                         SEQUENCE LISTING

<110>  Academisch Ziekenhuis Leiden (h.o.d.n. LUMC)
 
<120>  Optimised RAG1 deficient SCID Gene Therapy

<130>  P267488NL

<140>  2022714
<141>  2019-03-11

<160>  44    

<170>  PatentIn version 3.5

<210>  1
<211>  1043
<212>  PRT
<213>  Homo sapiens

<400>  1

Met Ala Ala Ser Phe Pro Pro Thr Leu Gly Leu Ser Ser Ala Pro Asp 
1               5                   10                  15      


Glu Ile Gln His Pro His Ile Lys Phe Ser Glu Trp Lys Phe Lys Leu 
            20                  25                  30          


Phe Arg Val Arg Ser Phe Glu Lys Thr Pro Glu Glu Ala Gln Lys Glu 
        35                  40                  45              


Lys Lys Asp Ser Phe Glu Gly Lys Pro Ser Leu Glu Gln Ser Pro Ala 
    50                  55                  60                  


Val Leu Asp Lys Ala Asp Gly Gln Lys Pro Val Pro Thr Gln Pro Leu 
65                  70                  75                  80  


Leu Lys Ala His Pro Lys Phe Ser Lys Lys Phe His Asp Asn Glu Lys 
                85                  90                  95      


Ala Arg Gly Lys Ala Ile His Gln Ala Asn Leu Arg His Leu Cys Arg 
            100                 105                 110         


Ile Cys Gly Asn Ser Phe Arg Ala Asp Glu His Asn Arg Arg Tyr Pro 
        115                 120                 125             


Val His Gly Pro Val Asp Gly Lys Thr Leu Gly Leu Leu Arg Lys Lys 
    130                 135                 140                 


Glu Lys Arg Ala Thr Ser Trp Pro Asp Leu Ile Ala Lys Val Phe Arg 
145                 150                 155                 160 


Ile Asp Val Lys Ala Asp Val Asp Ser Ile His Pro Thr Glu Phe Cys 
                165                 170                 175     


His Asn Cys Trp Ser Ile Met His Arg Lys Phe Ser Ser Ala Pro Cys 
            180                 185                 190         


Glu Val Tyr Phe Pro Arg Asn Val Thr Met Glu Trp His Pro His Thr 
        195                 200                 205             


Pro Ser Cys Asp Ile Cys Asn Thr Ala Arg Arg Gly Leu Lys Arg Lys 
    210                 215                 220                 


Ser Leu Gln Pro Asn Leu Gln Leu Ser Lys Lys Leu Lys Thr Val Leu 
225                 230                 235                 240 


Asp Gln Ala Arg Gln Ala Arg Gln His Lys Arg Arg Ala Gln Ala Arg 
                245                 250                 255     


Ile Ser Ser Lys Asp Val Met Lys Lys Ile Ala Asn Cys Ser Lys Ile 
            260                 265                 270         


His Leu Ser Thr Lys Leu Leu Ala Val Asp Phe Pro Glu His Phe Val 
        275                 280                 285             


Lys Ser Ile Ser Cys Gln Ile Cys Glu His Ile Leu Ala Asp Pro Val 
    290                 295                 300                 


Glu Thr Asn Cys Lys His Val Phe Cys Arg Val Cys Ile Leu Arg Cys 
305                 310                 315                 320 


Leu Lys Val Met Gly Ser Tyr Cys Pro Ser Cys Arg Tyr Pro Cys Phe 
                325                 330                 335     


Pro Thr Asp Leu Glu Ser Pro Val Lys Ser Phe Leu Ser Val Leu Asn 
            340                 345                 350         


Ser Leu Met Val Lys Cys Pro Ala Lys Glu Cys Asn Glu Glu Val Ser 
        355                 360                 365             


Leu Glu Lys Tyr Asn His His Ile Ser Ser His Lys Glu Ser Lys Glu 
    370                 375                 380                 


Ile Phe Val His Ile Asn Lys Gly Gly Arg Pro Arg Gln His Leu Leu 
385                 390                 395                 400 


Ser Leu Thr Arg Arg Ala Gln Lys His Arg Leu Arg Glu Leu Lys Leu 
                405                 410                 415     


Gln Val Lys Ala Phe Ala Asp Lys Glu Glu Gly Gly Asp Val Lys Ser 
            420                 425                 430         


Val Cys Met Thr Leu Phe Leu Leu Ala Leu Arg Ala Arg Asn Glu His 
        435                 440                 445             


Arg Gln Ala Asp Glu Leu Glu Ala Ile Met Gln Gly Lys Gly Ser Gly 
    450                 455                 460                 


Leu Gln Pro Ala Val Cys Leu Ala Ile Arg Val Asn Thr Phe Leu Ser 
465                 470                 475                 480 


Cys Ser Gln Tyr His Lys Met Tyr Arg Thr Val Lys Ala Ile Thr Gly 
                485                 490                 495     


Arg Gln Ile Phe Gln Pro Leu His Ala Leu Arg Asn Ala Glu Lys Val 
            500                 505                 510         


Leu Leu Pro Gly Tyr His His Phe Glu Trp Gln Pro Pro Leu Lys Asn 
        515                 520                 525             


