                         SEQUENCE LISTING

<110>  Regeneron Pharmaceuticals, Inc.
 
<120>  CRISPR/CAS DROPOUT SCREENING PLATFORM TO REVEAL GENETIC 
       VULNERABILITIES ASSOCIATED WITH TAU AGGREGATION

<130>  057766-544640

<150>  US 62/820,101
<151>  2019-03-18

<160>  43    

<170>  PatentIn version 3.5

<210>  1
<211>  31
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  1

Gln Thr Ala Pro Val Pro Met Pro Asp Leu Lys Asn Val Lys Ser Lys 
1               5                   10                  15      


Ile Gly Ser Thr Glu Asn Leu Lys His Gln Pro Gly Gly Gly Lys 
            20                  25                  30      


<210>  2
<211>  31
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  2

Val Gln Ile Ile Asn Lys Lys Leu Asp Leu Ser Asn Val Gln Ser Lys 
1               5                   10                  15      


Cys Gly Ser Lys Asp Asn Ile Lys His Val Pro Gly Gly Gly Ser 
            20                  25                  30      


<210>  3
<211>  31
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  3

Val Gln Ile Val Tyr Lys Pro Val Asp Leu Ser Lys Val Thr Ser Lys 
1               5                   10                  15      


Cys Gly Ser Leu Gly Asn Ile His His Lys Pro Gly Gly Gly Gln 
            20                  25                  30      


<210>  4
<211>  32
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  4

Val Glu Val Lys Ser Glu Lys Leu Asp Phe Lys Asp Arg Val Gln Ser 
1               5                   10                  15      


Lys Ile Gly Ser Leu Asp Asn Ile Thr His Val Pro Gly Gly Gly Asn 
            20                  25                  30          


<210>  5
<211>  93
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  5
cagacagccc ccgtgcccat gccagacctg aagaatgtca agtccaagat cggctccact       60

gagaacctga agcaccagcc gggaggcggg aag                                    93


<210>  6
<211>  93
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  6
gtgcagataa ttaataagaa gctggatctt agcaacgtcc agtccaagtg tggctcaaag       60

gataatatca aacacgtccc gggaggcggc agt                                    93


<210>  7
<211>  93
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  7
gtgcaaatag tctacaaacc agttgacctg agcaaggtga cctccaagtg tggctcatta       60

ggcaacatcc atcataaacc aggaggtggc cag                                    93


<210>  8
<211>  96
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  8
gtggaagtaa aatctgagaa gcttgacttc aaggacagag tccagtcgaa gattgggtcc       60

ctggacaata tcacccacgt ccctggcgga ggaaat                                 96


<210>  9
<211>  133
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  9

Leu Gln Thr Ala Pro Val Pro Met Pro Asp Leu Lys Asn Val Lys Ser 
1               5                   10                  15      


Lys Ile Gly Ser Thr Glu Asn Leu Lys His Gln Pro Gly Gly Gly Lys 
            20                  25                  30          


Val Gln Ile Ile Asn Lys Lys Leu Asp Leu Ser Asn Val Gln Ser Lys 
        35                  40                  45              


Cys Gly Ser Lys Asp Asn Ile Lys His Val Pro Gly Gly Gly Ser Val 
    50                  55                  60                  


Gln Ile Val Tyr Lys Pro Val Asp Leu Ser Lys Val Thr Ser Lys Cys 
65                  70                  75                  80  


Gly Ser Leu Gly Asn Ile His His Lys Pro Gly Gly Gly Gln Val Glu 
                85                  90                  95      


Val Lys Ser Glu Lys Leu Asp Phe Lys Asp Arg Val Gln Ser Lys Ile 
            100                 105                 110         


Gly Ser Leu Asp Asn Ile Thr His Val Pro Gly Gly Gly Asn Lys Lys 
        115                 120                 125             


Ile Glu Thr His Lys 
    130             


<210>  10
<211>  399
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  10
ctgcagacag cccccgtgcc catgccagac ctgaagaatg tcaagtccaa gatcggctcc       60

actgagaacc tgaagcacca gccgggaggc gggaaggtgc agataattaa taagaagctg      120

gatcttagca acgtccagtc caagtgtggc tcaaaggata atatcaaaca cgtcccggga      180

ggcggcagtg tgcaaatagt ctacaaacca gttgacctga gcaaggtgac ctccaagtgt      240

ggctcattag gcaacatcca tcataaacca ggaggtggcc aggtggaagt aaaatctgag      300

aagcttgact tcaaggacag agtccagtcg aagattgggt ccctggacaa tatcacccac      360

gtccctggcg gaggaaataa aaagattgaa acccacaag                             399


<210>  11
<211>  133
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  11

Leu Gln Thr Ala Pro Val Pro Met Pro Asp Leu Lys Asn Val Lys Ser 
1               5                   10                  15      


Lys Ile Gly Ser Thr Glu Asn Leu Lys His Gln Pro Gly Gly Gly Lys 
            20                  25                  30          


Val Gln Ile Ile Asn Lys Lys Leu Asp Leu Ser Asn Val Gln Ser Lys 
        35                  40                  45              


Cys Gly Ser Lys Asp Asn Ile Lys His Val Ser Gly Gly Gly Ser Val 
    50                  55                  60                  


Gln Ile Val Tyr Lys Pro Val Asp Leu Ser Lys Val Thr Ser Lys Cys 
65                  70                  75                  80  


Gly Ser Leu Gly Asn Ile His His Lys Pro Gly Gly Gly Gln Val Glu 
                85                  90                  95      


Val Lys Ser Glu Lys Leu Asp Phe Lys Asp Arg Val Gln Ser Lys Ile 
            100                 105                 110         


Gly Ser Leu Asp Asn Ile Thr His Val Pro Gly Gly Gly Asn Lys Lys 
        115                 120                 125             


Ile Glu Thr His Lys 
    130             


<210>  12
<211>  399
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  12
ctgcagacag cccccgtgcc catgccagac ctgaagaatg tcaagtccaa gatcggctcc       60

actgagaacc tgaagcacca gccgggaggc gggaaggtgc agataattaa taagaagctg      120

gatcttagca acgtccagtc caagtgtggc tcaaaggata atatcaaaca cgtctcggga      180

ggcggcagtg tgcaaatagt ctacaaacca gttgacctga gcaaggtgac ctccaagtgt      240

ggctcattag gcaacatcca tcataaacca ggaggtggcc aggtggaagt aaaatctgag      300

aagcttgact tcaaggacag agtccagtcg aagattgggt ccctggacaa tatcacccac      360

gtccctggcg gaggaaataa aaagattgaa acccacaag                             399


<210>  13
<211>  238
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  13

Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val 
1               5                   10                  15      


Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly Glu 
            20                  25                  30          


Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile Cys 
        35                  40                  45              


Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Leu 
    50                  55                  60                  


Thr Trp Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys Gln 
65                  70                  75                  80  


His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg 
                85                  90                  95      


Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val 
            100                 105                 110         


Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile 
        115                 120                 125             


Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn 
    130                 135                 140                 


Tyr Ile Ser His Asn Val Tyr Ile Thr Ala Asp Lys Gln Lys Asn Gly 
145                 150                 155                 160 


Ile Lys Ala Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser Val 
                165                 170                 175     


Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro 
            180                 185                 190         


Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Ala Leu Ser 
        195                 200                 205             


Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe Val 
    210                 215                 220                 


Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys 
225                 230                 235             


<210>  14
<211>  714
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  14
gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc       60

gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc      120

aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc      180

gtgaccaccc tgacctgggg cgtgcagtgc ttcagccgct accccgacca catgaagcag      240

cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc      300

aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg      360

aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag      420

ctggagtaca actacatcag ccacaacgtc tatatcaccg ccgacaagca gaagaacggc      480

atcaaggcca acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac      540

cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac      600

ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg      660

ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caag            714


<210>  15
<211>  236
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  15

Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val 
1               5                   10                  15      


Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly Glu 
            20                  25                  30          


Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile Cys 
        35                  40                  45              


Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Phe 
    50                  55                  60                  


Gly Tyr Gly Leu Gln Cys Phe Ala Arg Tyr Pro Asp His Met Lys Gln 
65                  70                  75                  80  


His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg 
                85                  90                  95      


Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val 
            100                 105                 110         


Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile 
        115                 120                 125             


Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn 
    130                 135                 140                 


Tyr Asn Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn Gly 
145                 150                 155                 160 


Ile Lys Val Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser Val 
                165                 170                 175     


Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro 
            180                 185                 190         


