                         SEQUENCE LISTING

<110>  DING, Beibei
       GUO, Wenzhong
       ZHANG, Yanliang
 
<120>  IMPROVED PROCESS FOR DNA INTEGRATION USING RNA-GUIDED 
       ENDONUCLEASES

<130>  087735.0103

<140>  US 16/288,052
<141>  2019-02-27

<150>  US 62/635,702
<151>  2018-02-27

<160>  43    

<170>  PatentIn version 3.5

<210>  1
<211>  19
<212>  DNA
<213>  Homo sapiens


<220>
<221>  misc_feature
<222>  (1)..(19)
<223>  Target sequence in TRAC gene

<400>  1
cagggttctg gatatctgt                                                    19


<210>  2
<211>  2119
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: anti-CD38 CAR Construct for insertion into TRAC locus


<220>
<221>  misc_feature
<223>  JeT promoter, DNA sequence encoding CD8a leader peptide, 
       anti-CD38 CAR (single chain variable fragment (scFv) specific for
       human CD38), CD28 hinge-transmembrane-intracellular regions and 
       CD3 zeta intracellular domain

<400>  2
gaattcgggc ggagttaggg cggagccaat cagcgtgcgc cgttccgaaa gttgcctttt       60

atggctgggc ggagaatggg cggtgaacgc cgatgattat ataaggacgc gccgggtgtg      120

gcacagctag ttccgtcgca gccgggattt gggtcgcggt tcttgtttgt ggatccctgt      180

gatcgtcact tgacagtaag tcactgactg tctatgcctg ggaaagggtg ggcaggagat      240

ggggcagtgc aggaaaagtg gcactatgaa ccctgcagcc ctaggaatgc atctagacaa      300

ttgtactaac cttcttctct ttcctctcct gacaggcctc gaggccgcca ccatggaatg      360

gtcatgggtc tttctctttt ttctcagcgt gaccaccgga gtccactccc aggtagagca      420

gaaattgatc tctgaggaag acctgcaggt ccagttggtc gaaagtggcg gcggattggt      480

gaaaccaggc ggatctttga ggcttagttg cgcggcttcc ggatttacgt tcagtgatga      540

ctacatgagc tggataaggc aagcacctgg taagggcctg gaatgggtcg caagtgtgtc      600

taatggaagg cccactacct actatgctga ttccgtccgc ggacgcttta ctatttcaag      660

agataatgct aagaatagtc tgtacctgca gatgaacagt ctgcgcgcgg aagataccgc      720

agtatattac tgtgcacgag aggattgggg tggggagttc acggattggg gcaggggaac      780

tcttgtaacg gtgtctagcg gaggaggtgg gtcaggtgga ggtggcagtg gaggtggagg      840

ctctcaggcc ggcttgaccc aaccgccatc tgcgtcagga acatcaggcc agagggtgac      900

tatcagttgt tctggcagtt catccaatat tgggatcaat ttcgtgtact ggtatcagca      960

cctgccaggt accgcgccga agctgctgat ctataagaat aatcaacgcc catcaggcgt     1020

tccagatagg ttcagtggga gcaagtccgg aaactccgcg tcactcgcga tctcaggtct     1080

gcggtctgag gatgaagctg attattactg cgcggcgtgg gatgattctc tgtcaggcta     1140

cgtattcggt tcagggacta aggtaactgt gttggcgaaa ccgaccacga caccggctcc     1200

aagacctccg acgccagctc caacgatagc gtcacagcca ttgtctctcc gccctgaagc     1260

ctgccggccc gctgcgggcg gcgcggttca tacccgggga ttggactttg cccccagaaa     1320

gatagaggtg atgtaccctc ccccctactt ggacaacgaa aagtctaatg gcactatcat     1380

tcacgtaaag ggcaaacacc tttgtccaag tcctttgttc ccaggcccat ctaagccgtt     1440

ctgggtactc gtggttgtgg ggggcgtgct cgcttgttac tcactgctgg tgacggtggc     1500

ctttattatt ttctgggttc gatctaagcg aagccgcttg ttgcattctg actacatgaa     1560

tatgacgcca agacggccag ggccaacaag aaagcattac caaccgtacg cccccccgcg     1620

agacttcgcg gcctaccgca gcagggtaaa atttagcagg tctgcagatg cgcctgcgta     1680

tcaacagggt cagaatcagc tctataatga gctgaacctc gggcggcggg aagagtatga     1740

tgttctcgat aaaaggagag gacgagaccc cgaaatgggc ggcaaaccga gacgcaaaaa     1800

tcctcaggag gggctctaca atgaacttca aaaagacaaa atggccgaag catactcaga     1860

aatcggaatg aaaggggaga ggagacgcgg gaagggccat gatggactgt atcagggact     1920

ttccacggcc accaaggaca cctatgacgc tctccacatg caggcgctgc cgcctagatg     1980

ataaaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata     2040

gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca     2100

aactcatcaa tgtatctta                                                  2119


<210>  3
<211>  195
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Jet promoter

<400>  3
gaattcgggc ggagttaggg cggagccaat cagcgtgcgc cgttccgaaa gttgcctttt       60

atggctgggc ggagaatggg cggtgaacgc cgatgattat ataaggacgc gccgggtgtg      120

gcacagctag ttccgtcgca gccgggattt gggtcgcggt tcttgtttgt ggatccctgt      180

gatcgtcagt tgaca                                                       195


<210>  4
<211>  3429
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: anti-CD38A2 CAR cassette with homology arms

<400>  4
ggcaccatat tcattttgca ggtgaaattc ctgagatgta aggagctgct gtgacttgct       60

