                         SEQUENCE LISTING

<110>  Teva Pharmaceuticals
 
<120>  IMPROVED PROCESSES FOR THE PREPARATION OF SEMAGLUTIDE

<130>  2873.301PC02

<150>  US 62/819,826
<151>  2019-03-18

<150>  US 62/819,031
<151>  2019-03-15

<160>  51    

<170>  PatentIn version 3.5

<210>  1
<211>  31
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Peptide fragment


<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Wherein Xaa is Aib

<220>
<221>  misc_feature
<222>  (20)..(20)
<223>  wherein Lys is Lys- 
       N-(17-carboxy-1-oxoheptadecyl)-L-gamma-glutamyl-2-[2-(2-aminoethoxy)e
       thoxy]acetyl-2-[2-(2-aminoethoxy)ethoxy]acetyl

<400>  1

His Xaa Glu Gly Thr Phe Thr Ser Asp Val Ser Ser Tyr Leu Glu Gly 
1               5                   10                  15      


Gln Ala Ala Lys Glu Phe Ile Ala Trp Leu Val Arg Gly Arg Gly 
            20                  25                  30      


<210>  2
<211>  31
<212>  PRT
<213>  artificial sequence

<220>
<223>  Peptide fragment


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  wherein Xaa is His, D-His, desamino-histidine, 2-amino-histidine,
       beta-hydroxy-histidine, homohistidine, 
       alpha-fluoromethyl-histidine or alpha-methyl-histidine

<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Xaa has side chain H-, Ac-, Boc-, or Fmoc-

<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Xaa may occur either one or zero times

<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Xaa is -Ala, D-Ala, -Val, D-Val, Gly, Aib (alpha-aminoisobutyric 
       acid)

<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Xaa may occur one or zero times, but will always occur once if 
       the first residue occurs once

<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  Xaa can be Glu, Asp, Met, Leu, but is preferably Glu or Asp, and 
       is most preferably Asp

<220>
<221>  misc_feature
<222>  (16)..(16)
<223>  Xaa is Gly or Aib

<220>
<221>  misc_feature
<222>  (28)..(28)
<223>  Xaa is Lys or Arg

<220>
<221>  misc_feature
<222>  (29)..(29)
<223>  Xaa is Gly, Aib, Ala, D-Ala

<220>
<221>  misc_feature
<222>  (30)..(30)
<223>  Xaa is Arg, Lys or Gly

<220>
<221>  misc_feature
<222>  (30)..(30)
<223>  Xaa may occur one or zero times, but will always occur once if 
       the 31st residue occurs once

<220>
<221>  misc_feature
<222>  (31)..(31)
<223>  Xaa can be Arg, Lys or Gly but is preferably Lys or Gly

<220>
<221>  misc_feature
<222>  (31)..(31)
<223>  Xaa may occur either one or zero times

<220>
<221>  misc_feature
<222>  (31)..(31)
<223>  Xaa has side chain -OH or -NH2

<400>  2

Xaa Xaa Xaa Gly Thr Phe Thr Ser Asp Val Ser Ser Tyr Leu Glu Xaa 
1               5                   10                  15      


Gln Ala Ala Lys Glu Phe Ile Ala Trp Leu Val Xaa Xaa Xaa Xaa 
            20                  25                  30      


<210>  3
<211>  17
<212>  PRT
<213>  artificial sequence

<220>
<223>  Peptide fragment


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  N-terminus of His is optionally protected with a protecting 
       group; in some embodiments, the protecting group is selected from
       the group consisting of Boc, Cbz or Fmoc,

<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Xaa is Aib

<220>
<221>  misc_feature
<222>  (17)..(17)
<223>  the Gly carboxylic acid group in Peptide 1 may be in the form of 
       an activated carboxylic acid derivative;

<400>  3

His Xaa Glu Glu Gly Thr Phe Thr Ser Asp Val Ser Ser Tyr Leu Glu 
1               5                   10                  15      


Gly 
    


<210>  4
<211>  15
<212>  PRT
<213>  artificial sequence

<220>
<223>  Peptide fragment


<220>
<221>  Misc_Feature
<222>  (4)..(4)
<223>  Wherein W1 = 
       N-(17-carboxy-1-oxoheptadecyl)-L-gamma-glutamyl-2-[2-(2-aminoethoxy)e
       thoxy]acetyl-2-[2-(2-aminoethoxy)ethoxy]acetyl

<400>  4

Gln Ala Ala Lys Glu Phe Ile Ala Trp Leu Val Arg Gly Arg Gly 
1               5                   10                  15  


<210>  5
<211>  15
<212>  PRT
<213>  artificial sequence

<220>
<223>  Peptide fragment


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Xaa is Aib

<400>  5

Xaa Glu Gly Thr Phe Thr Ser Asp Val Ser Ser Tyr Leu Glu Gly 
1               5                   10                  15  


<210>  6
<211>  30
<212>  PRT
<213>  artificial sequence

<220>
<223>  Peptide fragment


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Xaa is Aib

<220>
<221>  misc_feature
<222>  (19)..(19)
<223>  wherein Lys is Lys- 
       N-(17-carboxy-1-oxoheptadecyl)-L-gamma-glutamyl-2-[2-(2-aminoethoxy)e
       thoxy]acetyl-2-[2-(2-aminoethoxy)ethoxy]acetyl

<400>  6

Xaa Glu Gly Thr Phe Thr Ser Asp Val Ser Ser Tyr Leu Glu Gly Gln 
1               5                   10                  15      


Ala Ala Lys Glu Phe Ile Ala Trp Leu Val Arg Gly Arg Gly 
            20                  25                  30  


<210>  7
<211>  32
<212>  PRT
<213>  artificial sequence

<220>
<223>  Peptide fragment


<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Xaa is Aib

<220>
<221>  misc_feature
<222>  (21)..(21)
<223>  Lys(N-(17-carboxy-1-oxoheptadecyl)-L-gamma-glutamyl-2-[2-(2-amino
       ethoxy)ethoxy]acetyl-2-[2-(2-aminoethoxy)ethoxy]acetyl)

<400>  7

His Xaa Glu Gly Gly Thr Phe Thr Ser Asp Val Ser Ser Tyr Leu Glu 
1               5                   10                  15      


Gly Gln Ala Ala Lys Glu Phe Ile Ala Trp Leu Val Arg Gly Arg Gly 
            20                  25                  30          


