﻿                      SEQUENCE LISTING

<110>  ZHEJIANG SENWEI BIOPHARMACEUTICAL DEVELOPMENT CO., LTD.

<120>  METHOD FOR RESCUING INFLUENZA VIRUS AND COMPOSITION THEREFOR

<130>  DSP1P191867XN

<150>  CN201910188724.6
<151>  2019-03-13

<160>  22

<170>  SIPOSequenceListing 1.0

<210>  1
<211>  2151
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimized PA gene of A/WSN/1933 of influenza virus H1N1  


<400>  1
atggaggact tcgtgaggca gtgcttcaac cccatgatcg tggagctggc cgagaaggcc        60
atgaaggagt acggcgagga cctgaagatc gagaccaaca agttcgccgc catctgcaca       120
cacctggagg tgtgcttcat gtactctgac ttccacttca tcgacgagca gggcgagtcc       180
atcgtggtgg agctggggga ccccaacgct ctgctgaagc accggttcga gatcatcgag       240
ggacgggaca ggaccatcgc ctggacagtg atcaacagca tctgcaacac cacaggggcc       300
gagaagccca agttcctgcc cgacctgtac gactacaaga agaacaggtt catcgagatc       360
ggcgtgacca ggagagaggt gcacatctac tacctggaga aggccaacaa gatcaagtcc       420
gagaagaccc acatccacat cttcagcttc acaggggagg agatggccac caaggccgac       480
tacacactgg acgaggagag cagggcccgg atcaagacca ggctgttcac aatcagacag       540
gagatggcct ccaggggcct gtgggactcc ttccggcaga gcgagagggg agaggagaca       600
atcgaggaga gattcgagat caccggcaca atgagaaagc tggccgacca gtccctgcca       660
cccaacttca gctctctgga gaagttcaga gcctacgtgg acgggttcga gcccaacggc       720
tacatcgagg ggaagctgag ccagatgtct aaggaggtga acgccagaat cgagcccttc       780
ctgaagagca cccccaggcc cctgagactg ccagacggac caccatgctc ccagcggagc       840
aagttcctgc tgatggacgc cctgaagctg tccatcgagg accccagcca cgagggagag       900
ggcatccccc tgtacgacgc catcaagtgc atgagaacat tcttcggctg gaaggagccc       960
aacgtggtga agccccacga gaaggggatc aaccccaact acctgctgag ctggaagcag      1020
gtgctggccg agctgcagga catcgagaac gaggagaaga tcccccggac caagaacatg      1080
aagaagacat ctcagctgaa gtgggctctg ggagagaaca tggctccaga gaaggtggac      1140
ttcgacgact gcaaggacgt gggggacctg aagcagtacg acagcgacga gcccgagctg      1200
aggtctctgg cctcctggat tcagaacgag ttcaacaagg cctgcgagct gaccgactcc      1260
agctggatcg agctggacga gatcggagag gacgctgctc ccatcgagca catcgccagc      1320
atgcggagga actacttcac cgccgaggtg tctcactgca gggccacaga gtacatcatg      1380
aagggcgtgt acatcaacac agccctgctg aacgcctcct gcgctgctat ggacgacttc      1440
cagctgatcc ccatgatcag caagtgcaga accaaggagg ggagacggaa gacaaacctg      1500
tacggcttca tcatcaaggg caggagccac ctgcggaacg acaccgacgt ggtgaacttc      1560
gtgtctatgg agttctccct gacagacccc cggctggagc cacacaagtg ggagaagtac      1620
tgcgtgctgg aggtcggcga catgctgctg aggtctgcca tcgggcacgt gtcccggccc      1680
atgttcctgt acgtgaggac caacggcaca agcaagatca agatgaagtg ggggatggag      1740
atgaggagat gcctgctgca gagcctgcag cagatcgagt ctatgatcga ggccgagtct      1800
tccgtgaagg agaaggacat gaccaaggag ttcttcgaga acaagtccga gacatggcca      1860
gtgggagaga gcccaaaggg agtggaggag ggctctatcg ggaaggtgtg ccggaccctg      1920
ctggccaaga gcgtgttcaa ctctctgtac gcctccccac agctggaggg cttcagcgcc      1980
gagtctagga agctgctgct gatcgtgcag gccctgagag acaacctgga gccagggacc      2040
ttcgacctgg gagggctgta cgaggccatc gaggagtgcc tgatcaacga cccctgggtg      2100
ctgctgaacg ccagctggtt caactctttc ctgacacacg ccctgagatg a               2151

