                         SEQUENCE LISTING

<110>  Fred Hutchinson Cancer Research Center
 
<120>  PROTEIN RESIDUE MAPPING USING A COMBINATION OF DEEP MUTATIONAL 
       SCANNING AND PHAGE DISPLAY HIGH THROUGHPUT SEQUENCING

<130>  F053-0099PCT/19-113-US-PCT

<140>  PCT/US 2020/020429
<141>  2020-02-28

<150>  US 62/812,804
<151>  2019-03-01

<160>  96    

<170>  PatentIn version 3.5

<210>  1
<211>  67
<212>  PRT
<213>  Human immunodeficiency virus

<400>  1

Leu Thr Val Trp Gly Ile Lys Gln Leu Gln Ala Arg Ile Leu Ala Val 
1               5                   10                  15      


Glu Arg Tyr Leu Lys Asp Gln Gln Leu Leu Gly Ile Trp Gly Cys Ser 
            20                  25                  30          


Gly Lys Leu Ile Cys Thr Thr Ala Val Pro Trp Asn Ala Ser Trp Ser 
        35                  40                  45              


Asn Lys Ser Leu Glu Gln Ile Trp Asn His Thr Thr Trp Met Glu Trp 
    50                  55                  60                  


Asp Arg Glu 
65          


<210>  2
<211>  39
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Phage display peptides for QA255

<400>  2

Gln Gln Leu Leu Gly Ile Trp Gly Cys Ser Gly Lys Leu Ile Cys Thr 
1               5                   10                  15      


Thr Asn Val Pro Trp Asn Ser Ser Trp Ser Asn Lys Asn Gln Ser Glu 
            20                  25                  30          


Ile Trp Asp Arg Met Thr Trp 
        35                  


<210>  3
<211>  39
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Phage display peptides for QA255

<400>  3

Val Glu Arg Tyr Leu Arg Asp Gln Gln Leu Leu Gly Ile Trp Gly Cys 
1               5                   10                  15      


Ser Gly Lys Leu Ile Cys Thr Thr Asn Val Pro Trp Asn Ser Ser Trp 
            20                  25                  30          


Ser Asn Lys Ser Gln Asn Glu 
        35                  


<210>  4
<211>  39
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Phage display peptides for QA255

<400>  4

Glu Arg Tyr Leu Arg Asp Gln Gln Leu Leu Gly Ile Trp Gly Cys Ser 
1               5                   10                  15      


Gly Lys Leu Ile Cys Thr Thr Asn Val Pro Trp Asn Ser Ser Trp Ser 
            20                  25                  30          


Asn Arg Asn Leu Ser Glu Ile 
        35                  


<210>  5
<211>  39
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Phage display peptides for QA255

<400>  5

Lys Asp Gln Gln Leu Leu Gly Ile Trp Gly Cys Ser Gly Lys Leu Ile 
1               5                   10                  15      


Cys Thr Thr Asn Val Pro Trp Asn Ser Ser Trp Ser Asn Lys Ser Gln 
            20                  25                  30          


Asn Glu Ile Trp Asp Asn Met 
        35                  


<210>  6
<211>  39
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Phage display peptides for QA255

<400>  6

Leu Ala Leu Glu Arg Tyr Leu Gln Asp Gln Gln Leu Leu Gly Ile Trp 
1               5                   10                  15      


Gly Cys Ser Gly Lys Leu Ile Cys Ala Thr Thr Val Pro Trp Asn Ser 
            20                  25                  30          


Ser Trp Ser Asn Lys Thr Gln 
        35                  


<210>  7
<211>  39
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Phage display peptides for QA255

<400>  7

Asp Gln Gln Leu Leu Gly Ile Trp Gly Cys Ser Gly Lys Leu Ile Cys 
1               5                   10                  15      


Thr Thr Ala Val Pro Trp Asn Ser Ser Trp Ser Asn Lys Ser Gln Glu 
            20                  25                  30          


Asp Ile Trp Asp Asn Met Thr 
        35                  


<210>  8
<211>  39
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Phage display peptides for QA255

<400>  8

Gln Leu Leu Gly Ile Trp Gly Cys Ser Gly Lys Leu Ile Cys Thr Thr 
1               5                   10                  15      


Asn Val Pro Trp Asn Ser Ser Trp Ser Asn Lys Ser Leu Asp Glu Ile 
            20                  25                  30          


Trp Asn Asn Met Thr Trp Leu 
        35                  


<210>  9
<211>  39
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Phage display peptides for QA255

<400>  9

Leu Leu Gly Ile Trp Gly Cys Ser Gly Lys Leu Ile Cys Thr Thr Asn 
1               5                   10                  15      


Val Pro Trp Asn Ser Ser Trp Ser Asn Lys Ser Gln Asp Glu Ile Trp 
            20                  25                  30          


Gly Asn Met Thr Trp Leu Gln 
        35                  


<210>  10
<211>  39
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Phage display peptides for QA255

<400>  10

Arg Val Leu Ala Val Glu Arg Tyr Leu Lys Asp Gln Gln Leu Leu Gly 
1               5                   10                  15      


Ile Trp Gly Cys Ser Gly Lys Leu Ile Cys Thr Thr Asn Val Pro Trp 
            20                  25                  30          


Asn Ser Ser Trp Ser Asn Lys 
        35                  


<210>  11
<211>  39
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Phage display peptides for QA255

<400>  11

Val Leu Ala Ile Glu Arg Tyr Leu Gln Asp Gln Gln Leu Leu Gly Ile 
1               5                   10                  15      


Trp Gly Cys Ser Gly Lys Leu Ile Cys Thr Thr Ala Val Pro Trp Asn 
            20                  25                  30          


Ser Ser Trp Ser Asn Lys Ser 
        35                  


<210>  12
<211>  39
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Phage display peptides for QA255

<400>  12

Leu Gln Ala Arg Val Leu Ala Val Glu Arg Tyr Leu Lys Asp Gln Gln 
1               5                   10                  15      


Leu Leu Gly Ile Trp Gly Cys Ser Gly Lys Leu Ile Cys Thr Thr Ala 
            20                  25                  30          


Val Pro Trp Asn Ala Ser Trp 
        35                  


<210>  13
<211>  39
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Phage display peptides for QA255

<400>  13

Ala Arg Val Leu Ala Val Glu Arg Tyr Leu Lys Asp Gln Gln Leu Leu 
1               5                   10                  15      


Gly Ile Trp Gly Cys Ser Gly Lys Leu Ile Cys Thr Thr Ser Val Pro 
            20                  25                  30          


Trp Asn Ser Ser Trp Ser Asn 
        35                  


<210>  14
<211>  39
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Phage display peptides for QA255

<400>  14

Gln Leu Gln Ala Arg Val Leu Ala Val Glu Ser Tyr Leu Lys Asp Gln 
1               5                   10                  15      