<210>  8
<211>  32
<212>  PRT
<213>  artificial sequence

<220>
<223>  Peptide fragment


<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Xaa is Aib

<220>
<221>  misc_feature
<222>  (21)..(21)
<223>  wherein Lys is Lys- 
       N-(17-carboxy-1-oxoheptadecyl)-L-gamma-glutamyl-2-[2-(2-aminoethoxy)e
       thoxy]acetyl-2-[2-(2-aminoethoxy)ethoxy]acetyl

<400>  8

His Xaa Glu Gly Thr Phe Thr Ser Asp Val Ser Ser Tyr Leu Glu Gly 
1               5                   10                  15      


Gly Gln Ala Ala Lys Glu Phe Ile Ala Trp Leu Val Arg Gly Arg Gly 
            20                  25                  30          


<210>  9
<211>  32
<212>  PRT
<213>  artificial sequence

<220>
<223>  Peptide fragment


<220>
<221>  Misc_feature
<222>  (2)..(2)
<223>  Xaa is Aib

<220>
<221>  Misc_feature
<222>  (20)..(20)
<223>  wherein Lys is Lys- 
       N-(17-carboxy-1-oxoheptadecyl)-L-gamma-glutamyl-2-[2-(2-aminoethoxy)e
       thoxy]acetyl-2-[2-(2-aminoethoxy)ethoxy]acetyl

<400>  9

His Xaa Glu Gly Thr Phe Thr Ser Asp Val Ser Ser Tyr Leu Glu Gly 
1               5                   10                  15      


Gln Ala Ala Lys Glu Phe Ile Ala Trp Leu Val Arg Gly Gly Arg Gly 
            20                  25                  30          


<210>  10
<211>  32
<212>  PRT
<213>  artificial sequence

<220>
<223>  Peptide fragment


<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Xaa is Aib

<220>
<221>  misc_feature
<222>  (20)..(20)
<223>  wherein Lys is Lys- 
       N-(17-carboxy-1-oxoheptadecyl)-L-gamma-glutamyl-2-[2-(2-aminoethoxy)e
       thoxy]acetyl-2-[2-(2-aminoethoxy)ethoxy]acetyl

<400>  10

His Xaa Glu Gly Thr Phe Thr Ser Asp Val Ser Ser Tyr Leu Glu Gly 
1               5                   10                  15      


Gln Ala Ala Lys Glu Phe Ile Ala Trp Leu Val Arg Gly Arg Gly Gly 
            20                  25                  30          


<210>  11
<211>  32
<212>  PRT
<213>  artificial sequence

<220>
<223>  Peptide fragment


<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Xaa is Aib

<220>
<221>  misc_feature
<222>  (21)..(21)
<223>  wherein Lys is Lys- 
       N-(17-carboxy-1-oxoheptadecyl)-L-gamma-glutamyl-2-[2-(2-aminoethoxy)e
       thoxy]acetyl-2-[2-(2-aminoethoxy)ethoxy]acetyl

<400>  11

His Xaa Glu Gly Thr Phe Thr Ser Asp Val Ser Ser Tyr Leu Glu Gly 
1               5                   10                  15      


Gln Ala Ala Ala Lys Glu Phe Ile Ala Trp Leu Val Arg Gly Arg Gly 
            20                  25                  30          


<210>  12
<211>  31
<212>  PRT
<213>  artificial sequence

<220>
<223>  Peptide fragment


<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Xaa is Aib

<220>
<221>  misc_feature
<222>  (20)..(20)
<223>  wherein Lys is Lys- 
       N-(17-carboxy-1-oxoheptadecyl)-L-gamma-glutamyl-2-[2-(2-aminoethoxy)e
       thoxy]acetyl-2-[2-(2-aminoethoxy)ethoxy]acetyl

<400>  12

His Xaa Glu Gly Thr Phe Thr Ser Asp Val Ser Ser Tyr Leu Glu Gly 
1               5                   10                  15      


Gln Ala Ala Lys Glu Phe Ile Ala Trp Leu Val Arg Gly Arg Gly 
            20                  25                  30      


<210>  13
<211>  31
<212>  PRT
<213>  artificial sequence

<220>
<223>  Peptide fragment


<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Xaa is Aib

<220>
<221>  misc_feature
<222>  (20)..(20)
<223>  wherein Lys is Lys- 
       N-(17-carboxy-1-oxoheptadecyl)-L-gamma-glutamyl-2-[2-(2-aminoethoxy)e
       thoxy]acetyl-2-[2-(2-aminoethoxy)ethoxy]acetyl

<400>  13

His Xaa Glu Gly Thr Phe Thr Ser Asp Val Ser Ser Tyr Leu Glu Gly 
1               5                   10                  15      


Gln Ala Ala Lys Glu Phe Ile Ala Trp Leu Val Arg Gly Arg Gly 
            20                  25                  30      


<210>  14
<211>  31
<212>  PRT
<213>  artificial sequence

<220>
<223>  Peptide fragment


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa is Aib

<220>
<221>  MISC_FEATURE
<222>  (20)..(20)
<223>  wherein Lys is Lys- 
       N-(17-carboxy-1-oxoheptadecyl)-L-gamma-glutamyl-2-[2-(2-aminoethoxy)e
       thoxy]acetyl-2-[2-(2-aminoethoxy)ethoxy]acetyl

<400>  14

His Xaa Glu Gly Thr Phe Thr Ser Asp Val Ser Ser Tyr Leu Glu Gly 
1               5                   10                  15      


Gln Ala Ala Lys Glu Phe Ile Ala Trp Leu Val Arg Gly Arg Gly 
            20                  25                  30      


<210>  15
<211>  31
<212>  PRT
<213>  artificial sequence

<220>
<223>  Peptide fragment


<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Xaa is Aib

<220>
<221>  misc_feature
<222>  (20)..(20)
<223>  wherein Lys is Lys- 
       N-(17-carboxy-1-oxoheptadecyl)-L-gamma-glutamyl-2-[2-(2-aminoethoxy)e
       thoxy]acetyl-2-[2-(2-aminoethoxy)ethoxy]acetyl

<400>  15

His Xaa Glu Gly Thr Phe Thr Ser Asp Val Ser Ser Tyr Leu Glu Gly 
1               5                   10                  15      


Gln Ala Ala Lys Glu Phe Ile Ala Trp Leu Val Arg Gly Arg Gly 
            20                  25                  30      


<210>  16
<211>  31
<212>  PRT
<213>  artificial sequence

<220>
<223>  Peptide fragment


<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Xaa is aib

<220>
<221>  misc_feature
<222>  (20)..(20)
<223>  wherein Lys is Lys- 
       N-(17-carboxy-1-oxoheptadecyl)-L-gamma-glutamyl-2-[2-(2-aminoethoxy)e
       thoxy]acetyl-2-[2-(2-aminoethoxy)ethoxy]acetyl