caaggcctta tatcgagtaa acggtagtgc tggggcttag acgcaggtgt tctgatttat      120

agttcaaaac ctctatcaat gagagagcaa tctcctggta atgtgataga tttcccaact      180

taatgccaac ataccataaa cctcccattc tgctaatgcc cagcctaagt tggggagacc      240

actccagatt ccaagatgta cagtttgctt tgctgggcct ttttcccatg cctgccttta      300

ctctgccaga gttatattgc tggggttttg aagaagatcc tattaaataa aagaataagc      360

agtattatta agtagccctg catttcaggt ttccttgagt ggcaggccag gcctggccgt      420

gaacgttcac tgaaatcatg gcctcttggc caagattgat agcttgtgcc tgtccctgag      480

tcccagtcca tcacgagcag ctggtttcta agatgctatt tcccgtataa agcatgagac      540

cgtgacttgc cagccccaca gagccccgcc cttgtccatc actggcatct ggactccagc      600

ctgggttggg gcaaagaggg aaatgagatc atgtcctaac cctgatcctc ttgtcccaca      660

gaattcgggc ggagttaggg cggagccaat cagcgtgcgc cgttccgaaa gttgcctttt      720

atggctgggc ggagaatggg cggtgaacgc cgatgattat ataaggacgc gccgggtgtg      780

gcacagctag ttccgtcgca gccgggattt gggtcgcggt tcttgtttgt ggatccctgt      840

gatcgtcact tgacagtaag tcactgactg tctatgcctg ggaaagggtg ggcaggagat      900

ggggcagtgc aggaaaagtg gcactatgaa ccctgcagcc ctaggaatgc atctagacaa      960

ttgtactaac cttcttctct ttcctctcct gacaggcctc gaggccgcca ccatggaatg     1020

gtcatgggtc tttctctttt ttctcagcgt gaccaccgga gtccactccc aggtagagca     1080

gaaattgatc tctgaggaag acctgcaggt ccagttggtc gaaagtggcg gcggattggt     1140

gaaaccaggc ggatctttga ggcttagttg cgcggcttcc ggatttacgt tcagtgatga     1200

ctacatgagc tggataaggc aagcacctgg taagggcctg gaatgggtcg caagtgtgtc     1260

taatggaagg cccactacct actatgctga ttccgtccgc ggacgcttta ctatttcaag     1320

agataatgct aagaatagtc tgtacctgca gatgaacagt ctgcgcgcgg aagataccgc     1380

agtatattac tgtgcacgag aggattgggg tggggagttc acggattggg gcaggggaac     1440

tcttgtaacg gtgtctagcg gaggaggtgg gtcaggtgga ggtggcagtg gaggtggagg     1500

ctctcaggcc ggcttgaccc aaccgccatc tgcgtcagga acatcaggcc agagggtgac     1560

tatcagttgt tctggcagtt catccaatat tgggatcaat ttcgtgtact ggtatcagca     1620

cctgccaggt accgcgccga agctgctgat ctataagaat aatcaacgcc catcaggcgt     1680

tccagatagg ttcagtggga gcaagtccgg aaactccgcg tcactcgcga tctcaggtct     1740

gcggtctgag gatgaagctg attattactg cgcggcgtgg gatgattctc tgtcaggcta     1800

cgtattcggt tcagggacta aggtaactgt gttggcgaaa ccgaccacga caccggctcc     1860

aagacctccg acgccagctc caacgatagc gtcacagcca ttgtctctcc gccctgaagc     1920

ctgccggccc gctgcgggcg gcgcggttca tacccgggga ttggactttg cccccagaaa     1980

gatagaggtg atgtaccctc ccccctactt ggacaacgaa aagtctaatg gcactatcat     2040

