactataat gcggaaattc aggtgaatcg cgaactgcag     2400

aatattgaac gtagtaaacg tagcacccgc catattgaaa ttgatattag taaactgcag     2460

agcccggata ccgataaaca tcgttatctg accggtgacc atctggaagt gtatccggaa     2520

aatgccgata atgtggtgga aagcattgcc ctgggctttg gtctgattct ggatagtgtg     2580

tttgaaatta ccagcgtgga taaaagtacc gtgagtagtc gtagcctggc agccagtatt     2640

accggcccgt gtaccgttcg taatgccctg aaatattatg ccgatatata tagtgccccg     2700

agccgttatc tgctggcctt tttcgcagca cgcctgcagg atacccatcc ggatgttgcc     2760

gcaagtttta gcgatgtgat tattccgggt gaaaatggcc agaaagccta taatgaattc     2820

attcagaaat accgtaacct gctggatctg cagcgcggct ttccgctgaa agaactggat     2880

ctgaaagaat ttctgtgcgc agttagcgtt atgcagccgc gccgttatag cattgccagc     2940

agcccgctga aagcagaaga taccgcatat ctggcagttg gtgtggtgga tgatgtgttt     3000

gcaaatcgtc attattatgg cctggccagt ggctatctgg cccgcagcca gccgccgaca     3060

ccgattcgtg cacgtattaa gagtagcaaa agtacctttg gcctgccgga aaatccggaa     3120

accccgatta ttatgattgg tgccggtacc ggtattagtc cgtttatggg ctttatgcag     3180

gaacgcgaaa tgctgaaaag caaagcagaa gcccatctgt ttttcggctg ccgccatccg     3240

gatgaagatt ttatctatcg tagcgttttt gaaggctatg aaaaaagcgg cgttattacc     3300

aaactgtatc cggcctttag ccgtctgggt gaagataatc cgcgcaaata tgttcagcat     3360

cagctgatgg caaatgccgg caaaatttgg accctgctga ccaatggcgc aaatgtgtat     3420

gtttgcggca gtggcccgat gagccgcgat gtgcgtcgta gctttgaact gatggcaaaa     3480

agttttggag gcgccaaaac cgatgatgaa gcatgcgaaa aactgctgga attcatgagc     3540

gcaggtcgtt ataatgaaga tgtgtggggt taa                                  3573


<210>  6
<211>  1190
<212>  PRT
<213>  Unknown

<220>
<223>  Umbelopsis isabellina

<400>  6

Met Ser Thr Thr Thr Leu Ile Val Ala Ile Lys Gly Thr Asp Asn Val 
1               5                   10                  15      


Glu Arg Asp Gly Ala Ile Val Arg Leu Asp Ala Gly Ala Ser Met Glu 
            20                  25                  30          


Thr Leu Arg Pro Arg Ile Ala Glu Lys Leu Ala Ile Ser Ser Gly Ile 
        35                  40                  45              


Glu Asp Leu Ile Leu Glu Asp Ala Asn Gly Asp Asn Leu Thr Thr Ile 
    50                  55                  60                  


Asp Gln Val Arg Lys Gln Gln Thr Val Phe Val Asn Leu Glu Asp Gln 
65                  70                  75                  80  


Ile Lys Leu Pro Ala Val Pro Ala His Thr Leu Pro Tyr Phe Gly Asn 
                85                  90                  95      


Leu Tyr Gln Leu Leu Pro Asp Met Leu Ala Gly Trp Arg Lys Leu Phe 
            100                 105                 110         


Asp Glu Tyr Gly Pro Val Val Lys Val Asn Leu Leu Gly Asn Glu Ile 
        115                 120                 125             


Ile Gly Thr Asn Asp Pro Ala Val Ala Glu Leu Trp Val Lys Glu Ser 
    130                 135                 140                 


Glu Tyr Phe Thr Lys Lys Ile Tyr Gly Gly Leu Gln Glu Val Lys Ser 
145                 150                 155                 160 


Phe Gly Gly Gln Gly Leu Phe Thr Thr Asp Ser Asp Asp Met Asp Trp 
                165                 170                 175     


Lys Leu Ala His Lys Leu Leu Met Pro Ala Phe Ser Pro Arg Ala Ile 
            180                 185                 190         


Lys Val Tyr Gln His Glu Met Ser Val Ile Gly Leu Gln Thr Ile Lys 
        195                 200                 205             


Val Phe Glu Gln Tyr Ser Pro Asp Glu Glu Val Glu Ile Leu His Trp 
    210                 215                 220                 


Thr Thr Asn Leu Thr Phe Glu Thr Ile Gly Lys Val Gly Phe Gly Tyr 
225                 230                 235                 240 