<400>  16

His Xaa Glu Gly Thr Phe Thr Ser Asp Val Ser Ser Tyr Leu Glu Gly 
1               5                   10                  15      


Gln Ala Ala Lys Glu Phe Ile Ala Trp Leu Val Arg Gly Arg Gly 
            20                  25                  30      


<210>  17
<211>  31
<212>  PRT
<213>  artificial sequence

<220>
<223>  Peptide fragment


<220>
<221>  misc_Feature
<222>  (2)..(2)
<223>  Xaa is Aib

<220>
<221>  misc_Feature
<222>  (20)..(20)
<223>  wherein Lys is Lys- 
       N-(17-carboxy-1-oxoheptadecyl)-L-gamma-glutamyl-2-[2-(2-aminoethoxy)e
       thoxy]acetyl-2-[2-(2-aminoethoxy)ethoxy]acetyl

<400>  17

His Xaa Glu Gly Thr Phe Thr Ser Asp Val Ser Ser Tyr Leu Glu Gly 
1               5                   10                  15      


Gln Ala Ala Lys Glu Phe Ile Ala Trp Leu Val Arg Gly Arg Gly 
            20                  25                  30      


<210>  18
<211>  31
<212>  PRT
<213>  artificial sequence

<220>
<223>  Peptide fragment


<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Xaa is Aib

<220>
<221>  misc_feature
<222>  (20)..(20)
<223>  wherein Lys is Lys- 
       N-(17-carboxy-1-oxoheptadecyl)-L-gamma-glutamyl-2-[2-(2-aminoethoxy)e
       thoxy]acetyl-2-[2-(2-aminoethoxy)ethoxy]acetyl

<400>  18

His Xaa Glu Gly Thr Phe Thr Ser Asp Val Ser Ser Tyr Leu Glu Gly 
1               5                   10                  15      


Gln Ala Ala Lys Glu Phe Ile Ala Trp Leu Val Arg Gly Arg Gly 
            20                  25                  30      


<210>  19
<211>  32
<212>  PRT
<213>  artificial sequence

<220>
<223>  Peptide fragment


<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  xaa is aib

<220>
<221>  misc_feature
<222>  (20)..(20)
<223>  wherein Lys is Lys- 
       N-(17-carboxy-1-oxoheptadecyl)-L-gamma-glutamyl-2-[2-(2-aminoethoxy)e
       thoxy]acetyl-2-[2-(2-aminoethoxy)ethoxy]acetyl

<400>  19

His Xaa Glu Gly Thr Phe Thr Ser Asp Val Ser Ser Tyr Leu Glu Gly 
1               5                   10                  15      


Glu Ala Ala Lys Glu Phe Ile Ala Trp Leu Val Arg Gly Arg Gly Gly 
            20                  25                  30          


<210>  20
<211>  32
<212>  PRT
<213>  artificial sequence

<220>
<223>  Peptide fragment


<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Xaa is Aib

<220>
<221>  misc_feature
<222>  (8)..(8)
<223>  Asp is B-Asp

<220>
<221>  misc_feature
<222>  (20)..(20)
<223>  wherein Lys is Lys- 
       N-(17-carboxy-1-oxoheptadecyl)-L-gamma-glutamyl-2-[2-(2-aminoethoxy)e
       thoxy]acetyl-2-[2-(2-aminoethoxy)ethoxy]acetyl

<400>  20

His Xaa Glu Gly Thr Phe Thr Ser Asp Val Ser Ser Tyr Leu Glu Gly 
1               5                   10                  15      


Glu Ala Ala Lys Glu Phe Ile Ala Trp Leu Val Arg Gly Arg Gly Gly 
            20                  25                  30          


<210>  21
<211>  31
<212>  PRT
<213>  artificial sequence

<220>
<223>  Peptide fragment


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Xaa is Aib


<220>
<221>  misc_feature
<222>  (19)..(19)
<223>  wherein Lys is Lys- 
       N-(17-carboxy-1-oxoheptadecyl)-L-gamma-glutamyl-2-[2-(2-aminoethoxy)e
       thoxy]acetyl-2-[2-(2-aminoethoxy)ethoxy]acetyl

<400>  21

Xaa Glu Gly Thr Phe Thr Ser Asp Val Ser Ser Tyr Leu Glu Gly Gln 
1               5                   10                  15      


Ala Ala Lys Glu Phe Ile Ala Trp Leu Val Arg Gly Arg Gly Gly 
            20                  25                  30      


<210>  22
<211>  30
<212>  PRT
<213>  artificial sequence

<220>
<223>  Peptide fragment


<220>
<221>  misc_feature
<222>  (18)..(18)
<223>  wherein Lys is Lys- 
       N-(17-carboxy-1-oxoheptadecyl)-L-gamma-glutamyl-2-[2-(2-aminoethoxy)e
       thoxy]acetyl-2-[2-(2-aminoethoxy)ethoxy]acetyl

<400>  22

Glu Gly Thr Phe Thr Ser Asp Val Ser Ser Tyr Leu Glu Gly Gln Ala 
1               5                   10                  15      


Ala Lys Glu Phe Ile Ala Trp Leu Val Arg Gly Arg Gly Gly 
            20                  25                  30  


<210>  23
<211>  16
<212>  PRT
<213>  artificial sequence

<220>
<223>  Peptide fragment


<220>
<221>  misc_Feature
<222>  (1)..(1)
<223>  Wherein His is His(BOC)

<220>
<221>  misc_Feature
<222>  (2)..(2)
<223>  Xaa is Aib

<220>
<221>  misc_Feature
<222>  (3)..(3)
<223>  Glu has side chain protecting group

<220>
<221>  misc_Feature
<222>  (5)..(5)
<223>  Thr has side chain protecting group

<220>
<221>  misc_Feature
<222>  (7)..(7)
<223>  Thr has side chain protecting group

<220>
<221>  misc_Feature
<222>  (8)..(8)
<223>  Ser has side chain protecting group

<220>
<221>  misc_Feature
<222>  (9)..(9)
<223>  Asp has side chain protecting group

<220>
<221>  misc_Feature
<222>  (11)..(11)
<223>  Ser has side chain protecting group

<220>
<221>  misc_Feature
<222>  (13)..(13)
<223>  Tyr has side chain protecting group

<220>
<221>  misc_Feature
<222>  (15)..(15)
<223>  Glu has side chain protecting group

<220>
<221>  misc_Feature
<222>  (16)..(16)
<223>  Gly is Gly-O

<400>  23

His Xaa Glu Gly Thr Phe Thr Ser Asp Val Ser Ser Tyr Leu Glu Gly 
1               5                   10                  15      


