                         SEQUENCE LISTING

<110>  CytoSeek Ltd
 
<120>  Product and method

<130>  P3479PC00

<150>  GB1902992.5
<151>  2019-03-06

<160>  15    

<170>  PatentIn version 3.5

<210>  1
<211>  166
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Charge-modified myoglobin

<400>  1

Met His His His His His His Gly Ser Ser Gly Glu Asn Leu Tyr Phe 
1               5                   10                  15      


Gln Gly Leu Ser Asp Gly Glu Trp Gln Leu Val Leu Asn Val Trp Gly 
            20                  25                  30          


Lys Val Glu Ala Asp Ile Pro Gly His Gly Gln Glu Val Leu Ile Arg 
        35                  40                  45              


Leu Phe Lys Gly His Pro Lys Thr Leu Lys Lys Phe Asp Arg Phe Lys 
    50                  55                  60                  


His Leu Lys Ser Lys Lys Lys Met Lys Ala Ser Glu Lys Leu Lys Lys 
65                  70                  75                  80  


His Gly Ala Thr Val Leu Thr Ala Leu Gly Gly Ile Leu Lys Lys Lys 
                85                  90                  95      


Gly His His Glu Ala Lys Ile Lys Pro Leu Ala Gln Ser His Ala Thr 
            100                 105                 110         


Lys His Lys Ile Pro Val Lys Tyr Leu Lys Phe Ile Ser Lys Ala Ile 
        115                 120                 125             


Ile Lys Val Leu Gln Ser Lys His Pro Gly Asp Phe Gly Lys Lys Ala 
    130                 135                 140                 


Gln Gly Ala Met Asn Lys Ala Leu Lys Leu Phe Arg Lys Lys Met Ala 
145                 150                 155                 160 


Ser Asn Tyr Lys Glu Leu 
                165     


<210>  2
<211>  166
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Charge-modified myoglobin

<400>  2

Met His His His His His His Gly Ser Ser Gly Glu Asn Leu Tyr Phe 
1               5                   10                  15      


Gln Gly Leu Ser Asp Gly Glu Trp Gln Leu Val Leu Asn Val Trp Gly 
            20                  25                  30          


Lys Val Glu Ala Lys Ile Pro Gly His Gly Gln Glu Val Leu Ile Arg 
        35                  40                  45              


Leu Phe Lys Gly His Pro Lys Thr Leu Lys Lys Phe Asp Arg Phe Lys 
    50                  55                  60                  


His Leu Lys Ser Lys Lys Lys Met Lys Ala Ser Lys Lys Leu Lys Lys 
65                  70                  75                  80  


His Gly Ala Thr Val Leu Thr Ala Leu Gly Gly Ile Leu Lys Lys Lys 
                85                  90                  95      


Gly His His Lys Ala Lys Ile Lys Pro Leu Ala Gln Ser His Ala Thr 
            100                 105                 110         


Lys His Lys Ile Pro Val Lys Tyr Leu Lys Phe Ile Ser Lys Ala Ile 
        115                 120                 125             


Ile Gln Val Leu Gln Ser Lys His Pro Gly Lys Phe Gly Ala Lys Ala 
    130                 135                 140                 


Gln Gly Ala Met Asn Lys Ala Leu Lys Leu Phe Arg Lys Lys Met Ala 
145                 150                 155                 160 


Ser Asn Tyr Lys Lys Leu 
                165     


<210>  3
<211>  166
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Charge-modified myoglobin

<400>  3

Met His His His His His His Gly Ser Ser Gly Glu Asn Leu Tyr Phe 
1               5                   10                  15      


Gln Gly Leu Ser Lys Gly Lys Trp Gln Leu Val Leu Asn Val Trp Gly 
            20                  25                  30          


Lys Val Lys Ala Lys Ile Pro Gly His Gly Gln Lys Val Leu Ile Arg 
        35                  40                  45              


Leu Phe Lys Gly His Pro Lys Thr Leu Lys Glu Phe Lys Arg Phe Lys 
    50                  55                  60                  


His Leu Lys Ser Lys Lys Lys Met Lys Ala Ser Lys Lys Leu Lys Lys 
65                  70                  75                  80  


His Gly Ala Thr Val Leu Thr Ala Leu Gly Gly Ile Leu Lys Lys Lys 
                85                  90                  95      


