                        б
<110>  йѧԺо 
<120>  GLI2Ƽ;ԼGLI2Ļɸѡ
<130>  
<160>  25    
<170>  PatentIn汾3.3
<210>  1
<211>  198
<212>  PRT
<213>  ˹
<220>  
<223>  SHH Nĩź
<400>  1
Met Leu Leu Leu Leu Ala Arg Cys Phe Leu Val Ile Leu Ala Ser Ser 
1               5                   10                  15      
Leu Leu Val Cys Pro Gly Leu Ala Cys Gly Pro Gly Arg Gly Phe Gly 
            20                  25                  30          
Lys Arg Arg His Pro Lys Lys Leu Thr Pro Leu Ala Tyr Lys Gln Phe 
        35                  40                  45              
Ile Pro Asn Val Ala Glu Lys Thr Leu Gly Ala Ser Gly Arg Tyr Glu 
    50                  55                  60                  
Gly Lys Ile Thr Arg Asn Ser Glu Arg Phe Lys Glu Leu Thr Pro Asn 
65                  70                  75                  80  
Tyr Asn Pro Asp Ile Ile Phe Lys Asp Glu Glu Asn Thr Gly Ala Asp 
                85                  90                  95      
Arg Leu Met Thr Gln Arg Cys Lys Asp Lys Leu Asn Ala Leu Ala Ile 
            100                 105                 110         
Ser Val Met Asn Gln Trp Pro Gly Val Lys Leu Arg Val Thr Glu Gly 
        115                 120                 125             
Trp Asp Glu Asp Gly His His Ser Glu Glu Ser Leu His Tyr Glu Gly 
    130                 135                 140                 
Arg Ala Val Asp Ile Thr Thr Ser Asp Arg Asp Arg Ser Lys Tyr Gly 
145                 150                 155                 160 
Met Leu Ala Arg Leu Ala Val Glu Ala Gly Phe Asp Trp Val Tyr Tyr 
                165                 170                 175     
Glu Ser Lys Ala His Ile His Cys Ser Val Lys Ala Glu Asn Ser Val 
            180                 185                 190         
Ala Ala Lys Ser Gly Gly 
        195             
<210>  2
<211>  678
<212>  PRT
<213>  ˹
<220>  
<223>  Arl13b-tagRFPT
<400>  2
Met Phe Ser Leu Met Ala Ser Cys Cys Gly Trp Phe Lys Arg Trp Arg 
1               5                   10                  15      
Glu Pro Val Arg Lys Val Thr Leu Leu Met Val Gly Leu Asp Asn Ala 
            20                  25                  30          
Gly Lys Thr Ala Thr Ala Lys Gly Ile Gln Gly Glu Tyr Pro Glu Asp 
        35                  40                  45              
Val Ala Pro Thr Val Gly Phe Ser Lys Ile Asn Leu Arg Gln Gly Lys 
    50                  55                  60                  
Phe Glu Val Thr Ile Phe Asp Leu Gly Gly Gly Ile Arg Ile Arg Gly 
65                  70                  75                  80  
Ile Trp Lys Asn Tyr Tyr Ala Glu Ser Tyr Gly Val Ile Phe Val Val 
                85                  90                  95      
Asp Ser Ser Asp Glu Glu Arg Met Glu Glu Thr Lys Glu Ala Met Ser 
            100                 105                 110         
Glu Met Leu Arg His Pro Arg Ile Ser Gly Lys Pro Ile Leu Val Leu 
        115                 120                 125             
Ala Asn Lys Gln Asp Lys Glu Gly Ala Leu Gly Glu Ala Asp Val Ile 
    130                 135                 140                 
Glu Cys Leu Ser Leu Glu Lys Leu Val Asn Glu His Lys Cys Leu Cys 
145                 150                 155                 160 

Gln Ile Glu Pro Cys Ser Ala Ile Ser Gly Tyr Gly Lys Lys Ile Asp 
                165                 170                 175     
Lys Ser Ile Lys Lys Gly Leu Tyr Trp Leu Leu His Val Ile Ala Arg 
            180                 185                 190         
Asp Phe Asp Ala Leu Asn Glu Arg Ile Gln Lys Glu Thr Thr Glu Gln 
        195                 200                 205             
Arg Ala Leu Glu Glu Gln Glu Lys Gln Glu Arg Ala Glu Arg Val Arg 
    210                 215                 220                 
Lys Leu Arg Glu Glu Arg Lys Gln Asn Glu Gln Glu Gln Ala Glu Leu 
225                 230                 235                 240 
Asp Gly Thr Ser Gly Leu Ala Glu Leu Asp Pro Glu Pro Thr Asn Pro 
                245                 250                 255     
Phe Gln Pro Ile Ala Ser Val Ile Ile Glu Asn Glu Gly Lys Leu Glu 
            260                 265                 270         
Arg Glu Lys Lys Asn Gln Lys Met Glu Lys Asp Ser Asp Gly Cys His 
        275                 280                 285             
Leu Lys His Lys Met Glu His Glu Gln Ile Glu Thr Gln Gly Gln Val 
    290                 295                 300                 
Asn His Asn Gly Gln Lys Asn Asn Glu Phe Gly Leu Val Glu Asn Tyr 
305                 310                 315                 320 
Lys Glu Ala Leu Thr Gln Gln Leu Lys Asn Glu Asp Glu Thr Asp Arg 
                325                 330                 335     
Pro Ser Leu Glu Ser Ala Asn Gly Lys Lys Lys Thr Lys Lys Leu Arg 
            340                 345                 350         
Met Lys Arg Asn His Arg Val Glu Pro Leu Asn Ile Asp Asp Cys Ala 
        355                 360                 365             
Pro Glu Ser Pro Thr Pro Pro Pro Pro Pro Pro Pro Val Gly Trp Gly 
    370                 375                 380                 
Thr Pro Lys Val Thr Arg Leu Pro Lys Leu Glu Pro Leu Gly Glu Thr 
385                 390                 395                 400 
His His Asn Asp Phe Tyr Arg Lys Pro Leu Pro Pro Leu Ala Val Pro 
                405                 410                 415     
Gln Arg Pro Asn Ser Asp Ala His Asp Val Ile Ser Leu Ile Asn Gly 
            420                 425                 430         
Ser Gly Met Val Ser Lys Gly Glu Glu Leu Ile Lys Glu Asn Met His 
        435                 440                 445             
Met Lys Leu Tyr Met Glu Gly Thr Val Asn Asn His His Phe Lys Cys 
    450                 455                 460                 
Thr Ser Glu Gly Glu Gly Lys Pro Tyr Glu Gly Thr Gln Thr Met Arg 
465                 470                 475                 480 
Ile Lys Val Val Glu Gly Gly Pro Leu Pro Phe Ala Phe Asp Ile Leu 
                485                 490                 495     
Ala Thr Ser Phe Met Tyr Gly Ser Arg Thr Phe Ile Asn His Thr Gln 
            500                 505                 510         
Gly Ile Pro Asp Phe Phe Lys Gln Ser Phe Pro Glu Gly Phe Thr Trp 
        515                 520                 525             
Glu Arg Val Thr Thr Tyr Glu Asp Gly Gly Val Leu Thr Ala Thr Gln 
    530                 535                 540                 
Asp Thr Ser Leu Gln Asp Gly Cys Leu Ile Tyr Asn Val Lys Ile Arg 
545                 550                 555                 560 
Gly Val Asn Phe Pro Ser Asn Gly Pro Val Met Gln Lys Lys Thr Leu 
                565                 570                 575     
Gly Trp Glu Ala Asn Thr Glu Met Leu Tyr Pro Ala Asp Gly Gly Leu 
            580                 585                 590         
Glu Gly Arg Thr Asp Met Ala Leu Lys Leu Val Gly Gly Gly His Leu 
        595                 600                 605             
Ile Cys Asn Phe Lys Thr Thr Tyr Arg Ser Lys Lys Pro Ala Lys Asn 
    610                 615                 620                 
Leu Lys Met Pro Gly Val Tyr Tyr Val Asp His Arg Leu Glu Arg Ile 
625                 630                 635                 640 
Lys Glu Ala Asp Lys Glu Thr Tyr Val Glu Gln His Glu Val Ala Val 
                645                 650                 655     
Ala Arg Tyr Cys Asp Leu Pro Ser Lys Leu Gly His Lys Leu Asn Gly 
            660                 665                 670         

Met Asp Glu Leu Tyr Lys 
        675             
<210>  3
<211>  1866
<212>  PRT
<213>  ˹
<220>  
<223>  EGFP-Gli2-3Flag-3Myc
<400>  3
Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu 
1               5                   10                  15      
Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly 
            20                  25                  30          
Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile 
        35                  40                  45              
Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr 
    50                  55                  60                  
Leu Thr Tyr Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys 
65                  70                  75                  80  
Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu 
                85                  90                  95      
Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu 
            100                 105                 110         
Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly 
        115                 120                 125             
Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr 
    130                 135                 140                 
Asn Tyr Asn Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn 
145                 150                 155                 160 
Gly Ile Lys Val Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser 
                165                 170                 175     
Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly 
            180                 185                 190         
Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Ala Leu 
        195                 200                 205             
Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe 
    210                 215                 220                 
Val Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys Gly 
225                 230                 235                 240 
Ser Gly Ser Gly Ser Gly Leu Ile Asn Glu Thr Ser Ala Pro Ala Pro 
                245                 250                 255     
Ala Leu Glu Lys Lys Glu Ala Lys Ser Gly Leu Leu Glu Asp Ser Ser 
            260                 265                 270         
Phe Pro Asp Pro Gly Lys Lys Ala Cys Pro Leu Ala Val Ala Ala Ala 
        275                 280                 285             
Val Ala Ala His Gly Val Pro Gln Gln Leu Leu Pro Ala Phe His Ala 
    290                 295                 300                 
Pro Leu Pro Ile Asp Met Arg His Gln Glu Gly Arg Tyr His Tyr Asp 
305                 310                 315                 320 
Pro His Ser Val His Ser Val His Gly Pro Pro Thr Leu Ser Gly Ser 
                325                 330                 335     
Pro Val Ile Ser Asp Ile Ser Leu Ile Arg Leu Ser Pro His Pro Ala 
            340                 345                 350         
Gly Pro Gly Glu Ser Pro Phe Ser Ala His His Pro Tyr Val Asn Pro 
        355                 360                 365             
His Met Glu His Tyr Leu Arg Ser Val His Ser Ser Pro Thr Leu Ser 
    370                 375                 380                 
Met Ile Ser Ala Ala Arg Gly Leu Ser Pro Ala Asp Val Ala His Glu 
385                 390                 395                 400 
His Leu Lys Glu Arg Gly Leu Phe Ser Leu Ala Ala Pro Gly Thr Asn 
                405                 410                 415     
Pro Ser Asp Tyr Tyr His Gln Met Thr Leu Met Ala Ser His Pro Thr 
            420                 425                 430         
Pro Tyr Gly Asp Leu Leu Met Gln Ser Gly Gly Ala Ala Ser Ala Pro 
        435                 440                 445             
His Leu His Asp Tyr Leu Asn Pro Val Asp Ala Ser Arg Phe Ser Ser 
    450                 455                 460                 
Pro Arg Val Thr Pro Arg Leu Ser Arg Lys Arg Ala Leu Ser Ile Ser 
465                 470                 475                 480 
Pro Leu Ser Asp Ala Ser Leu Asp Leu Gln Arg Met Ile Arg Thr Ser 
                485                 490                 495     
Pro Asn Ser Leu Val Ala Tyr Ile Asn Asn Ser Arg Ser Ser Ser Ala 
            500                 505                 510         
Ala Ser Gly Ser Tyr Gly His Leu Ser Ala Gly Ala Leu Ser Pro Ala 
        515                 520                 525             
Phe Thr Phe Pro His Pro Ile Asn Pro Val Ala Tyr Gln Gln Ile Leu 
    530                 535                 540                 
Ser Gln Gln Arg Gly Leu Gly Ser Ala Phe Gly His Thr Pro Pro Leu 
545                 550                 555                 560 
Ile Gln Pro Ser Pro Thr Phe Leu Ala Gln Gln Pro Met Thr Leu Thr 
                565                 570                 575     
Ser Ile Ser Thr Met Pro Thr Gln Leu Ser Ser Ser Ser Ser Asn Cys 
            580                 585                 590         
Leu Asn Asp Ala Asn Gln Asn Lys Gln Asn Ser Glu Ser Ala Val Ser 
        595                 600                 605             
Ser Thr Val Asn Pro Ile Thr Ile His Lys Arg Ser Lys Val Lys Thr 
    610                 615                 620                 