Val Ser Ser Ser Thr Asp Val Gly Ile Ile Asp Gly Leu Ser Gly Leu 
    530                 535                 540                 


Ser Ser Ser Val Asp Asp Tyr Pro Val Asp Thr Ile Ala Lys Arg Phe 
545                 550                 555                 560 


Arg Tyr Asp Ser Ala Leu Val Ser Ala Leu Met Asp Met Glu Glu Asp 
                565                 570                 575     


Ile Leu Glu Gly Met Arg Ser Gln Asp Leu Asp Asp Tyr Leu Asn Gly 
            580                 585                 590         


Pro Phe Thr Val Val Val Lys Glu Ser Cys Asp Gly Met Gly Asp Val 
        595                 600                 605             


Ser Glu Lys His Gly Ser Gly Pro Val Val Pro Glu Lys Ala Val Arg 
    610                 615                 620                 


Phe Ser Phe Thr Ile Met Lys Ile Thr Ile Ala His Ser Ser Gln Asn 
625                 630                 635                 640 


Val Lys Val Phe Glu Glu Ala Lys Pro Asn Ser Glu Leu Cys Cys Lys 
                645                 650                 655     


Pro Leu Cys Leu Met Leu Ala Asp Glu Ser Asp His Glu Thr Leu Thr 
            660                 665                 670         


Ala Ile Leu Ser Pro Leu Ile Ala Glu Arg Glu Ala Met Lys Ser Ser 
        675                 680                 685             


Glu Leu Met Leu Glu Leu Gly Gly Ile Leu Arg Thr Phe Lys Phe Ile 
    690                 695                 700                 


Phe Arg Gly Thr Gly Tyr Asp Glu Lys Leu Val Arg Glu Val Glu Gly 
705                 710                 715                 720 


Leu Glu Ala Ser Gly Ser Val Tyr Ile Cys Thr Leu Cys Asp Ala Thr 
                725                 730                 735     


Arg Leu Glu Ala Ser Gln Asn Leu Val Phe His Ser Ile Thr Arg Ser 
            740                 745                 750         


His Ala Glu Asn Leu Glu Arg Tyr Glu Val Trp Arg Ser Asn Pro Tyr 
        755                 760                 765             


His Glu Ser Val Glu Glu Leu Arg Asp Arg Val Lys Gly Val Ser Ala 
    770                 775                 780                 


Lys Pro Phe Ile Glu Thr Val Pro Ser Ile Asp Ala Leu His Cys Asp 
785                 790                 795                 800 


Ile Gly Asn Ala Ala Glu Phe Tyr Lys Ile Phe Gln Leu Glu Ile Gly 
                805                 810                 815     


Glu Val Tyr Lys Asn Pro Asn Ala Ser Lys Glu Glu Arg Lys Arg Trp 
            820                 825                 830         


Gln Ala Thr Leu Asp Lys His Leu Arg Lys Lys Met Asn Leu Lys Pro 
        835                 840                 845             


Ile Met Arg Met Asn Gly Asn Phe Ala Arg Lys Leu Met Thr Lys Glu 
    850                 855                 860                 


Thr Val Asp Ala Val Cys Glu Leu Ile Pro Ser Glu Glu Arg His Glu 
865                 870                 875                 880 


Ala Leu Arg Glu Leu Met Asp Leu Tyr Leu Lys Met Lys Pro Val Trp 
                885                 890                 895     


Arg Ser Ser Cys Pro Ala Lys Glu Cys Pro Glu Ser Leu Cys Gln Tyr 
            900                 905                 910         


Ser Phe Asn Ser Gln Arg Phe Ala Glu Leu Leu Ser Thr Lys Phe Lys 
        915                 920                 925             


Tyr Arg Tyr Glu Gly Lys Ile Thr Asn Tyr Phe His Lys Thr Leu Ala 
    930                 935                 940                 


His Val Pro Glu Ile Ile Glu Arg Asp Gly Ser Ile Gly Ala Trp Ala 
945                 950                 955                 960 


Ser Glu Gly Asn Glu Ser Gly Asn Lys Leu Phe Arg Arg Phe Arg Lys 
                965                 970                 975     


Met Asn Ala Arg Gln Ser Lys Cys Tyr Glu Met Glu Asp Val Leu Lys 
            980                 985                 990         


His His Trp Leu Tyr Thr Ser Lys  Tyr Leu Gln Lys Phe  Met Asn Ala 
        995                 1000                 1005             


His Asn  Ala Leu Lys Thr Ser  Gly Phe Thr Met Asn  Pro Gln Ala 
    1010                 1015                 1020             


Ser Leu  Gly Asp Pro Leu Gly  Ile Glu Asp Ser Leu  Glu Ser Gln 
    1025                 1030                 1035             


Asp Ser  Met Glu Phe 
    1040             


<210>  2
<211>  1051
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  codon optimised nucleic acid sequence encoding human RAG1 
       catalytic domain

