                         SEQUENCE LISTING

<110>  National University of Singapore
 
<120>  Engineered Immune Cells

<130>  S61014169

<160>  43    

<170>  PatentIn version 3.5

<210>  1
<211>  41
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Intracellular signalling domain

<400>  1

Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr 
1               5                   10                  15      


Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro 
            20                  25                  30          


Pro Arg Asp Phe Ala Ala Tyr Arg Ser 
        35                  40      


<210>  2
<211>  242
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Anti-CD19 scFV domain

<400>  2

Asp Ile Gln Met Thr Gln Thr Thr Ser Ser Leu Ser Ala Ser Leu Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Ser Cys Arg Ala Ser Gln Asp Ile Ser Lys Tyr 
            20                  25                  30          


Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly Thr Val Lys Leu Leu Ile 
        35                  40                  45              


Tyr His Thr Ser Arg Leu His Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile Ser Asn Leu Glu Gln 
65                  70                  75                  80  


Glu Asp Ile Ala Thr Tyr Phe Cys Gln Gln Gly Asn Thr Leu Pro Tyr 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Thr Gly Gly Gly Gly Ser 
            100                 105                 110         


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Val Lys Leu Gln Glu 
        115                 120                 125             


Ser Gly Pro Gly Leu Val Ala Pro Ser Gln Ser Leu Ser Val Thr Cys 
    130                 135                 140                 


Thr Val Ser Gly Val Ser Leu Pro Asp Tyr Gly Val Ser Trp Ile Arg 
145                 150                 155                 160 


Gln Pro Pro Arg Lys Gly Leu Glu Trp Leu Gly Val Ile Trp Gly Ser 
                165                 170                 175     


Glu Thr Thr Tyr Tyr Asn Ser Ala Leu Lys Ser Arg Leu Thr Ile Ile 
            180                 185                 190         


Lys Asp Asn Ser Lys Ser Gln Val Phe Leu Lys Met Asn Ser Leu Gln 
        195                 200                 205             


Thr Asp Asp Thr Ala Ile Tyr Tyr Cys Ala Lys His Tyr Tyr Tyr Gly 
    210                 215                 220                 


Gly Ser Tyr Ala Met Asp Tyr Trp Gly Gln Gly Thr Ser Val Thr Val 
225                 230                 235                 240 


Ser Ser 
        


<210>  3
<211>  240
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  scFV capable of binding to LMP2A peptide - MHC complex

<400>  3

Gln Val Gln Leu Lys Gln Ser Gly Pro Gly Leu Val Gln Pro Ser Gln 
1               5                   10                  15      


Ser Leu Ser Ile Thr Cys Thr Val Ser Gly Phe Ser Leu Thr Asn Tyr 
            20                  25                  30          


Gly Val His Trp Val Arg Gln Ser Pro Gly Lys Gly Leu Glu Trp Leu 
        35                  40                  45              


Gly Val Ile Trp Ser Gly Gly Ser Thr Asp Tyr Asn Ala Ala Phe Ile 
    50                  55                  60                  


Ser Arg Leu Ser Ile Ser Lys Asp Asn Ser Lys Ser Gln Val Phe Phe 
65                  70                  75                  80  


Lys Met Asn Ser Leu Gln Ala Asn Asp Thr Ala Ile Tyr Tyr Cys Ala 
                85                  90                  95      


Arg Asn Trp Val Pro Tyr Tyr Phe Asp Tyr Trp Gly Gln Gly Thr Thr 
            100                 105                 110         


Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
        115                 120                 125             


Gly Gly Gly Ser Thr Asp Ile Val Met Thr Gln Ser Gln Lys Phe Met 
    130                 135                 140                 


Ser Thr Ser Val Gly Asp Arg Val Ser Val Thr Cys Arg Ala Ser Gln 
145                 150                 155                 160 


Asn Val Phe Thr Asn Val Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ala 
                165                 170                 175     


Pro Lys Ala Leu Ile Tyr Ser Thr Ser Tyr Arg Tyr Ser Gly Val Pro 
            180                 185                 190         


Asp Arg Phe Thr Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile 
        195                 200                 205             


Ser Asn Val Gln Ser Glu Asp Leu Ala Glu Tyr Phe Cys Gln Gln Tyr 
    210                 215                 220                 


