                         SEQUENCE LISTING

<110>  UCL Business Ltd
 
<120>  MINIMAL PROMOTER

<130>  N415912WO

<150>  GB 1903499.0
<151>  2019-03-14

<160>  21    

<170>  PatentIn version 3.5

<210>  1
<211>  249
<212>  DNA
<213>  Homo sapiens

<400>  1
gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag       60

ataattagaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga      120

aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat      180

atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga      240

cgaaacacc                                                              249


<210>  2
<211>  28
<212>  DNA
<213>  Homo sapiens

<400>  2
atttcccatg attccttcat atttgcat                                          28


<210>  3
<211>  20
<212>  DNA
<213>  Homo sapiens

<400>  3
atttcccatg attccttcat                                                   20


<210>  4
<211>  8
<212>  DNA
<213>  Homo sapiens

<400>  4
atttgcat                                                                 8


<210>  5
<211>  147
<212>  DNA
<213>  Homo sapiens

<400>  5
atacgataca aggctgttag agagataatt agaattaatt tgactgtaaa cacaaagata       60

ttagtacaaa atacgtgacg tagaaagtaa taatttcttg ggtagtttgc agttttaaaa      120

ttatgtttta aaatggacta tcatatg                                          147


<210>  6
<211>  20
<212>  DNA
<213>  Homo sapiens

<400>  6
cttaccgtaa cttgaaagta                                                   20


<210>  7
<211>  17
<212>  DNA
<213>  Homo sapiens

<400>  7
tttcgatttc ttggctt                                                      17


<210>  8
<211>  111
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mini-U6 promoter of Example 5

<400>  8
gagggcctat ttcccatgat tccttcatat ttgcatatac gatagcttac cgtaacttga       60

aagtatttcg atttcttggc tttatatatc ttgtggaaag gacgaaacac c               111


<210>  9
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DSE of mini-U6 promoter of Example 5

<400>  9
atttcccatg attccttcat atttgcat                                          28


<210>  10
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SPH of mini-U6 promoter of Example 5

<400>  10
atttcccatg attccttcat                                                   20


<210>  11
<211>  8
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OCT of mini-U6 promoter of Example 5

<400>  11
atttgcat                                                                 8


<210>  12
<211>  9
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Spacer sequence connecting DSE to PSE in mini-U6 promoter of 
       Example 5

<400>  12
atacgatag                                                                9


<210>  13
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PSE of mini-U6 promoter of Example 5

<400>  13
cttaccgtaa cttgaaagta                                                   20


<210>  14
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Spacer sequence connecting PSE to TATA box in mini-U6 promoter of
       Example 5

<400>  14
tttcgatttc ttggctt                                                      17


<210>  15
<211>  353
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Wild-type human U6 promoter expressing CRISPR guide

<400>  15
gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag       60

ataattagaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga      120

aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat      180

atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga      240

cgaaacaccg tctctcagct ggtacacggc gttttagagc tagaaatagc aagttaaaat      300

aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt ttt             353


<210>  16
<211>  215
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mini-U6 promoter of Example 5 expressing CRISPR guide RNA

<400>  16
gagggcctat ttcccatgat tccttcatat ttgcatatac gatagcttac cgtaacttga       60

aagtatttcg atttcttggc tttatatatc ttgtggaaag gacgaaacac cgtctctcag      120

ctggtacacg gcgttttaga gctagaaata gcaagttaaa ataaggctag tccgttatca      180

acttgaaaaa gtggcaccga gtcggtgctt ttttt                                 215


<210>  17
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Protospacer (TRAC) in the mini-U6 promoter of Example 5

<400>  17
tctctcagct ggtacacggc                                                   20


<210>  18
<211>  76
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  cr1 scaffold

<400>  18
gttttagagc tagaaatagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt       60

ggcaccgagt cggtgc                                                       76


<210>  19
<211>  95
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  cr2 scaffold

<400>  19
gtttgagagc taagcagaaa gctgcatagc aagttcaaat aaggctagtc cgtacacaac       60

ttgaaaaagt ggcagccgag tcggctgctt ttttt                                  95


<210>  20
<211>  95
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  cr3 scaffold

<400>  20
gtttcagagc taagcacaag agtgcatagc aagttgaaat aaggctagtc cgtttacaac       60

ttgaaaaagt ggcacccgag tcgggtgctt ttttt                                  95


<210>  21
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sequence 3' of the TATA box in the mini-U6 promoter of Example 5

<400>  21
tatcttgtgg aaaggacgaa acacc                                             25