<400>  2
caagggcggc agaccccggc agcacctgct gtccctgacc agacgggccc agaagcaccg       60

gctgcgggag ctgaagctcc aggtcaaggc cttcgccgac aaagaggaag gcggcgacgt      120

caagagcgtg tgcatgaccc tgtttctgct ggccctgcgg gccaggaacg agcaccggca      180

ggccgatgag ctggaagcca tcatgcaggg caagggcagc ggcctccagc ctgccgtgtg      240

cctggccatc cgggtgaaca cctttctgag ctgtagccag taccacaaga tgtaccggac      300

cgtgaaggcc atcaccggca gacagatctt ccagcctctg cacgccctgc ggaacgccga      360

gaaggtgctg ctgcccggct accaccactt cgagtggcag ccccccctga agaacgtgag      420

cagcagcacc gacgtgggca tcatcgacgg cctgagcggc ctgtccagca gcgtggacga      480

ctaccctgtg gacaccatcg ccaagcggtt cagatacgac agcgccctgg tgtccgccct      540

gatggacatg gaagaggaca tcctggaagg catgcggagc caggacctgg acgattacct      600

gaacggcccc ttcaccgtgg tggtgaaaga gtcctgcgac ggcatgggcg acgtgagcga      660

gaagcacggc agcggccctg tggtgcccga gaaggccgtg cggttcagct tcaccatcat      720

gaagatcacc atcgcccaca gcagccagaa cgtgaaggtg ttcgaggaag ccaagcccaa      780

cagcgagctg tgctgcaagc ccctgtgcct gatgctggcc gacgagagcg accacgagac      840

cctgaccgcc atcctgagcc ccctgatcgc cgagcgggag gccatgaaga gcagcgaact      900

gatgctggaa ctgggcggca tcctgaggac cttcaagttc atcttccggg gcaccggcta      960

cgacgagaag ctggtccggg aggtggaggg cctggaagcc agcggcagcg tgtacatctg     1020

caccctgtgc gacgccaccc ggctggaagc c                                    1051


<210>  3
<211>  3132
<212>  DNA
<213>  Artificial Sequence

<220>
<223>   RAG1 cDNA sequence

<400>  3
atggcagcct ctttcccacc caccttggga ctcagttctg ccccagatga aattcagcac       60

