                         SEQUENCE LISTING

<110>  ELIGO BIOSCIENCE
 
<120>  TRANSCRIPTIONAL CONTROL IN PROKARYOTIC CELLS USING DNA-BINDING 
       REPRESSORS

<130>  B3216PC00

<160>  11    

<170>  PatentIn version 3.5

<210>  1
<211>  200
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PhlF repressor protein

<400>  1

Met Ala Arg Thr Pro Ser Arg Ser Ser Ile Gly Ser Leu Arg Ser Pro 
1               5                   10                  15      


His Thr His Lys Ala Ile Leu Thr Ser Thr Ile Glu Ile Leu Lys Glu 
            20                  25                  30          


Cys Gly Tyr Ser Gly Leu Ser Ile Glu Ser Val Ala Arg Arg Ala Gly 
        35                  40                  45              


Ala Ser Lys Pro Thr Ile Tyr Arg Trp Trp Thr Asn Lys Ala Ala Leu 
    50                  55                  60                  


Ile Ala Glu Val Tyr Glu Asn Glu Ser Glu Gln Val Arg Lys Phe Pro 
65                  70                  75                  80  


Asp Leu Gly Ser Phe Lys Ala Asp Leu Asp Phe Leu Leu Arg Asn Leu 
                85                  90                  95      


Trp Lys Val Trp Arg Glu Thr Ile Cys Gly Glu Ala Phe Arg Cys Val 
            100                 105                 110         


Ile Ala Glu Ala Gln Leu Asp Pro Ala Thr Leu Thr Gln Leu Lys Asp 
        115                 120                 125             


Gln Phe Met Glu Arg Arg Arg Glu Met Pro Lys Lys Leu Val Glu Asn 
    130                 135                 140                 


Ala Ile Ser Asn Gly Glu Leu Pro Lys Asp Thr Asn Arg Glu Leu Leu 
145                 150                 155                 160 


Leu Asp Met Ile Phe Gly Phe Cys Trp Tyr Arg Leu Leu Thr Glu Gln 
                165                 170                 175     


Leu Thr Val Glu Gln Asp Ile Glu Glu Phe Thr Phe Leu Leu Ile Asn 
            180                 185                 190         


Gly Val Cys Pro Gly Thr Gln Arg 
        195                 200 


<210>  2
<211>  600
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence encoding PhlF repressor protein

<400>  2
atggcacgta ccccgtcacg tagtagcatt ggtagcctgc gtagtccgca tacccataaa       60

gcaattctga ccagtaccat cgagatcctg aaagaatgtg gttatagcgg actgagcatt      120

gaaagcgttg cacgtcgtgc cggagcaagc aaaccgacca tttatcgttg gtggacgaat      180

aaagcagcac tgattgccga agtgtatgaa aatgaaagcg aacaggtgcg taaatttccg      240

gatctgggta gctttaaagc agatctggat tttttactgc gtaatttatg gaaagtttgg      300

cgtgaaacta tttgcggtga agcatttcgt tgtgttattg cagaagctca gctggatcct      360

gcaaccctga cccagttaaa ggatcaattt atggaacgtc gtcgtgagat gccgaaaaaa      420

ctggttgaaa atgccattag caatggtgaa ctgccgaaag ataccaatcg tgaacttctt      480

ctggatatga tttttggttt ttgttggtat cgcctgttaa ccgaacagct gaccgttgaa      540

caggatattg aagaatttac cttccttctg attaatggtg tttgtccggg tactcagcgt      600


<210>  3
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PhlF promoter-operator sequence

<400>  3
tctgattcgt taccaattga catgatacga aacgtaccgt atcgttaagg t                51


<210>  4
<211>  6504
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid containing Cas9 under the control of a PphlF promoter and
       a constitutive sgRNA guide targeting lacZ

<400>  4
ttttgccgtt acgcactact ttagtcagtt ccgcagtacc gtcagtagct gaacaggagg       60