Gly His His Glu Ala Lys Ile Glu Pro Leu Ala Gln Ser His Ala Thr 
            100                 105                 110         


Glu His Glu Ile Pro Val Glu Tyr Leu Glu Phe Ile Ser Lys Ala Ile 
        115                 120                 125             


Ile Gln Val Leu Gln Ser Lys His Pro Gly Lys Phe Gly Ala Lys Ala 
    130                 135                 140                 


Gln Gly Ala Met Asn Lys Ala Leu Lys Leu Phe Arg Lys Asp Met Ala 
145                 150                 155                 160 


Ser Asn Tyr Glu Glu Leu 
                165     


<210>  4
<211>  171
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Charge-modified myoglobin

<400>  4

Met His His His His His His Gly Gly Gly Gly Ser Glu Asn Leu Tyr 
1               5                   10                  15      


Phe Gln Gly Leu Ser Asp Gly Glu Trp Gln Leu Val Leu Asn Val Trp 
            20                  25                  30          


Gly Lys Val Glu Ala Asp Ile Pro Gly His Gly Gln Glu Val Leu Ile 
        35                  40                  45              


Arg Leu Phe Lys Gly His Pro Glu Thr Leu Lys Lys Phe Asp Arg Phe 
    50                  55                  60                  


Lys Lys Leu Lys Ser Glu Asp Lys Met Lys Lys Ser Glu Asp Leu Lys 
65                  70                  75                  80  


Lys His Gly Ala Thr Val Leu Lys Arg Leu Gly Gly Ile Leu Lys Lys 
                85                  90                  95      


Lys Gly Arg His Glu Ala Lys Ile Lys Pro Leu Ala Gln Arg His Ala 
            100                 105                 110         


Lys Lys His Lys Ile Pro Val Lys Tyr Leu Glu Phe Arg Ser Glu Ala 
        115                 120                 125             


Ile Ile Arg Val Leu Arg Ser Lys His Pro Gly Asp Phe Gly Ala Asp 
    130                 135                 140                 


Ala Gln Gly Ala Met Asn Lys Ala Leu Glu Leu Phe Arg Lys Asp Met 
145                 150                 155                 160 


Ala Ser Asn Tyr Lys Glu Leu Gly Phe Gln Gly 
                165                 170     


<210>  5
<211>  171
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Charge-modified myoglobin

<400>  5

Met His His His His His His Gly Gly Gly Gly Ser Glu Asn Leu Tyr 
1               5                   10                  15      


Phe Gln Gly Leu Ser Lys Gly Glu Trp Lys Leu Val Leu Asn Val Trp 
            20                  25                  30          


Gly Lys Val Glu Ala Asp Ile Pro Gly His Gly Gln Glu Val Leu Ile 
        35                  40                  45              


Arg Leu Phe Lys Gly His Pro Lys Thr Leu Lys Lys Phe Lys Arg Phe 
    50                  55                  60                  


Lys His Leu Lys Ser Glu Lys Lys Met Lys Ala Ser Lys Asp Leu Lys 
65                  70                  75                  80  


Lys His Gly Ala Thr Val Leu Thr Ala Leu Gly Gly Ile Leu Lys Lys 
                85                  90                  95      


Lys Gly His His Lys Ala Glu Ile Lys Pro Leu Ala Gln Ser His Ala 
            100                 105                 110         


Thr Lys His Lys Ile Pro Val Lys Tyr Leu Glu Phe Ile Ser Glu Ala 
        115                 120                 125             


Ile Ile Gln Val Leu Gln Ser Lys His Pro Gly Lys Phe Gly Ala Lys 
    130                 135                 140                 


Ala Gln Gly Ala Met Asn Lys Ala Leu Glu Leu Phe Arg Lys Asp Met 
145                 150                 155                 160 


Ala Ser Asn Tyr Lys Lys Leu Gly Phe Gln Gly 
                165                 170     


<210>  6
<211>  171
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Charge-modified myoglobin

<400>  6

Met His His His His His His Gly Gly Gly Gly Ser Glu Asn Leu Tyr 
1               5                   10                  15      


Phe Gln Gly Leu Ser Asp Gly Glu Trp Arg Leu Val Leu Lys Val Trp 
            20                  25                  30          


