                         SEQUENCE LISTING

<110>  CRISPR THERAPEUTICS AG
       BAYER HEALTHCARE, LLC
 
<120>  NOVEL HIGH FIDELITY RNA-PROGRAMMABLE ENDONUCLEASE SYSTEMS AND 
       USES THEREOF

<130>  CBTN-032PC

<140>  PCT/US2020/022394
<141>  2020-03-12

<150>  US 62/817,514
<151>  2019-03-12

<160>  133   

<170>  PatentIn version 3.5

<210>  1
<211>  1053
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<223>  The sequence CANNOT be where X equals the following combination -
       406: E, 412: D, 416: A, and 420: R

<220>
<221>  misc_feature
<222>  (1)..(1053)
<223>  M-SauCas9_X

<220>
<221>  misc_feature
<222>  (406)..(406)
<223>  X can be any of these amino acids: E, G,S,T,A, D

<220>
<221>  misc_feature
<222>  (412)..(412)
<223>  X can be any of these amino acids: D, G,S,T,A, E

<220>
<221>  misc_feature
<222>  (416)..(416)
<223>  X can be any of these amino acids: A, G, S, T, D, E

<220>
<221>  misc_feature
<222>  (420)..(420)
<223>  X can be any of these amino acids: R, A, G, S, T, D, E

<400>  1

Met Lys Arg Asn Tyr Ile Leu Gly Leu Asp Ile Gly Ile Thr Ser Val 
1               5                   10                  15      


Gly Tyr Gly Ile Ile Asp Tyr Glu Thr Arg Asp Val Ile Asp Ala Gly 
            20                  25                  30          


Val Arg Leu Phe Lys Glu Ala Asn Val Glu Asn Asn Glu Gly Arg Arg 
        35                  40                  45              


Ser Lys Arg Gly Ala Arg Arg Leu Lys Arg Arg Arg Arg His Arg Ile 
    50                  55                  60                  


Gln Arg Val Lys Lys Leu Leu Phe Asp Tyr Asn Leu Leu Thr Asp His 
65                  70                  75                  80  


Ser Glu Leu Ser Gly Ile Asn Pro Tyr Glu Ala Arg Val Lys Gly Leu 
                85                  90                  95      


Ser Gln Lys Leu Ser Glu Glu Glu Phe Ser Ala Ala Leu Leu His Leu 
            100                 105                 110         


Ala Lys Arg Arg Gly Val His Asn Val Asn Glu Val Glu Glu Asp Thr 
        115                 120                 125             


Gly Asn Glu Leu Ser Thr Lys Glu Gln Ile Ser Arg Asn Ser Lys Ala 
    130                 135                 140                 


Leu Glu Glu Lys Tyr Val Ala Glu Leu Gln Leu Glu Arg Leu Lys Lys 
145                 150                 155                 160 


Asp Gly Glu Val Arg Gly Ser Ile Asn Arg Phe Lys Thr Ser Asp Tyr 
                165                 170                 175     


Val Lys Glu Ala Lys Gln Leu Leu Lys Val Gln Lys Ala Tyr His Gln 
            180                 185                 190         


Leu Asp Gln Ser Phe Ile Asp Thr Tyr Ile Asp Leu Leu Glu Thr Arg 
        195                 200                 205             


Arg Thr Tyr Tyr Glu Gly Pro Gly Glu Gly Ser Pro Phe Gly Trp Lys 
    210                 215                 220                 


Asp Ile Lys Glu Trp Tyr Glu Met Leu Met Gly His Cys Thr Tyr Phe 
225                 230                 235                 240 


Pro Glu Glu Leu Arg Ser Val Lys Tyr Ala Tyr Asn Ala Asp Leu Tyr 
                245                 250                 255     


Asn Ala Leu Asn Asp Leu Asn Asn Leu Val Ile Thr Arg Asp Glu Asn 
            260                 265                 270         


Glu Lys Leu Glu Tyr Tyr Glu Lys Phe Gln Ile Ile Glu Asn Val Phe 
        275                 280                 285             


Lys Gln Lys Lys Lys Pro Thr Leu Lys Gln Ile Ala Lys Glu Ile Leu 
    290                 295                 300                 


Val Asn Glu Glu Asp Ile Lys Gly Tyr Arg Val Thr Ser Thr Gly Lys 
305                 310                 315                 320 


Pro Glu Phe Thr Asn Leu Lys Val Tyr His Asp Ile Lys Asp Ile Thr 
                325                 330                 335     


Ala Arg Lys Glu Ile Ile Glu Asn Ala Glu Leu Leu Asp Gln Ile Ala 
            340                 345                 350         


Lys Ile Leu Thr Ile Tyr Gln Ser Ser Glu Asp Ile Gln Glu Glu Leu 
        355                 360                 365             


Thr Asn Leu Asn Ser Glu Leu Thr Gln Glu Glu Ile Glu Gln Ile Ser 
    370                 375                 380                 


Asn Leu Lys Gly Tyr Thr Gly Thr His Asn Leu Ser Leu Lys Ala Ile 
385                 390                 395                 400 


Asn Leu Ile Leu Asp Xaa Leu Trp His Thr Asn Xaa Asn Gln Ile Xaa 
                405                 410                 415     


Ile Phe Asn Xaa Leu Lys Leu Val Pro Lys Lys Val Asp Leu Ser Gln 
            420                 425                 430         


Gln Lys Glu Ile Pro Thr Thr Leu Val Asp Asp Phe Ile Leu Ser Pro 
        435                 440                 445             


Val Val Lys Arg Ser Phe Ile Gln Ser Ile Lys Val Ile Asn Ala Ile 
    450                 455                 460                 


Ile Lys Lys Tyr Gly Leu Pro Asn Asp Ile Ile Ile Glu Leu Ala Arg 
465                 470                 475                 480 


Glu Lys Asn Ser Lys Asp Ala Gln Lys Met Ile Asn Glu Met Gln Lys 
                485                 490                 495     


Arg Asn Arg Gln Thr Asn Glu Arg Ile Glu Glu Ile Ile Arg Thr Thr 
            500                 505                 510         


Gly Lys Glu Asn Ala Lys Tyr Leu Ile Glu Lys Ile Lys Leu His Asp 
        515                 520                 525             


Met Gln Glu Gly Lys Cys Leu Tyr Ser Leu Glu Ala Ile Pro Leu Glu 
    530                 535                 540                 


Asp Leu Leu Asn Asn Pro Phe Asn Tyr Glu Val Asp His Ile Ile Pro 
545                 550                 555                 560 


Arg Ser Val Ser Phe Asp Asn Ser Phe Asn Asn Lys Val Leu Val Lys 
                565                 570                 575     


Gln Glu Glu Asn Ser Lys Lys Gly Asn Arg Thr Pro Phe Gln Tyr Leu 
            580                 585                 590         


Ser Ser Ser Asp Ser Lys Ile Ser Tyr Glu Thr Phe Lys Lys His Ile 
        595                 600                 605             


Leu Asn Leu Ala Lys Gly Lys Gly Arg Ile Ser Lys Thr Lys Lys Glu 
    610                 615                 620                 


Tyr Leu Leu Glu Glu Arg Asp Ile Asn Arg Phe Ser Val Gln Lys Asp 
625                 630                 635                 640 


Phe Ile Asn Arg Asn Leu Val Asp Thr Arg Tyr Ala Thr Arg Gly Leu 
                645                 650                 655     


Met Asn Leu Leu Arg Ser Tyr Phe Arg Val Asn Asn Leu Asp Val Lys 
            660                 665                 670         


Val Lys Ser Ile Asn Gly Gly Phe Thr Ser Phe Leu Arg Arg Lys Trp 
        675                 680                 685             


Lys Phe Lys Lys Glu Arg Asn Lys Gly Tyr Lys His His Ala Glu Asp 
    690                 695                 700                 


Ala Leu Ile Ile Ala Asn Ala Asp Phe Ile Phe Lys Glu Trp Lys Lys 
705                 710                 715                 720 


Leu Asp Lys Ala Lys Lys Val Met Glu Asn Gln Met Phe Glu Glu Lys 
                725                 730                 735     


Gln Ala Glu Ser Met Pro Glu Ile Glu Thr Glu Gln Glu Tyr Lys Glu 
            740                 745                 750         


Ile Phe Ile Thr Pro His Gln Ile Lys His Ile Lys Asp Phe Lys Asp 
        755                 760                 765             


Tyr Lys Tyr Ser His Arg Val Asp Lys Lys Pro Asn Arg Glu Leu Ile 
    770                 775                 780                 


Asn Asp Thr Leu Tyr Ser Thr Arg Lys Asp Asp Lys Gly Asn Thr Leu 
785                 790                 795                 800 


Ile Val Asn Asn Leu Asn Gly Leu Tyr Asp Lys Asp Asn Asp Lys Leu 
                805                 810                 815     


Lys Lys Leu Ile Asn Lys Ser Pro Glu Lys Leu Leu Met Tyr His His 
            820                 825                 830         


Asp Pro Gln Thr Tyr Gln Lys Leu Lys Leu Ile Met Glu Gln Tyr Gly 
        835                 840                 845             


Asp Glu Lys Asn Pro Leu Tyr Lys Tyr Tyr Glu Glu Thr Gly Asn Tyr 
    850                 855                 860                 


Leu Thr Lys Tyr Ser Lys Lys Asp Asn Gly Pro Val Ile Lys Lys Ile 
865                 870                 875                 880 


Lys Tyr Tyr Gly Asn Lys Leu Asn Ala His Leu Asp Ile Thr Asp Asp 
                885                 890                 895     


Tyr Pro Asn Ser Arg Asn Lys Val Val Lys Leu Ser Leu Lys Pro Tyr 
            900                 905                 910         


Arg Phe Asp Val Tyr Leu Asp Asn Gly Val Tyr Lys Phe Val Thr Val 
        915                 920                 925             


Lys Asn Leu Asp Val Ile Lys Lys Glu Asn Tyr Tyr Glu Val Asn Ser 
    930                 935                 940                 


Lys Cys Tyr Glu Glu Ala Lys Lys Leu Lys Lys Ile Ser Asn Gln Ala 
945                 950                 955                 960 


Glu Phe Ile Ala Ser Phe Tyr Asn Asn Asp Leu Ile Lys Ile Asn Gly 
                965                 970                 975     


Glu Leu Tyr Arg Val Ile Gly Val Asn Asn Asp Leu Leu Asn Arg Ile 
            980                 985                 990         


Glu Val Asn Met Ile Asp Ile Thr  Tyr Arg Glu Tyr Leu  Glu Asn Met 
        995                 1000                 1005             


Asn Asp  Lys Arg Pro Pro Arg  Ile Ile Lys Thr Ile  Ala Ser Lys 
    1010                 1015                 1020             


Thr Gln  Ser Ile Lys Lys Tyr  Ser Thr Asp Ile Leu  Gly Asn Leu 
    1025                 1030                 1035             


Tyr Glu  Val Lys Ser Lys Lys  His Pro Gln Ile Ile  Lys Lys Gly 
    1040                 1045                 1050             


<210>  2
<211>  1056
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<223>  The sequence CANNOT be where X equals the following combination -
       408: E, 414: R, 418: E, 422: H, 239: C, and 401: C

<220>
<221>  misc_feature
<222>  (1)..(1056)
<223>  M-SluCas9_x

<220>
<221>  misc_feature
<222>  (239)..(239)
<223>  X can be any of these amino acids:  C,  S, A

<220>
<221>  misc_feature
<222>  (401)..(401)
<223>  X can be any of these amino acids: C,  S, A

<220>
<221>  misc_feature
<222>  (408)..(408)
<223>  X can be any of these amino acids:  E, G,S,T,A,D

<220>
<221>  misc_feature
<222>  (414)..(414)
<223>  X can be any of these amino acids:  R, G,S,T,A, D, E

<220>
<221>  misc_feature
<222>  (418)..(418)
<223>  X can be any of these amino acids:  E, G, S, T, A, D

<220>
<221>  misc_feature
<222>  (422)..(422)
<223>  X can be any of these amino acids:  H,  A, G, S, T, D, E

<400>  2

Met Asn Gln Lys Phe Ile Leu Gly Leu Asp Ile Gly Ile Thr Ser Val 
1               5                   10                  15      


Gly Tyr Gly Leu Ile Asp Tyr Glu Thr Lys Asn Ile Ile Asp Ala Gly 
            20                  25                  30          


Val Arg Leu Phe Pro Glu Ala Asn Val Glu Asn Asn Glu Gly Arg Arg 
        35                  40                  45              


Ser Lys Arg Gly Ser Arg Arg Leu Lys Arg Arg Arg Ile His Arg Leu 
    50                  55                  60                  


Glu Arg Val Lys Lys Leu Leu Glu Asp Tyr Asn Leu Leu Asp Gln Ser 
65                  70                  75                  80  


Gln Ile Pro Gln Ser Thr Asn Pro Tyr Ala Ile Arg Val Lys Gly Leu 
                85                  90                  95      


Ser Glu Ala Leu Ser Lys Asp Glu Leu Val Ile Ala Leu Leu His Ile 
            100                 105                 110         


Ala Lys Arg Arg Gly Ile His Lys Ile Asp Val Ile Asp Ser Asn Asp 
        115                 120                 125             


Asp Val Gly Asn Glu Leu Ser Thr Lys Glu Gln Leu Asn Lys Asn Ser 
    130                 135                 140                 


Lys Leu Leu Lys Asp Lys Phe Val Cys Gln Ile Gln Leu Glu Arg Met 
145                 150                 155                 160 


Asn Glu Gly Gln Val Arg Gly Glu Lys Asn Arg Phe Lys Thr Ala Asp 
                165                 170                 175     


Ile Ile Lys Glu Ile Ile Gln Leu Leu Asn Val Gln Lys Asn Phe His 
            180                 185                 190         


Gln Leu Asp Glu Asn Phe Ile Asn Lys Tyr Ile Glu Leu Val Glu Met 
        195                 200                 205             


Arg Arg Glu Tyr Phe Glu Gly Pro Gly Lys Gly Ser Pro Tyr Gly Trp 
    210                 215                 220                 


Glu Gly Asp Pro Lys Ala Trp Tyr Glu Thr Leu Met Gly His Xaa Thr 
225                 230                 235                 240 


Tyr Phe Pro Asp Glu Leu Arg Ser Val Lys Tyr Ala Tyr Ser Ala Asp 
                245                 250                 255     


Leu Phe Asn Ala Leu Asn Asp Leu Asn Asn Leu Val Ile Gln Arg Asp 
            260                 265                 270         


Gly Leu Ser Lys Leu Glu Tyr His Glu Lys Tyr His Ile Ile Glu Asn 
        275                 280                 285             


Val Phe Lys Gln Lys Lys Lys Pro Thr Leu Lys Gln Ile Ala Asn Glu 
    290                 295                 300                 


Ile Asn Val Asn Pro Glu Asp Ile Lys Gly Tyr Arg Ile Thr Lys Ser 
305                 310                 315                 320 