Glu Ala Glu Gly Leu Arg Pro Ala Ser Pro Leu Gly Leu Thr Gln Glu 
625                 630                 635                 640 
Gln Leu Ala Asp Leu Lys Glu Asp Leu Asp Arg Asp Asp Cys Lys Gln 
                645                 650                 655     
Glu Ala Glu Val Val Ile Tyr Glu Thr Asn Cys His Trp Ala Asp Cys 
            660                 665                 670         
Thr Lys Glu Tyr Asp Thr Gln Glu Gln Leu Val His His Ile Asn Asn 
        675                 680                 685             
Glu His Ile His Gly Glu Lys Lys Glu Phe Val Cys Arg Trp Gln Ala 
    690                 695                 700                 
Cys Thr Arg Glu Gln Lys Pro Phe Lys Ala Gln Tyr Met Leu Val Val 
705                 710                 715                 720 
His Met Arg Arg His Thr Gly Glu Lys Pro His Lys Cys Thr Phe Glu 
                725                 730                 735     
Gly Cys Ser Lys Ala Tyr Ser Arg Leu Glu Asn Leu Lys Thr His Leu 
            740                 745                 750         
Arg Ser His Thr Gly Glu Lys Pro Tyr Val Cys Glu His Glu Gly Cys 
        755                 760                 765             
Asn Lys Ala Phe Ser Asn Ala Ser Asp Arg Ala Lys His Gln Asn Arg 
    770                 775                 780                 
Thr His Ser Asn Glu Lys Pro Tyr Ile Cys Lys Ile Pro Gly Cys Thr 
785                 790                 795                 800 
Lys Arg Tyr Thr Asp Pro Ser Ser Leu Arg Lys His Val Lys Thr Val 
                805                 810                 815     
His Gly Pro Asp Ala His Val Thr Lys Lys Gln Arg Asn Asp Val His 
            820                 825                 830         
Val Arg Ala Pro Leu Leu Lys Glu Asn Gly Asp Asn Glu Ala Ser Ala 
        835                 840                 845             
Glu Pro Gly Gly Arg Gly Pro Glu Glu Ser Val Glu Ala Ser Ser Thr 
    850                 855                 860                 
Ser His Thr Val Glu Asp Cys Leu His Ile Lys Ala Ile Lys Thr Glu 
865                 870                 875                 880 
Ser Ser Gly Leu Cys Gln Ser Ser Pro Gly Ala Gln Ser Ser Cys Ser 
                885                 890                 895     
Ser Glu Pro Ser Pro Leu Gly Ser Ala Pro Asn Asn Asp Ser Gly Met 
            900                 905                 910         
Glu Met Pro Gly Thr Gly Pro Gly Ser Leu Gly Asp Leu Thr Ala Leu 
        915                 920                 925             
Ala Asp Thr Cys Pro Gly Ala Asp Thr Ser Ala Leu Ala Ala Pro Ser 
    930                 935                 940                 
Thr Gly Gly Leu Gln Leu Arg Lys His Met Ser Thr Val His Arg Phe 
945                 950                 955                 960 
Glu Gln Leu Lys Arg Glu Lys Leu Lys Ser Leu Lys Asp Ser Cys Ser 
                965                 970                 975     
Trp Ala Gly Pro Ala Pro His Thr Arg Asn Thr Lys Leu Pro Pro Leu 
            980                 985                 990         
Pro Val Asn Gly Ser Val Leu Glu  Asn Phe Asn Asn Thr  Gly Gly Gly 
        995                 1000                 1005             
Gly Pro  Ala Gly Leu Leu Pro  Ser Gln Arg Leu Pro  Glu Leu Thr 
    1010                 1015                 1020             
Glu Val  Thr Met Leu Ser Gln  Leu Gln Glu Arg Arg  Asp Ser Ser 
    1025                 1030                 1035             
Thr Ser  Thr Met Ser Ser Ala  Tyr Thr Val Ser Arg  Arg Ser Ser 
    1040                 1045                 1050             
Gly Ile  Ser Pro Tyr Phe Ser  Ser Arg Arg Ser Ser  Glu Ala Ser 
    1055                 1060                 1065             
Pro Leu  Gly Gly Leu Arg Pro  His Asn Ala Ser Ser  Ala Asp Ser 
    1070                 1075                 1080             
Tyr Asp  Pro Ile Ser Thr Asp  Ala Ser Arg Arg Ser  Ser Glu Ala 
    1085                 1090                 1095             
Ser Gln  Cys Ser Gly Gly Gly  Pro Gly Leu Leu Asn  Leu Thr Pro 
    1100                 1105                 1110             
Ala Gln  Gln Tyr Asn Leu Arg  Ala Lys Tyr Ala Ala  Ala Thr Gly 
    1115                 1120                 1125             
Gly Pro  Pro Pro Thr Pro Leu  Pro Gly Leu Asp Arg  Val Ser Leu 
    1130                 1135                 1140             
Arg Thr  Arg Leu Ala Leu Leu  Asp Ala Pro Glu Arg  Ala Leu Pro 
    1145                 1150                 1155             
Gly Ala  Cys Pro His Pro Leu  Gly Pro Arg Arg Gly  Ser Asp Gly 
    1160                 1165                 1170             
Pro Thr  Tyr Ser His Gly His  Gly His Gly Tyr Ala  Gly Ala Ala 
    1175                 1180                 1185             
Pro Ala  Phe Pro His Glu Gly  Pro Asn Ser Ser Thr  Arg Arg Ala 
    1190                 1195                 1200             
Ser Asp  Pro Val Arg Arg Pro  Asp Pro Leu Ile Leu  Pro Arg Val 
    1205                 1210                 1215             
Gln Arg  Phe His Ser Thr His  Asn Met Asn Pro Gly  Ser Leu Pro 
    1220                 1225                 1230             
Pro Cys  Ala Asp Arg Arg Gly  Leu His Val Gln Ser  His Pro Ser 
    1235                 1240                 1245             
Val Asp  Ser Asn Leu Thr Arg  Asn Ala Tyr Ser Pro  Arg Pro Pro 
    1250                 1255                 1260             
Ser Ile  Asn Glu Asn Val Val  Met Glu Ala Val Ala  Ala Gly Val 
    1265                 1270                 1275             
Asp Gly  Pro Gly Leu Glu Cys  Asp Leu Gly Leu Val  Glu Asp Glu 
    1280                 1285                 1290             
Leu Val  Leu Pro Asp Asp Val  Val Gln Tyr Ile Lys  Ala His Thr 
    1295                 1300                 1305             
Gly Gly  Thr Leu Asp Asp Gly  Ile Arg Gln Gly Tyr  Pro Thr Glu 
    1310                 1315                 1320             
Gly Thr  Gly Phe Pro Glu Asn  Ser Lys Leu Pro Ser  Pro Gly Leu 
    1325                 1330                 1335             
Gln Gly  His Arg Arg Leu Ala  Ala Ala Asp Ser Asn  Met Gly Pro 
    1340                 1345                 1350             
Ser Ala  Pro Gly Leu Gly Gly  Cys Gln Leu Ser Tyr  Ser Pro Ser 
    1355                 1360                 1365             
Ser Asn  Leu Asn Lys Ser Asn  Met Pro Val Gln Trp  Asn Glu Val 
    1370                 1375                 1380             
Ser Ser  Gly Thr Val Asp Ala  Leu Pro Thr Gln Val  Lys Pro Pro 
    1385                 1390                 1395             
Pro Phe  Pro His Ser Asn Leu  Ala Val Val Gln Gln  Lys Pro Ala 
    1400                 1405                 1410             
Phe Gly  Gln Tyr Pro Gly Tyr  Asn Pro Gln Ser Val  Gln Ser Ser 
    1415                 1420                 1425             
Ser Gly  Gly Leu Asp Ser Thr  Gln Pro His Leu Gln  Leu Arg Gly 
    1430                 1435                 1440             
Ala Pro  Ser Ala Ser Arg Gly  Ser Tyr Thr Gln Gln  Pro Arg Gln 
    1445                 1450                 1455             
Pro Ala  Ala Gly Ser Gln Cys  Leu Gly Met Ser Ala  Ala Met Ser 
    1460                 1465                 1470             
Pro Gln  Ala Ser Tyr Ser Gln  Ala His Pro Gln Leu  Ser Pro Asn 
    1475                 1480                 1485             
Ile Val  Ser Gly Ser Leu Asn  Gln Phe Ser Pro Ser  Cys Ser Asn 
    1490                 1495                 1500             
Met Ala  Ala Lys Pro Ser His  Leu Gly Leu Pro Gln  Gln Met Glu 
    1505                 1510                 1515             
Val Val  Pro Asn Ala Thr Ile  Met Asn Gly His Gln  Arg Glu His 
    1520                 1525                 1530             
Gly Val  Pro Asn Ser Ser Leu  Ala Ala Val Ser Gln  Pro His Pro 
    1535                 1540                 1545             
Val Leu  Ser Tyr Pro Gln Gln  Asp Ser Tyr Gln Gln  Gly Ser Asn 
    1550                 1555                 1560             
Leu Leu  Ser Ser His Gln Pro  Gly Phe Met Glu Ser  Gln Gln Asn 
    1565                 1570                 1575             
Ala Gly  Phe Gly Leu Met Gln  Pro Arg Pro Pro Leu  Glu Pro Asn 
    1580                 1585                 1590             
Thr Ala  Ser Arg His Arg Gly  Val Arg Ser Gly Gln  Gln Gln Leu 
    1595                 1600                 1605             
Tyr Ala  Arg Thr Thr Gly Gln  Ala Met Val Thr Ser  Ala Asn Gln 
    1610                 1615                 1620             
Glu Thr  Ala Glu Ala Met Pro  Lys Gly Pro Ala Gly  Thr Met Val 
    1625                 1630                 1635             
Ser Leu  Ala Pro Gln Pro Ser  Gln Asp Thr Gly Arg  Ala Gln Asp 
    1640                 1645                 1650             
Gln Asn  Thr Leu Tyr Tyr Tyr  Gly Gln Ile His Met  Tyr Glu Gln 
    1655                 1660                 1665             
Asn Gly  Gly Cys Pro Ala Val  Gln Pro Gln Pro Pro  Gln Pro Gln 
    1670                 1675                 1680             
Ala Cys  Ser Asp Ser Ile Gln  Pro Glu Pro Leu Pro  Ser Pro Gly 
    1685                 1690                 1695             
Val Asn  Gln Val Ser Ser Thr  Val Asp Ser Gln Leu  Leu Glu Pro 
    1700                 1705                 1710             
Pro Gln  Ile Asp Phe Asp Ala  Ile Met Asp Asp Gly  Asp His Ser 
    1715                 1720                 1725             
Ser Leu  Phe Ser Gly Ala Leu  Ser Pro Thr Leu Leu  His Asn Leu 
    1730                 1735                 1740             
Ser Gln  Asn Ser Ser Arg Leu  Thr Thr Pro Arg Asn  Ser Leu Thr 
    1745                 1750                 1755             
Leu Pro  Ser Ile Pro Ala Gly  Ile Ser Asn Met Ala  Val Gly Asp 
    1760                 1765                 1770             
Met Ser  Ser Met Leu Thr Ser  Leu Ala Glu Glu Ser  Lys Phe Leu 
    1775                 1780                 1785             
Asn Met  Met Thr Gly Ser Gly  Ser Gly Ser Gly Ser  Gly Asp Tyr 
    1790                 1795                 1800             
Lys Asp  His Asp Gly Asp Tyr  Lys Asp His Asp Ile  Asp Tyr Lys 
    1805                 1810                 1815             
Asp Asp  Asp Asp Lys Gly Ser  Gly Ser Gly Ser Gly  Glu Gln Lys 
    1820                 1825                 1830             
Leu Ile  Ser Glu Glu Asp Leu  Asn Gly Glu Gln Lys  Leu Ile Ser 
    1835                 1840                 1845             
Glu Glu  Asp Leu Asn Gly Glu  Gln Lys Leu Ile Ser  Glu Glu Asp 
    1850                 1855                 1860             
Leu Asn  His 
    1865     
<210>  4
<211>  550
<212>  PRT
<213>  ˹
<220>  
<223>  өөø
<400>  4
Met Glu Asp Ala Lys Asn Ile Lys Lys Gly Pro Ala Pro Phe Tyr Pro 
1               5                   10                  15      
Leu Glu Asp Gly Thr Ala Gly Glu Gln Leu His Lys Ala Met Lys Arg 
            20                  25                  30          
Tyr Ala Leu Val Pro Gly Thr Ile Ala Phe Thr Asp Ala His Ile Glu 
        35                  40                  45              
Val Asp Ile Thr Tyr Ala Glu Tyr Phe Glu Met Ser Val Arg Leu Ala 
    50                  55                  60                  
Glu Ala Met Lys Arg Tyr Gly Leu Asn Thr Asn His Arg Ile Val Val 
65                  70                  75                  80  
Cys Ser Glu Asn Ser Leu Gln Phe Phe Met Pro Val Leu Gly Ala Leu 
                85                  90                  95      
Phe Ile Gly Val Ala Val Ala Pro Ala Asn Asp Ile Tyr Asn Glu Arg 
            100                 105                 110         
Glu Leu Leu Asn Ser Met Gly Ile Ser Gln Pro Thr Val Val Phe Val 
        115                 120                 125             
Ser Lys Lys Gly Leu Gln Lys Ile Leu Asn Val Gln Lys Lys Leu Pro 
    130                 135                 140                 
Ile Ile Gln Lys Ile Ile Ile Met Asp Ser Lys Thr Asp Tyr Gln Gly 
145                 150                 155                 160 
Phe Gln Ser Met Tyr Thr Phe Val Thr Ser His Leu Pro Pro Gly Phe 
                165                 170                 175     
Asn Glu Tyr Asp Phe Val Pro Glu Ser Phe Asp Arg Asp Lys Thr Ile 
            180                 185                 190         
Ala Leu Ile Met Asn Ser Ser Gly Ser Thr Gly Leu Pro Lys Gly Val 
        195                 200                 205             
Ala Leu Pro His Arg Thr Ala Cys Val Arg Phe Ser His Ala Arg Asp 
    210                 215                 220                 
Pro Ile Phe Gly Asn Gln Ile Ile Pro Asp Thr Ala Ile Leu Ser Val 
225                 230                 235                 240 
Val Pro Phe His His Gly Phe Gly Met Phe Thr Thr Leu Gly Tyr Leu 
