                         SEQUENCE LISTING

<110>  GlaxoSmithKline
 
<120>  HEPATITIS B IMMUNISATION REGIMEN AND COMPOSITIONS

<130>  PR66724 WO

<150>  US 62/814261
<151>  2019-03-05

<160>  28    

<170>  PatentIn version 3.5

<210>  1
<211>  226
<212>  PRT
<213>  Hepatitis B virus

<400>  1

Met Glu Asn Ile Thr Ser Gly Phe Leu Gly Pro Leu Leu Val Leu Gln 
1               5                   10                  15      


Ala Gly Phe Phe Leu Leu Thr Arg Ile Leu Thr Ile Pro Gln Ser Leu 
            20                  25                  30          


Asp Ser Trp Trp Thr Ser Leu Asn Phe Leu Gly Gly Ser Pro Val Cys 
        35                  40                  45              


Leu Gly Gln Asn Ser Gln Ser Pro Thr Ser Asn His Ser Pro Thr Ser 
    50                  55                  60                  


Cys Pro Pro Ile Cys Pro Gly Tyr Arg Trp Met Cys Leu Arg Arg Phe 
65                  70                  75                  80  


Ile Ile Phe Leu Phe Ile Leu Leu Leu Cys Leu Ile Phe Leu Leu Val 
                85                  90                  95      


Leu Leu Asp Tyr Gln Gly Met Leu Pro Val Cys Pro Leu Ile Pro Gly 
            100                 105                 110         


Ser Thr Thr Thr Asn Thr Gly Pro Cys Lys Thr Cys Thr Thr Pro Ala 
        115                 120                 125             


Gln Gly Asn Ser Met Phe Pro Ser Cys Cys Cys Thr Lys Pro Thr Asp 
    130                 135                 140                 


Gly Asn Cys Thr Cys Ile Pro Ile Pro Ser Ser Trp Ala Phe Ala Lys 
145                 150                 155                 160 


Tyr Leu Trp Glu Trp Ala Ser Val Arg Phe Ser Trp Leu Ser Leu Leu 
                165                 170                 175     


Val Pro Phe Val Gln Trp Phe Val Gly Leu Ser Pro Thr Val Trp Leu 
            180                 185                 190         


Ser Ala Ile Trp Met Met Trp Tyr Trp Gly Pro Ser Leu Tyr Ser Ile 
        195                 200                 205             


Val Ser Pro Phe Ile Pro Leu Leu Pro Ile Phe Phe Cys Leu Trp Val 
    210                 215                 220                 


Tyr Ile 
225     


<210>  2
<211>  149
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of HBc truncate

<400>  2

Met Asp Ile Asp Pro Tyr Lys Glu Phe Gly Ala Thr Val Glu Leu Leu 
1               5                   10                  15      


Ser Phe Leu Pro Ser Asp Phe Phe Pro Ser Val Arg Asp Leu Leu Asp 
            20                  25                  30          


Thr Ala Ser Ala Leu Tyr Arg Glu Ala Leu Glu Ser Pro Glu His Cys 
        35                  40                  45              


Ser Pro His His Thr Ala Leu Arg Gln Ala Ile Leu Cys Trp Gly Glu 
    50                  55                  60                  


Leu Met Thr Leu Ala Thr Trp Val Gly Asn Asn Leu Glu Asp Pro Ala 
65                  70                  75                  80  


Ser Arg Asp Leu Val Val Asn Tyr Val Asn Thr Asn Met Gly Leu Lys 
                85                  90                  95      


Ile Arg Gln Leu Leu Trp Phe His Ile Ser Cys Leu Thr Phe Gly Arg 
            100                 105                 110         


Glu Thr Val Leu Glu Tyr Leu Val Ser Phe Gly Val Trp Ile Arg Thr 
        115                 120                 125             


Pro Pro Ala Tyr Arg Pro Pro Asn Ala Pro Ile Leu Ser Thr Leu Pro 
    130                 135                 140                 


Glu Thr Thr Val Val 
145                 


<210>  3
<211>  24
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of spacer incorporating 2A cleaving region of
       the foot and mouth disease virus

<400>  3

Ala Pro Val Lys Gln Thr Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly 
1               5                   10                  15      


Asp Val Glu Ser Asn Pro Gly Pro 
            20                  


<210>  4
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence encoding spacer incorporating 2A cleavage 
       region of the foot and mouth disease virus

<400>  4
gcccctgtga agcagaccct gaacttcgac ctgctgaagc tggccggcga cgtggagagc       60

aatcccggcc ct                                                           72


<210>  5
<211>  435
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of HBc-2A-HBs

<400>  5

Met Asp Ile Asp Pro Tyr Lys Glu Phe Gly Ala Thr Val Glu Leu Leu 
1               5                   10                  15      


Ser Phe Leu Pro Ser Asp Phe Phe Pro Ser Val Arg Asp Leu Leu Asp 
            20                  25                  30          


Thr Ala Ser Ala Leu Tyr Arg Glu Ala Leu Glu Ser Pro Glu His Cys 
        35                  40                  45              


Ser Pro His His Thr Ala Leu Arg Gln Ala Ile Leu Cys Trp Gly Glu 
    50                  55                  60                  


Leu Met Thr Leu Ala Thr Trp Val Gly Asn Asn Leu Glu Asp Pro Ala 
65                  70                  75                  80  


Ser Arg Asp Leu Val Val Asn Tyr Val Asn Thr Asn Met Gly Leu Lys 
                85                  90                  95      


Ile Arg Gln Leu Leu Trp Phe His Ile Ser Cys Leu Thr Phe Gly Arg 
            100                 105                 110         


Glu Thr Val Leu Glu Tyr Leu Val Ser Phe Gly Val Trp Ile Arg Thr 
        115                 120                 125             


Pro Pro Ala Tyr Arg Pro Pro Asn Ala Pro Ile Leu Ser Thr Leu Pro 
    130                 135                 140                 