Asp Phe His Leu Leu Asp Asp Arg Tyr Gly Glu Asn His Pro Phe Ile 
                245                 250                 255     


Glu Ala Met Gly Tyr Cys Met Lys Gln Ser Phe Ala Arg Gly Thr Gln 
            260                 265                 270         


Ser Lys Leu Ile Lys Tyr Leu Pro Ile Glu Ala Asn Arg Arg Tyr Asp 
        275                 280                 285             


Arg Ser Leu Asn Leu Met His Ser Ile Val Asp Glu Val Ile Thr Gln 
    290                 295                 300                 


Arg Lys Ser His Pro His Ala Ser Glu Asp Asn Lys Asp Leu Leu Asp 
305                 310                 315                 320 


Phe Met Leu Thr Ala Arg Asp Glu Asn Asn Leu Gly Leu Ser Asp Glu 
                325                 330                 335     


Asn Ile Arg Asp Gln Val Ile Thr Phe Leu Ile Ala Gly His Glu Thr 
            340                 345                 350         


Thr Ser Asn Thr Leu Ala Trp Thr Leu Tyr Glu Leu Ser Arg His Pro 
        355                 360                 365             


Glu Ile Glu Gln Lys Ile Leu Gln Glu Val Val Asn Leu Gly Ile Thr 
    370                 375                 380                 


Thr Asp Ser Leu Pro Thr Ser Glu Gln Ser Ser Ser Met Lys Tyr Thr 
385                 390                 395                 400 


Tyr Gln Val Leu Lys Glu Thr Leu Arg Met Tyr Ser Pro Leu Arg Ala 
                405                 410                 415     


Leu Ala Lys Tyr Cys Lys Lys Asp Ile Val Val Pro Gly Gly Tyr Gln 
            420                 425                 430         


Ile Lys Ala Gly Asp Arg Val Ala Val Gln Leu Asn Ser Leu His Tyr 
        435                 440                 445             


Asn Glu Lys Val Tyr Pro Asn Pro Thr Gln Tyr Asp Pro Ser Arg Trp 
    450                 455                 460                 


Thr Pro Glu Glu Glu Gln Lys Arg Ser Arg Phe Ala Trp Leu Pro Phe 
465                 470                 475                 480 


Ser Thr Gly Pro Arg Ser Cys Ile Gly Met Ala Leu Ala Leu Gln Glu 
                485                 490                 495     


Ala Lys Thr Ile Leu Ala Met Ile Leu Leu Lys Phe Arg Phe Val Tyr 
            500                 505                 510         


Asp Gly Pro Pro Ile Gly Tyr Asp Pro Lys Ser Pro Thr Ile Arg Pro 
        515                 520                 525             


Leu Asn Leu Met Met Lys Ile Leu Pro Arg Asp Lys Leu Pro Ser Pro 
    530                 535                 540                 


Thr Ala Asp Asn Lys Leu Thr Pro Val Gly Ser Pro Asn Ile Gly Lys 
545                 550                 555                 560 


Ala Gln Met Ala Met Pro Thr Ala Ala Thr Asn Val Gly Thr Ala Glu 
                565                 570                 575     


Leu Pro Pro Val Phe Phe Leu Tyr Gly Thr Gln Thr Gly Thr Ala Gln 
            580                 585                 590         


Asp Tyr Ala Ser Gln Leu Ala Ser Gln Ala Lys Ser Phe Gly Phe Lys 
        595                 600                 605             


Asp Ile Thr Leu Cys Glu Met Asp Lys Trp Glu Val Leu Gln Ser Gly 
    610                 615                 620                 


Lys Tyr Thr Gly Ala Lys Glu Glu Lys Asp Thr Arg Ala Leu Val Val 
625                 630                 635                 640 


Ile Cys Thr Ala Thr Tyr Asn Gly Ser Pro Pro Asp Ser Ala Glu Asn 
                645                 650                 655     


Phe Asp Lys Phe Ile Thr Asp Thr Ser Lys Asp Asn Asp Leu Pro Leu 
            660                 665                 670         


Lys Gly Leu Leu Tyr Thr Val Phe Gly Leu Gly Asn Arg Asn Trp Arg 
        675                 680                 685             


Thr Tyr Gln Gln Phe Pro Ile Lys Cys Asp Ala Arg Leu Asp Glu Leu 
    690                 695                 700                 


Gly Gly Glu Arg Phe Tyr Asp Leu Gly Ser Gly Asn Ala Asp Lys Asp 
705                 710                 715                 720 


Met Asp Gly Glu Phe His Asp Trp Cys Ala His Phe Trp Thr His Thr 
                725                 730                 735     