Gly Lys Pro Gln Phe Thr Glu Phe Lys Leu Tyr His Asp Leu Lys Ser 
                325                 330                 335     


Val Leu Phe Asp Gln Ser Ile Leu Glu Asn Glu Asp Val Leu Asp Gln 
            340                 345                 350         


Ile Ala Glu Ile Leu Thr Ile Tyr Gln Asp Lys Asp Ser Ile Lys Ser 
        355                 360                 365             


Lys Leu Thr Glu Leu Asp Ile Leu Leu Asn Glu Glu Asp Lys Glu Asn 
    370                 375                 380                 


Ile Ala Gln Leu Thr Gly Tyr Thr Gly Thr His Arg Leu Ser Leu Lys 
385                 390                 395                 400 


Xaa Ile Arg Leu Val Leu Glu Xaa Gln Trp Tyr Ser Ser Xaa Asn Gln 
                405                 410                 415     


Met Xaa Ile Phe Thr Xaa Leu Asn Ile Lys Pro Lys Lys Ile Asn Leu 
            420                 425                 430         


Thr Ala Ala Asn Lys Ile Pro Lys Ala Met Ile Asp Glu Phe Ile Leu 
        435                 440                 445             


Ser Pro Val Val Lys Arg Thr Phe Gly Gln Ala Ile Asn Leu Ile Asn 
    450                 455                 460                 


Lys Ile Ile Glu Lys Tyr Gly Val Pro Glu Asp Ile Ile Ile Glu Leu 
465                 470                 475                 480 


Ala Arg Glu Asn Asn Ser Lys Asp Lys Gln Lys Phe Ile Asn Glu Met 
                485                 490                 495     


Gln Lys Lys Asn Glu Asn Thr Arg Lys Arg Ile Asn Glu Ile Ile Gly 
            500                 505                 510         


Lys Tyr Gly Asn Gln Asn Ala Lys Arg Leu Val Glu Lys Ile Arg Leu 
        515                 520                 525             


His Asp Glu Gln Glu Gly Lys Cys Leu Tyr Ser Leu Glu Ser Ile Pro 
    530                 535                 540                 


Leu Glu Asp Leu Leu Asn Asn Pro Asn His Tyr Glu Val Asp His Ile 
545                 550                 555                 560 


Ile Pro Arg Ser Val Ser Phe Asp Asn Ser Tyr His Asn Lys Val Leu 
                565                 570                 575     


Val Lys Gln Ser Glu Asn Ser Lys Lys Ser Asn Leu Thr Pro Tyr Gln 
            580                 585                 590         


Tyr Phe Asn Ser Gly Lys Ser Lys Leu Ser Tyr Asn Gln Phe Lys Gln 
        595                 600                 605             


His Ile Leu Asn Leu Ser Lys Ser Gln Asp Arg Ile Ser Lys Lys Lys 
    610                 615                 620                 


Lys Glu Tyr Leu Leu Glu Glu Arg Asp Ile Asn Lys Phe Glu Val Gln 
625                 630                 635                 640 


Lys Glu Phe Ile Asn Arg Asn Leu Val Asp Thr Arg Tyr Ala Thr Arg 
                645                 650                 655     


Glu Leu Thr Asn Tyr Leu Lys Ala Tyr Phe Ser Ala Asn Asn Met Asn 
            660                 665                 670         


Val Lys Val Lys Thr Ile Asn Gly Ser Phe Thr Asp Tyr Leu Arg Lys 
        675                 680                 685             


Val Trp Lys Phe Lys Lys Glu Arg Asn His Gly Tyr Lys His His Ala 
    690                 695                 700                 


Glu Asp Ala Leu Ile Ile Ala Asn Ala Asp Phe Leu Phe Lys Glu Asn 
705                 710                 715                 720 


Lys Lys Leu Lys Ala Val Asn Ser Val Leu Glu Lys Pro Glu Ile Glu 
                725                 730                 735     


Thr Lys Gln Leu Asp Ile Gln Val Asp Ser Glu Asp Asn Tyr Ser Glu 
            740                 745                 750         


Met Phe Ile Ile Pro Lys Gln Val Gln Asp Ile Lys Asp Phe Arg Asn 
        755                 760                 765             


Phe Lys Tyr Ser His Arg Val Asp Lys Lys Pro Asn Arg Gln Leu Ile 
    770                 775                 780                 


Asn Asp Thr Leu Tyr Ser Thr Arg Lys Lys Asp Asn Ser Thr Tyr Ile 
785                 790                 795                 800 


Val Gln Thr Ile Lys Asp Ile Tyr Ala Lys Asp Asn Thr Thr Leu Lys 
                805                 810                 815     


Lys Gln Phe Asp Lys Ser Pro Glu Lys Phe Leu Met Tyr Gln His Asp 
            820                 825                 830         


Pro Arg Thr Phe Glu Lys Leu Glu Val Ile Met Lys Gln Tyr Ala Asn 
        835                 840                 845             


Glu Lys Asn Pro Leu Ala Lys Tyr His Glu Glu Thr Gly Glu Tyr Leu 
    850                 855                 860                 


Thr Lys Tyr Ser Lys Lys Asn Asn Gly Pro Ile Val Lys Ser Leu Lys 
865                 870                 875                 880 


Tyr Ile Gly Asn Lys Leu Gly Ser His Leu Asp Val Thr His Gln Phe 
                885                 890                 895     


Lys Ser Ser Thr Lys Lys Leu Val Lys Leu Ser Ile Lys Pro Tyr Arg 
            900                 905                 910         


Phe Asp Val Tyr Leu Thr Asp Lys Gly Tyr Lys Phe Ile Thr Ile Ser 
        915                 920                 925             


Tyr Leu Asp Val Leu Lys Lys Asp Asn Tyr Tyr Tyr Ile Pro Glu Gln 
    930                 935                 940                 


Lys Tyr Asp Lys Leu Lys Leu Gly Lys Ala Ile Asp Lys Asn Ala Lys 
945                 950                 955                 960 


Phe Ile Ala Ser Phe Tyr Lys Asn Asp Leu Ile Lys Leu Asp Gly Glu 
                965                 970                 975     


Ile Tyr Lys Ile Ile Gly Val Asn Ser Asp Thr Arg Asn Met Ile Glu 
            980                 985                 990         


Leu Asp Leu Pro Asp Ile Arg Tyr  Lys Glu Tyr Cys Glu  Leu Asn Asn 
        995                 1000                 1005             


Ile Lys  Gly Glu Pro Arg Ile  Lys Lys Thr Ile Gly  Lys Lys Val 
    1010                 1015                 1020             


Asn Ser  Ile Glu Lys Leu Thr  Thr Asp Val Leu Gly  Asn Val Phe 
    1025                 1030                 1035             


Thr Asn  Thr Gln Tyr Thr Lys  Pro Gln Leu Leu Phe  Lys Arg Gly 
    1040                 1045                 1050             


Asn Gly  Gly 
    1055     


<210>  3
<211>  1054
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<223>  The sequence CANNOT be where X equals the following combination -
       408:E, 414: R, 418: E, 422: Y, 239: C, and 401:C

<220>
<221>  misc_feature
<222>  (1)..(1054)
<223>  M-SpaCas9_X

<220>
<221>  misc_feature
<222>  (239)..(239)
<223>  X can be any of these amino acids:  C,  S, A

<220>
<221>  misc_feature
<222>  (401)..(401)
<223>  X can be any of these amino acids:  C,  S, A

<220>
<221>  misc_feature
<222>  (408)..(408)
<223>  X can be any of these amino acids:  E, G,S,T,A,D

<220>
<221>  misc_feature
<222>  (414)..(414)
<223>  X can be any of these amino acids:  R, G,S,T,A, D, E

<220>
<221>  misc_feature
<222>  (418)..(418)
<223>  X can be any of these amino acids:  E, G, S, T, A, D

<220>
<221>  misc_feature
<222>  (422)..(422)
<223>  X can be any of these amino acids:  Y, A, G, S, T, D, E

<400>  3

Met Lys Glu Lys Tyr Ile Leu Gly Leu Asp Leu Gly Ile Thr Ser Val 
1               5                   10                  15      


Gly Tyr Gly Ile Ile Asn Phe Glu Thr Lys Lys Ile Ile Asp Ala Gly 
            20                  25                  30          


Val Arg Leu Phe Pro Glu Ala Asn Val Asp Asn Asn Glu Gly Arg Arg 
        35                  40                  45              


Ser Lys Arg Gly Ser Arg Arg Leu Lys Arg Arg Arg Ile His Arg Leu 
    50                  55                  60                  


Glu Arg Val Lys Leu Leu Leu Thr Glu Tyr Asp Leu Ile Asn Lys Glu 
65                  70                  75                  80  


Gln Ile Pro Thr Ser Asn Asn Pro Tyr Gln Ile Arg Val Lys Gly Leu 
                85                  90                  95      


Ser Glu Ile Leu Ser Lys Asp Glu Leu Ala Ile Ala Leu Leu His Leu 
            100                 105                 110         


Ala Lys Arg Arg Gly Ile His Asn Ile Asn Val Ser Ser Glu Asp Glu 
        115                 120                 125             


Asp Ala Ser Asn Glu Leu Ser Thr Lys Glu Gln Ile Asn Arg Asn Asn 
    130                 135                 140                 


Lys Leu Leu Lys Asp Lys Tyr Val Cys Glu Val Gln Leu Gln Arg Leu 
145                 150                 155                 160 


Lys Glu Gly Gln Ile Arg Gly Glu Lys Asn Arg Phe Lys Thr Thr Asp 
                165                 170                 175     


Ile Leu Lys Glu Ile Asp Gln Leu Leu Lys Val Gln Lys Asp Tyr His 
            180                 185                 190         


Asn Leu Asp Ile Asp Phe Ile Asn Gln Tyr Lys Glu Ile Val Glu Thr 
        195                 200                 205             


Arg Arg Glu Tyr Phe Glu Gly Pro Gly Gln Gly Ser Pro Phe Gly Trp 
    210                 215                 220                 


Asn Gly Asp Leu Lys Lys Trp Tyr Glu Met Leu Met Gly His Xaa Thr 
225                 230                 235                 240 


Tyr Phe Pro Gln Glu Leu Arg Ser Val Lys Tyr Ala Tyr Ser Ala Asp 
                245                 250                 255     


Leu Phe Asn Ala Leu Asn Asp Leu Asn Asn Leu Ile Ile Gln Arg Asp 
            260                 265                 270         


Asn Ser Glu Lys Leu Glu Tyr His Glu Lys Tyr His Ile Ile Glu Asn 
        275                 280                 285             


Val Phe Lys Gln Lys Lys Lys Pro Thr Leu Lys Gln Ile Ala Lys Glu 
    290                 295                 300                 


Ile Gly Val Asn Pro Glu Asp Ile Lys Gly Tyr Arg Ile Thr Lys Ser 
305                 310                 315                 320 


Gly Thr Pro Gln Phe Thr Glu Phe Lys Leu Tyr His Asp Leu Lys Ser 
                325                 330                 335     


Ile Val Phe Asp Lys Ser Ile Leu Glu Asn Glu Ala Ile Leu Asp Gln 
            340                 345                 350         


Ile Ala Glu Ile Leu Thr Ile Tyr Gln Asp Glu Gln Ser Ile Lys Glu 
        355                 360                 365             


Glu Leu Asn Lys Leu Pro Glu Ile Leu Asn Glu Gln Asp Lys Ala Glu 
    370                 375                 380                 


Ile Ala Lys Leu Ile Gly Tyr Asn Gly Thr His Arg Leu Ser Leu Lys 
385                 390                 395                 400 


Xaa Ile His Leu Ile Asn Glu Xaa Leu Trp Gln Thr Ser Xaa Asn Gln 
                405                 410                 415     


Met Xaa Ile Phe Asn Xaa Leu Asn Ile Lys Pro Asn Lys Val Asp Leu 
            420                 425                 430         


Ser Glu Gln Asn Lys Ile Pro Lys Asp Met Val Asn Asp Phe Ile Leu 
        435                 440                 445             


Ser Pro Val Val Lys Arg Thr Phe Ile Gln Ser Ile Asn Val Ile Asn 
    450                 455                 460                 


Lys Val Ile Glu Lys Tyr Gly Ile Pro Glu Asp Ile Ile Ile Glu Leu 
465                 470                 475                 480 


Ala Arg Glu Asn Asn Ser Asp Asp Arg Lys Lys Phe Ile Asn Asn Leu 
                485                 490                 495     


Gln Lys Lys Asn Glu Ala Thr Arg Lys Arg Ile Asn Glu Ile Ile Gly 
            500                 505                 510         


Gln Thr Gly Asn Gln Asn Ala Lys Arg Ile Val Glu Lys Ile Arg Leu 
        515                 520                 525             


His Asp Gln Gln Glu Gly Lys Cys Leu Tyr Ser Leu Glu Ser Ile Ala 
    530                 535                 540                 


Leu Met Asp Leu Leu Asn Asn Pro Gln Asn Tyr Glu Val Asp His Ile 
545                 550                 555                 560 


Ile Pro Arg Ser Val Ala Phe Asp Asn Ser Ile His Asn Lys Val Leu 
                565                 570                 575     


Val Lys Gln Ile Glu Asn Ser Lys Lys Gly Asn Arg Thr Pro Tyr Gln 
            580                 585                 590         


Tyr Leu Asn Ser Ser Asp Ala Lys Leu Ser Tyr Asn Gln Phe Lys Gln 
        595                 600                 605             


His Ile Leu Asn Leu Ser Lys Ser Lys Asp Arg Ile Ser Lys Lys Lys 
    610                 615                 620                 


Lys Asp Tyr Leu Leu Glu Glu Arg Asp Ile Asn Lys Phe Glu Val Gln 
625                 630                 635                 640 


Lys Glu Phe Ile Asn Arg Asn Leu Val Asp Thr Arg Tyr Ala Thr Arg 
                645                 650                 655     


Glu Leu Thr Ser Tyr Leu Lys Ala Tyr Phe Ser Ala Asn Asn Met Asp 
            660                 665                 670         


Val Lys Val Lys Thr Ile Asn Gly Ser Phe Thr Asn His Leu Arg Lys 
        675                 680                 685             


Val Trp Arg Phe Asp Lys Tyr Arg Asn His Gly Tyr Lys His His Ala 
    690                 695                 700                 


Glu Asp Ala Leu Ile Ile Ala Asn Ala Asp Phe Leu Phe Lys Glu Asn 
705                 710                 715                 720 


Lys Lys Leu Gln Asn Thr Asn Lys Ile Leu Glu Lys Pro Thr Ile Glu 
                725                 730                 735     


Asn Asn Thr Lys Lys Val Thr Val Glu Lys Glu Glu Asp Tyr Asn Asn 
            740                 745                 750         


Val Phe Glu Thr Pro Lys Leu Val Glu Asp Ile Lys Gln Tyr Arg Asp 
        755                 760                 765             


Tyr Lys Phe Ser His Arg Val Asp Lys Lys Pro Asn Arg Gln Leu Ile 
    770                 775                 780                 