Glu Thr Thr Val Val Arg Arg Arg Asp Arg Gly Arg Ser Pro Arg Arg 
145                 150                 155                 160 


Arg Thr Pro Ser Pro Arg Arg Arg Arg Ser Gln Ser Pro Arg Arg Arg 
                165                 170                 175     


Arg Ser Gln Ser Arg Glu Ser Gln Cys Ala Pro Val Lys Gln Thr Leu 
            180                 185                 190         


Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val Glu Ser Asn Pro Gly 
        195                 200                 205             


Pro Met Glu Asn Ile Thr Ser Gly Phe Leu Gly Pro Leu Leu Val Leu 
    210                 215                 220                 


Gln Ala Gly Phe Phe Leu Leu Thr Arg Ile Leu Thr Ile Pro Gln Ser 
225                 230                 235                 240 


Leu Asp Ser Trp Trp Thr Ser Leu Asn Phe Leu Gly Gly Ser Pro Val 
                245                 250                 255     


Cys Leu Gly Gln Asn Ser Gln Ser Pro Thr Ser Asn His Ser Pro Thr 
            260                 265                 270         


Ser Cys Pro Pro Ile Cys Pro Gly Tyr Arg Trp Met Cys Leu Arg Arg 
        275                 280                 285             


Phe Ile Ile Phe Leu Phe Ile Leu Leu Leu Cys Leu Ile Phe Leu Leu 
    290                 295                 300                 


Val Leu Leu Asp Tyr Gln Gly Met Leu Pro Val Cys Pro Leu Ile Pro 
305                 310                 315                 320 


Gly Ser Thr Thr Thr Asn Thr Gly Pro Cys Lys Thr Cys Thr Thr Pro 
                325                 330                 335     


Ala Gln Gly Asn Ser Met Phe Pro Ser Cys Cys Cys Thr Lys Pro Thr 
            340                 345                 350         


Asp Gly Asn Cys Thr Cys Ile Pro Ile Pro Ser Ser Trp Ala Phe Ala 
        355                 360                 365             


Lys Tyr Leu Trp Glu Trp Ala Ser Val Arg Phe Ser Trp Leu Ser Leu 
    370                 375                 380                 


Leu Val Pro Phe Val Gln Trp Phe Val Gly Leu Ser Pro Thr Val Trp 
385                 390                 395                 400 


Leu Ser Ala Ile Trp Met Met Trp Tyr Trp Gly Pro Ser Leu Tyr Ser 
                405                 410                 415     


Ile Val Ser Pro Phe Ile Pro Leu Leu Pro Ile Phe Phe Cys Leu Trp 
            420                 425                 430         


Val Tyr Ile 
        435 


<210>  6
<211>  1305
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence encoding HBc-2A-HBs

<400>  6
atggacatcg atccctacaa ggaatttggc gccaccgtgg agctgctgag cttcctgccc       60

agcgacttct tccccagcgt gagggacctc ctggacaccg ccagcgccct gtacagggag      120

gccctggaat ctcccgagca ctgcagccca caccacaccg cactgaggca ggccatcctg      180

tgctggggag agctgatgac cctcgccacc tgggtgggca acaacctgga ggaccccgcc      240

agcagggacc tggtggtgaa ctacgtcaac accaacatgg gcctgaagat caggcagctg      300

ctgtggttcc acatcagctg cctgaccttc ggcagggaga ccgtgctgga gtacctggtg      360

agcttcggcg tgtggatcag gacacctccc gcctacagac cccccaacgc ccccatcctg      420

agcaccctgc ccgagaccac agtggtgagg aggagggaca ggggcaggtc acccaggagg      480

aggactccaa gccccaggag gaggaggagc cagagcccca ggagaaggag gagccagagc      540

agggagagcc agtgcgcccc tgtgaagcag accctgaact tcgacctgct gaagctggcc      600

ggcgacgtgg agagcaatcc cggccctatg gagaacatca ccagcggctt cctgggcccc      660

ctgctggtgc tgcaggcagg cttcttcctg ctgaccagga tcctgaccat cccccagagc      720

ctggacagct ggtggaccag cctgaacttc ctcggcggga gccccgtgtg cctgggccag      780

aacagccagt ctcccaccag caatcacagc cccaccagct gccccccaat ctgtcctggc      840

taccggtgga tgtgcctgag gaggttcatc atcttcctgt tcatcctgct cctgtgcctg      900

atcttcctgc tggtgctgct ggactaccag ggaatgctgc cagtgtgtcc cctgatcccc      960

ggctcaacca ccactaacac cggcccctgc aaaacctgca ccacccccgc tcagggcaac     1020

agcatgttcc caagctgctg ctgcaccaag cccaccgacg gcaactgcac ctgcattccc     1080

atccccagca gctgggcctt cgccaagtat ctgtgggagt gggccagcgt gaggttcagc     1140

tggctcagcc tgctggtgcc cttcgtccag tggtttgtgg gcctgagccc caccgtgtgg     1200

ctgagcgcca tctggatgat gtggtactgg ggccccagcc tgtactccat cgtgagcccc     1260

ttcatccccc tgctgcccat tttcttctgc ctgtgggtgt acatc                     1305


<210>  7
<211>  229
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of hIi

<400>  7

Met His Arg Arg Arg Ser Arg Ser Cys Arg Glu Asp Gln Lys Pro Val 
1               5                   10                  15      


Met Asp Asp Gln Arg Asp Leu Ile Ser Asn Asn Glu Gln Leu Pro Met 
            20                  25                  30          


Leu Gly Arg Arg Pro Gly Ala Pro Glu Ser Lys Cys Ser Arg Gly Ala 
        35                  40                  45              


Leu Tyr Thr Gly Phe Ser Ile Leu Val Thr Leu Leu Leu Ala Gly Gln 
    50                  55                  60                  