tcacgtaaag ggcaaacacc tttgtccaag tcctttgttc ccaggcccat ctaagccgtt     2100

ctgggtactc gtggttgtgg ggggcgtgct cgcttgttac tcactgctgg tgacggtggc     2160

ctttattatt ttctgggttc gatctaagcg aagccgcttg ttgcattctg actacatgaa     2220

tatgacgcca agacggccag ggccaacaag aaagcattac caaccgtacg cccccccgcg     2280

agacttcgcg gcctaccgca gcagggtaaa atttagcagg tctgcagatg cgcctgcgta     2340

tcaacagggt cagaatcagc tctataatga gctgaacctc gggcggcggg aagagtatga     2400

tgttctcgat aaaaggagag gacgagaccc cgaaatgggc ggcaaaccga gacgcaaaaa     2460

tcctcaggag gggctctaca atgaacttca aaaagacaaa atggccgaag catactcaga     2520

aatcggaatg aaaggggaga ggagacgcgg gaagggccat gatggactgt atcagggact     2580

ttccacggcc accaaggaca cctatgacgc tctccacatg caggcgctgc cgcctagatg     2640

ataaaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata     2700

gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca     2760

aactcatcaa tgtatcttag atatccagaa ccctgaccct gccgtgtacc agctgagaga     2820

ctctaaatcc agtgacaagt ctgtctgcct attcaccgat tttgattctc aaacaaatgt     2880

gtcacaaagt aaggattctg atgtgtatat cacagacaaa actgtgctag acatgaggtc     2940

tatggacttc aagagcaaca gtgctgtggc ctggagcaac aaatctgact ttgcatgtgc     3000

aaacgccttc aacaacagca ttattccaga ggacaccttc ttccccagcc caggtaaggg     3060

cagctttggt gccttcgcag gctgtttcct tgcttcagga atggccaggt tctgcccaga     3120

gctctggtca atgatgtcta aaactcctct gattggtggt ctcggcctta tccattgcca     3180

ccaaaaccct ctttttacta agaaacagtg agccttgttc tggcagtcca gagaatgaca     3240

cgggaaaaaa gcagatgaag agaaggtggc aggagagggc acgtggccca gcctcagtct     3300

ctccaactga gttcctgcct gcctgccttt gctcagactg tttgcccctt actgctcttc     3360

taggcctcat tctaagcccc ttctccaagt tgcctctcct tatttctccc tgtctgccaa     3420

aaaatcttt                                                             3429


<210>  5
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: primer for sequencing clones with anti-CD38 CAR-TRAC 
       homology arms insert in pAAV-MCS vector

<400>  5
cttaggctgg gcattagcag                                                   20


<210>  6
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: primer for sequencing clones with anti-CD38 CAR-TRAC 
       homology arms insert in pAAV-MCS vector

<400>  6
catggaatgg tcatgggtct                                                   20


<210>  7
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: primer for sequencing clones with anti-CD38 CAR-TRAC 
       homology arms insert in pAAV-MCS vector

<400>  7
ggctacgtat tcggttcagg                                                   20