Gly Lys Val Glu Arg Asp Ile Pro Gly His Gly Gln Glu Val Leu Ile 
        35                  40                  45              


Arg Leu Phe Lys Gly His Pro Glu Thr Leu Lys Lys Phe Asp Arg Phe 
    50                  55                  60                  


Lys His Leu Lys Ser Glu Arg Glu Met Lys Ala Ser Lys Asp Leu Lys 
65                  70                  75                  80  


Lys His Gly Ala Thr Val Leu Thr Ala Leu Gly Gly Ile Leu Lys Lys 
                85                  90                  95      


Lys Gly His His Glu Ala Glu Ile Lys Pro Leu Ala Lys Ser His Ala 
            100                 105                 110         


Thr Lys His Lys Ile Pro Val Lys Tyr Leu Lys Phe Ile Ser Lys Ala 
        115                 120                 125             


Ile Ile Gln Val Leu Gln Ser Lys His Pro Gly Asp Phe Gly Ala Arg 
    130                 135                 140                 


Ala Gln Gly Ala Met Asn Lys Ala Leu Glu Leu Phe Arg Lys Asp Met 
145                 150                 155                 160 


Ala Arg Asn Tyr Lys Lys Leu Gly Phe Gln Gly 
                165                 170     


<210>  7
<211>  169
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Charge-modified myoglobin

<400>  7

Met His His His His His His Gly Gly Gly Gly Ser Glu Asn Leu Tyr 
1               5                   10                  15      


Gly Leu Ser Asp Gly Glu Trp Arg Leu Val Leu Lys Val Trp Gly Lys 
            20                  25                  30          


Val Glu Arg Asp Ile Pro Gly His Gly Gln Glu Val Leu Ile Arg Leu 
        35                  40                  45              


Phe Lys Gly His Pro Glu Thr Leu Lys Lys Phe Asp Arg Phe Lys His 
    50                  55                  60                  


Leu Lys Ser Arg Asp Glu Met Lys Ala Ser Glu Lys Leu Lys Lys His 
65                  70                  75                  80  


Gly Ala Thr Val Leu Thr Ala Leu Gly Gly Ile Leu Lys Lys Lys Gly 
                85                  90                  95      


His His Glu Ala Glu Ile Lys Pro Leu Ala Lys Ser His Ala Thr Lys 
            100                 105                 110         


His Lys Ile Pro Val Lys Tyr Leu Lys Phe Ile Ser Lys Ala Ile Ile 
        115                 120                 125             


Gln Val Leu Gln Ser Lys His Pro Gly Asp Phe Gly Ala Asp Ala Gln 
    130                 135                 140                 


Gly Ala Met Asn Lys Ala Leu Glu Leu Phe Arg Lys Asp Met Ala Ser 
145                 150                 155                 160 


Lys Tyr Lys Lys Leu Gly Phe Gln Gly 
                165                 


<210>  8
<211>  163
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Charge-modified myoglobin

<400>  8

Met His His His His His His Gly Ser Gly Gly Leu Ser Asp Gly Glu 
1               5                   10                  15      


Trp Gln Leu Val Leu Lys Val Trp Gly Lys Val Glu Ala Asp Ile Pro 
            20                  25                  30          


Gly His Gly Gln Glu Val Leu Ile Arg Leu Phe Lys Gly His Pro Glu 
        35                  40                  45              


Thr Leu Glu Lys Phe Asp Arg Phe Lys His Leu Lys Ser Glu Asp Glu 
    50                  55                  60                  


Met Lys Ala Ser Glu Asp Leu Lys Lys His Gly Ala Thr Val Leu Thr 
65                  70                  75                  80  


Ala Leu Gly Lys Ile Leu Lys Lys Lys Gly His His Glu Ala Glu Ile 
                85                  90                  95      


Lys Pro Leu Ala Gln Ser His Ala Thr Lys His Lys Ile Pro Val Lys 
            100                 105                 110         


Tyr Leu Lys Phe Ile Ser Glu Ala Ile Ile Lys Val Leu Gln Ser Lys 
        115                 120                 125             


His Pro Gly Asp Phe Gly Ala Asp Ala Gln Gly Ala Met Lys Lys Ala 
    130                 135                 140                 