Val Leu Leu Pro Asp Asn His Tyr Leu Ser Tyr Gln Ser Ala Leu Ser 
        195                 200                 205             


Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe Val 
    210                 215                 220                 


Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu 
225                 230                 235     


<210>  16
<211>  708
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  16
gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc       60

gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc      120

aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc      180

gtgaccacct tcggctacgg cctgcagtgc ttcgcccgct accccgacca catgaagcag      240

cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc      300

aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg      360

aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag      420

ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc      480

atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac      540

cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac      600

ctgagctacc agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg      660

ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctg                   708


<210>  17
<211>  77
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  17
guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc cguuaucaac uugaaaaagu       60

ggcaccgagu cggugcu                                                      77


<210>  18
<211>  82
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  18
guuggaacca uucaaaacag cauagcaagu uaaaauaagg cuaguccguu aucaacuuga       60

aaaaguggca ccgagucggu gc                                                82


<210>  19
<211>  76
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  19
guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc cguuaucaac uugaaaaagu       60

ggcaccgagu cggugc                                                       76


<210>  20
<211>  86
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  20
guuuaagagc uaugcuggaa acagcauagc aaguuuaaau aaggcuaguc cguuaucaac       60

uugaaaaagu ggcaccgagu cggugc                                            86


<210>  21
<211>  1392
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<222>  (1)..(1384)
<223>  Cas9

<220>
<221>  MISC_FEATURE
<222>  (1385)..(1392)
<223>  FLAG

<400>  21

Met Asp Lys Lys Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp 
    130                 135                 140                 


Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     


Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         


Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
        195                 200                 205             


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         


Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 


Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             


Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 


Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         


Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 


Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     


Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 


Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 


Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     


Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             


Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 


Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 


His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
                645                 650                 655     


Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             


Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 


Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
705                 710                 715                 720 


His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         


Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             


Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
    770                 775                 780                 


Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 


Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     


Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg 
            820                 825                 830         


Leu Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Leu Lys 
        835                 840                 845             


Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg 
    850                 855                 860                 


Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 


Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys 
                885                 890                 895     


Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp 
            900                 905                 910         


Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             


Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp 
    930                 935                 940                 


Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 


Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg 
                965                 970                 975     


Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         


Val Gly Thr Ala Leu Ile Lys Lys  Tyr Pro Lys Leu Glu  Ser Glu Phe 
        995                 1000                 1005             


Val Tyr  Gly Asp Tyr Lys Val  Tyr Asp Val Arg Lys  Met Ile Ala 
    1010                 1015                 1020             


Lys Ser  Glu Gln Glu Ile Gly  Lys Ala Thr Ala Lys  Tyr Phe Phe 
    1025                 1030                 1035             


Tyr Ser  Asn Ile Met Asn Phe  Phe Lys Thr Glu Ile  Thr Leu Ala 
    1040                 1045                 1050             


Asn Gly  Glu Ile Arg Lys Arg  Pro Leu Ile Glu Thr  Asn Gly Glu 
    1055                 1060                 1065             


Thr Gly  Glu Ile Val Trp Asp  Lys Gly Arg Asp Phe  Ala Thr Val 
    1070                 1075                 1080             


Arg Lys  Val Leu Ser Met Pro  Gln Val Asn Ile Val  Lys Lys Thr 
    1085                 1090                 1095             


Glu Val  Gln Thr Gly Gly Phe  Ser Lys Glu Ser Ile  Leu Pro Lys 
    1100                 1105                 1110             


Arg Asn  Ser Asp Lys Leu Ile  Ala Arg Lys Lys Asp  Trp Asp Pro 
    1115                 1120                 1125             


Lys Lys  Tyr Gly Gly Phe Asp  Ser Pro Thr Val Ala  Tyr Ser Val 
    1130                 1135                 1140             


Leu Val  Val Ala Lys Val Glu  Lys Gly Lys Ser Lys  Lys Leu Lys 
    1145                 1150                 1155             


Ser Val  Lys Glu Leu Leu Gly  Ile Thr Ile Met Glu  Arg Ser Ser 
    1160                 1165                 1170             


Phe Glu  Lys Asn Pro Ile Asp  Phe Leu Glu Ala Lys  Gly Tyr Lys 
    1175                 1180                 1185             


Glu Val  Lys Lys Asp Leu Ile  Ile Lys Leu Pro Lys  Tyr Ser Leu 
    1190                 1195                 1200             


Phe Glu  Leu Glu Asn Gly Arg  Lys Arg Met Leu Ala  Ser Ala Gly 
    1205                 1210                 1215             


Glu Leu  Gln Lys Gly Asn Glu  Leu Ala Leu Pro Ser  Lys Tyr Val 
    1220                 1225                 1230             


Asn Phe  Leu Tyr Leu Ala Ser  His Tyr Glu Lys Leu  Lys Gly Ser 
    1235                 1240                 1245             


Pro Glu  Asp Asn Glu Gln Lys  Gln Leu Phe Val Glu  Gln His Lys 
    1250                 1255                 1260             


His Tyr  Leu Asp Glu Ile Ile  Glu Gln Ile Ser Glu  Phe Ser Lys 
    1265                 1270                 1275             


Arg Val  Ile Leu Ala Asp Ala  Asn Leu Asp Lys Val  Leu Ser Ala 
    1280                 1285                 1290             


Tyr Asn  Lys His Arg Asp Lys  Pro Ile Arg Glu Gln  Ala Glu Asn 
    1295                 1300                 1305             


Ile Ile  His Leu Phe Thr Leu  Thr Asn Leu Gly Ala  Pro Ala Ala 
    1310                 1315                 1320             


Phe Lys  Tyr Phe Asp Thr Thr  Ile Asp Arg Lys Arg  Tyr Thr Ser 
    1325                 1330                 1335             


Thr Lys  Glu Val Leu Asp Ala  Thr Leu Ile His Gln  Ser Ile Thr 
    1340                 1345                 1350             


Gly Leu  Tyr Glu Thr Arg Ile  Asp Leu Ser Gln Leu  Gly Gly Asp 
    1355                 1360                 1365             


Lys Arg  Pro Ala Ala Thr Lys  Lys Ala Gly Gln Ala  Lys Lys Lys 
    1370                 1375                 1380             


Lys Asp  Tyr Lys Asp Asp Asp  Asp Lys 
    1385                 1390         


<210>  22
<211>  4176
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(4152)
<223>  Cas9

<220>
<221>  misc_feature
<222>  (4153)..(4176)
<223>  FLAG

<400>  22
atggacaaga agtacagcat cggcctggac atcggcacca actctgtggg ctgggccgtg       60