Asn Asp Thr Leu Tyr Ser Thr Arg Met Lys Asp Glu His Asp Tyr Ile 
785                 790                 795                 800 


Val Gln Thr Ile Thr Asp Ile Tyr Gly Lys Asp Asn Thr Asn Leu Lys 
                805                 810                 815     


Lys Gln Phe Asn Lys Asn Pro Glu Lys Phe Leu Met Tyr Gln Asn Asp 
            820                 825                 830         


Pro Lys Thr Phe Glu Lys Leu Ser Ile Ile Met Lys Gln Tyr Ser Asp 
        835                 840                 845             


Glu Lys Asn Pro Leu Ala Lys Tyr Tyr Glu Glu Thr Gly Glu Tyr Leu 
    850                 855                 860                 


Thr Lys Tyr Ser Lys Lys Asn Asn Gly Pro Ile Val Lys Lys Ile Lys 
865                 870                 875                 880 


Leu Leu Gly Asn Lys Val Gly Asn His Leu Asp Val Thr Asn Lys Tyr 
                885                 890                 895     


Glu Asn Ser Thr Lys Lys Leu Val Lys Leu Ser Ile Lys Asn Tyr Arg 
            900                 905                 910         


Phe Asp Val Tyr Leu Thr Glu Lys Gly Tyr Lys Phe Val Thr Ile Ala 
        915                 920                 925             


Tyr Leu Asn Val Phe Lys Lys Asp Asn Tyr Tyr Tyr Ile Pro Lys Asp 
    930                 935                 940                 


Lys Tyr Gln Glu Leu Lys Glu Lys Lys Lys Ile Lys Asp Thr Asp Gln 
945                 950                 955                 960 


Phe Ile Ala Ser Phe Tyr Lys Asn Asp Leu Ile Lys Leu Asn Gly Asp 
                965                 970                 975     


Leu Tyr Lys Ile Ile Gly Val Asn Ser Asp Asp Arg Asn Ile Ile Glu 
            980                 985                 990         


Leu Asp Tyr Tyr Asp Ile Lys Tyr  Lys Asp Tyr Cys Glu  Ile Asn Asn 
        995                 1000                 1005             


Ile Lys  Gly Glu Pro Arg Ile  Lys Lys Thr Ile Gly  Lys Lys Thr 
    1010                 1015                 1020             


Glu Ser  Ile Glu Lys Phe Thr  Thr Asp Val Leu Gly  Asn Leu Tyr 
    1025                 1030                 1035             


Leu His  Ser Thr Glu Lys Ala  Pro Gln Leu Ile Phe  Lys Arg Gly 
    1040                 1045                 1050             


Leu 
    


<210>  4
<211>  1051
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<223>  The sequence CANNOT be where X equals the following combination -
       408: D, 414: L, 418: E, 422: K,  239: C, and 401: C

<220>
<221>  misc_feature
<222>  (1)..(1051)
<223>  M-ShyCas9_X

<220>
<221>  misc_feature
<222>  (239)..(239)
<223>  X can be any of these amino acids:  C, S, A

<220>
<221>  misc_feature
<222>  (401)..(401)
<223>  X can be any of these amino acids:  C,  S, A

<220>
<221>  misc_feature
<222>  (408)..(408)
<223>  X can be any of these amino acids:  D, G,S,T,A,E

<220>
<221>  misc_feature
<222>  (414)..(414)
<223>  X can be any of these amino acids: L, G,S,T,A, D, E

<220>
<221>  misc_feature
<222>  (418)..(418)
<223>  X can be any of these amino acids: E, G, S, T, A, D

<220>
<221>  misc_feature
<222>  (422)..(422)
<223>  X can be any of these amino acids: K,  A, G, S, T, D, E

<400>  4

Met Asn Asn Tyr Ile Leu Gly Leu Asp Ile Gly Ile Thr Ser Val Gly 
1               5                   10                  15      


Tyr Gly Ile Val Asp Ser Asp Thr Arg Glu Ile Lys Asp Ala Gly Val 
            20                  25                  30          


Arg Leu Phe Pro Glu Ala Asn Val Asp Asn Asn Glu Gly Arg Arg Ser 
        35                  40                  45              


Lys Arg Gly Ala Arg Arg Leu Lys Arg Arg Arg Ile His Arg Leu Asp 
    50                  55                  60                  


Arg Val Lys His Leu Leu Ala Glu Tyr Asp Leu Leu Asp Leu Thr Asn 
65                  70                  75                  80  


Ile Pro Lys Ser Thr Asn Pro Tyr Gln Thr Arg Val Lys Gly Leu Asn 
                85                  90                  95      


Glu Lys Leu Ser Lys Asp Glu Leu Val Ile Ala Leu Leu His Ile Ala 
            100                 105                 110         


Lys Arg Arg Gly Ile His Asn Val Asn Val Met Met Asp Asp Asn Asp 
        115                 120                 125             


Ser Gly Asn Glu Leu Ser Thr Lys Asp Gln Leu Lys Lys Asn Ala Lys 
    130                 135                 140                 


Ala Leu Ser Asp Lys Tyr Val Cys Glu Leu Gln Leu Glu Arg Phe Glu 
145                 150                 155                 160 


Gln Asp Tyr Lys Val Arg Gly Glu Lys Asn Arg Phe Lys Thr Glu Asp 
                165                 170                 175     


Phe Val Arg Glu Ala Arg Lys Leu Leu Glu Thr Gln Ser Lys Phe Phe 
            180                 185                 190         


Glu Ile Asp Gln Thr Phe Ile Met Arg Tyr Ile Glu Leu Ile Glu Thr 
        195                 200                 205             


Arg Arg Glu Tyr Phe Glu Gly Pro Gly Lys Gly Ser Pro Phe Gly Trp 
    210                 215                 220                 


Glu Gly Asn Ile Lys Lys Trp Phe Glu Gln Met Met Gly His Xaa Thr 
225                 230                 235                 240 


Tyr Phe Pro Glu Glu Leu Arg Ser Val Lys Tyr Ser Tyr Ser Ala Glu 
                245                 250                 255     


Leu Phe Asn Ala Leu Asn Asp Leu Asn Asn Leu Val Ile Thr Arg Asp 
            260                 265                 270         


Glu Asp Ala Lys Leu Asn Tyr Gly Glu Lys Phe Gln Ile Ile Glu Asn 
        275                 280                 285             


Val Phe Lys Gln Lys Lys Thr Pro Asn Leu Lys Gln Ile Ala Ile Glu 
    290                 295                 300                 


Ile Gly Val His Glu Thr Glu Ile Lys Gly Tyr Arg Val Asn Lys Ser 
305                 310                 315                 320 


Gly Lys Pro Glu Phe Thr Gln Phe Lys Leu Tyr His Asp Leu Lys Asn 
                325                 330                 335     


Ile Phe Lys Asp Pro Lys Tyr Leu Asn Asp Ile Gln Leu Met Asp Asn 
            340                 345                 350         


Ile Ala Glu Ile Ile Thr Ile Tyr Gln Asp Ala Glu Ser Ile Ile Lys 
        355                 360                 365             


Glu Leu Asn Gln Leu Pro Glu Leu Leu Ser Glu Arg Glu Lys Glu Lys 
    370                 375                 380                 


Ile Ser Ala Leu Ser Gly Tyr Ser Gly Thr His Arg Leu Ser Leu Lys 
385                 390                 395                 400 


Xaa Ile Asn Leu Leu Leu Asp Xaa Leu Trp Glu Ser Ser Xaa Asn Gln 
                405                 410                 415     


Met Xaa Leu Phe Thr Xaa Leu Asn Leu Lys Pro Lys Lys Ile Asp Leu 
            420                 425                 430         


Ser Gln Gln His Lys Ile Pro Ser Lys Leu Val Asp Asp Phe Ile Leu 
        435                 440                 445             


Ser Pro Val Val Lys Arg Ala Phe Ile Gln Ser Ile Gln Val Val Asn 
    450                 455                 460                 


Ala Ile Ile Asp Lys Tyr Gly Leu Pro Glu Asp Ile Ile Ile Glu Leu 
465                 470                 475                 480 


Ala Arg Glu Asn Asn Ser Asp Asp Arg Arg Lys Phe Leu Asn Gln Leu 
                485                 490                 495     


Gln Lys Gln Asn Glu Glu Thr Arg Lys Gln Val Glu Lys Val Leu Arg 
            500                 505                 510         


Glu Tyr Gly Asn Asp Asn Ala Lys Arg Ile Val Gln Lys Ile Lys Leu 
        515                 520                 525             


His Asn Met Gln Glu Gly Lys Cys Leu Tyr Ser Leu Lys Asp Ile Pro 
    530                 535                 540                 


Leu Glu Asp Leu Leu Arg Asn Pro His His Tyr Glu Val Asp His Ile 
545                 550                 555                 560 


Ile Pro Arg Ser Val Ala Phe Asp Asn Ser Met His Asn Lys Val Leu 
                565                 570                 575     


Val Arg Ala Asp Glu Asn Ser Lys Lys Gly Asn Arg Thr Pro Tyr Gln 
            580                 585                 590         


Tyr Leu Asn Ser Ser Glu Ser Ser Leu Ser Tyr Asn Glu Phe Lys Gln 
        595                 600                 605             


His Ile Leu Asn Leu Ser Lys Thr Lys Asp Arg Ile Thr Lys Lys Lys 
    610                 615                 620                 


Arg Glu Tyr Leu Leu Glu Glu Arg Asp Ile Asn Lys Phe Asp Val Gln 
625                 630                 635                 640 


Lys Glu Phe Ile Asn Arg Asn Leu Val Asp Thr Arg Tyr Ala Thr Arg 
                645                 650                 655     


Glu Leu Thr Ser Leu Leu Lys Ala Tyr Phe Ser Ala Asn Asn Leu Asp 
            660                 665                 670         


Val Lys Val Lys Thr Ile Asn Gly Ser Phe Thr Asn Tyr Leu Arg Lys 
        675                 680                 685             


Val Trp Lys Phe Asp Lys Asp Arg Asn Lys Gly Tyr Lys His His Ala 
    690                 695                 700                 


Glu Asp Ala Leu Ile Ile Ala Asn Ala Asp Phe Leu Phe Lys His Asn 
705                 710                 715                 720 


Lys Lys Leu Arg Asn Ile Asn Lys Val Leu Asp Ala Pro Ser Lys Glu 
                725                 730                 735     


Val Asp Lys Lys Arg Val Thr Val Gln Ser Glu Asp Glu Tyr Asn Gln 
            740                 745                 750         


Ile Phe Glu Asp Thr Gln Lys Ala Gln Ala Ile Lys Lys Phe Glu Ile 
        755                 760                 765             


Arg Lys Phe Ser His Arg Val Asp Lys Lys Pro Asn Arg Gln Leu Ile 
    770                 775                 780                 


Asn Asp Thr Leu Tyr Ser Thr Arg Asn Ile Asp Gly Ile Glu Tyr Val 
785                 790                 795                 800 


Val Glu Ser Ile Lys Asp Ile Tyr Ser Val Asn Asn Asp Lys Val Lys 
                805                 810                 815     


Thr Lys Phe Lys Lys Asp Pro His Arg Leu Leu Met Tyr Arg Asn Asp 
            820                 825                 830         


Pro Gln Thr Phe Glu Lys Phe Glu Lys Val Phe Lys Gln Tyr Glu Ser 
        835                 840                 845             


Glu Lys Asn Pro Phe Ala Lys Tyr Tyr Glu Glu Thr Gly Glu Lys Ile 
    850                 855                 860                 


Arg Lys Phe Ser Lys Thr Gly Gln Gly Pro Tyr Ile Asn Lys Ile Lys 
865                 870                 875                 880 


Tyr Leu Arg Glu Arg Leu Gly Arg His Cys Asp Val Thr Asn Lys Tyr 
                885                 890                 895     


Ile Asn Ser Arg Asn Lys Ile Val Gln Leu Lys Ile Tyr Ser Tyr Arg 
            900                 905                 910         


Phe Asp Ile Tyr Gln Tyr Gly Asn Asn Tyr Lys Met Ile Thr Ile Ser 
        915                 920                 925             


Tyr Ile Asp Leu Glu Gln Lys Ser Asn Tyr Tyr Tyr Ile Ser Arg Glu 
    930                 935                 940                 


Lys Tyr Glu Gln Lys Lys Lys Asp Lys Gln Ile Asp Asp Ser Tyr Lys 
945                 950                 955                 960 


Phe Ile Gly Ser Phe Tyr Lys Asn Asp Ile Ile Asn Tyr Asn Gly Glu 
                965                 970                 975     


Met Tyr Arg Val Ile Gly Val Asn Asp Ser Glu Lys Asn Lys Ile Gln 
            980                 985                 990         


Leu Asp Met Ile Asp Ile Ser Ile  Lys Asp Tyr Met Glu  Leu Asn Asn 
        995                 1000                 1005             


Ile Lys  Lys Thr Gly Val Ile  Tyr Lys Thr Ile Gly  Lys Ser Thr 
    1010                 1015                 1020             


Thr His  Ile Glu Lys Tyr Thr  Thr Asp Ile Leu Gly  Asn Leu Tyr 
    1025                 1030                 1035             


Lys Ala  Ala Pro Pro Lys Lys  Pro Gln Leu Ile Phe  Lys 
    1040                 1045                 1050     


<210>  5
<211>  1052
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<223>  The sequence CANNOT be where X equals the following combination -
       410: E, 416: M, 420: E, 424: Y, 241: C, and 403: C

<220>
<221>  misc_feature
<222>  (1)..(1052)
<223>  M-SmiCas9_X

<220>
<221>  misc_feature
<222>  (241)..(241)
<223>  X can be any of these amino acids: C, S, A

<220>
<221>  misc_feature
<222>  (403)..(403)
<223>  X can be any of these amino acids:  C, S, A

<220>
<221>  misc_feature
<222>  (410)..(410)
<223>  X can be any of these amino acids: E,  G, S, T, A, D

<220>
<221>  misc_feature
<222>  (416)..(416)
<223>  X can be any of these amino acids: M, G,S,T,A, D, E, L

<220>
<221>  misc_feature
<222>  (420)..(420)
<223>  X can be any of these amino acids: E, G, S, T, A, D

<220>
<221>  misc_feature
<222>  (424)..(424)
<223>  X can be any of these amino acids: Y,  A, G, S, T, D, E

<400>  5

Met Glu Lys Asp Tyr Ile Leu Gly Leu Asp Ile Gly Ile Gly Ser Val 
1               5                   10                  15      


Gly Tyr Gly Leu Ile Asp Tyr Asp Thr Lys Ser Ile Ile Asp Ala Gly 
            20                  25                  30          


Val Arg Leu Phe Pro Glu Ala Asn Ala Asp Asn Asn Leu Gly Arg Arg 
        35                  40                  45              


Ala Lys Arg Gly Ala Arg Arg Leu Lys Arg Arg Arg Ile His Arg Leu 
    50                  55                  60                  