Gln Leu Leu Gly Ile Trp Gly Cys Ser Gly Lys His Ile Cys Thr Thr 
            20                  25                  30          


Thr Val Pro Trp Asn Ser Ser 
        35                  


<210>  15
<211>  39
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Phage display peptides for QA255

<400>  15

Trp Gly Ile Lys Gln Leu Gln Ala Arg Val Leu Ala Val Glu Arg Tyr 
1               5                   10                  15      


Leu Arg Asp Gln Gln Leu Leu Gly Ile Trp Gly Cys Ser Gly Lys Leu 
            20                  25                  30          


Ile Cys Thr Thr Asn Val Pro 
        35                  


<210>  16
<211>  39
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Phage display peptides for QA255

<400>  16

Gly Ile Lys Gln Leu Gln Ala Arg Val Leu Ala Val Glu Arg Tyr Leu 
1               5                   10                  15      


Lys Asp Gln Gln Leu Leu Gly Ile Trp Gly Cys Ser Gly Lys Leu Ile 
            20                  25                  30          


Cys Thr Thr Asn Val Pro Trp 
        35                  


<210>  17
<211>  39
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Phage display peptides for QA255

<400>  17

Leu Thr Val Trp Gly Ile Lys Gln Leu Gln Ala Arg Val Leu Ala Val 
1               5                   10                  15      


Glu Arg Tyr Leu Lys Asp Gln Gln Leu Leu Gly Ile Trp Gly Cys Ser 
            20                  25                  30          


Gly Lys Leu Ile Cys Thr Thr 
        35                  


<210>  18
<211>  39
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Phage display peptides for QA255

<400>  18

Gln Ala Arg Ile Leu Ala Val Glu Arg Tyr Leu Lys Asp Gln Gln Leu 
1               5                   10                  15      


Leu Gly Ile Trp Gly Cys Ser Gly Lys His Ile Cys Thr Thr Thr Val 
            20                  25                  30          


Pro Trp Asn Ser Ser Trp Ser 
        35                  


<210>  19
<211>  39
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Phage display peptides for QA255

<400>  19

Val Trp Gly Ile Lys Gln Leu Gln Ala Arg Ile Leu Ala Val Glu Arg 
1               5                   10                  15      


Tyr Leu Lys Asp Gln Gln Leu Leu Gly Ile Trp Gly Cys Ser Gly Lys 
            20                  25                  30          


Leu Ile Cys Thr Thr Asn Val 
        35                  


<210>  20
<211>  39
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Phage display peptides for QA255

<400>  20

Thr Val Trp Gly Ile Lys Gln Leu Gln Thr Arg Val Leu Ala Ile Glu 
1               5                   10                  15      


Arg Tyr Leu Lys Asp Gln Gln Leu Leu Gly Ile Trp Gly Cys Ser Gly 
            20                  25                  30          


Lys Leu Ile Cys Thr Thr Ala 
        35                  


<210>  21
<211>  39
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Phage display peptides for QA255

<400>  21

Trp Gly Cys Ser Gly Lys Leu Ile Cys Thr Thr Ala Val Pro Trp Asn 
1               5                   10                  15      


Ala Ser Trp Ser Asn Lys Ser Leu Asp Glu Ile Trp Asp Asn Met Thr 
            20                  25                  30          


Trp Met Glu Trp Glu Arg Glu 
        35                  


<210>  22
<211>  39
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Phage display peptides for QA255

<400>  22

Ile Trp Gly Cys Ser Gly Lys His Ile Cys Thr Thr Thr Val Pro Trp 
1               5                   10                  15      


Asn Ser Ser Trp Ser Asn Lys Ser Leu Glu Glu Ile Trp Asn Asn Met 
            20                  25                  30          


Thr Trp Met Glu Trp Glu Arg 
        35                  


<210>  23
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  240-D

<400>  23

Trp Gly Cys Ser Gly Lys Leu Ile Cys Thr Thr Ala 
1               5                   10          


<210>  24
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  QA255.067

<400>  24

Leu Leu Gly Ile Trp Gly Cys Ser Gly Lys Leu Ile Cys Thr Thr 
1               5                   10                  15  


<210>  25
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  QA255.072

<400>  25

Trp Gly Cys Ser Gly Lys Leu Ile Cys Thr Thr Asn Val Pro 
1               5                   10                  


<210>  26
<211>  58
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  R2 forward primer


<220>
<221>  misc_feature
<222>  (30)..(30)
<223>  n = a, c, g, t/u, unknown, or other

<220>
<221>  misc_feature
<222>  (31)..(31)
<223>  n = a, c, g, t/u, unknown, or other

<220>
<221>  misc_feature
<222>  (32)..(32)
<223>  n = a, c, g, t/u, unknown, or other

<220>
<221>  misc_feature
<222>  (33)..(33)
<223>  n = a, c, g, t/u, unknown, or other

<220>
<221>  misc_feature
<222>  (34)..(34)
<223>  n = a, c, g, t/u, unknown, or other

<220>
<221>  misc_feature
<222>  (35)..(35)
<223>  n = a, c, g, t/u, unknown, or other

<220>
<221>  misc_feature
<222>  (36)..(36)
<223>  n = a, c, g, t/u, unknown, or other

<220>
<221>  misc_feature
<222>  (37)..(37)
<223>  n = a, c, g, t/u, unknown, or other

<400>  26
aatgatacgg cgaccaccga gatctacacn nnnnnnntcg tcggcagcgt ctccagtc         58


<210>  27
<211>  173
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  QA255 HIV envelope

<400>  27

Ala Val Gly Ile Gly Ala Val Phe Ile Gly Phe Leu Gly Ala Ala Gly 
1               5                   10                  15      


Ser Thr Met Gly Ala Ala Ser Ile Thr Leu Thr Val Gln Ala Arg Gln 
            20                  25                  30          


Leu Leu Ser Gly Ile Val Gln Gln Gln Ser Asn Leu Leu Arg Ala Ile 
        35                  40                  45              


Glu Ala Gln Gln Gln Met Leu Lys Leu Thr Val Trp Gly Ile Lys Gln 
    50                  55                  60                  


Leu Gln Ala Arg Val Leu Ala Val Glu Arg Tyr Leu Lys Asp Gln Gln 
65                  70                  75                  80  


Leu Leu Gly Ile Trp Gly Cys Ser Gly Lys Leu Ile Cys Thr Thr Ser 
                85                  90                  95      


Val Pro Trp Asn Ser Ser Trp Ser Asn Lys Thr Gln Ser Glu Ile Trp 
            100                 105                 110         


Asp Asn Met Thr Trp Leu Gln Trp Asp Lys Glu Ile Ser Asn Tyr Thr 
        115                 120                 125             