Ile Ser Tyr Pro Leu Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu Lys 
225                 230                 235                 240 


<210>  4
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  signal peptide

<400>  4

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro 
            20      


<210>  5
<211>  1563
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD28-CAR

<400>  5
atggccttac cagtgaccgc cttgctcctg ccgctggcct tgctgctcca cgccgccagg       60

ccggacatcc agatgaccca gaccacaagc tccctgtctg ccagcctggg cgacagggtg      120

acaatctctt gccgcgccag ccaggacatc tccaagtacc tgaactggta tcagcagaag      180

cctgacggca ccgtgaagct gctgatctac cacacatctc ggctgcacag cggcgtgcca      240

tccaggttct ccggctctgg cagcggcacc gactattctc tgacaatcag caacctggag      300

caggaggaca tcgccaccta cttctgccag cagggcaata ccctgcccta tacatttggc      360

ggcggcacca agctggagat cacaggagga ggaggcagcg gcggaggagg ctccggcggc      420

ggcggctctg aggtgaagct gcaggagtcc ggaccaggcc tggtggcacc atcccagtct      480

ctgagcgtga cctgtacagt gagcggcgtg tccctgcctg actacggcgt gtcctggatc      540

agacagcccc ctagaaaggg cctggagtgg ctgggcgtga tctggggcag cgagacaaca      600

tactataatt ccgccctgaa gtctcggctg accatcatca aggataactc caagtctcag      660

gtgtttctga agatgaatag cctgcagacc gacgatacag ccatctacta ttgtgccaag      720

cactactatt acggggggag ttatgctatg gattattggg ggcagggcac cagcgtcact      780

gtctcatcac gggcggccgc acatcatcat caccaccatc acggggccgc agaacaaaaa      840

ctcatctcag aagaggatcg tacgaagccc accacgacgc cagcgccgcg accaccaaca      900

ccggcgccca ccatcgcgtc gcagcccctg tccctgcgcc cagaggcgtg ccggccagcg      960

gcggggggcg cagtacacac gagggggctg gacttcgcct gtgatacgcg tttttgggtg     1020

ctggtggtgg ttggtggagt cctggcttgc tatagcttgc tagtaacagt ggcctttatt     1080

attttctggg tgaggagtaa gaggagcagg ctcctgcaca gtgactacat gaacatgact     1140

ccccgccgcc ccgggcccac ccgcaagcat taccagccct atgccccacc acgcgacttc     1200

gcagcctatc gctccggatc cagagtgaag ttcagcagga gcgcagacgc ccccgcgtac     1260

cagcagggcc agaaccagct ctataacgag ctcaatctag gacgaagaga ggagtacgat     1320

gttttggaca agagacgtgg ccgggaccct gagatggggg gaaagccgca gagaaggaag     1380

aaccctcagg aaggcctgta caatgaactg cagaaagata agatggcgga ggcctacagt     1440

gagattggga tgaaaggcga gcgccggagg ggcaaggggc acgatggcct ttaccagggt     1500

ctcagtacag ccaccaagga cacctacgac gcccttcaca tgcaggccct gccccctcgc     1560

taa                                                                   1563


<210>  6
<211>  520
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD28 CAR

<400>  6

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Asp Ile Gln Met Thr Gln Thr Thr Ser Ser Leu 
            20                  25                  30          


Ser Ala Ser Leu Gly Asp Arg Val Thr Ile Ser Cys Arg Ala Ser Gln 
        35                  40                  45              


Asp Ile Ser Lys Tyr Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly Thr 
    50                  55                  60                  


Val Lys Leu Leu Ile Tyr His Thr Ser Arg Leu His Ser Gly Val Pro 
65                  70                  75                  80  


Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile 
                85                  90                  95      


Ser Asn Leu Glu Gln Glu Asp Ile Ala Thr Tyr Phe Cys Gln Gln Gly 
            100                 105                 110         


Asn Thr Leu Pro Tyr Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Thr 
        115                 120                 125             


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu 
    130                 135                 140                 


Val Lys Leu Gln Glu Ser Gly Pro Gly Leu Val Ala Pro Ser Gln Ser 
145                 150                 155                 160 


Leu Ser Val Thr Cys Thr Val Ser Gly Val Ser Leu Pro Asp Tyr Gly 
                165                 170                 175     