gacagtgttg ataaagcgcc aactttttgc gaaaatgttg cacgtaagac aactttcacc      120

ataatgaaat aagatcacta ctattttttg agttatcgag atttcgcaag ctaaggaagc      180

taaaatggag aaaaaaatca ctggatatac tacagttgat ataagtcaat ggcatcgtaa      240

agaacatttt gaggcattcc agtcagttgc tcagtgcacc tataaccaaa ccgttcagct      300

ggatattacg gcctttttaa aaaccgtaaa gaaaaataag cacaagtttt atccggcctt      360

tattcacatt cttgcccgcc tgatgaatgc acatccggag tttcgtatgg caatgaaaga      420

cggtgagctg gtgatatggg atagtgttca cccttgttac accgttttcc atgagcaaac      480

tgaaacgttt tcatcgctct ggtcagaata ccacgacgat ttccggcagt ttctacacat      540

atatagtcaa gatgtggcgt gttacggtga aaacttagca tatttcccta aagggtttat      600

cgaaaatatg tttttcgtca gtgccaatcc gtgggtgagt ttcaccagtt ttgatttaaa      660

cgtagcaaat atggacaact tcttcgcccc cgttttcact atgggaaaat attatacgca      720

aggcgacaag gtgttaatgc cactggcgat ccaagttcat catgccgttt gtgatggctt      780

ccatgtcggc agaatgctta atgaattaca acaatactgc gatgagtggc agggcggggc      840

gtaatccggc aaggaaacac tgaaaaaagc ccgcacctga cagtgcgggc tttttttttc      900

gaccaaaggt atgcacatgc tatagacttc tggtgctacc cgactgacag ctagctcagt      960

cctaggtata atgctagccg tcgtgactgg gaaaacccgt tttagagcta gaaatagcaa     1020

gttaaaataa ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt     1080

tgagatcaaa catccactag tccctttacg cacgttgtga tatgtagatg ataatcatta     1140

tcactttacg ggtcctttcc ggtgatccga caggttacgg ggcggcgacc tcgcgggttt     1200

tcgctattta tgaaaatttt ccggtttaag gcgtttccgt tcttcttcgt cataacttaa     1260

tgtttttatt taaaataccc tctgaaaaga aaggaaacga caggtgctga aagcgaggct     1320

ttttggggcg ttcggctaaa cccaaaagta aaaacccgcc gaagcgggtt ttaacgtaaa     1380

acaggtgaaa ctgactttaa cgttattagt caccgcccaa ctgactcaga tcaatacgag     1440

tttcatacag gccagtgatg ctctggtgaa taagagttgc atctaagact tctttagtgg     1500

aggtgtaacg tttacgatcg atcgtggtgt cgaagtattt gaaggctgcc ggagcaccaa     1560

gattagtcag cgtgaacagg tgaatgatgt tttctgcctg ttcacggatc ggtttatcac     1620

ggtgtttatt gtaggcgctc aggactttat ccagattcgc gtccgccaga attacgcgtt     1680

tgctaaactc agaaatctgt tcaataatct cgtccagata gtgtttgtgc tgttccacga     1740

acagctgttt ctgctcatta tcttccggac tacctttcag tttctcgtag tgcgaggcca     1800

gatacaggaa gttcacgtac ttggacggaa gggccaattc gttccctttt tgcagttcac     1860

ccgcagacgc taacatacgt ttacgaccat tttccagttc gaacagggaa tacttcggca     1920

gtttgataat cagatctttc tttacttcct tgtacccctt cgcttcgagg aagtcaatcg     1980

ggtttttttc gaaagagcta cgttccataa tggtgatgcc taaaagttct ttcactgatt     2040

tcagtttttt tgatttaccc ttttcgactt tggcgactac cagaacgctg tatgccacgg     2100

ttggagaatc gaagccaccg tatttcttcg gatcccagtc tttcttacgc gcgatcagtt     2160

tgtcggagtt gcgtttcggc agaatggatt ccttgctgaa accgccagtc tgtacctcag     2220

tctttttaac gatgtttact tgaggcatgg acagaacttt gcggacagta gcgaaatcac     2280

gacctttgtc ccacacgatc tcacccgttt cgccgttggt ttcgatcaga gggcgtttac     2340

gaatttcgcc attcgctaag gtgatttcgg ttttgaagaa attcataatg tttgagtaga     2400

aaaagtattt agcggttgct ttgccaattt cctgttcaga tttggcgatc attttgcgca     2460

cgtcgtaaac tttataatcg ccgtacacaa attcgctttc cagtttcgga tattttttga     2520

tcagtgccgt gccaacaact gcattcagat atgcgtcgtg cgcgtggtga tagttgttaa     2580

tctcgcgcac tttatagaat tggaaatctt tacggaagtc ggaaaccagc ttagatttca     2640

gggtaataac cttaacttcg cggatcagtt tgtcattttc atcgtattta gtgttcatac     2700

gagagtccag aatctgagca acatgttttg taatctggcg agtttctacc agttgacgtt     2760

taataaaacc cgccttatcc agctcagaca aaccgccacg ttcagctttg gtcaggttat     2820

caaatttacg ttgagtaatc agtttcgcgt taagcagttg acgccaatag ttcttcattt     2880

tcttaactac ttcttccgat ggaacgttgt cactcttccc acggttttta tcgctacggg     2940

tgagtacttt gttatcaatg gaatcatctt tcagaaaaga ctgcggaaca atgtggtcaa     3000

cgtcgtaatc ggagaggcgg ttgatgtcca gctcctgatc tacatacatg tcacgaccat     3060

tttgcaggta gtacaggtac agtttttcat tttgcagttg ggtattttca acagggtgtt     3120

ctttcagaat ctgagagcca agttctttaa taccctcctc gatacgtttc atgcgttcgc     3180

gggagttttt ctgtcccttc tgggtcgttt ggttttcacg agccatctcg ataacaatgt     3240

tttccggttt gtgacgaccc ataactttca caagctcatc caccactttc acggtttgca     3300

ggataccttt tttgatcgcc ggactacctg ctaaattggc gatatgctcg tgcagagaat     3360

caccttgacc agaaacctga gctttctgga tgtcctcttt gaaggtcagg gaatcgtcgt     3420

gaatcagttg catgaagtta cggtttgcga agccgtcaga tttcaggaag tccagaatag     3480

ttttaccgga ctgtttgtca cggataccgt taatcagttt acggctcaga cggccccagc     3540

cagtataacg gcgacgtttc agttgtttca ttactttatc gtcaaacagg tgagcatacg     3600

tcttcaagcg ttcttcaatc atttcacgat cttcgaacag agtcagggtc aggacgatgt     3660

cctctaagat gtcctcattt tcttcgttgt ccaagaaatc tttgtcttta atgatcttta     3720

ataaatcgtg atacgtgcct aaacttgcat taaaacgatc ttcaactcca ctaatctcga     3780

cggagtcgaa gcattcgatt ttcttgaagt agtcctcttt cagctgttta acggttactt     3840

tacggttggt tttaaacagt aaatcaacaa ttgctttttt ctgttcaccg ctcaggaatg     3900

caggtttacg catgccctca gtcacatact ttactttagt cagctcgttg tacacggtga     3960

agtattcgta cagaagggag tgtttcggca gtaccttttc gttcggcagg tttttatcaa     4020

agttagtcat gcgctcgatg aaggattggg cgcttgcgcc tttgtcaacc acttcctcga     4080

agttccacgg ggtaatagtt tcttcggatt tgcgagtcat ccacgcgaaa cgggagttgc     4140

cacgcgccag cgggccgaca taatacggga tacgaaaggt caggattttt tcgatttttt     4200

cacgattatc tttcaggaac gggtaaaaat cctcttggcg acgcaggata gcgtgcagtt     4260

cacctaagtg gatctggtgc ggaatgctac cgttgtcgaa ggtacgctgt ttacgaagca     4320

gatcctcacg attcagttta acaagtaatt cttccgtgcc atccattttt tctaagattg     4380

gcttgatgaa tttgtagaac tcctcttggc ttgcaccccc gtcaatataa cccgcgtagc     4440

cgtttttgga ttggtcaaag aagatctcct tgtacttctc cggcagttgt tgccgtacca     4500

gcgctttcag aagagtcaga tcttggtgat gttcgtcata gcgtttaatc atggatgcag     4560

aaagcggtgc tttggtgatt tcggtattca cacgaagaat atcagacagg agaatagcgt     4620

ctgataagtt ctttgccgcc agaaacagat cagcgtactg atctccgatc tgggccagta     4680

agttatccaa gtcatcgtca tacgtatctt tgctaagttg aagttttgcg tcctctgcca     4740

gatcgaagtt gcttttgaag ttcggggtca gtcccaagct aagagcgatc agattgccga     4800

acagtccgtt tttcttctca cccggcagtt gcgcaattaa gttttccaga cgacgggatt     4860

tggacaggcg tgcggacaag atcgctttcg cgtcaacacc gctagcatta attgggtttt     4920

cttcaaacag ctggttatag gtttgcacca gctggatgaa cagtttgtct acatcgctgt     4980

tgtctggatt caggtcgcct tcgatcagga aatgaccacg gaacttaatc atatgcgcta     5040

aggccagata gatcagacgc agatcggcct tatccgtcga atcgaccagc tttttgcgca     5100

ggtgatagat agtcgggtat ttttcgtgat acgctacttc gtccacaatg ttgccgaaga     5160

ttggatgacg ttcgtgcttt ttatcttctt ccaccagaaa tgattcttcc agacggtgaa     5220

aaaagctgtc atctacttta gccatttcgt tagagaagat ttcttgcagg tagcagatgc     5280

ggtttttacg acgggtgtaa cggcgacgag cggtgcgttt cagacgtgtt gcttctgcgg     5340

tttcgccgga atcgaacaga agcgcaccga ttaagttctt tttgatacta tggcgatctg     5400

tattacccag taccttgaac tttttagacg gaactttata ttcatcagtg atcaccgccc     5460

atccgacgct atttgtgccg atgtctaagc ctatgctgta tttcttatcc atagaaactt     5520

tctccttttt aagctccagt aaccttaacg atacggtacg tttcgtatca tgtcaattgg     5580

taacgaatca gattcagtac gtcgcgtgct acatttgaag agataaattg cactgaaatc     5640

tagaaatatt ttatctgatt aataagatga tcttcttgag atcgttttgg tctgcgcgta     5700

atctcttgct ctgaaaacga aaaaaccgcc ttgcagggcg gtttttcgaa ggttctctga     5760

gctaccaact ctttgaaccg aggtaactgg cttggaggag cgcagtcgcc aaaacttgtc     5820

ctttcagttt agccttatcc ggcgcatgac ttcaagacta actcctctaa atcaattacc     5880

agtggctgct gccagtggtg cttttgcatg tctttccggg ttggactcaa gacgatagtt     5940

accggataag gcgcagcggt cggactgaac ggggggttcg tgcatacagt ccagcttgga     6000

gcgaactgcc tacccggaac tgagtgtcag gcgtggaatg agacaaacgc ggccataaca     6060

gcggaatgac accggtaaac cgaaaggcag gaacaggaga gcgcacgagg gagccgccag     6120

ggggaaacgc ctggtatctt tatagtcctg tcaggtttcg ccaccactga tttgagcgtc     6180

agatttcgtg atgcttgtca ggggggcgga gcctatggaa aaacggcttt gccgcggccc     6240

tctcacttcc ctgttaagta tcttcctggc atcttccagg aaatctccgc cccgttcgta     6300

agccatttcc gctcgccgca gtcgaacgac cgagcgtagc gagtcagtga gcgaggaagc     6360

ggaatatatc ctgtatcaca tattctgctg acgcaccggt gcagcctttt ttctcctgcc     6420

acatgaagca cttcacttac accctcatca gtgccaacat agtaagccag tatacactcc     6480

gctagcgctg atgtccggcg gtgc                                            6504


<210>  5
<211>  4024
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  plasmid encoding the PhlF repressor protein

<400>  5
agcgatcgcg tatttcgtct cgctcaggcg caatcacgaa tgaataacgg tttggttgat       60

gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg      120

cataagcttt tgccattctc accggattca gtcgtcactc atggtgattt ctcacttgat      180

aaccttattt ttgacgaggg gaaattaata ggttgtattg atgttggacg agtcggaatc      240

gcagaccgat accaggatct tgccatccta tggaactgcc tcggtgagtt ttctccttca      300

ttacagaaac ggctttttca aaaatatggt attgataatc ctgatatgaa taaattgcag      360

tttcatttga tgctcgatga gtttttctaa atcagaattg gttaattggt tgtggaaacg      420

taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa      480

tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt      540

gagttttcgt tccactgagc gtcagacccc gttgatgata ccgctgcctt actgggtgca      600

ttagccagtc tgaatgacct gtcacgggat aatccgaagt ggtcagactg gaaaatcaga      660

gggcaggaac tgctgaacag caaaaagtca gatagcacca catagcagac ccgccataaa      720

acgccctgag aagcccgtga cgggcttttc ttgtattatg ggtagtttcc ttgcatgaat      780

ccataaaagg cgcctgtagt gccatttacc cccattcact gccagagccg tgagctgacc      840

aaaacgaaaa aagacgctcg aaagcgtctg ttttctggaa tttggtagcg attatggcct      900

agttaacgct gagtacccgg acaaacacca ttaatcagaa ggaaggtaaa ttcttcaata      960

tcctgttcaa cggtcagctg ttcggttaac aggcgatacc aacaaaaacc aaaaatcata     1020

tccagaagaa gttcacgatt ggtatctttc ggcagttcac cattgctaat ggcattttca     1080

accagttttt tcggcatctc acgacgacgt tccataaatt gatcctttaa ctgggtcagg     1140

gttgcaggat ccagctgagc ttctgcaata acacaacgaa atgcttcacc gcaaatagtt     1200

tcacgccaaa ctttccataa attacgcagt aaaaaatcca gatctgcttt aaagctaccc     1260

agatccggaa atttacgcac ctgttcgctt tcattttcat acacttcggc aatcagtgct     1320

gctttattcg tccaccaacg ataaatggtc ggtttgcttg ctccggcacg acgtgcaacg     1380

ctttcaatgc tcagtccgct ataaccacat tctttcagga tctcgatggt actggtcaga     1440

attgctttat gggtatgcgg actacgcagg ctaccaatgc tactacgtga cggggtacgt     1500

gccatagtac ctttctcctc tttaatgatt cgctagcatt gtacctagga ctgagctagc     1560

cgtaaaatcg tgctacattt gaagagataa attgcactga aatctagaaa tattttatct     1620

gattaataag atgatcttct tgagatcgtt ttggtctgcg gtcatctgcg tgaccgccta     1680

cggcggctgc ggcgccctac gggcttgctc tccgggcttc gccctgcgcg gtcgctgcgc     1740

tcccttgcca gcccgtggat atgtggacga tggccgcgag cggccaccgg ctggctcgct     1800

tcgctcggcc cgtggacaac cctgctggac aagctgatgg acaggctgcg cctgcccacg     1860

agcttgacca cagggattgc ccaccggcta cccagccttc gaccacatac ccaccggctc     1920

caactgcgcg gcctgcggcc ttgccccatc aattttttta attttctctg gggaaaagcc     1980

tccggcctgc ggcctgcgcg cttcgcttgc cggttggaca ccaagtggaa ggcgggtcaa     2040

ggctcgcgca gcgaccgcgc agcggcttgg ccttgacgcg cctggaacga cccaagccta     2100

tgcgagtggg ggcagtcgaa gggcgaagcc cgcccgcctg ccccccgagc ctcacggcgg     2160

cgagtgcggg ggttccaagg gggcagcgcc accttgggca aggccgaagg ccgcgcagtc     2220

gatcaacaag ccccggaggg gccacttttt gccggagggg gagccgcgcc gaaggcgtgg     2280

gggaaccccg caggggtgcc cttctttggg caccaaagaa ctagatatag ggcgaaatgc     2340

gaaagactta aaaatcaaca acttaaaaaa ggggggtacg caacagctca ttgcggcacc     2400

ccccgcaata gctcattgcg taggttaaag aaaatctgta attgactgcc acttttacgc     2460

aacgcataat tgttgtcgcg ctgccgaaaa gttgcagctg attgcgcatg gtgccgcaac     2520

cgtgcggcac ccctaccgca tggagataag catggccacg cagtccagag aaatcggcat     2580

tcaagccaag aacaagcccg gtcactgggt gcaaacggaa cgcaaagcgc atgaggcgtg     2640

ggccgggctt attgcgagga agcccacggc ggcaatgctg ctgcatcacc tcgtggcgca     2700

gatgggccac cagaacgccg tggtggtcag ccagaaaaca ctttccaagc tcatcggacg     2760

ttctttgcgg acggtccaat acgcagtcaa ggacttggtg gccgagcgct ggatctccgt     2820

cgtgaagctc aacggccccg gcaccgtgtt ggcctacgtg gtcaatgacc gcgtggcgtg     2880

gggccagccc cgcgaccagt tgcgcctgtc ggtgttcagt gccgccgtgg tggttgatca     2940

cgacgaccag gacgaatcgc tgttggggca tggcgacctg cgccgcatcc cgaccctgta     3000

tccgggcgag cagcaactac cgaccggccc cggcgaggag ccgcccagcc agcccggcat     3060

tccgggcatg gaaccagacc tgccagcctt gaccgaaacg gaggaatggg aacggcgcgg     3120

gcagcagcgc ctgccgatgc ccgatgagcc gtgttttctg gacgatggcg agccgttgga     3180

gccgccgaca cgggtcacgc tgccgcgccg gtagcacttg ggttgcgcag caacccgtaa     3240

gtgcgctgtt ccagactatc ggctgtagcc gcctcgccgc cctatacctt gtctgcctcc     3300

ccgcgttgcg tcgcggtgca tggagccggg ccacctcgac ctgaatggaa gccggcggca     3360

cctcgctaac ggattcaccg ttactgcacg gcgttcggct gcggcgagcg gtatcagctc     3420

actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcaggc ctccgaaatc     3480

ctgcagggaa agccacgttg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa     3540

tatatcatca tgaacaataa aactgtctgc ttacataaac agtaatacaa ggggtgttat     3600

gagccatatt caacgggaaa cgtcttgctc gaggccgcga ttaaattcca acatggatgc     3660

tgatttatat gggtataaat gggctcgcga taatgtcggg caatcaggtg cgacaatcta     3720

tcgattgtat gggaagcccg atgcgccaga gttgtttctg aaacatggca aaggtagcgt     3780

tgccaatgat gttacagatg agatggtcag actaaactgg ctgacggaat ttatgcctct     3840

tccgaccatc aagcatttta tccgtactcc tgatgatgca tggttactca ccactgcgat     3900

ccccgggaaa acagcattcc aggtattaga agaatatcct gattcaggtg aaaatattgt     3960

tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta attgtccttt     4020

taac                                                                  4024


<210>  6
<211>  213
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SrpR repressor protein

<400>  6

Met Ala Arg Lys Thr Ala Ala Glu Ala Glu Glu Thr Arg Gln Arg Ile 
1               5                   10                  15      


Ile Asp Ala Ala Leu Glu Val Phe Val Ala Gln Gly Val Ser Asp Ala 
            20                  25                  30          


Thr Leu Asp Gln Ile Ala Arg Lys Ala Gly Val Thr Arg Gly Ala Val 
        35                  40                  45              


Tyr Trp His Phe Asn Gly Lys Leu Glu Val Leu Gln Ala Val Leu Ala 
    50                  55                  60                  


Ser Arg Gln His Pro Leu Glu Leu Asp Phe Thr Pro Asp Leu Gly Ile 
65                  70                  75                  80  


Glu Arg Ser Trp Glu Ala Val Val Val Ala Met Leu Asp Ala Val His 
                85                  90                  95      


Ser Pro Gln Ser Lys Gln Phe Ser Glu Ile Leu Ile Tyr Gln Gly Leu 
            100                 105                 110         


Asp Glu Ser Gly Leu Ile His Asn Arg Met Val Gln Ala Ser Asp Arg 
        115                 120                 125             


Phe Leu Gln Tyr Ile His Gln Val Leu Arg His Ala Val Thr Gln Gly 
    130                 135                 140                 


Glu Leu Pro Ile Asn Leu Asp Leu Gln Thr Ser Ile Gly Val Phe Lys 
145                 150                 155                 160 


Gly Leu Ile Thr Gly Leu Leu Tyr Glu Gly Leu Arg Ser Lys Asp Gln 
                165                 170                 175     


Gln Ala Gln Ile Ile Lys Val Ala Leu Gly Ser Phe Trp Ala Leu Leu 
            180                 185                 190         


Arg Glu Pro Pro Arg Phe Leu Leu Cys Glu Glu Ala Gln Ile Lys Gln 
        195                 200                 205             


Val Lys Ser Phe Glu 
    210             


<210>  7
<211>  639
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence encoding SrpR repressor protein

<400>  7
atggcacgta aaaccgcagc agaagcagaa gaaacccgtc agcgtattat tgatgcagca       60

ctggaagttt ttgttgcaca gggtgttagt gatgcaaccc tggatcagat tgcacgtaaa      120

gccggtgtta cccgtggtgc agtttattgg cattttaatg gtaaactgga agttctgcag      180

gcagttctgg caagccgtca gcatccgctg gaactggatt ttacaccgga tctgggtatt      240

gaacgtagct gggaagcagt tgttgttgca atgctggatg cagttcatag tccgcagagc      300

aaacagttta gcgaaattct gatttatcag ggtctggatg aaagcggtct gattcataat      360

cgtatggttc aggcaagcga tcgttttctg cagtatattc atcaggttct gcgtcatgca      420

gttacccagg gtgaactgcc gattaatctg gatctgcaga ccagcattgg tgtttttaaa      480

ggtctgatta ccggtctgct gtatgaaggt ctgcgtagca aagatcagca ggcacagatt      540

atcaaagttg cactgggtag cttttgggca ctgctgcgtg aaccgcctcg ttttctgctg      600

tgtgaagaag cacagattaa acaggtgaaa tccttcgaa                             639


<210>  8
<211>  67
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SrpR promoter-operator sequence

<400>  8
gattcgttac caattgacag ctagctcagt cctaggtata tacatacatg cttgtttgtt       60

tgtaaac                                                                 67


<210>  9
<211>  7393
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid p455 containing the Cpf1 nuclease under the control of 
       the PsrpR promoter, a crRNA targeting LacZ and a Lambda phage cos
       signal