Gln Thr Ile Tyr Arg Leu Ile Glu Glu Ser Gln Ile Gln Gln Glu Lys 
    130                 135                 140                 


Asn Glu Gln Asp Leu Leu Ala Leu Asp Gln Trp Ala Ser Leu Trp Thr 
145                 150                 155                 160 


Trp Phe Asp Ile Ser Lys Trp Leu Trp Tyr Ile Arg Ile 
                165                 170             


<210>  28
<211>  173
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  QA255 HIV envelope

<400>  28

Ala Val Gly Ile Gly Ala Val Phe Ile Gly Phe Leu Gly Ala Ala Gly 
1               5                   10                  15      


Ser Thr Met Gly Ala Ala Ser Ile Thr Leu Thr Val Gln Ala Arg Gln 
            20                  25                  30          


Leu Leu Ser Gly Ile Val Gln Gln Gln Ser Asn Leu Leu Arg Ala Ile 
        35                  40                  45              


Glu Ala Gln Gln Gln Met Leu Lys Leu Thr Val Trp Gly Ile Lys Gln 
    50                  55                  60                  


Leu Gln Ala Arg Val Leu Ala Val Glu Arg Tyr Leu Lys Asp Gln Gln 
65                  70                  75                  80  


Leu Leu Gly Ile Trp Gly Cys Ser Gly Lys Leu Ile Cys Thr Thr Ser 
                85                  90                  95      


Val Pro Trp Asn Ser Ser Trp Ser Asn Lys Thr Gln Ser Glu Ile Trp 
            100                 105                 110         


Asp Asn Met Thr Trp Leu Gln Trp Asp Lys Glu Ile Ser Asn Tyr Thr 
        115                 120                 125             


Gln Thr Ile Tyr Lys Leu Ile Glu Glu Ser Gln Ile Gln Gln Glu Lys 
    130                 135                 140                 


Asn Glu Gln Asp Leu Leu Ala Leu Asp Gln Trp Ala Ser Leu Trp Thr 
145                 150                 155                 160 


Trp Phe Asp Ile Ser Lys Trp Leu Trp Tyr Ile Lys Ile 
                165                 170             


<210>  29
<211>  173
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  QA255 HIV envelope

<400>  29

Ala Val Gly Ile Gly Ala Val Phe Ile Gly Phe Leu Gly Ala Ala Gly 
1               5                   10                  15      


Ser Thr Met Gly Ala Ala Ser Ile Thr Leu Thr Val Gln Ala Arg Gln 
            20                  25                  30          


Leu Leu Ser Gly Ile Val Gln Gln Gln Ser Asn Leu Leu Arg Ala Ile 
        35                  40                  45              


Glu Ala Gln Gln His Met Leu Lys Leu Thr Val Trp Gly Ile Lys Gln 
    50                  55                  60                  


Leu Gln Ala Arg Val Leu Ala Val Glu Arg Tyr Leu Lys Asp Gln Gln 
65                  70                  75                  80  


Leu Leu Gly Ile Trp Gly Cys Ser Gly Lys Leu Ile Cys Thr Thr Ser 
                85                  90                  95      


Val Pro Trp Asn Ser Ser Trp Ser Asn Lys Thr Gln Ser Glu Ile Trp 
            100                 105                 110         


Asp Asn Met Thr Trp Leu Gln Trp Asp Lys Glu Ile Ser Asn Tyr Thr 
        115                 120                 125             


Gln Thr Ile Tyr Lys Leu Ile Glu Glu Ser Gln Ile Gln Gln Glu Lys 
    130                 135                 140                 


Asn Glu Gln Asp Leu Leu Ala Leu Asp Gln Trp Ala Ser Leu Trp Thr 
145                 150                 155                 160 


Trp Phe Asp Ile Ser Lys Trp Leu Trp Tyr Ile Arg Ile 
                165                 170             


<210>  30
<211>  173
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  QA255 HIV envelope

<400>  30

Ala Val Gly Leu Gly Ala Val Phe Phe Gly Phe Leu Gly Val Ala Gly 
1               5                   10                  15      


Ser Thr Met Gly Ala Ala Ser Ile Thr Leu Thr Val Gln Ala Arg Gln 
            20                  25                  30          


Leu Leu Ser Gly Ile Val Gln Gln Gln Ser Asn Leu Leu Arg Ala Ile 
        35                  40                  45              


Glu Ala Gln Gln Gln Met Leu Lys Leu Thr Val Trp Gly Ile Lys Gln 
    50                  55                  60                  


Leu Gln Ala Arg Val Leu Ala Val Glu Arg Tyr Leu Lys Asp Gln Gln 
65                  70                  75                  80  


Leu Leu Gly Ile Trp Gly Cys Ser Gly Lys Leu Ile Cys Thr Thr Ser 
                85                  90                  95      


Val Pro Trp Asn Ser Ser Trp Ser Asn Lys Thr Gln Ser Glu Ile Trp 
            100                 105                 110         


Asp Asn Met Thr Trp Leu Gln Trp Asp Lys Glu Ile Ser Asn Tyr Thr 
        115                 120                 125             


Gln Ile Ile Tyr Glu Leu Ile Glu Glu Ser Gln Ile Gln Gln Glu Lys 
    130                 135                 140                 


Asn Glu Gln Asp Leu Leu Ala Leu Asp Gln Trp Ala Ser Leu Trp Thr 
145                 150                 155                 160 


Trp Phe Asp Ile Ser Lys Trp Leu Trp Tyr Ile Arg Ile 
                165                 170             


<210>  31
<211>  173
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  QA255 HIV envelope

<400>  31

Ala Val Gly Ile Gly Ala Val Phe Ile Gly Phe Leu Gly Ala Ala Gly 
1               5                   10                  15      


Ser Thr Met Gly Ala Ala Ser Ile Thr Leu Thr Ala Gln Ala Arg Gln 
            20                  25                  30          


Leu Leu Ser Gly Ile Val Gln Gln Gln Ser Asn Leu Leu Arg Ala Ile 
        35                  40                  45              


Glu Ala Gln Gln Gln Met Leu Lys Leu Thr Val Trp Gly Ile Lys Gln 
    50                  55                  60                  


Leu Gln Ala Arg Val Leu Ala Val Glu Arg Tyr Leu Lys Asp Gln Gln 
65                  70                  75                  80  


Leu Leu Gly Ile Trp Gly Cys Ser Gly Lys Leu Ile Cys Thr Thr Ser 
                85                  90                  95      


Val Pro Trp Asn Ser Ser Trp Ser Asn Lys Thr Gln Ser Glu Ile Trp 
            100                 105                 110         


Asp Asn Met Thr Trp Leu Gln Trp Asp Lys Glu Ile Ser Asn Tyr Thr 
        115                 120                 125             


Gln Thr Ile Tyr Lys Leu Ile Glu Glu Ser Gln Ile Gln Gln Glu Lys 
    130                 135                 140                 