                245                 250                 255     
Ile Cys Gly Phe Arg Val Val Leu Met Tyr Arg Phe Glu Glu Glu Leu 
            260                 265                 270         
Phe Leu Arg Ser Leu Gln Asp Tyr Lys Ile Gln Ser Ala Leu Leu Val 
        275                 280                 285             
Pro Thr Leu Phe Ser Phe Phe Ala Lys Ser Thr Leu Ile Asp Lys Tyr 
    290                 295                 300                 
Asp Leu Ser Asn Leu His Glu Ile Ala Ser Gly Gly Ala Pro Leu Ser 
305                 310                 315                 320 
Lys Glu Val Gly Glu Ala Val Ala Lys Arg Phe His Leu Pro Gly Ile 
                325                 330                 335     
Arg Gln Gly Tyr Gly Leu Thr Glu Thr Thr Ser Ala Ile Leu Ile Thr 
            340                 345                 350         
Pro Glu Gly Asp Asp Lys Pro Gly Ala Val Gly Lys Val Val Pro Phe 
        355                 360                 365             
Phe Glu Ala Lys Val Val Asp Leu Asp Thr Gly Lys Thr Leu Gly Val 
    370                 375                 380                 
Asn Gln Arg Gly Glu Leu Cys Val Arg Gly Pro Met Ile Met Ser Gly 
385                 390                 395                 400 
Tyr Val Asn Asn Pro Glu Ala Thr Asn Ala Leu Ile Asp Lys Asp Gly 
                405                 410                 415     
Trp Leu His Ser Gly Asp Ile Ala Tyr Trp Asp Glu Asp Glu His Phe 
            420                 425                 430         
Phe Ile Val Asp Arg Leu Lys Ser Leu Ile Lys Tyr Lys Gly Tyr Gln 
        435                 440                 445             
Val Ala Pro Ala Glu Leu Glu Ser Ile Leu Leu Gln His Pro Asn Ile 
    450                 455                 460                 
Phe Asp Ala Gly Val Ala Gly Leu Pro Asp Asp Asp Ala Gly Glu Leu 
465                 470                 475                 480 
Pro Ala Ala Val Val Val Leu Glu His Gly Lys Thr Met Thr Glu Lys 
                485                 490                 495     
Glu Ile Val Asp Tyr Val Ala Ser Gln Val Thr Thr Ala Lys Lys Leu 
            500                 505                 510         
Arg Gly Gly Val Val Phe Val Asp Glu Val Pro Lys Gly Leu Thr Gly 
        515                 520                 525             
Lys Leu Asp Ala Arg Lys Ile Arg Glu Ile Leu Ile Lys Ala Lys Lys 
    530                 535                 540                 
Gly Gly Lys Ile Ala Val 
545                 550 
<210>  5
<211>  311
<212>  PRT
<213>  ˹
<220>  
<223>  өø
<400>  5
Met Thr Ser Lys Val Tyr Asp Pro Glu Gln Arg Lys Arg Met Ile Thr 
1               5                   10                  15      
Gly Pro Gln Trp Trp Ala Arg Cys Lys Gln Met Asn Val Leu Asp Ser 
            20                  25                  30          
Phe Ile Asn Tyr Tyr Asp Ser Glu Lys His Ala Glu Asn Ala Val Ile 
        35                  40                  45              
Phe Leu His Gly Asn Ala Ala Ser Ser Tyr Leu Trp Arg His Val Val 
    50                  55                  60                  
Pro His Ile Glu Pro Val Ala Arg Cys Ile Ile Pro Asp Leu Ile Gly 
65                  70                  75                  80  
Met Gly Lys Ser Gly Lys Ser Gly Asn Gly Ser Tyr Arg Leu Leu Asp 
                85                  90                  95      
His Tyr Lys Tyr Leu Thr Ala Trp Phe Glu Leu Leu Asn Leu Pro Lys 
            100                 105                 110         
Lys Ile Ile Phe Val Gly His Asp Trp Gly Ala Cys Leu Ala Phe His 
        115                 120                 125             
Tyr Ser Tyr Glu His Gln Asp Lys Ile Lys Ala Ile Val His Ala Glu 
    130                 135                 140                 
Ser Val Val Asp Val Ile Glu Ser Trp Asp Glu Trp Pro Asp Ile Glu 
145                 150                 155                 160 
Glu Asp Ile Ala Leu Ile Lys Ser Glu Glu Gly Glu Lys Met Val Leu 
                165                 170                 175     
Glu Asn Asn Phe Phe Val Glu Thr Met Leu Pro Ser Lys Ile Met Arg 
            180                 185                 190         
Lys Leu Glu Pro Glu Glu Phe Ala Ala Tyr Leu Glu Pro Phe Lys Glu 
        195                 200                 205             
Lys Gly Glu Val Arg Arg Pro Thr Leu Ser Trp Pro Arg Glu Ile Pro 
    210                 215                 220                 
Leu Val Lys Gly Gly Lys Pro Asp Val Val Gln Ile Val Arg Asn Tyr 
225                 230                 235                 240 
Asn Ala Tyr Leu Arg Ala Ser Asp Asp Leu Pro Lys Met Phe Ile Glu 
                245                 250                 255     
Ser Asp Pro Gly Phe Phe Ser Asn Ala Ile Val Glu Gly Ala Lys Lys 
            260                 265                 270         
Phe Pro Asn Thr Glu Phe Val Lys Val Lys Gly Leu His Phe Ser Gln 
        275                 280                 285             
Glu Asp Ala Pro Asp Glu Met Gly Lys Tyr Ile Lys Ser Phe Val Glu 
    290                 295                 300                 
Arg Val Leu Lys Asn Glu Gln 
305                 310     
<210>  6
<211>  1544
<212>  PRT
<213>  ˹
<220>  
<223>  СGLI2
<400>  6
Met Glu Thr Ser Ala Pro Ala Pro Ala Leu Glu Lys Lys Glu Ala Lys 
1               5                   10                  15      
Ser Gly Leu Leu Glu Asp Ser Ser Phe Pro Asp Pro Gly Lys Lys Ala 
            20                  25                  30          
Cys Pro Leu Ala Val Ala Ala Ala Val Ala Ala His Gly Val Pro Gln 
        35                  40                  45              
Gln Leu Leu Pro Ala Phe His Ala Pro Leu Pro Ile Asp Met Arg His 
    50                  55                  60                  
Gln Glu Gly Arg Tyr His Tyr Asp Pro His Ser Val His Ser Val His 
65                  70                  75                  80  
Gly Pro Pro Thr Leu Ser Gly Ser Pro Val Ile Ser Asp Ile Ser Leu 
                85                  90                  95      
Ile Arg Leu Ser Pro His Pro Ala Gly Pro Gly Glu Ser Pro Phe Ser 
            100                 105                 110         
Ala His His Pro Tyr Val Asn Pro His Met Glu His Tyr Leu Arg Ser 
        115                 120                 125             
Val His Ser Ser Pro Thr Leu Ser Met Ile Ser Ala Ala Arg Gly Leu 
    130                 135                 140                 
Ser Pro Ala Asp Val Ala His Glu His Leu Lys Glu Arg Gly Leu Phe 
145                 150                 155                 160 
Ser Leu Ala Ala Pro Gly Thr Asn Pro Ser Asp Tyr Tyr His Gln Met 
                165                 170                 175     
Thr Leu Met Ala Ser His Pro Thr Pro Tyr Gly Asp Leu Leu Met Gln 
            180                 185                 190         
Ser Gly Gly Ala Ala Ser Ala Pro His Leu His Asp Tyr Leu Asn Pro 
        195                 200                 205             
Val Asp Ala Ser Arg Phe Ser Ser Pro Arg Val Thr Pro Arg Leu Ser 
    210                 215                 220                 
Arg Lys Arg Ala Leu Ser Ile Ser Pro Leu Ser Asp Ala Ser Leu Asp 
225                 230                 235                 240 
Leu Gln Arg Met Ile Arg Thr Ser Pro Asn Ser Leu Val Ala Tyr Ile 
                245                 250                 255     
Asn Asn Ser Arg Ser Ser Ser Ala Ala Ser Gly Ser Tyr Gly His Leu 
            260                 265                 270         
Ser Ala Gly Ala Leu Ser Pro Ala Phe Thr Phe Pro His Pro Ile Asn 
        275                 280                 285             
Pro Val Ala Tyr Gln Gln Ile Leu Ser Gln Gln Arg Gly Leu Gly Ser 
    290                 295                 300                 
Ala Phe Gly His Thr Pro Pro Leu Ile Gln Pro Ser Pro Thr Phe Leu 
305                 310                 315                 320 
Ala Gln Gln Pro Met Thr Leu Thr Ser Ile Ser Thr Met Pro Thr Gln 
                325                 330                 335     
Leu Ser Ser Ser Ser Ser Asn Cys Leu Asn Asp Ala Asn Gln Asn Lys 
            340                 345                 350         
Gln Asn Ser Glu Ser Ala Val Ser Ser Thr Val Asn Pro Ile Thr Ile 
        355                 360                 365             
His Lys Arg Ser Lys Val Lys Thr Glu Ala Glu Gly Leu Arg Pro Ala 
    370                 375                 380                 
Ser Pro Leu Gly Leu Thr Gln Glu Gln Leu Ala Asp Leu Lys Glu Asp 
385                 390                 395                 400 
Leu Asp Arg Asp Asp Cys Lys Gln Glu Ala Glu Val Val Ile Tyr Glu 
                405                 410                 415     
Thr Asn Cys His Trp Ala Asp Cys Thr Lys Glu Tyr Asp Thr Gln Glu 
            420                 425                 430         
Gln Leu Val His His Ile Asn Asn Glu His Ile His Gly Glu Lys Lys 
        435                 440                 445             
Glu Phe Val Cys Arg Trp Gln Ala Cys Thr Arg Glu Gln Lys Pro Phe 
    450                 455                 460                 
Lys Ala Gln Tyr Met Leu Val Val His Met Arg Arg His Thr Gly Glu 
465                 470                 475                 480 
Lys Pro His Lys Cys Thr Phe Glu Gly Cys Ser Lys Ala Tyr Ser Arg 
                485                 490                 495     
Leu Glu Asn Leu Lys Thr His Leu Arg Ser His Thr Gly Glu Lys Pro 
            500                 505                 510         
Tyr Val Cys Glu His Glu Gly Cys Asn Lys Ala Phe Ser Asn Ala Ser 
        515                 520                 525             
Asp Arg Ala Lys His Gln Asn Arg Thr His Ser Asn Glu Lys Pro Tyr 
    530                 535                 540                 
Ile Cys Lys Ile Pro Gly Cys Thr Lys Arg Tyr Thr Asp Pro Ser Ser 
545                 550                 555                 560 
Leu Arg Lys His Val Lys Thr Val His Gly Pro Asp Ala His Val Thr 
                565                 570                 575     
Lys Lys Gln Arg Asn Asp Val His Val Arg Ala Pro Leu Leu Lys Glu 
            580                 585                 590         
Asn Gly Asp Asn Glu Ala Ser Ala Glu Pro Gly Gly Arg Gly Pro Glu 
        595                 600                 605             
Glu Ser Val Glu Ala Ser Ser Thr Ser His Thr Val Glu Asp Cys Leu 
    610                 615                 620                 
His Ile Lys Ala Ile Lys Thr Glu Ser Ser Gly Leu Cys Gln Ser Ser 
625                 630                 635                 640 
Pro Gly Ala Gln Ser Ser Cys Ser Ser Glu Pro Ser Pro Leu Gly Ser 
                645                 650                 655     
Ala Pro Asn Asn Asp Ser Gly Met Glu Met Pro Gly Thr Gly Pro Gly 
            660                 665                 670         
Ser Leu Gly Asp Leu Thr Ala Leu Ala Asp Thr Cys Pro Gly Ala Asp 
        675                 680                 685             
Thr Ser Ala Leu Ala Ala Pro Ser Thr Gly Gly Leu Gln Leu Arg Lys 
    690                 695                 700                 
His Met Ser Thr Val His Arg Phe Glu Gln Leu Lys Arg Glu Lys Leu 
705                 710                 715                 720 
Lys Ser Leu Lys Asp Ser Cys Ser Trp Ala Gly Pro Ala Pro His Thr 
                725                 730                 735     
Arg Asn Thr Lys Leu Pro Pro Leu Pro Val Asn Gly Ser Val Leu Glu 
            740                 745                 750         
Asn Phe Asn Asn Thr Gly Gly Gly Gly Pro Ala Gly Leu Leu Pro Ser 
        755                 760                 765             
Gln Arg Leu Pro Glu Leu Thr Glu Val Thr Met Leu Ser Gln Leu Gln 
    770                 775                 780                 
Glu Arg Arg Asp Ser Ser Thr Ser Thr Met Ser Ser Ala Tyr Thr Val 
785                 790                 795                 800 
Ser Arg Arg Ser Ser Gly Ile Ser Pro Tyr Phe Ser Ser Arg Arg Ser 
                805                 810                 815     
Ser Glu Ala Ser Pro Leu Gly Gly Leu Arg Pro His Asn Ala Ser Ser 
            820                 825                 830         
Ala Asp Ser Tyr Asp Pro Ile Ser Thr Asp Ala Ser Arg Arg Ser Ser 
        835                 840                 845             
Glu Ala Ser Gln Cys Ser Gly Gly Gly Pro Gly Leu Leu Asn Leu Thr 
    850                 855                 860                 
Pro Ala Gln Gln Tyr Asn Leu Arg Ala Lys Tyr Ala Ala Ala Thr Gly 
865                 870                 875                 880 
Gly Pro Pro Pro Thr Pro Leu Pro Gly Leu Asp Arg Val Ser Leu Arg 
                885                 890                 895     
Thr Arg Leu Ala Leu Leu Asp Ala Pro Glu Arg Ala Leu Pro Gly Ala 