ccacatatta aattttcaga atggaaattt aagctgttcc gggtgagatc ctttgaaaag      120

acacctgaag aagctcaaaa ggaaaagaag gattcctttg aggggaaacc ctctctggag      180

caatctccag cagtcctgga caaggctgat ggtcagaagc cagtcccaac tcagccattg      240

ttaaaagccc accctaagtt ttcaaagaaa tttcacgaca acgagaaagc aagaggcaaa      300

gcgatccatc aagccaacct tcgacatctc tgccgcatct gtgggaattc ttttagagct      360

gatgagcaca acaggagata tccagtccat ggtcctgtgg atggtaaaac cctaggcctt      420

ttacgaaaga aggaaaagag agctacttcc tggccggacc tcattgccaa ggttttccgg      480

atcgatgtga aggcagatgt tgactcgatc caccccactg agttctgcca taactgctgg      540

agcatcatgc acaggaagtt tagcagtgcc ccatgtgagg tttacttccc gaggaacgtg      600

accatggagt ggcaccccca cacaccatcc tgtgacatct gcaacactgc ccgtcgggga      660

ctcaagagga agagtcttca gccaaacttg cagctcagca aaaaactcaa aactgtgctt      720

gaccaagcaa gacaagcccg tcagcgcaag agaagagctc aggcaaggat cagcagcaag      780

gatgtcatga agaagatcgc caactgcagt aagatacatc ttagtaccaa gctccttgca      840

gtggacttcc cagagcactt tgtgaaatcc atctcctgcc agatctgtga acacattctg      900

gctgaccctg tggagaccaa ctgtaagcat gtcttttgcc gggtctgcat tctcagatgc      960

ctcaaagtca tgggcagcta ttgtccctct tgccgatatc catgcttccc tactgacctg     1020

gagagtccag tgaagtcctt tctgagcgtc ttgaattccc tgatggtgaa atgtccagca     1080

aaagagtgca atgaggaggt cagtttggaa aaatataatc accacatctc aagtcacaag     1140

gaatcaaaag agatttttgt gcacattaat aaagggggcc ggccccgcca acatcttctg     1200

tcgctgactc ggagagctca gaagcaccgg ctgagggagc tcaagctgca agtcaaagcc     1260

tttgctgaca aagaagaagg tggagatgtg aagtccgtgt gcatgacctt gttcctgctg     1320

gctctgaggg cgaggaatga gcacaggcaa gctgatgagc tggaggccat catgcaggga     1380

aagggctctg gcctgcagcc agctgtttgc ttggccatcc gtgtcaacac cttcctcagc     1440

tgcagtcagt accacaagat gtacaggact gtgaaagcca tcacagggag acagattttt     1500

cagcctttgc atgcccttcg gaatgctgag aaggtacttc tgccaggcta ccaccacttt     1560

gagtggcagc cacctctgaa gaatgtgtct tccagcactg atgttggcat tattgatggg     1620

ctgtctggac tatcatcctc tgtggatgat tacccagtgg acaccattgc aaagaggttc     1680

cgctatgatt cagctttggt gtctgctttg atggacatgg aagaagacat cttggaaggc     1740

atgagatccc aagaccttga tgattacctg aatggcccct tcactgtggt ggtgaaggag     1800

tcttgtgatg gaatgggaga cgtgagtgag aagcatggga gtgggcctgt agttccagaa     1860

aaggcagtcc gtttttcatt cacaatcatg aaaattacta ttgcccacag ctctcagaat     1920

gtgaaagtat ttgaagaagc caaacctaac tctgaactgt gttgcaagcc attgtgcctt     1980

atgctggcag atgagtctga ccacgagacg ctgactgcca tcctgagtcc tctcattgct     2040

gagagggagg ccatgaagag cagtgaatta atgcttgagc tgggaggcat tctccggact     2100

ttcaagttca tcttcagggg caccggctat gatgaaaaac ttgtgcggga agtggaaggc     2160

ctcgaggctt ctggctcagt ctacatttgt actctttgtg atgccacccg tctggaagcc     2220

tctcaaaatc ttgtcttcca ctctataacc agaagccatg ctgagaacct ggaacgttat     2280

gaggtctggc gttccaaccc ttaccatgag tctgtggaag aactgcggga tcgggtgaaa     2340

ggggtctcag ctaaaccttt cattgagaca gtcccttcca tagatgcact ccactgtgac     2400

attggcaatg cagctgagtt ctacaagatc ttccagctag agatagggga agtgtataag     2460

aatcccaatg cttccaaaga ggaaaggaaa aggtggcagg ccacactgga caagcatctc     2520

cggaagaaga tgaacctcaa accaatcatg aggatgaatg gcaactttgc caggaagctc     2580

atgaccaaag agactgtgga tgcagtttgt gagttaattc cttccgagga gaggcacgag     2640

gctctgaggg agctgatgga tctttacctg aagatgaaac cagtatggcg atcatcatgc     2700

cctgctaaag agtgcccaga atccctctgc cagtacagtt tcaattcaca gcgttttgct     2760

gagctccttt ctacgaagtt caagtatagg tatgagggaa aaatcaccaa ttattttcac     2820

aaaaccctgg cccatgttcc tgaaattatt gagagggatg gctccattgg ggcatgggca     2880

agtgagggaa atgagtctgg taacaaactg tttaggcgct tccggaaaat gaatgccagg     2940

cagtccaaat gctatgagat ggaagatgtc ctgaaacacc actggttgta cacctccaaa     3000

tacctccaga agtttatgaa tgctcataat gcattaaaaa cctctgggtt taccatgaac     3060

cctcaggcaa gcttagggga cccattaggc atagaggact ctctggaaag ccaagattca     3120

atggaatttt aa                                                         3132


<210>  4
<211>  3132
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  codon optimised RAG1 DNA sequence

<400>  4
atggccgcca gcttcccccc taccctgggc ctgagcagcg cccctgacga gatccagcac       60