<210>  8
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Forward primer for generating donor DNA PCR fragment 
       from pAAV anti-CD38 CAR-TRAC construct (660 & 650 HAs)


<220>
<221>  modified_base
<222>  (1)..(2)
<223>  Phosphorothioate linkage

<220>
<221>  modified_base
<222>  (2)..(3)
<223>  Phosphorothioate linkage

<220>
<221>  modified_base
<222>  (2)..(3)
<223>  2'-O-methylated deoxyguanosine

<220>
<221>  modified_base
<222>  (3)..(4)
<223>  Phosphorothioate linkage

<220>
<221>  modified_base
<222>  (4)..(4)
<223>  2'-O-methylated deoxyadenosine

<400>  8
tggagctagg gcaccatatt                                                   20


<210>  9
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Reverse primer for generating donor DNA PCR fragment 
       from pAAV anti-CD38 CAR-TRAC construct (660 & 650 HAs), the 
       5-most nucleoside (C) has a 5 phosphate

<400>  9
caacttggag aaggggctta                                                   20


<210>  10
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: PCR forward primer with homology to TRAC locus for 
       verifying site-specific insertion of anti-CD38 CAR (upstream 
       junction)

<400>  10
ctgctttctg agggtgaag                                                    19


<210>  11
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: PCR reverse primer with homology to CAR construct for 
       verifying site-specific insertion of anti-CD38 CAR (upstream 
       junction)

<400>  11
ctttcgacca actggacctg                                                   20


<210>  12
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: PCR forward primer with homology to CAR locus for 
       verifying site-specific insertion of anti-CD38 CAR (downstream 
       junction)

<400>  12
cgttctgggt actcgtggtt                                                   20


<210>  13
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: PCR reverse primer with homology to TRAC locus 
       (downstream junction)

<400>  13
gagagccctt ccctgacttt                                                   20


<210>  14
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Forward primer for generating donor fragment with 300 
       nt HAs


<220>
<221>  modified_base
<222>  (1)..(2)
<223>  Phosphorothioate linkage

<220>
<221>  modified_base
<222>  (2)..(3)
<223>  Phosphorothioate linkage

<220>
<221>  modified_base
<222>  (2)..(2)
<223>  2-O-methylated deoxycytosine

<220>
<221>  modified_base
<222>  (3)..(4)
<223>  Phosphorothioate linkage

<220>
<221>  modified_base
<222>  (3)..(3)
<223>  2'-O-methylated deoxyadenosine

<220>
<221>  modified_base
<222>  (5)..(5)
<223>  2'-O-methylated deoxyguanosine

<400>  14
ccatgcctgc ctttactctg                                                   20


<210>  15
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Reverse primer for generating donor fragment with 300 
       nt HAs, the 5-most nucleoside (T) has a 5 phosphate

<400>  15
tcctgaagca aggaaacagc                                                   20


<210>  16
<211>  375
<212>  DNA
<213>  Homo sapiens


<220>
<221>  misc_feature
<222>  (1)..(375)
<223>  5 homology arm, exon 1 TRAC gene

<400>  16
ccatgcctgc ctttactctg ccagagttat attgctgggg ttttgaagaa gatcctatta       60

aataaaagaa taagcagtat tattaagtag ccctgcattt caggtttcct tgagtggcag      120

gccaggcctg gccgtgaacg ttcactgaaa tcatggcctc ttggccaaga ttgatagctt      180

gtgcctgtcc ctgagtccca gtccatcacg agcagctggt ttctaagatg ctatttcccg      240

tataaagcat gagaccgtga cttgccagcc ccacagagcc ccgcccttgt ccatcactgg      300

catctggact ccagcctggg ttggggcaaa gagggaaatg agatcatgtc ctaaccctga      360

tcctcttgtc ccaca                                                       375


<210>  17
<211>  321
<212>  DNA
<213>  Homo sapiens


<220>
<221>  misc_feature
<222>  (1)..(321)
<223>  3 homology arm, exon 1 TRAC gene