<210>  2
<211>  2274
<212>  DNA
<213>  Artificial Sequence

<220>
<223> Codon-optimized PB1 gene of A/WSN/1933 of influenza virus H1N1 


<400>  2
atggacgtga accccacact gctgttcctg aaggtgcccg cccagaacgc catctccacc        60
acattcccct acaccggcga ccccccatac agccacggaa ccgggacagg ctacaccatg       120
gacacagtga acaggacaca ccagtactct gagagagggc ggtggaccac aaacaccgag       180
acaggagctc cacagctgaa ccccatcgac ggaccactgc cagaggacaa cgagccatcc       240
ggatacgctc agaccgactg cgtgctggag gccatggcct tcctggagga gagccacccc       300
gggatcttcg agacctcttg cctggagaca atggaggtgg tgcagcagac ccgggtggac       360
aagctgacac agggcaggca gacctacgac tggacactga acagaaacca gccagctgct       420
accgccctgg ccaacacaat cgaggtgttc agatccaacg gactgaccgc taacgagagc       480
ggccggctga tcgacttcct gaaggacgtg atggagtcta tgaacaagga ggagatggag       540
atcaccacac acttccagag aaagaggaga gtgcgggaca acatgacaaa gaagatggtg       600
acccagagaa caatcggcaa gagaaagcag cggctgaaca agaggagcta cctgatcaga       660
gccctgaccc tgaacaccat gacaaaggac gctgagagag ggaagctgaa gagaagggcc       720
atcgccaccc cagggatgca gatcaggggc ttcgtgtact tcgtggagac actggccagg       780
agcatctgcg agaagctgga gcagtccgga ctgccagtgg gagggaacga gaagaaggcc       840
aagctggcca acgtggtgcg gaagatgatg accaactctc aggacacaga gatcagcttc       900
accatcacag gggacaacac aaagtggaac gagaaccaga accccaggat gttcctggcc       960
atgatcacct acatcacacg gaaccagccc gagtggttca ggaacgtgct gtccatcgcc      1020
cccatcatgt tcagcaacaa gatggccaga ctggggaagg gctacatgtt cgagtctaag      1080
tccatgaaga tcagaaccca gatcccagct gagatgctgg ccagcatcga cctgaagtac      1140
ttcaacgact ctaccagaaa gaagatcgag aagatcagac cactgctgat cgacggaaca      1200
gccagcctgt ctcccgggat gatgatgggc atgttcaaca tgctgagcac cgtgctgggg      1260
gtgtctatcc tgaacctggg ccagaagagg cacacaaaga ccacatactg gtgggacggc      1320
ctgcagagct ctgacgactt cgccctgatc gtgaacgctc caaaccacga gggcatccag      1380
gctggcgtga acaggttcta cagaacatgc aagctgctgg ggatcaacat gagcaagaag      1440
aagagctaca tcaaccggac cggcacattc gagttcacct ctttcttcta ccgatacggg      1500
ttcgtggcca acttctccat ggagctgccc tctttcgggg tgtccggcat caacgagagc      1560
gccgacatgt ctatcggcgt gaccgtgatc aagaacaaca tgatcaacaa cgacctggga      1620
ccagctacag ctcagatggc cctgcagctg ttcatcaagg actacaggta cacctaccgg      1680
tgccacaggg gcgacaccca gatccagaca agacggagct tcgagatcaa gaagctgtgg      1740
gagcagaccc actctaaggc tggactgctg gtgtccgacg gaggacccaa cctgtacaac      1800
atcaggaacc tgcacatccc cgaggtgtgc ctgaagtggg agctgatgga cgaggactac      1860
cagggcagac tgtgcaaccc cctgaacccc ttcgtgaacc acaaggacat cgagtccgtg      1920
aacaacgccg tgatcatgcc agctcacgga ccagctaaga acatggagta cgacgccgtg      1980
gccaccacac acagctggat tcccaagagg aacagatcca tcctgaacac cagccagagg      2040
ggcatcctgg aggacgagca gatgtaccag aagtgctgca acctgttcga gaagttcttc      2100
ccctccagct cttacaggag acccgtgggc atctccagca tggtggaggc tatggtgagc      2160
cgggccagga tcgacgccag gatcgacttc gagtctggga gaatcaagaa ggaggagttc      2220
accgagatca tgaaaatctg cagcacaatc gaggagctgc ggaggcagaa gtga            2274