Asn Glu Gln Asp Leu Leu Ala Leu Asp Gln Trp Ala Ser Leu Trp Thr 
145                 150                 155                 160 


Trp Phe Asp Ile Ser Lys Trp Leu Trp Tyr Ile Lys Ile 
                165                 170             


<210>  32
<211>  173
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  QA255 HIV envelope

<400>  32

Ala Val Gly Leu Gly Ala Val Phe Phe Gly Phe Leu Gly Ala Ala Gly 
1               5                   10                  15      


Ser Thr Met Gly Ala Ala Ser Ile Thr Leu Thr Val Gln Ala Arg Gln 
            20                  25                  30          


Leu Leu Ser Gly Ile Val Gln Gln Gln Ser Asn Leu Leu Arg Ala Ile 
        35                  40                  45              


Glu Ala Gln Gln Gln Met Leu Lys Leu Thr Val Trp Gly Ile Lys Gln 
    50                  55                  60                  


Leu Gln Ala Arg Val Leu Ala Val Glu Arg Tyr Leu Lys Asp Gln Gln 
65                  70                  75                  80  


Leu Leu Gly Ile Trp Gly Cys Ser Gly Lys Leu Ile Cys Thr Thr Ser 
                85                  90                  95      


Val Pro Trp Asn Ser Ser Trp Ser Asn Lys Thr Glu Ser Glu Ile Trp 
            100                 105                 110         


Asp Asn Met Thr Trp Leu Gln Trp Asp Lys Glu Ile Ser Asn Tyr Thr 
        115                 120                 125             


Gln Thr Ile Tyr Glu Leu Leu Glu Lys Ser Gln Ile Gln Gln Glu Lys 
    130                 135                 140                 


Asn Glu Gln Asp Leu Leu Ala Leu Asp Gln Trp Ala Ser Leu Trp Thr 
145                 150                 155                 160 


Trp Phe Asp Ile Ser Lys Trp Leu Trp Tyr Ile Lys Ile 
                165                 170             


<210>  33
<211>  173
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  QA255 HIV envelope

<400>  33

Ala Val Gly Leu Gly Ala Val Phe Phe Gly Phe Leu Gly Ala Ala Gly 
1               5                   10                  15      


Ser Thr Met Gly Ala Ala Ser Ile Thr Leu Thr Val Gln Ala Arg Gln 
            20                  25                  30          


Leu Leu Ser Gly Ile Val Gln Gln Gln Ser Asn Leu Leu Arg Ala Ile 
        35                  40                  45              


Glu Ala Gln Gln Gln Met Leu Lys Leu Thr Val Trp Gly Ile Lys Gln 
    50                  55                  60                  


Leu Gln Ala Arg Val Leu Ala Val Glu Arg Tyr Leu Lys Asp Gln Gln 
65                  70                  75                  80  


Leu Leu Gly Ile Trp Gly Cys Ser Gly Lys Leu Ile Cys Thr Thr Ser 
                85                  90                  95      


Val Pro Trp Asn Ser Ser Trp Ser Asn Lys Thr Gln Ser Glu Ile Trp 
            100                 105                 110         


Asp Asn Met Thr Trp Leu Gln Trp Asp Lys Glu Ile Ser Asn Tyr Thr 
        115                 120                 125             


Gln Thr Ile Tyr Asn Leu Leu Glu Glu Ser Gln Ile Gln Gln Glu Lys 
    130                 135                 140                 


Asn Glu Gln Asp Leu Leu Ala Leu Asp Gln Trp Ala Ser Leu Trp Thr 
145                 150                 155                 160 


Trp Phe Asp Ile Ser Lys Trp Leu Trp Tyr Ile Lys Ile 
                165                 170             


<210>  34
<211>  173
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  QA255 HIV envelope

<400>  34

Ala Val Gly Leu Gly Ala Val Phe Phe Gly Phe Leu Gly Ala Ala Gly 
1               5                   10                  15      


Ser Thr Met Gly Ala Ala Ser Ile Thr Leu Thr Val Gln Ala Arg Gln 
            20                  25                  30          


Leu Leu Ser Gly Ile Val Gln Gln Gln Ser Asn Leu Leu Arg Ala Ile 
        35                  40                  45              


Glu Ala Gln Gln Gln Met Leu Lys Leu Thr Val Trp Gly Ile Lys Gln 
    50                  55                  60                  


Leu Gln Ala Arg Val Leu Ala Val Glu Arg Tyr Leu Lys Asp Gln Gln 
65                  70                  75                  80  


Leu Leu Gly Ile Trp Gly Cys Ser Gly Lys Leu Ile Cys Thr Thr Ser 
                85                  90                  95      


Val Pro Trp Asn Ser Ser Trp Ser Asn Lys Thr Gln Ser Glu Ile Trp 
            100                 105                 110         


Asp Asn Met Thr Trp Leu Gln Trp Asp Lys Glu Ile Ser Asn Tyr Thr 
        115                 120                 125             


Gln Thr Ile Tyr Glu Leu Leu Glu Glu Ser Gln Ile Gln Gln Glu Lys 
    130                 135                 140                 


Asn Glu Gln Asp Leu Leu Ala Leu Asp Gln Trp Ala Ser Leu Trp Thr 
145                 150                 155                 160 


Trp Phe Asp Ile Ser Lys Trp Leu Trp Tyr Ile Lys Ile 
                165                 170             


<210>  35
<211>  173
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  QA255 HIV envelope

<400>  35

Ala Val Gly Leu Gly Ala Val Phe Phe Gly Phe Leu Gly Ala Ala Gly 
1               5                   10                  15      


Ser Thr Met Gly Ala Ala Ser Val Thr Leu Thr Val Gln Ala Arg Gln 
            20                  25                  30          


Leu Leu Ser Gly Ile Val Gln Gln Gln Ser Asn Leu Leu Arg Ala Ile 
        35                  40                  45              


Glu Ala Gln Gln Gln Met Leu Lys Leu Thr Val Trp Gly Ile Lys Gln 
    50                  55                  60                  


Leu Gln Ala Arg Val Leu Ala Val Glu Arg Tyr Leu Lys Asp Gln Gln 
65                  70                  75                  80  


Leu Leu Gly Ile Trp Gly Cys Ser Gly Lys Leu Ile Cys Thr Thr Ser 
                85                  90                  95      


Val Pro Trp Asn Ser Ser Trp Ser Asn Lys Thr Gln Ser Glu Ile Trp 
            100                 105                 110         


Asp Asn Met Thr Trp Leu Gln Trp Asp Lys Glu Ile Ser Asn Tyr Thr 
        115                 120                 125             


Gln Thr Ile Tyr Glu Leu Leu Glu Glu Ser Gln Ile Gln Gln Glu Lys 
    130                 135                 140                 