Leu Glu Leu Phe Arg Lys Asp Met Ala Ser Lys Tyr Lys Glu Leu Gly 
145                 150                 155                 160 


Phe Gln Gly 
            


<210>  9
<211>  163
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Charge-modified myoglobin

<400>  9

Met His His His His His His Gly Ser Gly Gly Leu Ser Asp Gly Glu 
1               5                   10                  15      


Trp Gln Leu Val Leu Lys Val Trp Gly Lys Val Glu Ala Asp Ile Pro 
            20                  25                  30          


Gly His Gly Gln Glu Val Leu Ile Arg Leu Phe Lys Gly His Pro Glu 
        35                  40                  45              


Thr Leu Lys Lys Phe Asp Arg Phe Lys His Leu Lys Ser Glu Asp Glu 
    50                  55                  60                  


Met Lys Ala Ser Glu Asp Leu Lys Lys His Gly Ala Thr Val Leu Lys 
65                  70                  75                  80  


Lys Leu Gly Lys Ile Leu Lys Lys Lys Gly Lys His Glu Ala Glu Ile 
                85                  90                  95      


Lys Pro Leu Ala Gln Ser His Ala Thr Lys His Lys Ile Pro Val Lys 
            100                 105                 110         


Tyr Leu Lys Phe Ile Ser Glu Ala Ile Ile Lys Val Leu Gln Ser Lys 
        115                 120                 125             


His Pro Gly Asp Phe Gly Ala Asp Ala Gln Gly Ala Met Lys Lys Ala 
    130                 135                 140                 


Leu Lys Leu Phe Arg Lys Asp Met Ala Ser Lys Tyr Lys Glu Leu Gly 
145                 150                 155                 160 


Phe Gln Gly 
            


<210>  10
<211>  163
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Charge-modified myoglobin

<400>  10

Met His His His His His His Gly Ser Gly Gly Leu Ser Asp Gly Glu 
1               5                   10                  15      


Trp Gln Leu Val Leu Lys Val Trp Gly Lys Val Glu Ala Asp Ile Pro 
            20                  25                  30          


Gly His Gly Gln Glu Val Leu Ile Arg Leu Phe Lys Gly His Pro Glu 
        35                  40                  45              


Thr Leu Glu Lys Phe Asp Arg Phe Lys His Leu Lys Ser Glu Asp Glu 
    50                  55                  60                  


Met Lys Arg Ser Glu Asp Leu Lys Lys His Gly Ala Thr Val Leu Lys 
65                  70                  75                  80  


Ala Leu Gly Lys Ile Leu Lys Lys Lys Gly His His Glu Ala Glu Ile 
                85                  90                  95      


Lys Pro Leu Ala Gln Ser His Ala Thr Lys His Lys Ile Pro Val Lys 
            100                 105                 110         


Tyr Leu Lys Phe Ile Ser Glu Ala Ile Ile Lys Val Leu Gln Ser Lys 
        115                 120                 125             


His Pro Gly Asp Phe Gly Ala Asp Ala Gln Gly Ala Met Lys Lys Ala 
    130                 135                 140                 


Leu Glu Leu Phe Arg Lys Asp Met Ala Ser Lys Tyr Lys Glu Leu Gly 
145                 150                 155                 160 


Phe Gln Gly 
            


<210>  11
<211>  163
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Charge-modified myoglobin

<400>  11

Met His His His His His His Gly Ser Gly Gly Leu Ser Asp Gly Glu 
1               5                   10                  15      


Trp Gln Leu Val Leu Lys Val Trp Gly Lys Val Glu Ala Asp Ile Pro 
            20                  25                  30          


Gly His Gly Gln Glu Val Leu Ile Arg Leu Phe Lys Gly His Pro Glu 
        35                  40                  45              


Thr Leu Glu Lys Phe Asp Arg Phe Lys Lys Leu Lys Ser Glu Asp Glu 
    50                  55                  60                  


Met Lys Arg Ser Glu Asp Leu Lys Lys His Gly Ala Thr Val Leu Lys 
65                  70                  75                  80  


Lys Leu Gly Lys Ile Leu Lys Lys Lys Gly Lys His Glu Ala Glu Ile 
                85                  90                  95      


Lys Pro Leu Ala Gln Ser His Ala Thr Lys His Lys Ile Pro Val Lys 
            100                 105                 110         