Ala Thr Thr Ala Tyr Phe Leu Tyr Gln Gln Gln Gly Arg Leu Asp Lys 
65                  70                  75                  80  


Leu Thr Val Thr Ser Gln Asn Leu Gln Leu Glu Asn Leu Arg Met Lys 
                85                  90                  95      


Leu Pro Lys Pro Lys Pro Val Ser Lys Met Arg Met Ala Thr Pro Leu 
            100                 105                 110         


Leu Met Gln Ala Leu Pro Met Gly Ala Leu Pro Gln Gly Pro Met Gln 
        115                 120                 125             


Asn Ala Thr Lys Tyr Gly Asn Met Thr Glu Asp His Val Met His Leu 
    130                 135                 140                 


Leu Gln Asn Ala Asp Pro Leu Lys Val Tyr Pro Pro Leu Lys Ser Phe 
145                 150                 155                 160 


Pro Glu Asn Leu Arg His Leu Lys Asn Thr Met Glu Thr Ile Asp Trp 
                165                 170                 175     


Lys Val Phe Glu Ser Trp Met His His Trp Leu Leu Phe Glu Met Ser 
            180                 185                 190         


Arg His Ser Leu Glu Gln Lys Pro Thr Asp Ala Pro Pro Lys Glu Ser 
        195                 200                 205             


Leu Glu Leu Glu Asp Pro Ser Ser Gly Gly Val Thr Lys Gln Asp Leu 
    210                 215                 220                 


Gly Pro Val Pro Met 
225                 


<210>  8
<211>  696
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence encoding hIi

<400>  8
atgcacagga ggaggagcag gagctgcagg gaggaccaga agcccgtgat ggacgaccag       60

cgcgacctga tcagcaacaa cgagcagctg ccaatgctgg gcaggaggcc cggagcaccc      120

gaaagcaagt gcagcagggg cgccctgtac accggcttca gcatcctggt gaccctcctg      180

ctggccggcc aggccaccac cgcctatttc ctgtaccagc agcagggcag gctcgataag      240

ctgaccgtga cctcccagaa cctgcagctg gagaacctga ggatgaagct gcccaagccc      300

cccaagcccg tgagcaagat gaggatggcc acccccctgc tgatgcaggc tctgcccatg      360

ggggccctgc cccagggccc catgcagaac gccaccaaat acggcaacat gaccgaggac      420

cacgtgatgc acctgctgca gaacgccgat cctctgaagg tgtacccacc cctgaaaggc      480

agcttccccg agaacctcag gcacctgaag aacaccatgg agaccatcga ctggaaggtg      540

ttcgagagct ggatgcacca ctggctgctg ttcgagatga gccggcacag cctggagcag      600

aagcccaccg acgcccctcc caaggagagc ctcgagctcg aggacccaag cagcggcctg      660

ggcgtgacca agcaggacct gggccccgtg cccatg                                696


<210>  9
<211>  628
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of hIi-HBc-2A-HBs

<400>  9

Met His Arg Arg Arg Ser Arg Ser Cys Arg Glu Asp Gln Lys Pro Val 
1               5                   10                  15      


Met Asp Asp Gln Arg Asp Leu Ile Ser Asn Asn Glu Gln Leu Pro Met 
            20                  25                  30          


Leu Gly Arg Arg Pro Gly Ala Pro Glu Ser Lys Cys Ser Arg Gly Ala 
        35                  40                  45              


Leu Tyr Thr Gly Phe Ser Ile Leu Val Thr Leu Leu Leu Ala Gly Gln 
    50                  55                  60                  


Ala Thr Thr Ala Tyr Phe Leu Tyr Gln Gln Gln Gly Arg Leu Asp Lys 
65                  70                  75                  80  


Leu Thr Val Thr Ser Gln Asn Leu Gln Leu Glu Asn Leu Arg Met Lys 
                85                  90                  95      


Leu Pro Lys Pro Lys Pro Val Ser Lys Met Arg Met Ala Thr Pro Leu 
            100                 105                 110         


Leu Met Gln Ala Leu Pro Met Gly Ala Leu Pro Gln Gly Pro Met Gln 
        115                 120                 125             


Asn Ala Thr Lys Tyr Gly Asn Met Thr Glu Asp His Val Met His Leu 
    130                 135                 140                 


Leu Gln Asn Ala Asp Pro Leu Lys Val Tyr Pro Pro Leu Lys Ser Phe 
145                 150                 155                 160 


Pro Glu Asn Leu Arg His Leu Lys Asn Thr Met Glu Thr Ile Asp Trp 
                165                 170                 175     


Lys Val Phe Glu Ser Trp Met His His Trp Leu Leu Phe Glu Met Ser 
            180                 185                 190         


Arg His Ser Leu Glu Gln Lys Pro Thr Asp Ala Pro Pro Lys Glu Ser 
        195                 200                 205             


Leu Glu Leu Glu Asp Pro Ser Ser Gly Gly Val Thr Lys Gln Asp Leu 
    210                 215                 220                 


Gly Pro Val Pro Met Met Asp Ile Asp Pro Tyr Lys Glu Phe Gly Ala 
225                 230                 235                 240 


Thr Val Glu Leu Leu Ser Phe Leu Pro Ser Asp Phe Phe Pro Ser Val 
                245                 250                 255     


Arg Asp Leu Leu Asp Thr Ala Ser Ala Leu Tyr Arg Glu Ala Leu Glu 
            260                 265                 270         


Ser Pro Glu His Cys Ser Pro His His Thr Ala Leu Arg Gln Ala Ile 
        275                 280                 285             


Leu Cys Trp Gly Glu Leu Met Thr Leu Ala Thr Trp Val Gly Asn Asn 
    290                 295                 300                 


Leu Glu Asp Pro Ala Ser Arg Asp Leu Val Val Asn Tyr Val Asn Thr 
305                 310                 315                 320 