Val Ser Trp Ile Arg Gln Pro Pro Arg Lys Gly Leu Glu Trp Leu Gly 
            180                 185                 190         


Val Ile Trp Gly Ser Glu Thr Thr Tyr Tyr Asn Ser Ala Leu Lys Ser 
        195                 200                 205             


Arg Leu Thr Ile Ile Lys Asp Asn Ser Lys Ser Gln Val Phe Leu Lys 
    210                 215                 220                 


Met Asn Ser Leu Gln Thr Asp Asp Thr Ala Ile Tyr Tyr Cys Ala Lys 
225                 230                 235                 240 


His Tyr Tyr Tyr Gly Gly Ser Tyr Ala Met Asp Tyr Trp Gly Gln Gly 
                245                 250                 255     


Thr Ser Val Thr Val Ser Ser Arg Ala Ala Ala His His His His His 
            260                 265                 270         


His His Gly Ala Ala Glu Gln Lys Leu Ile Ser Glu Glu Asp Arg Thr 
        275                 280                 285             


Lys Pro Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr 
    290                 295                 300                 


Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala 
305                 310                 315                 320 


Ala Gly Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp Thr 
                325                 330                 335     


Arg Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser 
            340                 345                 350         


Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val Arg Ser Lys Arg 
        355                 360                 365             


Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr Pro Arg Arg Pro 
    370                 375                 380                 


Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro Pro Arg Asp Phe 
385                 390                 395                 400 


Ala Ala Tyr Arg Ser Gly Ser Arg Val Lys Phe Ser Arg Ser Ala Asp 
                405                 410                 415     


Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn 
            420                 425                 430         


Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg 
        435                 440                 445             


Asp Pro Glu Met Gly Gly Lys Pro Gln Arg Arg Lys Asn Pro Gln Glu 
    450                 455                 460                 


Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser 
465                 470                 475                 480 


Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly 
                485                 490                 495     


Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu 
            500                 505                 510         


His Met Gln Ala Leu Pro Pro Arg 
        515                 520 


<210>  7
<211>  1689
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD137/CD28-CAR

<400>  7
atggccttac cagtgaccgc cttgctcctg ccgctggcct tgctgctcca cgccgccagg       60