Tyr Leu Lys Phe Ile Ser Glu Ala Ile Ile Lys Val Leu Gln Ser Lys 
        115                 120                 125             


His Pro Gly Asp Phe Gly Ala Asp Ala Gln Gly Ala Met Lys Lys Ala 
    130                 135                 140                 


Leu Lys Leu Phe Arg Lys Asp Met Ala Ser Lys Tyr Lys Glu Leu Gly 
145                 150                 155                 160 


Phe Gln Gly 
            


<210>  12
<211>  163
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Charge-modified myoglobin

<400>  12

Met His His His His His His Gly Ser Gly Gly Leu Arg Asp Gly Glu 
1               5                   10                  15      


Trp Gln Leu Val Leu Lys Val Trp Gly Lys Val Glu Ala Asp Ile Pro 
            20                  25                  30          


Gly His Gly Gln Glu Val Leu Ile Arg Leu Phe Lys Gly His Pro Glu 
        35                  40                  45              


Thr Leu Glu Lys Phe Asp Arg Phe Lys Lys Leu Lys Ser Glu Asp Glu 
    50                  55                  60                  


Met Lys Arg Ser Glu Asp Leu Lys Lys His Gly Ala Thr Val Leu Lys 
65                  70                  75                  80  


Lys Leu Gly Lys Ile Leu Lys Lys Lys Gly Lys His Glu Ala Glu Ile 
                85                  90                  95      


Lys Pro Leu Ala Gln Ser His Ala Thr Lys His Lys Ile Pro Val Lys 
            100                 105                 110         


Tyr Leu Lys Phe Ile Ser Glu Ala Ile Ile Lys Val Leu Gln Ser Lys 
        115                 120                 125             


His Pro Gly Asp Phe Gly Ala Asp Ala Gln Gly Ala Met Lys Lys Ala 
    130                 135                 140                 


Leu Lys Leu Phe Arg Lys Asp Met Ala Ser Lys Tyr Lys Glu Leu Gly 
145                 150                 155                 160 


Phe Gln Gly 
            


<210>  13
<211>  695
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Charge-modified myoglobin

<400>  13

Met His His His His His His Lys Ile Glu Glu Gly Lys Leu Val Ile 
1               5                   10                  15      


Trp Ile Asn Gly Asp Lys Gly Tyr Asn Gly Leu Ala Glu Val Gly Lys 
            20                  25                  30          


Lys Phe Glu Lys Asp Thr Gly Ile Lys Val Thr Val Glu His Pro Asp 
        35                  40                  45              


Lys Leu Glu Glu Lys Phe Pro Gln Val Ala Ala Thr Gly Asp Gly Pro 
    50                  55                  60                  


Asp Ile Ile Phe Trp Ala His Asp Arg Phe Gly Gly Tyr Ala Gln Ser 
65                  70                  75                  80  


Gly Leu Leu Ala Glu Ile Thr Pro Asp Lys Ala Phe Gln Asp Lys Leu 
                85                  90                  95      


Tyr Pro Phe Thr Trp Asp Ala Val Arg Tyr Asn Gly Lys Leu Ile Ala 
            100                 105                 110         


Tyr Pro Ile Ala Val Glu Ala Leu Ser Leu Ile Tyr Asn Lys Asp Leu 
        115                 120                 125             


Leu Pro Asn Pro Pro Lys Thr Trp Glu Glu Ile Pro Ala Leu Asp Lys 
    130                 135                 140                 


Glu Leu Lys Ala Lys Gly Lys Ser Ala Leu Met Phe Asn Leu Gln Glu 
145                 150                 155                 160 


Pro Tyr Phe Thr Trp Pro Leu Ile Ala Ala Asp Gly Gly Tyr Ala Phe 
                165                 170                 175     


Lys Tyr Glu Asn Gly Lys Tyr Asp Ile Lys Asp Val Gly Val Asp Asn 
            180                 185                 190         


Ala Gly Ala Lys Ala Gly Leu Thr Phe Leu Val Asp Leu Ile Lys Asn 
        195                 200                 205             


Lys His Met Asn Ala Asp Thr Asp Tyr Ser Ile Ala Glu Ala Ala Phe 
    210                 215                 220                 


