                         SEQUENCE LISTING

<110>  National University of Singapore
 
<120>  ISTHMIN 1 FOR TREATMENT OF LUNG INFLAMMATION

<130>  2017-096

<150>  SG 10201902000Y
<151>  2019-03-06

<160>  36    

<170>  PatentIn version 3.5

<210>  1
<211>  464
<212>  PRT
<213>  Homo sapiens

<400>  1

Met Val Arg Leu Ala Ala Glu Leu Leu Leu Leu Leu Gly Leu Leu Leu 
1               5                   10                  15      


Leu Thr Leu His Ile Thr Val Leu Arg Gly Ser Gly Ala Ala Asp Gly 
            20                  25                  30          


Pro Asp Ala Ala Ala Gly Asn Ala Ser Gln Ala Gln Leu Gln Asn Asn 
        35                  40                  45              


Leu Asn Val Gly Ser Asp Thr Thr Ser Glu Thr Ser Phe Ser Leu Ser 
    50                  55                  60                  


Lys Glu Ala Pro Arg Glu His Leu Asp His Gln Ala Ala His Gln Pro 
65                  70                  75                  80  


Phe Pro Arg Pro Arg Phe Arg Gln Glu Thr Gly His Pro Ser Leu Gln 
                85                  90                  95      


Arg Asp Phe Pro Arg Ser Phe Leu Leu Asp Leu Pro Asn Phe Pro Asp 
            100                 105                 110         


Leu Ser Lys Ala Asp Ile Asn Gly Gln Asn Pro Asn Ile Gln Val Thr 
        115                 120                 125             


Ile Glu Val Val Asp Gly Pro Asp Ser Glu Ala Asp Lys Asp Gln His 
    130                 135                 140                 


Pro Glu Asn Lys Pro Ser Trp Ser Val Pro Ser Pro Asp Trp Arg Ala 
145                 150                 155                 160 


Trp Trp Gln Arg Ser Leu Ser Leu Ala Arg Ala Asn Ser Gly Asp Gln 
                165                 170                 175     


Asp Tyr Lys Tyr Asp Ser Thr Ser Asp Asp Ser Asn Phe Leu Asn Pro 
            180                 185                 190         


Pro Arg Gly Trp Asp His Thr Ala Pro Gly His Arg Thr Phe Glu Thr 
        195                 200                 205             


Lys Asp Gln Pro Glu Tyr Asp Ser Thr Asp Gly Glu Gly Asp Trp Ser 
    210                 215                 220                 


Leu Trp Ser Val Cys Ser Val Thr Cys Gly Asn Gly Asn Gln Lys Arg 
225                 230                 235                 240 


Thr Arg Ser Cys Gly Tyr Ala Cys Thr Ala Thr Glu Ser Arg Thr Cys 
                245                 250                 255     


Asp Arg Pro Asn Cys Pro Gly Ile Glu Asp Thr Phe Arg Thr Ala Ala 
            260                 265                 270         


Thr Glu Val Ser Leu Leu Ala Gly Ser Glu Glu Phe Asn Ala Thr Lys 
        275                 280                 285             


Leu Phe Glu Val Asp Thr Asp Ser Cys Glu Arg Trp Met Ser Cys Lys 
    290                 295                 300                 


Ser Glu Phe Leu Lys Lys Tyr Met His Lys Val Met Asn Asp Leu Pro 
305                 310                 315                 320 


Ser Cys Pro Cys Ser Tyr Pro Thr Glu Val Ala Tyr Ser Thr Ala Asp 
                325                 330                 335     


Ile Phe Asp Arg Ile Lys Arg Lys Asp Phe Arg Trp Lys Asp Ala Ser 
            340                 345                 350         


Gly Pro Lys Glu Lys Leu Glu Ile Tyr Lys Pro Thr Ala Arg Tyr Cys 
        355                 360                 365             


Ile Arg Ser Met Leu Ser Leu Glu Ser Thr Thr Leu Ala Ala Gln His 
    370                 375                 380                 


Cys Cys Tyr Gly Asp Asn Met Gln Leu Ile Thr Arg Gly Lys Gly Ala 
385                 390                 395                 400 


Gly Thr Pro Asn Leu Ile Ser Thr Glu Phe Ser Ala Glu Leu His Tyr 
                405                 410                 415     


Lys Val Asp Val Leu Pro Trp Ile Ile Cys Lys Gly Asp Trp Ser Arg 
            420                 425                 430         


Tyr Asn Glu Ala Arg Pro Pro Asn Asn Gly Gln Lys Cys Thr Glu Ser 
        435                 440                 445             


Pro Ser Asp Glu Asp Tyr Ile Lys Gln Phe Gln Glu Ala Arg Glu Tyr 
    450                 455                 460                 


<210>  2
<211>  461
<212>  PRT
<213>  Mus musculus

<400>  2

Met Val Arg Leu Ala Ala Glu Leu Leu Leu Leu Leu Gly Leu Leu Leu 
1               5                   10                  15      


Leu Thr Leu His Ile Thr Val Leu Arg Gly Ser Gly Ala Ser Asp Arg 
            20                  25                  30          


Gln Asp Ala Ala Ala Gly Asn Val Ser Gly Ser Gln Leu Gln Asn Asn 
        35                  40                  45              


Leu Asn Leu Glu Ser Asp Ser Thr Ser Glu Thr Ser Phe Pro Leu Ser 
    50                  55                  60                  


Lys Glu Ala Pro Glu Glu His Gln Val Val His Gln Pro Phe Pro Arg 
65                  70                  75                  80  


Gln Arg Phe Pro Pro Glu Thr Gly His Pro Ser Leu Gln Arg Asp Gly 
                85                  90                  95      


Pro Arg Ser Phe Leu Leu Asp Leu Pro Asn Phe Pro Asp Leu Ser Lys 
            100                 105                 110         


Ala Asp Ile Asn Gly Gln Asn Pro Asn Ile Gln Val Thr Ile Glu Val 
        115                 120                 125             


Val Asp Gly Pro Asp Ser Glu Ala Glu Lys Asp Gln His Pro Glu Asn 
    130                 135                 140                 


Lys Pro Ser Trp Ser Leu Pro Ala Pro Asp Trp Arg Ala Trp Trp Gln 
145                 150                 155                 160 


Arg Ser Leu Ser Leu Ala Arg Thr Asn Ser Gly Asp Gln Asp Asp Lys 
                165                 170                 175     


Tyr Asp Ser Thr Ser Asp Asp Ser Asn Phe Leu Ser Val Pro Arg Gly 
            180                 185                 190         


Trp Asp Arg Pro Ala Pro Gly His Arg Thr Phe Glu Thr Lys Glu Gln 
        195                 200                 205             


Pro Glu Tyr Asp Ser Thr Asp Gly Glu Gly Asp Trp Ser Leu Trp Ser 
    210                 215                 220                 


Val Cys Ser Val Thr Cys Gly Asn Gly Asn Gln Lys Arg Thr Arg Ser 
225                 230                 235                 240 


Cys Gly Tyr Ala Cys Ile Ala Thr Glu Ser Arg Thr Cys Asp Arg Pro 
                245                 250                 255     


Asn Cys Pro Gly Ile Glu Asp Thr Phe Arg Thr Ala Ala Thr Glu Val 
            260                 265                 270         


Ser Leu Leu Ala Gly Ser Glu Glu Phe Asn Ala Thr Lys Leu Phe Glu 
        275                 280                 285             


Val Asp Met Asp Ser Cys Glu Arg Trp Met Ser Cys Lys Ser Glu Phe 
    290                 295                 300                 


Leu Lys Lys Tyr Met His Lys Val Ile Asn Asp Leu Pro Ser Cys Pro 
305                 310                 315                 320 


Cys Ser Tyr Pro Thr Glu Val Ala Tyr Ser Thr Ala Asp Ile Phe Asp 
                325                 330                 335     


Arg Ile Lys Arg Lys Asp Phe Arg Trp Lys Asp Ala Ser Gly Pro Lys 
            340                 345                 350         


Glu Lys Leu Glu Ile Tyr Lys Pro Thr Ala Arg Tyr Cys Ile Arg Ser 
        355                 360                 365             


Met Leu Ser Leu Glu Ser Thr Thr Leu Ala Ala Gln His Cys Cys Tyr 
    370                 375                 380                 


Gly Asp Asn Met Gln Leu Ile Thr Arg Gly Lys Gly Ala Gly Thr Pro 
385                 390                 395                 400 