Asn Met Gly Leu Lys Ile Arg Gln Leu Leu Trp Phe His Ile Ser Cys 
                325                 330                 335     


Leu Thr Phe Gly Arg Glu Thr Val Leu Glu Tyr Leu Val Ser Phe Gly 
            340                 345                 350         


Val Trp Ile Arg Thr Pro Pro Ala Tyr Arg Pro Pro Asn Ala Pro Ile 
        355                 360                 365             


Leu Ser Thr Leu Pro Glu Thr Thr Val Val Ala Pro Val Lys Gln Thr 
    370                 375                 380                 


Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val Glu Ser Asn Pro 
385                 390                 395                 400 


Gly Pro Met Glu Asn Ile Thr Ser Gly Phe Leu Gly Pro Leu Leu Val 
                405                 410                 415     


Leu Gln Ala Gly Phe Phe Leu Leu Thr Arg Ile Leu Thr Ile Pro Gln 
            420                 425                 430         


Ser Leu Asp Ser Trp Trp Thr Ser Leu Asn Phe Leu Gly Gly Ser Pro 
        435                 440                 445             


Val Cys Leu Gly Gln Asn Ser Gln Ser Pro Thr Ser Asn His Ser Pro 
    450                 455                 460                 


Thr Ser Cys Pro Pro Ile Cys Pro Gly Tyr Arg Trp Met Cys Leu Arg 
465                 470                 475                 480 


Arg Phe Ile Ile Phe Leu Phe Ile Leu Leu Leu Cys Leu Ile Phe Leu 
                485                 490                 495     


Leu Val Leu Leu Asp Tyr Gln Gly Met Leu Pro Val Cys Pro Leu Ile 
            500                 505                 510         


Pro Gly Ser Thr Thr Thr Asn Thr Gly Pro Cys Lys Thr Cys Thr Thr 
        515                 520                 525             


Pro Ala Gln Gly Asn Ser Met Phe Pro Ser Cys Cys Cys Thr Lys Pro 
    530                 535                 540                 


Thr Asp Gly Asn Cys Thr Cys Ile Pro Ile Pro Ser Ser Trp Ala Phe 
545                 550                 555                 560 


Ala Lys Tyr Leu Trp Glu Trp Ala Ser Val Arg Phe Ser Trp Leu Ser 
                565                 570                 575     


Leu Leu Val Pro Phe Val Gln Trp Phe Val Gly Leu Ser Pro Thr Val 
            580                 585                 590         


Trp Leu Ser Ala Ile Trp Met Met Trp Tyr Trp Gly Pro Ser Leu Tyr 
        595                 600                 605             


Ser Ile Val Ser Pro Phe Ile Pro Leu Leu Pro Ile Phe Phe Cys Leu 
    610                 615                 620                 


Trp Val Tyr Ile 
625             


<210>  10
<211>  1998
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence encoding hIi-HBc-2A-HBs

<400>  10
atgcacagga ggaggagcag gagctgcagg gaggaccaga agcccgtgat ggacgaccag       60