            900                 905                 910         
Cys Pro His Pro Leu Gly Pro Arg Arg Gly Ser Asp Gly Pro Thr Tyr 
        915                 920                 925             
Ser His Gly His Gly His Gly Tyr Ala Gly Ala Ala Pro Ala Phe Pro 
    930                 935                 940                 
His Glu Gly Pro Asn Ser Ser Thr Arg Arg Ala Ser Asp Pro Val Arg 
945                 950                 955                 960 
Arg Pro Asp Pro Leu Ile Leu Pro Arg Val Gln Arg Phe His Ser Thr 
                965                 970                 975     
His Asn Met Asn Pro Gly Ser Leu Pro Pro Cys Ala Asp Arg Arg Gly 
            980                 985                 990         
Leu His Val Gln Ser His Pro Ser  Val Asp Ser Asn Leu  Thr Arg Asn 
        995                 1000                 1005             
Ala Tyr  Ser Pro Arg Pro Pro  Ser Ile Asn Glu Asn  Val Val Met 
    1010                 1015                 1020             
Glu Ala  Val Ala Ala Gly Val  Asp Gly Pro Gly Leu  Glu Cys Asp 
    1025                 1030                 1035             
Leu Gly  Leu Val Glu Asp Glu  Leu Val Leu Pro Asp  Asp Val Val 
    1040                 1045                 1050             
Gln Tyr  Ile Lys Ala His Thr  Gly Gly Thr Leu Asp  Asp Gly Ile 
    1055                 1060                 1065             
Arg Gln  Gly Tyr Pro Thr Glu  Gly Thr Gly Phe Pro  Glu Asn Ser 
    1070                 1075                 1080             
Lys Leu  Pro Ser Pro Gly Leu  Gln Gly His Arg Arg  Leu Ala Ala 
    1085                 1090                 1095             
Ala Asp  Ser Asn Met Gly Pro  Ser Ala Pro Gly Leu  Gly Gly Cys 
    1100                 1105                 1110             
Gln Leu  Ser Tyr Ser Pro Ser  Ser Asn Leu Asn Lys  Ser Asn Met 
    1115                 1120                 1125             
Pro Val  Gln Trp Asn Glu Val  Ser Ser Gly Thr Val  Asp Ala Leu 
    1130                 1135                 1140             
Pro Thr  Gln Val Lys Pro Pro  Pro Phe Pro His Ser  Asn Leu Ala 
    1145                 1150                 1155             
Val Val  Gln Gln Lys Pro Ala  Phe Gly Gln Tyr Pro  Gly Tyr Asn 
    1160                 1165                 1170             
Pro Gln  Ser Val Gln Ser Ser  Ser Gly Gly Leu Asp  Ser Thr Gln 
    1175                 1180                 1185             
Pro His  Leu Gln Leu Arg Gly  Ala Pro Ser Ala Ser  Arg Gly Ser 
    1190                 1195                 1200             
Tyr Thr  Gln Gln Pro Arg Gln  Pro Ala Ala Gly Ser  Gln Cys Leu 
    1205                 1210                 1215             
Gly Met  Ser Ala Ala Met Ser  Pro Gln Ala Ser Tyr  Ser Gln Ala 
    1220                 1225                 1230             
His Pro  Gln Leu Ser Pro Asn  Ile Val Ser Gly Ser  Leu Asn Gln 
    1235                 1240                 1245             
Phe Ser  Pro Ser Cys Ser Asn  Met Ala Ala Lys Pro  Ser His Leu 
    1250                 1255                 1260             
Gly Leu  Pro Gln Gln Met Glu  Val Val Pro Asn Ala  Thr Ile Met 
    1265                 1270                 1275             
Asn Gly  His Gln Arg Glu His  Gly Val Pro Asn Ser  Ser Leu Ala 
    1280                 1285                 1290             
Ala Val  Ser Gln Pro His Pro  Val Leu Ser Tyr Pro  Gln Gln Asp 
    1295                 1300                 1305             
Ser Tyr  Gln Gln Gly Ser Asn  Leu Leu Ser Ser His  Gln Pro Gly 
    1310                 1315                 1320             
Phe Met  Glu Ser Gln Gln Asn  Ala Gly Phe Gly Leu  Met Gln Pro 
    1325                 1330                 1335             
Arg Pro  Pro Leu Glu Pro Asn  Thr Ala Ser Arg His  Arg Gly Val 
    1340                 1345                 1350             
Arg Ser  Gly Gln Gln Gln Leu  Tyr Ala Arg Thr Thr  Gly Gln Ala 
    1355                 1360                 1365             
Met Val  Thr Ser Ala Asn Gln  Glu Thr Ala Glu Ala  Met Pro Lys 
    1370                 1375                 1380             
Gly Pro  Ala Gly Thr Met Val  Ser Leu Ala Pro Gln  Pro Ser Gln 
    1385                 1390                 1395             
Asp Thr  Gly Arg Ala Gln Asp  Gln Asn Thr Leu Tyr  Tyr Tyr Gly 
    1400                 1405                 1410             
Gln Ile  His Met Tyr Glu Gln  Asn Gly Gly Cys Pro  Ala Val Gln 
    1415                 1420                 1425             
Pro Gln  Pro Pro Gln Pro Gln  Ala Cys Ser Asp Ser  Ile Gln Pro 
    1430                 1435                 1440             
Glu Pro  Leu Pro Ser Pro Gly  Val Asn Gln Val Ser  Ser Thr Val 
    1445                 1450                 1455             
Asp Ser  Gln Leu Leu Glu Pro  Pro Gln Ile Asp Phe  Asp Ala Ile 
    1460                 1465                 1470             
Met Asp  Asp Gly Asp His Ser  Ser Leu Phe Ser Gly  Ala Leu Ser 
    1475                 1480                 1485             
Pro Thr  Leu Leu His Asn Leu  Ser Gln Asn Ser Ser  Arg Leu Thr 
    1490                 1495                 1500             
Thr Pro  Arg Asn Ser Leu Thr  Leu Pro Ser Ile Pro  Ala Gly Ile 
    1505                 1510                 1515             
Ser Asn  Met Ala Val Gly Asp  Met Ser Ser Met Leu  Thr Ser Leu 
    1520                 1525                 1530             
Ala Glu  Glu Ser Lys Phe Leu  Asn Met Met Thr 
    1535                 1540                 
<210>  7
<211>  1036
<212>  PRT
<213>  ˹
<220>  
<223>  SMO WT-EGFP
<400>  7
Met Ala Ala Ala Arg Pro Ala Arg Gly Pro Glu Leu Pro Leu Leu Gly 
1               5                   10                  15      
Leu Leu Leu Leu Leu Leu Leu Gly Asp Pro Gly Arg Gly Ala Ala Ser 
            20                  25                  30          
Ser Gly Asn Ala Thr Gly Pro Gly Pro Arg Ser Ala Gly Gly Ser Ala 
        35                  40                  45              
Arg Arg Ser Ala Ala Val Thr Gly Pro Pro Pro Pro Leu Ser His Cys 
    50                  55                  60                  
Gly Arg Ala Ala Pro Cys Glu Pro Leu Arg Tyr Asn Val Cys Leu Gly 
65                  70                  75                  80  
Ser Val Leu Pro Tyr Gly Ala Thr Ser Thr Leu Leu Ala Gly Asp Ser 
                85                  90                  95      
Asp Ser Gln Glu Glu Ala His Gly Lys Leu Val Leu Trp Ser Gly Leu 
            100                 105                 110         
Arg Asn Ala Pro Arg Cys Trp Ala Val Ile Gln Pro Leu Leu Cys Ala 
        115                 120                 125             
Val Tyr Met Pro Lys Cys Glu Asn Asp Arg Val Glu Leu Pro Ser Arg 
    130                 135                 140                 
Thr Leu Cys Gln Ala Thr Arg Gly Pro Cys Ala Ile Val Glu Arg Glu 
145                 150                 155                 160 
Arg Gly Trp Pro Asp Phe Leu Arg Cys Thr Pro Asp Arg Phe Pro Glu 
                165                 170                 175     
Gly Cys Thr Asn Glu Val Gln Asn Ile Lys Phe Asn Ser Ser Gly Gln 
            180                 185                 190         
Cys Glu Val Pro Leu Val Arg Thr Asp Asn Pro Lys Ser Trp Tyr Glu 
        195                 200                 205             
Asp Val Glu Gly Cys Gly Ile Gln Cys Gln Asn Pro Leu Phe Thr Glu 
    210                 215                 220                 
Ala Glu His Gln Asp Met His Ser Tyr Ile Ala Ala Phe Gly Ala Val 
225                 230                 235                 240 
Thr Gly Leu Cys Thr Leu Phe Thr Leu Ala Thr Phe Val Ala Asp Trp 
                245                 250                 255     
Arg Asn Ser Asn Arg Tyr Pro Ala Val Ile Leu Phe Tyr Val Asn Ala 
            260                 265                 270         
Cys Phe Phe Val Gly Ser Ile Gly Trp Leu Ala Gln Phe Met Asp Gly 
        275                 280                 285             
Ala Arg Arg Glu Ile Val Cys Arg Ala Asp Gly Thr Met Arg Leu Gly 
    290                 295                 300                 
Glu Pro Thr Ser Asn Glu Thr Leu Ser Cys Val Ile Ile Phe Val Ile 
305                 310                 315                 320 
Val Tyr Tyr Ala Leu Met Ala Gly Val Val Trp Phe Val Val Leu Thr 
                325                 330                 335     
Tyr Ala Trp His Thr Ser Phe Lys Ala Leu Gly Thr Thr Tyr Gln Pro 
            340                 345                 350         
Leu Ser Gly Lys Thr Ser Tyr Phe His Leu Leu Thr Trp Ser Leu Pro 
        355                 360                 365             
Phe Val Leu Thr Val Ala Ile Leu Ala Val Ala Gln Val Asp Gly Asp 
    370                 375                 380                 
Ser Val Ser Gly Ile Cys Phe Val Gly Tyr Lys Asn Tyr Arg Tyr Arg 
385                 390                 395                 400 
Ala Gly Phe Val Leu Ala Pro Ile Gly Leu Val Leu Ile Val Gly Gly 
                405                 410                 415     
Tyr Phe Leu Ile Arg Gly Val Met Thr Leu Phe Ser Ile Lys Ser Asn 
            420                 425                 430         
His Pro Gly Leu Leu Ser Glu Lys Ala Ala Ser Lys Ile Asn Glu Thr 
        435                 440                 445             
Met Leu Arg Leu Gly Ile Phe Gly Phe Leu Ala Phe Gly Phe Val Leu 
    450                 455                 460                 
Ile Thr Phe Ser Cys His Phe Tyr Asp Phe Phe Asn Gln Ala Glu Trp 
465                 470                 475                 480 
Glu Arg Ser Phe Arg Asp Tyr Val Leu Cys Gln Ala Asn Val Thr Ile 
                485                 490                 495     
Gly Leu Pro Thr Lys Gln Pro Ile Pro Asp Cys Glu Ile Lys Asn Arg 
            500                 505                 510         
Pro Ser Leu Leu Val Glu Lys Ile Asn Leu Phe Ala Met Phe Gly Thr 
        515                 520                 525             
Gly Ile Ala Met Ser Thr Trp Val Trp Thr Lys Ala Thr Leu Leu Ile 
    530                 535                 540                 
Trp Arg Arg Thr Trp Cys Arg Leu Thr Gly Gln Ser Asp Asp Glu Pro 
545                 550                 555                 560 
Lys Arg Ile Lys Lys Ser Lys Met Ile Ala Lys Ala Phe Ser Lys Arg 
                565                 570                 575     
His Glu Leu Leu Gln Asn Pro Gly Gln Glu Leu Ser Phe Ser Met His 
            580                 585                 590         
Thr Val Ser His Asp Gly Pro Val Ala Gly Leu Ala Phe Asp Leu Asn 
        595                 600                 605             
Glu Pro Ser Ala Asp Val Ser Ser Ala Trp Ala Gln His Val Thr Lys 
    610                 615                 620                 
Met Val Ala Arg Arg Gly Ala Ile Leu Pro Gln Asp Ile Ser Val Thr 
625                 630                 635                 640 
Pro Val Ala Thr Pro Val Pro Pro Glu Glu Gln Ala Asn Leu Trp Leu 
                645                 650                 655     
Val Glu Ala Glu Ile Ser Pro Glu Leu Gln Lys Arg Leu Gly Arg Lys 
            660                 665                 670         
Lys Lys Arg Arg Lys Arg Lys Lys Glu Val Cys Pro Leu Ala Pro Pro 
        675                 680                 685             
Pro Glu Leu His Pro Pro Ala Pro Ala Pro Ser Thr Ile Pro Arg Leu 
    690                 695                 700                 
Pro Gln Leu Pro Arg Gln Lys Cys Leu Val Ala Ala Gly Ala Trp Gly 
705                 710                 715                 720 
Ala Gly Asp Ser Cys Arg Gln Gly Ala Trp Thr Leu Val Ser Asn Pro 
                725                 730                 735     
Phe Cys Pro Glu Pro Ser Pro Pro Gln Asp Pro Phe Leu Pro Ser Ala 
            740                 745                 750         
Pro Ala Pro Val Ala Trp Ala His Gly Arg Arg Gln Gly Leu Gly Pro 
        755                 760                 765             
Ile His Ser Arg Thr Asn Leu Met Asp Thr Glu Leu Met Asp Ala Asp 
    770                 775                 780                 
Ser Asp Phe Leu Ile Asn Gly Ser Gly Ser Gly Ser Gly Met Val Ser 
785                 790                 795                 800 
Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val Glu Leu 
                805                 810                 815     
Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly Glu Gly Glu 
            820                 825                 830         
Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile Cys Thr Thr 
        835                 840                 845             
Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Leu Thr Tyr 