Asn Leu Ile Ser Thr Glu Phe Ser Ala Glu Leu His Tyr Lys Val Asp 
                405                 410                 415     


Val Leu Pro Trp Ile Ile Cys Lys Gly Asp Trp Ser Arg Tyr Asn Glu 
            420                 425                 430         


Ala Arg Pro Pro Asn Asn Gly Gln Lys Cys Thr Glu Ser Pro Ser Asp 
        435                 440                 445             


Glu Asp Tyr Ile Lys Gln Phe Gln Glu Ala Arg Glu Tyr 
    450                 455                 460     


<210>  3
<211>  439
<212>  PRT
<213>  Homo sapiens

<400>  3

Gly Ser Gly Ala Ala Asp Gly Pro Asp Ala Ala Ala Gly Asn Ala Ser 
1               5                   10                  15      


Gln Ala Gln Leu Gln Asn Asn Leu Asn Val Gly Ser Asp Thr Thr Ser 
            20                  25                  30          


Glu Thr Ser Phe Ser Leu Ser Lys Glu Ala Pro Arg Glu His Leu Asp 
        35                  40                  45              


His Gln Ala Ala His Gln Pro Phe Pro Arg Pro Arg Phe Arg Gln Glu 
    50                  55                  60                  


Thr Gly His Pro Ser Leu Gln Arg Asp Phe Pro Arg Ser Phe Leu Leu 
65                  70                  75                  80  


Asp Leu Pro Asn Phe Pro Asp Leu Ser Lys Ala Asp Ile Asn Gly Gln 
                85                  90                  95      


Asn Pro Asn Ile Gln Val Thr Ile Glu Val Val Asp Gly Pro Asp Ser 
            100                 105                 110         


Glu Ala Asp Lys Asp Gln His Pro Glu Asn Lys Pro Ser Trp Ser Val 
        115                 120                 125             


Pro Ser Pro Asp Trp Arg Ala Trp Trp Gln Arg Ser Leu Ser Leu Ala 
    130                 135                 140                 


Arg Ala Asn Ser Gly Asp Gln Asp Tyr Lys Tyr Asp Ser Thr Ser Asp 
145                 150                 155                 160 


Asp Ser Asn Phe Leu Asn Pro Pro Arg Gly Trp Asp His Thr Ala Pro 
                165                 170                 175     


Gly His Arg Thr Phe Glu Thr Lys Asp Gln Pro Glu Tyr Asp Ser Thr 
            180                 185                 190         


Asp Gly Glu Gly Asp Trp Ser Leu Trp Ser Val Cys Ser Val Thr Cys 
        195                 200                 205             


Gly Asn Gly Asn Gln Lys Arg Thr Arg Ser Cys Gly Tyr Ala Cys Thr 
    210                 215                 220                 


Ala Thr Glu Ser Arg Thr Cys Asp Arg Pro Asn Cys Pro Gly Ile Glu 
225                 230                 235                 240 


Asp Thr Phe Arg Thr Ala Ala Thr Glu Val Ser Leu Leu Ala Gly Ser 
                245                 250                 255     


Glu Glu Phe Asn Ala Thr Lys Leu Phe Glu Val Asp Thr Asp Ser Cys 
            260                 265                 270         


Glu Arg Trp Met Ser Cys Lys Ser Glu Phe Leu Lys Lys Tyr Met His 
        275                 280                 285             


Lys Val Met Asn Asp Leu Pro Ser Cys Pro Cys Ser Tyr Pro Thr Glu 
    290                 295                 300                 


Val Ala Tyr Ser Thr Ala Asp Ile Phe Asp Arg Ile Lys Arg Lys Asp 
305                 310                 315                 320 


Phe Arg Trp Lys Asp Ala Ser Gly Pro Lys Glu Lys Leu Glu Ile Tyr 
                325                 330                 335     


Lys Pro Thr Ala Arg Tyr Cys Ile Arg Ser Met Leu Ser Leu Glu Ser 
            340                 345                 350         


Thr Thr Leu Ala Ala Gln His Cys Cys Tyr Gly Asp Asn Met Gln Leu 
        355                 360                 365             


Ile Thr Arg Gly Lys Gly Ala Gly Thr Pro Asn Leu Ile Ser Thr Glu 
    370                 375                 380                 


Phe Ser Ala Glu Leu His Tyr Lys Val Asp Val Leu Pro Trp Ile Ile 
385                 390                 395                 400 


Cys Lys Gly Asp Trp Ser Arg Tyr Asn Glu Ala Arg Pro Pro Asn Asn 
                405                 410                 415     


Gly Gln Lys Cys Thr Glu Ser Pro Ser Asp Glu Asp Tyr Ile Lys Gln 
            420                 425                 430         


Phe Gln Glu Ala Arg Glu Tyr 
        435                 


<210>  4
<211>  436
<212>  PRT
<213>  Mus musculus

<400>  4

Gly Ser Gly Ala Ser Asp Arg Gln Asp Ala Ala Ala Gly Asn Val Ser 
1               5                   10                  15      


Gly Ser Gln Leu Gln Asn Asn Leu Asn Leu Glu Ser Asp Ser Thr Ser 
            20                  25                  30          


Glu Thr Ser Phe Pro Leu Ser Lys Glu Ala Pro Glu Glu His Gln Val 
        35                  40                  45              


Val His Gln Pro Phe Pro Arg Gln Arg Phe Pro Pro Glu Thr Gly His 
    50                  55                  60                  


Pro Ser Leu Gln Arg Asp Gly Pro Arg Ser Phe Leu Leu Asp Leu Pro 
65                  70                  75                  80  


Asn Phe Pro Asp Leu Ser Lys Ala Asp Ile Asn Gly Gln Asn Pro Asn 
                85                  90                  95      


Ile Gln Val Thr Ile Glu Val Val Asp Gly Pro Asp Ser Glu Ala Glu 
            100                 105                 110         


Lys Asp Gln His Pro Glu Asn Lys Pro Ser Trp Ser Leu Pro Ala Pro 
        115                 120                 125             


Asp Trp Arg Ala Trp Trp Gln Arg Ser Leu Ser Leu Ala Arg Thr Asn 
    130                 135                 140                 


Ser Gly Asp Gln Asp Asp Lys Tyr Asp Ser Thr Ser Asp Asp Ser Asn 
145                 150                 155                 160 


Phe Leu Ser Val Pro Arg Gly Trp Asp Arg Pro Ala Pro Gly His Arg 
                165                 170                 175     


Thr Phe Glu Thr Lys Glu Gln Pro Glu Tyr Asp Ser Thr Asp Gly Glu 
            180                 185                 190         


Gly Asp Trp Ser Leu Trp Ser Val Cys Ser Val Thr Cys Gly Asn Gly 
        195                 200                 205             


Asn Gln Lys Arg Thr Arg Ser Cys Gly Tyr Ala Cys Ile Ala Thr Glu 
    210                 215                 220                 


Ser Arg Thr Cys Asp Arg Pro Asn Cys Pro Gly Ile Glu Asp Thr Phe 
225                 230                 235                 240 


Arg Thr Ala Ala Thr Glu Val Ser Leu Leu Ala Gly Ser Glu Glu Phe 
                245                 250                 255     


Asn Ala Thr Lys Leu Phe Glu Val Asp Met Asp Ser Cys Glu Arg Trp 
            260                 265                 270         


Met Ser Cys Lys Ser Glu Phe Leu Lys Lys Tyr Met His Lys Val Ile 
        275                 280                 285             


Asn Asp Leu Pro Ser Cys Pro Cys Ser Tyr Pro Thr Glu Val Ala Tyr 
    290                 295                 300                 


Ser Thr Ala Asp Ile Phe Asp Arg Ile Lys Arg Lys Asp Phe Arg Trp 
305                 310                 315                 320 


Lys Asp Ala Ser Gly Pro Lys Glu Lys Leu Glu Ile Tyr Lys Pro Thr 
                325                 330                 335     


Ala Arg Tyr Cys Ile Arg Ser Met Leu Ser Leu Glu Ser Thr Thr Leu 
            340                 345                 350         


Ala Ala Gln His Cys Cys Tyr Gly Asp Asn Met Gln Leu Ile Thr Arg 
        355                 360                 365             


Gly Lys Gly Ala Gly Thr Pro Asn Leu Ile Ser Thr Glu Phe Ser Ala 
    370                 375                 380                 


Glu Leu His Tyr Lys Val Asp Val Leu Pro Trp Ile Ile Cys Lys Gly 
385                 390                 395                 400 