<210>  24
<211>  16
<212>  PRT
<213>  artificial sequence

<220>
<223>  Peptide fragment


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  His is Boc-His(Boc)

<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Xaa is Aib

<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  Glu is Glu(OtBu)

<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  Thr is Thr(TBu)

<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  Thr is Thr(TBu)

<220>
<221>  misc_feature
<222>  (8)..(8)
<223>  Ser is Ser(TBu)

<220>
<221>  misc_feature
<222>  (9)..(9)
<223>  Asp is Asp(OtBu)

<220>
<221>  misc_feature
<222>  (11)..(11)
<223>  Ser is Ser(tBU)

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  Ser is Ser(psi Me, Me, Pro)

<220>
<221>  misc_feature
<222>  (13)..(13)
<223>  Tyr is Tyr (tBu)

<220>
<221>  misc_feature
<222>  (15)..(15)
<223>  Glu is Glu(OtBu)

<220>
<221>  misc_feature
<222>  (16)..(16)
<223>  Gly is Gly-OH

<400>  24

His Xaa Glu Gly Thr Phe Thr Ser Asp Val Ser Ser Tyr Leu Glu Gly 
1               5                   10                  15      


<210>  25
<211>  16
<212>  PRT
<213>  artificial sequence

<220>
<223>  Peptide fragment


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  His is Fmoc-His(Boc)-

<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Xaa is Aib

<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  Glu is Glu(OtBu)

<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  Thr is Thr(TBu)

<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  Thr is Thr(TBu)

<220>
<221>  misc_feature
<222>  (8)..(8)
<223>  Ser is Ser(TBu)

<220>
<221>  misc_feature
<222>  (9)..(9)
<223>  Asp is Asp(OtBu)

<220>
<221>  misc_feature
<222>  (11)..(11)
<223>  Ser is Ser(tBU)

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  Ser is Ser(psi Me, Me, Pro)

<220>
<221>  misc_feature
<222>  (13)..(13)
<223>  Tyr is Tyr (tBu)

<220>
<221>  misc_feature
<222>  (15)..(15)
<223>  Glu is Glu(OtBu)

<220>
<221>  misc_feature
<222>  (16)..(16)
<223>  Gly is Gly-OH

<400>  25

His Xaa Glu Gly Thr Phe Thr Ser Asp Val Ser Ser Tyr Leu Glu Gly 
1               5                   10                  15      


<210>  26
<211>  16
<212>  PRT
<213>  artificial sequence

<220>
<223>  Peptide fragment


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  His is Cbz-His(Boc)-

<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Xaa is Aib

<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  Glu is Glu(OtBu)

<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  Thr is Thr(TBu)

<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  Thr is Thr(TBu)

<220>
<221>  misc_feature
<222>  (8)..(8)
<223>  Ser is Ser(TBu)

<220>
<221>  misc_feature
<222>  (9)..(9)
<223>  Asp is Asp(OtBu)

<220>
<221>  misc_feature
<222>  (11)..(11)
<223>  Ser is Ser(tBU)

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  Ser is Ser(psi Me, Me, Pro)

<220>
<221>  misc_feature
<222>  (13)..(13)
<223>  Tyr is Tyr (tBu)

<220>
<221>  misc_feature
<222>  (15)..(15)
<223>  Glu is Glu(OtBu)

<220>
<221>  misc_feature
<222>  (16)..(16)
<223>  Gly is Gly-OH

<400>  26

His Xaa Glu Gly Thr Phe Thr Ser Asp Val Ser Ser Tyr Leu Glu Gly 
1               5                   10                  15      


<210>  27
<211>  16
<212>  PRT
<213>  artificial sequence

<220>
<223>  Peptide fragment


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  His is Boc-His(Boc)

<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Xaa is Aib

<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  Glu is Glu(OtBu)

<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  Thr is Thr(TBu)

<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  Thr is Thr(TBu)

<220>
<221>  misc_feature
<222>  (8)..(8)
<223>  Ser is Ser(TBu)

<220>
<221>  misc_feature
<222>  (9)..(9)
<223>  Asp is Asp(OtBu)

<220>
<221>  misc_feature
<222>  (11)..(11)
<223>  Ser is Ser(tBU)

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  Ser is Ser(psi Me, Me, Pro)

<220>
<221>  misc_feature
<222>  (13)..(13)
<223>  Tyr is Tyr (tBu)

<220>
<221>  misc_feature
<222>  (15)..(15)
<223>  Glu is Glu(OtBu)

<220>
<221>  misc_feature
<222>  (16)..(16)
<223>  Gly is Gly-oSU

<400>  27

His Xaa Glu Gly Thr Phe Thr Ser Asp Val Ser Ser Tyr Leu Glu Gly 
1               5                   10                  15      


<210>  28
<211>  16
<212>  PRT
<213>  artificial sequence

<220>
<223>  Peptide fragment


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  His is Fmoc-His(Boc)

<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Xaa is Aib

<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  Glu is Glu(OtBu)

<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  Thr is Thr(TBu)

<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  Thr is Thr(TBu)

<220>
<221>  misc_feature
<222>  (8)..(8)
<223>  Ser is Ser(TBu)

<220>
<221>  misc_feature
<222>  (9)..(9)
<223>  Asp is Asp(OtBu)

<220>
<221>  misc_feature
<222>  (11)..(11)
<223>  Ser is Ser(tBU)

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  Ser is Ser(psi Me, Me, Pro)

<220>
<221>  misc_feature
<222>  (13)..(13)
<223>  Tyr is Tyr (tBu)

<220>
<221>  misc_feature
<222>  (15)..(15)
<223>  Glu is Glu(OtBu)

<220>
<221>  misc_feature
<222>  (16)..(16)
<223>  Gly is Gly-oSU

<400>  28

His Xaa Glu Gly Thr Phe Thr Ser Asp Val Ser Ser Tyr Leu Glu Gly 
1               5                   10                  15      


<210>  29
<211>  16
<212>  PRT
<213>  artificial sequence

<220>
<223>  Peptide fragment


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  His is Cbz-His(Boc)

<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Xaa is Aib

<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  Glu is Glu(OtBu)

<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  Thr is Thr(TBu)

<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  Thr is Thr(TBu)

<220>
<221>  misc_feature
<222>  (8)..(8)
<223>  Ser is Ser(TBu)

<220>
<221>  misc_feature
<222>  (9)..(9)
<223>  Asp is Asp(OtBu)

<220>
<221>  misc_feature
<222>  (11)..(11)
<223>  Ser is Ser(tBU)