ccccacatca agttcagcga gtggaagttc aagctgttca gagtgcggag cttcgagaaa      120

acccccgagg aagcccagaa agagaagaag gacagcttcg agggcaagcc cagcctggaa      180

cagagccctg ccgtgctgga caaggccgac ggccagaaac ccgtgcccac ccagcccctg      240

ctgaaggccc accccaagtt cagcaagaag ttccacgaca acgagaaggc caggggcaag      300

gccatccacc aggccaacct gcggcacctg tgccggatct gcggcaacag cttccgggcc      360

gacgagcaca accggcgcta ccccgtgcac ggccccgtgg acggcaagac actgggcctg      420

ctgcggaaga aagagaaacg ggccacctcc tggcccgacc tgatcgccaa ggtgttccgg      480

atcgacgtga aggccgacgt ggacagcatc caccccaccg agttctgcca caactgctgg      540

tccatcatgc accggaagtt cagctccgcc ccctgcgagg tgtacttccc ccggaacgtg      600

accatggaat ggcaccctca cacccccagc tgcgacatct gcaacaccgc cagacggggc      660

ctgaagcgga agagcctcca gcccaacctc cagctgtcca agaaactgaa aaccgtgctg      720

gatcaggccc ggcaggccag gcagcggaag cggagagccc aggcccggat cagcagcaag      780

gacgtgatga agaagatcgc caactgtagc aagatccacc tgagcaccaa gctgctggcc      840

gtggacttcc ccgagcactt cgtgaagagc atcagctgcc agatctgcga gcacatcctg      900

gccgaccccg tggagaccaa ctgcaagcac gtgttctgta gagtgtgcat cctgcggtgc      960

ctgaaagtga tgggcagcta ctgccccagc tgtagatacc cctgcttccc caccgacctg     1020

gaaagccccg tgaagagctt cctgagcgtg ctgaacagcc tgatggtgaa gtgccccgcc     1080

aaagagtgca acgaggaagt cagcctggaa aagtacaacc accacatcag cagccacaaa     1140

gagagcaaag aaatcttcgt ccacatcaac aagggcggca gaccccggca gcacctgctg     1200

tccctgacca gacgggccca gaagcaccgg ctgcgggagc tgaagctcca ggtcaaggcc     1260

ttcgccgaca aagaggaagg cggcgacgtc aagagcgtgt gcatgaccct gtttctgctg     1320

gccctgcggg ccaggaacga gcaccggcag gccgatgagc tggaagccat catgcagggc     1380

aagggcagcg gcctccagcc tgccgtgtgc ctggccatcc gggtgaacac ctttctgagc     1440

tgtagccagt accacaagat gtaccggacc gtgaaggcca tcaccggcag acagatcttc     1500

cagcctctgc acgccctgcg gaacgccgag aaggtgctgc tgcccggcta ccaccacttc     1560

gagtggcagc cccccctgaa gaacgtgagc agcagcaccg acgtgggcat catcgacggc     1620

ctgagcggcc tgtccagcag cgtggacgac taccctgtgg acaccatcgc caagcggttc     1680

agatacgaca gcgccctggt gtccgccctg atggacatgg aagaggacat cctggaaggc     1740

atgcggagcc aggacctgga cgattacctg aacggcccct tcaccgtggt ggtgaaagag     1800

tcctgcgacg gcatgggcga cgtgagcgag aagcacggca gcggccctgt ggtgcccgag     1860

aaggccgtgc ggttcagctt caccatcatg aagatcacca tcgcccacag cagccagaac     1920

gtgaaggtgt tcgaggaagc caagcccaac agcgagctgt gctgcaagcc cctgtgcctg     1980

atgctggccg acgagagcga ccacgagacc ctgaccgcca tcctgagccc cctgatcgcc     2040

gagcgggagg ccatgaagag cagcgaactg atgctggaac tgggcggcat cctgaggacc     2100

ttcaagttca tcttccgggg caccggctac gacgagaagc tggtccggga ggtggagggc     2160

ctggaagcca gcggcagcgt gtacatctgc accctgtgcg acgccacccg gctggaagcc     2220

tcccagaacc tggtgttcca cagcatcacc agaagccacg ccgagaacct ggaaagatac     2280

gaagtgtggc ggagcaaccc ctaccacgag agcgtggagg aactgcggga ccgggtcaag     2340

ggcgtgagcg ccaagccctt catcgagacc gtgcccagca tcgacgccct gcactgcgat     2400

atcggcaacg ccgccgagtt ctacaagatc tttcagctgg aaatcgggga ggtgtacaag     2460

aaccccaacg ccagcaaaga ggaacggaag cgctggcagg ccaccctgga caagcacctg     2520

aggaagaaaa tgaacctgaa gcccatcatg cggatgaacg gcaacttcgc tcggaagctg     2580

atgaccaaag aaaccgtgga cgccgtgtgc gagctgatcc ccagcgagga acggcacgag     2640

gccctgcgcg agctgatgga cctgtacctg aagatgaagc ccgtgtggag aagcagctgt     2700

cctgccaaag aatgccccga gagcctgtgc cagtacagct tcaacagcca gcggttcgcc     2760

gagctgctgt ccaccaagtt caagtaccgc tacgagggca agatcaccaa ctacttccac     2820

aagaccctgg cccacgtgcc cgagatcatc gagcgggacg gcagcatcgg cgcctgggcc     2880

agcgagggca acgagagcgg caacaagctg ttccggcggt tcagaaagat gaatgccagg     2940

cagagcaagt gctacgagat ggaagatgtg ctgaagcacc actggctgta caccagcaag     3000

tacctccaga aattcatgaa cgcccacaac gccctgaaaa ccagcggctt caccatgaac     3060

ccccaggcca gcctgggcga ccctctgggc atcgaggact ccctggaatc ccaggacagc     3120

atggaattct ga                                                         3132


<210>  5
<211>  399
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MND promoter sequence

<400>  5
tttatttagt ctccagaaaa aggggggaat gaaagacccc acctgtaggt ttggcaagct       60

aggatcaagg ttaggaacag agagacagca gaatatgggc caaacaggat atctgtggta      120

agcagttcct gccccggctc agggccaaga acagttggaa cagcagaata tgggccaaac      180

aggatatctg tggtaagcag ttcctgcccc ggctcagggc caagaacaga tggtccccag      240

atgcggtccc gccctcagca gtttctagag aaccatcaga tgtttccagg gtgccccaag      300

gacctgaaat gaccctgtgc cttatttgaa ctaaccaatc agttcgcttc tcgcttctgt      360

tcgcgcgctt ctgctccccg agctcaataa aagagccca                             399


<210>  6
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  6
tggagataac actctaagca taactaaagg t                                      31


<210>  7
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  7
gatgtagttg cttgggaccc a                                                 21


<210>  8
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  probe


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  FAM

<220>
<221>  misc_feature
<222>  (28)..(28)
<223>  TAMRA

<400>  8
ccatttttgg tttgggcttc acaccatt                                          28


<210>  9
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  9
caactgcaag cacgtgttct g                                                 21


<210>  10
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  10
gcagtagctg cccatcactt t                                                 21


<210>  11
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  probe


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  FAM

<220>
<221>  misc_feature
<222>  (23)..(23)
<223>  TAMRA

<400>  11
agagtgtgca tcctgcggtg cct                                               23


<210>  12
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  forward primer - PTBP2

<400>  12
tctccattcc ctatgttcat gc                                                22


<210>  13
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  reverse primer - PTBP2

<400>  13
gttcccgcag aatggtgagg tg                                                22