<400>  17
gatatccaga accctgaccc tgccgtgtac cagctgagag actctaaatc cagtgacaag       60

tctgtctgcc tattcaccga ttttgattct caaacaaatg tgtcacaaag taaggattct      120

gatgtgtata tcacagacaa aactgtgcta gacatgaggt ctatggactt caagagcaac      180

agtgctgtgg cctggagcaa caaatctgac tttgcatgtg caaacgcctt caacaacagc      240

attattccag aggacacctt cttccccagc ccaggtaagg gcagctttgg tgccttcgca      300

ggctgtttcc ttgcttcagg a                                                321


<210>  18
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Forward primer for generating donor fragment with 150 
       nt HAs


<220>
<221>  modified_base
<222>  (1)..(2)
<223>  Phosphorothioate linkage

<220>
<221>  modified_base
<222>  (3)..(4)
<223>  Phosphorothioate linkage

<220>
<221>  modified_base
<222>  (3)..(3)
<223>  2-O-methylated deoxycytosine

<220>
<221>  modified_base
<222>  (4)..(5)
<223>  Phosphorothioate linkage

<220>
<221>  modified_base
<222>  (4)..(4)
<223>  2'-O-methylated deoxyadenosine

<220>
<221>  modified_base
<222>  (5)..(5)
<223>  2-O-methylated deoxycytosine

<400>  18
atcacgagca gctggtttct                                                   20


<210>  19
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Reverse primer for generating donor fragment with 150 
       nt HAs, the 5-most nucleoside (G) has a 5 phosphate

<400>  19
gacctcatgt ctagcacagt tttg                                              24


<210>  20
<211>  171
<212>  DNA
<213>  Homo sapiens


<220>
<221>  misc_feature
<222>  (1)..(171)
<223>  5 171 nt homology arm, exon 1 TRAC gene

<400>  20
atcacgagca gctggtttct aagatgctat ttcccgtata aagcatgaga ccgtgacttg       60

ccagccccac agagccccgc ccttgtccat cactggcatc tggactccag cctgggttgg      120

ggcaaagagg gaaatgagat catgtcctaa ccctgatcct cttgtcccac a               171


<210>  21
<211>  161
<212>  DNA
<213>  Homo sapiens


<220>
<221>  misc_feature
<222>  (1)..(161)
<223>  3 161 nt homology arm, exon 1 TRAC gene

<400>  21
gatatccaga accctgaccc tgccgtgtac cagctgagag actctaaatc cagtgacaag       60

tctgtctgcc tattcaccga ttttgattct caaacaaatg tgtcacaaag taaggattct      120

gatgtgtata tcacagacaa aactgtgcta gacatgaggt c                          161


<210>  22
<211>  2080
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: anti-CD19 CAR cassette including JeT promoter, intron,
       anti-CD19 CAR, SV40 sequence

<400>  22
gaattcgggc ggagttaggg cggagccaat cagcgtgcgc cgttccgaaa gttgcctttt       60