<400>  9
gacagctttg aattgtttta ggggcgttat tcgagggcaa tcggagctaa cttcaagact       60

acttctttgt tgaatactaa atagtgcaaa ggtcgtgttt cctcaaggat actccgctaa      120

caatatagga ttccaatcag attcagcact ggcggtacgg gtgttgcggt gaggcgttcg      180

ggtttacggc tcgaagctag cacggtagga agcctgacaa tcaccaagca aaagggccgt      240

cgaaggccca caagatacga aagctctcga agccttatcc ttgaccgatc cacctattta      300

ggcagttacg cacaaaagct acccaataat ccgtgacagg cacaatatca cggaacaaaa      360

ccgaaaactc tcgtacacgg ttaggttttc gctaggaaga ataaacctct atcttgatta      420

taagaaggct ccccaagcac ccccaaaacc gaaatagcgg tttgcaataa gggacaagtt      480

acgagtgtag acacgcagaa ttatccagcc tttagtcttt aggaaggcaa agctattgta      540

cgcggtagcc gtcgtagcaa tttaccaact gtagaattat tggacacacg taggaagggc      600

ttacagttga agtttaataa ggtcacacgc aaaaccgcta aggaataatc gcaccgttag      660

cgaaagaata tttcagagcg gttagtaaag gttgagtaaa gtgagattcc aaagtgagcc      720

tttataaaaa gtaaagagct ataataaaac cgtcgagcag aaaacaatcg cctgaaatct      780

caagcacgtt gccctttcta acgtcgctaa ggtttcgtaa acccgtttga ttaggaagaa      840

gaataagtaa cccgattagg tttgagatcg cgggttatcg gtttggatta aaagtggata      900

ccagcggagt caacgccgac gcaaacgtac agtgatccaa tcctgttgca cggtcaagca      960

caatcagctc gcaagatctt ggaatagtgt gcccaacagt ttagttgagg gccacgttcc     1020

gactacaagt tgcttcaaga ggggaatttg gatttggcgg caaaagcacc gccggacatc     1080

agcgctagcg gagtgtatac tggcttacta tgttggcact gatgagggtg taagtgaagt     1140

gcttcatgtg gcaggagaaa aaaggctgca ccggtgcgtc agcagaatat gtgatacagg     1200

atatattccg cttcctcgct cactgactcg ctacgctcgg tcgttcgact gcggcgagcg     1260

gaaatggctt acgaacgggg cggagatttc ctggaagatg ccaggaagat acttaacagg     1320

gaagtgagag ggccgcggca aagccgtttt tccataggct ccgcccccct gacaagcatc     1380

acgaaatctg acgctcaaat cagtggtggc gaaacctgac aggactataa agataccagg     1440

cgtttccccc tggcggctcc ctcgtgcgct ctcctgttcc tgcctttcgg tttaccggtg     1500

tcattccgct gttatggccg cgtttgtctc attccacgcc tgacactcag ttccgggtag     1560

gcagttcgct ccaagctgga ctgtatgcac gaaccccccg ttcagtccga ccgctgcgcc     1620

ttatccggta actatcgtct tgagtccaac ccggaaagac atgcaaaagc accactggca     1680

gcagccactg gtaattgatt tagaggagtt agtcttgaag tcatgcgccg gataaggcta     1740

aactgaaagg acaagttttg gcgactgcgc tcctccaagc cagttacctc ggttcaaaga     1800

gttggtagct cagagaacct tcgaaaaacc gccctgcaag gcggtttttt cgttttcaga     1860

gcaagagatt acgcgcagac caaaacgatc tcaagaagat catcttatta atcagataaa     1920

atatttctag atttcagtgc aatttatctc ttcaaatgta gcacgcgacg tacggtggaa     1980

gattcgttac caattgacag ctagctcagt cctaggtata tacatacatg cttgtttgtt     2040

tgtaaactac tttcgagtac taacaaagct ggtttgaatg tccatctatc aggagtttgt     2100

taacaagtat tccctgtcta aaaccctgcg ttttgaactg atcccgcagg gcaaaacttt     2160

ggaaaacatt aaagcgcgtg gcctgattct ggatgacgaa aaacgtgcaa aggattacaa     2220

gaaagctaaa cagatcatcg acaaatatca ccagttcttt atcgaagaaa ttctgtcgtc     2280

ggtgtgcatc agtgaggatc tgttacagaa ttattctgat gtatacttta aacttaaaaa     2340

gtccgatgac gataatctgc aaaaagattt caagtcagcc aaagatacca tcaagaaaca     2400

gatctcagaa tatattaaag atagcgaaaa gttcaaaaac ctgtttaacc aaaacctcat     2460

tgatgctaag aaaggccaag aatctgacct gatcttatgg ctgaaacaga gcaaagataa     2520

cggcattgaa ctgttcaaag ctaatagcga catcaccgat attgatgaag cgctcgaaat     2580

catcaagtct ttcaaaggct ggacgacgta tttcaaaggt tttcatgaaa accgtaagaa     2640

tgtatattcg agcaacgata ttccgacctc tattatttat cgtatcgtgg acgacaacct     2700

gccgaagttt ctggaaaaca aagcgaaata tgaatctctg aaagacaaag caccggaagc     2760

tattaactat gaacagatca agaaagatct ggcggaagaa ctgaccttcg acatcgacta     2820

taaaacctcc gaagttaacc agcgtgtttt ctcactggac gaggttttcg aaatcgctaa     2880

tttcaacaat tacctgaatc aatctggcat caccaaattc aacaccatta ttggtggcaa     2940

atttgttaac ggcgaaaaca ccaagcgtaa gggcatcaac gaatacatta acctgtatag     3000

ccaacaaatc aacgacaaaa ccctgaaaaa gtataaaatg tccgttctgt ttaaacagat     3060

tttatcggac accgaatcta aatccttcgt aattgataaa ctggaagatg atagcgacgt     3120

tgtcaccacg atgcagagct tttatgagca gattgcggcg ttcaaaaccg tggaagagaa     3180

atctattaaa gaaactctgt ccctgctctt tgacgacctc aaagcgcaga aactagatct     3240

gtctaagatt tactttaaaa acgacaaatc tctgaccgat ctcagtcaac aagttttcga     3300

tgactatagc gtgatcggca cggcagtttt ggaatacatc acccaacaaa tcgcgccgaa     3360

aaatctggac aacccgtcca agaaggaaca ggaactgatt gcaaagaaaa cagaaaaagc     3420

taaatacctg agcttagaaa ctatcaaact ggcacttgag gaatttaata aacatcgtga     3480

tattgataaa cagtgtcgtt ttgaggaaat tctggcgaac tttgcggcaa tcccgatgat     3540

cttcgacgaa attgctcaaa acaaagacaa tctggcgcag atctctatca agtaccagaa     3600

tcagggtaag aaagatctgc ttcaagcatc tgcggaggac gatgtgaaag caattaaaga     3660

cttattagat cagacgaata acttattaca caagctcaaa atcttccaca tcagccagag     3720

cgaggacaag gcgaacattc tggataaaga tgaacacttc tatctggtgt tcgaagaatg     3780

ttacttcgaa ctggcaaaca tcgtccctct ctacaataaa atccgcaact acatcacgca     3840

gaagccttac tctgacgaga aattcaaact gaacttcgaa aacagcacgc tggcgaacgg     3900

ctgggataag aacaaagagc cggacaacac cgcaatcctg ttcatcaaag acgacaaata     3960

ctatctgggc gtaatgaaca agaagaacaa caagatcttc gacgataaag cgatcaaaga     4020

aaacaagggt gaaggctata agaaaatcgt gtacaagctc ctgccgggtg cgaataaaat     4080

gttaccgaaa gtgttctttt ccgcgaaaag catcaaattc tacaacccgt ctgaggatat     4140

tctgcgcatc cgcaatcata gcacgcacac taaaaacggt agcccgcaga aagggtatga     4200

aaaattcgaa tttaatatag aggactgccg taagttcatc gacttctata aacagagcat     4260

ttccaaacat ccggaatgga aagacttcgg cttccgtttc tctgacactc agcgctataa     4320

tagcatcgac gagttctacc gcgaagtgga gaatcagggc tataaactga ccttcgagaa     4380

cattagtgag tcgtacatcg actccgttgt gaatcagggt aaactgtacc tgtttcagat     4440

ctataataaa gactttagcg cgtacagcaa aggccgtccg aatctgcaca ccctttactg     4500

gaaagcatta tttgacgaac gtaacctgca agatgtggtg tataaactga acggtgaggc     4560

ggaacttttc taccgtaaac agagtatccc gaagaaaatc acgcatccgg caaaagaagc     4620

tattgccaac aaaaacaaag acaacccgaa gaaagaatca gtattcgaat atgacctgat     4680

caaagataaa cgtttcaccg aagataagtt ctttttccac tgtccgatta ccatcaactt     4740

caaatctagc ggtgcgaaca agttcaacga tgaaattaac ttattactga aagagaaagc     4800

taatgacgta cacatcttat ctattgatcg cggtgaacgt catttagcat actatacact     4860

ggtagatggt aaaggtaata ttattaaaca ggatactttc aatattatcg gtaatgaccg     4920

tatgaaaacc aactatcacg ataagctggc ggcgatcgaa aaagatcgtg attctgcgcg     4980