Asn Glu Gln Asp Leu Leu Ala Leu Asp Gln Trp Ala Ser Leu Trp Thr 
145                 150                 155                 160 


Trp Phe Asp Ile Ser Lys Trp Leu Trp Tyr Ile Lys Ile 
                165                 170             


<210>  36
<211>  174
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  QA255 HIV envelope

<400>  36

Ala Val Val Gly Leu Gly Ala Val Phe Phe Gly Ser Leu Gly Ala Ala 
1               5                   10                  15      


Gly Ser Thr Met Gly Ala Ala Ser Ile Thr Leu Ala Val Gln Ala Arg 
            20                  25                  30          


Arg Leu Leu Ser Gly Ile Val Gln Gln Gln Ser Asn Leu Leu Arg Ala 
        35                  40                  45              


Ile Glu Ala Gln Gln Gln Met Leu Lys Leu Thr Val Trp Gly Ile Lys 
    50                  55                  60                  


Gln Leu Gln Ala Arg Val Leu Ala Val Glu Arg Tyr Leu Lys Asp Gln 
65                  70                  75                  80  


Gln Leu Leu Gly Ile Trp Gly Cys Ser Gly Lys Leu Ile Cys Thr Thr 
                85                  90                  95      


Ser Val Pro Trp Asn Ser Ser Trp Ser Asn Lys Thr Gln Ser Glu Ile 
            100                 105                 110         


Trp Asp Asn Met Thr Trp Leu Gln Trp Asp Lys Glu Ile Ser Asn Tyr 
        115                 120                 125             


Thr Gln Thr Ile Tyr Asn Leu Leu Glu Glu Ser Gln Ile Gln Gln Glu 
    130                 135                 140                 


Lys Asn Glu Gln Asp Leu Leu Ala Leu Asp Gln Trp Ala Ser Leu Trp 
145                 150                 155                 160 


Thr Trp Phe Asp Ile Ser Lys Trp Leu Trp Tyr Ile Lys Ile 
                165                 170                 


<210>  37
<211>  173
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  QA255 HIV envelope

<400>  37

Ala Val Gly Leu Gly Ala Val Phe Phe Gly Phe Leu Gly Ala Ala Gly 
1               5                   10                  15      


Ser Thr Met Gly Ala Ala Ser Ile Thr Leu Thr Val Gln Ala Arg Gln 
            20                  25                  30          


Leu Leu Ser Gly Ile Val Gln Gln Gln Ser Asn Leu Leu Arg Ala Ile 
        35                  40                  45              


Glu Ala Gln Gln Gln Met Leu Lys Leu Thr Val Trp Gly Ile Lys Gln 
    50                  55                  60                  


Leu Gln Ala Arg Val Leu Ala Val Glu Arg Tyr Leu Lys Asp Gln Gln 
65                  70                  75                  80  


Leu Leu Gly Ile Trp Gly Cys Ser Gly Lys Leu Ile Cys Thr Thr Ser 
                85                  90                  95      


Val Pro Trp Asn Ser Ser Trp Ser Asn Lys Thr Gln Ser Glu Ile Trp 
            100                 105                 110         


Asp Asn Met Thr Trp Leu Gln Trp Asp Lys Glu Ile Ser Asn Tyr Thr 
        115                 120                 125             


Gln Thr Ile Tyr Glu Leu Leu Glu Lys Ser Gln Ile Gln Gln Glu Lys 
    130                 135                 140                 


Asn Glu Gln Asp Leu Leu Ala Leu Asp Gln Trp Ala Ser Leu Trp Thr 
145                 150                 155                 160 


Trp Phe Asp Ile Ser Lys Trp Leu Trp Tyr Ile Arg Ile 
                165                 170             


<210>  38
<211>  173
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  QA255 HIV envelope

<400>  38

Ala Val Gly Ile Gly Ala Met Phe Ile Gly Phe Leu Gly Ala Ala Gly 
1               5                   10                  15      


Ser Thr Met Gly Ala Ala Ser Ile Thr Leu Thr Val Gln Ala Arg Gln 
            20                  25                  30          


Leu Leu Ser Gly Ile Val Gln Gln Gln Ser Asn Leu Leu Arg Ala Ile 
        35                  40                  45              


Glu Ala Gln Gln Gln Met Leu Lys Leu Thr Val Trp Gly Ile Lys Gln 
    50                  55                  60                  


Leu Gln Ala Arg Val Leu Ala Val Glu Arg Tyr Leu Lys Asp Gln Gln 
65                  70                  75                  80  


Leu Leu Gly Ile Trp Gly Cys Ser Gly Lys Leu Ile Cys Thr Thr Ser 
                85                  90                  95      


Val Pro Trp Asn Ser Ser Trp Ser Asn Lys Thr Gln Ser Glu Ile Trp 
            100                 105                 110         


Asp Asn Met Thr Trp Leu Gln Trp Asp Lys Glu Ile Ser Asn Tyr Thr 
        115                 120                 125             


Gln Thr Ile Tyr Lys Leu Ile Glu Glu Ser Gln Ile Gln Gln Glu Lys 
    130                 135                 140                 


Asn Glu Gln Asp Leu Leu Ala Leu Asp Gln Trp Ala Ser Leu Trp Thr 
145                 150                 155                 160 


Trp Phe Asp Ile Ser Lys Trp Leu Trp Tyr Ile Lys Ile 
                165                 170             


<210>  39
<211>  173
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  QA255 HIV envelope

<400>  39

Ala Val Gly Leu Gly Ala Leu Phe Phe Gly Phe Leu Gly Ala Ala Gly 
1               5                   10                  15      


Ser Thr Met Gly Ala Ala Ser Ile Thr Leu Thr Val Gln Ala Arg Gln 
            20                  25                  30          


Leu Leu Ser Gly Ile Val Gln Gln Gln Ser Asn Leu Leu Arg Ala Ile 
        35                  40                  45              


Glu Ala Gln Gln Gln Met Leu Lys Leu Thr Val Trp Gly Ile Lys Gln 
    50                  55                  60                  


Leu Gln Ala Arg Val Leu Ala Val Glu Arg Tyr Leu Lys Asp Gln Gln 
65                  70                  75                  80  


Leu Leu Gly Ile Trp Gly Cys Ser Gly Lys Leu Ile Cys Thr Thr Ser 
                85                  90                  95      


Val Pro Trp Asn Ser Ser Trp Ser Asn Lys Thr Gln Ser Glu Ile Trp 
            100                 105                 110         


Asp Asn Met Thr Trp Leu Gln Trp Asp Lys Glu Ile Ser Asn Tyr Thr 
        115                 120                 125             


Gln Thr Ile Tyr Asn Leu Leu Glu Glu Ser Gln Ile Gln Gln Glu Lys 
    130                 135                 140                 