atcaccgacg agtacaaggt gcccagcaag aaattcaagg tgctgggcaa caccgaccgg      120

cacagcatca agaagaacct gatcggagcc ctgctgttcg acagcggcga aacagccgag      180

gccacccggc tgaagagaac cgccagaaga agatacacca gacggaagaa ccggatctgc      240

tatctgcaag agatcttcag caacgagatg gccaaggtgg acgacagctt cttccacaga      300

ctggaagagt ccttcctggt ggaagaggat aagaagcacg agcggcaccc catcttcggc      360

aacatcgtgg acgaggtggc ctaccacgag aagtacccca ccatctacca cctgagaaag      420

aaactggtgg acagcaccga caaggccgac ctgcggctga tctatctggc cctggcccac      480

atgatcaagt tccggggcca cttcctgatc gagggcgacc tgaaccccga caacagcgac      540

gtggacaagc tgttcatcca gctggtgcag acctacaacc agctgttcga ggaaaacccc      600

atcaacgcca gcggcgtgga cgccaaggcc atcctgtctg ccagactgag caagagcaga      660

cggctggaaa atctgatcgc ccagctgccc ggcgagaaga agaatggcct gttcggaaac      720

ctgattgccc tgagcctggg cctgaccccc aacttcaaga gcaacttcga cctggccgag      780

gatgccaaac tgcagctgag caaggacacc tacgacgacg acctggacaa cctgctggcc      840

cagatcggcg accagtacgc cgacctgttt ctggccgcca agaacctgtc cgacgccatc      900

ctgctgagcg acatcctgag agtgaacacc gagatcacca aggcccccct gagcgcctct      960

atgatcaaga gatacgacga gcaccaccag gacctgaccc tgctgaaagc tctcgtgcgg     1020

cagcagctgc ctgagaagta caaagagatt ttcttcgacc agagcaagaa cggctacgcc     1080

ggctacattg acggcggagc cagccaggaa gagttctaca agttcatcaa gcccatcctg     1140

gaaaagatgg acggcaccga ggaactgctc gtgaagctga acagagagga cctgctgcgg     1200

aagcagcgga ccttcgacaa cggcagcatc ccccaccaga tccacctggg agagctgcac     1260

gccattctgc ggcggcagga agatttttac ccattcctga aggacaaccg ggaaaagatc     1320

gagaagatcc tgaccttccg catcccctac tacgtgggcc ctctggccag gggaaacagc     1380

agattcgcct ggatgaccag aaagagcgag gaaaccatca ccccctggaa cttcgaggaa     1440

gtggtggaca agggcgcttc cgcccagagc ttcatcgagc ggatgaccaa cttcgataag     1500

aacctgccca acgagaaggt gctgcccaag cacagcctgc tgtacgagta cttcaccgtg     1560

tataacgagc tgaccaaagt gaaatacgtg accgagggaa tgagaaagcc cgccttcctg     1620

agcggcgagc agaaaaaggc catcgtggac ctgctgttca agaccaaccg gaaagtgacc     1680

gtgaagcagc tgaaagagga ctacttcaag aaaatcgagt gcttcgactc cgtggaaatc     1740

tccggcgtgg aagatcggtt caacgcctcc ctgggcacat accacgatct gctgaaaatt     1800

atcaaggaca aggacttcct ggacaatgag gaaaacgagg acattctgga agatatcgtg     1860

ctgaccctga cactgtttga ggacagagag atgatcgagg aacggctgaa aacctatgcc     1920

cacctgttcg acgacaaagt gatgaagcag ctgaagcggc ggagatacac cggctggggc     1980

aggctgagcc ggaagctgat caacggcatc cgggacaagc agtccggcaa gacaatcctg     2040

gatttcctga agtccgacgg cttcgccaac agaaacttca tgcagctgat ccacgacgac     2100

agcctgacct ttaaagagga catccagaaa gcccaggtgt ccggccaggg cgatagcctg     2160

cacgagcaca ttgccaatct ggccggcagc cccgccatta agaagggcat cctgcagaca     2220

gtgaaggtgg tggacgagct cgtgaaagtg atgggccggc acaagcccga gaacatcgtg     2280

atcgaaatgg ccagagagaa ccagaccacc cagaagggac agaagaacag ccgcgagaga     2340

atgaagcgga tcgaagaggg catcaaagag ctgggcagcc agatcctgaa agaacacccc     2400

gtggaaaaca cccagctgca gaacgagaag ctgtacctgt actacctgca gaatgggcgg     2460

gatatgtacg tggaccagga actggacatc aaccggctgt ccgactacga tgtggaccat     2520

atcgtgcctc agagctttct gaaggacgac tccatcgaca acaaggtgct gaccagaagc     2580

gacaagaacc ggggcaagag cgacaacgtg ccctccgaag aggtcgtgaa gaagatgaag     2640

aactactggc ggcagctgct gaacgccaag ctgattaccc agagaaagtt cgacaatctg     2700

accaaggccg agagaggcgg cctgagcgaa ctggataagg ccggcttcat caagagacag     2760

ctggtggaaa cccggcagat cacaaagcac gtggcacaga tcctggactc ccggatgaac     2820

actaagtacg acgagaatga caagctgatc cgggaagtga aagtgatcac cctgaagtcc     2880

aagctggtgt ccgatttccg gaaggatttc cagttttaca aagtgcgcga gatcaacaac     2940

taccaccacg cccacgacgc ctacctgaac gccgtcgtgg gaaccgccct gatcaaaaag     3000

taccctaagc tggaaagcga gttcgtgtac ggcgactaca aggtgtacga cgtgcggaag     3060

atgatcgcca agagcgagca ggaaatcggc aaggctaccg ccaagtactt cttctacagc     3120

aacatcatga actttttcaa gaccgagatt accctggcca acggcgagat ccggaagcgg     3180

cctctgatcg agacaaacgg cgaaaccggg gagatcgtgt gggataaggg ccgggatttt     3240

gccaccgtgc ggaaagtgct gagcatgccc caagtgaata tcgtgaaaaa gaccgaggtg     3300

cagacaggcg gcttcagcaa agagtctatc ctgcccaaga ggaacagcga taagctgatc     3360

gccagaaaga aggactggga ccctaagaag tacggcggct tcgacagccc caccgtggcc     3420

tattctgtgc tggtggtggc caaagtggaa aagggcaagt ccaagaaact gaagagtgtg     3480

aaagagctac tggggatcac catcatggaa agaagcagct tcgagaagaa tcccatcgac     3540

tttctggaag ccaagggcta caaagaagtg aaaaaggacc tgatcatcaa gctgcctaag     3600

tactccctgt tcgagctgga aaacggccgg aagagaatgc tggcctctgc cggcgaactg     3660

cagaagggaa acgaactggc cctgccctcc aaatatgtga acttcctgta cctggccagc     3720

cactatgaga agctgaaggg ctcccccgag gataatgagc agaaacagct gtttgtggaa     3780

cagcacaagc actacctgga cgagatcatc gagcagatca gcgagttctc caagagagtg     3840

atcctggccg acgctaatct ggacaaagtg ctgtccgcct acaacaagca ccgggataag     3900

cccatcagag agcaggccga gaatatcatc cacctgttta ccctgaccaa tctgggagcc     3960

cctgccgcct tcaagtactt tgacaccacc atcgaccgga agaggtacac cagcaccaaa     4020

gaggtgctgg acgccaccct gatccaccag agcatcaccg gcctgtacga gacacggatc     4080

gacctgtctc agctgggagg cgacaagcga cctgccgcca caaagaaggc tggacaggct     4140

aagaagaaga aagattacaa agacgatgac gataag                               4176


<210>  23
<211>  16
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  23
guuuuagagc uaugcu                                                       16


<210>  24
<211>  67
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  24
agcauagcaa guuaaaauaa ggcuaguccg uuaucaacuu gaaaaagugg caccgagucg       60

gugcuuu                                                                 67


<210>  25
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (2)..(21)
<223>  n = A, T, C, or G

<400>  25
gnnnnnnnnn nnnnnnnnnn ngg                                               23


<210>  26
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(21)
<223>  n = A, T, C, or G

<400>  26
nnnnnnnnnn nnnnnnnnnn ngg                                               23


<210>  27
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (3)..(23)
<223>  n = A, T, C, or G

<400>  27
ggnnnnnnnn nnnnnnnnnn nnngg                                             25


<210>  28
<211>  72
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  28
aaacagcaua gcaaguuaaa auaaggcuag uccguuauca acuugaaaaa guggcaccga       60

gucggugcuu uu                                                           72


<210>  29
<211>  82
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  29
guuggaacca uucaaaacag cauagcaagu uaaaauaagg cuaguccguu aucaacuuga       60

aaaaguggca ccgagucggu gc                                                82


<210>  30
<211>  83
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  30
guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc cguuaucaac uugaaaaagu       60

ggcaccgagu cggugcuuuu uuu                                               83


<210>  31
<211>  80
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  31
guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc cguuaucaac uugaaaaagu       60

ggcaccgagu cggugcuuuu                                                   80


<210>  32
<211>  92
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  32
guuuaagagc uaugcuggaa acagcauagc aaguuuaaau aaggcuaguc cguuaucaac       60

uugaaaaagu ggcaccgagu cggugcuuuu uu                                     92


<210>  33
<211>  34
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  33
ggccaacaug aggaucaccc augucugcag ggcc                                   34


<210>  34
<211>  137
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  34
guuuuagagc uaggccaaca ugaggaucac ccaugucugc agggccuagc aaguuaaaau       60

aaggcuaguc cguuaucaac uuggccaaca ugaggaucac ccaugucugc agggccaagu      120

ggcaccgagu cggugcu                                                     137


<210>  35
<211>  157
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  n is a, c, g, or u

<400>  35
nnnnnnnnnn nnnnnnnnnn guuuuagagc uaggccaaca ugaggaucac ccaugucugc       60

agggccuagc aaguuaaaau aaggcuaguc cguuaucaac uuggccaaca ugaggaucac      120

ccaugucugc agggccaagu ggcaccgagu cggugcu                               157


<210>  36
<211>  1471
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<222>  (1)..(1384)
<223>  dCas9

<220>
<221>  MISC_FEATURE
<222>  (1405)..(1409)
<223>  NLS

<220>
<221>  MISC_FEATURE
<222>  (1410)..(1471)
<223>  VP64

<400>  36

Met Lys Arg Pro Ala Ala Thr Lys Lys Ala Gly Gln Ala Lys Lys Lys 
1               5                   10                  15      