    850                 855                 860                 
Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys Gln His Asp 
865                 870                 875                 880 
Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg Thr Ile 
                885                 890                 895     
Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val Lys Phe 
            900                 905                 910         
Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile Asp Phe 
        915                 920                 925             
Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn Tyr Asn 
    930                 935                 940                 
Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn Gly Ile Lys 
945                 950                 955                 960 
Val Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser Val Gln Leu 
                965                 970                 975     
Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro Val Leu 
            980                 985                 990         
Leu Pro Asp Asn His Tyr Leu Ser  Thr Gln Ser Ala Leu  Ser Lys Asp 
        995                 1000                 1005             
Pro Asn  Glu Lys Arg Asp His  Met Val Leu Leu Glu  Phe Val Thr 
    1010                 1015                 1020             
Ala Ala  Gly Ile Thr Leu Gly  Met Asp Glu Leu Tyr  Lys 
    1025                 1030                 1035     
<210>  8
<211>  1036
<212>  PRT
<213>  ˹
<220>  
<223>  SMO D473H-EGFP
<400>  8
Met Ala Ala Ala Arg Pro Ala Arg Gly Pro Glu Leu Pro Leu Leu Gly 
1               5                   10                  15      
Leu Leu Leu Leu Leu Leu Leu Gly Asp Pro Gly Arg Gly Ala Ala Ser 
            20                  25                  30          
Ser Gly Asn Ala Thr Gly Pro Gly Pro Arg Ser Ala Gly Gly Ser Ala 
        35                  40                  45              

Arg Arg Ser Ala Ala Val Thr Gly Pro Pro Pro Pro Leu Ser His Cys 
    50                  55                  60                  
Gly Arg Ala Ala Pro Cys Glu Pro Leu Arg Tyr Asn Val Cys Leu Gly 
65                  70                  75                  80  
Ser Val Leu Pro Tyr Gly Ala Thr Ser Thr Leu Leu Ala Gly Asp Ser 
                85                  90                  95      
Asp Ser Gln Glu Glu Ala His Gly Lys Leu Val Leu Trp Ser Gly Leu 
            100                 105                 110         
Arg Asn Ala Pro Arg Cys Trp Ala Val Ile Gln Pro Leu Leu Cys Ala 
        115                 120                 125             
Val Tyr Met Pro Lys Cys Glu Asn Asp Arg Val Glu Leu Pro Ser Arg 
    130                 135                 140                 
Thr Leu Cys Gln Ala Thr Arg Gly Pro Cys Ala Ile Val Glu Arg Glu 
145                 150                 155                 160 
Arg Gly Trp Pro Asp Phe Leu Arg Cys Thr Pro Asp Arg Phe Pro Glu 
                165                 170                 175     
Gly Cys Thr Asn Glu Val Gln Asn Ile Lys Phe Asn Ser Ser Gly Gln 
            180                 185                 190         
Cys Glu Val Pro Leu Val Arg Thr Asp Asn Pro Lys Ser Trp Tyr Glu 
        195                 200                 205             
Asp Val Glu Gly Cys Gly Ile Gln Cys Gln Asn Pro Leu Phe Thr Glu 
    210                 215                 220                 
Ala Glu His Gln Asp Met His Ser Tyr Ile Ala Ala Phe Gly Ala Val 
225                 230                 235                 240 
Thr Gly Leu Cys Thr Leu Phe Thr Leu Ala Thr Phe Val Ala Asp Trp 
                245                 250                 255     
Arg Asn Ser Asn Arg Tyr Pro Ala Val Ile Leu Phe Tyr Val Asn Ala 
            260                 265                 270         
Cys Phe Phe Val Gly Ser Ile Gly Trp Leu Ala Gln Phe Met Asp Gly 
        275                 280                 285             
Ala Arg Arg Glu Ile Val Cys Arg Ala Asp Gly Thr Met Arg Leu Gly 
    290                 295                 300                 
Glu Pro Thr Ser Asn Glu Thr Leu Ser Cys Val Ile Ile Phe Val Ile 
305                 310                 315                 320 
Val Tyr Tyr Ala Leu Met Ala Gly Val Val Trp Phe Val Val Leu Thr 
                325                 330                 335     
Tyr Ala Trp His Thr Ser Phe Lys Ala Leu Gly Thr Thr Tyr Gln Pro 
            340                 345                 350         
Leu Ser Gly Lys Thr Ser Tyr Phe His Leu Leu Thr Trp Ser Leu Pro 
        355                 360                 365             
Phe Val Leu Thr Val Ala Ile Leu Ala Val Ala Gln Val Asp Gly Asp 
    370                 375                 380                 
Ser Val Ser Gly Ile Cys Phe Val Gly Tyr Lys Asn Tyr Arg Tyr Arg 
385                 390                 395                 400 
Ala Gly Phe Val Leu Ala Pro Ile Gly Leu Val Leu Ile Val Gly Gly 
                405                 410                 415     
Tyr Phe Leu Ile Arg Gly Val Met Thr Leu Phe Ser Ile Lys Ser Asn 
            420                 425                 430         
His Pro Gly Leu Leu Ser Glu Lys Ala Ala Ser Lys Ile Asn Glu Thr 
        435                 440                 445             
Met Leu Arg Leu Gly Ile Phe Gly Phe Leu Ala Phe Gly Phe Val Leu 
    450                 455                 460                 
Ile Thr Phe Ser Cys His Phe Tyr His Phe Phe Asn Gln Ala Glu Trp 
465                 470                 475                 480 
Glu Arg Ser Phe Arg Asp Tyr Val Leu Cys Gln Ala Asn Val Thr Ile 
                485                 490                 495     
Gly Leu Pro Thr Lys Gln Pro Ile Pro Asp Cys Glu Ile Lys Asn Arg 
            500                 505                 510         
Pro Ser Leu Leu Val Glu Lys Ile Asn Leu Phe Ala Met Phe Gly Thr 
        515                 520                 525             
Gly Ile Ala Met Ser Thr Trp Val Trp Thr Lys Ala Thr Leu Leu Ile 
    530                 535                 540                 
Trp Arg Arg Thr Trp Cys Arg Leu Thr Gly Gln Ser Asp Asp Glu Pro 
545                 550                 555                 560 

Lys Arg Ile Lys Lys Ser Lys Met Ile Ala Lys Ala Phe Ser Lys Arg 
                565                 570                 575     
His Glu Leu Leu Gln Asn Pro Gly Gln Glu Leu Ser Phe Ser Met His 
            580                 585                 590         
Thr Val Ser His Asp Gly Pro Val Ala Gly Leu Ala Phe Asp Leu Asn 
        595                 600                 605             
Glu Pro Ser Ala Asp Val Ser Ser Ala Trp Ala Gln His Val Thr Lys 
    610                 615                 620                 
Met Val Ala Arg Arg Gly Ala Ile Leu Pro Gln Asp Ile Ser Val Thr 
625                 630                 635                 640 
Pro Val Ala Thr Pro Val Pro Pro Glu Glu Gln Ala Asn Leu Trp Leu 
                645                 650                 655     
Val Glu Ala Glu Ile Ser Pro Glu Leu Gln Lys Arg Leu Gly Arg Lys 
            660                 665                 670         
Lys Lys Arg Arg Lys Arg Lys Lys Glu Val Cys Pro Leu Ala Pro Pro 
        675                 680                 685             
Pro Glu Leu His Pro Pro Ala Pro Ala Pro Ser Thr Ile Pro Arg Leu 
    690                 695                 700                 
Pro Gln Leu Pro Arg Gln Lys Cys Leu Val Ala Ala Gly Ala Trp Gly 
705                 710                 715                 720 
Ala Gly Asp Ser Cys Arg Gln Gly Ala Trp Thr Leu Val Ser Asn Pro 
                725                 730                 735     
Phe Cys Pro Glu Pro Ser Pro Pro Gln Asp Pro Phe Leu Pro Ser Ala 
            740                 745                 750         
Pro Ala Pro Val Ala Trp Ala His Gly Arg Arg Gln Gly Leu Gly Pro 
        755                 760                 765             
Ile His Ser Arg Thr Asn Leu Met Asp Thr Glu Leu Met Asp Ala Asp 
    770                 775                 780                 
Ser Asp Phe Leu Ile Asn Gly Ser Gly Ser Gly Ser Gly Met Val Ser 
785                 790                 795                 800 
Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val Glu Leu 
                805                 810                 815     
Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly Glu Gly Glu 
            820                 825                 830         
Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile Cys Thr Thr 
        835                 840                 845             
Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Leu Thr Tyr 
    850                 855                 860                 
Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys Gln His Asp 
865                 870                 875                 880 
Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg Thr Ile 
                885                 890                 895     
Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val Lys Phe 
            900                 905                 910         
Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile Asp Phe 
        915                 920                 925             
Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn Tyr Asn 
    930                 935                 940                 
Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn Gly Ile Lys 
945                 950                 955                 960 
Val Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser Val Gln Leu 
                965                 970                 975     
Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro Val Leu 
            980                 985                 990         
Leu Pro Asp Asn His Tyr Leu Ser  Thr Gln Ser Ala Leu  Ser Lys Asp 
        995                 1000                 1005             
Pro Asn  Glu Lys Arg Asp His  Met Val Leu Leu Glu  Phe Val Thr 
    1010                 1015                 1020             
Ala Ala  Gly Ile Thr Leu Gly  Met Asp Glu Leu Tyr  Lys 
    1025                 1030                 1035     
<210>  9
<211>  1036
<212>  PRT
<213>  ˹
<220>  
<223>  SMO W535L-EGFP
<400>  9
Met Ala Ala Ala Arg Pro Ala Arg Gly Pro Glu Leu Pro Leu Leu Gly 
1               5                   10                  15      
Leu Leu Leu Leu Leu Leu Leu Gly Asp Pro Gly Arg Gly Ala Ala Ser 
            20                  25                  30          
Ser Gly Asn Ala Thr Gly Pro Gly Pro Arg Ser Ala Gly Gly Ser Ala 
        35                  40                  45              
Arg Arg Ser Ala Ala Val Thr Gly Pro Pro Pro Pro Leu Ser His Cys 
    50                  55                  60                  
Gly Arg Ala Ala Pro Cys Glu Pro Leu Arg Tyr Asn Val Cys Leu Gly 
65                  70                  75                  80  
Ser Val Leu Pro Tyr Gly Ala Thr Ser Thr Leu Leu Ala Gly Asp Ser 
                85                  90                  95      
Asp Ser Gln Glu Glu Ala His Gly Lys Leu Val Leu Trp Ser Gly Leu 
            100                 105                 110         
Arg Asn Ala Pro Arg Cys Trp Ala Val Ile Gln Pro Leu Leu Cys Ala 
        115                 120                 125             
Val Tyr Met Pro Lys Cys Glu Asn Asp Arg Val Glu Leu Pro Ser Arg 
    130                 135                 140                 
Thr Leu Cys Gln Ala Thr Arg Gly Pro Cys Ala Ile Val Glu Arg Glu 
145                 150                 155                 160 
Arg Gly Trp Pro Asp Phe Leu Arg Cys Thr Pro Asp Arg Phe Pro Glu 
                165                 170                 175     
Gly Cys Thr Asn Glu Val Gln Asn Ile Lys Phe Asn Ser Ser Gly Gln 
            180                 185                 190         
Cys Glu Val Pro Leu Val Arg Thr Asp Asn Pro Lys Ser Trp Tyr Glu 
        195                 200                 205             
Asp Val Glu Gly Cys Gly Ile Gln Cys Gln Asn Pro Leu Phe Thr Glu 
    210                 215                 220                 
Ala Glu His Gln Asp Met His Ser Tyr Ile Ala Ala Phe Gly Ala Val 
225                 230                 235                 240 
Thr Gly Leu Cys Thr Leu Phe Thr Leu Ala Thr Phe Val Ala Asp Trp 
                245                 250                 255     
Arg Asn Ser Asn Arg Tyr Pro Ala Val Ile Leu Phe Tyr Val Asn Ala 
            260                 265                 270         
Cys Phe Phe Val Gly Ser Ile Gly Trp Leu Ala Gln Phe Met Asp Gly 
        275                 280                 285             
Ala Arg Arg Glu Ile Val Cys Arg Ala Asp Gly Thr Met Arg Leu Gly 