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  Ser is Ser(psi Me, Me, Pro)

<220>
<221>  misc_feature
<222>  (13)..(13)
<223>  Tyr is Tyr (tBu)

<220>
<221>  misc_feature
<222>  (15)..(15)
<223>  Glu is Glu(OtBu)

<220>
<221>  misc_feature
<222>  (16)..(16)
<223>  Gly is Gly-oSU

<400>  29

His Xaa Glu Gly Thr Phe Thr Ser Asp Val Ser Ser Tyr Leu Glu Gly 
1               5                   10                  15      


<210>  30
<211>  16
<212>  PRT
<213>  artificial sequence

<220>
<223>  Peptide fragment


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  His is Boc-His(Boc)

<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Xaa is Aib

<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  Glu is Glu(OtBu)

<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  Thr is Thr(TBu)

<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  Thr is Thr(TBu)

<220>
<221>  misc_feature
<222>  (8)..(8)
<223>  Ser is Ser(TBu)

<220>
<221>  misc_feature
<222>  (9)..(9)
<223>  Asp is Asp(OtBu)

<220>
<221>  misc_feature
<222>  (11)..(11)
<223>  Ser is Ser(tBU)

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  Ser is Ser(psi Me, Me, Pro)

<220>
<221>  misc_feature
<222>  (13)..(13)
<223>  Tyr is Tyr (tBu)

<220>
<221>  misc_feature
<222>  (15)..(15)
<223>  Glu is Glu(OtBu)

<220>
<221>  misc_feature
<222>  (16)..(16)
<223>  Gly is Gly-oBt

<400>  30

His Xaa Glu Gly Thr Phe Thr Ser Asp Val Ser Ser Tyr Leu Glu Gly 
1               5                   10                  15      


<210>  31
<211>  16
<212>  PRT
<213>  artificial sequence

<220>
<223>  Peptide fragment


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  His is Fmoc-His(Boc)

<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Xaa is Aib

<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  Glu is Glu(OtBu)

<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  Thr is Thr(TBu)

<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  Thr is Thr(TBu)

<220>
<221>  misc_feature
<222>  (8)..(8)
<223>  Ser is Ser(TBu)

<220>
<221>  misc_feature
<222>  (9)..(9)
<223>  Asp is Asp(OtBu)

<220>
<221>  misc_feature
<222>  (11)..(11)
<223>  Ser is Ser(tBU)

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  Ser is Ser(psi Me, Me, Pro)

<220>
<221>  misc_feature
<222>  (13)..(13)
<223>  Tyr is Tyr (tBu)

<220>
<221>  misc_feature
<222>  (15)..(15)
<223>  Glu is Glu(OtBu)

<220>
<221>  misc_feature
<222>  (16)..(16)
<223>  Gly is Gly-oBt

<400>  31

His Xaa Glu Gly Thr Phe Thr Ser Asp Val Ser Ser Tyr Leu Glu Gly 
1               5                   10                  15      


<210>  32
<211>  16
<212>  PRT
<213>  artificial sequence

<220>
<223>  Peptide fragment


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  His is Cbz-His(Boc)

<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Xaa is Aib

<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  Glu is Glu(OtBu)

<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  Thr is Thr(TBu)

<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  Thr is Thr(TBu)

<220>
<221>  misc_feature
<222>  (8)..(8)
<223>  Ser is Ser(TBu)

<220>
<221>  misc_feature
<222>  (9)..(9)
<223>  Asp is Asp(OtBu)

<220>
<221>  misc_feature
<222>  (11)..(11)
<223>  Ser is Ser(tBU)

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  Ser is Ser(psi Me, Me, Pro)

<220>
<221>  misc_feature
<222>  (13)..(13)
<223>  Tyr is Tyr (tBu)

<220>
<221>  misc_feature
<222>  (15)..(15)
<223>  Glu is Glu(OtBu)

<220>
<221>  misc_feature
<222>  (16)..(16)
<223>  Gly is Gly-oBt

<400>  32

His Xaa Glu Gly Thr Phe Thr Ser Asp Val Ser Ser Tyr Leu Glu Gly 
1               5                   10                  15      


<210>  33
<211>  16
<212>  PRT
<213>  artificial sequence

<220>
<223>  Peptide fragment


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  His is Boc-His(Boc)

<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Xaa is Aib

<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  Glu is Glu(OtBu)

<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  Thr is Thr(TBu)

<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  Thr is Thr(TBu)

<220>
<221>  misc_feature
<222>  (8)..(8)
<223>  Ser is Ser(TBu)

<220>
<221>  misc_feature
<222>  (9)..(9)
<223>  Asp is Asp(OtBu)

<220>
<221>  misc_feature
<222>  (11)..(11)
<223>  Ser is Ser(tBU)

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  Ser is Ser(psi Me, Me, Pro)

<220>
<221>  misc_feature
<222>  (13)..(13)
<223>  Tyr is Tyr (tBu)

<220>
<221>  misc_feature
<222>  (15)..(15)
<223>  Glu is Glu(OtBu)

<220>
<221>  misc_feature
<222>  (16)..(16)
<223>  Gly is Gly-oPfp

<400>  33

His Xaa Glu Gly Thr Phe Thr Ser Asp Val Ser Ser Tyr Leu Glu Gly 
1               5                   10                  15      


<210>  34
<211>  16
<212>  PRT
<213>  artificial sequence

<220>
<223>  Peptide fragment


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  His is Fmoc-His(Boc)

<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Xaa is Aib

<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  Glu is Glu(OtBu)

<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  Thr is Thr(TBu)

<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  Thr is Thr(TBu)

<220>
<221>  misc_feature
<222>  (8)..(8)
<223>  Ser is Ser(TBu)

<220>
<221>  misc_feature
<222>  (9)..(9)
<223>  Asp is Asp(OtBu)

<220>
<221>  misc_feature
<222>  (11)..(11)
<223>  Ser is Ser(tBU)

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  Ser is Ser(psi Me, Me, Pro)

<220>
<221>  misc_feature
<222>  (13)..(13)
<223>  Tyr is Tyr (tBu)

<220>
<221>  misc_feature
<222>  (15)..(15)
<223>  Glu is Glu(OtBu)

<220>
<221>  misc_feature
<222>  (16)..(16)
<223>  Gly is Gly-oPfp

<400>  34

His Xaa Glu Gly Thr Phe Thr Ser Asp Val Ser Ser Tyr Leu Glu Gly 
1               5                   10                  15      


<210>  35
<211>  16
<212>  PRT
<213>  artificial sequence

<220>
<223>  Peptide fragment


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  His is Cbz-His(Boc)