taaagattgg aagaaaatta acaatatcaa agaaatgaaa gaaggctatc tgagccaagt     5040

ggtgcacgag atcgcaaaac tggtgattga atataacgct atcgtggttt tcgaagatct     5100

gaactttggt tttaaacgtg gtcgcttcaa agtagaaaaa caggtgtacc aaaaactgga     5160

aaaaatgctg attgaaaaac tgaactatct ggtttttaaa gacaacgaat ttgacaaaac     5220

gggtggcgta ctccgtgcct atcagctgac cgctccgttc gaaacgttca agaaaatggg     5280

taaacaaacg gggattatct attatgtgcc agctggtttc acctccaaga tttgtccagt     5340

tacgggcttc gttaaccagc tgtacccgaa atacgagagc gttagcaaat ctcaagaatt     5400

tttcagcaaa ttcgacaaga tctgctataa tctggataaa ggctatttcg agttcagctt     5460

cgattacaaa aacttcggcg ataaagcggc taaaggtaag tggactattg ctagctttgg     5520

tagccgtctg attaactttc gcaactccga caaaaaccat aattgggaca cgcgtgaagt     5580

gtatccgacc aaagaactgg aaaaattact gaaagactat tccatcgaat atggtcatgg     5640

ggagtgcatt aaagcggcga tttgcggtga atccgataag aaatttttcg ccaaactgac     5700

cagcgtgctt aacaccattc tgcaaatgcg taattctaaa acgggtacgg agctggacta     5760

cctgatttct ccggtagccg acgttaacgg caacttcttc gattctcgtc aagcaccgaa     5820

aaatatgcca caagacgcgg atgccaacgg tgcataccat atcggcttaa aaggcttaat     5880

gttattaggc cgtatcaaga ataatcagga gggcaagaaa ttaaatctgg ttatcaaaaa     5940

cgaagaatac ttcgagttcg ttcagaatcg taacaattaa tgtatgctta agcagctcgg     6000

taccaaagac gaacaataag acgctgaaaa gcgtcttttt tcgttttggt cctgttgcgg     6060

cgcgatagtg tgaacatgct atagacttct ggtgctaccc gactgacaat taatcatccg     6120

gctcgtataa tgctagcaat ttctactgtt gtagatgcca gctggcgtaa tagcgaagag     6180

tcgagacgaa caataaggcc tccctaacgg ggggcctttt ttattgataa caaaagtaaa     6240

gccgaacgcc ccaaaaagcc tcgctttcag cacctgtcgt ttcctttctt ttcagagggt     6300

attttaaata aaaacattaa gttatgacga agaagaacgg aaacgcctta aaccggaaaa     6360

ttttcataaa tagcgaaaac ccgcgaggtc gccgccccgt aacctgtcgg atcaccggaa     6420

aggacccgta aagtgataat gattatcatc tacatatcac aacgtgcgta aagggactag     6480

tggattgcat acctttggtc gaaaaaaaaa gcccgcactg tcaggtgcgg gcttttttca     6540

gtgtttcctt gccggattac gccccgccct gccactcatc gcagtattgt tgtaattcat     6600

taagcattct gccgacatgg aagccatcac aaacggcatg atgaacttgg atcgccagtg     6660

gcattaacac cttgtcgcct tgcgtataat attttcccat agtgaaaacg ggggcgaaga     6720

agttgtccat atttgctacg tttaaatcaa aactggtgaa actcacccac ggattggcac     6780

tgacgaaaaa catattttcg ataaaccctt tagggaaata tgctaagttt tcaccgtaac     6840

acgccacatc ttgactatat atgtgtagaa actgccggaa atcgtcgtgg tattctgacc     6900

agagcgatga aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg tgaacactat     6960

cccatatcac cagctcaccg tctttcattg ccatacgaaa ctccggatgt gcattcatca     7020

ggcgggcaag aatgtgaata aaggccggat aaaacttgtg cttatttttc tttacggttt     7080

ttaaaaaggc cgtaatatcc agctgaacgg tttggttata ggtgcactga gcaactgact     7140

ggaatgcctc aaaatgttct ttacgatgcc attgacttat atcaactgta gtatatccag     7200

tgattttttt ctccatttta gcttccttag cttgcgaaat ctcgataact caaaaaatag     7260

tagtgatctt atttcattat ggtgaaagtt gtcttacgtg caacattttc gcaaaaagtt     7320

ggcgctttat caacactgtc cctcctgttc agctactgac ggtactgcgg aactgactaa     7380

agtagtgcgt aac                                                        7393


<210>  10
<211>  5119
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  plasmid encoding the SrpR repressor protein

<400>  10
gtaaaccagc aatagacata agcggctatt taacgaccct gccctgaacc gacgaccggg       60

tcatcgtggc cggatcttgc ggcccctcgg cttgaacgaa ttgttagaca ttatttgccg      120

actaccttgg tgatctcgcc tttcacgtag tggacaaatt cttccaactg atctgcgcgc      180

gaggccaagc gatcttcttc ttgtccaaga taagcctgtc tagcttcaag tatgacgggc      240

tgatactggg ccggcaggcg ctccattgcc cagtcggcag cgacatcctt cggcgcgatt      300

ttgccggtta ctgcgctgta ccaaatgcgg gacaacgtaa gcactacatt tcgctcatcg      360

ccagcccagt cgggcggcga gttccatagc gttaaggttt catttagcgc ctcaaataga      420

tcctgttcag gaaccggatc aaagagttcc tccgccgctg gacctaccaa ggcaacgcta      480

tgttctcttg cttttgtcag caagatagcc agatcaatgt cgatcgtggc tggctcgaag      540

atacctgcaa gaatgtcatt gcgctgccat tctccaaatt gcagttcgcg cttagctgga      600

taacgccacg gaatgatgtc gtcgtgcaca acaatggtga cttctacagc gcggagaatc      660

tcgctctctc caggggaagc cgaagtttcc aaaaggtcgt tgatcaaagc tcgccgcgtt      720

gtttcatcaa gccttacggt caccgtaacc agcaaatcaa tatcactgtg tggcttcagg      780

ccgccatcca ctgcggagcc gtacaaatgt acggccagca acgtcggttc gagatggcgc      840

tcgatgacgc caactacctc tgatagttga gtcgatactt cggcgatcac cgcttccctc      900

atctagtagg tttcctgtgt gactctagta ttattcgaag gatttcacct gtttaatctg      960

tgcttcttca cacagcagaa aacgaggcgg ttcacgcagc agtgcccaaa agctacccag     1020

tgcaactttg ataatctgtg cctgctgatc tttgctacgc agaccttcat acagcagacc     1080

ggtaatcaga cctttaaaaa caccaatgct ggtctgcaga tccagattaa tcggcagttc     1140

accctgggta actgcatgac gcagaacctg atgaatatac tgcagaaaac gatcgcttgc     1200

ctgaaccata cgattatgaa tcagaccgct ttcatccaga ccctgataaa tcagaatttc     1260

gctaaactgt ttgctctgcg gactatgaac tgcatccagc attgcaacaa caactgcttc     1320

ccagctacgt tcaataccca gatccggtgt aaaatccagt tccagcggat gctgacggct     1380

tgccagaact gcctgcagaa cttccagttt accattaaaa tgccaataaa ctgcaccacg     1440

ggtaacaccg gctttacgtg caatctgatc cagggttgca tcactaacac cctgtgcaac     1500

aaaaacttcc agtgctgcat caataatacg ctgacgggtt tcttctgctt ctgctgcggt     1560

tttacgtgcc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct     1620

catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagcta gctcactcgg     1680

tcgctacgct ccgggcgtga gactgcggcg ggcgctgcgg acacatacaa agttacccac     1740

agattccgtg gataagcagg ggactaacat gtgaggcaaa acagcagggc cgcgccggtg     1800

gcgtttttcc ataggctccg ccctcctgcc agagttcaca taaacagacg cttttccggt     1860

gcatctgtgg gagccgtgag gctcaaccat gaatctgaca gtacgggcga aacccgacag     1920

gacttaaaga tccccaccgt ttccggcggg tcgctccctc ttgcgctctc ctgttccgac     1980

cctgccgttt accggatacc tgttccgcct ttctccctta cgggaagtgt ggcgctttct     2040

catagctcac acactggtat ctcggctcgg tgtaggtcgt tcgctccaag ctgggctgta     2100

agcaagaact ccccgttcag cccgactgct gcgccttatc cggtaactgt tcacttgagt     2160

ccaacccgga aaagcacggt aaaacgccac tggcagcagc cattggtaac tgggagttcg     2220

cagaggattt gtttagctaa acacgcggtt gctcttgaag tgtgcgccaa agtccggcta     2280

cactggaagg acagatttgg ttgctgtgct ctgcgaaagc cagttaccac ggttaagcag     2340

ttccccaact gacttaacct tcgatcaaac cacctcccca ggtggttttt tcgtttacag     2400

ggcaaaagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc actcttcctt     2460