Glu Arg Val Lys Ser Leu Leu Ser Glu Tyr Lys Ile Ile Ser Gly Leu 
65                  70                  75                  80  


Ala Pro Thr Asn Asn Gln Pro Tyr Asn Ile Arg Val Lys Gly Leu Thr 
                85                  90                  95      


Glu Gln Leu Thr Lys Asp Glu Leu Ala Val Ala Leu Leu His Ile Ala 
            100                 105                 110         


Lys Arg Arg Gly Ile His Asn Val Asp Val Ala Ala Asp Lys Glu Glu 
        115                 120                 125             


Thr Ala Ser Asp Ser Leu Ser Thr Lys Asp Gln Ile Asn Lys Asn Ala 
    130                 135                 140                 


Lys Phe Leu Glu Ser Arg Tyr Val Cys Glu Leu Gln Lys Glu Arg Leu 
145                 150                 155                 160 


Glu Asn Glu Gly His Val Arg Gly Val Glu Asn Arg Phe Leu Thr Lys 
                165                 170                 175     


Asp Ile Val Arg Glu Ala Lys Lys Ile Ile Asp Thr Gln Met Gln Tyr 
            180                 185                 190         


Tyr Pro Glu Ile Asp Glu Thr Phe Lys Glu Lys Tyr Ile Ser Leu Val 
        195                 200                 205             


Glu Thr Arg Arg Glu Tyr Tyr Glu Gly Pro Gly Lys Gly Ser Pro Tyr 
    210                 215                 220                 


Gly Trp Asp Ala Asp Val Lys Lys Trp Tyr Gln Leu Met Met Gly His 
225                 230                 235                 240 


Xaa Thr Tyr Phe Pro Val Glu Phe Arg Ser Val Lys Tyr Ala Tyr Thr 
                245                 250                 255     


Ala Asp Leu Tyr Asn Ala Leu Asn Asp Leu Asn Asn Leu Thr Ile Ala 
            260                 265                 270         


Arg Asp Asp Asn Pro Lys Leu Glu Tyr His Glu Lys Tyr His Ile Ile 
        275                 280                 285             


Glu Asn Val Phe Lys Gln Lys Arg Asn Pro Thr Leu Lys Gln Ile Ala 
    290                 295                 300                 


Lys Glu Ile Gly Val Asn Asp Ile Asn Ile Ser Gly Tyr Arg Val Thr 
305                 310                 315                 320 


Lys Ser Gly Lys Pro Gln Phe Thr Ser Phe Lys Leu Phe His Asp Leu 
                325                 330                 335     


Lys Lys Val Val Lys Asp His Ala Ile Leu Asp Asp Ile Asp Leu Leu 
            340                 345                 350         


Asn Gln Ile Ala Glu Ile Leu Thr Ile Tyr Gln Asp Lys Asp Ser Ile 
        355                 360                 365             


Val Ala Glu Leu Gly Gln Leu Glu Tyr Leu Met Ser Glu Ala Asp Lys 
    370                 375                 380                 


Gln Ser Ile Ser Glu Leu Thr Gly Tyr Thr Gly Thr His Ser Leu Ser 
385                 390                 395                 400 


Leu Lys Xaa Met Asn Met Ile Ile Asp Xaa Leu Trp His Ser Ser Xaa 
                405                 410                 415     


Asn Gln Met Xaa Val Phe Thr Xaa Leu Asn Met Arg Pro Lys Lys Tyr 
            420                 425                 430         


Glu Leu Lys Gly Tyr Gln Arg Ile Pro Thr Asp Met Ile Asp Asp Ala 
        435                 440                 445             


Ile Leu Ser Pro Val Val Lys Arg Ser Phe Lys Gln Ala Ile Gly Val 
    450                 455                 460                 


Val Asn Ala Ile Ile Lys Lys Tyr Gly Leu Pro Lys Asp Ile Ile Ile 
465                 470                 475                 480 


Glu Leu Ala Arg Glu Ser Asn Ser Ala Glu Lys Ser Arg Tyr Leu Arg 
                485                 490                 495     


Ala Ile Gln Lys Lys Asn Glu Lys Thr Arg Glu Arg Ile Glu Ala Ile 
            500                 505                 510         


Ile Lys Glu Tyr Gly Asn Glu Asn Ala Lys Gly Leu Val Gln Lys Ile 
        515                 520                 525             


Lys Leu His Asp Ala Gln Glu Gly Lys Cys Leu Tyr Ser Leu Lys Asp 
    530                 535                 540                 


Ile Pro Leu Glu Asp Leu Leu Arg Asn Pro Asn Asn Tyr Asp Ile Asp 
545                 550                 555                 560 


His Ile Ile Pro Arg Ser Val Ser Phe Asp Asp Ser Met His Asn Lys 
                565                 570                 575     


Val Leu Val Arg Arg Glu Gln Asn Ala Lys Lys Asn Asn Gln Thr Pro 
            580                 585                 590         


Tyr Gln Tyr Leu Thr Ser Gly Tyr Ala Asp Ile Lys Tyr Ser Val Phe 
        595                 600                 605             


Lys Gln His Val Leu Asn Leu Ala Glu Asn Lys Asp Arg Met Thr Lys 
    610                 615                 620                 


Lys Lys Arg Glu Tyr Leu Leu Glu Glu Arg Asn Ile Asn Lys Tyr Asp 
625                 630                 635                 640 


Val Gln Lys Glu Phe Ile Asn Arg Asn Leu Val Asp Thr Arg Tyr Thr 
                645                 650                 655     


Thr Arg Glu Leu Thr Thr Leu Leu Lys Thr Tyr Phe Thr Ile Asn Asn 
            660                 665                 670         


Leu Asp Val Lys Val Lys Thr Ile Asn Gly Ser Phe Thr Asp Phe Leu 
        675                 680                 685             


Arg Lys Arg Trp Gly Phe Lys Lys Asn Arg Asp Glu Gly Tyr Lys His 
    690                 695                 700                 


His Ala Glu Asp Ala Leu Ile Ile Ala Asn Ala Asp Tyr Leu Phe Lys 
705                 710                 715                 720 


Glu His Lys Leu Leu Lys Glu Ile Lys Asp Val Ser Asp Leu Ala Gly 
                725                 730                 735     


Asp Glu Arg Asn Ser Asn Val Lys Asp Glu Asp Gln Tyr Glu Glu Val 
            740                 745                 750         


Phe Gly Gly Tyr Phe Lys Ile Glu Asp Ile Lys Lys Tyr Lys Ile Lys 
        755                 760                 765             


Lys Phe Ser His Arg Val Asp Lys Lys Pro Asn Arg Gln Leu Ile Asn 
    770                 775                 780                 


Asp Thr Ile Tyr Ser Thr Arg Val Lys Asp Asp Lys Arg Tyr Leu Ile 
785                 790                 795                 800 


Asn Thr Leu Lys Asn Leu Tyr Asp Lys Ser Asn Gly Asp Leu Lys Glu 
                805                 810                 815     


Arg Met Gln Lys Asp Pro Glu Ser Leu Leu Met Tyr His His Asp Pro 
            820                 825                 830         


Gln Thr Phe Glu Lys Leu Lys Ile Val Met Ser Gln Tyr Glu Asn Glu 
        835                 840                 845             


Lys Asn Pro Leu Ala Lys Tyr Phe Glu Glu Thr Gly Gln Tyr Leu Thr 
    850                 855                 860                 


Lys Tyr Ala Lys His Asp Asn Gly Pro Ala Ile His Lys Ile Lys Tyr 
865                 870                 875                 880 


Tyr Gly Asn Lys Leu Val Glu His Leu Asp Ile Thr Lys Asn Tyr His 
                885                 890                 895     


Asn Pro Gln Asn Lys Val Val Gln Leu Ser Gln Lys Ser Phe Arg Phe 
            900                 905                 910         


Asp Val Tyr Gln Thr Asp Lys Gly Tyr Lys Phe Ile Ser Ile Ala Tyr 
        915                 920                 925             


Leu Thr Leu Lys Asn Glu Lys Asn Tyr Tyr Ala Ile Ser Gln Glu Lys 
    930                 935                 940                 


Tyr Asp Gln Leu Lys Ser Glu Lys Lys Ile Ser Asn Asn Ala Val Phe 
945                 950                 955                 960 


Ile Gly Ser Phe Tyr Thr Ser Asp Ile Ile Glu Ile Asn Asn Glu Lys 
                965                 970                 975     


Phe Arg Val Ile Gly Val Asn Ser Asp Lys Asn Asn Leu Ile Glu Val 
            980                 985                 990         


Asp Arg Ile Asp Ile Arg Gln Lys  Glu Phe Ile Glu Leu  Glu Glu Glu 
        995                 1000                 1005             


Lys Lys  Asn Asn Arg Ile Lys  Val Thr Ile Gly Arg  Lys Thr Thr 
    1010                 1015                 1020             


Asn Ile  Glu Lys Phe His Thr  Asp Ile Leu Gly Asn  Met Tyr Lys 
    1025                 1030                 1035             


Ser Lys  Arg Pro Lys Ala Pro  Gln Leu Val Phe Lys  Lys Gly 
    1040                 1045                 1050         


<210>  6
<211>  1053
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(1053)
<223>  M-SauCas9R420A

<400>  6

Met Lys Arg Asn Tyr Ile Leu Gly Leu Asp Ile Gly Ile Thr Ser Val 
1               5                   10                  15      


Gly Tyr Gly Ile Ile Asp Tyr Glu Thr Arg Asp Val Ile Asp Ala Gly 
            20                  25                  30          


Val Arg Leu Phe Lys Glu Ala Asn Val Glu Asn Asn Glu Gly Arg Arg 
        35                  40                  45              


Ser Lys Arg Gly Ala Arg Arg Leu Lys Arg Arg Arg Arg His Arg Ile 
    50                  55                  60                  


Gln Arg Val Lys Lys Leu Leu Phe Asp Tyr Asn Leu Leu Thr Asp His 
65                  70                  75                  80  


Ser Glu Leu Ser Gly Ile Asn Pro Tyr Glu Ala Arg Val Lys Gly Leu 
                85                  90                  95      


Ser Gln Lys Leu Ser Glu Glu Glu Phe Ser Ala Ala Leu Leu His Leu 
            100                 105                 110         


Ala Lys Arg Arg Gly Val His Asn Val Asn Glu Val Glu Glu Asp Thr 
        115                 120                 125             


Gly Asn Glu Leu Ser Thr Lys Glu Gln Ile Ser Arg Asn Ser Lys Ala 
    130                 135                 140                 


Leu Glu Glu Lys Tyr Val Ala Glu Leu Gln Leu Glu Arg Leu Lys Lys 
145                 150                 155                 160 


Asp Gly Glu Val Arg Gly Ser Ile Asn Arg Phe Lys Thr Ser Asp Tyr 
                165                 170                 175     


Val Lys Glu Ala Lys Gln Leu Leu Lys Val Gln Lys Ala Tyr His Gln 
            180                 185                 190         


Leu Asp Gln Ser Phe Ile Asp Thr Tyr Ile Asp Leu Leu Glu Thr Arg 
        195                 200                 205             


Arg Thr Tyr Tyr Glu Gly Pro Gly Glu Gly Ser Pro Phe Gly Trp Lys 
    210                 215                 220                 


Asp Ile Lys Glu Trp Tyr Glu Met Leu Met Gly His Cys Thr Tyr Phe 
225                 230                 235                 240 


Pro Glu Glu Leu Arg Ser Val Lys Tyr Ala Tyr Asn Ala Asp Leu Tyr 
                245                 250                 255     


Asn Ala Leu Asn Asp Leu Asn Asn Leu Val Ile Thr Arg Asp Glu Asn 
            260                 265                 270         


Glu Lys Leu Glu Tyr Tyr Glu Lys Phe Gln Ile Ile Glu Asn Val Phe 
        275                 280                 285             


Lys Gln Lys Lys Lys Pro Thr Leu Lys Gln Ile Ala Lys Glu Ile Leu 
    290                 295                 300                 


Val Asn Glu Glu Asp Ile Lys Gly Tyr Arg Val Thr Ser Thr Gly Lys 
305                 310                 315                 320 


Pro Glu Phe Thr Asn Leu Lys Val Tyr His Asp Ile Lys Asp Ile Thr 
                325                 330                 335     


Ala Arg Lys Glu Ile Ile Glu Asn Ala Glu Leu Leu Asp Gln Ile Ala 
            340                 345                 350         


Lys Ile Leu Thr Ile Tyr Gln Ser Ser Glu Asp Ile Gln Glu Glu Leu 
        355                 360                 365             


Thr Asn Leu Asn Ser Glu Leu Thr Gln Glu Glu Ile Glu Gln Ile Ser 
    370                 375                 380                 


Asn Leu Lys Gly Tyr Thr Gly Thr His Asn Leu Ser Leu Lys Ala Ile 
385                 390                 395                 400 


Asn Leu Ile Leu Asp Glu Leu Trp His Thr Asn Asp Asn Gln Ile Ala 
                405                 410                 415     


Ile Phe Asn Ala Leu Lys Leu Val Pro Lys Lys Val Asp Leu Ser Gln 
            420                 425                 430         


Gln Lys Glu Ile Pro Thr Thr Leu Val Asp Asp Phe Ile Leu Ser Pro 
        435                 440                 445             


Val Val Lys Arg Ser Phe Ile Gln Ser Ile Lys Val Ile Asn Ala Ile 
    450                 455                 460                 


Ile Lys Lys Tyr Gly Leu Pro Asn Asp Ile Ile Ile Glu Leu Ala Arg 
465                 470                 475                 480 


Glu Lys Asn Ser Lys Asp Ala Gln Lys Met Ile Asn Glu Met Gln Lys 
                485                 490                 495     


Arg Asn Arg Gln Thr Asn Glu Arg Ile Glu Glu Ile Ile Arg Thr Thr 
            500                 505                 510         


Gly Lys Glu Asn Ala Lys Tyr Leu Ile Glu Lys Ile Lys Leu His Asp 
        515                 520                 525             


Met Gln Glu Gly Lys Cys Leu Tyr Ser Leu Glu Ala Ile Pro Leu Glu 
    530                 535                 540                 


Asp Leu Leu Asn Asn Pro Phe Asn Tyr Glu Val Asp His Ile Ile Pro 
545                 550                 555                 560 


Arg Ser Val Ser Phe Asp Asn Ser Phe Asn Asn Lys Val Leu Val Lys 
                565                 570                 575     


Gln Glu Glu Asn Ser Lys Lys Gly Asn Arg Thr Pro Phe Gln Tyr Leu 
            580                 585                 590         


Ser Ser Ser Asp Ser Lys Ile Ser Tyr Glu Thr Phe Lys Lys His Ile 
        595                 600                 605             


Leu Asn Leu Ala Lys Gly Lys Gly Arg Ile Ser Lys Thr Lys Lys Glu 
    610                 615                 620                 


Tyr Leu Leu Glu Glu Arg Asp Ile Asn Arg Phe Ser Val Gln Lys Asp 
625                 630                 635                 640 