<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Xaa is Aib

<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  Glu is Glu(OtBu)

<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  Thr is Thr(TBu)

<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  Thr is Thr(TBu)

<220>
<221>  misc_feature
<222>  (8)..(8)
<223>  Ser is Ser(TBu)

<220>
<221>  misc_feature
<222>  (9)..(9)
<223>  Asp is Asp(OtBu)

<220>
<221>  misc_feature
<222>  (11)..(11)
<223>  Ser is Ser(tBU)

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  Ser is Ser(psi Me, Me, Pro)

<220>
<221>  misc_feature
<222>  (13)..(13)
<223>  Tyr is Tyr (tBu)

<220>
<221>  misc_feature
<222>  (15)..(15)
<223>  Glu is Glu(OtBu)

<220>
<221>  misc_feature
<222>  (16)..(16)
<223>  Gly is Gly-oPfp

<400>  35

His Xaa Glu Gly Thr Phe Thr Ser Asp Val Ser Ser Tyr Leu Glu Gly 
1               5                   10                  15      


<210>  36
<211>  15
<212>  PRT
<213>  artificial sequence

<220>
<223>  Peptide fragment


<220>
<221>  misc_Feature
<222>  (1)..(1)
<223>  Xaa is Aib

<220>
<221>  misc_Feature
<222>  (2)..(2)
<223>  Glu has side chain protecting group

<220>
<221>  misc_Feature
<222>  (4)..(4)
<223>  Thr has side chain protecting group

<220>
<221>  misc_Feature
<222>  (6)..(6)
<223>  Thr has side chain protecting group

<220>
<221>  misc_Feature
<222>  (7)..(7)
<223>  Ser has side chain protecting group

<220>
<221>  misc_Feature
<222>  (8)..(8)
<223>  Asp has side chain protecting group

<220>
<221>  misc_Feature
<222>  (10)..(11)
<223>  Ser has side chain protecting group

<220>
<221>  misc_Feature
<222>  (12)..(12)
<223>  Tyr has side chain protecting group

<220>
<221>  misc_Feature
<222>  (14)..(14)
<223>  Glu has side chain protecting group

<220>
<221>  misc_Feature
<222>  (16)..(16)
<223>  Gly is Gly-O

<400>  36

Xaa Glu Gly Thr Phe Thr Ser Asp Val Ser Ser Tyr Leu Glu Gly 
1               5                   10                  15  


<210>  37
<211>  15
<212>  PRT
<213>  artificial sequence

<220>
<223>  Peptide fragment


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Xaa is Fmoc-Aib

<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Glu is Glu(OtBu)

<220>
<221>  misc_feature
<222>  (4)..(4)
<223>  Thr is Thr(tBu)

<220>
<221>  misc_feature
<222>  (6)..(6)
<223>  Thr is Thr(tBu)

<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  Ser is Ser(tBu)

<220>
<221>  misc_feature
<222>  (8)..(8)
<223>  Asp is Asp(OtBu)

<220>
<221>  misc_feature
<222>  (10)..(10)
<223>  Ser is Ser(tBu)

<220>
<221>  misc_feature
<222>  (11)..(11)
<223>  Ser is Ser( Psi, me, me Pro)

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  Tyr is Tyr(tBu)

<220>
<221>  misc_feature
<222>  (14)..(14)
<223>  Glu is Glu(OtBu)

<220>
<221>  misc_feature
<222>  (15)..(15)
<223>  Gly is Gly-OH

<400>  37

Xaa Glu Gly Thr Phe Thr Ser Asp Val Ser Ser Tyr Leu Glu Gly 
1               5                   10                  15  


<210>  38
<211>  15
<212>  PRT
<213>  artificial sequence

<220>
<223>  Peptide fragment


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Xaa is Cbz-Aib

<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Glu is Glu(OtBu)

<220>
<221>  misc_feature
<222>  (4)..(4)
<223>  Thr is Thr(tBu)

<220>
<221>  misc_feature
<222>  (6)..(6)
<223>  Thr is Thr(tBu)

<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  Ser is Ser(tBu)

<220>
<221>  misc_feature
<222>  (8)..(8)
<223>  Asp is Asp(OtBu)

<220>
<221>  misc_feature
<222>  (10)..(10)
<223>  Ser is Ser(tBu)

<220>
<221>  misc_feature
<222>  (11)..(11)
<223>  Ser is Ser( Psi, me, me Pro)

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  Tyr is Tyr(tBu)

<220>
<221>  misc_feature
<222>  (14)..(14)
<223>  Glu is Glu(OtBu)

<220>
<221>  misc_feature
<222>  (15)..(15)
<223>  Gly is Gly-OH

<400>  38

Xaa Glu Gly Thr Phe Thr Ser Asp Val Ser Ser Tyr Leu Glu Gly 
1               5                   10                  15  


<210>  39
<211>  15
<212>  PRT
<213>  artificial sequence

<220>
<223>  Peptide fragment


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Xaa is Cbz-Aib

<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Glu is Glu(OtBu)

<220>
<221>  misc_feature
<222>  (4)..(4)
<223>  Thr is Thr(tBu)

<220>
<221>  misc_feature
<222>  (6)..(6)
<223>  Thr is Thr(tBu)

<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  Ser is Ser(tBu)

<220>
<221>  misc_feature
<222>  (8)..(8)
<223>  Asp is Asp(OtBu)

<220>
<221>  misc_feature
<222>  (10)..(10)
<223>  Ser is Ser(tBu)

<220>
<221>  misc_feature
<222>  (11)..(11)
<223>  Ser is Ser( Psi, me, me Pro)

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  Tyr is Tyr(tBu)

<220>
<221>  misc_feature
<222>  (14)..(14)
<223>  Glu is Glu(OtBu)

<220>
<221>  misc_feature
<222>  (15)..(15)
<223>  Gly is Gly-OSu

<400>  39

Xaa Glu Gly Thr Phe Thr Ser Asp Val Ser Ser Tyr Leu Glu Gly 
1               5                   10                  15  


<210>  40
<211>  15
<212>  PRT
<213>  artificial sequence

<220>
<223>  Peptide fragment


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Xaa is Fmoc-Aib

<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Glu is Glu(OtBu)

<220>
<221>  misc_feature
<222>  (4)..(4)
<223>  Thr is Thr(tBu)

<220>
<221>  misc_feature
<222>  (6)..(6)
<223>  Thr is Thr(tBu)

<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  Ser is Ser(tBu)

<220>
<221>  misc_feature
<222>  (8)..(8)
<223>  Asp is Asp(OtBu)