Asn Lys Gly Glu Thr Ala Met Thr Ile Asn Gly Pro Trp Ala Trp Ser 
225                 230                 235                 240 


Asn Ile Asp Thr Ser Lys Val Asn Tyr Gly Val Thr Val Leu Pro Thr 
                245                 250                 255     


Phe Lys Gly Gln Pro Ser Lys Pro Phe Val Gly Val Leu Ser Ala Gly 
            260                 265                 270         


Ile Asn Ala Ala Ser Pro Asn Lys Glu Leu Ala Lys Glu Phe Leu Glu 
        275                 280                 285             


Asn Tyr Leu Leu Thr Asp Glu Gly Leu Glu Ala Val Asn Lys Asp Lys 
    290                 295                 300                 


Pro Leu Gly Ala Val Ala Leu Lys Ser Tyr Glu Glu Glu Leu Val Lys 
305                 310                 315                 320 


Asp Pro Arg Ile Ala Ala Thr Met Glu Asn Ala Gln Lys Gly Glu Ile 
                325                 330                 335     


Met Pro Asn Ile Pro Gln Met Ser Ala Phe Trp Tyr Ala Val Arg Thr 
            340                 345                 350         


Ala Val Ile Asn Ala Ala Ser Gly Arg Gln Thr Val Asp Glu Ala Leu 
        355                 360                 365             


Lys Asp Ala Gln Thr Asn Ser Ser Ser Asn Asn Asn Asn Asn Asn Asn 
    370                 375                 380                 


Asn Asn Asn Leu Gly Glu Asn Leu Tyr Phe Gln Gly Ser Gly Trp Asn 
385                 390                 395                 400 


Pro Pro Thr Phe Ser Pro Ala Leu Leu Val Val Thr Glu Gly Asp Asn 
                405                 410                 415     


Ala Thr Phe Thr Cys Ser Phe Ser Asn Thr Ser Glu Ser Phe Val Leu 
            420                 425                 430         


Asn Trp Tyr Arg Met Ser Pro Ser Asn Gln Thr Val Lys Leu Ala Ala 
        435                 440                 445             


Phe Pro Glu Asp Arg Ser Gln Pro Gly Gln Asp Ser Arg Phe Arg Val 
    450                 455                 460                 


Thr Gln Leu Pro Asn Gly Arg Asp Phe His Met Ser Val Val Arg Ala 
465                 470                 475                 480 


Arg Arg Asn Asp Ser Gly Thr Tyr Leu Cys Ser Ala Ile Ser Leu Ala 
                485                 490                 495     


Pro Lys Ala Gln Ile Lys Glu Ser Leu Arg Ala Glu Leu Arg Val Thr 
            500                 505                 510         


Glu Arg Arg Ala Glu Val Pro Thr Ala His Pro Ser Pro Ser Pro Gly 
        515                 520                 525             


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Leu 
    530                 535                 540                 


Ser Asp Gly Glu Trp Gln Leu Val Leu Lys Val Trp Gly Lys Val Glu 
545                 550                 555                 560 


Ala Asp Ile Pro Gly His Gly Gln Glu Val Leu Ile Arg Leu Phe Lys 
                565                 570                 575     


Gly His Pro Glu Thr Leu Lys Lys Phe Asp Arg Phe Lys His Leu Lys 
            580                 585                 590         


Ser Glu Asp Glu Met Lys Ala Ser Glu Asp Leu Lys Lys His Gly Ala 
        595                 600                 605             


Thr Val Leu Lys Lys Leu Gly Lys Ile Leu Lys Lys Lys Gly Lys His 
    610                 615                 620                 


Glu Ala Glu Ile Lys Pro Leu Ala Gln Ser His Ala Thr Lys His Lys 
625                 630                 635                 640 


Ile Pro Val Lys Tyr Leu Lys Phe Ile Ser Glu Ala Ile Ile Lys Val 
                645                 650                 655     


Leu Gln Ser Lys His Pro Gly Asp Phe Gly Ala Asp Ala Gln Gly Ala 
            660                 665                 670         


Met Lys Lys Ala Leu Lys Leu Phe Arg Lys Asp Met Ala Ser Lys Tyr 
        675                 680                 685             


Lys Glu Leu Gly Phe Gln Gly 
    690                 695 


<210>  14
<211>  689
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Charge-modified myoglobin