<210>  14
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Probe - PTBP2


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  JOE

<220>
<221>  misc_feature
<222>  (20)..(20)
<223>  BHQ1

<400>  14
atgttcctcg gaccaacttg                                                   20


<210>  15
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  forward primer - WPRE

<400>  15
gaggagttgt ggcccgttgt                                                   20


<210>  16
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  reverse primer - WPRE

<400>  16
tgacaggtgg tggcaatgcc                                                   20


<210>  17
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Probe - WPRE


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  6FAM

<220>
<221>  misc_feature
<222>  (19)..(19)
<223>  BHQ1

<400>  17
ctgtgtttgc tgacgcaac                                                    19


<210>  18
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mVb1 forward primer

<400>  18
ctgaatgccc agacagctcc aagc                                              24


<210>  19
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mVb2 forward primer

<400>  19
tcactgatac ggagctgagg c                                                 21


<210>  20
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mVb3.1 forward primer

<400>  20
ccttgcagcc tagaaattca gt                                                22


<210>  21
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mVb4 forward primer

<400>  21
gcctcaagtc gcttccaacc tc                                                22


<210>  22
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mVb5.1 forward primer

<400>  22
cattatgata aaatggagag agat                                              24


<210>  23
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mVb5.2 forward primer

<400>  23
aaggtggaga gagacaaagg attc                                              24


<210>  24
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mVb5.3 forward primer

<400>  24
agaaaggaaa cctgcctggt t                                                 21


<210>  25
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mVb6 forward primer

<400>  25
ctctcactgt gacatctgcc c                                                 21


<210>  26
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mVb7 forward primer

<400>  26
tacagggtct cacggaagaa gc                                                22


<210>  27
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mVb8.1 forward primer

<400>  27
cattactcat atgtcgctga c                                                 21


<210>  28
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mVb8.2 forward primer

<400>  28
cattattcat atggtgctgg c                                                 21


<210>  29
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mVb8.3 forward primer

<400>  29
tgctggcaac cttcgaatag ga                                                22


<210>  30
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mVb9 forward primer

<400>  30
tctctctaca ttggctctgc aggc                                              24


<210>  31
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mvb10 forward primer

<400>  31
atcaagtctg tagagccgga gga                                               23


<210>  32
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mvb11 forward primer

<400>  32
gcactcaact ctgaagatcc agagc                                             25


<210>  33
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mVb12 forward primer

<400>  33
gatggtgggg ctttcaagga tc                                                22


<210>  34
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mVb13 forward primer

<400>  34
aggcctaaag gaactaactc ccac                                              24


<210>  35
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mVb14 forward primer

<400>  35
acgaccaatt catcctaagc ac                                                22


<210>  36
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mVb15 forward primer

<400>  36
cccatcagtc atcccaactt atcc                                              24


<210>  37
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mVb16 forward primer

<400>  37
cactctgaaa atccaaccca c                                                 21


<210>  38
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mVb17 forward primer

<400>  38
agtgttcctc gaactcacag                                                   20


<210>  39
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mVb18 forward primer

<400>  39
cagccggcca aacctaacat tctc                                              24


<210>  40
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mVb19 forward primer

<400>  40
ctgctaagaa accatgtacc a                                                 21


<210>  41
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mVb20 forward primer

<400>  41
tctgcagcct gggaatcaga a                                                 21


<210>  42
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  muTCB1-FAM reverse primer


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  FAM

<400>  42
cttgggtgga gtcacatttc tc                                                22


<210>  43
<211>  10111
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pCCL.MND.coRAG1 plasmid

<400>  43
ccattgcata cgttgtatcc atatcataat atgtacattt atattggctc atgtccaaca       60