Asn Glu Lys Asp Leu Leu Ala Leu Asp Gln Trp Ala Ser Leu Trp Thr 
145                 150                 155                 160 


Trp Phe Asp Ile Ser Lys Trp Leu Trp Tyr Ile Lys Thr 
                165                 170             


<210>  40
<211>  173
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  QA255 HIV envelope

<400>  40

Ala Ile Gly Leu Gly Ala Leu Phe Phe Gly Phe Leu Gly Ala Ala Gly 
1               5                   10                  15      


Ser Thr Met Gly Ala Ala Ser Ile Thr Leu Thr Val Gln Ala Arg Gln 
            20                  25                  30          


Leu Leu Ser Gly Ile Val Gln Gln Gln Ser Asn Leu Leu Arg Ala Ile 
        35                  40                  45              


Glu Ala Gln Gln Gln Met Leu Lys Leu Thr Val Trp Gly Ile Lys Arg 
    50                  55                  60                  


Leu Gln Ala Arg Val Leu Ala Val Glu Arg Tyr Leu Lys Asp Gln Gln 
65                  70                  75                  80  


Leu Leu Gly Ile Trp Gly Cys Ser Gly Lys Leu Ile Cys Thr Thr Ser 
                85                  90                  95      


Val Pro Trp Asn Ser Ser Trp Ser Asn Lys Thr Gln Lys Glu Ile Trp 
            100                 105                 110         


Asp Asn Met Thr Trp Leu Gln Trp Asp Lys Glu Ile Ser Asn Tyr Thr 
        115                 120                 125             


Gln Thr Ile Tyr Asn Leu Leu Glu Glu Ser Gln Ile Gln Gln Glu Lys 
    130                 135                 140                 


Asn Glu Lys Asp Leu Leu Ala Leu Asp Gln Trp Ala Ser Leu Trp Thr 
145                 150                 155                 160 


Trp Phe Asp Ile Ser Asn Trp Leu Trp Tyr Ile Lys Ile 
                165                 170             


<210>  41
<211>  173
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  QA255 HIV envelope

<400>  41

Ala Ile Gly Leu Gly Ala Leu Phe Phe Gly Phe Leu Gly Ala Ala Gly 
1               5                   10                  15      


Ser Thr Met Gly Ala Ala Ser Ile Thr Leu Thr Val Gln Ala Arg Gln 
            20                  25                  30          


Leu Leu Ser Gly Ile Val Gln Gln Gln Ser Asn Leu Leu Arg Ala Ile 
        35                  40                  45              


Glu Ala Gln Gln Gln Met Leu Lys Leu Thr Val Trp Gly Ile Lys Gln 
    50                  55                  60                  


Leu Gln Ala Arg Val Leu Ala Val Glu Arg Tyr Leu Lys Asp Gln Gln 
65                  70                  75                  80  


Leu Leu Gly Ile Trp Gly Cys Ser Gly Lys Leu Ile Cys Thr Thr Ser 
                85                  90                  95      


Val Pro Trp Asn Ser Ser Trp Ser Asn Lys Thr Gln Ser Glu Ile Trp 
            100                 105                 110         


Asp Asn Met Thr Trp Leu Gln Trp Asp Lys Glu Ile Ser Asn Tyr Thr 
        115                 120                 125             


Gln Thr Ile Tyr Asn Leu Leu Glu Glu Ser Gln Ile Gln Gln Glu Lys 
    130                 135                 140                 


Asn Glu Lys Asp Leu Leu Ala Leu Asp Gln Trp Ala Ser Leu Trp Thr 
145                 150                 155                 160 


Trp Phe Asp Ile Ser Asn Trp Leu Trp Tyr Ile Lys Ile 
                165                 170             


<210>  42
<211>  173
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  QA255 HIV envelope

<400>  42

Ala Ile Gly Leu Gly Ala Leu Phe Phe Gly Phe Leu Gly Ala Ala Gly 
1               5                   10                  15      


Ser Thr Met Gly Ala Ala Ser Ile Thr Leu Thr Val Gln Ala Arg Gln 
            20                  25                  30          


Leu Leu Ser Gly Ile Val Gln Gln Gln Ser Asn Leu Leu Arg Ala Ile 
        35                  40                  45              


Glu Ala Gln Gln Gln Met Leu Lys Leu Thr Val Trp Gly Ile Lys Gln 
    50                  55                  60                  


Leu Gln Ala Arg Val Leu Ala Val Glu Arg Tyr Leu Lys Asp Gln Gln 
65                  70                  75                  80  


Leu Leu Gly Ile Trp Gly Cys Ser Gly Lys Leu Ile Cys Thr Thr Ser 
                85                  90                  95      


Val Pro Trp Asn Ser Ser Trp Ser Asn Lys Thr Gln Arg Glu Ile Trp 
            100                 105                 110         


Asp Asn Met Thr Trp Leu Gln Trp Asp Lys Glu Ile Ser Asn Tyr Thr 
        115                 120                 125             


Gln Thr Ile Tyr Asn Leu Leu Glu Glu Ser Gln Ile Gln Gln Glu Lys 
    130                 135                 140                 


Asn Glu Lys Asp Leu Leu Ala Leu Asp Gln Trp Ala Ser Leu Trp Thr 
145                 150                 155                 160 


Trp Phe Asn Ile Ser Asn Trp Leu Trp Tyr Ile Lys Ile 
                165                 170             


<210>  43
<211>  173
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  QA255 HIV envelope

<400>  43

Ala Ile Gly Leu Gly Ala Leu Phe Phe Gly Phe Leu Gly Ala Ala Gly 
1               5                   10                  15      


Ser Thr Met Gly Ala Ala Ser Ile Thr Leu Thr Val Gln Ala Arg Gln 
            20                  25                  30          


Leu Leu Ser Gly Ile Val Gln Gln Gln Ser Asn Leu Leu Arg Ala Ile 
        35                  40                  45              


Glu Ala Gln Gln Gln Met Leu Lys Leu Thr Val Trp Gly Ile Lys Gln 
    50                  55                  60                  


Leu Gln Ala Arg Val Leu Ala Val Glu Arg Tyr Leu Lys Asp Gln Gln 
65                  70                  75                  80  


Leu Leu Gly Ile Trp Gly Cys Ser Gly Lys Leu Ile Cys Thr Thr Ser 
                85                  90                  95      


Val Pro Trp Asn Ser Ser Trp Ser Asn Lys Thr Gln Gly Glu Ile Trp 
            100                 105                 110         


Asp Asn Met Thr Trp Leu Gln Trp Asp Lys Glu Ile Ser Asn Tyr Thr 
        115                 120                 125             


Gln Ile Ile Tyr Glu Leu Leu Glu Glu Ser Gln Thr Gln Gln Glu Lys 
    130                 135                 140                 