Asp Trp Ser Arg Tyr Asn Glu Ala Arg Pro Pro Asn Asn Gly Gln Lys 
                405                 410                 415     


Cys Thr Glu Ser Pro Ser Asp Glu Asp Tyr Ile Lys Gln Phe Gln Glu 
            420                 425                 430         


Ala Arg Glu Tyr 
        435     


<210>  5
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA1

<400>  5
cugcacauca cgguucugcg cgg                                               23


<210>  6
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA2

<400>  6
gcggauccgg agccuccgac cgg                                               23


<210>  7
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer P1

<400>  7
cagctcctgg gattgctccg                                                   20


<210>  8
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer P2

<400>  8
ccttctgcaa tgtaccaagc tct                                               23


<210>  9
<211>  458
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mouse rISM1 with His Tag

<400>  9

Met His His His His His His Ser Ser Gly Leu Val Pro Arg Gly Ser 
1               5                   10                  15      


Gly Ala Ser Asp Arg Gln Asp Ala Ala Ala Gly Asn Val Ser Gly Ser 
            20                  25                  30          


Gln Leu Gln Asn Asn Leu Asn Leu Glu Ser Asp Ser Thr Ser Glu Thr 
        35                  40                  45              


Ser Phe Pro Leu Ser Lys Glu Ala Pro Glu Glu His Gln Val Val His 
    50                  55                  60                  


Gln Pro Phe Pro Arg Gln Arg Phe Pro Pro Glu Thr Gly His Pro Ser 
65                  70                  75                  80  


Leu Gln Arg Asp Gly Pro Arg Ser Phe Leu Leu Asp Leu Pro Asn Phe 
                85                  90                  95      


Pro Asp Leu Ser Lys Ala Asp Ile Asn Gly Gln Asn Pro Asn Ile Gln 
            100                 105                 110         


Val Thr Ile Glu Val Val Asp Gly Pro Asp Ser Glu Ala Glu Lys Asp 
        115                 120                 125             


Gln His Pro Glu Asn Lys Pro Ser Trp Ser Leu Pro Ala Pro Asp Trp 
    130                 135                 140                 


Arg Ala Trp Trp Gln Arg Ser Leu Ser Leu Ala Arg Thr Asn Ser Gly 
145                 150                 155                 160 


Asp Gln Asp Asp Lys Tyr Asp Ser Thr Ser Asp Asp Ser Asn Phe Leu 
                165                 170                 175     


Ser Val Pro Arg Gly Trp Asp Arg Pro Ala Pro Gly His Arg Thr Phe 
            180                 185                 190         


Glu Thr Lys Glu Gln Pro Glu Tyr Asp Ser Thr Asp Gly Glu Gly Asp 
        195                 200                 205             


Trp Ser Leu Trp Ser Val Cys Ser Val Thr Cys Gly Asn Gly Asn Gln 
    210                 215                 220                 


Lys Arg Thr Arg Ser Cys Gly Tyr Ala Cys Ile Ala Thr Glu Ser Arg 
225                 230                 235                 240 


Thr Cys Asp Arg Pro Asn Cys Pro Gly Ile Glu Asp Thr Phe Arg Thr 
                245                 250                 255     


Ala Ala Thr Glu Val Ser Leu Leu Ala Gly Ser Glu Glu Phe Asn Ala 
            260                 265                 270         


Thr Lys Leu Phe Glu Val Asp Met Asp Ser Cys Glu Arg Trp Met Ser 
        275                 280                 285             


Cys Lys Ser Glu Phe Leu Lys Lys Tyr Met His Lys Val Ile Asn Asp 
    290                 295                 300                 


Leu Pro Ser Cys Pro Cys Ser Tyr Pro Thr Glu Val Ala Tyr Ser Thr 
305                 310                 315                 320 


Ala Asp Ile Phe Asp Arg Ile Lys Arg Lys Asp Phe Arg Trp Lys Asp 
                325                 330                 335     


Ala Ser Gly Pro Lys Glu Lys Leu Glu Ile Tyr Lys Pro Thr Ala Arg 
            340                 345                 350         


Tyr Cys Ile Arg Ser Met Leu Ser Leu Glu Ser Thr Thr Leu Ala Ala 
        355                 360                 365             


Gln His Cys Cys Tyr Gly Asp Asn Met Gln Leu Ile Thr Arg Gly Lys 
    370                 375                 380                 


Gly Ala Gly Thr Pro Asn Leu Ile Ser Thr Glu Phe Ser Ala Glu Leu 
385                 390                 395                 400 


His Tyr Lys Val Asp Val Leu Pro Trp Ile Ile Cys Lys Gly Asp Trp 
                405                 410                 415     


Ser Arg Tyr Asn Glu Ala Arg Pro Pro Asn Asn Gly Gln Lys Cys Thr 
            420                 425                 430         


Glu Ser Pro Ser Asp Glu Asp Tyr Ile Lys Gln Phe Gln Glu Ala Arg 
        435                 440                 445             


Glu Tyr Leu Glu His His His His His His 
    450                 455             


<210>  10
<211>  164
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human ISM1 AMOP domain

<400>  10

Phe Glu Val Asp Thr Asp Ser Cys Glu Arg Trp Met Ser Cys Lys Ser 
1               5                   10                  15      


Glu Phe Leu Lys Lys Tyr Met His Lys Val Met Asn Asp Leu Pro Ser 
            20                  25                  30          


Cys Pro Cys Ser Tyr Pro Thr Glu Val Ala Tyr Ser Thr Ala Asp Ile 
        35                  40                  45              


Phe Asp Arg Ile Lys Arg Lys Asp Phe Arg Trp Lys Asp Ala Ser Gly 
    50                  55                  60                  


Pro Lys Glu Lys Leu Glu Ile Tyr Lys Pro Thr Ala Arg Tyr Cys Ile 
65                  70                  75                  80  


Arg Ser Met Leu Ser Leu Glu Ser Thr Thr Leu Ala Ala Gln His Cys 
                85                  90                  95      


Cys Tyr Gly Asp Asn Met Gln Leu Ile Thr Arg Gly Lys Gly Ala Gly 
            100                 105                 110         


Thr Pro Asn Leu Ile Ser Thr Glu Phe Ser Ala Glu Leu His Tyr Lys 
        115                 120                 125             


Val Asp Val Leu Pro Trp Ile Ile Cys Lys Gly Asp Trp Ser Arg Tyr 
    130                 135                 140                 


Asn Glu Ala Arg Pro Pro Asn Asn Gly Gln Lys Cys Thr Glu Ser Pro 
145                 150                 155                 160 


Ser Asp Glu Asp 
                


<210>  11
<211>  164
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mouse ISM1 AMOP domain

<400>  11

Phe Glu Val Asp Met Asp Ser Cys Glu Arg Trp Met Ser Cys Lys Ser 
1               5                   10                  15      


Glu Phe Leu Lys Lys Tyr Met His Lys Val Ile Asn Asp Leu Pro Ser 
            20                  25                  30          


Cys Pro Cys Ser Tyr Pro Thr Glu Val Ala Tyr Ser Thr Ala Asp Ile 
        35                  40                  45              


Phe Asp Arg Ile Lys Arg Lys Asp Phe Arg Trp Lys Asp Ala Ser Gly 
    50                  55                  60                  


Pro Lys Glu Lys Leu Glu Ile Tyr Lys Pro Thr Ala Arg Tyr Cys Ile 
65                  70                  75                  80  


Arg Ser Met Leu Ser Leu Glu Ser Thr Thr Leu Ala Ala Gln His Cys 
                85                  90                  95      


Cys Tyr Gly Asp Asn Met Gln Leu Ile Thr Arg Gly Lys Gly Ala Gly 
            100                 105                 110         


Thr Pro Asn Leu Ile Ser Thr Glu Phe Ser Ala Glu Leu His Tyr Lys 
        115                 120                 125             


Val Asp Val Leu Pro Trp Ile Ile Cys Lys Gly Asp Trp Ser Arg Tyr 
    130                 135                 140                 


Asn Glu Ala Arg Pro Pro Asn Asn Gly Gln Lys Cys Thr Glu Ser Pro 
145                 150                 155                 160 


Ser Asp Glu Asp 
                


<210>  12
<211>  103
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  N-terminal portion of C-terminal region of mouse ISM1

<400>  12

Glu Val Ser Leu Leu Ala Gly Ser Glu Glu Phe Asn Ala Thr Lys Leu 
1               5                   10                  15      