<400>  14

Met Lys Ile Glu Glu Gly Lys Leu Val Ile Trp Ile Asn Gly Asp Lys 
1               5                   10                  15      


Gly Tyr Asn Gly Leu Ala Glu Val Gly Lys Lys Phe Glu Lys Asp Thr 
            20                  25                  30          


Gly Ile Lys Val Thr Val Glu His Pro Asp Lys Leu Glu Glu Lys Phe 
        35                  40                  45              


Pro Gln Val Ala Ala Thr Gly Asp Gly Pro Asp Ile Ile Phe Trp Ala 
    50                  55                  60                  


His Asp Arg Phe Gly Gly Tyr Ala Gln Ser Gly Leu Leu Ala Glu Ile 
65                  70                  75                  80  


Thr Pro Asp Lys Ala Phe Gln Asp Lys Leu Tyr Pro Phe Thr Trp Asp 
                85                  90                  95      


Ala Val Arg Tyr Asn Gly Lys Leu Ile Ala Tyr Pro Ile Ala Val Glu 
            100                 105                 110         


Ala Leu Ser Leu Ile Tyr Asn Lys Asp Leu Leu Pro Asn Pro Pro Lys 
        115                 120                 125             


Thr Trp Glu Glu Ile Pro Ala Leu Asp Lys Glu Leu Lys Ala Lys Gly 
    130                 135                 140                 


Lys Ser Ala Leu Met Phe Asn Leu Gln Glu Pro Tyr Phe Thr Trp Pro 
145                 150                 155                 160 


Leu Ile Ala Ala Asp Gly Gly Tyr Ala Phe Lys Tyr Glu Asn Gly Lys 
                165                 170                 175     


Tyr Asp Ile Lys Asp Val Gly Val Asp Asn Ala Gly Ala Lys Ala Gly 
            180                 185                 190         


Leu Thr Phe Leu Val Asp Leu Ile Lys Asn Lys His Met Asn Ala Asp 
        195                 200                 205             


Thr Asp Tyr Ser Ile Ala Glu Ala Ala Phe Asn Lys Gly Glu Thr Ala 
    210                 215                 220                 


Met Thr Ile Asn Gly Pro Trp Ala Trp Ser Asn Ile Asp Thr Ser Lys 
225                 230                 235                 240 


Val Asn Tyr Gly Val Thr Val Leu Pro Thr Phe Lys Gly Gln Pro Ser 
                245                 250                 255     


Lys Pro Phe Val Gly Val Leu Ser Ala Gly Ile Asn Ala Ala Ser Pro 
            260                 265                 270         


Asn Lys Glu Leu Ala Lys Glu Phe Leu Glu Asn Tyr Leu Leu Thr Asp 
        275                 280                 285             


Glu Gly Leu Glu Ala Val Asn Lys Asp Lys Pro Leu Gly Ala Val Ala 
    290                 295                 300                 


Leu Lys Ser Tyr Glu Glu Glu Leu Val Lys Asp Pro Arg Ile Ala Ala 
305                 310                 315                 320 


Thr Met Glu Asn Ala Gln Lys Gly Glu Ile Met Pro Asn Ile Pro Gln 
                325                 330                 335     


Met Ser Ala Phe Trp Tyr Ala Val Arg Thr Ala Val Ile Asn Ala Ala 
            340                 345                 350         


Ser Gly Arg Gln Thr Val Asp Glu Ala Leu Lys Asp Ala Gln Thr Asn 
        355                 360                 365             


Ser Ser Ser Asn Asn Asn Asn Asn Asn Asn Asn Asn Asn Leu Gly Glu 
    370                 375                 380                 


Asn Leu Tyr Phe Gln Gly Ser Gly Trp Asn Pro Pro Thr Phe Ser Pro 
385                 390                 395                 400 


Ala Leu Leu Val Val Thr Glu Gly Asp Asn Ala Thr Phe Thr Cys Ser 
                405                 410                 415     


Phe Ser Asn Thr Ser Glu Ser Phe Val Leu Asn Trp Tyr Arg Met Ser 
            420                 425                 430         


Pro Ser Asn Gln Thr Val Lys Leu Ala Ala Phe Pro Glu Asp Arg Ser 
        435                 440                 445             


