                         SEQUENCE LISTING

<110>  Oxford Genetics Limited
 
<120>  Method of selecting antibodies

<130>  489.142378/04

<160>  8     

<170>  PatentIn version 3.5

<210>  1
<211>  299
<212>  PRT
<213>  Homo sapiens

<400>  1

Met Gln Ile Pro Gln Ala Pro Trp Pro Val Val Trp Ala Val Leu Gln 
1               5                   10                  15      


Leu Gly Trp Arg Pro Gly Trp Phe Ala Glu Gln Lys Leu Ile Ser Glu 
            20                  25                  30          


Glu Asp Leu Leu Asp Ser Pro Asp Arg Pro Trp Asn Pro Pro Thr Phe 
        35                  40                  45              


Ser Pro Ala Leu Leu Val Val Thr Glu Gly Asp Asn Ala Thr Phe Thr 
    50                  55                  60                  


Cys Ser Phe Ser Asn Thr Ser Glu Ser Phe Val Leu Asn Trp Tyr Arg 
65                  70                  75                  80  


Met Ser Pro Ser Asn Gln Thr Asp Lys Leu Ala Ala Phe Pro Glu Asp 
                85                  90                  95      


Arg Ser Gln Pro Gly Gln Asp Cys Arg Phe Arg Val Thr Gln Leu Pro 
            100                 105                 110         


Asn Gly Arg Asp Phe His Met Ser Val Val Arg Ala Arg Arg Asn Asp 
        115                 120                 125             


Ser Gly Thr Tyr Leu Cys Gly Ala Ile Ser Leu Ala Pro Lys Ala Gln 
    130                 135                 140                 


Ile Lys Glu Ser Leu Arg Ala Glu Leu Arg Val Thr Glu Arg Arg Ala 
145                 150                 155                 160 


Glu Val Pro Thr Ala His Pro Ser Pro Ser Pro Arg Pro Ala Gly Gln 
                165                 170                 175     


Phe Gln Thr Leu Val Val Gly Val Val Gly Gly Leu Leu Gly Ser Leu 
            180                 185                 190         


Val Leu Leu Val Trp Val Leu Ala Val Ile Cys Ser Arg Ala Ala Arg 
        195                 200                 205             


Gly Thr Ile Gly Ala Arg Arg Thr Gly Gln Pro Leu Lys Glu Asp Pro 
    210                 215                 220                 


Ser Ala Val Pro Val Phe Ser Val Asp Tyr Gly Glu Leu Asp Phe Gln 
225                 230                 235                 240 


Trp Arg Glu Lys Thr Pro Glu Pro Pro Val Pro Cys Val Pro Glu Gln 
                245                 250                 255     


Thr Glu Tyr Ala Thr Ile Val Phe Pro Ser Gly Met Gly Thr Ser Ser 
            260                 265                 270         


Pro Ala Arg Arg Gly Ser Ala Asp Gly Pro Arg Ser Ala Gln Pro Leu 
        275                 280                 285             


Arg Pro Glu Asp Gly His Cys Ser Trp Pro Leu 
    290                 295                 


<210>  2
<211>  10
<212>  PRT
<213>  Homo sapiens

<400>  2

Glu Gln Lys Leu Ile Ser Glu Glu Asp Leu 
1               5                   10  


<210>  3
<211>  278
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  scFv protein sequence (X indicates CDRH3 and CDRL3 sequences)


<220>
<221>  misc_feature
<222>  (117)..(124)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (237)..(249)
<223>  Xaa can be any naturally occurring amino acid

<400>  3

Met Trp Met Lys Ile Lys Thr Gly Ala Arg Ile Leu Ala Leu Ser Ala 
1               5                   10                  15      


Leu Thr Thr Met Met Phe Ser Ala Ser Ala Leu Ala Asp Ile Gln Met 
            20                  25                  30          


Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly Asp Arg Val Thr 
        35                  40                  45              


Ile Thr Cys Arg Ala Ser Gln Ser Val Ser Ser Ala Val Ala Trp Tyr 
    50                  55                  60                  


Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile Tyr Ser Ala Ser 
65                  70                  75                  80  