ccgcaggtgc agctgaagca gtcaggacct ggcctagtgc agccctcaca gagcctgtcc      120

atcacctgca cagtctctgg tttctcatta actaactatg gtgtacactg ggttcgccag      180

tctccaggaa agggtctgga gtggctggga gtgatatgga gtggtggaag cacagactat      240

aatgcagctt tcatatccag attgagcatc agcaaggaca attccaagag ccaagttttc      300

tttaaaatga acagtctgca agctaatgac acagccatat attactgtgc cagaaattgg      360

gtcccttact actttgacta ctggggccaa ggcaccacgg tcaccgtatc gagcggtgga      420

ggcggttcag gcggaggtgg cagcggcggt ggcgggtcga cggacattgt gatgacccag      480

tctcaaaaat tcatgtccac atcagttgga gacagggtca gcgtcacctg cagggccagt      540

cagaatgtgt ttactaatgt agcctggtat caacagaaac cagggcaagc tcctaaagca      600

ctgatttact cgacatccta ccggtacagt ggagtccctg atcgcttcac aggcagtgga      660

tctgggacag atttcactct caccatcagc aatgtgcagt ctgaagactt ggcagagtat      720

ttctgtcagc aatatatcag ctatcctctc acgttcggtg ctgggaccaa gctggagctg      780

aaacgggcgg ccgcacatca tcatcaccac catcacgggg ccgcagaaca aaaactcatc      840

tcagaagagg atcgtacgaa gcccaccacg acgccagcgc cgcgaccacc aacaccggcg      900

cccaccatcg cgtcgcagcc cctgtccctg cgcccagagg cgtgccggcc agcggcgggg      960

ggcgcagtac acacgagggg gctggacttc gcctgtgata cgcgtttttg ggtgctggtg     1020

gtggttggtg gagtcctggc ttgctatagc ttgctagtaa cagtggcctt tattattttc     1080

tgggtgagga gtaagaggag caggctcctg cacagtgact acatgaacat gactccccgc     1140

cgccccgggc ccacccgcaa gcattaccag ccctatgccc caccacgcga cttcgcagcc     1200

tatcgctccg gatccaaacg gggcagaaag aaactcctgt atatattcaa acaaccattt     1260

atgagaccag tacaaactac tcaagaggaa gatggctgta gctgccgatt tccagaagaa     1320

gaagaaggag gatgtgaact gggatccaga gtgaagttca gcaggagcgc agacgccccc     1380

gcgtaccagc agggccagaa ccagctctat aacgagctca atctaggacg aagagaggag     1440

tacgatgttt tggacaagag acgtggccgg gaccctgaga tggggggaaa gccgcagaga     1500

aggaagaacc ctcaggaagg cctgtacaat gaactgcaga aagataagat ggcggaggcc     1560

tacagtgaga ttgggatgaa aggcgagcgc cggaggggca aggggcacga tggcctttac     1620

cagggtctca gtacagccac caaggacacc tacgacgccc ttcacatgca ggccctgccc     1680

cctcgctaa                                                             1689


<210>  8
<211>  562
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD137/CD28-CAR

<400>  8

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Gln Val Gln Leu Lys Gln Ser Gly Pro Gly Leu 
            20                  25                  30          


Val Gln Pro Ser Gln Ser Leu Ser Ile Thr Cys Thr Val Ser Gly Phe 
        35                  40                  45              


Ser Leu Thr Asn Tyr Gly Val His Trp Val Arg Gln Ser Pro Gly Lys 
    50                  55                  60                  


Gly Leu Glu Trp Leu Gly Val Ile Trp Ser Gly Gly Ser Thr Asp Tyr 
65                  70                  75                  80  


Asn Ala Ala Phe Ile Ser Arg Leu Ser Ile Ser Lys Asp Asn Ser Lys 
                85                  90                  95      


Ser Gln Val Phe Phe Lys Met Asn Ser Leu Gln Ala Asn Asp Thr Ala 
            100                 105                 110         


Ile Tyr Tyr Cys Ala Arg Asn Trp Val Pro Tyr Tyr Phe Asp Tyr Trp 
        115                 120                 125             


Gly Gln Gly Thr Thr Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly 
    130                 135                 140                 


Gly Gly Gly Ser Gly Gly Gly Gly Ser Thr Asp Ile Val Met Thr Gln 
145                 150                 155                 160 


Ser Gln Lys Phe Met Ser Thr Ser Val Gly Asp Arg Val Ser Val Thr 
                165                 170                 175     


Cys Arg Ala Ser Gln Asn Val Phe Thr Asn Val Ala Trp Tyr Gln Gln 
            180                 185                 190         


Lys Pro Gly Gln Ala Pro Lys Ala Leu Ile Tyr Ser Thr Ser Tyr Arg 
        195                 200                 205             


Tyr Ser Gly Val Pro Asp Arg Phe Thr Gly Ser Gly Ser Gly Thr Asp 
    210                 215                 220                 


Phe Thr Leu Thr Ile Ser Asn Val Gln Ser Glu Asp Leu Ala Glu Tyr 
225                 230                 235                 240 


Phe Cys Gln Gln Tyr Ile Ser Tyr Pro Leu Thr Phe Gly Ala Gly Thr 
                245                 250                 255     


Lys Leu Glu Leu Lys Arg Ala Ala Ala His His His His His His His 
            260                 265                 270         


Gly Ala Ala Glu Gln Lys Leu Ile Ser Glu Glu Asp Arg Thr Lys Pro 
        275                 280                 285             


Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala 
    290                 295                 300                 


Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly 
305                 310                 315                 320 


Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp Thr Arg Phe 
                325                 330                 335     


Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu Leu 
            340                 345                 350         


Val Thr Val Ala Phe Ile Ile Phe Trp Val Arg Ser Lys Arg Ser Arg 
        355                 360                 365             


Leu Leu His Ser Asp Tyr Met Asn Met Thr Pro Arg Arg Pro Gly Pro 
    370                 375                 380                 


Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro Pro Arg Asp Phe Ala Ala 
385                 390                 395                 400 


Tyr Arg Ser Gly Ser Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe 
                405                 410                 415     


Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly 
            420                 425                 430         


Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Gly 
        435                 440                 445             


Ser Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln 
    450                 455                 460                 


Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu 
465                 470                 475                 480 


Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly 
                485                 490                 495     