Asn Glu Lys Asp Leu Leu Ala Leu Asp Gln Trp Ala Ser Leu Trp Thr 
145                 150                 155                 160 


Trp Phe Asp Ile Ser Asn Trp Leu Trp Tyr Ile Lys Ile 
                165                 170             


<210>  44
<211>  178
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  QA255 HIV envelope

<400>  44

Ala Ile Gly Leu Gly Ala Leu Phe Phe Gly Phe Leu Gly Val Gly Phe 
1               5                   10                  15      


Leu Gly Ala Ala Gly Ser Thr Met Gly Ala Ala Ser Ile Thr Leu Thr 
            20                  25                  30          


Val Gln Ala Arg Gln Leu Leu Ser Gly Ile Val Gln Gln Gln Ser Asn 
        35                  40                  45              


Leu Leu Arg Ala Ile Glu Ala Gln Gln Gln Met Leu Lys Leu Thr Val 
    50                  55                  60                  


Trp Gly Ile Lys Gln Leu Gln Ala Arg Val Leu Ala Val Glu Arg Tyr 
65                  70                  75                  80  


Leu Lys Asp Gln Gln Leu Leu Gly Ile Trp Gly Cys Ser Gly Lys Leu 
                85                  90                  95      


Ile Cys Thr Thr Ser Val Pro Trp Asn Ser Ser Trp Ser Asn Lys Thr 
            100                 105                 110         


Gln Lys Glu Ile Trp Asp Asn Met Thr Trp Leu Gln Trp Asp Lys Glu 
        115                 120                 125             


Ile Ser Asn Tyr Thr Gln Thr Ile Tyr Asn Leu Leu Glu Glu Ser Gln 
    130                 135                 140                 


Ile Gln Gln Glu Lys Asn Glu Lys Asp Leu Leu Ala Leu Asp Gln Trp 
145                 150                 155                 160 


Ala Ser Leu Trp Thr Trp Phe Asn Ile Ser Asn Trp Leu Trp Tyr Ile 
                165                 170                 175     


Lys Ile 
        


<210>  45
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  known epitope for 240D

<400>  45

Leu Leu Gly Ile Trp Gly Cys Ser Gly 
1               5                   


<210>  46
<211>  66
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  R2 reverse primer


<220>
<221>  misc_feature
<222>  (25)..(25)
<223>  n = a, c, g, t/u, unknown, or other

<220>
<221>  misc_feature
<222>  (26)..(26)
<223>  n = a, c, g, t/u, unknown, or other

<220>
<221>  misc_feature
<222>  (27)..(27)
<223>  n = a, c, g, t/u, unknown, or other

<220>
<221>  misc_feature
<222>  (28)..(28)
<223>  n = a, c, g, t/u, unknown, or other

<220>
<221>  misc_feature
<222>  (29)..(29)
<223>  n = a, c, g, t/u, unknown, or other

<220>
<221>  misc_feature
<222>  (30)..(30)
<223>  n = a, c, g, t/u, unknown, or other

<220>
<221>  misc_feature
<222>  (31)..(31)
<223>  n = a, c, g, t/u, unknown, or other

<220>
<221>  misc_feature
<222>  (32)..(32)
<223>  n = a, c, g, t/u, unknown, or other

<400>  46
caagcagaag acggcatacg agatnnnnnn nngtctcgtg ggctcggaga tgtgtataag       60

agacag                                                                  66


<210>  47
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide with tolerated mutation

<400>  47

Leu Leu Gly Ile Trp Thr Cys Ser Gly 
1               5                   


<210>  48
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide with tolerated mutation

<400>  48

Leu Ile Gly Ile Trp Gly Cys Ser Gly 
1               5                   


<210>  49
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide with tolerated mutation

<400>  49

Leu Leu Gly Ile Trp Gly Cys Thr Gly 
1               5                   


<210>  50
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide with mutation that disrupts epitope

<400>  50

Leu Leu Ser Ile Trp Gly Cys Ser Gly 
1               5                   


<210>  51
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide with mutation that disrupts epitope

<400>  51

Leu Leu Gly Ile Trp Gly Thr Ser Gly 
1               5                   


<210>  52
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide with mutation that disrupts epitope

<400>  52

Leu Pro Gly Ile Trp Gly Cys Ser Gly 
1               5                   


<210>  53
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide with mutation that disrupts epitope

<400>  53

Leu Leu Gly Ile Ala Gly Cys Ser Gly 
1               5                   


<210>  54
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Gp41 peptides strain B41

<400>  54

Arg Lys Ser Ile His Ile Gly Pro Gly Arg Ala Phe Tyr 
1               5                   10              


<210>  55
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  V3 peptides strain B41

<400>  55

Glu Lys Ser Ile His Ile Gly Pro Gly Arg Ala Phe Tyr 
1               5                   10              


<210>  56
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  V3 peptides strain B41

<400>  56

Arg Thr Ser Ile His Ile Gly Pro Gly Arg Ala Phe Tyr 
1               5                   10              


<210>  57
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  V3 peptides strain B41

<400>  57

Arg Lys Ser Ile His Ile Gly Pro Gly Trp Ala Phe Tyr 
1               5                   10              


<210>  58
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  V3 peptides strain B41

<400>  58

Arg Lys Ser Ile His Ile Gly Pro Gly Arg Ala Thr Tyr 
1               5                   10              


<210>  59
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  V3 peptides strain B41

<400>  59

Arg Lys Ser Ile His Ile Gly Pro Gly Arg Ala Phe Lys 
1               5                   10              


<210>  60
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  V3 peptides strain B41

<400>  60

Arg Lys Ser Ile His Trp Gly Pro Gly Arg Ala Phe Tyr 
1               5                   10              


<210>  61
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  V3 peptides strain B41

<400>  61

Leu Leu Gly Ile Trp Gly Cys Ser Gly Lys Leu Ile Cys Thr Thr Asn 
1               5                   10                  15      


Val Pro 
        


<210>  62
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  V3 peptides strain B41

<400>  62

Leu Leu Gly Ile Trp Gly Asp Ser Gly Lys Leu Ile Cys Thr Thr Asn 
1               5                   10                  15      


Val Pro 
        


<210>  63
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  V3 peptides strain B41

<400>  63

Leu Lys Gly Ile Trp Gly Cys Ser Gly Lys Leu Ile Cys Thr Thr Asn 
1               5                   10                  15      


Val Pro 
        


<210>  64
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  V3 peptides strain B41

<400>  64

Leu Leu Gly Ile Trp Gly Cys Lys Gly Lys Leu Ile Cys Thr Thr Asn 
1               5                   10                  15      


Val Pro 
        


<210>  65
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  V3 peptides strain B41

<400>  65

Leu Leu Gly Ile Trp Gly Cys Ser Gly Lys Glu Ile Cys Thr Thr Asn 
1               5                   10                  15      