Lys Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly Thr Asn Ser Val 
            20                  25                  30          


Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe 
        35                  40                  45              


Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
    50                  55                  60                  


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
65                  70                  75                  80  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
                85                  90                  95      


Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
            100                 105                 110         


Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
        115                 120                 125             


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
    130                 135                 140                 


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp 
145                 150                 155                 160 


Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
                165                 170                 175     


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
            180                 185                 190         


Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
        195                 200                 205             


Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
    210                 215                 220                 


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
225                 230                 235                 240 


Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
                245                 250                 255     


Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
            260                 265                 270         


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
        275                 280                 285             


Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
    290                 295                 300                 


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
305                 310                 315                 320 


Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
                325                 330                 335     


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
            340                 345                 350         


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
        355                 360                 365             


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
    370                 375                 380                 


Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
385                 390                 395                 400 


Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg 
                405                 410                 415     


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
            420                 425                 430         


Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
        435                 440                 445             


Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
    450                 455                 460                 


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
465                 470                 475                 480 


Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
                485                 490                 495     


Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
            500                 505                 510         


Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
        515                 520                 525             


Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
    530                 535                 540                 


Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
545                 550                 555                 560 


Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
                565                 570                 575     


Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
            580                 585                 590         


Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
        595                 600                 605             


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
    610                 615                 620                 


Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
625                 630                 635                 640 


Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
                645                 650                 655     


His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
            660                 665                 670         


Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
        675                 680                 685             


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
    690                 695                 700                 


Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
705                 710                 715                 720 


Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
                725                 730                 735     


His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
            740                 745                 750         


Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
        755                 760                 765             


Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
    770                 775                 780                 


Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
785                 790                 795                 800 


Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro 
                805                 810                 815     


Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu 
            820                 825                 830         


Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg 
        835                 840                 845             


Leu Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Leu Lys 
    850                 855                 860                 


Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Ala Arg 
865                 870                 875                 880 


Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys 
                885                 890                 895     


Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys 
            900                 905                 910         


Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp 
        915                 920                 925             


Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
    930                 935                 940                 


Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp 
945                 950                 955                 960 


Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser 
                965                 970                 975     


Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg 
            980                 985                 990         


Glu Ile Asn Asn Tyr His His Ala  His Asp Ala Tyr Leu  Asn Ala Val 
        995                 1000                 1005             


Val Gly  Thr Ala Leu Ile Lys  Lys Tyr Pro Lys Leu  Glu Ser Glu 
    1010                 1015                 1020             


Phe Val  Tyr Gly Asp Tyr Lys  Val Tyr Asp Val Arg  Lys Met Ile 
    1025                 1030                 1035             


Ala Lys  Ser Glu Gln Glu Ile  Gly Lys Ala Thr Ala  Lys Tyr Phe 
    1040                 1045                 1050             


Phe Tyr  Ser Asn Ile Met Asn  Phe Phe Lys Thr Glu  Ile Thr Leu 
    1055                 1060                 1065             


Ala Asn  Gly Glu Ile Arg Lys  Arg Pro Leu Ile Glu  Thr Asn Gly 
    1070                 1075                 1080             


Glu Thr  Gly Glu Ile Val Trp  Asp Lys Gly Arg Asp  Phe Ala Thr 
    1085                 1090                 1095             


Val Arg  Lys Val Leu Ser Met  Pro Gln Val Asn Ile  Val Lys Lys 
    1100                 1105                 1110             


Thr Glu  Val Gln Thr Gly Gly  Phe Ser Lys Glu Ser  Ile Leu Pro 
    1115                 1120                 1125             


Lys Arg  Asn Ser Asp Lys Leu  Ile Ala Arg Lys Lys  Asp Trp Asp 
    1130                 1135                 1140             


Pro Lys  Lys Tyr Gly Gly Phe  Asp Ser Pro Thr Val  Ala Tyr Ser 
    1145                 1150                 1155             


Val Leu  Val Val Ala Lys Val  Glu Lys Gly Lys Ser  Lys Lys Leu 
    1160                 1165                 1170             


Lys Ser  Val Lys Glu Leu Leu  Gly Ile Thr Ile Met  Glu Arg Ser 
    1175                 1180                 1185             


Ser Phe  Glu Lys Asn Pro Ile  Asp Phe Leu Glu Ala  Lys Gly Tyr 
    1190                 1195                 1200             


Lys Glu  Val Lys Lys Asp Leu  Ile Ile Lys Leu Pro  Lys Tyr Ser 
    1205                 1210                 1215             


Leu Phe  Glu Leu Glu Asn Gly  Arg Lys Arg Met Leu  Ala Ser Ala 
    1220                 1225                 1230             


Gly Glu  Leu Gln Lys Gly Asn  Glu Leu Ala Leu Pro  Ser Lys Tyr 
    1235                 1240                 1245             


Val Asn  Phe Leu Tyr Leu Ala  Ser His Tyr Glu Lys  Leu Lys Gly 
    1250                 1255                 1260             


Ser Pro  Glu Asp Asn Glu Gln  Lys Gln Leu Phe Val  Glu Gln His 
    1265                 1270                 1275             


Lys His  Tyr Leu Asp Glu Ile  Ile Glu Gln Ile Ser  Glu Phe Ser 
    1280                 1285                 1290             


Lys Arg  Val Ile Leu Ala Asp  Ala Asn Leu Asp Lys  Val Leu Ser 
    1295                 1300                 1305             


Ala Tyr  Asn Lys His Arg Asp  Lys Pro Ile Arg Glu  Gln Ala Glu 
    1310                 1315                 1320             


Asn Ile  Ile His Leu Phe Thr  Leu Thr Asn Leu Gly  Ala Pro Ala 
    1325                 1330                 1335             


Ala Phe  Lys Tyr Phe Asp Thr  Thr Ile Asp Arg Lys  Arg Tyr Thr 
    1340                 1345                 1350             


Ser Thr  Lys Glu Val Leu Asp  Ala Thr Leu Ile His  Gln Ser Ile 
    1355                 1360                 1365             


Thr Gly  Leu Tyr Glu Thr Arg  Ile Asp Leu Ser Gln  Leu Gly Gly 
    1370                 1375                 1380             


Asp Ser  Ala Gly Gly Gly Gly  Ser Gly Gly Gly Gly  Ser Gly Gly 
    1385                 1390                 1395             


Gly Gly  Ser Gly Pro Lys Lys  Lys Arg Lys Val Ala  Ala Ala Gly 
    1400                 1405                 1410             


Ser Gly  Arg Ala Asp Ala Leu  Asp Asp Phe Asp Leu  Asp Met Leu 
    1415                 1420                 1425             


Gly Ser  Asp Ala Leu Asp Asp  Phe Asp Leu Asp Met  Leu Gly Ser 
    1430                 1435                 1440             


Asp Ala  Leu Asp Asp Phe Asp  Leu Asp Met Leu Gly  Ser Asp Ala 
    1445                 1450                 1455             


Leu Asp  Asp Phe Asp Leu Asp  Met Leu Ile Asn Cys  Thr 
    1460                 1465                 1470     


<210>  37
<211>  473
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<222>  (1)..(130)
<223>  MCP

<220>
<221>  MISC_FEATURE
<222>  (151)..(155)
<223>  NLS

<220>
<221>  MISC_FEATURE
<222>  (161)..(341)
<223>  P65

<220>
<221>  MISC_FEATURE
<222>  (350)..(473)
<223>  HSF1

<400>  37

Met Ala Ser Asn Phe Thr Gln Phe Val Leu Val Asp Asn Gly Gly Thr 
1               5                   10                  15      


Gly Asp Val Thr Val Ala Pro Ser Asn Phe Ala Asn Gly Val Ala Glu 
            20                  25                  30          


Trp Ile Ser Ser Asn Ser Arg Ser Gln Ala Tyr Lys Val Thr Cys Ser 
        35                  40                  45              


Val Arg Gln Ser Ser Ala Gln Lys Arg Lys Tyr Thr Ile Lys Val Glu 
    50                  55                  60                  


Val Pro Lys Val Ala Thr Gln Thr Val Gly Gly Val Glu Leu Pro Val 
65                  70                  75                  80  


Ala Ala Trp Arg Ser Tyr Leu Asn Met Glu Leu Thr Ile Pro Ile Phe 
                85                  90                  95      


Ala Thr Asn Ser Asp Cys Glu Leu Ile Val Lys Ala Met Gln Gly Leu 
            100                 105                 110         


