
                                SEQUENCE LISTING

<110> Qiagen Sciences, LLC
      Qiagen Manchester Ltd.
      Tong, Yanhong
      Lueerssen, Dietrich Wilhelm Karl
      Perroud, Thomas

<120> COMPOSITIONS AND METHODS FOR ADAPTOR DESIGN AND 
      NUCLEIC ACID LIBRARY CONSTRUCTION FOR ROLONY-
      BASED SEQUENCING

<130> 830109.417WO

<140> PCT
<141> 2020-03-02

<150> US 62/814,417
<151> 2019-03-06

<160> 36

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 27
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic Oligonucleotide/Adaptor sequence

<400> 1
ctcactcgtc acagcacctc ctccgct                                          27

<210> 2
<211> 17
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic Oligonucleotide/Adaptor sequence

<400> 2
gtacacgacg agccagt                                                     17

<210> 3
<211> 17
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic Oligonucleotide/Adaptor sequence

<400> 3
ctcacactca ccacgtc                                                     17

<210> 4
<211> 17
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic Oligonucleotide/Adaptor sequence

<220>
<221> modified_base  
<222> 1
<223> 5Phos

<400> 4
ctcacactca ccacgtc                                                     17

<210> 5
<211> 17
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic Oligonucleotide/Adaptor sequence

<400> 5
gtacacgacg agccagt                                                     17

<210> 6
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic Oligonucleotide/Adaptor sequence

<400> 6
tgagtgtgag gtacacgacg                                                  20

<210> 7
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic Oligonucleotide/Adaptor sequence

<400> 7
tgagtgtgag tgtacacgac g                                                21

<210> 8
<211> 17
<212> DNA
<213> Artificial Sequence

<220> 
<223> RCA primer

<400> 8
gtacacgacg agccagt                                                     17

<210> 9
<211> 26
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic oligonucleotide/adaptor sequence

<400> 9
ctcactcgtc acagcacctc ctccgc                                           26

<210> 10
<211> 26
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic oligonucleotide/adaptor sequence

<400> 10
ctcacactca ccacgtcggc tcgcag                                           26

<210> 11
<211> 27
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic oligonucleotide/adaptor sequence

<400> 11
ctcactcgtc acagcacctc ctccgct                                          27

<210> 12
<211> 17
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic oligonucleotide/adaptor sequence

<400> 12
gagtgtgagg tacacga                                                     17

<210> 13
<211> 17
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic oligonucleotide/adaptor sequence

<400> 13
accacgtcgg ctcgcag                                                     17

<210> 14
<211> 17
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic oligonucleotide/adaptor sequence

<220>
<221> modified_base  
<222> 1
<223> 5phos

<400> 14
accacgtcgg ctcgcag                                                     17

<210> 15
<211> 17
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic oligonucleotide/adaptor sequence

<400> 15
gagtgtgagg tacacga                                                     17

<210> 16
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic oligonucleotide/adaptor sequence

<400> 16
ccgacgtggt gagtgtgagg                                                  20

<210> 17
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic oligonucleotide/adaptor sequence

<400> 17
ccgacgtggt tgagtgtgag g                                                21

<210> 18
<211> 18
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic oligonucleotide/adaptor sequence

<400> 18
ggaggtgctg tgacgagt                                                    18

<210> 19
<211> 26
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic oligonucleotide/adaptor sequence

<400> 19
ctcactcgtc acagcacctc ctccgc                                           26

<210> 20
<211> 26
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic oligonucleotide/adaptor sequence

<400> 20
ctcacactca ccacgtcggc tcgcag                                           26

<210> 21
<211> 27
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic oligonucleotide/adaptor sequence

<400> 21
ctcactcgtc acagcacctc ctccgct                                          27

<210> 22
<211> 17
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic oligonucleotide/adaptor sequence

<400> 22
gagtgtgagg tacacga                                                     17

<210> 23
<211> 17
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic oligonucleotide/adaptor sequence

<400> 23
accacgtcgg ctcgcag                                                     17

<210> 24
<211> 17
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic oligonucleotide/adaptor sequence

<220>
<221> modified_base  
<222> 1
<223> 5Phos

<400> 24
accacgtcgg ctcgcag                                                     17

<210> 25
<211> 17
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic oligonucleotide/adaptor sequence

<400> 25
gagtgtgagg tacacga                                                     17

<210> 26
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic oligonucleotide/adaptor sequence

<400> 26
cctcacactc accacgtcgg                                                  20

<210> 27
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic oligonucleotide/adaptor sequence

<400> 27
cctcacactc taccacgtcg g                                                21

<210> 28
<211> 18
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic oligonucleotide/adaptor sequence

<400> 28
actcgtcaca gcacctcc                                                    18

<210> 29
<211> 26
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic oligonucleotide/adaptor sequence

<400> 29
gcggaggagg tgctgtgacg agtgag                                           26

<210> 30
<211> 26
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic oligonucleotide/adaptor sequence

<400> 30
ctgcgagccg acgtggtgag tgtgag                                           26

<210> 31
<211> 26
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic oligonucleotide/adaptor sequence

<400> 31
gagtgagcag tgtcgtggag gaggcg                                           26

<210> 32
<211> 26
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic oligonucleotide/adaptor sequence

<400> 32
gagtgagcag tgtcgtggag gaggcg                                           26

<210> 33
<211> 26
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic oligonucleotide/adaptor sequence

<400> 33
gagtgagcag tgtcgtggag gaggcg                                           26

<210> 34
<211> 40
<212> DNA
<213> Artificial Sequence

<220> 
<223> exemplary universal adaptor

<400> 34
gtaaaacgac ggccagtcaa gctatggaac accacgtcca                            40

<210> 35
<211> 40
<212> DNA
<213> Artificial Sequence

<220> 
<223> Exemplary universal adaptor

<400> 35
cattttgctg ccggtcagtt cgataccttg tggtgcaggt                            40

<210> 36
<211> 10
<212> DNA
<213> Artificial Sequence

<220> 
<223> Portion of a bridge oligonucleotide binding site

<400> 36
ctcacactca                                                             10