tttcaatatt attgaacctg aggtctgttt atcgaattaa ttgcagatat aaaaaaacca     2520

accgtaaggg ttggtttttt cttgggattt ttggtcggca cgagaggatt tgaacctccg     2580

acccccgaca ccccatgacg gtgcgctacc aggctgcgct acgtgccgac tcgtggctgc     2640

taatactacc gttttccaca ccgattgcaa gtaagatatt tcgctaactg atttataatt     2700

aatcagttag cgataaaacg cttctcgtac aacgctttct ggtgaatggt gcgggaggcg     2760

agacttgaac tcgcacacct tgcggcgcca gaacctaaat ctggtgcgtc taccaatttc     2820

gccactcccg caaaaaaaga tggtggctac gacgggattc gaacctgtga ccccatcatt     2880

atgagtgatg tgctctaacc aactgagcta cgtagccatc ttttttttcg cgatacctta     2940

tcggcgttgc ggggcgcatt atgcgtatag agccttgcag cgtcaacctc tttttcaagg     3000

aaaattgctc gaaagtgact gtttggttag gttgcgaaca gcgaaccatg acgaactgta     3060

aatctacgga atgcttgata ttcaggggat ttttgcggac tggtacggat gggagcgaac     3120

tgataaatgg tgtcccctgc aggaatcgaa cctgcaatta gcccttagga ggggctcgtt     3180

atatccattt aactaagagg acaatgcggc atgagtatac ccgctaatgg agtgcggggt     3240

aagtacgttg ccgctcgatt gcttaaaccc tcgccattta tgccgggttt ttataatttt     3300

tcttaatgtt ttccgcacgt tctgcttttt ggacgtcatc gattgtccct ctaagacacg     3360

gataaatcgg tgatatcacc acatcaacca ggcaacatgc ccgacttgtt gaatgcaata     3420

aacagaagga aaaaacaggg aggagaaaag aagtggtgct gataggcaga ttcgaactgc     3480

cgacctcacc cttaccaagg gtgcgctcta ccaactgagc tatatcagca catcttggag     3540

cgggcagcgg gaatcgaacc cgcatcatca gcttggaagg ctgaggtaat agccattata     3600

cgatgcccgc atcctggaac tcggctacct gattttcatt ctgcactgaa tatcgagaga     3660

agctctcttt attcgagccg gtaagcgaac ttatcgtctc gggctacgcc atcgcgtggc     3720

cgaaattggt ggtgggggaa ggattcgaac cttcgaagtc tgtgacggca gatttacagt     3780

ctgctccctt tggccgctcg ggaaccccac cggacttgat ggtgccgact accggaatcg     3840

aactggtgac ctactgatta caagtcagtt gctctaccta ctgagccaag tcggcatcaa     3900

gtagcgcgca ctctatggag acatgcgagt tcatgcaact aaaaaattgc ataatttgtt     3960

ttattggtca cattttatgc gacacgatga agaaacagcc taggtacctc atgagcccga     4020

agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac     4080

ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatccgt cacataccaa     4140

ggcggctaag cgagcagatg gaacatcaac gcctgcggtc aggaagatgc gcatcgacag     4200

caagacatca tggctatcag cttgtcgtcg tgcaggaatt gaagatttcc gtttccatga     4260

cctcagacac acctgggcaa gctggctgat tcagtcaggc gtcccattat cagtgcttca     4320

ggaaatgggc ggatgggagt ccatagaaat ggttcgtagg tatgctcacc ttgcgcctaa     4380

tcatttgaca gagcatgcga ggaaaataga cgacattttt ggtgataatg tcccaaatat     4440

gtcccactct gaaattatgg aggatataaa gaaggcgtaa ctgattgaat tgtaatggcg     4500

cgccctgcag gattcgaacc tgcggcccac gacttagaag gtcgttgctc tatccaactg     4560

agctaagggc gcgttgatac cgcaatgcgg tgtaatcgcg tgaattatac ggtcaaccct     4620

tgctgagtca atggcttttg atctggttgc tgaacaagtg aacgaccgcg tctgattttc     4680

tgatttattt cgctatagcg gcaaacaaac gcacaccgct gcgcgtctga atcaagaaaa     4740

cccgtatttt catgtatcaa agtgacctgc agccaagctt ggattgcgac acggagttac     4800

tttataatcc aatcgattgg ccccttagct cagtggttag agcaggcgac tcataatcgc     4860

ttggtcgctg cttcaagtcc agcaggggcc accagcggcc gcaaaggctg acgagaaatc     4920

gtcagccttt ttaagcttta agccgaattc cagcacactg gcggccgtta ctagtggatc     4980

cgagctcggt accaagctta tcgatgataa gctgtcaaac atgagaatta caacttatat     5040

cgtatggggc tgacttcagc tgaaacctca ggcatttgag aagcacacgg tcacactgct     5100

tccggtagtc aataaaccg                                                  5119


<210>  11
<211>  12335
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid comprising the PsrpR-Cpf1-LacZ circuit

<400>  11
ctaatctctt gcccccgccc gtaatagcct ccaagagatt gaagatagta aagggcaaga       60