    290                 295                 300                 
Glu Pro Thr Ser Asn Glu Thr Leu Ser Cys Val Ile Ile Phe Val Ile 
305                 310                 315                 320 
Val Tyr Tyr Ala Leu Met Ala Gly Val Val Trp Phe Val Val Leu Thr 
                325                 330                 335     
Tyr Ala Trp His Thr Ser Phe Lys Ala Leu Gly Thr Thr Tyr Gln Pro 
            340                 345                 350         
Leu Ser Gly Lys Thr Ser Tyr Phe His Leu Leu Thr Trp Ser Leu Pro 
        355                 360                 365             
Phe Val Leu Thr Val Ala Ile Leu Ala Val Ala Gln Val Asp Gly Asp 
    370                 375                 380                 
Ser Val Ser Gly Ile Cys Phe Val Gly Tyr Lys Asn Tyr Arg Tyr Arg 
385                 390                 395                 400 
Ala Gly Phe Val Leu Ala Pro Ile Gly Leu Val Leu Ile Val Gly Gly 
                405                 410                 415     
Tyr Phe Leu Ile Arg Gly Val Met Thr Leu Phe Ser Ile Lys Ser Asn 
            420                 425                 430         
His Pro Gly Leu Leu Ser Glu Lys Ala Ala Ser Lys Ile Asn Glu Thr 
        435                 440                 445             
Met Leu Arg Leu Gly Ile Phe Gly Phe Leu Ala Phe Gly Phe Val Leu 
    450                 455                 460                 
Ile Thr Phe Ser Cys His Phe Tyr Asp Phe Phe Asn Gln Ala Glu Trp 
465                 470                 475                 480 
Glu Arg Ser Phe Arg Asp Tyr Val Leu Cys Gln Ala Asn Val Thr Ile 
                485                 490                 495     
Gly Leu Pro Thr Lys Gln Pro Ile Pro Asp Cys Glu Ile Lys Asn Arg 
            500                 505                 510         
Pro Ser Leu Leu Val Glu Lys Ile Asn Leu Phe Ala Met Phe Gly Thr 
        515                 520                 525             
Gly Ile Ala Met Ser Thr Leu Val Trp Thr Lys Ala Thr Leu Leu Ile 
    530                 535                 540                 
Trp Arg Arg Thr Trp Cys Arg Leu Thr Gly Gln Ser Asp Asp Glu Pro 
545                 550                 555                 560 
Lys Arg Ile Lys Lys Ser Lys Met Ile Ala Lys Ala Phe Ser Lys Arg 
                565                 570                 575     
His Glu Leu Leu Gln Asn Pro Gly Gln Glu Leu Ser Phe Ser Met His 
            580                 585                 590         
Thr Val Ser His Asp Gly Pro Val Ala Gly Leu Ala Phe Asp Leu Asn 
        595                 600                 605             
Glu Pro Ser Ala Asp Val Ser Ser Ala Trp Ala Gln His Val Thr Lys 
    610                 615                 620                 
Met Val Ala Arg Arg Gly Ala Ile Leu Pro Gln Asp Ile Ser Val Thr 
625                 630                 635                 640 
Pro Val Ala Thr Pro Val Pro Pro Glu Glu Gln Ala Asn Leu Trp Leu 
                645                 650                 655     
Val Glu Ala Glu Ile Ser Pro Glu Leu Gln Lys Arg Leu Gly Arg Lys 
            660                 665                 670         
Lys Lys Arg Arg Lys Arg Lys Lys Glu Val Cys Pro Leu Ala Pro Pro 
        675                 680                 685             
Pro Glu Leu His Pro Pro Ala Pro Ala Pro Ser Thr Ile Pro Arg Leu 
    690                 695                 700                 
Pro Gln Leu Pro Arg Gln Lys Cys Leu Val Ala Ala Gly Ala Trp Gly 
705                 710                 715                 720 
Ala Gly Asp Ser Cys Arg Gln Gly Ala Trp Thr Leu Val Ser Asn Pro 
                725                 730                 735     
Phe Cys Pro Glu Pro Ser Pro Pro Gln Asp Pro Phe Leu Pro Ser Ala 
            740                 745                 750         
Pro Ala Pro Val Ala Trp Ala His Gly Arg Arg Gln Gly Leu Gly Pro 
        755                 760                 765             
Ile His Ser Arg Thr Asn Leu Met Asp Thr Glu Leu Met Asp Ala Asp 
    770                 775                 780                 
Ser Asp Phe Leu Ile Asn Gly Ser Gly Ser Gly Ser Gly Met Val Ser 
785                 790                 795                 800 
Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val Glu Leu 
                805                 810                 815     
Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly Glu Gly Glu 
            820                 825                 830         
Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile Cys Thr Thr 
        835                 840                 845             
Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Leu Thr Tyr 
    850                 855                 860                 
Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys Gln His Asp 
865                 870                 875                 880 
Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg Thr Ile 
                885                 890                 895     
Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val Lys Phe 
            900                 905                 910         
Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile Asp Phe 
        915                 920                 925             
Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn Tyr Asn 
    930                 935                 940                 
Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn Gly Ile Lys 
945                 950                 955                 960 
Val Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser Val Gln Leu 
                965                 970                 975     
Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro Val Leu 
            980                 985                 990         
Leu Pro Asp Asn His Tyr Leu Ser  Thr Gln Ser Ala Leu  Ser Lys Asp 
        995                 1000                 1005             
Pro Asn  Glu Lys Arg Asp His  Met Val Leu Leu Glu  Phe Val Thr 
    1010                 1015                 1020             
Ala Ala  Gly Ile Thr Leu Gly  Met Asp Glu Leu Tyr  Lys 
    1025                 1030                 1035     
<210>  10
<211>  1358
<212>  PRT
<213>  ˹
<220>  
<223>  SMO D473H-P2A-өөø
<400>  10
Met Ala Ala Ala Arg Pro Ala Arg Gly Pro Glu Leu Pro Leu Leu Gly 
1               5                   10                  15      
Leu Leu Leu Leu Leu Leu Leu Gly Asp Pro Gly Arg Gly Ala Ala Ser 
            20                  25                  30          
Ser Gly Asn Ala Thr Gly Pro Gly Pro Arg Ser Ala Gly Gly Ser Ala 
        35                  40                  45              
Arg Arg Ser Ala Ala Val Thr Gly Pro Pro Pro Pro Leu Ser His Cys 
    50                  55                  60                  
Gly Arg Ala Ala Pro Cys Glu Pro Leu Arg Tyr Asn Val Cys Leu Gly 
65                  70                  75                  80  
Ser Val Leu Pro Tyr Gly Ala Thr Ser Thr Leu Leu Ala Gly Asp Ser 
                85                  90                  95      
Asp Ser Gln Glu Glu Ala His Gly Lys Leu Val Leu Trp Ser Gly Leu 
            100                 105                 110         
Arg Asn Ala Pro Arg Cys Trp Ala Val Ile Gln Pro Leu Leu Cys Ala 
        115                 120                 125             
Val Tyr Met Pro Lys Cys Glu Asn Asp Arg Val Glu Leu Pro Ser Arg 
    130                 135                 140                 
Thr Leu Cys Gln Ala Thr Arg Gly Pro Cys Ala Ile Val Glu Arg Glu 
145                 150                 155                 160 
Arg Gly Trp Pro Asp Phe Leu Arg Cys Thr Pro Asp Arg Phe Pro Glu 
                165                 170                 175     
Gly Cys Thr Asn Glu Val Gln Asn Ile Lys Phe Asn Ser Ser Gly Gln 
            180                 185                 190         
Cys Glu Val Pro Leu Val Arg Thr Asp Asn Pro Lys Ser Trp Tyr Glu 
        195                 200                 205             
Asp Val Glu Gly Cys Gly Ile Gln Cys Gln Asn Pro Leu Phe Thr Glu 
    210                 215                 220                 
Ala Glu His Gln Asp Met His Ser Tyr Ile Ala Ala Phe Gly Ala Val 
225                 230                 235                 240 
Thr Gly Leu Cys Thr Leu Phe Thr Leu Ala Thr Phe Val Ala Asp Trp 
                245                 250                 255     
Arg Asn Ser Asn Arg Tyr Pro Ala Val Ile Leu Phe Tyr Val Asn Ala 
            260                 265                 270         
Cys Phe Phe Val Gly Ser Ile Gly Trp Leu Ala Gln Phe Met Asp Gly 
        275                 280                 285             
Ala Arg Arg Glu Ile Val Cys Arg Ala Asp Gly Thr Met Arg Leu Gly 
    290                 295                 300                 
Glu Pro Thr Ser Asn Glu Thr Leu Ser Cys Val Ile Ile Phe Val Ile 
305                 310                 315                 320 
Val Tyr Tyr Ala Leu Met Ala Gly Val Val Trp Phe Val Val Leu Thr 
                325                 330                 335     
Tyr Ala Trp His Thr Ser Phe Lys Ala Leu Gly Thr Thr Tyr Gln Pro 
            340                 345                 350         
Leu Ser Gly Lys Thr Ser Tyr Phe His Leu Leu Thr Trp Ser Leu Pro 
        355                 360                 365             
Phe Val Leu Thr Val Ala Ile Leu Ala Val Ala Gln Val Asp Gly Asp 
    370                 375                 380                 
Ser Val Ser Gly Ile Cys Phe Val Gly Tyr Lys Asn Tyr Arg Tyr Arg 
385                 390                 395                 400 
Ala Gly Phe Val Leu Ala Pro Ile Gly Leu Val Leu Ile Val Gly Gly 
                405                 410                 415     
Tyr Phe Leu Ile Arg Gly Val Met Thr Leu Phe Ser Ile Lys Ser Asn 
            420                 425                 430         
His Pro Gly Leu Leu Ser Glu Lys Ala Ala Ser Lys Ile Asn Glu Thr 
        435                 440                 445             
Met Leu Arg Leu Gly Ile Phe Gly Phe Leu Ala Phe Gly Phe Val Leu 
    450                 455                 460                 
Ile Thr Phe Ser Cys His Phe Tyr His Phe Phe Asn Gln Ala Glu Trp 
465                 470                 475                 480 
Glu Arg Ser Phe Arg Asp Tyr Val Leu Cys Gln Ala Asn Val Thr Ile 
                485                 490                 495     
Gly Leu Pro Thr Lys Gln Pro Ile Pro Asp Cys Glu Ile Lys Asn Arg 
            500                 505                 510         
Pro Ser Leu Leu Val Glu Lys Ile Asn Leu Phe Ala Met Phe Gly Thr 
        515                 520                 525             
Gly Ile Ala Met Ser Thr Trp Val Trp Thr Lys Ala Thr Leu Leu Ile 
    530                 535                 540                 
Trp Arg Arg Thr Trp Cys Arg Leu Thr Gly Gln Ser Asp Asp Glu Pro 
545                 550                 555                 560 
Lys Arg Ile Lys Lys Ser Lys Met Ile Ala Lys Ala Phe Ser Lys Arg 
                565                 570                 575     
His Glu Leu Leu Gln Asn Pro Gly Gln Glu Leu Ser Phe Ser Met His 
            580                 585                 590         
Thr Val Ser His Asp Gly Pro Val Ala Gly Leu Ala Phe Asp Leu Asn 
        595                 600                 605             
Glu Pro Ser Ala Asp Val Ser Ser Ala Trp Ala Gln His Val Thr Lys 
    610                 615                 620                 
Met Val Ala Arg Arg Gly Ala Ile Leu Pro Gln Asp Ile Ser Val Thr 
625                 630                 635                 640 
Pro Val Ala Thr Pro Val Pro Pro Glu Glu Gln Ala Asn Leu Trp Leu 
                645                 650                 655     
Val Glu Ala Glu Ile Ser Pro Glu Leu Gln Lys Arg Leu Gly Arg Lys 
            660                 665                 670         
Lys Lys Arg Arg Lys Arg Lys Lys Glu Val Cys Pro Leu Ala Pro Pro 
        675                 680                 685             
Pro Glu Leu His Pro Pro Ala Pro Ala Pro Ser Thr Ile Pro Arg Leu 
    690                 695                 700                 
Pro Gln Leu Pro Arg Gln Lys Cys Leu Val Ala Ala Gly Ala Trp Gly 
705                 710                 715                 720 
Ala Gly Asp Ser Cys Arg Gln Gly Ala Trp Thr Leu Val Ser Asn Pro 
                725                 730                 735     
Phe Cys Pro Glu Pro Ser Pro Pro Gln Asp Pro Phe Leu Pro Ser Ala 
            740                 745                 750         
Pro Ala Pro Val Ala Trp Ala His Gly Arg Arg Gln Gly Leu Gly Pro 
        755                 760                 765             
Ile His Ser Arg Thr Asn Leu Met Asp Thr Glu Leu Met Asp Ala Asp 