Phe Ile Asn Arg Asn Leu Val Asp Thr Arg Tyr Ala Thr Arg Gly Leu 
                645                 650                 655     


Met Asn Leu Leu Arg Ser Tyr Phe Arg Val Asn Asn Leu Asp Val Lys 
            660                 665                 670         


Val Lys Ser Ile Asn Gly Gly Phe Thr Ser Phe Leu Arg Arg Lys Trp 
        675                 680                 685             


Lys Phe Lys Lys Glu Arg Asn Lys Gly Tyr Lys His His Ala Glu Asp 
    690                 695                 700                 


Ala Leu Ile Ile Ala Asn Ala Asp Phe Ile Phe Lys Glu Trp Lys Lys 
705                 710                 715                 720 


Leu Asp Lys Ala Lys Lys Val Met Glu Asn Gln Met Phe Glu Glu Lys 
                725                 730                 735     


Gln Ala Glu Ser Met Pro Glu Ile Glu Thr Glu Gln Glu Tyr Lys Glu 
            740                 745                 750         


Ile Phe Ile Thr Pro His Gln Ile Lys His Ile Lys Asp Phe Lys Asp 
        755                 760                 765             


Tyr Lys Tyr Ser His Arg Val Asp Lys Lys Pro Asn Arg Glu Leu Ile 
    770                 775                 780                 


Asn Asp Thr Leu Tyr Ser Thr Arg Lys Asp Asp Lys Gly Asn Thr Leu 
785                 790                 795                 800 


Ile Val Asn Asn Leu Asn Gly Leu Tyr Asp Lys Asp Asn Asp Lys Leu 
                805                 810                 815     


Lys Lys Leu Ile Asn Lys Ser Pro Glu Lys Leu Leu Met Tyr His His 
            820                 825                 830         


Asp Pro Gln Thr Tyr Gln Lys Leu Lys Leu Ile Met Glu Gln Tyr Gly 
        835                 840                 845             


Asp Glu Lys Asn Pro Leu Tyr Lys Tyr Tyr Glu Glu Thr Gly Asn Tyr 
    850                 855                 860                 


Leu Thr Lys Tyr Ser Lys Lys Asp Asn Gly Pro Val Ile Lys Lys Ile 
865                 870                 875                 880 


Lys Tyr Tyr Gly Asn Lys Leu Asn Ala His Leu Asp Ile Thr Asp Asp 
                885                 890                 895     


Tyr Pro Asn Ser Arg Asn Lys Val Val Lys Leu Ser Leu Lys Pro Tyr 
            900                 905                 910         


Arg Phe Asp Val Tyr Leu Asp Asn Gly Val Tyr Lys Phe Val Thr Val 
        915                 920                 925             


Lys Asn Leu Asp Val Ile Lys Lys Glu Asn Tyr Tyr Glu Val Asn Ser 
    930                 935                 940                 


Lys Cys Tyr Glu Glu Ala Lys Lys Leu Lys Lys Ile Ser Asn Gln Ala 
945                 950                 955                 960 


Glu Phe Ile Ala Ser Phe Tyr Asn Asn Asp Leu Ile Lys Ile Asn Gly 
                965                 970                 975     


Glu Leu Tyr Arg Val Ile Gly Val Asn Asn Asp Leu Leu Asn Arg Ile 
            980                 985                 990         


Glu Val Asn Met Ile Asp Ile Thr  Tyr Arg Glu Tyr Leu  Glu Asn Met 
        995                 1000                 1005             


Asn Asp  Lys Arg Pro Pro Arg  Ile Ile Lys Thr Ile  Ala Ser Lys 
    1010                 1015                 1020             


Thr Gln  Ser Ile Lys Lys Tyr  Ser Thr Asp Ile Leu  Gly Asn Leu 
    1025                 1030                 1035             


Tyr Glu  Val Lys Ser Lys Lys  His Pro Gln Ile Ile  Lys Lys Gly 
    1040                 1045                 1050             


<210>  7
<211>  1056
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(1056)
<223>  M-SluCas9R414A

<400>  7

Met Asn Gln Lys Phe Ile Leu Gly Leu Asp Ile Gly Ile Thr Ser Val 
1               5                   10                  15      


Gly Tyr Gly Leu Ile Asp Tyr Glu Thr Lys Asn Ile Ile Asp Ala Gly 
            20                  25                  30          


Val Arg Leu Phe Pro Glu Ala Asn Val Glu Asn Asn Glu Gly Arg Arg 
        35                  40                  45              


Ser Lys Arg Gly Ser Arg Arg Leu Lys Arg Arg Arg Ile His Arg Leu 
    50                  55                  60                  


Glu Arg Val Lys Lys Leu Leu Glu Asp Tyr Asn Leu Leu Asp Gln Ser 
65                  70                  75                  80  


Gln Ile Pro Gln Ser Thr Asn Pro Tyr Ala Ile Arg Val Lys Gly Leu 
                85                  90                  95      


Ser Glu Ala Leu Ser Lys Asp Glu Leu Val Ile Ala Leu Leu His Ile 
            100                 105                 110         


Ala Lys Arg Arg Gly Ile His Lys Ile Asp Val Ile Asp Ser Asn Asp 
        115                 120                 125             


Asp Val Gly Asn Glu Leu Ser Thr Lys Glu Gln Leu Asn Lys Asn Ser 
    130                 135                 140                 


Lys Leu Leu Lys Asp Lys Phe Val Cys Gln Ile Gln Leu Glu Arg Met 
145                 150                 155                 160 


Asn Glu Gly Gln Val Arg Gly Glu Lys Asn Arg Phe Lys Thr Ala Asp 
                165                 170                 175     


Ile Ile Lys Glu Ile Ile Gln Leu Leu Asn Val Gln Lys Asn Phe His 
            180                 185                 190         


Gln Leu Asp Glu Asn Phe Ile Asn Lys Tyr Ile Glu Leu Val Glu Met 
        195                 200                 205             


Arg Arg Glu Tyr Phe Glu Gly Pro Gly Lys Gly Ser Pro Tyr Gly Trp 
    210                 215                 220                 


Glu Gly Asp Pro Lys Ala Trp Tyr Glu Thr Leu Met Gly His Cys Thr 
225                 230                 235                 240 


Tyr Phe Pro Asp Glu Leu Arg Ser Val Lys Tyr Ala Tyr Ser Ala Asp 
                245                 250                 255     


Leu Phe Asn Ala Leu Asn Asp Leu Asn Asn Leu Val Ile Gln Arg Asp 
            260                 265                 270         


Gly Leu Ser Lys Leu Glu Tyr His Glu Lys Tyr His Ile Ile Glu Asn 
        275                 280                 285             


Val Phe Lys Gln Lys Lys Lys Pro Thr Leu Lys Gln Ile Ala Asn Glu 
    290                 295                 300                 


Ile Asn Val Asn Pro Glu Asp Ile Lys Gly Tyr Arg Ile Thr Lys Ser 
305                 310                 315                 320 


Gly Lys Pro Gln Phe Thr Glu Phe Lys Leu Tyr His Asp Leu Lys Ser 
                325                 330                 335     


Val Leu Phe Asp Gln Ser Ile Leu Glu Asn Glu Asp Val Leu Asp Gln 
            340                 345                 350         


Ile Ala Glu Ile Leu Thr Ile Tyr Gln Asp Lys Asp Ser Ile Lys Ser 
        355                 360                 365             


Lys Leu Thr Glu Leu Asp Ile Leu Leu Asn Glu Glu Asp Lys Glu Asn 
    370                 375                 380                 


Ile Ala Gln Leu Thr Gly Tyr Thr Gly Thr His Arg Leu Ser Leu Lys 
385                 390                 395                 400 


Cys Ile Arg Leu Val Leu Glu Glu Gln Trp Tyr Ser Ser Ala Asn Gln 
                405                 410                 415     


Met Glu Ile Phe Thr His Leu Asn Ile Lys Pro Lys Lys Ile Asn Leu 
            420                 425                 430         


Thr Ala Ala Asn Lys Ile Pro Lys Ala Met Ile Asp Glu Phe Ile Leu 
        435                 440                 445             


Ser Pro Val Val Lys Arg Thr Phe Gly Gln Ala Ile Asn Leu Ile Asn 
    450                 455                 460                 


Lys Ile Ile Glu Lys Tyr Gly Val Pro Glu Asp Ile Ile Ile Glu Leu 
465                 470                 475                 480 


Ala Arg Glu Asn Asn Ser Lys Asp Lys Gln Lys Phe Ile Asn Glu Met 
                485                 490                 495     


Gln Lys Lys Asn Glu Asn Thr Arg Lys Arg Ile Asn Glu Ile Ile Gly 
            500                 505                 510         


Lys Tyr Gly Asn Gln Asn Ala Lys Arg Leu Val Glu Lys Ile Arg Leu 
        515                 520                 525             


His Asp Glu Gln Glu Gly Lys Cys Leu Tyr Ser Leu Glu Ser Ile Pro 
    530                 535                 540                 


Leu Glu Asp Leu Leu Asn Asn Pro Asn His Tyr Glu Val Asp His Ile 
545                 550                 555                 560 


Ile Pro Arg Ser Val Ser Phe Asp Asn Ser Tyr His Asn Lys Val Leu 
                565                 570                 575     


Val Lys Gln Ser Glu Asn Ser Lys Lys Ser Asn Leu Thr Pro Tyr Gln 
            580                 585                 590         


Tyr Phe Asn Ser Gly Lys Ser Lys Leu Ser Tyr Asn Gln Phe Lys Gln 
        595                 600                 605             


His Ile Leu Asn Leu Ser Lys Ser Gln Asp Arg Ile Ser Lys Lys Lys 
    610                 615                 620                 


Lys Glu Tyr Leu Leu Glu Glu Arg Asp Ile Asn Lys Phe Glu Val Gln 
625                 630                 635                 640 


Lys Glu Phe Ile Asn Arg Asn Leu Val Asp Thr Arg Tyr Ala Thr Arg 
                645                 650                 655     


Glu Leu Thr Asn Tyr Leu Lys Ala Tyr Phe Ser Ala Asn Asn Met Asn 
            660                 665                 670         


Val Lys Val Lys Thr Ile Asn Gly Ser Phe Thr Asp Tyr Leu Arg Lys 
        675                 680                 685             


Val Trp Lys Phe Lys Lys Glu Arg Asn His Gly Tyr Lys His His Ala 
    690                 695                 700                 


Glu Asp Ala Leu Ile Ile Ala Asn Ala Asp Phe Leu Phe Lys Glu Asn 
705                 710                 715                 720 


Lys Lys Leu Lys Ala Val Asn Ser Val Leu Glu Lys Pro Glu Ile Glu 
                725                 730                 735     


Thr Lys Gln Leu Asp Ile Gln Val Asp Ser Glu Asp Asn Tyr Ser Glu 
            740                 745                 750         


Met Phe Ile Ile Pro Lys Gln Val Gln Asp Ile Lys Asp Phe Arg Asn 
        755                 760                 765             


Phe Lys Tyr Ser His Arg Val Asp Lys Lys Pro Asn Arg Gln Leu Ile 
    770                 775                 780                 


Asn Asp Thr Leu Tyr Ser Thr Arg Lys Lys Asp Asn Ser Thr Tyr Ile 
785                 790                 795                 800 


Val Gln Thr Ile Lys Asp Ile Tyr Ala Lys Asp Asn Thr Thr Leu Lys 
                805                 810                 815     


Lys Gln Phe Asp Lys Ser Pro Glu Lys Phe Leu Met Tyr Gln His Asp 
            820                 825                 830         


Pro Arg Thr Phe Glu Lys Leu Glu Val Ile Met Lys Gln Tyr Ala Asn 
        835                 840                 845             


Glu Lys Asn Pro Leu Ala Lys Tyr His Glu Glu Thr Gly Glu Tyr Leu 
    850                 855                 860                 


Thr Lys Tyr Ser Lys Lys Asn Asn Gly Pro Ile Val Lys Ser Leu Lys 
865                 870                 875                 880 


Tyr Ile Gly Asn Lys Leu Gly Ser His Leu Asp Val Thr His Gln Phe 
                885                 890                 895     


Lys Ser Ser Thr Lys Lys Leu Val Lys Leu Ser Ile Lys Pro Tyr Arg 
            900                 905                 910         


Phe Asp Val Tyr Leu Thr Asp Lys Gly Tyr Lys Phe Ile Thr Ile Ser 
        915                 920                 925             


Tyr Leu Asp Val Leu Lys Lys Asp Asn Tyr Tyr Tyr Ile Pro Glu Gln 
    930                 935                 940                 


Lys Tyr Asp Lys Leu Lys Leu Gly Lys Ala Ile Asp Lys Asn Ala Lys 
945                 950                 955                 960 


Phe Ile Ala Ser Phe Tyr Lys Asn Asp Leu Ile Lys Leu Asp Gly Glu 
                965                 970                 975     


Ile Tyr Lys Ile Ile Gly Val Asn Ser Asp Thr Arg Asn Met Ile Glu 
            980                 985                 990         


Leu Asp Leu Pro Asp Ile Arg Tyr  Lys Glu Tyr Cys Glu  Leu Asn Asn 
        995                 1000                 1005             


Ile Lys  Gly Glu Pro Arg Ile  Lys Lys Thr Ile Gly  Lys Lys Val 
    1010                 1015                 1020             


Asn Ser  Ile Glu Lys Leu Thr  Thr Asp Val Leu Gly  Asn Val Phe 
    1025                 1030                 1035             


Thr Asn  Thr Gln Tyr Thr Lys  Pro Gln Leu Leu Phe  Lys Arg Gly 
    1040                 1045                 1050             


Asn Gly  Gly 
    1055     


<210>  8
<211>  1057
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(1057)
<223>  MGib11SpaCas9-3E410A

<400>  8

Met Asn Gln Lys Phe Ile Leu Gly Leu Asp Ile Gly Ile Thr Ser Val 
1               5                   10                  15      


Gly Tyr Gly Leu Ile Asp Tyr Glu Thr Lys Asn Ile Ile Asp Ala Gly 
            20                  25                  30          


Val Arg Leu Phe Pro Glu Ala Asn Val Glu Asn Asn Glu Gly Arg Arg 
        35                  40                  45              


Ser Lys Arg Gly Ser Arg Arg Leu Lys Arg Arg Arg Ile His Arg Leu 
    50                  55                  60                  


Glu Arg Val Lys Leu Leu Leu Thr Glu Tyr Asp Leu Ile Asn Lys Glu 
65                  70                  75                  80  


Gln Ile Pro Thr Ser Asn Asn Pro Tyr Gln Ile Arg Val Lys Gly Leu 
                85                  90                  95      


Ser Glu Ile Leu Ser Lys Asp Glu Leu Ala Ile Ala Leu Leu His Leu 
            100                 105                 110         


Ala Lys Arg Arg Gly Ile His Asn Val Asp Val Ala Ala Asp Lys Glu 
        115                 120                 125             