Val Pro 
        


<210>  66
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  His tag

<400>  66

His His His His His His 
1               5       


<210>  67
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Flaag tag

<400>  67

Asp Tyr Lys Asp Asp Asp Asp Lys 
1               5               


<210>  68
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Xpress tag

<400>  68

Asp Leu Tyr Asp Asp Asp Asp Lys 
1               5               


<210>  69
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Avi tag

<400>  69

Gly Leu Asn Asp Ile Phe Glu Ala Gln Lys Ile Glu Trp His Glu 
1               5                   10                  15  


<210>  70
<211>  26
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Calmodulin tag

<400>  70

Lys Arg Arg Trp Lys Lys Asn Phe Ile Ala Val Ser Ala Ala Asn Arg 
1               5                   10                  15      


Phe Lys Lys Ile Ser Ser Ser Gly Ala Leu 
            20                  25      


<210>  71
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HA tag

<400>  71

Tyr Pro Tyr Asp Val Pro Asp Tyr Ala 
1               5                   


<210>  72
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Myc tag

<400>  72

Glu Gln Lys Leu Ile Ser Glu Glu Asp Leu 
1               5                   10  


<210>  73
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Strep tag

<400>  73

Trp Arg His Pro Gln Phe Gly Gly 
1               5               


<210>  74
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Gly-Ser linker

<400>  74

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10                  15  


<210>  75
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Buffer Sequence


<220>
<221>  MISC_FEATURE
<222>  (2)..(10)
<223>  The amino acids can either be present or absent

<400>  75

Gly Gly Gly Gly Gly Gly Gly Gly Gly Gly 
1               5                   10  


<210>  76
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Buffer Sequence


<220>
<221>  MISC_FEATURE
<222>  (2)..(10)
<223>  The amino acids can either be present or absent

<400>  76

Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser 
1               5                   10  


<210>  77
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Buffer Sequence


<220>
<221>  MISC_FEATURE
<222>  (2)..(10)
<223>  The amino acids can either be present or absent

<400>  77

Ala Ala Ala Ala Ala Ala Ala Ala Ala Ala 
1               5                   10  


<210>  78
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Buffer Sequence


<220>
<221>  MISC_FEATURE
<222>  (3)..(20)
<223>  (GlySer) can either be present or absent

<400>  78

Gly Ser Gly Ser Gly Ser Gly Ser Gly Ser Gly Ser Gly Ser Gly Ser 
1               5                   10                  15      


Gly Ser Gly Ser 
            20  


<210>  79
<211>  40
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Buffer Sequence


<220>
<221>  MISC_FEATURE
<222>  (5)..(40)
<223>  (GlySerSerGly) can either be present or absent

<400>  79

Gly Ser Ser Gly Gly Ser Ser Gly Gly Ser Ser Gly Gly Ser Ser Gly 
1               5                   10                  15      


Gly Ser Ser Gly Gly Ser Ser Gly Gly Ser Ser Gly Gly Ser Ser Gly 
            20                  25                  30          


Gly Ser Ser Gly Gly Ser Ser Gly 
        35                  40  


<210>  80
<211>  30
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Buffer Sequence


<220>
<221>  MISC_FEATURE
<222>  (4)..(30)
<223>  (GlySerGly) can either be present or absent

<400>  80

Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly 
1               5                   10                  15      


Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly 
            20                  25                  30  


<210>  81
<211>  30
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Buffer Sequence


<220>
<221>  MISC_FEATURE
<222>  (4)..(30)
<223>  (GlySerSer) can either be present or absent

<400>  81

Gly Ser Ser Gly Ser Ser Gly Ser Ser Gly Ser Ser Gly Ser Ser Gly 
1               5                   10                  15      


Ser Ser Gly Ser Ser Gly Ser Ser Gly Ser Ser Gly Ser Ser 
            20                  25                  30  


<210>  82
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Buffer Sequence


<220>
<221>  MISC_FEATURE
<222>  (3)..(20)
<223>  (GlyAla) can either be present or absent

<400>  82

Gly Ala Gly Ala Gly Ala Gly Ala Gly Ala Gly Ala Gly Ala Gly Ala 
1               5                   10                  15      


Gly Ala Gly Ala 
            20  


<210>  83
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  STREP tag II

<400>  83

Trp Ser His Pro Gln Phe Glu Lys 
1               5               


<210>  84
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Softag 1

<400>  84

Ser Leu Ala Glu Leu Leu Asn Ala Gly Leu Gly Gly Ser 
1               5                   10              


<210>  85
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Softag 3

<400>  85

Thr Gln Asp Pro Ser Arg Val Gly 
1               5               


<210>  86
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  V5 tag

<400>  86

Gly Lys Pro Ile Pro Asn Pro Leu Leu Gly Leu Asp Ser Thr 
1               5                   10                  


<210>  87
<211>  58
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  adapter sequence

<400>  87
aatgatacgg cgaccaccga gatctacact ctttccctac acgacgctct tccgatct         58


<210>  88
<211>  63
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  adapter sequence


<220>
<221>  misc_feature
<222>  (34)..(39)
<223>  n is a, c, g, or t

<400>  88
gatcggaaga gcacacgtct gaactccagt cacnnnnnna tctcgtatgc cgtcttctgc       60

ttg                                                                     63


<210>  89
<211>  35
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  V3 consensus peptide

<400>  89

Cys Thr Arg Pro Asn Asn Asn Thr Arg Lys Ser Ile Arg Ile Gly Pro 
1               5                   10                  15      


Gly Gln Ala Phe Tyr Ala Thr Gly Asp Ile Ile Gly Asp Ile Arg Gln 
            20                  25                  30          


Ala His Cys 
        35  


<210>  90
<211>  35
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  V3 consensus peptide

<400>  90

Cys Thr Arg Pro Asn Asn Asn Thr Arg Lys Ser Ile His Ile Gly Pro 
1               5                   10                  15      


Gly Arg Ala Phe Tyr Thr Thr Gly Glu Ile Ile Gly Asp Ile Arg Gln 
            20                  25                  30          


Ala His Cys 
        35  


<210>  91
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  adapter sequence

<400>  91
aggaattcta cgctgagt                                                     18


<210>  92
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  adapter sequence

<400>  92
tgatagcaag cttgcc                                                       16


<210>  93
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7 forward primer

<400>  93
aatgatacgg caggaattct acgctgagt                                         29


<210>  94
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7 reverse primer

<400>  94
cgatcagcag aggcaagctt gctatca                                           27


<210>  95
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  R1 forward primer

<400>  95
tcgtcggcag cgtctccagt caggtgtgat gctc                                   34


<210>  96
<211>  49
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  R1 reverse primer

<400>  96
gtgggctcgg agatgtgtat aagagacagc aagacccgtt tagaggccc                   49