    770                 775                 780                 
Ser Asp Phe Thr Ser Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly 
785                 790                 795                 800 
Asp Val Glu Glu Asn Pro Gly Pro Met Glu Asp Ala Lys Asn Ile Lys 
                805                 810                 815     
Lys Gly Pro Ala Pro Phe Tyr Pro Leu Glu Asp Gly Thr Ala Gly Glu 
            820                 825                 830         
Gln Leu His Lys Ala Met Lys Arg Tyr Ala Leu Val Pro Gly Thr Ile 
        835                 840                 845             
Ala Phe Thr Asp Ala His Ile Glu Val Asp Ile Thr Tyr Ala Glu Tyr 
    850                 855                 860                 
Phe Glu Met Ser Val Arg Leu Ala Glu Ala Met Lys Arg Tyr Gly Leu 
865                 870                 875                 880 
Asn Thr Asn His Arg Ile Val Val Cys Ser Glu Asn Ser Leu Gln Phe 
                885                 890                 895     
Phe Met Pro Val Leu Gly Ala Leu Phe Ile Gly Val Ala Val Ala Pro 
            900                 905                 910         
Ala Asn Asp Ile Tyr Asn Glu Arg Glu Leu Leu Asn Ser Met Gly Ile 
        915                 920                 925             
Ser Gln Pro Thr Val Val Phe Val Ser Lys Lys Gly Leu Gln Lys Ile 
    930                 935                 940                 
Leu Asn Val Gln Lys Lys Leu Pro Ile Ile Gln Lys Ile Ile Ile Met 
945                 950                 955                 960 
Asp Ser Lys Thr Asp Tyr Gln Gly Phe Gln Ser Met Tyr Thr Phe Val 
                965                 970                 975     
Thr Ser His Leu Pro Pro Gly Phe Asn Glu Tyr Asp Phe Val Pro Glu 
            980                 985                 990         
Ser Phe Asp Arg Asp Lys Thr Ile  Ala Leu Ile Met Asn  Ser Ser Gly 
        995                 1000                 1005             
Ser Thr  Gly Leu Pro Lys Gly  Val Ala Leu Pro His  Arg Thr Ala 
    1010                 1015                 1020             
Cys Val  Arg Phe Ser His Ala  Arg Asp Pro Ile Phe  Gly Asn Gln 
    1025                 1030                 1035             
Ile Ile  Pro Asp Thr Ala Ile  Leu Ser Val Val Pro  Phe His His 
    1040                 1045                 1050             
Gly Phe  Gly Met Phe Thr Thr  Leu Gly Tyr Leu Ile  Cys Gly Phe 
    1055                 1060                 1065             
Arg Val  Val Leu Met Tyr Arg  Phe Glu Glu Glu Leu  Phe Leu Arg 
    1070                 1075                 1080             
Ser Leu  Gln Asp Tyr Lys Ile  Gln Ser Ala Leu Leu  Val Pro Thr 
    1085                 1090                 1095             
Leu Phe  Ser Phe Phe Ala Lys  Ser Thr Leu Ile Asp  Lys Tyr Asp 
    1100                 1105                 1110             
Leu Ser  Asn Leu His Glu Ile  Ala Ser Gly Gly Ala  Pro Leu Ser 
    1115                 1120                 1125             
Lys Glu  Val Gly Glu Ala Val  Ala Lys Arg Phe His  Leu Pro Gly 
    1130                 1135                 1140             
Ile Arg  Gln Gly Tyr Gly Leu  Thr Glu Thr Thr Ser  Ala Ile Leu 
    1145                 1150                 1155             
Ile Thr  Pro Glu Gly Asp Asp  Lys Pro Gly Ala Val  Gly Lys Val 
    1160                 1165                 1170             
Val Pro  Phe Phe Glu Ala Lys  Val Val Asp Leu Asp  Thr Gly Lys 
    1175                 1180                 1185             
Thr Leu  Gly Val Asn Gln Arg  Gly Glu Leu Cys Val  Arg Gly Pro 
    1190                 1195                 1200             
Met Ile  Met Ser Gly Tyr Val  Asn Asn Pro Glu Ala  Thr Asn Ala 
    1205                 1210                 1215             
Leu Ile  Asp Lys Asp Gly Trp  Leu His Ser Gly Asp  Ile Ala Tyr 
    1220                 1225                 1230             
Trp Asp  Glu Asp Glu His Phe  Phe Ile Val Asp Arg  Leu Lys Ser 
    1235                 1240                 1245             
Leu Ile  Lys Tyr Lys Gly Tyr  Gln Val Ala Pro Ala  Glu Leu Glu 
    1250                 1255                 1260             
Ser Ile  Leu Leu Gln His Pro  Asn Ile Phe Asp Ala  Gly Val Ala 
    1265                 1270                 1275             
Gly Leu  Pro Asp Asp Asp Ala  Gly Glu Leu Pro Ala  Ala Val Val 
    1280                 1285                 1290             
Val Leu  Glu His Gly Lys Thr  Met Thr Glu Lys Glu  Ile Val Asp 
    1295                 1300                 1305             
Tyr Val  Ala Ser Gln Val Thr  Thr Ala Lys Lys Leu  Arg Gly Gly 
    1310                 1315                 1320             
Val Val  Phe Val Asp Glu Val  Pro Lys Gly Leu Thr  Gly Lys Leu 
    1325                 1330                 1335             
Asp Ala  Arg Lys Ile Arg Glu  Ile Leu Ile Lys Ala  Lys Lys Gly 
    1340                 1345                 1350             
Gly Lys  Ile Ala Val 
    1355             
<210>  11
<211>  2157
<212>  PRT
<213>  ˹
<220>  
<223>  Gli2-P2A-өөø
<400>  11
Met Glu Thr Ser Ala Ser Ala Thr Ala Ser Glu Lys Gln Glu Ala Lys 
1               5                   10                  15      
Ser Gly Ile Leu Glu Ala Ala Gly Phe Pro Asp Pro Gly Lys Lys Ala 
            20                  25                  30          
Ser Pro Leu Val Val Ala Ala Ala Ala Ala Ala Ala Val Ala Ala Gln 
        35                  40                  45              
Gly Val Pro Gln His Leu Leu Pro Pro Phe His Ala Pro Leu Pro Ile 
    50                  55                  60                  
Asp Met Arg His Gln Glu Gly Arg Tyr His Tyr Glu Pro His Ser Val 
65                  70                  75                  80  
His Gly Val His Gly Pro Pro Ala Leu Ser Gly Ser Pro Val Ile Ser 
                85                  90                  95      
Asp Ile Ser Leu Ile Arg Leu Ser Pro His Pro Ala Gly Pro Gly Glu 
            100                 105                 110         
Ser Pro Phe Asn Ala Pro His Pro Tyr Val Asn Pro His Met Glu His 
        115                 120                 125             
Tyr Leu Arg Ser Val His Ser Ser Pro Thr Leu Ser Met Ile Ser Ala 
    130                 135                 140                 
Ala Arg Gly Leu Ser Pro Ala Asp Val Ala Gln Glu His Leu Lys Glu 
145                 150                 155                 160 
Arg Gly Leu Phe Gly Leu Pro Ala Pro Gly Thr Thr Pro Ser Asp Tyr 
                165                 170                 175     
Tyr His Gln Met Thr Leu Val Ala Gly His Pro Ala Pro Tyr Gly Asp 
            180                 185                 190         
Leu Leu Met Gln Ser Gly Gly Ala Ala Ser Ala Pro His Leu His Asp 
        195                 200                 205             
Tyr Leu Asn Pro Val Asp Val Ser Arg Phe Ser Ser Pro Arg Val Thr 
    210                 215                 220                 
Pro Arg Leu Ser Arg Lys Arg Ala Leu Ser Ile Ser Pro Leu Ser Asp 
225                 230                 235                 240 
Ala Ser Leu Asp Leu Gln Arg Met Ile Arg Thr Ser Pro Asn Ser Leu 
                245                 250                 255     
Val Ala Tyr Ile Asn Asn Ser Arg Ser Ser Ser Ala Ala Ser Gly Ser 
            260                 265                 270         
Tyr Gly His Leu Ser Ala Gly Ala Leu Ser Pro Ala Phe Thr Phe Pro 
        275                 280                 285             
His Pro Ile Asn Pro Val Ala Tyr Gln Gln Ile Leu Ser Gln Gln Arg 
    290                 295                 300                 
Gly Leu Gly Ser Ala Phe Gly His Thr Pro Pro Leu Ile Gln Pro Ser 
305                 310                 315                 320 
Pro Thr Phe Leu Ala Gln Gln Pro Met Ala Leu Thr Ser Ile Asn Ala 
                325                 330                 335     
Thr Pro Thr Gln Leu Ser Ser Ser Ser Asn Cys Leu Ser Asp Thr Asn 
            340                 345                 350         
Gln Asn Lys Gln Ser Ser Glu Ser Ala Val Ser Ser Thr Val Asn Pro 
        355                 360                 365             
Val Ala Ile His Lys Arg Ser Lys Val Lys Thr Glu Pro Glu Gly Leu 
    370                 375                 380                 
Arg Pro Ala Ser Pro Leu Ala Leu Thr Gln Gly Gln Val Ser Gly His 
385                 390                 395                 400 
Gly Ser Cys Gly Cys Ala Leu Pro Leu Ser Gln Glu Gln Leu Ala Asp 
                405                 410                 415     
Leu Lys Glu Asp Leu Asp Arg Asp Asp Cys Lys Gln Glu Ala Glu Val 
            420                 425                 430         
Val Ile Tyr Glu Thr Asn Cys His Trp Glu Asp Cys Thr Lys Glu Tyr 
        435                 440                 445             
Asp Thr Gln Glu Gln Leu Val His His Ile Asn Asn Glu His Ile His 
    450                 455                 460                 
Gly Glu Lys Lys Glu Phe Val Cys Arg Trp Gln Ala Cys Thr Arg Glu 
465                 470                 475                 480 
Gln Lys Pro Phe Lys Ala Gln Tyr Met Leu Val Val His Met Arg Arg 
                485                 490                 495     
His Thr Gly Glu Lys Pro His Lys Cys Thr Phe Glu Gly Cys Ser Lys 
            500                 505                 510         
Ala Tyr Ser Arg Leu Glu Asn Leu Lys Thr His Leu Arg Ser His Thr 
        515                 520                 525             
Gly Glu Lys Pro Tyr Val Cys Glu His Glu Gly Cys Asn Lys Ala Phe 
    530                 535                 540                 
Ser Asn Ala Ser Asp Arg Ala Lys His Gln Asn Arg Thr His Ser Asn 
545                 550                 555                 560 
Glu Lys Pro Tyr Ile Cys Lys Ile Pro Gly Cys Thr Lys Arg Tyr Thr 
                565                 570                 575     
Asp Pro Ser Ser Leu Arg Lys His Val Lys Thr Val His Gly Pro Asp 
            580                 585                 590         
Ala His Val Thr Lys Lys Gln Arg Asn Asp Val His Leu Arg Thr Pro 
        595                 600                 605             
Leu Leu Lys Glu Asn Gly Asp Ser Glu Ala Gly Thr Glu Pro Gly Gly 
    610                 615                 620                 
Pro Glu Ser Thr Glu Ala Ser Ser Thr Ser Gln Ala Val Glu Asp Cys 
625                 630                 635                 640 
Leu His Val Arg Ala Ile Lys Thr Glu Ser Ser Gly Leu Cys Gln Ser 
                645                 650                 655     
Ser Pro Gly Ala Gln Ser Ser Cys Ser Ser Glu Pro Ser Pro Leu Gly 
            660                 665                 670         
Ser Ala Pro Asn Asn Asp Ser Gly Val Glu Met Pro Gly Thr Gly Pro 
        675                 680                 685             
Gly Ser Leu Gly Asp Leu Thr Ala Leu Asp Asp Thr Pro Pro Gly Ala 
    690                 695                 700                 
Asp Thr Ser Ala Leu Ala Ala Pro Ser Ala Gly Gly Leu Gln Leu Arg 
705                 710                 715                 720 
Lys His Met Thr Thr Met His Arg Phe Glu Gln Leu Lys Lys Glu Lys 
                725                 730                 735     
Leu Lys Ser Leu Lys Asp Ser Cys Ser Trp Ala Gly Pro Thr Pro His 
            740                 745                 750         
Thr Arg Asn Thr Lys Leu Pro Pro Leu Pro Gly Ser Gly Ser Ile Leu 
        755                 760                 765             
Glu Asn Phe Ser Gly Ser Gly Gly Gly Gly Pro Ala Gly Leu Leu Pro 
    770                 775                 780                 
Asn Pro Arg Leu Ser Glu Leu Ser Ala Ser Glu Val Thr Met Leu Ser 
785                 790                 795                 800 
Gln Leu Gln Glu Arg Arg Asp Ser Ser Thr Ser Thr Val Ser Ser Ala 
                805                 810                 815     
Tyr Thr Val Ser Arg Arg Ser Ser Gly Ile Ser Pro Tyr Phe Ser Ser 
            820                 825                 830         
Arg Arg Ser Ser Glu Ala Ser Pro Leu Gly Ala Gly Arg Pro His Asn 
        835                 840                 845             
Ala Ser Ser Ala Asp Ser Tyr Asp Pro Ile Ser Thr Asp Ala Ser Arg 
    850                 855                 860                 
Arg Ser Ser Glu Ala Ser Gln Cys Ser Gly Gly Ser Gly Leu Leu Asn 
865                 870                 875                 880 
Leu Thr Pro Ala Gln Gln Tyr Ser Leu Arg Ala Lys Tyr Ala Ala Ala 
                885                 890                 895     
Thr Gly Gly Pro Pro Pro Thr Pro Leu Pro Gly Leu Glu Arg Met Ser 
            900                 905                 910         
Leu Arg Thr Arg Leu Ala Leu Leu Asp Ala Pro Glu Arg Thr Leu Pro 
        915                 920                 925             