Leu Thr Tyr Tyr Gly Ile Ala Thr His Glu Gly Ser Ala Ser Leu Val 
            740                 745                 750         


Pro Gly Gln Lys Thr Thr Glu Asp Pro Thr Ser Gly Leu Glu Ile Arg 
        755                 760                 765             


Phe Ile Pro Pro Ser Glu Ser Glu Ala Trp Asp Lys Ala Asp Lys Asn 
    770                 775                 780                 


Val Asn Gly Asp Tyr Asn Ala Glu Ile Gln Val Asn Arg Glu Leu Gln 
785                 790                 795                 800 


Asn Ile Glu Arg Ser Lys Arg Ser Thr Arg His Ile Glu Ile Asp Ile 
                805                 810                 815     


Ser Lys Leu Gln Ser Pro Asp Thr Asp Lys His Arg Tyr Leu Thr Gly 
            820                 825                 830         


Asp His Leu Glu Val Tyr Pro Glu Asn Ala Asp Asn Val Val Glu Ser 
        835                 840                 845             


Ile Ala Leu Gly Phe Gly Leu Ile Leu Asp Ser Val Phe Glu Ile Thr 
    850                 855                 860                 


Ser Val Asp Lys Ser Thr Val Ser Ser Arg Ser Leu Ala Ala Ser Ile 
865                 870                 875                 880 


Thr Gly Pro Cys Thr Val Arg Asn Ala Leu Lys Tyr Tyr Ala Asp Ile 
                885                 890                 895     


Tyr Ser Ala Pro Ser Arg Tyr Leu Leu Ala Phe Phe Ala Ala Arg Leu 
            900                 905                 910         


Gln Asp Thr His Pro Asp Val Ala Ala Ser Phe Ser Asp Val Ile Ile 
        915                 920                 925             


Pro Gly Glu Asn Gly Gln Lys Ala Tyr Asn Glu Phe Ile Gln Lys Tyr 
    930                 935                 940                 


Arg Asn Leu Leu Asp Leu Gln Arg Gly Phe Pro Leu Lys Glu Leu Asp 
945                 950                 955                 960 


Leu Lys Glu Phe Leu Cys Ala Val Ser Val Met Gln Pro Arg Arg Tyr 
                965                 970                 975     


Ser Ile Ala Ser Ser Pro Leu Lys Ala Glu Asp Thr Ala Tyr Leu Ala 
            980                 985                 990         


Val Gly Val Val Asp Asp Val Phe  Ala Asn Arg His Tyr  Tyr Gly Leu 
        995                 1000                 1005             


Ala Ser  Gly Tyr Leu Ala Arg  Ser Gln Pro Pro Thr  Pro Ile Arg 
    1010                 1015                 1020             


Ala Arg  Ile Lys Ser Ser Lys  Ser Thr Phe Gly Leu  Pro Glu Asn 
    1025                 1030                 1035             


Pro Glu  Thr Pro Ile Ile Met  Ile Gly Ala Gly Thr  Gly Ile Ser 
    1040                 1045                 1050             


Pro Phe  Met Gly Phe Met Gln  Glu Arg Glu Met Leu  Lys Ser Lys 
    1055                 1060                 1065             


Ala Glu  Ala His Leu Phe Phe  Gly Cys Arg His Pro  Asp Glu Asp 
    1070                 1075                 1080             


Phe Ile  Tyr Arg Ser Val Phe  Glu Gly Tyr Glu Lys  Ser Gly Val 
    1085                 1090                 1095             


Ile Thr  Lys Leu Tyr Pro Ala  Phe Ser Arg Leu Gly  Glu Asp Asn 
    1100                 1105                 1110             


Pro Arg  Lys Tyr Val Gln His  Gln Leu Met Ala Asn  Ala Gly Lys 
    1115                 1120                 1125             


Ile Trp  Thr Leu Leu Thr Asn  Gly Ala Asn Val Tyr  Val Cys Gly 
    1130                 1135                 1140             


Ser Gly  Pro Met Ser Arg Asp  Val Arg Arg Ser Phe  Glu Leu Met 
    1145                 1150                 1155             


Ala Lys  Ser Phe Gly Gly Ala  Lys Thr Asp Asp Glu  Ala Cys Glu 
    1160                 1165                 1170             


Lys Leu  Leu Glu Phe Met Ser  Ala Gly Arg Tyr Asn  Glu Asp Val 
    1175                 1180                 1185             


Trp Gly  
    1190 


<210>  7
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  7
ggaattccat atggaaagta aagtagttgt                                        30


<210>  8
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  8
ccgctcgagt tacatgtttt cgatgatcgc gt                                     32