<210>  3
<211>  2280
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimized PB2 gene of A/WSN/1933 of influenza virus H1N1  


<400>  3
atggagagga tcaaggagct gagaaacctg atgtctcagt cccggacaag ggagatcctg        60
accaagacca cagtggacca catggccatc atcaagaagt acaccagcgg caggcaggag       120
aagaaccccg ccctgagaat gaagtggatg atggccatga agtaccccat cacagccgac       180
aagcggatca ccgagatgat ccccgagagg aatgagcagg gccagaccct gtggagcaag       240
atgaacgacg ccggcagcga cagagtgatg gtgagccccc tggccgtgac atggtggaac       300
agaaacggcc ccgtgacatc taccgtgcac taccccaaaa tctacaagac atacttcgag       360
aaggtggaga gactgaagca cggcaccttc gggccagtgc acttccggaa ccaggtgaag       420
atcaggagac gggtggacat caacccagga cacgccgacc tgtctgccaa ggaggcccag       480
gacgtgatca tggaggtggt gttccccaac gaagtgggcg ccagaatcct gaccagcgag       540
tctcagctga ccacaaccaa ggagaagaag gaggagctgc aggggtgcaa gatcagcccc       600
ctgatggtgg cctacatgct ggagcgggag ctggtgagaa agacccggtt cctgccagtg       660
gctggaggca ccagcagcgt gtacatcgag gtgctgcacc tgacacaggg cacctgctgg       720
gagcagatgt acacaccagg aggagaggct agaaacgacg acgtggacca gtccctgatc       780
atcgccgcca ggaacatcgt gaggagagct acagtgtctg ccgaccccct ggcctccctg       840
ctggagatgt gccacagcac ccagatcggc ggcatcagga tggtgaacat cctgaggcag       900
aaccccacag aggagcaggc cgtggacatc tgcaaggccg ccatgggcct gaggatctcc       960
agctctttct ccttcggcgg gttcacattc aagagaacca gcggctccag cgtgaagaga      1020
gaggaggagg tgctgacagg gaacctgcag accctgaaga tcagggtgca cgagggctac      1080
gaggagttca caatggtggg gcggagggct accgctatcc tgagaaaggc cacaagacgg      1140
ctgatccagc tgatcgtgag cggccgggac gagcagtcta tcgccgaggc catcatcgtg      1200
gccatggtgt tcagccagga ggactgcatg atcaaggccg tgagggggga cctgaacttc      1260
gtgaaccggg ccaaccagag gctgaacccc atgcaccagc tgctgagaca cttccagaag      1320
gacgccaagg tgctgttcca gaactggggc atcgagtcta tcgacaacgt gatgggcatg      1380
atcggcatcc tgcccgacat gacaccctcc accgagatga gcatgagggg cgtgagaatc      1440
tccaagatgg gggtggacga gtactcttcc gccgagaaga tcgtggtgag catcgacagg      1500
ttcctgagag tgcgggacca gagaggcaac gtgctgctgt ctcccgagga gatcagcgag      1560
acacagggga ccgagaagct gacaatcacc tacagctctt ccatgatgtg ggagatcaac      1620
ggccccgagt ctgtgctggt gaacacctac cagtggatca tcagaaactg ggagacagtg      1680
aagatccagt ggtcccagaa ccccaccatg ctgtacaaca agatggagtt cgagcccttc      1740
cagagcctgg tgccaaaggc cgtgaggggc cagtactctg ggttcgtgag aaccctgttc      1800
cagcagatgc gggacgtgct gggcacattc gacaccgccc agatcatcaa gctgctgccc      1860
ttcgccgccg ccccaccaaa gcagagccgg acacagttca gctctctgac catcaacgtg      1920
aggggctctg ggatgagaat cctggtgcgg gggaactccc ccgtgttcaa ctacaacaag      1980
acaaccaagc ggctgaccgt gctgggcaag gacgctggac cactgaccga ggaccccgac      2040
gagggcacag ccggggtgga gagcgccgtg ctgaggggct tcctgatcct ggggaaggag      2100
gacaggagat acggccccgc cctgagcatc aacgagctgt ctaacctggc caagggggag      2160
aaggccaacg tgctgatcgg ccagggcgac gtggtgctcg tgatgaagag gaagagaaac      2220
tccagcatcc tgaccgacag ccagacagcc accaagcgga tcaggatggc catcaactga      2280