Ala Gly Cys Pro Arg Pro Leu Gly Pro Arg Arg Gly Ser Asp Gly Pro 
    930                 935                 940                 
Thr Tyr Gly His Gly His Ala Gly Ala Ala Pro Ala Phe Pro His Glu 
945                 950                 955                 960 
Ala Pro Gly Gly Gly Ala Arg Arg Ala Ser Asp Pro Val Arg Arg Pro 
                965                 970                 975     
Asp Ala Leu Ser Leu Pro Arg Val Gln Arg Phe His Ser Thr His Asn 
            980                 985                 990         
Val Asn Pro Gly Pro Leu Pro Pro  Cys Ala Asp Arg Arg  Gly Leu Arg 
        995                 1000                 1005             
Leu Gln  Ser His Pro Ser Thr  Asp Gly Gly Leu Ala  Arg Gly Ala 
    1010                 1015                 1020             
Tyr Ser  Pro Arg Pro Pro Ser  Ile Ser Glu Asn Val  Ala Met Glu 
    1025                 1030                 1035             
Ala Val  Ala Ala Gly Val Asp  Gly Ala Gly Pro Glu  Ala Asp Leu 
    1040                 1045                 1050             
Gly Leu  Pro Glu Asp Asp Leu  Val Leu Pro Asp Asp  Val Val Gln 
    1055                 1060                 1065             
Tyr Ile  Lys Ala His Ala Ser  Gly Ala Leu Asp Glu  Gly Thr Gly 
    1070                 1075                 1080             
Gln Val  Tyr Pro Thr Glu Ser  Thr Gly Phe Ser Asp  Asn Pro Arg 
    1085                 1090                 1095             
Leu Pro  Ser Pro Gly Leu His  Gly Gln Arg Arg Met  Val Ala Ala 
    1100                 1105                 1110             
Asp Ser  Asn Val Gly Pro Ser  Ala Pro Met Leu Gly  Gly Cys Gln 
    1115                 1120                 1125             
Leu Gly  Phe Gly Ala Pro Ser  Ser Leu Asn Lys Asn  Asn Met Pro 
    1130                 1135                 1140             
Val Gln  Trp Asn Glu Val Ser  Ser Gly Thr Val Asp  Ala Leu Ala 
    1145                 1150                 1155             
Ser Gln  Val Lys Pro Pro Pro  Phe Pro Gln Gly Asn  Leu Ala Val 
    1160                 1165                 1170             
Val Gln  Gln Lys Pro Ala Phe  Gly Gln Tyr Pro Gly  Tyr Ser Pro 
    1175                 1180                 1185             
Gln Gly  Leu Gln Ala Ser Pro  Gly Gly Leu Asp Ser  Thr Gln Pro 
    1190                 1195                 1200             
His Leu  Gln Pro Arg Ser Gly  Ala Pro Ser Gln Gly  Ile Pro Arg 
    1205                 1210                 1215             
Val Asn  Tyr Met Gln Gln Leu  Arg Gln Pro Val Ala  Gly Ser Gln 
    1220                 1225                 1230             
Cys Pro  Gly Met Thr Thr Thr  Met Ser Pro His Ala  Cys Tyr Gly 
    1235                 1240                 1245             
Gln Val  His Pro Gln Leu Ser  Pro Ser Thr Ile Ser  Gly Ala Leu 
    1250                 1255                 1260             
Asn Gln  Phe Pro Gln Ser Cys  Ser Asn Met Pro Ala  Lys Pro Gly 
    1265                 1270                 1275             
His Leu  Gly His Pro Gln Gln  Thr Glu Val Ala Pro  Asp Pro Thr 
    1280                 1285                 1290             
Thr Met  Gly Asn Arg His Arg  Glu Leu Gly Val Pro  Asp Ser Ala 
    1295                 1300                 1305             
Leu Ala  Gly Val Pro Pro Pro  His Pro Val Gln Ser  Tyr Pro Gln 
    1310                 1315                 1320             
Gln Ser  His His Leu Ala Ala  Ser Met Ser Gln Glu  Gly Tyr His 
    1325                 1330                 1335             
Gln Val  Pro Ser Leu Leu Pro  Ala Arg Gln Pro Gly  Phe Met Glu 
    1340                 1345                 1350             
Pro Gln  Thr Gly Pro Met Gly  Val Ala Thr Ala Gly  Phe Gly Leu 
    1355                 1360                 1365             
Val Gln  Pro Arg Pro Pro Leu  Glu Pro Ser Pro Thr  Gly Arg His 
    1370                 1375                 1380             
Arg Gly  Val Arg Ala Val Gln  Gln Gln Leu Ala Tyr  Ala Arg Ala 
    1385                 1390                 1395             
Thr Gly  His Ala Met Ala Ala  Met Pro Ser Ser Gln  Glu Thr Ala 
    1400                 1405                 1410             
Glu Ala  Val Pro Lys Gly Ala  Met Gly Asn Met Gly  Ser Val Pro 
    1415                 1420                 1425             
Pro Gln  Pro Pro Pro Gln Asp  Ala Gly Gly Ala Pro  Asp His Ser 
    1430                 1435                 1440             
Met Leu  Tyr Tyr Tyr Gly Gln  Ile His Met Tyr Glu  Gln Asp Gly 
    1445                 1450                 1455             
Gly Leu  Glu Asn Leu Gly Ser  Cys Gln Val Met Arg  Ser Gln Pro 
    1460                 1465                 1470             
Pro Gln  Pro Gln Ala Cys Gln  Asp Ser Ile Gln Pro  Gln Pro Leu 
    1475                 1480                 1485             
Pro Ser  Pro Gly Val Asn Gln  Val Ser Ser Thr Val  Asp Ser Gln 
    1490                 1495                 1500             
Leu Leu  Glu Ala Pro Gln Ile  Asp Phe Asp Ala Ile  Met Asp Asp 
    1505                 1510                 1515             
Gly Asp  His Ser Ser Leu Phe  Ser Gly Ala Leu Ser  Pro Ser Leu 
    1520                 1525                 1530             
Leu His  Ser Leu Ser Gln Asn  Ser Ser Arg Leu Thr  Thr Pro Arg 
    1535                 1540                 1545             
Asn Ser  Leu Thr Leu Pro Ser  Ile Pro Ala Gly Ile  Ser Asn Met 
    1550                 1555                 1560             
Ala Val  Gly Asp Met Ser Ser  Met Leu Thr Ser Leu  Ala Glu Glu 
    1565                 1570                 1575             
Ser Lys  Phe Leu Asn Met Met  Thr Thr Ser Ala Thr  Asn Phe Ser 
    1580                 1585                 1590             
Leu Leu  Lys Gln Ala Gly Asp  Val Glu Glu Asn Pro  Gly Pro Met 
    1595                 1600                 1605             
Glu Asp  Ala Lys Asn Ile Lys  Lys Gly Pro Ala Pro  Phe Tyr Pro 
    1610                 1615                 1620             
Leu Glu  Asp Gly Thr Ala Gly  Glu Gln Leu His Lys  Ala Met Lys 
    1625                 1630                 1635             
Arg Tyr  Ala Leu Val Pro Gly  Thr Ile Ala Phe Thr  Asp Ala His 
    1640                 1645                 1650             
Ile Glu  Val Asp Ile Thr Tyr  Ala Glu Tyr Phe Glu  Met Ser Val 
    1655                 1660                 1665             
Arg Leu  Ala Glu Ala Met Lys  Arg Tyr Gly Leu Asn  Thr Asn His 
    1670                 1675                 1680             
Arg Ile  Val Val Cys Ser Glu  Asn Ser Leu Gln Phe  Phe Met Pro 
    1685                 1690                 1695             
Val Leu  Gly Ala Leu Phe Ile  Gly Val Ala Val Ala  Pro Ala Asn 
    1700                 1705                 1710             
Asp Ile  Tyr Asn Glu Arg Glu  Leu Leu Asn Ser Met  Gly Ile Ser 
    1715                 1720                 1725             
Gln Pro  Thr Val Val Phe Val  Ser Lys Lys Gly Leu  Gln Lys Ile 
    1730                 1735                 1740             
Leu Asn  Val Gln Lys Lys Leu  Pro Ile Ile Gln Lys  Ile Ile Ile 
    1745                 1750                 1755             
Met Asp  Ser Lys Thr Asp Tyr  Gln Gly Phe Gln Ser  Met Tyr Thr 
    1760                 1765                 1770             
Phe Val  Thr Ser His Leu Pro  Pro Gly Phe Asn Glu  Tyr Asp Phe 
    1775                 1780                 1785             
Val Pro  Glu Ser Phe Asp Arg  Asp Lys Thr Ile Ala  Leu Ile Met 
    1790                 1795                 1800             
Asn Ser  Ser Gly Ser Thr Gly  Leu Pro Lys Gly Val  Ala Leu Pro 
    1805                 1810                 1815             
His Arg  Thr Ala Cys Val Arg  Phe Ser His Ala Arg  Asp Pro Ile 
    1820                 1825                 1830             
Phe Gly  Asn Gln Ile Ile Pro  Asp Thr Ala Ile Leu  Ser Val Val 
    1835                 1840                 1845             
Pro Phe  His His Gly Phe Gly  Met Phe Thr Thr Leu  Gly Tyr Leu 
    1850                 1855                 1860             
Ile Cys  Gly Phe Arg Val Val  Leu Met Tyr Arg Phe  Glu Glu Glu 
    1865                 1870                 1875             
Leu Phe  Leu Arg Ser Leu Gln  Asp Tyr Lys Ile Gln  Ser Ala Leu 
    1880                 1885                 1890             
Leu Val  Pro Thr Leu Phe Ser  Phe Phe Ala Lys Ser  Thr Leu Ile 
    1895                 1900                 1905             
Asp Lys  Tyr Asp Leu Ser Asn  Leu His Glu Ile Ala  Ser Gly Gly 
    1910                 1915                 1920             
Ala Pro  Leu Ser Lys Glu Val  Gly Glu Ala Val Ala  Lys Arg Phe 
    1925                 1930                 1935             
His Leu  Pro Gly Ile Arg Gln  Gly Tyr Gly Leu Thr  Glu Thr Thr 
    1940                 1945                 1950             
Ser Ala  Ile Leu Ile Thr Pro  Glu Gly Asp Asp Lys  Pro Gly Ala 
    1955                 1960                 1965             
Val Gly  Lys Val Val Pro Phe  Phe Glu Ala Lys Val  Val Asp Leu 
    1970                 1975                 1980             
Asp Thr  Gly Lys Thr Leu Gly  Val Asn Gln Arg Gly  Glu Leu Cys 
    1985                 1990                 1995             
Val Arg  Gly Pro Met Ile Met  Ser Gly Tyr Val Asn  Asn Pro Glu 
    2000                 2005                 2010             
Ala Thr  Asn Ala Leu Ile Asp  Lys Asp Gly Trp Leu  His Ser Gly 
    2015                 2020                 2025             
Asp Ile  Ala Tyr Trp Asp Glu  Asp Glu His Phe Phe  Ile Val Asp 
    2030                 2035                 2040             
Arg Leu  Lys Ser Leu Ile Lys  Tyr Lys Gly Tyr Gln  Val Ala Pro 
    2045                 2050                 2055             
Ala Glu  Leu Glu Ser Ile Leu  Leu Gln His Pro Asn  Ile Phe Asp 
    2060                 2065                 2070             
Ala Gly  Val Ala Gly Leu Pro  Asp Asp Asp Ala Gly  Glu Leu Pro 
    2075                 2080                 2085             
Ala Ala  Val Val Val Leu Glu  His Gly Lys Thr Met  Thr Glu Lys 
    2090                 2095                 2100             
Glu Ile  Val Asp Tyr Val Ala  Ser Gln Val Thr Thr  Ala Lys Lys 
    2105                 2110                 2115             
Leu Arg  Gly Gly Val Val Phe  Val Asp Glu Val Pro  Lys Gly Leu 
    2120                 2125                 2130             
Thr Gly  Lys Leu Asp Ala Arg  Lys Ile Arg Glu Ile  Leu Ile Lys 
    2135                 2140                 2145             
Ala Lys  Lys Gly Gly Lys Ile  Ala Val 
    2150                 2155         
<210>  12
<211>  22
<212>  DNA
<213>  ˹
<220>  
<223>  С Gli1
<400>  12
aaggaattcg tgtgccattg gg                                              22
<210>  13
<211>  22
<212>  DNA
<213>  ˹
<220>  
<223>  С Gli1
<400>  13
acatgtaagg cttctcaccc gt                                              22
<210>  14
<211>  21
<212>  DNA
<213>  ˹
<220>  
<223>  С Ptch1
<400>  14
aattctcgac tcactcgtcc a                                               21
<210>  15
<211>  20
<212>  DNA
<213>  ˹
<220>  
<223>  С Ptch1
<400>  15
ctcctcatat ttggggcctt                                                 20
<210>  16
<211>  19
<212>  DNA
<213>  ˹
<220>  
<223>  С Gapdh
<400>  16
tgcccccatg tttgtgatg                                                  19
<210>  17
<211>  19
<212>  DNA
<213>  ˹
<220>  
<223>  С Gapdh
<400>  17
tgtggtcatg agcccttcc                                                  19
<210>  18
<211>  23
<212>  DNA
<213>  ˹
<220>  
<223>   GLI1
<400>  18
ttggagaagc cgagccgagt atc                                             23
<210>  19
<211>  26
<212>  DNA
<213>  ˹
<220>  
<223>   GLI1
<400>  19
gagtagacag aggttgggag gtaagg                                          26
<210>  20
<211>  21
<212>  DNA
<213>  ˹
<220>  
<223>  GLI2
<400>  20
catggagcac tacctccgtt c                                               21
<210>  21
<211>  21
<212>  DNA
<213>  ˹
<220>  
<223>  GLI2
<400>  21
cgagggtcat ctggtggtaa t                                               21
<210>  22
<211>  19
<212>  DNA
<213>  ˹
<220>  
<223>   PTCH1
<400>  22
ccacagaagc gctcctaca                                                  19
<210>  23
<211>  19
<212>  DNA
<213>  ˹
<220>  
<223>   PTCH1
<400>  23
ctgtaatttc gccccttcc                                                  19
<210>  24
<211>  21
<212>  DNA
<213>  ˹
<220>  
<223>   GAPDH
<400>  24
tgacttcaac agcgacaccc a                                               21
<210>  25
<211>  21
<212>  DNA
<213>  ˹
<220>  
<223>   GAPDH
<400>  25
caccctgttg ctgtagccaa a                                               21