gctgattcgg cgttgaagga tagcggactt tcggtcaacc acaattcccc actcgacaaa      120

accagccgtg cgaataactc tgaaagtaca agcaacccaa gagggctgag cctaaactca      180

gctaattcct aagtgagcta aagactcgaa gtgacagctc ttaataaata gagcgggaac      240

gtcgaacggt cgtgaaagta atagtacaac gggtattaac ttactgagga tattgcttga      300

agctgtaccg ttttattggg tgaacgaata agatccagca attcagccaa agaagctacc      360

aatttttagt ttaagagtgt cacgtctgac ctcgcgggta gattgccgaa cgtagagctt      420

acgagccagc ggaaacagta gccgcaggat aagtaagggg agtaagtgat cgaacgaatc      480

agaagtgaca atatacttag gctggatctc gtcccgtgaa tcccaaccct caccaactac      540

gagataagag gtaagccaaa aatcgacttg gtggcgacca acgactgttc cccccctgta      600

actaatcgtt ccgtcaaaac ctgacttact tcaaggccaa ttccaagcgc aaacaatacc      660

gtcctagttc ttcggttaag tttccgaagt aggagtgagc ctacctccgt ttgcgtcttg      720

ttaccactga cccagctatt tactttgtat tgcctgcaat cgaatttctg aactctcaga      780

tagtggggat aacgggaaag ttcctatatt tgcgaactaa cttagccgtc cacctcgaag      840

ctacctactc acacccaccc cgcgcggggt aaataaggca ctaatcccag ctgagagctg      900

gcgtagcact tagccacaag ttaattaaca gttgtctggt agtttggcgg tattaggaag      960

atcctagaag caaggcagag ttagttctaa cctaaagcca caaataagac aggttgccaa     1020

agcccgccgg aaattaaatc ttgctcagtt cggtaacgga gtttccctcc cgcgtactta     1080

attcccaata agaaacgcgc ccaagtccta tcaggcaaaa ttcagcccct tcccgtgtta     1140

gaacgagggt aaaaatacaa gccgattgaa caagggttgg gggcttcaaa tcgtcgttta     1200

ccccacttta caacggagat taagtagttc accctatagt acgaagcaga actatttcga     1260

ggggcgtgca ataatcgaat cttctgcggt tgacttaaca cgctagggac gtgccctcga     1320

ttcaatcgaa ggtactccta ctcagactgc ctcacaccca gctagtcact gagcgataaa     1380

attgacccgc cctctaggga agcgagtacg tcccaaaggg ctccggacag ggctatatag     1440

gagagtttga tctcgccccg acaactgcaa ccctcaactc ccttagataa tattgttagc     1500

cgaagttgca cgacccgccg tccacggact gctcttaggg tgtggctcct taatctgaca     1560

acgtgcaacc cctatcgaag tcgattgttt ctgcgaaagg tgttgtccta atagtcccga     1620

aatttggccc ttgtaggtgt gaaaccactt agcttcgcgc cgtagtccta aaggcccacc     1680

tattgacttt gtttcgggta gcactaggaa tcttaacaat ttgaatttgg acgtggaacg     1740

cgtacacctt aatctccgaa taattctagg gatttggaag tcctctacgt tgacacacct     1800

acactgctcg aagtaaatat acgaataacg cgggcctcgc ggagccgttc cgaatcgtca     1860

cgtgttcgtt tactgttaat tggtggcaaa taagcaatat cgtagtccgt caggcccagc     1920

cctgttatcc acggcgttat ttgtcaaatt gcgtagaact ggattgactg cctgacaata     1980

cctaattatc ggtacgaagt ccccgaatct gtcgggctat ttcactaata ctttccaaac     2040

gccccgtatc caagaagaac gaatttatcc acgctcccgt ctttgggacg aataccgcta     2100

caagtggaca gaggatcggt acgggcctct aataaatcca acactctacg ccctcttcaa     2160

gagctagaag aacagggtgc agttggaaag ggaattattt cgtaaggcga gccaataccg     2220

taattaattc ggaagagtta acacgattgg aagtaggaat agtttctaac cacggttact     2280

aatcctaata acggaacgct gtctgataga ttagtgtcag cgctcggtac caaagaaaaa     2340

taaaaagacg ctgaaaagcg tctttttatt tttcggtcca gtgtaactca ggcaaaagca     2400

cgtaatattc gtactttctt cctccgtaag cgtcacccac attccttaaa gagtgcatgt     2460

gcatattttg ttatcaataa aaaaggccgc gatttgcggc cttattgttc gtcttgccgg     2520

attacgcccc gccctgccac tcatcgcagt attgttgtaa ttcattaagc attctgccga     2580

catggaagcc atcacaaacg gcatgatgaa cttggatcgc cagtggcatt aacaccttgt     2640

cgccttgcgt ataatatttt cccatagtga aaacgggggc gaagaagttg tccatatttg     2700

ctacgtttaa atcaaaactg gtgaaactca cccacggatt ggcactgacg aaaaacatat     2760

tttcgataaa ccctttaggg aaatatgcta agttttcacc gtaacacgcc acatcttgac     2820

tatatatgtg tagaaactgc cggaaatcgt cgtggtattc tgaccagagc gatgaaaacg     2880

tttcagtttg ctcatggaaa acggtgtaac aagggtgaac actatcccat atcaccagct     2940

caccgtcttt cattgccata cgaaactccg gatgtgcatt catcaggcgg gcaagaatgt     3000

gaataaaggc cggataaaac ttgtgcttat ttttctttac ggtttttaaa aaggccgtaa     3060

tatccagctg aacggtttgg ttataggtgc actgagcaac tgactggaat gcctcaaaat     3120

gttctttacg atgccattga cttatatcaa ctgtagtata tccagtgatt tttttctcca     3180

ttttagcttc cttagcttgc gaaatctcga taactcaaaa aatagtagtg atcttatttc     3240

attatggtga aagttgtctt acgtgcaaca ttttcgcaaa aagttggcgc tttatcaaca     3300

ctgtcggaat gacaaatggt tccaattatt gaacaccctt cggggtgttt ttttgtttct     3360

ggtttcccga ggccggcctg cgctagcgga gtgtatactg gcttactatg ttggcactga     3420

tgagggtgta agtgaagtgc ttcatgtggc aggagaaaaa aggctgcatc ggtgcgtcag     3480

cagaatatgt gatacaggat atattccgct tcctcgctca ctgactcgct acgctcggtc     3540

gttcgactgt ggcgagcgga aatggcttac gaacggggcg gagatttcct ggaagatgcc     3600

aggaagatac ttaacaggga agtgagaggg tcgcggcaaa gccgtttttc cataggctcc     3660

gcccccctga caagcatcac gaaatctgac gctcaaatca gtggtggcga aacctgacag     3720

gactataaag ataccaggcg tttccccctg gcggctccct cgtgcgctct cctgttcctg     3780

cctttcggtt tgccggtgtc attcctctgt tacggccgag tttgtctcat tccacgcctg     3840

acactcagtt ccgggtaggc agttcgctcc aagctggact gtatgcacga accccccgtt     3900

cagtccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggaaagacat     3960

gcaaaagcac cactggcagc agccactggt aattgattta gaggagttag tcttgaagtc     4020

atgcgccgga taaggctaaa ctgaaaggac aagttttggc gactgcgctc ctccaagcca     4080

gttacctcgg ttcaaagagt tggtagctca gagaaccttc gaaaaaccgc cctgcaaggc     4140

ggttttttcg ttttcagagc aagagattac gcgcagacca aaacgatctc aagaagatca     4200

tcttattaat cagataaaat atttctagat ttcagtgcaa tttatctctt caaatgtagc     4260

accggcgcgc cgtgaccaat tattgaaggc cgctaacgcg gccttttttt gtttctggta     4320

tcccgaatgg agcgacttct ccccaaaaag cctcgctttc agcacctgtc gtttcctttc     4380

ttttcagagg gtattttaaa taaaaacatt aagttatgac gaagaagaac ggaaacgcct     4440

taaaccggaa aattttcata aatagcgaaa acccgcgagg tcgccgcccc gtaacctgtc     4500

ggatcaccgg aaaggacccg taaagtgata atgattatca tctacatatc acaacgtgcg     4560

taaagggtaa gtatgaaggt cgtgtactcc atcgctacca aattccagaa aacagacgct     4620

ttcgagcgtc ttttttcgtt ttggtcacga cgtacggtgg aagattcgtt accaattgac     4680

agctagctca gtcctaggta tatacataca tgcttgtttg tttgtaaact actgttttca     4740

ttaaagagga gaaaggaagc catgtccatc tatcaggagt ttgttaacaa gtattccctg     4800

tctaaaaccc tgcgttttga actgatcccg cagggcaaaa ctttggaaaa cattaaagcg     4860

cgtggcctga ttctggatga cgaaaaacgt gcaaaggatt acaagaaagc taaacagatc     4920

atcgacaaat atcaccagtt ctttatcgaa gaaattctgt cgtcggtgtg catcagtgag     4980

gatctgttac agaattattc tgatgtatac tttaaactta aaaagtccga tgacgataat     5040

ctgcaaaaag atttcaagtc agccaaagat accatcaaga aacagatctc agaatatatt     5100

aaagatagcg aaaagttcaa aaacctgttt aaccaaaacc tcattgatgc taagaaaggc     5160

caagaatctg acctgatctt atggctgaaa cagagcaaag ataacggcat tgaactgttc     5220

aaagctaata gcgacatcac cgatattgat gaagcgctcg aaatcatcaa gtctttcaaa     5280

ggctggacga cgtatttcaa aggttttcat gaaaaccgta agaatgtata ttcgagcaac     5340

gatattccga cctctattat ttatcgtatc gtggacgaca acctgccgaa gtttctggaa     5400

aacaaagcga aatatgaatc tctgaaagac aaagcaccgg aagctattaa ctatgaacag     5460

atcaagaaag atctggcgga agaactgacc ttcgacatcg actataaaac ctccgaagtt     5520

aaccagcgtg ttttctcact ggacgaggtt ttcgaaatcg ctaatttcaa caattacctg     5580

aatcaatctg gcatcaccaa attcaacacc attattggtg gcaaatttgt taacggcgaa     5640

aacaccaagc gtaagggcat caacgaatac attaacctgt atagccaaca aatcaacgac     5700

aaaaccctga aaaagtataa aatgtccgtt ctgtttaaac agattttatc ggacaccgaa     5760

tctaaatcct tcgtaattga taaactggaa gatgatagcg acgttgtcac cacgatgcag     5820

agcttttatg agcagattgc ggcgttcaaa accgtggaag agaaatctat taaagaaact     5880

ctgtccctgc tctttgacga cctcaaagcg cagaaactag atctgtctaa gatttacttt     5940

aaaaacgaca aatctctgac cgatctcagt caacaagttt tcgatgacta tagcgtgatc     6000

ggcacggcag ttttggaata catcacccaa caaatcgcgc cgaaaaatct ggacaacccg     6060

tccaagaagg aacaggaact gattgcaaag aaaacagaaa aagctaaata cctgagctta     6120

gaaactatca aactggcact tgaggaattt aataaacatc gtgatattga taaacagtgt     6180