cgcgacctga tcagcaacaa cgagcagctg ccaatgctgg gcaggaggcc cggagcaccc      120

gaaagcaagt gcagcagggg cgccctgtac accggcttca gcatcctggt gaccctcctg      180

ctggccggcc aggccaccac cgcctatttc ctgtaccagc agcagggcag gctcgataag      240

ctgaccgtga cctcccagaa cctgcagctg gagaacctga ggatgaagct gcccaagccc      300

cccaagcccg tgagcaagat gaggatggcc acccccctgc tgatgcaggc tctgcccatg      360

ggggccctgc cccagggccc catgcagaac gccaccaaat acggcaacat gaccgaggac      420

cacgtgatgc acctgctgca gaacgccgat cctctgaagg tgtacccacc cctgaaaggc      480

agcttccccg agaacctcag gcacctgaag aacaccatgg agaccatcga ctggaaggtg      540

ttcgagagct ggatgcacca ctggctgctg ttcgagatga gccggcacag cctggagcag      600

aagcccaccg acgcccctcc caaggagagc ctcgagctcg aggacccaag cagcggcctg      660

ggcgtgacca agcaggacct gggccccgtg cccatggaca ttgaccccta caaggagttc      720

ggcgccaccg tcgaactgct gagcttcctc cccagcgact tcttcccctc cgtgagggat      780

ctgctggaca cagctagcgc cctgtacagg gaggccctgg agagccccga gcactgcagc      840

ccccaccaca cagccctgag gcaggccatc ctctgttggg gcgagctgat gaccctggcc      900

acctgggtgg gcaataacct ggaggacccc gccagcaggg acctggtggt caactacgtg      960

aacaccaaca tgggcctgaa gatcaggcag ctgctgtggt tccacatcag ctgcctgacc     1020

tttggcaggg agaccgtcct ggagtacctg gtgagcttcg gcgtgtggat caggactccc     1080

ccagcctaca ggccccctaa cgcccccatc ctgtctaccc tgcccgagac caccgtggtg     1140

aggaggaggg acaggggcag aagccccagg agaaggaccc ctagccccag gaggaggagg     1200

agccagagcc ccaggaggag gaggagccag agccgggaga gccagtgcgc ccctgtgaag     1260

cagaccctga acttcgacct gctgaagctg gccggcgacg tggagagcaa tcccggccct     1320

atggaaaaca tcaccagcgg cttcctgggc cccctgctgg tgctgcaggc cggcttcttc     1380

ctgctgacca ggatcctgac cattccccag tcactggaca gctggtggac cagcctgaac     1440

ttcctcggcg ggagccccgt gtgcctgggc cagaatagcc agagccccac cagcaaccac     1500

tctcccactt cctgcccccc tatctgcccc ggctacaggt ggatgtgcct gaggaggttc     1560

atcatcttcc tgttcatcct gctgctgtgc ctgatcttcc tgctggtgct gctggactac     1620

cagggaatgc tgcccgtgtg tcccctgatc cccggaagca ccaccaccaa caccggcccc     1680

tgcaagacct gcaccacccc cgcccagggc aactctatgt tccccagctg ctgctgcacc     1740

aagcccaccg acggcaactg cacttgcatt cccatcccca gcagctgggc cttcgccaaa     1800

tatctgtggg agtgggccag cgtgaggttt agctggctga gcctgctggt gcccttcgtg     1860

cagtggtttg tgggcctgag ccccaccgtg tggctgagcg ccatctggat gatgtggtac     1920

tggggcccct ccctgtacag catcgtgagc cccttcatcc ccctcctgcc catcttcttc     1980

tgcctgtggg tgtacatc                                                   1998


<210>  11
<211>  185
<212>  PRT
<213>  Hepatitis B virus

<400>  11

Met Asp Ile Asp Pro Tyr Lys Glu Phe Gly Ala Thr Val Glu Leu Leu 
1               5                   10                  15      


Ser Phe Leu Pro Ser Asp Phe Phe Pro Ser Val Arg Asp Leu Leu Asp 
            20                  25                  30          


Thr Ala Ser Ala Leu Tyr Arg Glu Ala Leu Glu Ser Pro Glu His Cys 
        35                  40                  45              


Ser Pro His His Thr Ala Leu Arg Gln Ala Ile Leu Cys Trp Gly Glu 
    50                  55                  60                  


Leu Met Thr Leu Ala Thr Trp Val Gly Asn Asn Leu Glu Asp Pro Ala 
65                  70                  75                  80  


Ser Arg Asp Leu Val Val Asn Tyr Val Asn Thr Asn Met Gly Leu Lys 
                85                  90                  95      


Ile Arg Gln Leu Leu Trp Phe His Ile Ser Cys Leu Thr Phe Gly Arg 
            100                 105                 110         


Glu Thr Val Leu Glu Tyr Leu Val Ser Phe Gly Val Trp Ile Arg Thr 
        115                 120                 125             


Pro Pro Ala Tyr Arg Pro Pro Asn Ala Pro Ile Leu Ser Thr Leu Pro 
    130                 135                 140                 


Glu Thr Thr Val Val Arg Arg Arg Asp Arg Gly Arg Ser Pro Arg Arg 
145                 150                 155                 160 


Arg Thr Pro Ser Pro Arg Arg Arg Arg Ser Gln Ser Pro Arg Arg Arg 
                165                 170                 175     


Arg Ser Gln Ser Arg Glu Ser Gln Cys 
            180                 185 


<210>  12
<211>  231
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of hIi alternate variant

<400>  12

Met His Arg Arg Arg Ser Arg Ser Cys Arg Glu Asp Gln Lys Pro Val 
1               5                   10                  15      


Met Asp Asp Gln Arg Asp Leu Ile Ser Asn Asn Glu Gln Leu Pro Met 
            20                  25                  30          


Leu Gly Arg Arg Pro Gly Ala Pro Glu Ser Lys Cys Ser Arg Gly Ala 
        35                  40                  45              


Leu Tyr Thr Gly Phe Ser Ile Leu Val Thr Leu Leu Leu Ala Gly Gln 
    50                  55                  60                  


Ala Thr Thr Ala Tyr Phe Leu Tyr Gln Gln Gln Gly Arg Leu Asp Lys 
65                  70                  75                  80  


Leu Thr Val Thr Ser Gln Asn Leu Gln Leu Glu Asn Leu Arg Met Lys 
                85                  90                  95      


Leu Pro Lys Pro Pro Lys Pro Val Ser Lys Met Arg Met Ala Thr Pro 
            100                 105                 110         


Leu Leu Met Gln Ala Leu Pro Met Gly Ala Leu Pro Gln Gly Pro Met 
        115                 120                 125             


Gln Asn Ala Thr Lys Tyr Gly Asn Met Thr Glu Asp His Val Met His 
    130                 135                 140                 


Leu Leu Gln Asn Ala Asp Pro Leu Lys Val Tyr Pro Pro Leu Lys Gly 
145                 150                 155                 160 


Ser Phe Pro Glu Asn Leu Arg His Leu Lys Asn Thr Met Glu Thr Ile 
                165                 170                 175     


Asp Trp Lys Val Phe Glu Ser Trp Met His His Trp Leu Leu Phe Glu 
            180                 185                 190         


Met Ser Arg His Ser Leu Glu Gln Lys Pro Thr Asp Ala Pro Pro Lys 
        195                 200                 205             


Glu Ser Leu Glu Leu Glu Asp Pro Ser Ser Gly Leu Gly Val Thr Lys 
    210                 215                 220                 


Gln Asp Leu Gly Pro Val Pro 
225                 230     


<210>  13
<211>  693
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence encoding hI alternate variant

<400>  13
atgcacagga ggagaagcag gagctgtcgg gaagatcaga agccagtcat ggatgaccag       60

cgcgacctta tctccaacaa tgagcaactg cccatgctgg gccggcgccc tggggccccg      120

gagagcaagt gcagccgcgg agccctgtac acaggctttt ccatcctggt gactctgctc      180

ctcgctggcc aggccaccac cgcctacttc ctgtaccagc agcagggccg gctggacaaa      240

ctgacagtca cctcccagaa cctgcagctg gagaacctgc gcatgaagct tcccaagcct      300

cccaagcctg tgagcaagat gcgcatggcc accccgctgc tgatgcaggc gctgcccatg      360

ggagccctgc cccaggggcc catgcagaat gccaccaagt atggcaacat gacagaggac      420

catgtgatgc acctgctcca gaatgctgac cccctgaagg tgtacccgcc actgaagggg      480

agcttcccgg agaacctgag acaccttaag aacaccatgg agaccataga ctggaaggtc      540

tttgagagct ggatgcacca ttggctcctg tttgaaatga gcaggcactc cttggagcaa      600

aagcccactg acgctccacc gaaagagtca ctggaactgg aggacccgtc ttctgggctg      660

ggtgtgacca agcaggatct gggcccagtc ccc                                   693


<210>  14
<211>  1998
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Alternative nucleic acid sequence of hIi-HBc-2A-HBs

