
                                SEQUENCE LISTING

<110> Integrated Lipid Biofuels LLC
      Xiong, Xiaochao
      Chen, Shulin
      

<120> GENETICALLY ENGINEERED YEAST YARROWIA LIPOLYTICA 
      AND METHODS FOR PRODUCING BIO-BASED GLYCOLIC ACID 

<130> 480390.401WO

<140> PCT
<141> 2020-01-23

<150> US 62/795,927
<151> 2019-01-23

<160> 22

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 62
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic oligonucleotide

<400> 1
tctagaataa cttcgtataa tgtatgctat acgaagttat gactggccaa actgatctca      60
ag                                                                     62

<210> 2
<211> 56
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic oligonucleotide

<400> 2
ataacttcgt atagcataca ttatacgaag ttatatggtg tctgttttct acgtgt          56

<210> 3
<211> 40
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic oligonucleotide

<400> 3
agggcgaatt gggcccgacg tcagcacgtt cgatctagca                            40

<210> 4
<211> 40
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic oligonucleotide

<400> 4
ccatgcttag ttacaatgct tagccgatct aaaagtggag                            40

<210> 5
<211> 40
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic oligonucleotide

<400> 5
tcttcgtcat cccacctagc gattgcttac cattgtatgc                            40

<210> 6
<211> 40
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic oligonucleotide

<400> 6
ggagctctcc catatggtcg actccatgtc acagtttcgc                            40

<210> 7
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic oligonucleotide

<400> 7
caagggcatc aaactagctg                                                  20

<210> 8
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic oligonucleotide

<400> 8
gtttaacaca gccagatggg                                                  20

<210> 9
<211> 40
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic oligonucleotide

<400> 9
agggcgaatt gggcccgacg tcctattgtt cgattcggcg                            40

<210> 10
<211> 40
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic oligonucleotide

<400> 10
ccatgcttag ttacaatgct tatgtgcagg tacaacggaa                            40

<210> 11
<211> 40
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic oligonucleotide

<400> 11
gcatacaatg gtaagcaatc gcaagctcta agcgcgatgt                            40

<210> 12
<211> 40
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic oligonucleotide

<400> 12
ggagctctcc catatggtcg actgattctg tcgcccaact                            40

<210> 13
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic oligonucleotide

<400> 13
ccatatgatt ctgtgcctgc                                                  20

<210> 14
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic oligonucleotide

<400> 14
cgaggagtat ccttccacca                                                  20

<210> 15
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic oligonucleotide

<400> 15
tcctggaggc agaagaactt                                                  20

<210> 16
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic oligonucleotide

<400> 16
agcccttctg actcacgtat                                                  20

<210> 17
<211> 962
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic DNA

<400> 17
atggaggtcg gcttcctcgg actgggtatt atgggcaagg ctatgtcgat gaacctgctc      60
aagaacggct ttaaggtcac tgtgtggaac cgaaccctgt ctaagtgtga cgagctggtg     120
gagcacggcg cttctgtctg tgagtccccc gccgaggtca tcaagaagtg caagtacacc     180
attgccatgc tgtctgaccc ctgtgccgct ctgtccgtgg tcttcgacaa gggcggagtg     240
ctggagcaga tctgcgaggg caagggatac attgacatgt ctaccgtcga cgctgagact     300
tctctgaaga tcaacgaggc cattaccgga aagggtggcc gattcgtgga gggtcccgtc     360
tctggatcca agaagcccgc cgaggacggt cagctgatca ttctggccgc tggagacaag     420
gctctgttcg aggagtctat ccccgccttc gacgtgctgg gaaagcgatc cttctacctg     480
ggtcaagtgg gtaacggcgc caagatgaag ctgatcgtca acatgattat gggatctatg     540
atgaacgctt tctccgaggg tctggtcctg gccgacaagt ctggcctgtc ttccgacacc     600
ctgctggaca ttctggacct gggtgctatg accaacccca tgttcaaggg aaagggtccc     660
tccatgacca agtcttccta ccctcccgcc ttccccctga agcaccagca gaaggacatg     720
cgactggccc tggctctggg cgacgagaac gctgtgtcca tgcctgtggc cgctgccgct     780
aacgaggcct tcaagaaggc tcgatctctg ggcctgggcg acctggactt ctccgccgtg     840
atcgaggctg tcaagttcgc cgagtacatc gacggaattc tgcgaatggt caccggaggt     900
attacttcta ccgccgctat gggtgctggt gtgaccgagg atcagttcaa gtctaagctg     960
ta                                                                    962

<210> 18
<211> 18
<212> PRT
<213> Yarrowia lipolytica

<400> 18
Met Leu Ala Phe Lys Ser Leu Arg Pro Ser Ala Val Ser Arg Leu Ala
 1               5                  10                  15
Thr Ser


<210> 19
<211> 34
<212> PRT
<213> Yarrowia lipolytica

<400> 19
Met Leu Arg His Ala Leu Leu Lys Arg Thr Pro Val Leu Pro Arg Leu
 1               5                  10                  15
Thr Ser Arg Lys Ala Phe Val Pro Leu Ile Gln Lys Arg Lys Tyr Ser
            20                  25                  30
Asp Asp


<210> 20
<211> 34
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic oligonucleotide

<400> 20
ggcgcactgc agatgaaaac ccgtacacaa caaa                                  34

<210> 21
<211> 36
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic oligonucleotide

<400> 21
gcaattcccg ggttagaact gcgattcttc agtgga                                36

<210> 22
<211> 999
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic DNA

<400> 22
atgacccgaa cccccgtgaa cgtcaccgtc accggcgctg ctggtcaaat cggctacgcc      60
ttattattca gaatcgcctc cggtcagctg ctgggtgctg acgtccccgt caagctgcga     120
ctgctgggtt ctacccgatc tttaaaggct gctgagggca ccgccatgga gctggatgac     180
tgcgccttcc ctttattaca aggtatcgag atcactgatg accccaacgt ggccttcgac     240
ggcgctaacg tggctttatt agtgggcgct cgacctcgaa ccaagggtat ggagcgaggc     300
gacctcctcg aggccaacgg tggcatcttt aagccccaag gtaaggccat caacgaccac     360
gccgccgacg acatcaaggt tttagtcgtg ggcaaccccg ccaacaccaa cgctttaatc     420
gctcaagctg ccgctcccga cgtgcccgcc gaacgattca ctgccatgac ccgactcgac     480
cacaaccgag ctttaaccca gctcgccaag aagaccggtt ccactgtggc cgacattaag     540
cgactgacca tctggggcaa ccactccgcc acccagtacc ccgacatctt tcacgccacc     600
gtcgccggca aaaacgccgc cgagaccgtc aacgacgaga agtggctggc tgacgagttt     660
atccccactg tcgccaagcg aggcgccgct atcatcgagg ctcgaggcgc ttcttccgct     720
gcttctgccg ccaacgccgc catcgaccac gtctacactt gggtgaatgg caccgccgag     780
ggcgactgga cctctatggg tatcccctcc gacggctcct acggtgtgcc cgagggtatc     840
atctcctcct tccccgtcac caccaaggac ggttcctacg agattgtcca aggtctggac     900
atcaacgagt tctcccgagc ccgaatcgac gcctctgtga aggagctgtc cgaggagcga     960
gaagccgtcc gaggtctggg tctgatctct aagctgtaa                            999