<220>
<221>  misc_feature
<222>  (10)..(10)
<223>  Ser is Ser(tBu)

<220>
<221>  misc_feature
<222>  (11)..(11)
<223>  Ser is Ser( Psi, me, me Pro)

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  Tyr is Tyr(tBu)

<220>
<221>  misc_feature
<222>  (14)..(14)
<223>  Glu is Glu(OtBu)

<220>
<221>  misc_feature
<222>  (15)..(15)
<223>  Gly is Gly-OBt

<400>  40

Xaa Glu Gly Thr Phe Thr Ser Asp Val Ser Ser Tyr Leu Glu Gly 
1               5                   10                  15  


<210>  41
<211>  15
<212>  PRT
<213>  artificial sequence

<220>
<223>  Peptide fragment


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Xaa is Cbz-Aib

<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Glu is Glu(OtBu)

<220>
<221>  misc_feature
<222>  (4)..(4)
<223>  Thr is Thr(tBu)

<220>
<221>  misc_feature
<222>  (6)..(6)
<223>  Thr is Thr(tBu)

<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  Ser is Ser(tBu)

<220>
<221>  misc_feature
<222>  (8)..(8)
<223>  Asp is Asp(OtBu)

<220>
<221>  misc_feature
<222>  (10)..(10)
<223>  Ser is Ser(tBu)

<220>
<221>  misc_feature
<222>  (11)..(11)
<223>  Ser is Ser( Psi, me, me Pro)

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  Tyr is Tyr(tBu)

<220>
<221>  misc_feature
<222>  (14)..(14)
<223>  Glu is Glu(OtBu)

<220>
<221>  misc_feature
<222>  (15)..(15)
<223>  Gly is Gly-OBt

<400>  41

Xaa Glu Gly Thr Phe Thr Ser Asp Val Ser Ser Tyr Leu Glu Gly 
1               5                   10                  15  


<210>  42
<211>  15
<212>  PRT
<213>  artificial sequence

<220>
<223>  Peptide fragment


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Xaa is Fmoc-Aib

<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Glu is Glu(OtBu)

<220>
<221>  misc_feature
<222>  (4)..(4)
<223>  Thr is Thr(tBu)

<220>
<221>  misc_feature
<222>  (6)..(6)
<223>  Thr is Thr(tBu)

<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  Ser is Ser(tBu)

<220>
<221>  misc_feature
<222>  (8)..(8)
<223>  Asp is Asp(OtBu)

<220>
<221>  misc_feature
<222>  (10)..(10)
<223>  Ser is Ser(tBu)

<220>
<221>  misc_feature
<222>  (11)..(11)
<223>  Ser is Ser( Psi, me, me Pro)

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  Tyr is Tyr(tBu)

<220>
<221>  misc_feature
<222>  (14)..(14)
<223>  Glu is Glu(OtBu)

<220>
<221>  misc_feature
<222>  (15)..(15)
<223>  Gly is Gly-OPfp

<400>  42

Xaa Glu Gly Thr Phe Thr Ser Asp Val Ser Ser Tyr Leu Glu Gly 
1               5                   10                  15  


<210>  43
<211>  15
<212>  PRT
<213>  artificial sequence

<220>
<223>  Peptide fragment


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Xaa is Cbz-Aib

<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Glu is Glu(OtBu)

<220>
<221>  misc_feature
<222>  (4)..(4)
<223>  Thr is Thr(tBu)

<220>
<221>  misc_feature
<222>  (6)..(6)
<223>  Thr is Thr(tBu)

<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  Ser is Ser(tBu)

<220>
<221>  misc_feature
<222>  (8)..(8)
<223>  Asp is Asp(OtBu)

<220>
<221>  misc_feature
<222>  (10)..(10)
<223>  Ser is Ser(tBu)

<220>
<221>  misc_feature
<222>  (11)..(11)
<223>  Ser is Ser( Psi, me, me Pro)

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  Tyr is Tyr(tBu)

<220>
<221>  misc_feature
<222>  (14)..(14)
<223>  Glu is Glu(OtBu)

<220>
<221>  misc_feature
<222>  (15)..(15)
<223>  Gly is Gly-OPfp

<400>  43

Xaa Glu Gly Thr Phe Thr Ser Asp Val Ser Ser Tyr Leu Glu Gly 
1               5                   10                  15  


<210>  44
<211>  15
<212>  PRT
<213>  artificial sequence

<220>
<223>  Peptide fragment


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Xaa is Fmoc-Aib

<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Glu is Glu(OtBu)

<220>
<221>  misc_feature
<222>  (4)..(4)
<223>  Thr is Thr(tBu)

<220>
<221>  misc_feature
<222>  (6)..(6)
<223>  Thr is Thr(tBu)

<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  Ser is Ser(tBu)

<220>
<221>  misc_feature
<222>  (8)..(8)
<223>  Asp is Asp(OtBu)

<220>
<221>  misc_feature
<222>  (10)..(10)
<223>  Ser is Ser(tBu)

<220>
<221>  misc_feature
<222>  (11)..(11)
<223>  Ser is Ser( Psi, me, me Pro)

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  Tyr is Tyr(tBu)

<220>
<221>  misc_feature
<222>  (14)..(14)
<223>  Glu is Glu(OtBu)

<220>
<221>  misc_feature
<222>  (15)..(15)
<223>  Gly is Gly-Su

<400>  44

Xaa Glu Gly Thr Phe Thr Ser Asp Val Ser Ser Tyr Leu Glu Gly 
1               5                   10                  15  


<210>  45
<211>  15
<212>  PRT
<213>  artificial sequence

<220>
<223>  Peptide fragment


<220>
<221>  misc_Feature
<222>  (1)..(1)
<223>  Gln  has a side chain protecting group

<220>
<221>  misc_Feature
<222>  (4)..(4)
<223>  wherein Lys is Lys- 
       N-(17-carboxy-1-oxoheptadecyl)-L-gamma-glutamyl-2-[2-(2-aminoethoxy)e
       thoxy]acetyl-2-[2-(2-aminoethoxy)ethoxy]acetyl

<220>
<221>  misc_Feature
<222>  (5)..(5)
<223>  Wherein Glu has a side chain protecting group

<220>
<221>  misc_Feature
<222>  (9)..(9)
<223>  Wherein Trp has a protecting Group

<220>
<221>  misc_Feature
<222>  (12)..(12)
<223>  Wherein Arg has a side chain protecting group

<220>
<221>  misc_Feature
<222>  (14)..(14)
<223>  Wherein Arg has a side chain protecting group