Phe Glu Val Asp Met Asp Ser Cys Glu Arg Trp Met Ser Cys Lys Ser 
            20                  25                  30          


Glu Phe Leu Lys Lys Tyr Met His Lys Val Ile Asn Asp Leu Pro Ser 
        35                  40                  45              


Cys Pro Cys Ser Tyr Pro Thr Glu Val Ala Tyr Ser Thr Ala Asp Ile 
    50                  55                  60                  


Phe Asp Arg Ile Lys Arg Lys Asp Phe Arg Trp Lys Asp Ala Ser Gly 
65                  70                  75                  80  


Pro Lys Glu Lys Leu Glu Ile Tyr Lys Pro Thr Ala Arg Tyr Cys Ile 
                85                  90                  95      


Arg Ser Met Leu Ser Leu Glu 
            100             


<210>  13
<211>  191
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  C-terminal region of mouse ISM1 containing AMOP but not TSR

<400>  13

Glu Val Ser Leu Leu Ala Gly Ser Glu Glu Phe Asn Ala Thr Lys Leu 
1               5                   10                  15      


Phe Glu Val Asp Met Asp Ser Cys Glu Arg Trp Met Ser Cys Lys Ser 
            20                  25                  30          


Glu Phe Leu Lys Lys Tyr Met His Lys Val Ile Asn Asp Leu Pro Ser 
        35                  40                  45              


Cys Pro Cys Ser Tyr Pro Thr Glu Val Ala Tyr Ser Thr Ala Asp Ile 
    50                  55                  60                  


Phe Asp Arg Ile Lys Arg Lys Asp Phe Arg Trp Lys Asp Ala Ser Gly 
65                  70                  75                  80  


Pro Lys Glu Lys Leu Glu Ile Tyr Lys Pro Thr Ala Arg Tyr Cys Ile 
                85                  90                  95      


Arg Ser Met Leu Ser Leu Glu Ser Thr Thr Leu Ala Ala Gln His Cys 
            100                 105                 110         


Cys Tyr Gly Asp Asn Met Gln Leu Ile Thr Arg Gly Lys Gly Ala Gly 
        115                 120                 125             


Thr Pro Asn Leu Ile Ser Thr Glu Phe Ser Ala Glu Leu His Tyr Lys 
    130                 135                 140                 


Val Asp Val Leu Pro Trp Ile Ile Cys Lys Gly Asp Trp Ser Arg Tyr 
145                 150                 155                 160 


Asn Glu Ala Arg Pro Pro Asn Asn Gly Gln Lys Cys Thr Glu Ser Pro 
                165                 170                 175     


Ser Asp Glu Asp Tyr Ile Lys Gln Phe Gln Glu Ala Arg Glu Tyr 
            180                 185                 190     


<210>  14
<211>  87
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ISM1C-N with KD341AA mutation

<400>  14

Phe Glu Val Asp Met Asp Ser Cys Glu Arg Trp Met Ser Cys Lys Ser 
1               5                   10                  15      


Glu Phe Leu Lys Lys Tyr Met His Lys Val Ile Asn Asp Leu Pro Ser 
            20                  25                  30          


Cys Pro Cys Ser Tyr Pro Thr Glu Val Ala Tyr Ser Thr Ala Asp Ile 
        35                  40                  45              


Phe Asp Arg Ile Lys Arg Ala Ala Phe Arg Trp Lys Asp Ala Ser Gly 
    50                  55                  60                  


Pro Lys Glu Lys Leu Glu Ile Tyr Lys Pro Thr Ala Arg Tyr Cys Ile 
65                  70                  75                  80  


Arg Ser Met Leu Ser Leu Glu 
                85          


<210>  15
<211>  87
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ISM1C-N with RKD340AAA mutation

<400>  15

Phe Glu Val Asp Met Asp Ser Cys Glu Arg Trp Met Ser Cys Lys Ser 
1               5                   10                  15      


Glu Phe Leu Lys Lys Tyr Met His Lys Val Ile Asn Asp Leu Pro Ser 
            20                  25                  30          


Cys Pro Cys Ser Tyr Pro Thr Glu Val Ala Tyr Ser Thr Ala Asp Ile 
        35                  40                  45              


Phe Asp Arg Ile Lys Ala Ala Ala Phe Arg Trp Lys Asp Ala Ser Gly 
    50                  55                  60                  


Pro Lys Glu Lys Leu Glu Ile Tyr Lys Pro Thr Ala Arg Tyr Cys Ile 
65                  70                  75                  80  


Arg Ser Met Leu Ser Leu Glu 
                85          


<210>  16
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7E1 forward PCR primer

<400>  16
cagctcctgg gattgctccg                                                   20


<210>  17
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7E1 reverse PCR primer

<400>  17
taagacttct tcctggtgcc aaa                                               23


<210>  18
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward PCR primer for mouse genotyping

<400>  18
gacagctcct gggattgctc c                                                 21


<210>  19
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  reverse PCR primer for mouse genotyping

<400>  19
ttctgcaatg taccaagctc tct                                               23


<210>  20
<211>  103
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HUMAN ISM1C-N

<400>  20

Glu Val Ser Leu Leu Ala Gly Ser Glu Glu Phe Asn Ala Thr Lys Leu 
1               5                   10                  15      


Phe Glu Val Asp Thr Asp Ser Cys Glu Arg Trp Met Ser Cys Lys Ser 
            20                  25                  30          


Glu Phe Leu Lys Lys Tyr Met His Lys Val Met Asn Asp Leu Pro Ser 
        35                  40                  45              


Cys Pro Cys Ser Tyr Pro Thr Glu Val Ala Tyr Ser Thr Ala Asp Ile 
    50                  55                  60                  


Phe Asp Arg Ile Lys Arg Lys Asp Phe Arg Trp Lys Asp Ala Ser Gly 
65                  70                  75                  80  


Pro Lys Glu Lys Leu Glu Ile Tyr Lys Pro Thr Ala Arg Tyr Cys Ile 
                85                  90                  95      


Arg Ser Met Leu Ser Leu Glu 
            100             


<210>  21
<211>  191
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  human ISM1C

<400>  21

Glu Val Ser Leu Leu Ala Gly Ser Glu Glu Phe Asn Ala Thr Lys Leu 
1               5                   10                  15      


Phe Glu Val Asp Thr Asp Ser Cys Glu Arg Trp Met Ser Cys Lys Ser 
            20                  25                  30          


Glu Phe Leu Lys Lys Tyr Met His Lys Val Met Asn Asp Leu Pro Ser 
        35                  40                  45              


Cys Pro Cys Ser Tyr Pro Thr Glu Val Ala Tyr Ser Thr Ala Asp Ile 
    50                  55                  60                  


Phe Asp Arg Ile Lys Arg Lys Asp Phe Arg Trp Lys Asp Ala Ser Gly 
65                  70                  75                  80  


Pro Lys Glu Lys Leu Glu Ile Tyr Lys Pro Thr Ala Arg Tyr Cys Ile 
                85                  90                  95      


Arg Ser Met Leu Ser Leu Glu Ser Thr Thr Leu Ala Ala Gln His Cys 
            100                 105                 110         


Cys Tyr Gly Asp Asn Met Gln Leu Ile Thr Arg Gly Lys Gly Ala Gly 
        115                 120                 125             


Thr Pro Asn Leu Ile Ser Thr Glu Phe Ser Ala Glu Leu His Tyr Lys 
    130                 135                 140                 


Val Asp Val Leu Pro Trp Ile Ile Cys Lys Gly Asp Trp Ser Arg Tyr 
145                 150                 155                 160 


Asn Glu Ala Arg Pro Pro Asn Asn Gly Gln Lys Cys Thr Glu Ser Pro 
                165                 170                 175     


Ser Asp Glu Asp Tyr Ile Lys Gln Phe Gln Glu Ala Arg Glu Tyr 
            180                 185                 190     


<210>  22
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  genotyping primer 1

<400>  22
cgcgcgactc aagaggatgg                                                   20


<210>  23
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  genotyping primer 2

<400>  23
actgggaccc gctgacgttg                                                   20


<210>  24
<211>  175
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  C-terminal AMOP domain aa 287-461 mouse

<400>  24

Phe Glu Val Asp Met Asp Ser Cys Glu Arg Trp Met Ser Cys Lys Ser 
1               5                   10                  15      


Glu Phe Leu Lys Lys Tyr Met His Lys Val Ile Asn Asp Leu Pro Ser 
            20                  25                  30          