Lys Pro Gln Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu 
            500                 505                 510         


Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly 
        515                 520                 525             


Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser 
    530                 535                 540                 


Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro 
545                 550                 555                 560 


Pro Arg 
        


<210>  9
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  LCK gRNA1

<400>  9
gacccactgg ttacctacga                                                   20


<210>  10
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  LCK gRNA2

<400>  10
gccgggaaaa gtgattcgag                                                   20


<210>  11
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  LCK gRNA3

<400>  11
cgggacttcg accagaacca                                                   20


<210>  12
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  LCK gRNA4

<400>  12
tggagcccga accgtaagtg                                                   20


<210>  13
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  LCK gRNA5

<400>  13
cggcggattg gagccttcgt                                                   20


<210>  14
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  LCK gRNA6

<400>  14
gcggaccagt tcatgcaggc                                                   20


<210>  15
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  LCK gRNA7

<400>  15
cgttgtagta ccctgggtcg                                                   20


<210>  16
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  LCK gRNA8

<400>  16
gggtgacaat ttccgtcagc                                                   20


<210>  17
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  LCK gRNA9

<400>  17
cgtaagtggg gacccgtcg                                                    19


<210>  18
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  LCK gRNA10

<400>  18
ctggagcccg aaccgtaagt                                                   20


<210>  19
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  LCK gRNA11

<400>  19
ggtgaatgtc ccgtagttaa                                                   20


<210>  20
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  LCK gRNA12

<400>  20
gatcgttttc actgtcggtc                                                   20


<210>  21
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  LCK gRNA13

<400>  21
ccgtaagtgg ggacccgtcg                                                   20


<210>  22
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  LCK gRNA14

<400>  22
ccctctcacg acggagatct                                                   20


<210>  23
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  LCK gRNA15

<400>  23
ctggcgcccg ggaacactca                                                   20


<210>  24
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  LCK gRNA16

<400>  24
gtaagtgggg acccgtcgtg                                                   20


<210>  25
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  LCK gRNA17

<400>  25
tgtgcccgtt gtagtaccct                                                   20


<210>  26
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  LCK gRNA18

<400>  26
attacaccag tgagcccgac                                                   20


<210>  27
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  LCK gRNA19

<400>  27
tggcctagca cgcctcattg                                                   20


<210>  28
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  LCK gRNA20

<400>  28
cgtagagccg aaccagccgc                                                   20


<210>  29
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  LCK gRNA21

<400>  29
atccgtaatc tggacaacgg                                                   20


<210>  30
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  LCK gRNA22

<400>  30
gccctctcac gacggagatc                                                   20


<210>  31
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  LCK gRNA23

<400>  31
atcgttttca ctgtcggtcc                                                   20


<210>  32
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  LCK gRNA24

<400>  32
gaacctgagc cgcaaggacg                                                   20


<210>  33
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  LCK gRNA25

<400>  33
ccattatccc atagtcccac                                                   20


<210>  34
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  LCK gRNA26

<400>  34
gctcacaccc ggaagatgac                                                   20


<210>  35
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  LCK gRNA27

<400>  35
ctcacggctc cttcctcatc                                                   20


<210>  36
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  LCK gRNA28

<400>  36
aaggcgcagt ccctgaccac                                                   20


<210>  37
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  LCK gRNA29

<400>  37
ctacgaaggc tccaatccgc                                                   20


<210>  38
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  FYN gRNA1

<400>  38
agagttcaca cctccaaaga                                                   20


<210>  39
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  FYN gRNA2

<400>  39
acggggacct tgcgtacgag                                                   20


<210>  40
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  FYN gRNA3

<400>  40
ttgtcctttg gaaacccaag                                                   20


<210>  41
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  FYN gRNA4

<400>  41
gtcccccgaa tcattccttg                                                   20


<210>  42
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  FYN gRNA5

<400>  42
tggatactac attaccaccc                                                   20


<210>  43
<211>  100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RNA sequence


<220>
<221>  misc_feature
<222>  (1)..(3)
<223>  modified base with 2'-O-methyl 3' phosphorothioate

<220>
<221>  misc_feature
<222>  (97)..(99)
<223>  modified base with 2'-O-methyl 3' phosphorothioate

<400>  43
gccgggaaaa gugauucgag guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc       60

cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu                            100