<220>
<221>  misc_Feature
<222>  (15)..(15)
<223>  Wherein Gly is Gly-O

<400>  45

Gln Ala Ala Lys Glu Phe Ile Ala Trp Leu Val Arg Gly Arg Gly 
1               5                   10                  15  


<210>  46
<211>  15
<212>  PRT
<213>  artificial sequence

<220>
<223>  Peptide fragment

<400>  46

Gln Ala Ala Lys Glu Phe Ile Ala Trp Leu Val Arg Gly Arg Gly 
1               5                   10                  15  


<210>  47
<211>  15
<212>  PRT
<213>  artificial sequence

<220>
<223>  Peptide fragment


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Gln is Gln(Trt)

<220>
<221>  misc_feature
<222>  (4)..(4)
<223>  Lys is Lys 
       N-(17-carboxy(P)-1-oxoheptadecyl)-L-gamma-glutamyl(P)-2-[2-(2-aminoet
       hoxy)ethoxy]acetyl-2-[2-(2-aminoethoxy)ethoxy]acetyl

<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  Glu(OtBu)

<220>
<221>  misc_feature
<222>  (9)..(9)
<223>  Trp is Trp(Boc)

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  Arg is Arg(Pbf)

<220>
<221>  misc_feature
<222>  (14)..(14)
<223>  Arg is Arg(Pbf)

<220>
<221>  misc_feature
<222>  (15)..(15)
<223>  Gly is Gly-CTC

<400>  47

Gln Ala Ala Lys Glu Phe Ile Ala Trp Leu Val Arg Gly Arg Gly 
1               5                   10                  15  


<210>  48
<211>  15
<212>  PRT
<213>  artificial sequence

<220>
<223>  Peptide fragment


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Gln is H-Gln with a side protecting group

<220>
<221>  misc_feature
<222>  (1)..(4)
<223>  Lys is Lys 
       N-(17-carboxy(P)-1-oxoheptadecyl)-L-gamma-glutamyl(P)-2-[2-(2-aminoet
       hoxy)ethoxy]acetyl-2-[2-(2-aminoethoxy)ethoxy]acetyl

<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  Glu is Glu with a side chain protecting group

<220>
<221>  misc_feature
<222>  (9)..(9)
<223>  Trp is a Trp with a side chain protecting group

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  Arg is  Arg with a side chain protecting group

<220>
<221>  misc_feature
<222>  (14)..(14)
<223>  Arg is  Arg with a side chain protecting group

<220>
<221>  misc_feature
<222>  (15)..(15)
<223>  Gly is Gly with H, or a solid support, e.g., a CTC or Wang resin 
       and wherein P4 is selected from H or a protecting group

<400>  48

Gln Ala Ala Lys Glu Phe Ile Ala Trp Leu Val Arg Gly Arg Gly 
1               5                   10                  15  


<210>  49
<211>  16
<212>  PRT
<213>  artificial sequence

<220>
<223>  Peptide fragment


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  His is Boc-His(Boc)

<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Xaa is Aib

<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  Glu is a Glu with a side chain protecting group

<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  Thr is a Thr with a Protecting group

<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  Thr is a Thr with a Protecting group

<220>
<221>  misc_feature
<222>  (8)..(8)
<223>  Ser is a Ser with a Protecting group

<220>
<221>  misc_feature
<222>  (9)..(9)
<223>  Asp is an Asp with a side chain protecting group

<220>
<221>  misc_feature
<222>  (11)..(11)
<223>  Ser is a Ser with a Side Chain Protecting Group

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  Ser is a Ser with a Side Chain Protecting Group

<220>
<221>  misc_feature
<222>  (13)..(13)
<223>  Tyr is a Tyr with a Side Chain Protecting Group

<220>
<221>  misc_feature
<222>  (15)..(15)
<223>  Glu is a Glu with a Side Chain Protecting Group

<220>
<221>  misc_feature
<222>  (16)..(16)
<223>  Gly is a Gly-O

<400>  49

His Xaa Glu Gly Thr Phe Thr Ser Asp Val Ser Ser Tyr Leu Glu Gly 
1               5                   10                  15      


<210>  50
<211>  16
<212>  PRT
<213>  artificial sequence

<220>
<223>  Peptide fragment


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  His is a P1-His(Boc)

<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Xaa is a Aib

<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  Glu is a Glu with a protecting group

<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  Thr is a Thr with a Protecting Group

<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  Thr is a Thr with a protecting group

<220>
<221>  misc_feature
<222>  (8)..(8)
<223>  Ser is a Ser with a Protecting Group

<220>
<221>  misc_feature
<222>  (9)..(9)
<223>  Asp is a Asp with a Protecting Group

<220>
<221>  misc_feature
<222>  (11)..(11)
<223>  Ser is a Ser with a Protecting Group

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  Ser is a Ser with a Protecting Group

<220>
<221>  misc_feature
<222>  (13)..(13)
<223>  Tyr is a Tyr with a Protecting Group

<220>
<221>  misc_feature
<222>  (14)..(14)
<223>  Leu is a Leu with a Protecting Group

<220>
<221>  misc_feature
<222>  (15)..(15)
<223>  Glu is a Glu with a Protecting Group

<220>
<221>  misc_feature
<222>  (16)..(16)
<223>  Gly is Gly-OH

<400>  50

His Xaa Glu Gly Thr Phe Thr Ser Asp Val Ser Ser Tyr Leu Glu Gly 
1               5                   10                  15      


<210>  51
<211>  16
<212>  PRT
<213>  artificial sequence

<220>
<223>  Peptide fragment


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  His is Boc-His(Boc)

<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Xaa is Aib

<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  Glu is Glu(OtBu)

<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  Thr is Thr(TBu)

<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  Thr is Thr(TBu)

<220>
<221>  misc_feature
<222>  (8)..(8)
<223>  Ser is Ser(TBu)

<220>
<221>  misc_feature
<222>  (9)..(9)
<223>  Asp is Asp(OtBu)

<220>
<221>  misc_feature
<222>  (11)..(11)
<223>  Ser is Ser(tBU)

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  Ser is Ser(psi Me, Me, Pro)

<220>
<221>  misc_feature
<222>  (13)..(13)
<223>  Tyr is Tyr (tBu)

<220>
<221>  misc_feature
<222>  (15)..(15)
<223>  Glu is Glu(OtBu)

<220>
<221>  misc_feature
<222>  (16)..(16)
<223>  Gly is Gly-OP2

<400>  51

His Xaa Glu Gly Thr Phe Thr Ser Asp Val Ser Ser Tyr Leu Glu Gly 
1               5                   10                  15      