Leu Lys Asp Gly Asn Pro Ile Pro Ser Ala Ile Ala Ala Asn Ser Gly 
        115                 120                 125             


Ile Tyr Ser Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
    130                 135                 140                 


Gly Gly Ser Gly Pro Lys Lys Lys Arg Lys Val Ala Ala Ala Gly Ser 
145                 150                 155                 160 


Pro Ser Gly Gln Ile Ser Asn Gln Ala Leu Ala Leu Ala Pro Ser Ser 
                165                 170                 175     


Ala Pro Val Leu Ala Gln Thr Met Val Pro Ser Ser Ala Met Val Pro 
            180                 185                 190         


Leu Ala Gln Pro Pro Ala Pro Ala Pro Val Leu Thr Pro Gly Pro Pro 
        195                 200                 205             


Gln Ser Leu Ser Ala Pro Val Pro Lys Ser Thr Gln Ala Gly Glu Gly 
    210                 215                 220                 


Thr Leu Ser Glu Ala Leu Leu His Leu Gln Phe Asp Ala Asp Glu Asp 
225                 230                 235                 240 


Leu Gly Ala Leu Leu Gly Asn Ser Thr Asp Pro Gly Val Phe Thr Asp 
                245                 250                 255     


Leu Ala Ser Val Asp Asn Ser Glu Phe Gln Gln Leu Leu Asn Gln Gly 
            260                 265                 270         


Val Ser Met Ser His Ser Thr Ala Glu Pro Met Leu Met Glu Tyr Pro 
        275                 280                 285             


Glu Ala Ile Thr Arg Leu Val Thr Gly Ser Gln Arg Pro Pro Asp Pro 
    290                 295                 300                 


Ala Pro Thr Pro Leu Gly Thr Ser Gly Leu Pro Asn Gly Leu Ser Gly 
305                 310                 315                 320 


Asp Glu Asp Phe Ser Ser Ile Ala Asp Met Asp Phe Ser Ala Leu Leu 
                325                 330                 335     


Ser Gln Ile Ser Ser Ser Gly Gln Gly Gly Gly Gly Ser Gly Phe Ser 
            340                 345                 350         


Val Asp Thr Ser Ala Leu Leu Asp Leu Phe Ser Pro Ser Val Thr Val 
        355                 360                 365             


Pro Asp Met Ser Leu Pro Asp Leu Asp Ser Ser Leu Ala Ser Ile Gln 
    370                 375                 380                 


Glu Leu Leu Ser Pro Gln Glu Pro Pro Arg Pro Pro Glu Ala Glu Asn 
385                 390                 395                 400 


Ser Ser Pro Asp Ser Gly Lys Gln Leu Val His Tyr Thr Ala Gln Pro 
                405                 410                 415     


Leu Phe Leu Leu Asp Pro Gly Ser Val Asp Thr Gly Ser Asn Asp Leu 
            420                 425                 430         


Pro Val Leu Phe Glu Leu Gly Glu Gly Ser Tyr Phe Ser Glu Gly Asp 
        435                 440                 445             


Gly Phe Ala Glu Asp Pro Thr Ile Ser Leu Leu Thr Gly Ser Glu Pro 
    450                 455                 460                 


Pro Lys Ala Lys Asp Pro Thr Val Ser 
465                 470             


<210>  38
<211>  4413
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(4152)
<223>  dCas9

<220>
<221>  misc_feature
<222>  (4213)..(4227)
<223>  NLS

<220>
<221>  misc_feature
<222>  (4228)..(4413)
<223>  VP64

<400>  38
atgaaaaggc cggcggccac gaaaaaggcc ggccaggcaa aaaagaaaaa ggacaagaag       60