Gln Pro Gly Gln Asp Ser Arg Phe Arg Val Thr Gln Leu Pro Asn Gly 
    450                 455                 460                 


Arg Asp Phe His Met Ser Val Val Arg Ala Arg Arg Asn Asp Ser Gly 
465                 470                 475                 480 


Thr Tyr Leu Cys Ser Ala Ile Ser Leu Ala Pro Lys Ala Gln Ile Lys 
                485                 490                 495     


Glu Ser Leu Arg Ala Glu Leu Arg Val Thr Glu Arg Arg Ala Glu Val 
            500                 505                 510         


Pro Thr Ala His Pro Ser Pro Ser Pro Gly Gly Gly Gly Ser Gly Gly 
        515                 520                 525             


Gly Gly Ser Gly Gly Gly Gly Ser Gly Leu Ser Asp Gly Glu Trp Gln 
    530                 535                 540                 


Leu Val Leu Lys Val Trp Gly Lys Val Glu Ala Asp Ile Pro Gly His 
545                 550                 555                 560 


Gly Gln Glu Val Leu Ile Arg Leu Phe Lys Gly His Pro Glu Thr Leu 
                565                 570                 575     


Lys Lys Phe Asp Arg Phe Lys His Leu Lys Ser Glu Asp Glu Met Lys 
            580                 585                 590         


Ala Ser Glu Asp Leu Lys Lys His Gly Ala Thr Val Leu Lys Lys Leu 
        595                 600                 605             


Gly Lys Ile Leu Lys Lys Lys Gly Lys His Glu Ala Glu Ile Lys Pro 
    610                 615                 620                 


Leu Ala Gln Ser His Ala Thr Lys His Lys Ile Pro Val Lys Tyr Leu 
625                 630                 635                 640 


Lys Phe Ile Ser Glu Ala Ile Ile Lys Val Leu Gln Ser Lys His Pro 
                645                 650                 655     


Gly Asp Phe Gly Ala Asp Ala Gln Gly Ala Met Lys Lys Ala Leu Lys 
            660                 665                 670         


Leu Phe Arg Lys Asp Met Ala Ser Lys Tyr Lys Glu Leu Gly Phe Gln 
        675                 680                 685             


Gly 
    


<210>  15
<211>  239
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Charge-modified GFP

<400>  15

Met Ala Ser Lys Gly Glu Arg Leu Phe Arg Gly Lys Val Pro Ile Leu 
1               5                   10                  15      


Val Glu Leu Lys Gly Asp Val Asn Gly His Lys Phe Ser Val Arg Gly 
            20                  25                  30          


Lys Gly Lys Gly Asp Ala Thr Arg Gly Lys Leu Thr Leu Lys Phe Ile 
        35                  40                  45              


Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr 
    50                  55                  60                  


Leu Thr Tyr Gly Val Gln Cys Phe Ser Arg Tyr Pro Lys His Met Lys 
65                  70                  75                  80  


Arg His Asp Phe Phe Lys Ser Ala Met Pro Lys Gly Tyr Val Gln Glu 
                85                  90                  95      


Arg Thr Ile Ser Phe Lys Lys Asp Gly Lys Tyr Lys Thr Arg Ala Glu 
            100                 105                 110         


Val Lys Phe Glu Gly Arg Thr Leu Val Asn Arg Ile Lys Leu Lys Gly 
        115                 120                 125             


Arg Asp Phe Lys Glu Lys Gly Asn Ile Leu Gly His Lys Leu Arg Tyr 
    130                 135                 140                 


Asn Phe Asn Ser His Lys Val Tyr Ile Thr Ala Asp Lys Arg Lys Asn 
145                 150                 155                 160 


Gly Ile Lys Ala Lys Phe Lys Ile Arg His Asn Val Lys Asp Gly Ser 
                165                 170                 175     


Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Arg Gly 
            180                 185                 190         


Pro Val Leu Leu Pro Arg Asn His Tyr Leu Ser Thr Arg Ser Lys Leu 
        195                 200                 205             


Ser Lys Asp Pro Lys Glu Lys Arg Asp His Met Val Leu Leu Glu Phe 
    210                 215                 220                 


Val Thr Ala Ala Gly Ile Lys His Gly Arg Asp Glu Arg Tyr Lys 
225                 230                 235                 