atggctgggc ggagaatggg cggtgaacgc cgatgattat ataaggacgc gccgggtgtg      120

gcacagctag ttccgtcgca gccgggattt gggtcgcggt tcttgtttgt ggatccctgt      180

gatcgtcact tgacagtaag tcactgactg tctatgcctg ggaaagggtg ggcaggagat      240

ggggcagtgc aggaaaagtg gcactatgaa ccctgcagcc ctaggaatgc atctagacaa      300

ttgtactaac cttcttctct ttcctctcct gacaggcctc gaggccgcca ccatggaatg      360

gtcatgggtc tttctctttt ttctcagcgt gaccaccgga gtccactccg atatccagat      420

gacacagacc accagcagcc tgagcgccag cctgggcgac cgagtgacta tcagctgccg      480

ggcatcccag gatatttcta agtatctgaa ctggtaccag cagaagcccg acggcactgt      540

caaactgctg atctaccaca ccagtagact gcattcaggg gtgcctagca ggttctccgg      600

atctggcagt gggactgact actccctgac catctctaac ctggagcagg aagatattgc      660

cacctatttc tgccagcagg gcaatacact gccttacact tttggcgggg gaacaaagct      720

ggagatcact ggcggaggag gatctggagg aggaggaagt ggaggaggag gatcagaggt      780

gaaactgcag gaaagcggac caggactggt cgcaccttca cagagcctgt ccgtgacatg      840

tactgtctcc ggagtgtctc tgcccgatta cggcgtctct tggatccggc agccccctag      900

aaagggactg gagtggctgg gcgtgatctg gggaagtgaa actacctact ataatagtgc      960

tctgaaatca agactgacca tcattaagga caactctaaa agtcaggtgt ttctgaagat     1020

gaattccctg cagaccgacg atacagcaat ctactattgc gccaaacact actattacgg     1080

cgggagctat gccatggatt actgggggca gggaacttcc gtcaccgtga gcagcgctaa     1140

gccgaccacg acaccggctc caagacctcc gacgccagct ccaacgatag cgtcacagcc     1200

attgtctctc cgccctgaag cctgccggcc cgctgcgggc ggcgcggttc atacccgggg     1260

attggacttt gcccccagaa agatagaggt gatgtaccct cccccctact tggacaacga     1320

aaagtctaat ggcactatca ttcacgtaaa gggcaaacac ctttgtccaa gtcctttgtt     1380

cccaggccca tctaagccgt tctgggtact cgtggttgtg gggggcgtgc tcgcttgtta     1440

ctcactgctg gtgacggtgg cctttattat tttctgggtt cgatctaagc gaagccgctt     1500

gttgcattct gactacatga atatgacgcc aagacggcca gggccaacaa gaaagcatta     1560

ccaaccgtac gcccccccgc gagacttcgc ggcctaccgc agcagggtaa aatttagcag     1620

gtctgcagat gcgcctgcgt atcaacaggg tcagaatcag ctctataatg agctgaacct     1680

cgggcggcgg gaagagtatg atgttctcga taaaaggaga ggacgagacc ccgaaatggg     1740

cggcaaaccg agacgcaaaa atcctcagga ggggctctac aatgaacttc aaaaagacaa     1800

aatggccgaa gcatactcag aaatcggaat gaaaggggag aggagacgcg ggaagggcca     1860

tgatggactg tatcagggac tttccacagc caccaaggac acctatgacg ctctccacat     1920

gcaggcgctg ccgcctagat gataaaattg ttgttgttaa cttgtttatt gcagcttata     1980

atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc     2040

attctagttg tggtttgtcc aaactcatca atgtatctta                           2080


<210>  23
<211>  2083
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: anti-BCMA CAR construct including JeT promoter, 
       intron, anti-BCMA CAR construct, SV40 sequence


<220>
<221>  misc_feature
<222>  (1057)..(1057)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (1098)..(1098)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (1120)..(1120)
<223>  n is a, c, g, or t

<400>  23
gaattcgggc ggagttaggg cggagccaat cagcgtgcgc cgttccgaaa gttgcctttt       60