tacagcatcg gcctggccat cggcaccaac tctgtgggct gggccgtgat caccgacgag      120

tacaaggtgc ccagcaagaa attcaaggtg ctgggcaaca ccgaccggca cagcatcaag      180

aagaacctga tcggagccct gctgttcgac agcggcgaaa cagccgaggc cacccggctg      240

aagagaaccg ccagaagaag atacaccaga cggaagaacc ggatctgcta tctgcaagag      300

atcttcagca acgagatggc caaggtggac gacagcttct tccacagact ggaagagtcc      360

ttcctggtgg aagaggataa gaagcacgag cggcacccca tcttcggcaa catcgtggac      420

gaggtggcct accacgagaa gtaccccacc atctaccacc tgagaaagaa actggtggac      480

agcaccgaca aggccgacct gcggctgatc tatctggccc tggcccacat gatcaagttc      540

cggggccact tcctgatcga gggcgacctg aaccccgaca acagcgacgt ggacaagctg      600

ttcatccagc tggtgcagac ctacaaccag ctgttcgagg aaaaccccat caacgccagc      660

ggcgtggacg ccaaggccat cctgtctgcc agactgagca agagcagacg gctggaaaat      720

ctgatcgccc agctgcccgg cgagaagaag aatggcctgt tcggcaacct gattgccctg      780

agcctgggcc tgacccccaa cttcaagagc aacttcgacc tggccgagga tgccaaactg      840

cagctgagca aggacaccta cgacgacgac ctggacaacc tgctggccca gatcggcgac      900

cagtacgccg acctgtttct ggccgccaag aacctgtccg acgccatcct gctgagcgac      960

atcctgagag tgaacaccga gatcaccaag gcccccctga gcgcctctat gatcaagaga     1020

tacgacgagc accaccagga cctgaccctg ctgaaagctc tcgtgcggca gcagctgcct     1080

gagaagtaca aagagatttt cttcgaccag agcaagaacg gctacgccgg ctacattgac     1140

ggcggagcca gccaggaaga gttctacaag ttcatcaagc ccatcctgga aaagatggac     1200

ggcaccgagg aactgctcgt gaagctgaac agagaggacc tgctgcggaa gcagcggacc     1260

ttcgacaacg gcagcatccc ccaccagatc cacctgggag agctgcacgc cattctgcgg     1320

cggcaggaag atttttaccc attcctgaag gacaaccggg aaaagatcga gaagatcctg     1380

accttccgca tcccctacta cgtgggccct ctggccaggg gaaacagcag attcgcctgg     1440

atgaccagaa agagcgagga aaccatcacc ccctggaact tcgaggaagt ggtggacaag     1500

ggcgcttccg cccagagctt catcgagcgg atgaccaact tcgataagaa cctgcccaac     1560

gagaaggtgc tgcccaagca cagcctgctg tacgagtact tcaccgtgta taacgagctg     1620

accaaagtga aatacgtgac cgagggaatg agaaagcccg ccttcctgag cggcgagcag     1680

aaaaaggcca tcgtggacct gctgttcaag accaaccgga aagtgaccgt gaagcagctg     1740

aaagaggact acttcaagaa aatcgagtgc ttcgactccg tggaaatctc cggcgtggaa     1800

gatcggttca acgcctccct gggcacatac cacgatctgc tgaaaattat caaggacaag     1860

gacttcctgg acaatgagga aaacgaggac attctggaag atatcgtgct gaccctgaca     1920

ctgtttgagg acagagagat gatcgaggaa cggctgaaaa cctatgccca cctgttcgac     1980

gacaaagtga tgaagcagct gaagcggcgg agatacaccg gctggggcag gctgagccgg     2040

aagctgatca acggcatccg ggacaagcag tccggcaaga caatcctgga tttcctgaag     2100

tccgacggct tcgccaacag aaacttcatg cagctgatcc acgacgacag cctgaccttt     2160

aaagaggaca tccagaaagc ccaggtgtcc ggccagggcg atagcctgca cgagcacatt     2220

gccaatctgg ccggcagccc cgccattaag aagggcatcc tgcagacagt gaaggtggtg     2280

gacgagctcg tgaaagtgat gggccggcac aagcccgaga acatcgtgat cgaaatggcc     2340

agagagaacc agaccaccca gaagggacag aagaacagcc gcgagagaat gaagcggatc     2400

gaagagggca tcaaagagct gggcagccag atcctgaaag aacaccccgt ggaaaacacc     2460

cagctgcaga acgagaagct gtacctgtac tacctgcaga atgggcggga tatgtacgtg     2520

gaccaggaac tggacatcaa ccggctgtcc gactacgatg tggaccacat cgtgcctcag     2580

agctttctga aggacgactc catcgacaac aaggtgctga ccagaagcga caaggcccgg     2640

ggcaagagcg acaacgtgcc ctccgaagag gtcgtgaaga agatgaagaa ctactggcgg     2700

cagctgctga acgccaagct gattacccag agaaagttcg acaatctgac caaggccgag     2760

agaggcggcc tgagcgaact ggataaggcc ggcttcatca agagacagct ggtggaaacc     2820

cggcagatca caaagcacgt ggcacagatc ctggactccc ggatgaacac taagtacgac     2880

gagaatgaca agctgatccg ggaagtgaaa gtgatcaccc tgaagtccaa gctggtgtcc     2940

gatttccgga aggatttcca gttttacaaa gtgcgcgaga tcaacaacta ccaccacgcc     3000

cacgacgcct acctgaacgc cgtcgtggga accgccctga tcaaaaagta ccctaagctg     3060

gaaagcgagt tcgtgtacgg cgactacaag gtgtacgacg tgcggaagat gatcgccaag     3120

agcgagcagg aaatcggcaa ggctaccgcc aagtacttct tctacagcaa catcatgaac     3180

tttttcaaga ccgagattac cctggccaac ggcgagatcc ggaagcggcc tctgatcgag     3240

acaaacggcg aaaccgggga gatcgtgtgg gataagggcc gggattttgc caccgtgcgg     3300

aaagtgctga gcatgcccca agtgaatatc gtgaaaaaga ccgaggtgca gacaggcggc     3360

ttcagcaaag agtctatcct gcccaagagg aacagcgata agctgatcgc cagaaagaag     3420

gactgggacc ctaagaagta cggcggcttc gacagcccca ccgtggccta ttctgtgctg     3480

gtggtggcca aagtggaaaa gggcaagtcc aagaaactga agagtgtgaa agagctgctg     3540

gggatcacca tcatggaaag aagcagcttc gagaagaatc ccatcgactt tctggaagcc     3600

aagggctaca aagaagtgaa aaaggacctg atcatcaagc tgcctaagta ctccctgttc     3660

gagctggaaa acggccggaa gagaatgctg gcctctgccg gcgaactgca gaagggaaac     3720

gaactggccc tgccctccaa atatgtgaac ttcctgtacc tggccagcca ctatgagaag     3780

ctgaagggct cccccgagga taatgagcag aaacagctgt ttgtggaaca gcacaagcac     3840

tacctggacg agatcatcga gcagatcagc gagttctcca agagagtgat cctggccgac     3900

gctaatctgg acaaagtgct gtccgcctac aacaagcacc gggataagcc catcagagag     3960

caggccgaga atatcatcca cctgtttacc ctgaccaatc tgggagcccc tgccgccttc     4020

aagtactttg acaccaccat cgaccggaag aggtacacca gcaccaaaga ggtgctggac     4080

gccaccctga tccaccagag catcaccggc ctgtacgaga cacggatcga cctgtctcag     4140

ctgggaggcg acagcgctgg aggaggtgga agcggaggag gaggaagcgg aggaggaggt     4200

agcggaccta agaaaaagag gaaggtggcg gccgctggat ccggacgggc tgacgcattg     4260

gacgattttg atctggatat gctgggaagt gacgccctcg atgattttga ccttgacatg     4320

cttggttcgg atgcccttga tgactttgac ctcgacatgc tcggcagtga cgcccttgat     4380

gatttcgacc tggacatgct gattaactgt aca                                  4413


<210>  39
<211>  1419
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(390)
<223>  MCP

<220>
<221>  misc_feature
<222>  (451)..(465)
<223>  NLS

<220>
<221>  misc_feature
<222>  (481)..(1023)
<223>  P65

<220>
<221>  misc_feature
<222>  (1048)..(1419)
<223>  HSF1

<400>  39
atggcttcaa actttactca gttcgtgctc gtggacaatg gtgggacagg ggatgtgaca       60

gtggctcctt ctaatttcgc taatggggtg gcagagtgga tcagctccaa ctcacggagc      120

caggcctaca aggtgacatg cagcgtcagg cagtctagtg cccagaagag aaagtatacc      180

atcaaggtgg aggtccccaa agtggctacc cagacagtgg gcggagtcga actgcctgtc      240

gccgcttgga ggtcctacct gaacatggag ctcactatcc caattttcgc taccaattct      300

gactgtgaac tcatcgtgaa ggcaatgcag gggctcctca aagacggtaa tcctatccct      360

tccgccatcg ccgctaactc aggtatctac agcgctggag gaggtggaag cggaggagga      420

ggaagcggag gaggaggtag cggacctaag aaaaagagga aggtggcggc cgctggatcc      480

ccttcagggc agatcagcaa ccaggccctg gctctggccc ctagctccgc tccagtgctg      540

gcccagacta tggtgccctc tagtgctatg gtgcctctgg cccagccacc tgctccagcc      600

cctgtgctga ccccaggacc accccagtca ctgagcgctc cagtgcccaa gtctacacag      660

gccggcgagg ggactctgag tgaagctctg ctgcacctgc agttcgacgc tgatgaggac      720

ctgggagctc tgctggggaa cagcaccgat cccggagtgt tcacagatct ggcctccgtg      780

gacaactctg agtttcagca gctgctgaat cagggcgtgt ccatgtctca tagtacagcc      840

gaaccaatgc tgatggagta ccccgaagcc attacccggc tggtgaccgg cagccagcgg      900

ccccccgacc ccgctccaac tcccctggga accagcggcc tgcctaatgg gctgtccgga      960

gatgaagact tctcaagcat cgctgatatg gactttagtg ccctgctgtc acagatttcc     1020

tctagtgggc agggaggagg tggaagcggc ttcagcgtgg acaccagtgc cctgctggac     1080

ctgttcagcc cctcggtgac cgtgcccgac atgagcctgc ctgaccttga cagcagcctg     1140

gccagtatcc aagagctcct gtctccccag gagcccccca ggcctcccga ggcagagaac     1200

agcagcccgg attcagggaa gcagctggtg cactacacag cgcagccgct gttcctgctg     1260

gaccccggct ccgtggacac cgggagcaac gacctgccgg tgctgtttga gctgggagag     1320

ggctcctact tctccgaagg ggacggcttc gccgaggacc ccaccatctc cctgctgaca     1380

ggctcggagc ctcccaaagc caaggacccc actgtctcc                            1419


<210>  40
<211>  130
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  40

Met Ala Ser Asn Phe Thr Gln Phe Val Leu Val Asp Asn Gly Gly Thr 
1               5                   10                  15      


Gly Asp Val Thr Val Ala Pro Ser Asn Phe Ala Asn Gly Val Ala Glu 
            20                  25                  30          


Trp Ile Ser Ser Asn Ser Arg Ser Gln Ala Tyr Lys Val Thr Cys Ser 
        35                  40                  45              


Val Arg Gln Ser Ser Ala Gln Lys Arg Lys Tyr Thr Ile Lys Val Glu 
    50                  55                  60                  


Val Pro Lys Val Ala Thr Gln Thr Val Gly Gly Val Glu Leu Pro Val 
65                  70                  75                  80  


Ala Ala Trp Arg Ser Tyr Leu Asn Met Glu Leu Thr Ile Pro Ile Phe 
                85                  90                  95      


Ala Thr Asn Ser Asp Cys Glu Leu Ile Val Lys Ala Met Gln Gly Leu 
            100                 105                 110         


Leu Lys Asp Gly Asn Pro Ile Pro Ser Ala Ile Ala Ala Asn Ser Gly 
        115                 120                 125             


Ile Tyr 
    130 


<210>  41
<211>  390
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  41
atggcttcaa actttactca gttcgtgctc gtggacaatg gtgggacagg ggatgtgaca       60

gtggctcctt ctaatttcgc taatggggtg gcagagtgga tcagctccaa ctcacggagc      120

caggcctaca aggtgacatg cagcgtcagg cagtctagtg cccagaagag aaagtatacc      180

atcaaggtgg aggtccccaa agtggctacc cagacagtgg gcggagtcga actgcctgtc      240

gccgcttgga ggtcctacct gaacatggag ctcactatcc caattttcgc taccaattct      300

gactgtgaac tcatcgtgaa ggcaatgcag gggctcctca aagacggtaa tcctatccct      360

tccgccatcg ccgctaactc aggtatctac                                       390


<210>  42
<211>  6673
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  42
gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg       60

tcggcaattg aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg      120

tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg      180

ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg      240

gttcccgcgg gcctggcctc tttacgggtt atggcccttg cgtgccttga attacttcca      300

cctggctgca gtacgtgatt cttgatcccg agcttcgggt tggaagtggg tgggagagtt      360

cgaggccttg cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc      420

gctggggccg ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct gctttcgata      480

agtctctagc catttaaaat ttttgatgac ctgctgcgac gctttttttc tggcaagata      540

gtcttgtaaa tgcgggccaa gatctgcaca ctggtatttc ggtttttggg gccgcgggcg      600

gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg cgagcgcggc      660

caccgagaat cggacggggg tagtctcaag ctggccggcc tgctctggtg cctggcctcg      720

cgccgccgtg tatcgccccg ccctgggcgg caaggctggc ccggtcggca ccagttgcgt      780

gagcggaaag atggccgctt cccggccctg ctgcagggag ctcaaaatgg aggacgcggc      840

gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa aagggccttt ccgtcctcag      900

ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc caggcacctc gattagttct      960

cgagcttttg gagtacgtcg tctttaggtt ggggggaggg gttttatgcg atggagtttc     1020

cccacactga gtgggtggag actgaagtta ggccagcttg gcacttgatg taattctcct     1080

tggaatttgc cctttttgag tttggatctt ggttcattct caagcctcag acagtggttc     1140

aaagtttttt tcttccattt caggtgtcgt gacgtacggc caccatgaaa aggccggcgg     1200

ccacgaaaaa ggccggccag gcaaaaaaga aaaaggacaa gaagtacagc atcggcctgg     1260

ccatcggcac caactctgtg ggctgggccg tgatcaccga cgagtacaag gtgcccagca     1320

agaaattcaa ggtgctgggc aacaccgacc ggcacagcat caagaagaac ctgatcggag     1380

ccctgctgtt cgacagcggc gaaacagccg aggccacccg gctgaagaga accgccagaa     1440

gaagatacac cagacggaag aaccggatct gctatctgca agagatcttc agcaacgaga     1500

tggccaaggt ggacgacagc ttcttccaca gactggaaga gtccttcctg gtggaagagg     1560

ataagaagca cgagcggcac cccatcttcg gcaacatcgt ggacgaggtg gcctaccacg     1620

agaagtaccc caccatctac cacctgagaa agaaactggt ggacagcacc gacaaggccg     1680

acctgcggct gatctatctg gccctggccc acatgatcaa gttccggggc cacttcctga     1740

tcgagggcga cctgaacccc gacaacagcg acgtggacaa gctgttcatc cagctggtgc     1800

agacctacaa ccagctgttc gaggaaaacc ccatcaacgc cagcggcgtg gacgccaagg     1860

ccatcctgtc tgccagactg agcaagagca gacggctgga aaatctgatc gcccagctgc     1920

ccggcgagaa gaagaatggc ctgttcggca acctgattgc cctgagcctg ggcctgaccc     1980

ccaacttcaa gagcaacttc gacctggccg aggatgccaa actgcagctg agcaaggaca     2040

cctacgacga cgacctggac aacctgctgg cccagatcgg cgaccagtac gccgacctgt     2100

ttctggccgc caagaacctg tccgacgcca tcctgctgag cgacatcctg agagtgaaca     2160

ccgagatcac caaggccccc ctgagcgcct ctatgatcaa gagatacgac gagcaccacc     2220

aggacctgac cctgctgaaa gctctcgtgc ggcagcagct gcctgagaag tacaaagaga     2280

ttttcttcga ccagagcaag aacggctacg ccggctacat tgacggcgga gccagccagg     2340

aagagttcta caagttcatc aagcccatcc tggaaaagat ggacggcacc gaggaactgc     2400

tcgtgaagct gaacagagag gacctgctgc ggaagcagcg gaccttcgac aacggcagca     2460

tcccccacca gatccacctg ggagagctgc acgccattct gcggcggcag gaagattttt     2520

acccattcct gaaggacaac cgggaaaaga tcgagaagat cctgaccttc cgcatcccct     2580

actacgtggg ccctctggcc aggggaaaca gcagattcgc ctggatgacc agaaagagcg     2640

aggaaaccat caccccctgg aacttcgagg aagtggtgga caagggcgct tccgcccaga     2700

gcttcatcga gcggatgacc aacttcgata agaacctgcc caacgagaag gtgctgccca     2760

agcacagcct gctgtacgag tacttcaccg tgtataacga gctgaccaaa gtgaaatacg     2820

tgaccgaggg aatgagaaag cccgccttcc tgagcggcga gcagaaaaag gccatcgtgg     2880

acctgctgtt caagaccaac cggaaagtga ccgtgaagca gctgaaagag gactacttca     2940

agaaaatcga gtgcttcgac tccgtggaaa tctccggcgt ggaagatcgg ttcaacgcct     3000

ccctgggcac ataccacgat ctgctgaaaa ttatcaagga caaggacttc ctggacaatg     3060

aggaaaacga ggacattctg gaagatatcg tgctgaccct gacactgttt gaggacagag     3120

agatgatcga ggaacggctg aaaacctatg cccacctgtt cgacgacaaa gtgatgaagc     3180

agctgaagcg gcggagatac accggctggg gcaggctgag ccggaagctg atcaacggca     3240

tccgggacaa gcagtccggc aagacaatcc tggatttcct gaagtccgac ggcttcgcca     3300

acagaaactt catgcagctg atccacgacg acagcctgac ctttaaagag gacatccaga     3360

aagcccaggt gtccggccag ggcgatagcc tgcacgagca cattgccaat ctggccggca     3420

gccccgccat taagaagggc atcctgcaga cagtgaaggt ggtggacgag ctcgtgaaag     3480

tgatgggccg gcacaagccc gagaacatcg tgatcgaaat ggccagagag aaccagacca     3540

cccagaaggg acagaagaac agccgcgaga gaatgaagcg gatcgaagag ggcatcaaag     3600

agctgggcag ccagatcctg aaagaacacc ccgtggaaaa cacccagctg cagaacgaga     3660

agctgtacct gtactacctg cagaatgggc gggatatgta cgtggaccag gaactggaca     3720

tcaaccggct gtccgactac gatgtggacc acatcgtgcc tcagagcttt ctgaaggacg     3780

actccatcga caacaaggtg ctgaccagaa gcgacaaggc ccggggcaag agcgacaacg     3840

tgccctccga agaggtcgtg aagaagatga agaactactg gcggcagctg ctgaacgcca     3900

agctgattac ccagagaaag ttcgacaatc tgaccaaggc cgagagaggc ggcctgagcg     3960

aactggataa ggccggcttc atcaagagac agctggtgga aacccggcag atcacaaagc     4020

acgtggcaca gatcctggac tcccggatga acactaagta cgacgagaat gacaagctga     4080

tccgggaagt gaaagtgatc accctgaagt ccaagctggt gtccgatttc cggaaggatt     4140

tccagtttta caaagtgcgc gagatcaaca actaccacca cgcccacgac gcctacctga     4200

acgccgtcgt gggaaccgcc ctgatcaaaa agtaccctaa gctggaaagc gagttcgtgt     4260

acggcgacta caaggtgtac gacgtgcgga agatgatcgc caagagcgag caggaaatcg     4320

gcaaggctac cgccaagtac ttcttctaca gcaacatcat gaactttttc aagaccgaga     4380

ttaccctggc caacggcgag atccggaagc ggcctctgat cgagacaaac ggcgaaaccg     4440

gggagatcgt gtgggataag ggccgggatt ttgccaccgt gcggaaagtg ctgagcatgc     4500

cccaagtgaa tatcgtgaaa aagaccgagg tgcagacagg cggcttcagc aaagagtcta     4560

tcctgcccaa gaggaacagc gataagctga tcgccagaaa gaaggactgg gaccctaaga     4620

agtacggcgg cttcgacagc cccaccgtgg cctattctgt gctggtggtg gccaaagtgg     4680

aaaagggcaa gtccaagaaa ctgaagagtg tgaaagagct gctggggatc accatcatgg     4740

aaagaagcag cttcgagaag aatcccatcg actttctgga agccaagggc tacaaagaag     4800

tgaaaaagga cctgatcatc aagctgccta agtactccct gttcgagctg gaaaacggcc     4860

ggaagagaat gctggcctct gccggcgaac tgcagaaggg aaacgaactg gccctgccct     4920

ccaaatatgt gaacttcctg tacctggcca gccactatga gaagctgaag ggctcccccg     4980

aggataatga gcagaaacag ctgtttgtgg aacagcacaa gcactacctg gacgagatca     5040

tcgagcagat cagcgagttc tccaagagag tgatcctggc cgacgctaat ctggacaaag     5100

tgctgtccgc ctacaacaag caccgggata agcccatcag agagcaggcc gagaatatca     5160

tccacctgtt taccctgacc aatctgggag cccctgccgc cttcaagtac tttgacacca     5220

ccatcgaccg gaagaggtac accagcacca aagaggtgct ggacgccacc ctgatccacc     5280

agagcatcac cggcctgtac gagacacgga tcgacctgtc tcagctggga ggcgacagcg     5340

ctggaggagg tggaagcgga ggaggaggaa gcggaggagg aggtagcgga cctaagaaaa     5400

agaggaaggt ggcggccgct ggatccggac gggctgacgc attggacgat tttgatctgg     5460

atatgctggg aagtgacgcc ctcgatgatt ttgaccttga catgcttggt tcggatgccc     5520

ttgatgactt tgacctcgac atgctcggca gtgacgccct tgatgatttc gacctggaca     5580

tgctgattaa ctgtacaggc agtggagagg gcagaggaag tctgctaaca tgcggtgacg     5640

tcgaggagaa tcctggccca atggccaagc ctttgtctca agaagaatcc accctcattg     5700

aaagagcaac ggctacaatc aacagcatcc ccatctctga agactacagc gtcgccagcg     5760

cagctctctc tagcgacggc cgcatcttca ctggtgtcaa tgtatatcat tttactgggg     5820

gaccttgtgc agaactcgtg gtgctgggca ctgctgctgc tgcggcagct ggcaacctga     5880

cttgtatcgt cgcgatcgga aatgagaaca ggggcatctt gagcccctgc ggacggtgcc     5940