<400>  14
atgcacagga ggagaagcag gagctgtcgg gaagatcaga agccagtcat ggatgaccag       60

cgcgacctta tctccaacaa tgagcaactg cccatgctgg gccggcgccc tggggccccg      120

gagagcaagt gcagccgcgg agccctgtac acaggctttt ccatcctggt gactctgctc      180

ctcgctggcc aggccaccac cgcctacttc ctgtaccagc agcagggccg gctggacaaa      240

ctgacagtca cctcccagaa cctgcagctg gagaacctgc gcatgaagct tcccaagcct      300

cccaagcctg tgagcaagat gcgcatggcc accccgctgc tgatgcaggc gctgcccatg      360

ggagccctgc cccaggggcc catgcagaat gccaccaagt atggcaacat gacagaggac      420

catgtgatgc acctgctcca gaatgctgac cccctgaagg tgtacccgcc actgaagggg      480

agcttcccgg agaacctgag acaccttaag aacaccatgg agaccataga ctggaaggtc      540

tttgagagct ggatgcacca ttggctcctg tttgaaatga gcaggcactc cttggagcaa      600

aagcccactg acgctccacc gaaagagtca ctggaactgg aggacccgtc ttctgggctg      660

ggtgtgacca agcaggatct gggcccagtc cccatggaca ttgaccctta taaagaattt      720

ggagctactg tggagttact ctcgtttttg ccttctgact tctttccttc cgtcagagat      780

ctcctagaca ccgcctcagc tctgtatcga gaagccttag agtctcctga gcattgctca      840

cctcaccata ctgcactcag gcaagccatt ctctgctggg gggaattgat gactctagct      900

acctgggtgg gtaataattt ggaagatcca gcatccaggg atctagtagt caattatgtt      960

aatactaaca tgggtttaaa gatcaggcaa ctattgtggt ttcatatatc ttgccttact     1020

tttggaagag agactgtact tgaatatttg gtctctttcg gagtgtggat tcgcactcct     1080

ccagcctata gaccaccaaa tgcccctatc ttatcaacac ttccggaaac tactgttgtt     1140

agacgacggg accgaggcag gtcccctaga agaagaactc cctcgcctcg cagacgcaga     1200

tctcaatcgc cgcgtcgcag aagatctcaa tctcgggaat ctcaatgtgc ccctgtgaag     1260

cagaccctga acttcgacct gctgaagctg gccggcgacg tggagagcaa tcccggccct     1320

atggagaaca tcacatcagg attcctagga cccctgctcg tgttacaggc ggggtttttc     1380

ttgttgacaa gaatcctcac aataccgcag agtctagact cgtggtggac ttctctcaat     1440

tttctagggg gatcacccgt gtgtcttggc caaaattcgc agtccccaac ctccaatcac     1500

tcaccaacct cctgtcctcc aatttgtcct ggttatcgct ggatgtgtct gcggcgtttt     1560

atcatattcc tcttcatcct gctgctatgc ctcatcttct tattggttct tctggattat     1620

caaggtatgt tgcccgtttg tcctctaatt ccaggatcaa caacaaccaa tacgggacca     1680

tgcaaaacct gcacgactcc tgctcaaggc aactctatgt ttccctcatg ttgctgtaca     1740

aaacctacgg atggaaattg cacctgtatt cccatcccat cgtcctgggc tttcgcaaaa     1800

tacctatggg agtgggcctc agtccgtttc tcttggctca gtttactagt gccatttgtt     1860

cagtggttcg tagggctttc ccccactgtt tggctttcag ctatatggat gatgtggtat     1920

tgggggccaa gtctgtacag catcgtgagt ccctttatac cgctgttacc aattttcttt     1980

tgtctctggg tatacatt                                                   1998


<210>  15
<211>  666
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Alternative amino acid sequence of hIi-HBc-2A-HBs

<400>  15

Met His Arg Arg Arg Ser Arg Ser Cys Arg Glu Asp Gln Lys Pro Val 
1               5                   10                  15      


Met Asp Asp Gln Arg Asp Leu Ile Ser Asn Asn Glu Gln Leu Pro Met 
            20                  25                  30          


Leu Gly Arg Arg Pro Gly Ala Pro Glu Ser Lys Cys Ser Arg Gly Ala 
        35                  40                  45              


Leu Tyr Thr Gly Phe Ser Ile Leu Val Thr Leu Leu Leu Ala Gly Gln 
    50                  55                  60                  


Ala Thr Thr Ala Tyr Phe Leu Tyr Gln Gln Gln Gly Arg Leu Asp Lys 
65                  70                  75                  80  


Leu Thr Val Thr Ser Gln Asn Leu Gln Leu Glu Asn Leu Arg Met Lys 
                85                  90                  95      


Leu Pro Lys Pro Pro Lys Pro Val Ser Lys Met Arg Met Ala Thr Pro 
            100                 105                 110         


Leu Leu Met Gln Ala Leu Pro Met Gly Ala Leu Pro Gln Gly Pro Met 
        115                 120                 125             


Gln Asn Ala Thr Lys Tyr Gly Asn Met Thr Glu Asp His Val Met His 
    130                 135                 140                 


Leu Leu Gln Asn Ala Asp Pro Leu Lys Val Tyr Pro Pro Leu Lys Gly 
145                 150                 155                 160 


Ser Phe Pro Glu Asn Leu Arg His Leu Lys Asn Thr Met Glu Thr Ile 
                165                 170                 175     


Asp Trp Lys Val Phe Glu Ser Trp Met His His Trp Leu Leu Phe Glu 
            180                 185                 190         