<210>  4
<211>  4491
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimized NP gene of A/WSN/1933 of influenza virus H1N1


<400>  4
atggccacca agggcacaaa gaggagctac gagcagatgg agaccgacgg ggagaggcag        60
aacgccacag agatcagagc ctctgtgggc aagatgatcg acggcatcgg gagattctac       120
atccagatgt gcaccgagct gaagctgtcc gactacgagg ggagactgat ccagaactct       180
ctgacaatcg agcggatggt gctgtccgcc ttcgacgaga ggagaaacaa gtacctggag       240
gagcacccaa gcgccggcaa ggaccccaag aagaccggag gaccaatcta cagaagggtg       300
gacgggaagt ggagacggga gctgatcctg tacgacaagg aggagatcag gagaatctgg       360
aggcaggcta acaacggcga cgacgctacc gctgggctga cacacatgat gatctggcac       420
tctaacctga acgacgccac ctaccagagg acaagagccc tggtgcggac cggaatggac       480
cccaggatgt gctctctgat gcaggggtcc acactgccac ggaggagcgg agctgctgga       540
gctgccgtga agggcgtggg gaccatggtc atggagctga tccggatgat caagaggggc       600
atcaacgaca gaaacttctg gcggggcgag aacgggagac ggacaaggat cgcctacgag       660
agaatgtgca acatcctgaa gggcaagttc cagaccgccg cccagaggac aatggtggac       720
caggtgaggg agagcaggaa ccccggcaac gccgagttcg aggacctgat cttcctggct       780
agatccgccc tgatcctgag agggagcgtg gctcacaagt cttgcctgcc agcttgcgtg       840
tacggcagcg ccgtggcctc tgggtacgac ttcgagaggg agggctacag cctggtgggg       900
atcgacccct tcagactgct gcagaactcc caggtgtaca gcctgatcag accaaacgag       960
aacccagctc acaagagcca gctggtgtgg atggcctgcc actctgccgc cttcgaggac      1020
ctgagggtga gctctttcat cagaggaacc aaggtggtgc cccggggcaa gctgtccaca      1080
aggggggtgc agatcgccag caacgagaac atggagacca tggagtccag cacactggag      1140
ctgcggtcca ggtactgggc catcagaacc cggagcggcg ggaacacaaa ccagcagagg      1200
gcctcttccg gccagatcag catccagccc accttcagcg tgcagagaaa cctgcccttc      1260
gaccggccca caatcatggc tgccttcacc ggaaacacag agggacggac cagcgacatg      1320
aggacagaga tcatcagact gatggagagc gccagaccag aggacgtgtc tttccagggc      1380
aggggggtgt tcgagctgtc tgacgagaag gccacctccc ccatcgtgcc ctctttcgac      1440
atgtccaacg agggcagcta cttcttcggg gacaacgccg aggagtacga caactgaatg      1500
gccaccaagg gcacaaagag gagctacgag cagatggaga ccgacgggga gaggcagaac      1560
gccacagaga tcagagcctc tgtgggcaag atgatcgacg gcatcgggag attctacatc      1620
cagatgtgca ccgagctgaa gctgtccgac tacgagggga gactgatcca gaactctctg      1680
acaatcgagc ggatggtgct gtccgccttc gacgagagga gaaacaagta cctggaggag      1740
cacccaagcg ccggcaagga ccccaagaag accggaggac caatctacag aagggtggac      1800
gggaagtgga gacgggagct gatcctgtac gacaaggagg agatcaggag aatctggagg      1860
caggctaaca acggcgacga cgctaccgct gggctgacac acatgatgat ctggcactct      1920
aacctgaacg acgccaccta ccagaggaca agagccctgg tgcggaccgg aatggacccc      1980
aggatgtgct ctctgatgca ggggtccaca ctgccacgga ggagcggagc tgctggagct      2040
gccgtgaagg gcgtggggac catggtcatg gagctgatcc ggatgatcaa gaggggcatc      2100
aacgacagaa acttctggcg gggcgagaac gggagacgga caaggatcgc ctacgagaga      2160
atgtgcaaca tcctgaaggg caagttccag accgccgccc agaggacaat ggtggaccag      2220