Ser Leu Tyr Ser Cys Val Pro Ser Arg Phe Ser Gly Ser Arg Ser Gly 
                85                  90                  95      


Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala 
            100                 105                 110         


Thr Tyr Tyr Cys Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Phe Gly Gln Gly 
        115                 120                 125             


Thr Lys Val Gly Gly Ser Ser Arg Ser Ser Glu Val Gln Leu Val Glu 
    130                 135                 140                 


Ser Gly Gly Gly Leu Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys 
145                 150                 155                 160 


Ala Ala Ser Gly Phe Asn Leu Tyr Tyr Ser Tyr Ile His Trp Val Arg 
                165                 170                 175     


Gln Ala Pro Gly Lys Gly Leu Glu Trp Val Ala Ser Ile Ser Pro Ser 
            180                 185                 190         


Tyr Gly Tyr Thr Ser Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile 
        195                 200                 205             


Ser Ala Asp Thr Ser Lys Asn Thr Ala Tyr Leu Gln Met Asn Ser Leu 
    210                 215                 220                 


Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Ala Arg Xaa Xaa Xaa Xaa 
225                 230                 235                 240 


Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Gly Phe Asp Tyr Trp Gly Gln 
                245                 250                 255     


Gly Thr Leu Val Thr Val Ser Ser Thr Ser Leu Glu Tyr Tyr Pro Tyr 
            260                 265                 270         


Asp Val Pro Asp Tyr Ala 
        275             


<210>  4
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid linker

<400>  4

Gly Gly Ser Ser Arg Ser Ser Glu Val Gln Leu Val Glu Ser Gly Gly 
1               5                   10                  15      


Gly 
    


<210>  5
<211>  297
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Pembrolizumab

<400>  5

Met Gly Trp Ser Cys Ile Ile Leu Phe Leu Val Ala Thr Ala Thr Gly 
1               5                   10                  15      


Val His Ser Glu Leu Val Met Thr Gln Val Gln Leu Val Gln Ser Gly 
            20                  25                  30          


Val Glu Val Lys Lys Pro Gly Ala Ser Val Lys Val Ser Cys Lys Ala 
        35                  40                  45              


Ser Gly Tyr Thr Phe Thr Asn Tyr Tyr Met Tyr Trp Val Arg Gln Ala 
    50                  55                  60                  


Pro Gly Gln Gly Leu Glu Trp Met Gly Gly Ile Asn Pro Ser Asn Gly 
65                  70                  75                  80  


Gly Thr Asn Phe Asn Glu Lys Phe Lys Asn Arg Val Thr Leu Thr Thr 
                85                  90                  95      


Asp Ser Ser Thr Thr Thr Ala Tyr Met Glu Leu Lys Ser Leu Gln Phe 
            100                 105                 110         


Asp Asp Thr Ala Val Tyr Tyr Cys Ala Arg Arg Asp Tyr Arg Phe Asp 
        115                 120                 125             


Met Gly Phe Asp Tyr Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser 
    130                 135                 140                 


Ala Ser Thr Lys Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
145                 150                 155                 160 


Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Ile Val 
                165                 170                 175     


Leu Thr Gln Ser Pro Ala Thr Leu Ser Leu Ser Pro Gly Glu Arg Ala 
            180                 185                 190         


Thr Leu Ser Cys Arg Ala Ser Lys Gly Val Ser Thr Ser Gly Tyr Ser 
        195                 200                 205             


Tyr Leu His Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Arg Leu Leu 
    210                 215                 220                 


Ile Tyr Leu Ala Ser Tyr Leu Glu Ser Gly Val Pro Ala Arg Phe Ser 
225                 230                 235                 240 


Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Glu 
                245                 250                 255     


Pro Glu Asp Phe Ala Val Tyr Tyr Cys Gln His Ser Arg Asp Leu Pro 
            260                 265                 270         


Leu Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys Arg Leu Glu Tyr 
        275                 280                 285             


Tyr Pro Tyr Asp Val Pro Asp Tyr Ala 
    290                 295         


<210>  6
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid linker

<400>  6

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10                  15  


<210>  7
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  7
cgatatcaaa ggaggtaccc aacatg                                            26


<210>  8
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  8
cgcataatcc ggcacatcat acggatagta c                                      31