ttaccgccat gttgacattg attattgact agttattaat agtaatcaat tacggggtca      120

ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct      180

ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta      240

acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac      300

ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt      360

aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag      420

tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat      480

gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat      540

gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc      600

ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt      660

ttagtgaacc ggggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac      720

tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg      780

cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga      840

aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct      900

ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact      960

ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag     1020

cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg     1080

gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt     1140

cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct     1200

acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac     1260

cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat     1320

agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg atcttcagac     1380

ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa     1440

aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa     1500

aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta     1560

tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc     1620

agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag     1680

tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc     1740

aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt     1800

ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg     1860

agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc     1920

aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt     1980

ggaattggtt taacataaca aattggctgt ggtatataaa attattcata atgatagtag     2040

gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc     2100

agggatattc accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc     2160

ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga     2220

acggatctcg acggtatcgg ttaactttta aaagaaaagg ggggattggg gggtacagtg     2280

caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac     2340

aaattacaaa aattcaaaat tttatcgatc acgagactag cctcgacgcg tagcctcgag     2400

ctagcggtac cgatcaaggt taggaacaga gagacaggag aatatgggcc aaacaggata     2460

tctgtggtaa gcagttcctg ccccggctca gggccaagaa cagttggaac agcagaatat     2520

gggccaaaca ggatatctgt ggtaagcagt tcctgccccg gctcagggcc aagaacagat     2580

ggtccccaga tgcggtcccg ccctcagcag tttctagaga accatcagat gtttccaggg     2640

tgccccaagg acctgaaatg accctgtgcc ttatttgaac taaccaatca gttcgcttct     2700

cgcttctgtt cgcgcgcttc tgctccccga gctcaataaa agagcccaca acccctcact     2760

cggcgcgcca gtcctccgat agactgcgtc gcccgggatc caccggtgcc accatggccg     2820

ccagcttccc ccctaccctg ggcctgagca gcgcccctga cgagatccag cacccccaca     2880

tcaagttcag cgagtggaag ttcaagctgt tcagagtgcg gagcttcgag aaaacccccg     2940

aggaagccca gaaagagaag aaggacagct tcgagggcaa gcccagcctg gaacagagcc     3000

ctgccgtgct ggacaaggcc gacggccaga aacccgtgcc cacccagccc ctgctgaagg     3060

cccaccccaa gttcagcaag aagttccacg acaacgagaa ggccaggggc aaggccatcc     3120

accaggccaa cctgcggcac ctgtgccgga tctgcggcaa cagcttccgg gccgacgagc     3180

acaaccggcg ctaccccgtg cacggccccg tggacggcaa gacactgggc ctgctgcgga     3240

agaaagagaa acgggccacc tcctggcccg acctgatcgc caaggtgttc cggatcgacg     3300

tgaaggccga cgtggacagc atccacccca ccgagttctg ccacaactgc tggtccatca     3360

tgcaccggaa gttcagctcc gccccctgcg aggtgtactt cccccggaac gtgaccatgg     3420

aatggcaccc tcacaccccc agctgcgaca tctgcaacac cgccagacgg ggcctgaagc     3480

ggaagagcct ccagcccaac ctccagctgt ccaagaaact gaaaaccgtg ctggatcagg     3540

cccggcaggc caggcagcgg aagcggagag cccaggcccg gatcagcagc aaggacgtga     3600

tgaagaagat cgccaactgt agcaagatcc acctgagcac caagctgctg gccgtggact     3660

tccccgagca cttcgtgaag agcatcagct gccagatctg cgagcacatc ctggccgacc     3720

ccgtggagac caactgcaag cacgtgttct gtagagtgtg catcctgcgg tgcctgaaag     3780

tgatgggcag ctactgcccc agctgtagat acccctgctt ccccaccgac ctggaaagcc     3840

ccgtgaagag cttcctgagc gtgctgaaca gcctgatggt gaagtgcccc gccaaagagt     3900

gcaacgagga agtcagcctg gaaaagtaca accaccacat cagcagccac aaagagagca     3960

aagaaatctt cgtccacatc aacaagggcg gcagaccccg gcagcacctg ctgtccctga     4020

ccagacgggc ccagaagcac cggctgcggg agctgaagct ccaggtcaag gccttcgccg     4080

acaaagagga aggcggcgac gtcaagagcg tgtgcatgac cctgtttctg ctggccctgc     4140

gggccaggaa cgagcaccgg caggccgatg agctggaagc catcatgcag ggcaagggca     4200

gcggcctcca gcctgccgtg tgcctggcca tccgggtgaa cacctttctg agctgtagcc     4260

agtaccacaa gatgtaccgg accgtgaagg ccatcaccgg cagacagatc ttccagcctc     4320

tgcacgccct gcggaacgcc gagaaggtgc tgctgcccgg ctaccaccac ttcgagtggc     4380

agccccccct gaagaacgtg agcagcagca ccgacgtggg catcatcgac ggcctgagcg     4440

gcctgtccag cagcgtggac gactaccctg tggacaccat cgccaagcgg ttcagatacg     4500

acagcgccct ggtgtccgcc ctgatggaca tggaagagga catcctggaa ggcatgcgga     4560

gccaggacct ggacgattac ctgaacggcc ccttcaccgt ggtggtgaaa gagtcctgcg     4620

acggcatggg cgacgtgagc gagaagcacg gcagcggccc tgtggtgccc gagaaggccg     4680

tgcggttcag cttcaccatc atgaagatca ccatcgccca cagcagccag aacgtgaagg     4740

tgttcgagga agccaagccc aacagcgagc tgtgctgcaa gcccctgtgc ctgatgctgg     4800

ccgacgagag cgaccacgag accctgaccg ccatcctgag ccccctgatc gccgagcggg     4860

aggccatgaa gagcagcgaa ctgatgctgg aactgggcgg catcctgagg accttcaagt     4920

tcatcttccg gggcaccggc tacgacgaga agctggtccg ggaggtggag ggcctggaag     4980

ccagcggcag cgtgtacatc tgcaccctgt gcgacgccac ccggctggaa gcctcccaga     5040

acctggtgtt ccacagcatc accagaagcc acgccgagaa cctggaaaga tacgaagtgt     5100

ggcggagcaa cccctaccac gagagcgtgg aggaactgcg ggaccgggtc aagggcgtga     5160

gcgccaagcc cttcatcgag accgtgccca gcatcgacgc cctgcactgc gatatcggca     5220

acgccgccga gttctacaag atctttcagc tggaaatcgg ggaggtgtac aagaacccca     5280

acgccagcaa agaggaacgg aagcgctggc aggccaccct ggacaagcac ctgaggaaga     5340

aaatgaacct gaagcccatc atgcggatga acggcaactt cgctcggaag ctgatgacca     5400

aagaaaccgt ggacgccgtg tgcgagctga tccccagcga ggaacggcac gaggccctgc     5460

gcgagctgat ggacctgtac ctgaagatga agcccgtgtg gagaagcagc tgtcctgcca     5520

aagaatgccc cgagagcctg tgccagtaca gcttcaacag ccagcggttc gccgagctgc     5580

tgtccaccaa gttcaagtac cgctacgagg gcaagatcac caactacttc cacaagaccc     5640

tggcccacgt gcccgagatc atcgagcggg acggcagcat cggcgcctgg gccagcgagg     5700

gcaacgagag cggcaacaag ctgttccggc ggttcagaaa gatgaatgcc aggcagagca     5760

agtgctacga gatggaagat gtgctgaagc accactggct gtacaccagc aagtacctcc     5820

agaaattcat gaacgcccac aacgccctga aaaccagcgg cttcaccatg aacccccagg     5880

ccagcctggg cgaccctctg ggcatcgagg actccctgga atcccaggac agcatggaat     5940