Glu Thr Ala Ser Asp Ser Leu Ser Thr Lys Asp Gln Ile Asn Lys Asn 
    130                 135                 140                 


Ala Lys Phe Leu Glu Ser Arg Tyr Val Cys Glu Leu Gln Lys Glu Arg 
145                 150                 155                 160 


Leu Glu Asn Glu Gly His Val Arg Gly Val Glu Asn Arg Phe Leu Thr 
                165                 170                 175     


Lys Asp Ile Val Arg Glu Ala Lys Lys Ile Ile Asp Thr Gln Met Gln 
            180                 185                 190         


Tyr Tyr Pro Glu Ile Asp Glu Thr Phe Lys Glu Lys Tyr Ile Ser Leu 
        195                 200                 205             


Val Glu Thr Arg Arg Glu Tyr Phe Glu Gly Pro Gly Gln Gly Ser Pro 
    210                 215                 220                 


Phe Gly Trp Asn Gly Asp Leu Lys Lys Trp Tyr Glu Met Leu Met Gly 
225                 230                 235                 240 


His Cys Thr Tyr Phe Pro Gln Glu Leu Arg Ser Val Lys Tyr Ala Tyr 
                245                 250                 255     


Ser Ala Asp Leu Phe Asn Ala Leu Asn Asp Leu Asn Asn Leu Ile Ile 
            260                 265                 270         


Gln Arg Asp Asn Ser Glu Lys Leu Glu Tyr His Glu Lys Tyr His Ile 
        275                 280                 285             


Ile Glu Asn Val Phe Lys Gln Lys Lys Lys Pro Thr Leu Lys Gln Ile 
    290                 295                 300                 


Ala Lys Glu Ile Gly Val Asn Pro Glu Asp Ile Lys Gly Tyr Arg Ile 
305                 310                 315                 320 


Thr Lys Ser Gly Thr Pro Glu Phe Thr Ser Phe Lys Leu Phe His Asp 
                325                 330                 335     


Leu Lys Lys Val Val Lys Asp His Ala Ile Leu Asp Asp Ile Asp Leu 
            340                 345                 350         


Leu Asn Gln Ile Ala Glu Ile Leu Thr Ile Tyr Gln Asp Lys Asp Ser 
        355                 360                 365             


Ile Val Ala Glu Leu Gly Gln Leu Glu Tyr Leu Met Ser Glu Ala Asp 
    370                 375                 380                 


Lys Gln Ser Ile Ser Glu Leu Thr Gly Tyr Thr Gly Thr His Ser Leu 
385                 390                 395                 400 


Ser Leu Lys Cys Met Asn Met Ile Ile Asp Ala Leu Trp His Ser Ser 
                405                 410                 415     


Met Asn Gln Met Glu Val Phe Thr Tyr Leu Asn Met Arg Pro Lys Lys 
            420                 425                 430         


Tyr Glu Leu Lys Gly Tyr Gln Arg Ile Pro Thr Asp Met Ile Asp Asp 
        435                 440                 445             


Ala Ile Leu Ser Pro Val Val Lys Arg Thr Phe Ile Gln Ser Ile Asn 
    450                 455                 460                 


Val Ile Asn Lys Val Ile Glu Lys Tyr Gly Ile Pro Glu Asp Ile Ile 
465                 470                 475                 480 


Ile Glu Leu Ala Arg Glu Asn Asn Ser Asp Asp Arg Lys Lys Phe Ile 
                485                 490                 495     


Asn Asn Leu Gln Lys Lys Asn Glu Ala Thr Arg Lys Arg Ile Asn Glu 
            500                 505                 510         


Ile Ile Gly Gln Thr Gly Asn Gln Asn Ala Lys Arg Ile Val Glu Lys 
        515                 520                 525             


Ile Arg Leu His Asp Gln Gln Glu Gly Lys Cys Leu Tyr Ser Leu Glu 
    530                 535                 540                 


Ser Ile Pro Leu Glu Asp Leu Leu Asn Asn Pro Asn His Tyr Glu Val 
545                 550                 555                 560 


Asp His Ile Ile Pro Arg Ser Val Ser Phe Asp Asn Ser Tyr His Asn 
                565                 570                 575     


Lys Val Leu Val Lys Gln Ser Glu Asn Ser Lys Lys Ser Asn Leu Thr 
            580                 585                 590         


Pro Tyr Gln Tyr Phe Asn Ser Gly Lys Ser Lys Leu Ser Tyr Asn Gln 
        595                 600                 605             


Phe Lys Gln His Ile Leu Asn Leu Ser Lys Ser Gln Asp Arg Ile Ser 
    610                 615                 620                 


Lys Lys Lys Lys Glu Tyr Leu Leu Glu Glu Arg Asp Ile Asn Lys Phe 
625                 630                 635                 640 


Glu Val Gln Lys Glu Phe Ile Asn Arg Asn Leu Val Asp Thr Arg Tyr 
                645                 650                 655     


Ala Thr Arg Glu Leu Thr Ser Tyr Leu Lys Ala Tyr Phe Ser Ala Asn 
            660                 665                 670         


Asn Met Asp Val Lys Val Lys Thr Ile Asn Gly Ser Phe Thr Asn His 
        675                 680                 685             


Leu Arg Lys Val Trp Arg Phe Asp Lys Tyr Arg Asn His Gly Tyr Lys 
    690                 695                 700                 


His His Ala Glu Asp Ala Leu Ile Ile Ala Asn Ala Asp Phe Leu Phe 
705                 710                 715                 720 


Lys Glu Asn Lys Lys Leu Gln Asn Thr Asn Lys Ile Leu Glu Lys Pro 
                725                 730                 735     


Thr Ile Glu Asn Asn Thr Lys Lys Val Thr Val Glu Lys Glu Glu Asp 
            740                 745                 750         


Tyr Asn Asn Val Phe Glu Thr Pro Lys Leu Val Glu Asp Ile Lys Gln 
        755                 760                 765             


Tyr Arg Asp Tyr Lys Phe Ser His Arg Val Asp Lys Lys Pro Asn Arg 
    770                 775                 780                 


Gln Leu Ile Asn Asp Thr Leu Tyr Ser Thr Arg Met Lys Asp Glu His 
785                 790                 795                 800 


Asp Tyr Ile Val Gln Thr Ile Thr Asp Ile Tyr Gly Lys Asp Asn Thr 
                805                 810                 815     


Asn Leu Lys Lys Gln Phe Asn Lys Asn Pro Glu Lys Phe Leu Met Tyr 
            820                 825                 830         


Gln Asn Asp Pro Lys Thr Phe Glu Lys Leu Ser Ile Ile Met Lys Gln 
        835                 840                 845             


Tyr Ser Asp Glu Lys Asn Pro Leu Ala Lys Tyr Tyr Glu Glu Thr Gly 
    850                 855                 860                 


Glu Tyr Leu Thr Lys Tyr Ser Lys Lys Asn Asn Gly Pro Ile Val Lys 
865                 870                 875                 880 


Lys Ile Lys Leu Leu Gly Asn Lys Val Gly Asn His Leu Asp Val Thr 
                885                 890                 895     


Asn Lys Tyr Glu Asn Ser Thr Lys Lys Leu Val Lys Leu Ser Ile Lys 
            900                 905                 910         


Asn Tyr Arg Phe Asp Val Tyr Leu Thr Glu Lys Gly Tyr Lys Phe Val 
        915                 920                 925             


Thr Ile Ala Tyr Leu Asn Val Phe Lys Lys Asp Asn Tyr Tyr Tyr Ile 
    930                 935                 940                 


Pro Lys Asp Lys Tyr Gln Glu Leu Lys Glu Lys Lys Lys Ile Lys Asp 
945                 950                 955                 960 


Thr Asp Gln Phe Ile Ala Ser Phe Tyr Lys Asn Asp Leu Ile Lys Leu 
                965                 970                 975     


Asn Gly Asp Leu Tyr Lys Ile Ile Gly Val Asn Ser Asp Asp Arg Asn 
            980                 985                 990         


Ile Ile Glu Leu Asp Tyr Tyr Asp  Ile Lys Tyr Lys Asp  Tyr Cys Glu 
        995                 1000                 1005             


Ile Asn  Asn Ile Lys Gly Glu  Pro Arg Ile Lys Lys  Thr Ile Gly 
    1010                 1015                 1020             


Lys Lys  Thr Glu Ser Ile Glu  Lys Phe Thr Thr Asp  Val Leu Gly 
    1025                 1030                 1035             


Asn Leu  Tyr Leu His Ser Thr  Glu Lys Ala Pro Gln  Leu Ile Phe 
    1040                 1045                 1050             


Lys Arg  Gly Leu 
    1055         


<210>  9
<211>  1056
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(1056)
<223>  SluCas9

<400>  9

Met Asn Gln Lys Phe Ile Leu Gly Leu Asp Ile Gly Ile Thr Ser Val 
1               5                   10                  15      


Gly Tyr Gly Leu Ile Asp Tyr Glu Thr Lys Asn Ile Ile Asp Ala Gly 
            20                  25                  30          


Val Arg Leu Phe Pro Glu Ala Asn Val Glu Asn Asn Glu Gly Arg Arg 
        35                  40                  45              


Ser Lys Arg Gly Ser Arg Arg Leu Lys Arg Arg Arg Ile His Arg Leu 
    50                  55                  60                  


Glu Arg Val Lys Lys Leu Leu Glu Asp Tyr Asn Leu Leu Asp Gln Ser 
65                  70                  75                  80  


Gln Ile Pro Gln Ser Thr Asn Pro Tyr Ala Ile Arg Val Lys Gly Leu 
                85                  90                  95      


Ser Glu Ala Leu Ser Lys Asp Glu Leu Val Ile Ala Leu Leu His Ile 
            100                 105                 110         


Ala Lys Arg Arg Gly Ile His Lys Ile Asp Val Ile Asp Ser Asn Asp 
        115                 120                 125             


Asp Val Gly Asn Glu Leu Ser Thr Lys Glu Gln Leu Asn Lys Asn Ser 
    130                 135                 140                 


Lys Leu Leu Lys Asp Lys Phe Val Cys Gln Ile Gln Leu Glu Arg Met 
145                 150                 155                 160 


Asn Glu Gly Gln Val Arg Gly Glu Lys Asn Arg Phe Lys Thr Ala Asp 
                165                 170                 175     


Ile Ile Lys Glu Ile Ile Gln Leu Leu Asn Val Gln Lys Asn Phe His 
            180                 185                 190         


Gln Leu Asp Glu Asn Phe Ile Asn Lys Tyr Ile Glu Leu Val Glu Met 
        195                 200                 205             


Arg Arg Glu Tyr Phe Glu Gly Pro Gly Lys Gly Ser Pro Tyr Gly Trp 
    210                 215                 220                 


Glu Gly Asp Pro Lys Ala Trp Tyr Glu Thr Leu Met Gly His Cys Thr 
225                 230                 235                 240 


Tyr Phe Pro Asp Glu Leu Arg Ser Val Lys Tyr Ala Tyr Ser Ala Asp 
                245                 250                 255     


Leu Phe Asn Ala Leu Asn Asp Leu Asn Asn Leu Val Ile Gln Arg Asp 
            260                 265                 270         


Gly Leu Ser Lys Leu Glu Tyr His Glu Lys Tyr His Ile Ile Glu Asn 
        275                 280                 285             


Val Phe Lys Gln Lys Lys Lys Pro Thr Leu Lys Gln Ile Ala Asn Glu 
    290                 295                 300                 


Ile Asn Val Asn Pro Glu Asp Ile Lys Gly Tyr Arg Ile Thr Lys Ser 
305                 310                 315                 320 


Gly Lys Pro Gln Phe Thr Glu Phe Lys Leu Tyr His Asp Leu Lys Ser 
                325                 330                 335     


Val Leu Phe Asp Gln Ser Ile Leu Glu Asn Glu Asp Val Leu Asp Gln 
            340                 345                 350         


Ile Ala Glu Ile Leu Thr Ile Tyr Gln Asp Lys Asp Ser Ile Lys Ser 
        355                 360                 365             


Lys Leu Thr Glu Leu Asp Ile Leu Leu Asn Glu Glu Asp Lys Glu Asn 
    370                 375                 380                 


Ile Ala Gln Leu Thr Gly Tyr Thr Gly Thr His Arg Leu Ser Leu Lys 
385                 390                 395                 400 


Cys Ile Arg Leu Val Leu Glu Glu Gln Trp Tyr Ser Ser Arg Asn Gln 
                405                 410                 415     


Met Glu Ile Phe Thr His Leu Asn Ile Lys Pro Lys Lys Ile Asn Leu 
            420                 425                 430         


Thr Ala Ala Asn Lys Ile Pro Lys Ala Met Ile Asp Glu Phe Ile Leu 
        435                 440                 445             


Ser Pro Val Val Lys Arg Thr Phe Gly Gln Ala Ile Asn Leu Ile Asn 
    450                 455                 460                 


Lys Ile Ile Glu Lys Tyr Gly Val Pro Glu Asp Ile Ile Ile Glu Leu 
465                 470                 475                 480 


Ala Arg Glu Asn Asn Ser Lys Asp Lys Gln Lys Phe Ile Asn Glu Met 
                485                 490                 495     


Gln Lys Lys Asn Glu Asn Thr Arg Lys Arg Ile Asn Glu Ile Ile Gly 
            500                 505                 510         


Lys Tyr Gly Asn Gln Asn Ala Lys Arg Leu Val Glu Lys Ile Arg Leu 
        515                 520                 525             


His Asp Glu Gln Glu Gly Lys Cys Leu Tyr Ser Leu Glu Ser Ile Pro 
    530                 535                 540                 


Leu Glu Asp Leu Leu Asn Asn Pro Asn His Tyr Glu Val Asp His Ile 
545                 550                 555                 560 


Ile Pro Arg Ser Val Ser Phe Asp Asn Ser Tyr His Asn Lys Val Leu 
                565                 570                 575     


Val Lys Gln Ser Glu Asn Ser Lys Lys Ser Asn Leu Thr Pro Tyr Gln 
            580                 585                 590         


Tyr Phe Asn Ser Gly Lys Ser Lys Leu Ser Tyr Asn Gln Phe Lys Gln 
        595                 600                 605             


His Ile Leu Asn Leu Ser Lys Ser Gln Asp Arg Ile Ser Lys Lys Lys 
    610                 615                 620                 


Lys Glu Tyr Leu Leu Glu Glu Arg Asp Ile Asn Lys Phe Glu Val Gln 
625                 630                 635                 640 


Lys Glu Phe Ile Asn Arg Asn Leu Val Asp Thr Arg Tyr Ala Thr Arg 
                645                 650                 655     


Glu Leu Thr Asn Tyr Leu Lys Ala Tyr Phe Ser Ala Asn Asn Met Asn 
            660                 665                 670         


Val Lys Val Lys Thr Ile Asn Gly Ser Phe Thr Asp Tyr Leu Arg Lys 
        675                 680                 685             