atggctgggc ggagaatggg cggtgaacgc cgatgattat ataaggacgc gccgggtgtg      120

gcacagctag ttccgtcgca gccgggattt gggtcgcggt tcttgtttgt ggatccctgt      180

gatcgtcact tgacagtaag tcactgactg tctatgcctg ggaaagggtg ggcaggagat      240

ggggcagtgc aggaaaagtg gcactatgaa ccctgcagcc ctaggaatgc atctagacaa      300

ttgtactaac cttcttctct ttcctctcct gacaggcctc gaggccgcca ccatggagtg      360

gtcctgggtg ttcctgttct ttctgtccgt gaccaccggt gtccactctc aggtgcagct      420

ggtggagtct gggggaggct tggtaaagcc tggggggtcc cttagactct cctgtgcagc      480

ctctggattc acttccagta ccgcctggat gagctgggtc cgccaggctc cagggaaggg      540

gctggagtgg gttggccgta ttaaaagcaa aagtgatggt gggacaacag actacgctgc      600

acccgtgaaa ggcagattca ccatctcaag agatgattca aaaaacacgc tgtttctgca      660

aatgaacagc ctgaaaaccg aggacacagc cgtgtattac tgtgccaagg gaggcgggac      720

ctacggctac tggggccagg gaaccctggt caccgtctcc tccggcggcg gcggcagcgg      780

tggcggtggc tcaggtggtg gtggttcttc ctatgtgctg actcagcctg cctccgtgtc      840

tgggtctcct ggacagtcag tcaccatctc ctgcactgga accagcagtg atggtggtgg      900

tcacacctat gtctcctggt accaacagca cccaggcaaa gcccccaaac tcatgattta      960

tgatgtcagt aatcggccct catgggtttc taatcgcttc tctggctcca agtctggcaa     1020

cacggcctcc ctgaccatct ctgggctcca ggctgangac gaggctgatt attactgcgg     1080

ctcatataca agcagcgnct cttatgtctt cggaactggn accaagctga ccgtcctggc     1140

taagcccacc acgacgccag cgccgcgacc accaacaccg gcgcccacca tcgcgtcgca     1200

gcccctgtcc ctgcgcccag aggcgtgccg gccagcggcg gggggcgcag tgcacacgag     1260

ggggctggac ttcgccccta ggaaaattga agttatgtat cctcctcctt acctagacaa     1320

tgagaagagc aatggaacca ttatccatgt gaaagggaaa cacctttgtc caagtcccct     1380

atttcccgga ccttctaagc ccttttgggt gctggtggtg gttggtggag tcctggcttg     1440

ctatagcttg ctagtaacag tggcctttat tattttctgg gtgaggagta agaggagcag     1500

gctcctgcac agtgactaca tgaacatgac tccccgccgc cccgggccca cccgcaagca     1560

ttaccagccc tatgccccac cacgcgactt cgcagcctat cgctccagag tgaagttcag     1620

caggagcgca gacgcccccg cgtaccagca gggccagaac cagctctata acgagctcaa     1680

tctaggacga agagaggagt acgatgtttt ggacaagaga cgtggccggg accctgagat     1740

ggggggaaag ccgagaagga agaaccctca ggaaggcctg tacaatgaac tgcagaaaga     1800

taagatggcg gaggcctaca gtgagattgg gatgaaaggc gagcgccgga ggggcaaggg     1860

gcacgatggc ctttaccagg gtctcagtac agccaccaag gacacctacg acgcccttca     1920

catgcaggcc ctgccgccta gatgataaaa ttgttgttgt taacttgttt attgcagctt     1980

ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac     2040

tgcattctag ttgtggtttg tccaaactca tcaatgtatc tta                       2083


<210>  24
<211>  183
<212>  DNA
<213>  Homo sapiens


<220>
<221>  misc_feature
<222>  (1)..(183)
<223>  5 homology arm, TRAC gene exon 3, 183 nt

<400>  24
tatgcacaga agctgcaagg gacaggaggt gcaggagctg caggcctccc ccacccagcc       60

tgctctgcct tggggaaaac cgtgggtgtg tcctgcaggc catgcaggcc tgggacatgc      120

aagcccataa ccgctgtggc ctcttggttt tacagatacg aacctaaact ttcaaaacct      180

gtc                                                                    183


<210>  25
<211>  140
<212>  DNA
<213>  Homo sapiens


<220>
<221>  misc_feature
<222>  (1)..(140)
<223>  3 homology arm, TRAC gene exon 3

<400>  25
agtgattggg ttccgaatcc tcctcctgaa agtggccggg tttaatctgc tcatgacgct       60

gcggctgtgg tccagctgag gtgaggggcc ttgaagctgg gagtggggtt tagggacgcg      120

ggtctctggg tgcatcctaa                                                  140


<210>  26
<211>  20
<212>  DNA
<213>  Homo sapiens


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  Exon 3 target sequence (guide sequence)

<400>  26
ttcggaaccc aatcactgac                                                   20


<210>  27
<211>  2442
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Entire donor DNA anti-CD38 CAR plus exon 3 HAs on both
       ends