Met Ser Arg His Ser Leu Glu Gln Lys Pro Thr Asp Ala Pro Pro Lys 
        195                 200                 205             


Glu Ser Leu Glu Leu Glu Asp Pro Ser Ser Gly Leu Gly Val Thr Lys 
    210                 215                 220                 


Gln Asp Leu Gly Pro Val Pro Met Asp Ile Asp Pro Tyr Lys Glu Phe 
225                 230                 235                 240 


Gly Ala Thr Val Glu Leu Leu Ser Phe Leu Pro Ser Asp Phe Phe Pro 
                245                 250                 255     


Ser Val Arg Asp Leu Leu Asp Thr Ala Ser Ala Leu Tyr Arg Glu Ala 
            260                 265                 270         


Leu Glu Ser Pro Glu His Cys Ser Pro His His Thr Ala Leu Arg Gln 
        275                 280                 285             


Ala Ile Leu Cys Trp Gly Glu Leu Met Thr Leu Ala Thr Trp Val Gly 
    290                 295                 300                 


Asn Asn Leu Glu Asp Pro Ala Ser Arg Asp Leu Val Val Asn Tyr Val 
305                 310                 315                 320 


Asn Thr Asn Met Gly Leu Lys Ile Arg Gln Leu Leu Trp Phe His Ile 
                325                 330                 335     


Ser Cys Leu Thr Phe Gly Arg Glu Thr Val Leu Glu Tyr Leu Val Ser 
            340                 345                 350         


Phe Gly Val Trp Ile Arg Thr Pro Pro Ala Tyr Arg Pro Pro Asn Ala 
        355                 360                 365             


Pro Ile Leu Ser Thr Leu Pro Glu Thr Thr Val Val Arg Arg Arg Asp 
    370                 375                 380                 


Arg Gly Arg Ser Pro Arg Arg Arg Thr Pro Ser Pro Arg Arg Arg Arg 
385                 390                 395                 400 


Ser Gln Ser Pro Arg Arg Arg Arg Ser Gln Ser Arg Glu Ser Gln Cys 
                405                 410                 415     


Ala Pro Val Lys Gln Thr Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly 
            420                 425                 430         


Asp Val Glu Ser Asn Pro Gly Pro Met Glu Asn Ile Thr Ser Gly Phe 
        435                 440                 445             


Leu Gly Pro Leu Leu Val Leu Gln Ala Gly Phe Phe Leu Leu Thr Arg 
    450                 455                 460                 


Ile Leu Thr Ile Pro Gln Ser Leu Asp Ser Trp Trp Thr Ser Leu Asn 
465                 470                 475                 480 


Phe Leu Gly Gly Ser Pro Val Cys Leu Gly Gln Asn Ser Gln Ser Pro 
                485                 490                 495     


Thr Ser Asn His Ser Pro Thr Ser Cys Pro Pro Ile Cys Pro Gly Tyr 
            500                 505                 510         


Arg Trp Met Cys Leu Arg Arg Phe Ile Ile Phe Leu Phe Ile Leu Leu 
        515                 520                 525             


Leu Cys Leu Ile Phe Leu Leu Val Leu Leu Asp Tyr Gln Gly Met Leu 
    530                 535                 540                 


Pro Val Cys Pro Leu Ile Pro Gly Ser Thr Thr Thr Asn Thr Gly Pro 
545                 550                 555                 560 


Cys Lys Thr Cys Thr Thr Pro Ala Gln Gly Asn Ser Met Phe Pro Ser 
                565                 570                 575     


Cys Cys Cys Thr Lys Pro Thr Asp Gly Asn Cys Thr Cys Ile Pro Ile 
            580                 585                 590         


Pro Ser Ser Trp Ala Phe Ala Lys Tyr Leu Trp Glu Trp Ala Ser Val 
        595                 600                 605             


Arg Phe Ser Trp Leu Ser Leu Leu Val Pro Phe Val Gln Trp Phe Val 
    610                 615                 620                 


Gly Leu Ser Pro Thr Val Trp Leu Ser Ala Ile Trp Met Met Trp Tyr 
625                 630                 635                 640 


Trp Gly Pro Ser Leu Tyr Ser Ile Val Ser Pro Phe Ile Pro Leu Leu 
                645                 650                 655     


Pro Ile Phe Phe Cys Leu Trp Val Tyr Ile 
            660                 665     


<210>  16
<211>  3182
<212>  DNA
<213>  Hepatitis B virus

<400>  16
aattccacaa cctttcacca aactctgcaa gatcccagag tgagaggcct gtatttccct       60