gacaggtgct tctcgatctg catcctggga tcaaagccat agtgaaggac agtgatggac     6000

agccgacggc agttgggatt cgtgaattgc tgccctctgg ttatgtgtgg gagggctaag     6060

aattcgatat caagcttatc ggtaatcaac ctctggatta caaaatttgt gaaagattga     6120

ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt     6180

tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt     6240

tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg     6300

tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg     6360

ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc     6420

gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat     6480

catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct     6540

tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg     6600

ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg     6660

ccgcctcccc gca                                                        6673


<210>  43
<211>  4324
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  43
gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg       60

tcggcaattg aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg      120

tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg      180

ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg      240

gttcccgcgg gcctggcctc tttacgggtt atggcccttg cgtgccttga attacttcca      300

cctggctgca gtacgtgatt cttgatcccg agcttcgggt tggaagtggg tgggagagtt      360

cgaggccttg cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc      420

gctggggccg ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct gctttcgata      480

agtctctagc catttaaaat ttttgatgac ctgctgcgac gctttttttc tggcaagata      540

gtcttgtaaa tgcgggccaa gatctgcaca ctggtatttc ggtttttggg gccgcgggcg      600

gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg cgagcgcggc      660

caccgagaat cggacggggg tagtctcaag ctggccggcc tgctctggtg cctggcctcg      720

cgccgccgtg tatcgccccg ccctgggcgg caaggctggc ccggtcggca ccagttgcgt      780

gagcggaaag atggccgctt cccggccctg ctgcagggag ctcaaaatgg aggacgcggc      840

gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa aagggccttt ccgtcctcag      900

ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc caggcacctc gattagttct      960

cgagcttttg gagtacgtcg tctttaggtt ggggggaggg gttttatgcg atggagtttc     1020

cccacactga gtgggtggag actgaagtta ggccagcttg gcacttgatg taattctcct     1080

tggaatttgc cctttttgag tttggatctt ggttcattct caagcctcag acagtggttc     1140

aaagtttttt tcttccattt caggtgtcgt gacgtacggc caccatggct tcaaacttta     1200

ctcagttcgt gctcgtggac aatggtggga caggggatgt gacagtggct ccttctaatt     1260

tcgctaatgg ggtggcagag tggatcagct ccaactcacg gagccaggcc tacaaggtga     1320

catgcagcgt caggcagtct agtgcccaga agagaaagta taccatcaag gtggaggtcc     1380

ccaaagtggc tacccagaca gtgggcggag tcgaactgcc tgtcgccgct tggaggtcct     1440

acctgaacat ggagctcact atcccaattt tcgctaccaa ttctgactgt gaactcatcg     1500

tgaaggcaat gcaggggctc ctcaaagacg gtaatcctat cccttccgcc atcgccgcta     1560

actcaggtat ctacagcgct ggaggaggtg gaagcggagg aggaggaagc ggaggaggag     1620

gtagcggacc taagaaaaag aggaaggtgg cggccgctgg atccccttca gggcagatca     1680

gcaaccaggc cctggctctg gcccctagct ccgctccagt gctggcccag actatggtgc     1740

cctctagtgc tatggtgcct ctggcccagc cacctgctcc agcccctgtg ctgaccccag     1800

gaccacccca gtcactgagc gctccagtgc ccaagtctac acaggccggc gaggggactc     1860

tgagtgaagc tctgctgcac ctgcagttcg acgctgatga ggacctggga gctctgctgg     1920

ggaacagcac cgatcccgga gtgttcacag atctggcctc cgtggacaac tctgagtttc     1980

agcagctgct gaatcagggc gtgtccatgt ctcatagtac agccgaacca atgctgatgg     2040

agtaccccga agccattacc cggctggtga ccggcagcca gcggcccccc gaccccgctc     2100

caactcccct gggaaccagc ggcctgccta atgggctgtc cggagatgaa gacttctcaa     2160

gcatcgctga tatggacttt agtgccctgc tgtcacagat ttcctctagt gggcagggag     2220

gaggtggaag cggcttcagc gtggacacca gtgccctgct ggacctgttc agcccctcgg     2280

tgaccgtgcc cgacatgagc ctgcctgacc ttgacagcag cctggccagt atccaagagc     2340

tcctgtctcc ccaggagccc cccaggcctc ccgaggcaga gaacagcagc ccggattcag     2400

ggaagcagct ggtgcactac acagcgcagc cgctgttcct gctggacccc ggctccgtgg     2460

acaccgggag caacgacctg ccggtgctgt ttgagctggg agagggctcc tacttctccg     2520

aaggggacgg cttcgccgag gaccccacca tctccctgct gacaggctcg gagcctccca     2580

aagccaagga ccccactgtc tcctgtacag gcagtggaga gggcagagga agtctgctaa     2640

catgcggtga cgtcgaggag aatcctggcc caaccatgaa aaagcctgaa ctcaccgcta     2700

cctctgtcga gaagtttctg atcgaaaagt tcgacagcgt ctccgacctg atgcagctct     2760

ccgagggcga agaatctcgg gctttcagct tcgatgtggg agggcgtgga tatgtcctgc     2820

gggtgaatag ctgcgccgat ggtttctaca aagatcgcta tgtttatcgg cactttgcat     2880

ccgccgctct ccctattccc gaagtgcttg acattgggga gttcagcgag agcctgacct     2940

attgcatctc ccgccgtgca cagggtgtca ccttgcaaga cctgcctgaa accgaactgc     3000

ccgctgttct ccagcccgtc gccgaggcca tggatgccat cgctgccgcc gatcttagcc     3060

agaccagcgg gttcggccca ttcggacctc aaggaatcgg tcaatacact acatggcgcg     3120

atttcatctg cgctattgct gatccccatg tgtatcactg gcaaactgtg atggacgaca     3180

ccgtcagtgc ctccgtcgcc caggctctcg atgagctgat gctttgggcc gaggactgcc     3240

ccgaagtccg gcacctcgtg cacgccgatt tcggctccaa caatgtcctg accgacaatg     3300

gccgcataac agccgtcatt gactggagcg aggccatgtt cggggattcc caatacgagg     3360

tcgccaacat cttcttctgg aggccctggt tggcttgtat ggagcagcag acccgctact     3420

tcgagcggag gcatcccgag cttgcaggat ctcctcggct ccgggcttat atgctccgca     3480

ttggtcttga ccaactctat cagagcttgg ttgacggcaa tttcgatgat gcagcttggg     3540

ctcagggtcg ctgcgacgca atcgtccggt ccggagccgg gactgtcggg cgtacacaaa     3600

tcgcccgcag aagcgctgcc gtctggaccg atggctgtgt ggaagtgctc gccgatagtg     3660

gaaacagacg ccccagcact cgtcctaggg caaaggatct gcagtaatga gaattcgata     3720

tcaagcttat cggtaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc     3780

ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg     3840

ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc     3900

tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg     3960

acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg     4020

ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga     4080

caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct     4140

ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg     4200

tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc     4260

ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc     4320

cgca                                                                  4324