Cys Pro Cys Ser Tyr Pro Thr Glu Val Ala Tyr Ser Thr Ala Asp Ile 
        35                  40                  45              


Phe Asp Arg Ile Lys Arg Lys Asp Phe Arg Trp Lys Asp Ala Ser Gly 
    50                  55                  60                  


Pro Lys Glu Lys Leu Glu Ile Tyr Lys Pro Thr Ala Arg Tyr Cys Ile 
65                  70                  75                  80  


Arg Ser Met Leu Ser Leu Glu Ser Thr Thr Leu Ala Ala Gln His Cys 
                85                  90                  95      


Cys Tyr Gly Asp Asn Met Gln Leu Ile Thr Arg Gly Lys Gly Ala Gly 
            100                 105                 110         


Thr Pro Asn Leu Ile Ser Thr Glu Phe Ser Ala Glu Leu His Tyr Lys 
        115                 120                 125             


Val Asp Val Leu Pro Trp Ile Ile Cys Lys Gly Asp Trp Ser Arg Tyr 
    130                 135                 140                 


Asn Glu Ala Arg Pro Pro Asn Asn Gly Gln Lys Cys Thr Glu Ser Pro 
145                 150                 155                 160 


Ser Asp Glu Asp Tyr Ile Lys Gln Phe Gln Glu Ala Arg Glu Tyr 
                165                 170                 175 


<210>  25
<211>  175
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  C-terminal AMOP domain aa 287-461 human

<400>  25

Phe Glu Val Asp Thr Asp Ser Cys Glu Arg Trp Met Ser Cys Lys Ser 
1               5                   10                  15      


Glu Phe Leu Lys Lys Tyr Met His Lys Val Met Asn Asp Leu Pro Ser 
            20                  25                  30          


Cys Pro Cys Ser Tyr Pro Thr Glu Val Ala Tyr Ser Thr Ala Asp Ile 
        35                  40                  45              


Phe Asp Arg Ile Lys Arg Lys Asp Phe Arg Trp Lys Asp Ala Ser Gly 
    50                  55                  60                  


Pro Lys Glu Lys Leu Glu Ile Tyr Lys Pro Thr Ala Arg Tyr Cys Ile 
65                  70                  75                  80  


Arg Ser Met Leu Ser Leu Glu Ser Thr Thr Leu Ala Ala Gln His Cys 
                85                  90                  95      


Cys Tyr Gly Asp Asn Met Gln Leu Ile Thr Arg Gly Lys Gly Ala Gly 
            100                 105                 110         


Thr Pro Asn Leu Ile Ser Thr Glu Phe Ser Ala Glu Leu His Tyr Lys 
        115                 120                 125             


Val Asp Val Leu Pro Trp Ile Ile Cys Lys Gly Asp Trp Ser Arg Tyr 
    130                 135                 140                 


Asn Glu Ala Arg Pro Pro Asn Asn Gly Gln Lys Cys Thr Glu Ser Pro 
145                 150                 155                 160 


Ser Asp Glu Asp Tyr Ile Lys Gln Phe Gln Glu Ala Arg Glu Tyr 
                165                 170                 175 


<210>  26
<211>  87
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  N-terminal subportion of C-terminal region of mouse ISM1

<400>  26

Phe Glu Val Asp Met Asp Ser Cys Glu Arg Trp Met Ser Cys Lys Ser 
1               5                   10                  15      


Glu Phe Leu Lys Lys Tyr Met His Lys Val Ile Asn Asp Leu Pro Ser 
            20                  25                  30          


Cys Pro Cys Ser Tyr Pro Thr Glu Val Ala Tyr Ser Thr Ala Asp Ile 
        35                  40                  45              


Phe Asp Arg Ile Lys Arg Lys Asp Phe Arg Trp Lys Asp Ala Ser Gly 
    50                  55                  60                  


Pro Lys Glu Lys Leu Glu Ile Tyr Lys Pro Thr Ala Arg Tyr Cys Ile 
65                  70                  75                  80  


Arg Ser Met Leu Ser Leu Glu 
                85          


<210>  27
<211>  87
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  human ISM1C-N subportion

<400>  27

Phe Glu Val Asp Thr Asp Ser Cys Glu Arg Trp Met Ser Cys Lys Ser 
1               5                   10                  15      


Glu Phe Leu Lys Lys Tyr Met His Lys Val Met Asn Asp Leu Pro Ser 
            20                  25                  30          


Cys Pro Cys Ser Tyr Pro Thr Glu Val Ala Tyr Ser Thr Ala Asp Ile 
        35                  40                  45              


Phe Asp Arg Ile Lys Arg Lys Asp Phe Arg Trp Lys Asp Ala Ser Gly 
    50                  55                  60                  


Pro Lys Glu Lys Leu Glu Ile Tyr Lys Pro Thr Ala Arg Tyr Cys Ile 
65                  70                  75                  80  


Arg Ser Met Leu Ser Leu Glu 
                85          


<210>  28
<211>  548
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  bacISM126-461

<400>  28

Met Gly Ser Ser His His His His His His Ser Gln Gly Ser Met Ser 
1               5                   10                  15      


Asp Ser Glu Val Asn Gln Glu Ala Lys Pro Glu Val Lys Pro Glu Val 
            20                  25                  30          


Lys Pro Glu Thr His Ile Asn Leu Lys Val Ser Asp Gly Ser Ser Glu 
        35                  40                  45              


Ile Phe Phe Lys Ile Lys Lys Thr Thr Pro Leu Arg Arg Leu Met Glu 
    50                  55                  60                  


Ala Phe Ala Lys Arg Gln Gly Lys Glu Met Asp Ser Leu Arg Phe Leu 
65                  70                  75                  80  


Tyr Asp Gly Ile Arg Ile Gln Ala Asp Gln Thr Pro Glu Asp Leu Asp 
                85                  90                  95      


Met Glu Asp Asn Asp Ile Ile Glu Ala His Arg Glu Gln Ile Gly Gly 
            100                 105                 110         


Gly Ser Gly Ala Ser Asp Arg Gln Asp Ala Ala Ala Gly Asn Val Ser 
        115                 120                 125             


Gly Ser Gln Leu Gln Asn Asn Leu Asn Leu Glu Ser Asp Ser Thr Ser 
    130                 135                 140                 


Glu Thr Ser Phe Pro Leu Ser Lys Glu Ala Pro Glu Glu His Gln Val 
145                 150                 155                 160 


Val His Gln Pro Phe Pro Arg Gln Arg Phe Pro Pro Glu Thr Gly His 
                165                 170                 175     


Pro Ser Leu Gln Arg Asp Gly Pro Arg Ser Phe Leu Leu Asp Leu Pro 
            180                 185                 190         


Asn Phe Pro Asp Leu Ser Lys Ala Asp Ile Asn Gly Gln Asn Pro Asn 
        195                 200                 205             


Ile Gln Val Thr Ile Glu Val Val Asp Gly Pro Asp Ser Glu Ala Glu 
    210                 215                 220                 


Lys Asp Gln His Pro Glu Asn Lys Pro Ser Trp Ser Leu Pro Ala Pro 
225                 230                 235                 240 


Asp Trp Arg Ala Trp Trp Gln Arg Ser Leu Ser Leu Ala Arg Thr Asn 
                245                 250                 255     


Ser Gly Asp Gln Asp Asp Lys Tyr Asp Ser Thr Ser Asp Asp Ser Asn 
            260                 265                 270         


Phe Leu Ser Val Pro Arg Gly Trp Asp Arg Pro Ala Pro Gly His Arg 
        275                 280                 285             


Thr Phe Glu Thr Lys Glu Gln Pro Glu Tyr Asp Ser Thr Asp Gly Glu 
    290                 295                 300                 


Gly Asp Trp Ser Leu Trp Ser Val Cys Ser Val Thr Cys Gly Asn Gly 
305                 310                 315                 320 


Asn Gln Lys Arg Thr Arg Ser Cys Gly Tyr Ala Cys Ile Ala Thr Glu 
                325                 330                 335     


Ser Arg Thr Cys Asp Arg Pro Asn Cys Pro Gly Ile Glu Asp Thr Phe 
            340                 345                 350         


Arg Thr Ala Ala Thr Glu Val Ser Leu Leu Ala Gly Ser Glu Glu Phe 
        355                 360                 365             


Asn Ala Thr Lys Leu Phe Glu Val Asp Met Asp Ser Cys Glu Arg Trp 
    370                 375                 380                 