Val Trp Lys Phe Lys Lys Glu Arg Asn His Gly Tyr Lys His His Ala 
    690                 695                 700                 


Glu Asp Ala Leu Ile Ile Ala Asn Ala Asp Phe Leu Phe Lys Glu Asn 
705                 710                 715                 720 


Lys Lys Leu Lys Ala Val Asn Ser Val Leu Glu Lys Pro Glu Ile Glu 
                725                 730                 735     


Thr Lys Gln Leu Asp Ile Gln Val Asp Ser Glu Asp Asn Tyr Ser Glu 
            740                 745                 750         


Met Phe Ile Ile Pro Lys Gln Val Gln Asp Ile Lys Asp Phe Arg Asn 
        755                 760                 765             


Phe Lys Tyr Ser His Arg Val Asp Lys Lys Pro Asn Arg Gln Leu Ile 
    770                 775                 780                 


Asn Asp Thr Leu Tyr Ser Thr Arg Lys Lys Asp Asn Ser Thr Tyr Ile 
785                 790                 795                 800 


Val Gln Thr Ile Lys Asp Ile Tyr Ala Lys Asp Asn Thr Thr Leu Lys 
                805                 810                 815     


Lys Gln Phe Asp Lys Ser Pro Glu Lys Phe Leu Met Tyr Gln His Asp 
            820                 825                 830         


Pro Arg Thr Phe Glu Lys Leu Glu Val Ile Met Lys Gln Tyr Ala Asn 
        835                 840                 845             


Glu Lys Asn Pro Leu Ala Lys Tyr His Glu Glu Thr Gly Glu Tyr Leu 
    850                 855                 860                 


Thr Lys Tyr Ser Lys Lys Asn Asn Gly Pro Ile Val Lys Ser Leu Lys 
865                 870                 875                 880 


Tyr Ile Gly Asn Lys Leu Gly Ser His Leu Asp Val Thr His Gln Phe 
                885                 890                 895     


Lys Ser Ser Thr Lys Lys Leu Val Lys Leu Ser Ile Lys Pro Tyr Arg 
            900                 905                 910         


Phe Asp Val Tyr Leu Thr Asp Lys Gly Tyr Lys Phe Ile Thr Ile Ser 
        915                 920                 925             


Tyr Leu Asp Val Leu Lys Lys Asp Asn Tyr Tyr Tyr Ile Pro Glu Gln 
    930                 935                 940                 


Lys Tyr Asp Lys Leu Lys Leu Gly Lys Ala Ile Asp Lys Asn Ala Lys 
945                 950                 955                 960 


Phe Ile Ala Ser Phe Tyr Lys Asn Asp Leu Ile Lys Leu Asp Gly Glu 
                965                 970                 975     


Ile Tyr Lys Ile Ile Gly Val Asn Ser Asp Thr Arg Asn Met Ile Glu 
            980                 985                 990         


Leu Asp Leu Pro Asp Ile Arg Tyr  Lys Glu Tyr Cys Glu  Leu Asn Asn 
        995                 1000                 1005             


Ile Lys  Gly Glu Pro Arg Ile  Lys Lys Thr Ile Gly  Lys Lys Val 
    1010                 1015                 1020             


Asn Ser  Ile Glu Lys Leu Thr  Thr Asp Val Leu Gly  Asn Val Phe 
    1025                 1030                 1035             


Thr Asn  Thr Gln Tyr Thr Lys  Pro Gln Leu Leu Phe  Lys Arg Gly 
    1040                 1045                 1050             


Asn Gly  Gly 
    1055     


<210>  10
<211>  150
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(150)
<223>  sgRNA_Ex1

<400>  10
ggugaacgug gaugaaguug guuuuaguac ucuggaaaca gaaucuacug aaacaagaca       60

auaugucgug uuuaucccau caauuuauug gugggauuuu uuucuagcau aaccccuugg      120

ggccucuaaa cgggucuuga gggguuuuuu                                       150


<210>  11
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(28)
<223>  R01-1-A

<400>  11
ggtgaacgtg gatgaagttg tggggtac                                          28


<210>  12
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(33)
<223>  R01-1-B

<400>  12
gcggtacccc acaacttcat ccacgttcac cgg                                    33


<210>  13
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(28)
<223>  R01-2-A

<400>  13
agtgaacgtg gatgaagttg tggggtac                                          28


<210>  14
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(33)
<223>  R01-2-B

<400>  14
gcggtacccc acaacttcat ccacgttcac tgg                                    33


<210>  15
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(28)
<223>  R01-3-A

<400>  15
cgtgaacgtg gatgaagttg tggggtac                                          28


<210>  16
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(33)
<223>  R01-3-B

<400>  16
gcggtacccc acaacttcat ccacgttcac ggg                                    33


<210>  17
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(28)
<223>  R01-4-A

<400>  17
tgtgaacgtg gatgaagttg tggggtac                                          28


<210>  18
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(33)
<223>  R01-4-B

<400>  18
gcggtacccc acaacttcat ccacgttcac agg                                    33


<210>  19
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(28)
<223>  R01-5-A

<400>  19
gatgaacgtg gatgaagttg tggggtac                                          28


<210>  20
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(33)
<223>  R01-5-B

<400>  20
gcggtacccc acaacttcat ccacgttcat cgg                                    33


<210>  21
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(28)
<223>  R01-6-A

<400>  21
gctgaacgtg gatgaagttg tggggtac                                          28


<210>  22
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(33)
<223>  R01-6-B

<400>  22
gcggtacccc acaacttcat ccacgttcag cgg                                    33


<210>  23
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(28)
<223>  R01-7-A

<400>  23
gttgaacgtg gatgaagttg tggggtac                                          28


<210>  24
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(33)
<223>  R01-7-B

<400>  24
gcggtacccc acaacttcat ccacgttcaa cgg                                    33


<210>  25
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(28)
<223>  R01-8-A

<400>  25
ggagaacgtg gatgaagttg tggggtac                                          28


<210>  26
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(33)
<223>  R01-8-B

<400>  26
gcggtacccc acaacttcat ccacgttctc cgg                                    33


<210>  27
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(28)
<223>  R01-9-A

<400>  27
ggcgaacgtg gatgaagttg tggggtac                                          28


<210>  28
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(33)
<223>  R01-9-B

<400>  28
gcggtacccc acaacttcat ccacgttcgc cgg                                    33


<210>  29
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(28)
<223>  R01-10-A

<400>  29
ggggaacgtg gatgaagttg tggggtac                                          28


<210>  30
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(33)
<223>  R01-10-B

<400>  30
gcggtacccc acaacttcat ccacgttccc cgg                                    33


<210>  31
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(28)
<223>  R01-11-A

<400>  31
ggtaaacgtg gatgaagttg tggggtac                                          28


<210>  32
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(33)
<223>  R01-11-B

<400>  32
gcggtacccc acaacttcat ccacgtttac cgg                                    33


<210>  33
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(28)
<223>  R01-12-A

<400>  33
ggtcaacgtg gatgaagttg tggggtac                                          28


<210>  34
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(33)
<223>  R01-12-B

<400>  34
gcggtacccc acaacttcat ccacgttgac cgg                                    33


<210>  35
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(28)
<223>  R01-13-A

<400>  35
ggttaacgtg gatgaagttg tggggtac                                          28


<210>  36
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(33)
<223>  R01-13-B

<400>  36
gcggtacccc acaacttcat ccacgttaac cgg                                    33


<210>  37
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(28)
<223>  R01-14-A

<400>  37
ggtgcacgtg gatgaagttg tggggtac                                          28


<210>  38
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(33)
<223>  R01-14-B

<400>  38
gcggtacccc acaacttcat ccacgtgcac cgg                                    33


<210>  39
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(28)
<223>  R01-15-A

<400>  39
ggtgtacgtg gatgaagttg tggggtac                                          28


<210>  40
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(33)
<223>  R01-15-B

<400>  40
gcggtacccc acaacttcat ccacgtacac cgg                                    33


<210>  41
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(28)
<223>  R01-16-A

<400>  41
ggtggacgtg gatgaagttg tggggtac                                          28


<210>  42
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(33)
<223>  R01-16-B

<400>  42
gcggtacccc acaacttcat ccacgtccac cgg                                    33


<210>  43
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(28)
<223>  R01-17-A

<400>  43
ggtgaccgtg gatgaagttg tggggtac                                          28


<210>  44
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(33)
<223>  R01-17-B

<400>  44
gcggtacccc acaacttcat ccacggtcac cgg                                    33


<210>  45
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(28)
<223>  R01-18-A

<400>  45
ggtgatcgtg gatgaagttg tggggtac                                          28


<210>  46
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(33)
<223>  R01-18-B

<400>  46
gcggtacccc acaacttcat ccacgatcac cgg                                    33


<210>  47
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(28)
<223>  R01-19-A

<400>  47
ggtgagcgtg gatgaagttg tggggtac                                          28


<210>  48
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(33)
<223>  R01-19-B

<400>  48
gcggtacccc acaacttcat ccacgctcac cgg                                    33


<210>  49
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(28)
<223>  R01-20-A

<400>  49
ggtgaaagtg gatgaagttg tggggtac                                          28


<210>  50
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(33)
<223>  R01-20-B

<400>  50
gcggtacccc acaacttcat ccactttcac cgg                                    33


<210>  51
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(28)
<223>  R01-21-A

<400>  51
ggtgaatgtg gatgaagttg tggggtac                                          28


<210>  52
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(33)
<223>  R01-21-B

<400>  52
gcggtacccc acaacttcat ccacattcac cgg                                    33


<210>  53
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(28)
<223>  R01-22-A

<400>  53
ggtgaaggtg gatgaagttg tggggtac                                          28


<210>  54
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(33)
<223>  R01-22-B

<400>  54
gcggtacccc acaacttcat ccaccttcac cgg                                    33


<210>  55
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(28)
<223>  R01-23-A

<400>  55
ggtgaacatg gatgaagttg tggggtac                                          28


<210>  56
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(33)
<223>  R01-23-B

<400>  56
gcggtacccc acaacttcat ccatgttcac cgg                                    33


<210>  57
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(28)
<223>  R01-24-A

<400>  57
ggtgaacctg gatgaagttg tggggtac                                          28


<210>  58
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(33)
<223>  R01-24-B

<400>  58
gcggtacccc acaacttcat ccaggttcac cgg                                    33


<210>  59
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(28)
<223>  R01-25-A

<400>  59
ggtgaacttg gatgaagttg tggggtac                                          28


<210>  60
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(33)
<223>  R01-25-B

<400>  60
gcggtacccc acaacttcat ccaagttcac cgg                                    33


<210>  61
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(28)
<223>  R01-26-A

<400>  61
ggtgaacgag gatgaagttg tggggtac                                          28


<210>  62
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(33)
<223>  R01-26-B

<400>  62
gcggtacccc acaacttcat cctcgttcac cgg                                    33


<210>  63
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(28)
<223>  R01-27-A

<400>  63
ggtgaacgcg gatgaagttg tggggtac                                          28


<210>  64
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(33)
<223>  R01-27-B

<400>  64
gcggtacccc acaacttcat ccgcgttcac cgg                                    33


<210>  65
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(28)
<223>  R01-28-A

<400>  65
ggtgaacggg gatgaagttg tggggtac                                          28


<210>  66
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(33)
<223>  R01-28-B

<400>  66
gcggtacccc acaacttcat ccccgttcac cgg                                    33


<210>  67
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(28)
<223>  R01-29-A

<400>  67
ggtgaacgta gatgaagttg tggggtac                                          28


<210>  68
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(33)
<223>  R01-29-B

<400>  68
gcggtacccc acaacttcat ctacgttcac cgg                                    33


<210>  69
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(28)
<223>  R01-30-A

<400>  69
ggtgaacgtc gatgaagttg tggggtac                                          28


<210>  70
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(33)
<223>  R01-30-B

<400>  70
gcggtacccc acaacttcat cgacgttcac cgg                                    33


<210>  71
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(28)
<223>  R01-31-A

<400>  71
ggtgaacgtt gatgaagttg tggggtac                                          28


<210>  72
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(33)
<223>  R01-31-B

<400>  72
gcggtacccc acaacttcat caacgttcac cgg                                    33


<210>  73
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(28)
<223>  R01-32-A

<400>  73
ggtgaacgtg aatgaagttg tggggtac                                          28


<210>  74
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(33)
<223>  R01-32-B

<400>  74
gcggtacccc acaacttcat tcacgttcac cgg                                    33


<210>  75
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(28)
<223>  R01-33-A

<400>  75
ggtgaacgtg catgaagttg tggggtac                                          28


<210>  76
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(33)
<223>  R01-33-B

<400>  76
gcggtacccc acaacttcat gcacgttcac cgg                                    33


<210>  77
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(28)
<223>  R01-34-A

<400>  77
ggtgaacgtg tatgaagttg tggggtac                                          28


<210>  78
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(33)
<223>  R01-34-B

<400>  78
gcggtacccc acaacttcat acacgttcac cgg                                    33


<210>  79
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(28)
<223>  R01-35-A

<400>  79
ggtgaacgtg gctgaagttg tggggtac                                          28


<210>  80
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(33)
<223>  R01-35-B

<400>  80
gcggtacccc acaacttcag ccacgttcac cgg                                    33


<210>  81
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(28)
<223>  R01-36-A

<400>  81
ggtgaacgtg gttgaagttg tggggtac                                          28


<210>  82
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(33)
<223>  R01-36-B

<400>  82
gcggtacccc acaacttcaa ccacgttcac cgg                                    33


<210>  83
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(28)
<223>  R01-37-A

<400>  83
ggtgaacgtg ggtgaagttg tggggtac                                          28


<210>  84
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(33)
<223>  R01-37-B

<400>  84
gcggtacccc acaacttcac ccacgttcac cgg                                    33


<210>  85
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(28)
<223>  R01-38-A

<400>  85
ggtgaacgtg gaagaagttg tggggtac                                          28


<210>  86
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(33)
<223>  R01-38-B

<400>  86
gcggtacccc acaacttctt ccacgttcac cgg                                    33


<210>  87
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(28)
<223>  R01-39-A

<400>  87
ggtgaacgtg gacgaagttg tggggtac                                          28


<210>  88
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(33)
<223>  R01-39-B

<400>  88
gcggtacccc acaacttcgt ccacgttcac cgg                                    33


<210>  89
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(28)
<223>  R01-40-A

<400>  89
ggtgaacgtg gaggaagttg tggggtac                                          28


<210>  90
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(33)
<223>  R01-40-B

<400>  90
gcggtacccc acaacttcct ccacgttcac cgg                                    33


<210>  91
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(28)
<223>  R01-41-A

<400>  91
ggtgaacgtg gataaagttg tggggtac                                          28


<210>  92
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(33)
<223>  R01-41-B

<400>  92
gcggtacccc acaactttat ccacgttcac cgg                                    33


<210>  93
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(28)
<223>  R01-42-A

<400>  93
ggtgaacgtg gatcaagttg tggggtac                                          28


<210>  94
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(33)
<223>  R01-42-B