gtgagggaga gcaggaaccc cggcaacgcc gagttcgagg acctgatctt cctggctaga      2280
tccgccctga tcctgagagg gagcgtggct cacaagtctt gcctgccagc ttgcgtgtac      2340
ggcagcgccg tggcctctgg gtacgacttc gagagggagg gctacagcct ggtggggatc      2400
gaccccttca gactgctgca gaactcccag gtgtacagcc tgatcagacc aaacgagaac      2460
ccagctcaca agagccagct ggtgtggatg gcctgccact ctgccgcctt cgaggacctg      2520
agggtgagct ctttcatcag aggaaccaag gtggtgcccc ggggcaagct gtccacaagg      2580
ggggtgcaga tcgccagcaa cgagaacatg gagaccatgg agtccagcac actggagctg      2640
cggtccaggt actgggccat cagaacccgg agcggcggga acacaaacca gcagagggcc      2700
tcttccggcc agatcagcat ccagcccacc ttcagcgtgc agagaaacct gcccttcgac      2760
cggcccacaa tcatggctgc cttcaccgga aacacagagg gacggaccag cgacatgagg      2820
acagagatca tcagactgat ggagagcgcc agaccagagg acgtgtcttt ccagggcagg      2880
ggggtgttcg agctgtctga cgagaaggcc acctccccca tcgtgccctc tttcgacatg      2940
tccaacgagg gcagctactt cttcggggac aacgccgagg agtacgacaa ctgaatggcc      3000
accaagggca caaagaggag ctacgagcag atggagaccg acggggagag gcagaacgcc      3060
acagagatca gagcctctgt gggcaagatg atcgacggca tcgggagatt ctacatccag      3120
atgtgcaccg agctgaagct gtccgactac gaggggagac tgatccagaa ctctctgaca      3180
atcgagcgga tggtgctgtc cgccttcgac gagaggagaa acaagtacct ggaggagcac      3240
ccaagcgccg gcaaggaccc caagaagacc ggaggaccaa tctacagaag ggtggacggg      3300
aagtggagac gggagctgat cctgtacgac aaggaggaga tcaggagaat ctggaggcag      3360
gctaacaacg gcgacgacgc taccgctggg ctgacacaca tgatgatctg gcactctaac      3420
ctgaacgacg ccacctacca gaggacaaga gccctggtgc ggaccggaat ggaccccagg      3480
atgtgctctc tgatgcaggg gtccacactg ccacggagga gcggagctgc tggagctgcc      3540
gtgaagggcg tggggaccat ggtcatggag ctgatccgga tgatcaagag gggcatcaac      3600
gacagaaact tctggcgggg cgagaacggg agacggacaa ggatcgccta cgagagaatg      3660
tgcaacatcc tgaagggcaa gttccagacc gccgcccaga ggacaatggt ggaccaggtg      3720
agggagagca ggaaccccgg caacgccgag ttcgaggacc tgatcttcct ggctagatcc      3780
gccctgatcc tgagagggag cgtggctcac aagtcttgcc tgccagcttg cgtgtacggc      3840
agcgccgtgg cctctgggta cgacttcgag agggagggct acagcctggt ggggatcgac      3900
cccttcagac tgctgcagaa ctcccaggtg tacagcctga tcagaccaaa cgagaaccca      3960
gctcacaaga gccagctggt gtggatggcc tgccactctg ccgccttcga ggacctgagg      4020
gtgagctctt tcatcagagg aaccaaggtg gtgccccggg gcaagctgtc cacaaggggg      4080
gtgcagatcg ccagcaacga gaacatggag accatggagt ccagcacact ggagctgcgg      4140
tccaggtact gggccatcag aacccggagc ggcgggaaca caaaccagca gagggcctct      4200
tccggccaga tcagcatcca gcccaccttc agcgtgcaga gaaacctgcc cttcgaccgg      4260
cccacaatca tggctgcctt caccggaaac acagagggac ggaccagcga catgaggaca      4320
gagatcatca gactgatgga gagcgccaga ccagaggacg tgtctttcca gggcaggggg      4380
gtgttcgagc tgtctgacga gaaggccacc tcccccatcg tgccctcttt cgacatgtcc      4440
aacgagggca gctacttctt cggggacaac gccgaggagt acgacaactg a               4491