tctgatgagt cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc     6000

ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg     6060

ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc     6120

tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg     6180

acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg     6240

ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga     6300

caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct     6360

ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg     6420

tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc     6480

ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc     6540

cgcctggaat tcgagctcgg tacctttaag accaatgact tacaaggcag ctgtagatct     6600

tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca     6660

agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct     6720

ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca     6780

agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta     6840

gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata     6900

acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg     6960

ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc     7020

tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg     7080

cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat     7140

ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc     7200

cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg ccctatagtg     7260

agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg     7320

gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg     7380

aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg     7440

cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta     7500

cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt     7560

tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg     7620

ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat     7680

cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac     7740

tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag     7800

ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg     7860

cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg     7920

cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca     7980

ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt     8040

ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga     8100

aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga     8160

actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat     8220

gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca     8280

agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt     8340

cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac     8400

catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct     8460

aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga     8520

gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac     8580

aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat     8640

agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg     8700

ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc     8760

actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc     8820

aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg     8880

gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta     8940

atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg     9000

tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga     9060

tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt     9120

ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag     9180

agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa     9240

ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag     9300

tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca     9360

gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac     9420

cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa     9480

ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc     9540

agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg     9600

tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc     9660

ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc     9720

ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag     9780

ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa     9840

accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga     9900

ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc     9960

ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca    10020

atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta accctcacta    10080

aagggaacaa aagctggagc tgcaagcttg g                                   10111


<210>  44
<211>  286
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  beta lactamase from pCCL.MND.coRAG1 plasmid

<400>  44

Met Ser Ile Gln His Phe Arg Val Ala Leu Ile Pro Phe Phe Ala Ala 
1               5                   10                  15      


Phe Cys Leu Pro Val Phe Ala His Pro Glu Thr Leu Val Lys Val Lys 
            20                  25                  30          


Asp Ala Glu Asp Gln Leu Gly Ala Arg Val Gly Tyr Ile Glu Leu Asp 
        35                  40                  45              


Leu Asn Ser Gly Lys Ile Leu Glu Ser Phe Arg Pro Glu Glu Arg Phe 
    50                  55                  60                  


Pro Met Met Ser Thr Phe Lys Val Leu Leu Cys Gly Ala Val Leu Ser 
65                  70                  75                  80  


Arg Ile Asp Ala Gly Gln Glu Gln Leu Gly Arg Arg Ile His Tyr Ser 
                85                  90                  95      


Gln Asn Asp Leu Val Glu Tyr Ser Pro Val Thr Glu Lys His Leu Thr 
            100                 105                 110         


Asp Gly Met Thr Val Arg Glu Leu Cys Ser Ala Ala Ile Thr Met Ser 
        115                 120                 125             


Asp Asn Thr Ala Ala Asn Leu Leu Leu Thr Thr Ile Gly Gly Pro Lys 
    130                 135                 140                 


Glu Leu Thr Ala Phe Leu His Asn Met Gly Asp His Val Thr Arg Leu 
145                 150                 155                 160 


Asp Arg Trp Glu Pro Glu Leu Asn Glu Ala Ile Pro Asn Asp Glu Arg 
                165                 170                 175     


Asp Thr Thr Met Pro Val Ala Met Ala Thr Thr Leu Arg Lys Leu Leu 
            180                 185                 190         


Thr Gly Glu Leu Leu Thr Leu Ala Ser Arg Gln Gln Leu Ile Asp Trp 
        195                 200                 205             


Met Glu Ala Asp Lys Val Ala Gly Pro Leu Leu Arg Ser Ala Leu Pro 
    210                 215                 220                 


Ala Gly Trp Phe Ile Ala Asp Lys Ser Gly Ala Gly Glu Arg Gly Ser 
225                 230                 235                 240 


Arg Gly Ile Ile Ala Ala Leu Gly Pro Asp Gly Lys Pro Ser Arg Ile 
                245                 250                 255     


Val Val Ile Tyr Thr Thr Gly Ser Gln Ala Thr Met Asp Glu Arg Asn 
            260                 265                 270         


Arg Gln Ile Ala Glu Ile Gly Ala Ser Leu Ile Lys His Trp 
        275                 280                 285     