gctggtggct ccagttcagg agcagtaaac cctgttccga ctactgcctc tcccttatcg      120

tcaatcttct cgaggattgg ggaccctgcg ctgaacatgg agaacatcac atcaggattc      180

ctaggacccc ttctcgtgtt acaggcgggg tttttcttgt tgacaagaat cctcacaata      240

ccgcagagtc tagactcgtg gtggacttct ctcaattttc tagggggaac taccgtgtgt      300

cttggccaaa attcgcagtc cccaacctcc aatcactcac caacctcctg tcctccaact      360

tgtcctggtt atcgctggat gtgtctgcgg cgttttatca tcttcctctt catcctgctg      420

ctatgcctca tcttcttgtt ggttcttctg gactatcaag gtatgttgcc cgtttgtcct      480

ctaattccag gatcctcaac caccagcacg ggaccatgcc gaacctgcat gactactgct      540

caaggaacct ctatgtatcc ctcctgttgc tgtaccaaac cttcggacgg aaattgcacc      600

tgtattccca tcccatcatc ctgggctttc ggaaaattcc tatgggagtg ggcctcagcc      660

cgtttctcct ggctcagttt actagtgcca tttgttcagt ggttcgtagg gctttccccc      720

actgtttggc tttcagttat atggatgatg tggtattggg ggccaagtct gtacagcatc      780

ttgagtccct ttttaccgct gttaccaatt ttcttttgtc tttgggtata catttaaacc      840

ctaacaaaac aaagagatgg ggttactctc tgaattttat gggttatgtc attggaagtt      900

atgggtcctt gccacaagaa cacatcatac aaaaaatcaa agaatgtttt agaaaacttc      960

ctattaacag gcctattgat tggaaagtat gtcaacgaat tgtgggtctt ttgggttttg     1020

ctgccccatt tacacaatgt ggttatcctg cgttaatgcc cttgtatgca tgtattcaat     1080

ctaagcaggc tttcactttc tcgccaactt acaaggcctt tctgtgtaaa caatacctga     1140

acctttaccc cgttgcccgg caacggccag gtctgtgcca agtgtttgct gacgcaaccc     1200

ccactggctg gggcttggtc atgggccatc agcgcgtgcg tggaaccttt tcggctcctc     1260

tgccgatcca tactgcggaa ctcctagccg cttgttttgc tcgcagcagg tctggagcaa     1320

acattatcgg gactgataac tctgttgtcc tctcccgcaa atatacatcg tatccatggc     1380

tgctaggctg tgctgccaac tggatcctgc gcgggacgtc ctttgtttac gtcccgtcgg     1440

cgctgaatcc tgcggacgac ccttctcggg gtcgcttggg actctctcgt ccccttctcc     1500

gtctgccgtt ccgaccgacc acggggcgca cctctcttta cgcggactcc ccgtctgtgc     1560

cttctcatct gccggaccgt gtgcacttcg cttcacctct gcacgtcgca tggagaccac     1620

cgtgaacgcc caccgaatgt tgcccaaggt cttacataag aggactcttg gactctctgc     1680

aatgtcaacg accgaccttg aggcatactt caaagactgt ttgtttaaag actgggagga     1740

gttgggggag gagattagat taaaggtctt tgtactagga ggctgtaggc ataaattggt     1800

ctgcgcacca gcaccatgca actttttcac ctctgcctaa tcatctcttg ttcatgtcct     1860

actgttcaag cctccaagct gtgccttggg tggctttggg gcatggacat cgacccttat     1920

aaagaatttg gagctactgt ggagttactc tcgtttttgc cttctgactt ctttccttca     1980

gtacgagatc ttctagatac cgcctcagct ctgtatcggg aagccttaga gtctcctgag     2040

cattgttcac ctcaccatac tgcactcagg caagcaattc tttgctgggg ggaactaatg     2100

actctagcta cctgggtggg tgttaatttg gaagatccag catctagaga cctagtagtc     2160

agttatgtca acactaatat gggcctaaag ttcaggcaac tcttgtggtt tcacatttct     2220

tgtctcactt ttggaagaga aaccgttata gagtatttgg tgtctttcgg agtgtggatt     2280

cgcactcctc cagcttatag accaccaaat gcccctatcc tatcaacact tccggaaact     2340

actgttgtta gacgacgagg caggtcccct agaagaagaa ctccctcgcc tcgcagacga     2400

aggtctcaat cgccgcgtcg cagaagatct caatctcggg aacctcaatg ttagtattcc     2460

ttggactcat aaggtgggga actttactgg tctttattct tctactgtac ctgtctttaa     2520

tcctcattgg aaaacaccat cttttcctaa tatacattta caccaagaca ttatcaaaaa     2580

atgtgaacag tttgtaggcc cacttacagt taatgagaaa agaagattgc aattgattat     2640

gcctgctagg ttttatccaa aggttaccaa atatttacca ttggataagg gtattaaacc     2700

ttattatcca gaacatctag ttaatcatta cttccaaact agacactatt tacacactct     2760

atggaaggcg ggtatattat ataagagaga aacaacacat agcgcctcat tttgtgggtc     2820

accatattct tgggaacaag atctacagca tggggcagaa tctttccacc agcaatcctc     2880

tgggattctt tcccgaccac cagttggatc cagccttcag agcaaacaca gcaaatccag     2940

attgggactt caatcccaac aaggacacct ggccagacgc caacaaggta ggagctggag     3000

cattcgggct gggtttcacc ccaccgcacg gaggcctttt ggggtggagc cctcaggctc     3060

agggcatact acaaactttg ccagcaaatc cgcctcctgc ctccaccaat cgccagacag     3120

gaaggcagcc taccccgctg tctccacctt tgagaaacac tcatcctcag gccatgcagt     3180

gg                                                                    3182


<210>  17
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Part of viral primer probe set RTS3370

<400>  17
cttggtcatg ggccatcag                                                    19


<210>  18
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Part of viral primer probe set RTS3370

<400>  18
cggctaggag ttccgcagta                                                   20


<210>  19
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Part of viral primer probe set RTS3370

<400>  19
tgcgtggaac cttttcggct cc                                                22


<210>  20
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Part of viral primer probe set RTS3371

<400>  20
ccaaaccttc ggacggaaa                                                    19


<210>  21
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Part of viral primer probe set RTS3371

<400>  21
tgaggcccac tcccatagg                                                    19


<210>  22
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Part of viral primer probe set RTS3371

<400>  22
cccatcatcc tgggctttcg gaaaat                                            26


<210>  23
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  : Part of viral primer probe set RTS3372

<400>  23
atcctatcaa cacttccgga aact                                              24


<210>  24
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Part of viral primer probe set RTS3372

<400>  24
cgacgcggcg attgag                                                       16


<210>  25
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Part of viral primer probe set RTS3372

<400>  25
aagaactccc tcgcctcgca gacg                                              24


<210>  26
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Part of viral primer probe set RTS3373MGB

<400>  26
ccgaccttga ggcatacttc a                                                 21


<210>  27
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Part of viral primer probe set RTS3373MGB

<400>  27
aatttatgcc tacagcctcc tagtaca                                           27


<210>  28
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Part of viral primer probe set RTS3373MGB

<400>  28
ttaaagactg ggaggagttg                                                   20