Met Ser Cys Lys Ser Glu Phe Leu Lys Lys Tyr Met His Lys Val Ile 
385                 390                 395                 400 


Asn Asp Leu Pro Ser Cys Pro Cys Ser Tyr Pro Thr Glu Val Ala Tyr 
                405                 410                 415     


Ser Thr Ala Asp Ile Phe Asp Arg Ile Lys Arg Lys Asp Phe Arg Trp 
            420                 425                 430         


Lys Asp Ala Ser Gly Pro Lys Glu Lys Leu Glu Ile Tyr Lys Pro Thr 
        435                 440                 445             


Ala Arg Tyr Cys Ile Arg Ser Met Leu Ser Leu Glu Ser Thr Thr Leu 
    450                 455                 460                 


Ala Ala Gln His Cys Cys Tyr Gly Asp Asn Met Gln Leu Ile Thr Arg 
465                 470                 475                 480 


Gly Lys Gly Ala Gly Thr Pro Asn Leu Ile Ser Thr Glu Phe Ser Ala 
                485                 490                 495     


Glu Leu His Tyr Lys Val Asp Val Leu Pro Trp Ile Ile Cys Lys Gly 
            500                 505                 510         


Asp Trp Ser Arg Tyr Asn Glu Ala Arg Pro Pro Asn Asn Gly Gln Lys 
        515                 520                 525             


Cys Thr Glu Ser Pro Ser Asp Glu Asp Tyr Ile Lys Gln Phe Gln Glu 
    530                 535                 540                 


Ala Arg Glu Tyr 
545             


<210>  29
<211>  287
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  bacISM1287-461

<400>  29

Met Gly Ser Ser His His His His His His Ser Gln Gly Ser Met Ser 
1               5                   10                  15      


Asp Ser Glu Val Asn Gln Glu Ala Lys Pro Glu Val Lys Pro Glu Val 
            20                  25                  30          


Lys Pro Glu Thr His Ile Asn Leu Lys Val Ser Asp Gly Ser Ser Glu 
        35                  40                  45              


Ile Phe Phe Lys Ile Lys Lys Thr Thr Pro Leu Arg Arg Leu Met Glu 
    50                  55                  60                  


Ala Phe Ala Lys Arg Gln Gly Lys Glu Met Asp Ser Leu Arg Phe Leu 
65                  70                  75                  80  


Tyr Asp Gly Ile Arg Ile Gln Ala Asp Gln Thr Pro Glu Asp Leu Asp 
                85                  90                  95      


Met Glu Asp Asn Asp Ile Ile Glu Ala His Arg Glu Gln Ile Gly Gly 
            100                 105                 110         


Phe Glu Val Asp Met Asp Ser Cys Glu Arg Trp Met Ser Cys Lys Ser 
        115                 120                 125             


Glu Phe Leu Lys Lys Tyr Met His Lys Val Ile Asn Asp Leu Pro Ser 
    130                 135                 140                 


Cys Pro Cys Ser Tyr Pro Thr Glu Val Ala Tyr Ser Thr Ala Asp Ile 
145                 150                 155                 160 


Phe Asp Arg Ile Lys Arg Lys Asp Phe Arg Trp Lys Asp Ala Ser Gly 
                165                 170                 175     


Pro Lys Glu Lys Leu Glu Ile Tyr Lys Pro Thr Ala Arg Tyr Cys Ile 
            180                 185                 190         


Arg Ser Met Leu Ser Leu Glu Ser Thr Thr Leu Ala Ala Gln His Cys 
        195                 200                 205             


Cys Tyr Gly Asp Asn Met Gln Leu Ile Thr Arg Gly Lys Gly Ala Gly 
    210                 215                 220                 


Thr Pro Asn Leu Ile Ser Thr Glu Phe Ser Ala Glu Leu His Tyr Lys 
225                 230                 235                 240 


Val Asp Val Leu Pro Trp Ile Ile Cys Lys Gly Asp Trp Ser Arg Tyr 
                245                 250                 255     


Asn Glu Ala Arg Pro Pro Asn Asn Gly Gln Lys Cys Thr Glu Ser Pro 
            260                 265                 270         


Ser Asp Glu Asp Tyr Ile Lys Gln Phe Gln Glu Ala Arg Glu Tyr 
        275                 280                 285         


<210>  30
<211>  480
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mamISM126-461

<400>  30

Asp Ala Ala Gln Pro Ala Arg Arg Ala Arg Arg Thr Lys Leu Gly Thr 
1               5                   10                  15      


Glu Leu Gly Ser Gly Ala Ser Asp Arg Gln Asp Ala Ala Ala Gly Asn 
            20                  25                  30          


Val Ser Gly Ser Gln Leu Gln Asn Asn Leu Asn Leu Glu Ser Asp Ser 
        35                  40                  45              


Thr Ser Glu Thr Ser Phe Pro Leu Ser Lys Glu Ala Pro Glu Glu His 
    50                  55                  60                  


Gln Val Val His Gln Pro Phe Pro Arg Gln Arg Phe Pro Pro Glu Thr 
65                  70                  75                  80  


Gly His Pro Ser Leu Gln Arg Asp Gly Pro Arg Ser Phe Leu Leu Asp 
                85                  90                  95      


Leu Pro Asn Phe Pro Asp Leu Ser Lys Ala Asp Ile Asn Gly Gln Asn 
            100                 105                 110         


Pro Asn Ile Gln Val Thr Ile Glu Val Val Asp Gly Pro Asp Ser Glu 
        115                 120                 125             


Ala Glu Lys Asp Gln His Pro Glu Asn Lys Pro Ser Trp Ser Leu Pro 
    130                 135                 140                 


Ala Pro Asp Trp Arg Ala Trp Trp Gln Arg Ser Leu Ser Leu Ala Arg 
145                 150                 155                 160 


Thr Asn Ser Gly Asp Gln Asp Asp Lys Tyr Asp Ser Thr Ser Asp Asp 
                165                 170                 175     


Ser Asn Phe Leu Ser Val Pro Arg Gly Trp Asp Arg Pro Ala Pro Gly 
            180                 185                 190         


His Arg Thr Phe Glu Thr Lys Glu Gln Pro Glu Tyr Asp Ser Thr Asp 
        195                 200                 205             


Gly Glu Gly Asp Trp Ser Leu Trp Ser Val Cys Ser Val Thr Cys Gly 
    210                 215                 220                 


Asn Gly Asn Gln Lys Arg Thr Arg Ser Cys Gly Tyr Ala Cys Ile Ala 
225                 230                 235                 240 


Thr Glu Ser Arg Thr Cys Asp Arg Pro Asn Cys Pro Gly Ile Glu Asp 
                245                 250                 255     


Thr Phe Arg Thr Ala Ala Thr Glu Val Ser Leu Leu Ala Gly Ser Glu 
            260                 265                 270         


Glu Phe Asn Ala Thr Lys Leu Phe Glu Val Asp Met Asp Ser Cys Glu 
        275                 280                 285             


Arg Trp Met Ser Cys Lys Ser Glu Phe Leu Lys Lys Tyr Met His Lys 
    290                 295                 300                 


Val Ile Asn Asp Leu Pro Ser Cys Pro Cys Ser Tyr Pro Thr Glu Val 
305                 310                 315                 320 


Ala Tyr Ser Thr Ala Asp Ile Phe Asp Arg Ile Lys Arg Lys Asp Phe 
                325                 330                 335     


Arg Trp Lys Asp Ala Ser Gly Pro Lys Glu Lys Leu Glu Ile Tyr Lys 
            340                 345                 350         


Pro Thr Ala Arg Tyr Cys Ile Arg Ser Met Leu Ser Leu Glu Ser Thr 
        355                 360                 365             


Thr Leu Ala Ala Gln His Cys Cys Tyr Gly Asp Asn Met Gln Leu Ile 
    370                 375                 380                 


Thr Arg Gly Lys Gly Ala Gly Thr Pro Asn Leu Ile Ser Thr Glu Phe 
385                 390                 395                 400 


Ser Ala Glu Leu His Tyr Lys Val Asp Val Leu Pro Trp Ile Ile Cys 
                405                 410                 415     


Lys Gly Asp Trp Ser Arg Tyr Asn Glu Ala Arg Pro Pro Asn Asn Gly 
            420                 425                 430         


Gln Lys Cys Thr Glu Ser Pro Ser Asp Glu Asp Tyr Ile Lys Gln Phe 
        435                 440                 445             