<400>  94
gcggtacccc acaacttgat ccacgttcac cgg                                    33


<210>  95
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(28)
<223>  R01-43-A

<400>  95
ggtgaacgtg gattaagttg tggggtac                                          28


<210>  96
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(33)
<223>  R01-43-B

<400>  96
gcggtacccc acaacttaat ccacgttcac cgg                                    33


<210>  97
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(28)
<223>  R01-44-A

<400>  97
ggtgaacgtg gatgcagttg tggggtac                                          28


<210>  98
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(33)
<223>  R01-44-B

<400>  98
gcggtacccc acaactgcat ccacgttcac cgg                                    33


<210>  99
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(28)
<223>  R01-45-A

<400>  99
ggtgaacgtg gatgtagttg tggggtac                                          28


<210>  100
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(33)
<223>  R01-45-B

<400>  100
gcggtacccc acaactacat ccacgttcac cgg                                    33


<210>  101
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(28)
<223>  R01-46-A

<400>  101
ggtgaacgtg gatggagttg tggggtac                                          28


<210>  102
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(33)
<223>  R01-46-B

<400>  102
gcggtacccc acaactccat ccacgttcac cgg                                    33


<210>  103
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(28)
<223>  R01-47-A

<400>  103
ggtgaacgtg gatgacgttg tggggtac                                          28


<210>  104
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(33)
<223>  R01-47-B

<400>  104
gcggtacccc acaacgtcat ccacgttcac cgg                                    33


<210>  105
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(28)
<223>  R01-48-A

<400>  105
ggtgaacgtg gatgatgttg tggggtac                                          28


<210>  106
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(33)
<223>  R01-48-B

<400>  106
gcggtacccc acaacatcat ccacgttcac cgg                                    33


<210>  107
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(28)
<223>  R01-49-A

<400>  107
ggtgaacgtg gatgaggttg tggggtac                                          28


<210>  108
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(33)
<223>  R01-49-B

<400>  108
gcggtacccc acaacctcat ccacgttcac cgg                                    33


<210>  109
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(27)
<223>  R01-50-A

<400>  109
ggtgaacgtg gatgaaattg tggggta                                           27


<210>  110
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(33)
<223>  R01-50-B

<400>  110
gcggtacccc acaatttcat ccacgttcac cgg                                    33


<210>  111
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(28)
<223>  R01-51-A

<400>  111
ggtgaacgtg gatgaacttg tggggtac                                          28


<210>  112
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(33)
<223>  R01-51-B

<400>  112
gcggtacccc acaagttcat ccacgttcac cgg                                    33


<210>  113
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(28)
<223>  R01-52-A

<400>  113
ggtgaacgtg gatgaatttg tggggtac                                          28


<210>  114
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(33)
<223>  R01-52-B

<400>  114
gcggtacccc acaaattcat ccacgttcac cgg                                    33


<210>  115
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(28)
<223>  R01-53-A

<400>  115
ggtgaacgtg gatgaagatg tggggtac                                          28


<210>  116
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(33)
<223>  R01-53-B

<400>  116
gcggtacccc acatcttcat ccacgttcac cgg                                    33


<210>  117
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(28)
<223>  R01-54-A

<400>  117
ggtgaacgtg gatgaagctg tggggtac                                          28


<210>  118
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(33)
<223>  R01-54-B

<400>  118
gcggtacccc acagcttcat ccacgttcac cgg                                    33


<210>  119
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(28)
<223>  R01-55-A

<400>  119
ggtgaacgtg gatgaaggtg tggggtac                                          28


<210>  120
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(33)
<223>  R01-55-B

<400>  120
gcggtacccc acaccttcat ccacgttcac cgg                                    33


<210>  121
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(28)
<223>  R01-56-A

<400>  121
ggtgaacgtg gatgaagtag tggggtac                                          28


<210>  122
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(33)
<223>  R01-56-B

<400>  122
gcggtacccc actacttcat ccacgttcac cgg                                    33


<210>  123
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(28)
<223>  R01-57-A

<400>  123
ggtgaacgtg gatgaagtcg tggggtac                                          28


<210>  124
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(33)
<223>  R01-57-B

<400>  124
gcggtacccc acgacttcat ccacgttcac cgg                                    33


<210>  125
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(28)
<223>  R01-58-A

<400>  125
ggtgaacgtg gatgaagtgg tggggtac                                          28


<210>  126
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(33)
<223>  R01-58-B

<400>  126
gcggtacccc accacttcat ccacgttcac cgg                                    33


<210>  127
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(28)
<223>  R01-59-A

<400>  127
ggtgaacgtg gatgaagtta tggggtac                                          28


<210>  128
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(33)
<223>  R01-59-B

<400>  128
gcggtacccc ataacttcat ccacgttcac cgg                                    33


<210>  129
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(28)
<223>  R01-60-A

<400>  129
ggtgaacgtg gatgaagttc tggggtac                                          28


<210>  130
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(33)
<223>  R01-60-B

<400>  130
gcggtacccc agaacttcat ccacgttcac cgg                                    33


<210>  131
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(28)
<223>  R01-61-A

<400>  131
ggtgaacgtg gatgaagttt tggggtac                                          28


<210>  132
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(33)
<223>  R01-61-B

<400>  132
gcggtacccc aaaacttcat ccacgttcac cgg                                    33


<210>  133
<211>  1057
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(1057)
<223>  Gib11SpaCas9-1-M417L

<400>  133

Met Asn Gln Lys Phe Ile Leu Gly Leu Asp Ile Gly Ile Thr Ser Val 
1               5                   10                  15      


Gly Tyr Gly Leu Ile Asp Tyr Glu Thr Lys Asn Ile Ile Asp Ala Gly 
            20                  25                  30          


Val Arg Leu Phe Pro Glu Ala Asn Val Glu Asn Asn Glu Gly Arg Arg 
        35                  40                  45              


Ser Lys Arg Gly Ser Arg Arg Leu Lys Arg Arg Arg Ile His Arg Leu 
    50                  55                  60                  


Glu Arg Val Lys Leu Leu Leu Thr Glu Tyr Asp Leu Ile Asn Lys Glu 
65                  70                  75                  80  


Gln Ile Pro Thr Ser Asn Asn Pro Tyr Gln Ile Arg Val Lys Gly Leu 
                85                  90                  95      


Ser Glu Ile Leu Ser Lys Asp Glu Leu Ala Ile Ala Leu Leu His Leu 
            100                 105                 110         


Ala Lys Arg Arg Gly Ile His Asn Val Asp Val Ala Ala Asp Lys Glu 
        115                 120                 125             


Glu Thr Ala Ser Asp Ser Leu Ser Thr Lys Asp Gln Ile Asn Lys Asn 
    130                 135                 140                 


Ala Lys Phe Leu Glu Ser Arg Tyr Val Cys Glu Leu Gln Lys Glu Arg 
145                 150                 155                 160 


Leu Glu Asn Glu Gly His Val Arg Gly Val Glu Asn Arg Phe Leu Thr 
                165                 170                 175     


Lys Asp Ile Val Arg Glu Ala Lys Lys Ile Ile Asp Thr Gln Met Gln 
            180                 185                 190         


Tyr Tyr Pro Glu Ile Asp Glu Thr Phe Lys Glu Lys Tyr Ile Ser Leu 
        195                 200                 205             


Val Glu Thr Arg Arg Glu Tyr Phe Glu Gly Pro Gly Gln Gly Ser Pro 
    210                 215                 220                 


Phe Gly Trp Asn Gly Asp Leu Lys Lys Trp Tyr Glu Met Leu Met Gly 
225                 230                 235                 240 


His Cys Thr Tyr Phe Pro Gln Glu Leu Arg Ser Val Lys Tyr Ala Tyr 
                245                 250                 255     


Ser Ala Asp Leu Phe Asn Ala Leu Asn Asp Leu Asn Asn Leu Ile Ile 
            260                 265                 270         


Gln Arg Asp Asn Ser Glu Lys Leu Glu Tyr His Glu Lys Tyr His Ile 
        275                 280                 285             


Ile Glu Asn Val Phe Lys Gln Lys Lys Lys Pro Thr Leu Lys Gln Ile 
    290                 295                 300                 


Ala Lys Glu Ile Gly Val Asn Pro Glu Asp Ile Lys Gly Tyr Arg Ile 
305                 310                 315                 320 


Thr Lys Ser Gly Thr Pro Glu Phe Thr Ser Phe Lys Leu Phe His Asp 
                325                 330                 335     


Leu Lys Lys Val Val Lys Asp His Ala Ile Leu Asp Asp Ile Asp Leu 
            340                 345                 350         


Leu Asn Gln Ile Ala Glu Ile Leu Thr Ile Tyr Gln Asp Lys Asp Ser 
        355                 360                 365             


Ile Val Ala Glu Leu Gly Gln Leu Glu Tyr Leu Met Ser Glu Ala Asp 
    370                 375                 380                 


Lys Gln Ser Ile Ser Glu Leu Thr Gly Tyr Thr Gly Thr His Ser Leu 
385                 390                 395                 400 


Ser Leu Lys Cys Met Asn Met Ile Ile Asp Glu Leu Trp His Ser Ser 
                405                 410                 415     


Leu Asn Gln Met Glu Val Phe Thr Tyr Leu Asn Met Arg Pro Lys Lys 
            420                 425                 430         


Tyr Glu Leu Lys Gly Tyr Gln Arg Ile Pro Thr Asp Met Ile Asp Asp 
        435                 440                 445             


Ala Ile Leu Ser Pro Val Val Lys Arg Thr Phe Ile Gln Ser Ile Asn 
    450                 455                 460                 


Val Ile Asn Lys Val Ile Glu Lys Tyr Gly Ile Pro Glu Asp Ile Ile 
465                 470                 475                 480 


Ile Glu Leu Ala Arg Glu Asn Asn Ser Asp Asp Arg Lys Lys Phe Ile 
                485                 490                 495     


Asn Asn Leu Gln Lys Lys Asn Glu Ala Thr Arg Lys Arg Ile Asn Glu 
            500                 505                 510         


Ile Ile Gly Gln Thr Gly Asn Gln Asn Ala Lys Arg Ile Val Glu Lys 
        515                 520                 525             


Ile Arg Leu His Asp Gln Gln Glu Gly Lys Cys Leu Tyr Ser Leu Glu 
    530                 535                 540                 


Ser Ile Pro Leu Glu Asp Leu Leu Asn Asn Pro Asn His Tyr Glu Val 
545                 550                 555                 560 


Asp His Ile Ile Pro Arg Ser Val Ser Phe Asp Asn Ser Tyr His Asn 
                565                 570                 575     


Lys Val Leu Val Lys Gln Ser Glu Asn Ser Lys Lys Ser Asn Leu Thr 
            580                 585                 590         


Pro Tyr Gln Tyr Phe Asn Ser Gly Lys Ser Lys Leu Ser Tyr Asn Gln 
        595                 600                 605             


Phe Lys Gln His Ile Leu Asn Leu Ser Lys Ser Gln Asp Arg Ile Ser 
    610                 615                 620                 


Lys Lys Lys Lys Glu Tyr Leu Leu Glu Glu Arg Asp Ile Asn Lys Phe 
625                 630                 635                 640 


Glu Val Gln Lys Glu Phe Ile Asn Arg Asn Leu Val Asp Thr Arg Tyr 
                645                 650                 655     


Ala Thr Arg Glu Leu Thr Asn Tyr Leu Lys Ala Tyr Phe Ser Ala Asn 
            660                 665                 670         


Asn Met Asn Val Lys Val Lys Thr Ile Asn Gly Ser Phe Thr Asp Tyr 
        675                 680                 685             


Leu Arg Lys Val Trp Lys Phe Lys Lys Glu Arg Asn His Gly Tyr Lys 
    690                 695                 700                 


His His Ala Glu Asp Ala Leu Ile Ile Ala Asn Ala Asp Phe Leu Phe 
705                 710                 715                 720 


Lys Glu Asn Lys Lys Leu Lys Ala Val Asn Ser Val Leu Glu Lys Pro 
                725                 730                 735     


Glu Ile Glu Thr Lys Gln Leu Asp Ile Gln Val Asp Ser Glu Asp Asn 
            740                 745                 750         


Tyr Ser Glu Met Phe Ile Ile Pro Lys Gln Val Gln Asp Ile Lys Asp 
        755                 760                 765             


Phe Arg Asn Phe Lys Tyr Ser His Arg Val Asp Lys Lys Pro Asn Arg 
    770                 775                 780                 


Gln Leu Ile Asn Asp Thr Leu Tyr Ser Thr Arg Lys Lys Asp Asn Ser 
785                 790                 795                 800 


Thr Tyr Ile Val Gln Thr Ile Lys Asp Ile Tyr Ala Lys Asp Asn Thr 
                805                 810                 815     


Thr Leu Lys Lys Gln Phe Asp Lys Ser Pro Glu Lys Phe Leu Met Tyr 
            820                 825                 830         


Gln His Asp Pro Arg Thr Phe Glu Lys Leu Glu Val Ile Met Lys Gln 
        835                 840                 845             


Tyr Ala Asn Glu Lys Asn Pro Leu Ala Lys Tyr His Glu Glu Thr Gly 
    850                 855                 860                 


Glu Tyr Leu Thr Lys Tyr Ser Lys Lys Asn Asn Gly Pro Ile Val Lys 
865                 870                 875                 880 


Ser Leu Lys Tyr Ile Gly Asn Lys Leu Gly Ser His Leu Asp Val Thr 
                885                 890                 895     


His Gln Phe Lys Ser Ser Thr Lys Lys Leu Val Lys Leu Ser Ile Lys 
            900                 905                 910         


Asn Tyr Arg Phe Asp Val Tyr Leu Thr Glu Lys Gly Tyr Lys Phe Val 
        915                 920                 925             


Thr Ile Ala Tyr Leu Asn Val Phe Lys Lys Asp Asn Tyr Tyr Tyr Ile 
    930                 935                 940                 


Pro Lys Asp Lys Tyr Gln Glu Leu Lys Glu Lys Lys Lys Ile Lys Asp 
945                 950                 955                 960 


Thr Asp Gln Phe Ile Ala Ser Phe Tyr Lys Asn Asp Leu Ile Lys Leu 
                965                 970                 975     


Asn Gly Asp Leu Tyr Lys Ile Ile Gly Val Asn Ser Asp Asp Arg Asn 
            980                 985                 990         


Ile Ile Glu Leu Asp Tyr Tyr Asp  Ile Lys Tyr Lys Asp  Tyr Cys Glu 
        995                 1000                 1005             


Ile Asn  Asn Ile Lys Gly Glu  Pro Arg Ile Lys Lys  Thr Ile Gly 
    1010                 1015                 1020             


Lys Lys  Thr Glu Ser Ile Glu  Lys Phe Thr Thr Asp  Val Leu Gly 
    1025                 1030                 1035             


Asn Leu  Tyr Leu His Ser Thr  Glu Lys Ala Pro Gln  Leu Ile Phe 
    1040                 1045                 1050             


Lys Arg  Gly Leu 
    1055         