cgttttgagg aaattctggc gaactttgcg gcaatcccga tgatcttcga cgaaattgct     6240

caaaacaaag acaatctggc gcagatctct atcaagtacc agaatcaggg taagaaagat     6300

ctgcttcaag catctgcgga ggacgatgtg aaagcaatta aagacttatt agatcagacg     6360

aataacttat tacacaagct caaaatcttc cacatcagcc agagcgagga caaggcgaac     6420

attctggata aagatgaaca cttctatctg gtgttcgaag aatgttactt cgaactggca     6480

aacatcgtcc ctctctacaa taaaatccgc aactacatca cgcagaagcc ttactctgac     6540

gagaaattca aactgaactt cgaaaacagc acgctggcga acggctggga taagaacaaa     6600

gagccggaca acaccgcaat cctgttcatc aaagacgaca aatactatct gggcgtaatg     6660

aacaagaaga acaacaagat cttcgacgat aaagcgatca aagaaaacaa gggtgaaggc     6720

tataagaaaa tcgtgtacaa gctcctgccg ggtgcgaata aaatgttacc gaaagtgttc     6780

ttttccgcga aaagcatcaa attctacaac ccgtctgagg atattctgcg catccgcaat     6840

catagcacgc acactaaaaa cggtagcccg cagaaagggt atgaaaaatt cgaatttaat     6900

atagaggact gccgtaagtt catcgacttc tataaacaga gcatttccaa acatccggaa     6960

tggaaagact tcggcttccg tttctctgac actcagcgct ataatagcat cgacgagttc     7020

taccgcgaag tggagaatca gggctataaa ctgaccttcg agaacattag tgagtcgtac     7080

atcgactccg ttgtgaatca gggtaaactg tacctgtttc agatctataa taaagacttt     7140

agcgcgtaca gcaaaggccg tccgaatctg cacacccttt actggaaagc attatttgac     7200

gaacgtaacc tgcaagatgt ggtgtataaa ctgaacggtg aggcggaact tttctaccgt     7260

aaacagagta tcccgaagaa aatcacgcat ccggcaaaag aagctattgc caacaaaaac     7320

aaagacaacc cgaagaaaga atcagtattc gaatatgacc tgatcaaaga taaacgtttc     7380

accgaagata agttcttttt ccactgtccg attaccatca acttcaaatc tagcggtgcg     7440

aacaagttca acgatgaaat taacttatta ctgaaagaga aagctaatga cgtacacatc     7500

ttatctattg atcgcggtga acgtcattta gcatactata cactggtaga tggtaaaggt     7560

aatattatta aacaggatac tttcaatatt atcggtaatg accgtatgaa aaccaactat     7620

cacgataagc tggcggcgat cgaaaaagat cgtgattctg cgcgtaaaga ttggaagaaa     7680

attaacaata tcaaagaaat gaaagaaggc tatctgagcc aagtggtgca cgagatcgca     7740

aaactggtga ttgaatataa cgctatcgtg gttttcgaag atctgaactt tggttttaaa     7800

cgtggtcgct tcaaagtaga aaaacaggtg taccaaaaac tggaaaaaat gctgattgaa     7860

aaactgaact atctggtttt taaagacaac gaatttgaca aaacgggtgg cgtactccgt     7920

gcctatcagc tgaccgctcc gttcgaaacg ttcaagaaaa tgggtaaaca aacggggatt     7980

atctattatg tgccagctgg tttcacctcc aagatttgtc cagttacggg cttcgttaac     8040

cagctgtacc cgaaatacga gagcgttagc aaatctcaag aatttttcag caaattcgac     8100

aagatctgct ataatctgga taaaggctat ttcgagttca gcttcgatta caaaaacttc     8160

ggcgataaag cggctaaagg taagtggact attgctagct ttggtagccg tctgattaac     8220

tttcgcaact ccgacaaaaa ccataattgg gacacgcgtg aagtgtatcc gaccaaagaa     8280

ctggaaaaat tactgaaaga ctattccatc gaatatggtc atggggagtg cattaaagcg     8340

gcgatttgcg gtgaatccga taagaaattt ttcgccaaac tgaccagcgt gcttaacacc     8400

attctgcaaa tgcgtaattc taaaacgggt acggagctgg actacctgat ttctccggta     8460

gccgacgtta acggcaactt cttcgattct cgtcaagcac cgaaaaatat gccacaagac     8520

gcggatgcca acggtgcata ccatatcggc ttaaaaggct taatgttatt aggccgtatc     8580

aagaataatc aggagggcaa gaaattaaat ctggttatca aaaacgaaga atacttcgag     8640

ttcgttcaga atcgtaacaa ttaatgtatg cttaagcagc tcggtaccaa agacgaacaa     8700

taagacgctg aaaagcgtct tttttcgttt tggtcctgtt gcggcgcgat agtgtgaaca     8760

tgctatagac ttctggtgct acccgactga caattaatca tccggctcgt ataatgctag     8820

caatttctac tgttgtagat cccgatgtac gcgcgcgtgg atgagtctaa gaactttaaa     8880

taatttgtct gtatattatt tcgagacgaa caataaggcc tccctaacgg ggggcctttt     8940

ttattgataa caaaagtaac ttcgagcttg tctacctcct agcaccatta ttgcaattaa     9000

taaacaacta acggacaatt ctacctaaca gttttcatat atgacgagca gttaagtgat     9060

gagtaaaggt gaggaattat ttactggtgt tgttccgatc ttagttgaac tggacggcga     9120

tgttaacggt cataaattca gtgttcgtgg tgaaggtgaa ggtgatgcaa ccaacggtaa     9180

gctgaccctg aaattcatct gcactactgg aaaattacca gtaccgtggc ctactctggt     9240

gactaccctg acctatggtg ttcagtgttt ttctcgttac cctgaccaca tgaagcaaca     9300

tgatttcttc aaatctgcaa tgccggaagg ttatgtacag gagcgcacca tttctttcaa     9360

agacgatggc acgtataaaa cccgtgcaga ggttaaattt gaaggtgaca ctctggtgaa     9420

tcgtattgaa ctgaaaggca ttgatttcaa agaggacggc aatattttag gccacaaact     9480

ggaatataac ttcaactccc ataacgttta catcaccgca gacaaacaga agaacggtat     9540

caaagctaac ttcaaaattc gccataacgt tgaagatggt agcgtacagc tggcggatca     9600

ttaccaacag aacactccga ttggagatgc tcctgtttta ctgccggata accactacct     9660

gtccacccag tctaaactgt cgaaggatcc gaacgaaaag cgcgaccaca tggtgttatt     9720

agagttcgtt accgctagtg gtatcacgca cggtatggat gaactctaca aataagacga     9780

acaataaggg gagcgggaaa ccgctcccct tttttattga taacaaaagt aaattgcacg     9840

ctgatagtct cccaattgcg aaggaccaaa acgaaaaaac accctttcgg gtgtcttttc     9900

tggaatttgg taccgagtac taggtatcgt gtaagtagcg aaggcccgta cgcgagataa     9960

actgctaggc aaccgcgact ctacgactgg tgctcgattt aatttcgctg acgtaaagaa    10020

attatcggca gtgcgtcaac tgccgtatct ttatcttaat taggtagttg gacaagccct    10080

tgaaagaaat agcaagagcc tgcctctcta ttgaagtcac ggcgaaagtc gggtagaaat    10140

caaagaaagc agaaattaaa tcggagtaac actaaggtgg gataactccg taactgacta    10200

cgcctttctc tagactttac ttgaccagat acactgtctt tgacacgttg aaggattaga    10260

gcaatcaaat ccaagactgg ctaagcacga agcaactctt gagtgttaaa aagttatctc    10320

ctgtattcgg gaagcgggta ctagaagatt gcagggactc cgacgttaag taaattacaa    10380

agtaataagt atcgttcagg atcacgttac cgcaataaga agcgagaata atataatttc    10440

cgaagtgctt accccagtag tgactattcc tataaccctt ctgagtgtcc ggaggcggaa    10500

atttgccacg aaagagaaag tatttccccg acaataataa aggggcgctc ctcagctttt    10560

ccacttggtt gggtaagcta ggcaactctg aaaggagttt cggcgaattg aagccgacag    10620

ctttgaattg ttttaggggc gttattcgag ggcaatcgga gctaacttca agactacttc    10680

tttgttgaat actaaatagt gcaaaggtcg tgtttcctca aggatactcc gctaacaata    10740

taggattcca atcagattca gcactggcgg tacgggtgtt gcggtgaggc gttcgggttt    10800

acggctcgaa gctagcacgg taggaagcct gacaatcacc aagcaaaagg gccgtcgaag    10860

gcccacaaga tacgaaagct ctcgaagcct tatccttgac cgatccacct atttaggcag    10920

ttacgcacaa aagctaccca ataatccgtg acaggcacaa tatcacggaa caaaaccgaa    10980

aactctcgta cacggttagg ttttcgctag gaagaataaa cctctatctt gattataaga    11040

aggctcccca agcaccccca aaaccgaaat agcggtttgc aataagggac aagttacgag    11100

tgtagacacg cagaattatc cagcctttag tctttaggaa ggcaaagcta ttgtacgcgg    11160

tagccgtcgt agcaatttac caactgtaga attattggac acacgtagga agggcttaca    11220

gttgaagttt aataaggtca cacgcaaaac cgctaaggaa taatcgcacc gttagcgaaa    11280

gaatatttca gagcggttag taaaggttga gtaaagtgag attccaaagt gagcctttat    11340

aaaaagtaaa gagctataat aaaaccgtcg agcagaaaac aatcgcctga aatctcaagc    11400

acgttgccct ttctaacgtc gctaaggttt cgtaaacccg tttgattagg aagaagaata    11460

agtaacccga ttaggtttga gatcgcgggt tatcggtttg gattaaaagt ggataccagc    11520

ggagtcaacg ccgacgcaaa cgtacagtga tccaatcctg ttgcacggtc aagcacaatc    11580

agctcgcaag atcttggaat agtgtgccca acagtttagt tgagggccac gttccgacta    11640

caagttgctt caagagggga atttggattt ggcaatagcc ccccgtttct acctcaagag    11700

gcgacgagta ttaaccgcgc cagctgtcgg cacaagggcc aaagaagatt ccaatttctt    11760

attcccgaat aacctccgaa tccctgcggg aaaatcaccg accgaatagc ctagaagcaa    11820

gggggaacag ataggtataa ttagcttaag agagtaccag ccgtgacaac agcgtagtaa    11880

ccacaaactt acgctggggc ttctttggcg gatttttaca gatactaaca aggtgatttg    11940

aagtacctta gttgaggatt taaacgcgct atccggtaat ctccaaattg ggaaataccg    12000

ttcaaagagg gctagaatta cttaaaagcc ttcacaccgc ctgcgctata cgcgcccact    12060

ctcccgttta tccgtccaag cggaagcagg gcgatcctcc gctaagatat tcttacgtgt    12120

aacgtagcta agtatcccaa atagctggcg tacgcgttga acaccgccta gaggatcgtg    12180

actcgccgga cgagcgtgtt attggggact tacgccagcg tagactacaa cgcgcccaga    12240

ttaaccctgc acgtattgcc ttgaataacg tactaatctc tccggctctc gacaatctat    12300

cgagcgactc gattatcaac gggtgtcttg cagtt                               12335