<210>  5
<211>  9190
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pBudCE4.1


<400>  5
gcgcgcgttg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag        60
ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct       120
gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc       180
caatagggac tttccattga cgtcaatggg tggactattt acggtaaact gcccacttgg       240
cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat       300
ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca       360
tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc       420
gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga       480
gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat       540
tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctctggc       600
taactagaga acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga       660
cccaagcttg cattcctgca ggtcgacatc gatcttaagc agtacttcta gaggatccga       720
acaaaaactc atctcagaag aggatctgaa tatgcatacc ggtcatcatc accatcacca       780
ttgagtttga tccccgggaa ttcagacatg ataagataca ttgatgagtt tggacaaacc       840
acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta       900
tttgtaacca ttataagctg caataaacaa gttggggtgg gcgaagaact ccagcatgag       960
atccccgcgc tggaggatca tccagccggc gtcccggaaa acgattccga agcccaacct      1020
ttcatagaag gcggcggtgg aatcgaaatc tcgtagcacg tgtcagtcct gctcctcggc      1080
cacgaagtgc acgcagttgc cggccgggtc gcgcagggcg aactcccgcc cccacggctg      1140
ctcgccgatc tcggtcatgg ccggcccgga ggcgtcccgg aagttcgtgg acacgacctc      1200
cgaccactcg gcgtacagct cgtccaggcc gcgcacccac acccaggcca gggtgttgtc      1260
cggcaccacc tggtcctgga ccgcgctgat gaacagggtc acgtcgtccc ggaccacacc      1320
ggcgaagtcg tcctccacga agtcccggga gaacccgagc cggtcggtcc agaactcgac      1380
cgctccggcg acgtcgcgcg cggtgagcac cggaacggca ctggtcaact tggccatggt      1440
ttagttcctc accttgtcgt attatactat gccgatatac tatgccgatg attaattgtc      1500
aacacgtgct gatcagatcc gaaaatggat atacaagctc ccgggagctt tttgcaaaag      1560
cctaggcctc caaaaaagcc tcctcactac ttctggaata gctcagaggc agaggcggcc      1620
tcggcctctg cataaataaa aaaaattagt cagccatggg gcggagaatg ggcggaactg      1680
ggcggagtta ggggcgggat gggcggagtt aggggcggga ctatggttgc tgactaattg      1740
agatgcatgc tttgcatact tctgcctgct ggggagcctg gggactttcc acacctggtt      1800
gctgactaat tgagatgcat gctttgcata cttctgcctg ctggggagcc tggggacttt      1860
ccacaccctc gtcgagctag cttcgtgagg ctccggtgcc cgtcagtggg cagagcgcac      1920
atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgaaccg gtgcctagag      1980
aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga      2040
gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg      2100
gtttgccgcc agaacacagg taagtgccgt gtgtggttcc cgcgggcctg gcctctttac      2160
gggttatggc ccttgcgtgc cttgaattac ttccacctgg ctccagtacg tgattcttga      2220
tcccgagctg gagccagggg cgggccttgc gctttaggag ccccttcgcc tcgtgcttga      2280
gttgaggcct ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc      2340
tgtctcgctg ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg      2400
ctttttttct ggcaagatag tcttgtaaat gcgggccagg atctgcacac tggtatttcg      2460
gtttttgggc ccgcggccgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg      2520
cggggcctgc gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct      2580
gctctggtgc ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc      2640
cggtcggcac cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tccagggggc      2700
tcaaaatgga ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa      2760
agggcctttc cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc      2820
aggcacctcg attagttctg gagcttttgg agtacgtcgt ctttaggttg gggggagggg      2880
ttttatgcga tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg      2940
cacttgatgt aattctcgtt ggaatttgcc ctttttgagt ttggatcttg gttcattctc      3000
aagcctcaga cagtggttca aagttttttt cttccatttc aggtgtcgtg aacacgtggt      3060
cgcggccgct tcgaaggtac cagcacagtg gactcgagag atctggccgg ctgggcccgt      3120
ttcgaaggta agcctatccc taaccctctc ctcggtctcg attctacgcg taccggtcat      3180
catcaccatc accattgagt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc      3240
cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc      3300
actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct      3360
attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg      3420
catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag tggcggtaat      3480
acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca      3540
aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc      3600
tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata      3660
aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc      3720
gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc      3780
acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga      3840
accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc      3900
ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag      3960
gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag      4020
gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag      4080
ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca      4140
gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga      4200
cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgacattaa cctataaaaa      4260
taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg      4320
acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca      4380
agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc      4440
atcagagcag attgtactga gagtgcacca tatatgcggt gtgaaatacc gcacagatgc      4500
gtaaggagaa aataccgcat caggcgccat tcgccattca ggctgcgcaa ctgttgggaa      4560