Gln Glu Ala Arg Glu Tyr Pro Arg Gly Gly Pro Glu Gln Lys Leu Ile 
    450                 455                 460                 


Ser Glu Glu Asp Leu Asn Ser Ala Val Asp His His His His His His 
465                 470                 475                 480 


<210>  31
<211>  221
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mamISM1287-461

<400>  31

Asp Ala Ala Gln Pro Ala Arg Arg Ala Arg Arg Thr Lys Leu Gly Thr 
1               5                   10                  15      


Glu Leu Gly Ser Phe Glu Val Asp Met Asp Ser Cys Glu Arg Trp Met 
            20                  25                  30          


Ser Cys Lys Ser Glu Phe Leu Lys Lys Tyr Met His Lys Val Ile Asn 
        35                  40                  45              


Asp Leu Pro Ser Cys Pro Cys Ser Tyr Pro Thr Glu Val Ala Tyr Ser 
    50                  55                  60                  


Thr Ala Asp Ile Phe Asp Arg Ile Lys Arg Lys Asp Phe Arg Trp Lys 
65                  70                  75                  80  


Asp Ala Ser Gly Pro Lys Glu Lys Leu Glu Ile Tyr Lys Pro Thr Ala 
                85                  90                  95      


Arg Tyr Cys Ile Arg Ser Met Leu Ser Leu Glu Ser Thr Thr Leu Ala 
            100                 105                 110         


Ala Gln His Cys Cys Tyr Gly Asp Asn Met Gln Leu Ile Thr Arg Gly 
        115                 120                 125             


Lys Gly Ala Gly Thr Pro Asn Leu Ile Ser Thr Glu Phe Ser Ala Glu 
    130                 135                 140                 


Leu His Tyr Lys Val Asp Val Leu Pro Trp Ile Ile Cys Lys Gly Asp 
145                 150                 155                 160 


Trp Ser Arg Tyr Asn Glu Ala Arg Pro Pro Asn Asn Gly Gln Lys Cys 
                165                 170                 175     


Thr Glu Ser Pro Ser Asp Glu Asp Tyr Ile Lys Gln Phe Gln Glu Ala 
            180                 185                 190         


Arg Glu Tyr Pro Arg Gly Gly Pro Glu Gln Lys Leu Ile Ser Glu Glu 
        195                 200                 205             


Asp Leu Asn Ser Ala Val Asp His His His His His His 
    210                 215                 220     


<210>  32
<211>  483
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mamISM126-464

<400>  32

Asp Ala Ala Gln Pro Ala Arg Arg Ala Arg Arg Thr Lys Leu Gly Thr 
1               5                   10                  15      


Glu Leu Gly Ser Gly Ala Ala Asp Gly Pro Asp Ala Ala Ala Gly Asn 
            20                  25                  30          


Ala Ser Gln Ala Gln Leu Gln Asn Asn Leu Asn Val Gly Ser Asp Thr 
        35                  40                  45              


Thr Ser Glu Thr Ser Phe Ser Leu Ser Lys Glu Ala Pro Arg Glu His 
    50                  55                  60                  


Leu Asp His Gln Ala Ala His Gln Pro Phe Pro Arg Pro Arg Phe Arg 
65                  70                  75                  80  


Gln Glu Thr Gly His Pro Ser Leu Gln Arg Asp Phe Pro Arg Ser Phe 
                85                  90                  95      


Leu Leu Asp Leu Pro Asn Phe Pro Asp Leu Ser Lys Ala Asp Ile Asn 
            100                 105                 110         


Gly Gln Asn Pro Asn Ile Gln Val Thr Ile Glu Val Val Asp Gly Pro 
        115                 120                 125             


Asp Ser Glu Ala Asp Lys Asp Gln His Pro Glu Asn Lys Pro Ser Trp 
    130                 135                 140                 


Ser Val Pro Ser Pro Asp Trp Arg Ala Trp Trp Gln Arg Ser Leu Ser 
145                 150                 155                 160 


Leu Ala Arg Ala Asn Ser Gly Asp Gln Asp Tyr Lys Tyr Asp Ser Thr 
                165                 170                 175     


Ser Asp Asp Ser Asn Phe Leu Asn Pro Pro Arg Gly Trp Asp His Thr 
            180                 185                 190         


Ala Pro Gly His Arg Thr Phe Glu Thr Lys Asp Gln Pro Glu Tyr Asp 
        195                 200                 205             


Ser Thr Asp Gly Glu Gly Asp Trp Ser Leu Trp Ser Val Cys Ser Val 
    210                 215                 220                 


Thr Cys Gly Asn Gly Asn Gln Lys Arg Thr Arg Ser Cys Gly Tyr Ala 
225                 230                 235                 240 


Cys Thr Ala Thr Glu Ser Arg Thr Cys Asp Arg Pro Asn Cys Pro Gly 
                245                 250                 255     


Ile Glu Asp Thr Phe Arg Thr Ala Ala Thr Glu Val Ser Leu Leu Ala 
            260                 265                 270         


Gly Ser Glu Glu Phe Asn Ala Thr Lys Leu Phe Glu Val Asp Thr Asp 
        275                 280                 285             


Ser Cys Glu Arg Trp Met Ser Cys Lys Ser Glu Phe Leu Lys Lys Tyr 
    290                 295                 300                 


Met His Lys Val Met Asn Asp Leu Pro Ser Cys Pro Cys Ser Tyr Pro 
305                 310                 315                 320 


Thr Glu Val Ala Tyr Ser Thr Ala Asp Ile Phe Asp Arg Ile Lys Arg 
                325                 330                 335     


Lys Asp Phe Arg Trp Lys Asp Ala Ser Gly Pro Lys Glu Lys Leu Glu 
            340                 345                 350         


Ile Tyr Lys Pro Thr Ala Arg Tyr Cys Ile Arg Ser Met Leu Ser Leu 
        355                 360                 365             


Glu Ser Thr Thr Leu Ala Ala Gln His Cys Cys Tyr Gly Asp Asn Met 
    370                 375                 380                 


Gln Leu Ile Thr Arg Gly Lys Gly Ala Gly Thr Pro Asn Leu Ile Ser 
385                 390                 395                 400 


Thr Glu Phe Ser Ala Glu Leu His Tyr Lys Val Asp Val Leu Pro Trp 
                405                 410                 415     


Ile Ile Cys Lys Gly Asp Trp Ser Arg Tyr Asn Glu Ala Arg Pro Pro 
            420                 425                 430         


Asn Asn Gly Gln Lys Cys Thr Glu Ser Pro Ser Asp Glu Asp Tyr Ile 
        435                 440                 445             


Lys Gln Phe Gln Glu Ala Arg Glu Tyr Pro Arg Gly Gly Pro Glu Gln 
    450                 455                 460                 


Lys Leu Ile Ser Glu Glu Asp Leu Asn Ser Ala Val Asp His His His 
465                 470                 475                 480 


His His His 
            


<210>  33
<211>  559
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  bacISM126-464

<400>  33

Met Gly Ser Ser His His His His His His Ser Gln Gly Ser Met Ser 
1               5                   10                  15      


Asp Ser Glu Val Asn Gln Glu Ala Lys Pro Glu Val Lys Pro Glu Val 
            20                  25                  30          


Lys Pro Glu Thr His Ile Asn Leu Lys Val Ser Asp Gly Ser Ser Glu 
        35                  40                  45              


Ile Phe Phe Lys Ile Lys Lys Thr Thr Pro Leu Arg Arg Leu Met Glu 
    50                  55                  60                  


Ala Phe Ala Lys Arg Gln Gly Lys Glu Met Asp Ser Leu Arg Phe Leu 
65                  70                  75                  80  


Tyr Asp Gly Ile Arg Ile Gln Ala Asp Gln Thr Pro Glu Asp Leu Asp 
                85                  90                  95      


Met Glu Asp Asn Asp Ile Ile Glu Ala His Arg Glu Gln Ile Gly Gly 
            100                 105                 110         


Gly Ser Gly Ala Ala Asp Gly Pro Asp Ala Ala Ala Gly Asn Ala Ser 
        115                 120                 125             


Gln Ala Gln Leu Gln Asn Asn Leu Asn Val Gly Ser Asp Thr Thr Ser 
    130                 135                 140                 


Glu Thr Ser Phe Ser Leu Ser Lys Glu Ala Pro Arg Glu His Leu Asp 
145                 150                 155                 160 


His Gln Ala Ala His Gln Pro Phe Pro Arg Pro Arg Phe Arg Gln Glu 
                165                 170                 175     