gggcgatcgg tgcgggcctc ttcgctatta cgccagcgcg cgttgacatt gattattgac      4620
tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg      4680
cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt      4740
gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca      4800
atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc      4860
aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta      4920
catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac      4980
catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg      5040
atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg      5100
ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt      5160
acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca ctgcttactg      5220
gcttatcgaa attaatacga ctcactatag ggagacccaa gcttgcattc ctgcaggtcg      5280
acatcgatct taagcagtac ttctagagga tccgaacaaa aactcatctc agaagaggat      5340
ctgaatatgc ataccggtca tcatcaccat caccattgag tttgatcccc gggaattcag      5400
acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat      5460
gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata      5520
aacaagttgg ggtgggcgaa gaactccagc atgagatccc cgcgctggag gatcatccag      5580
ccggcgtccc ggaaaacgat tccgaagccc aacctttcat agaaggcggc ggtggaatcg      5640
aaatctcgta gcacgtgtca gtcctgctcc tcggccacga agtgcacgca gttgccggcc      5700
gggtcgcgca gggcgaactc ccgcccccac ggctgctcgc cgatctcggt catggccggc      5760
ccggaggcgt cccggaagtt cgtggacacg acctccgacc actcggcgta cagctcgtcc      5820
aggccgcgca cccacaccca ggccagggtg ttgtccggca ccacctggtc ctggaccgcg      5880
ctgatgaaca gggtcacgtc gtcccggacc acaccggcga agtcgtcctc cacgaagtcc      5940
cgggagaacc cgagccggtc ggtccagaac tcgaccgctc cggcgacgtc gcgcgcggtg      6000
agcaccggaa cggcactggt caacttggcc atggtttagt tcctcacctt gtcgtattat      6060
actatgccga tatactatgc cgatgattaa ttgtcaacac gtgctgatca gatccgaaaa      6120
tggatataca agctcccggg agctttttgc aaaagcctag gcctccaaaa aagcctcctc      6180
actacttctg gaatagctca gaggcagagg cggcctcggc ctctgcataa ataaaaaaaa      6240
ttagtcagcc atggggcgga gaatgggcgg aactgggcgg agttaggggc gggatgggcg      6300
gagttagggg cgggactatg gttgctgact aattgagatg catgctttgc atacttctgc      6360
ctgctgggga gcctggggac tttccacacc tggttgctga ctaattgaga tgcatgcttt      6420
gcatacttct gcctgctggg gagcctgggg actttccaca ccctcgtcga gctagcttcg      6480
tgaggctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg      6540
gggggagggg tcggcaattg aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa      6600
agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt      6660
gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acaggtaagt      6720
gccgtgtgtg gttcccgcgg gcctggcctc tttacgggtt atggcccttg cgtgccttga      6780
attacttcca cctggctcca gtacgtgatt cttgatcccg agctggagcc aggggcgggc      6840
cttgcgcttt aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg      6900
gccgccgcgt gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc      6960
tagccattta aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg      7020
taaatgcggg ccaggatctg cacactggta tttcggtttt tgggcccgcg gccggcgacg      7080
gggcccgtgc gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga      7140
gaatcggacg ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc      7200
cgtgtatcgc cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg      7260
aaagatggcc gcttcccggc cctgctccag ggggctcaaa atggaggacg cggcgctcgg      7320
gagagcgggc gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg      7380
cttcatgtga ctccacggag taccgggcgc cgtccaggca cctcgattag ttctggagct      7440
tttggagtac gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca      7500
ctgagtgggt ggagactgaa gttaggccag cttggcactt gatgtaattc tcgttggaat      7560
ttgccctttt tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt      7620
tttttcttcc atttcaggtg tcgtgaacac gtggtcgcgg ccgcttcgaa ggtaccagca      7680
cagtggactc gagagatctg gccggctggg cccgtttcga aggtaagcct atccctaacc      7740
ctctcctcgg tctcgattct acgcgtaccg gtcatcatca ccatcaccat tgagtttaaa      7800
cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc      7860
ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg      7920
aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg      7980
acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta      8040
tggcttctga ggcggaaaga accagtggcg gtaatacggt tatccacaga atcaggggat      8100
aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc      8160
gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc      8220
tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga      8280
agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt      8340
ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg      8400
taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc      8460
gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg      8520
gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc      8580
ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg      8640
ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc      8700
gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct      8760
caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt      8820
taagggattt tggtcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt      8880
ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc      8940
acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt      9000
gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg      9060
caccatatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc      9120
gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc      9180
tattacgcca                                                             9190