Thr Gly His Pro Ser Leu Gln Arg Asp Phe Pro Arg Ser Phe Leu Leu 
            180                 185                 190         


Asp Leu Pro Asn Phe Pro Asp Leu Ser Lys Ala Asp Ile Asn Gly Gln 
        195                 200                 205             


Asn Pro Asn Ile Gln Val Thr Ile Glu Val Val Asp Gly Pro Asp Ser 
    210                 215                 220                 


Glu Ala Asp Lys Asp Gln His Pro Glu Asn Lys Pro Ser Trp Ser Val 
225                 230                 235                 240 


Pro Ser Pro Asp Trp Arg Ala Trp Trp Gln Arg Ser Leu Ser Leu Ala 
                245                 250                 255     


Arg Ala Asn Ser Gly Asp Gln Asp Tyr Lys Tyr Asp Ser Thr Ser Asp 
            260                 265                 270         


Asp Ser Asn Phe Leu Asn Pro Pro Arg Gly Trp Asp His Thr Ala Pro 
        275                 280                 285             


Gly His Arg Thr Phe Glu Thr Lys Asp Gln Pro Glu Tyr Asp Ser Thr 
    290                 295                 300                 


Asp Gly Glu Gly Asp Trp Ser Leu Trp Ser Val Cys Ser Val Thr Cys 
305                 310                 315                 320 


Gly Asn Gly Asn Gln Lys Arg Thr Arg Ser Cys Gly Tyr Ala Cys Thr 
                325                 330                 335     


Ala Thr Glu Ser Arg Thr Cys Asp Arg Pro Asn Cys Pro Gly Ile Glu 
            340                 345                 350         


Asp Thr Phe Arg Thr Ala Ala Thr Glu Val Ser Leu Leu Ala Gly Ser 
        355                 360                 365             


Glu Glu Phe Asn Ala Thr Lys Leu Phe Glu Val Asp Thr Asp Ser Cys 
    370                 375                 380                 


Glu Arg Trp Met Ser Cys Lys Ser Glu Phe Leu Lys Lys Tyr Met His 
385                 390                 395                 400 


Lys Val Met Asn Asp Leu Pro Ser Cys Pro Cys Ser Tyr Pro Thr Glu 
                405                 410                 415     


Val Ala Tyr Ser Thr Ala Asp Ile Phe Asp Arg Ile Lys Arg Lys Asp 
            420                 425                 430         


Phe Arg Trp Lys Asp Ala Ser Gly Pro Lys Glu Lys Leu Glu Ile Tyr 
        435                 440                 445             


Lys Pro Thr Ala Arg Tyr Cys Ile Arg Ser Met Leu Ser Leu Glu Ser 
    450                 455                 460                 


Thr Thr Leu Ala Ala Gln His Cys Cys Tyr Gly Asp Asn Met Gln Leu 
465                 470                 475                 480 


Ile Thr Arg Gly Lys Gly Ala Gly Thr Pro Asn Leu Ile Ser Thr Glu 
                485                 490                 495     


Phe Ser Ala Glu Leu His Tyr Lys Val Asp Val Leu Pro Trp Ile Ile 
            500                 505                 510         


Cys Lys Gly Asp Trp Ser Arg Tyr Asn Glu Ala Arg Pro Pro Asn Asn 
        515                 520                 525             


Gly Gln Lys Cys Thr Glu Ser Pro Ser Asp Glu Asp Tyr Ile Lys Gln 
    530                 535                 540                 


Phe Gln Glu Ala Arg Glu Tyr Asp Tyr Lys Asp Asp Asp Asp Lys 
545                 550                 555                 


<210>  34
<211>  295
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  bacISM1290-464

<400>  34

Met Gly Ser Ser His His His His His His Ser Gln Gly Ser Met Ser 
1               5                   10                  15      


Asp Ser Glu Val Asn Gln Glu Ala Lys Pro Glu Val Lys Pro Glu Val 
            20                  25                  30          


Lys Pro Glu Thr His Ile Asn Leu Lys Val Ser Asp Gly Ser Ser Glu 
        35                  40                  45              


Ile Phe Phe Lys Ile Lys Lys Thr Thr Pro Leu Arg Arg Leu Met Glu 
    50                  55                  60                  


Ala Phe Ala Lys Arg Gln Gly Lys Glu Met Asp Ser Leu Arg Phe Leu 
65                  70                  75                  80  


Tyr Asp Gly Ile Arg Ile Gln Ala Asp Gln Thr Pro Glu Asp Leu Asp 
                85                  90                  95      


Met Glu Asp Asn Asp Ile Ile Glu Ala His Arg Glu Gln Ile Gly Gly 
            100                 105                 110         


Phe Glu Val Asp Thr Asp Ser Cys Glu Arg Trp Met Ser Cys Lys Ser 
        115                 120                 125             


Glu Phe Leu Lys Lys Tyr Met His Lys Val Met Asn Asp Leu Pro Ser 
    130                 135                 140                 


Cys Pro Cys Ser Tyr Pro Thr Glu Val Ala Tyr Ser Thr Ala Asp Ile 
145                 150                 155                 160 


Phe Asp Arg Ile Lys Arg Lys Asp Phe Arg Trp Lys Asp Ala Ser Gly 
                165                 170                 175     


Pro Lys Glu Lys Leu Glu Ile Tyr Lys Pro Thr Ala Arg Tyr Cys Ile 
            180                 185                 190         


Arg Ser Met Leu Ser Leu Glu Ser Thr Thr Leu Ala Ala Gln His Cys 
        195                 200                 205             


Cys Tyr Gly Asp Asn Met Gln Leu Ile Thr Arg Gly Lys Gly Ala Gly 
    210                 215                 220                 


Thr Pro Asn Leu Ile Ser Thr Glu Phe Ser Ala Glu Leu His Tyr Lys 
225                 230                 235                 240 


Val Asp Val Leu Pro Trp Ile Ile Cys Lys Gly Asp Trp Ser Arg Tyr 
                245                 250                 255     


Asn Glu Ala Arg Pro Pro Asn Asn Gly Gln Lys Cys Thr Glu Ser Pro 
            260                 265                 270         


Ser Asp Glu Asp Tyr Ile Lys Gln Phe Gln Glu Ala Arg Glu Tyr Asp 
        275                 280                 285             


Tyr Lys Asp Asp Asp Asp Lys 
    290                 295 


<210>  35
<211>  221
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mamISM1 290-464

<400>  35

Asp Ala Ala Gln Pro Ala Arg Arg Ala Arg Arg Thr Lys Leu Gly Thr 
1               5                   10                  15      


Glu Leu Gly Ser Phe Glu Val Asp Thr Asp Ser Cys Glu Arg Trp Met 
            20                  25                  30          


Ser Cys Lys Ser Glu Phe Leu Lys Lys Tyr Met His Lys Val Met Asn 
        35                  40                  45              


Asp Leu Pro Ser Cys Pro Cys Ser Tyr Pro Thr Glu Val Ala Tyr Ser 
    50                  55                  60                  


Thr Ala Asp Ile Phe Asp Arg Ile Lys Arg Lys Asp Phe Arg Trp Lys 
65                  70                  75                  80  


Asp Ala Ser Gly Pro Lys Glu Lys Leu Glu Ile Tyr Lys Pro Thr Ala 
                85                  90                  95      


Arg Tyr Cys Ile Arg Ser Met Leu Ser Leu Glu Ser Thr Thr Leu Ala 
            100                 105                 110         


Ala Gln His Cys Cys Tyr Gly Asp Asn Met Gln Leu Ile Thr Arg Gly 
        115                 120                 125             


Lys Gly Ala Gly Thr Pro Asn Leu Ile Ser Thr Glu Phe Ser Ala Glu 
    130                 135                 140                 


Leu His Tyr Lys Val Asp Val Leu Pro Trp Ile Ile Cys Lys Gly Asp 
145                 150                 155                 160 


Trp Ser Arg Tyr Asn Glu Ala Arg Pro Pro Asn Asn Gly Gln Lys Cys 
                165                 170                 175     


Thr Glu Ser Pro Ser Asp Glu Asp Tyr Ile Lys Gln Phe Gln Glu Ala 
            180                 185                 190         


Arg Glu Tyr Pro Arg Gly Gly Pro Glu Gln Lys Leu Ile Ser Glu Glu 
        195                 200                 205             


Asp Leu Asn Ser Ala Val Asp His His His His His His 
    210                 215                 220     