<210>  6
<211>  600
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Puro reporter gene 


<400>  6
atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc ccgggccgta        60
cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgacccggac       120
cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac       180
atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag       240
agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt       300
tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag       360
cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc       420
agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg       480
gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc       540
gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctag       600

<210>  7
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer


<400>  7
ggacctatat acaggagagt atagggaaag tggaggagag a                            41

<210>  8
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer


<400>  8
tctctcctcc actttcccta tactctcctg tatataggtc c                            41

<210>  9
<211>  50
<212>  DNA
<213>  Artificial Sequence 

<220>
<223>  Primer


<400>  9
gcccatccgg aagtctaagt ggctatggtg ttgatttcaa aaaaggttca                   50

<210>  10
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer


<400>  10
tgaacctttt ttgaaatcaa caccatagcc acttagactt ccggatgggc                   50

<210>  11
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer


<400>  11
ctgtcttcct gatgtgtact acttgattat ggccatatg                               39

<210>  12
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer


<400>  12
catatggcca taatcaagta gtacacatca ggaagacag                               39

<210>  13
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer


<400>  13
gtttgttgtc catcttccct attctgagta ctgatgtgtc ct                           42

<210>  14
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer


<400>  14
aggacacatc agtactcaga atagggaaga tggacaacaa ac                           42

<210>  15
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer


<400>  15
gatggagaac gccagaat                                                      18

<210>  16
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer


<400>  16
gcatcgtcac cattattagc                                                    20

<210>  17
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer


<400>  17
accaggctat tcaccataag                                                    20

<210>  18
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer


<400>  18
ccttccatcc aaagaatgtt c                                                  21

<210>  19
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer


<400>  19
ggacaggaac aggatacac                                                     19

<210>  20
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer


<400>  20
tgctgaacaa cctccatc                                                      18

<210>  21
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer


<400>  21
cgaaagcagg tcaattatat tca                                                23

<210>  22
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer


<400>  22
tacttgtcac tggtccattc                                                    20