<400>  27
tatgcacaga agctgcaagg gacaggaggt gcaggagctg caggcctccc ccacccagcc       60

tgctctgcct tggggaaaac cgtgggtgtg tcctgcaggc catgcaggcc tgggacatgc      120

aagcccataa ccgctgtggc ctcttggttt tacagatacg aacctaaact ttcaaaacct      180

gtcgaattcg ggcggagtta gggcggagcc aatcagcgtg cgccgttccg aaagttgcct      240

tttatggctg ggcggagaat gggcggtgaa cgccgatgat tatataagga cgcgccgggt      300

gtggcacagc tagttccgtc gcagccggga tttgggtcgc ggttcttgtt tgtggatccc      360

tgtgatcgtc acttgacagt aagtcactga ctgtctatgc ctgggaaagg gtgggcagga      420

gatggggcag tgcaggaaaa gtggcactat gaaccctgca gccctaggaa tgcatctaga      480

caattgtact aaccttcttc tctttcctct cctgacaggc ctcgaggccg ccaccatgga      540

atggtcatgg gtctttctct tttttctcag cgtgaccacc ggagtccact cccaggtaga      600

gcagaaattg atctctgagg aagacctgca ggtccagttg gtcgaaagtg gcggcggatt      660

ggtgaaacca ggcggatctt tgaggcttag ttgcgcggct tccggattta cgttcagtga      720

tgactacatg agctggataa ggcaagcacc tggtaagggc ctggaatggg tcgcaagtgt      780

gtctaatgga aggcccacta cctactatgc tgattccgtc cgcggacgct ttactatttc      840

aagagataat gctaagaata gtctgtacct gcagatgaac agtctgcgcg cggaagatac      900

cgcagtatat tactgtgcac gagaggattg gggtggggag ttcacggatt ggggcagggg      960

aactcttgta acggtgtcta gcggaggagg tgggtcaggt ggaggtggca gtggaggtgg     1020

aggctctcag gccggcttga cccaaccgcc atctgcgtca ggaacatcag gccagagggt     1080

gactatcagt tgttctggca gttcatccaa tattgggatc aatttcgtgt actggtatca     1140

gcacctgcca ggtaccgcgc cgaagctgct gatctataag aataatcaac gcccatcagg     1200

cgttccagat aggttcagtg ggagcaagtc cggaaactcc gcgtcactcg cgatctcagg     1260

tctgcggtct gaggatgaag ctgattatta ctgcgcggcg tgggatgatt ctctgtcagg     1320

ctacgtattc ggttcaggga ctaaggtaac tgtgttggcg aaaccgacca cgacaccggc     1380

tccaagacct ccgacgccag ctccaacgat agcgtcacag ccattgtctc tccgccctga     1440

agcctgccgg cccgctgcgg gcggcgcggt tcatacccgg ggattggact ttgcccccag     1500

aaagatagag gtgatgtacc ctccccccta cttggacaac gaaaagtcta atggcactat     1560

cattcacgta aagggcaaac acctttgtcc aagtcctttg ttcccaggcc catctaagcc     1620

gttctgggta ctcgtggttg tggggggcgt gctcgcttgt tactcactgc tggtgacggt     1680

ggcctttatt attttctggg ttcgatctaa gcgaagccgc ttgttgcatt ctgactacat     1740

gaatatgacg ccaagacggc cagggccaac aagaaagcat taccaaccgt acgccccccc     1800

gcgagacttc gcggcctacc gcagcagggt aaaatttagc aggtctgcag atgcgcctgc     1860

gtatcaacag ggtcagaatc agctctataa tgagctgaac ctcgggcggc gggaagagta     1920

tgatgttctc gataaaagga gaggacgaga ccccgaaatg ggcggcaaac cgagacgcaa     1980

aaatcctcag gaggggctct acaatgaact tcaaaaagac aaaatggccg aagcatactc     2040

agaaatcgga atgaaagggg agaggagacg cgggaagggc catgatggac tgtatcaggg     2100

actttccacg gccaccaagg acacctatga cgctctccac atgcaggcgc tgccgcctag     2160

atgataaaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca     2220

atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt     2280

ccaaactcat caatgtatct taagtgattg ggttccgaat cctcctcctg aaagtggccg     2340

ggtttaatct gctcatgacg ctgcggctgt ggtccagctg aggtgagggg ccttgaagct     2400

gggagtgggg tttagggacg cgggtctctg ggtgcatcct aa                        2442


<210>  28
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Forward primer for generating donor fragment of SEQ ID
       NO:27


<220>
<221>  modified_base
<222>  (1)..(2)
<223>  Phosphorothioate linkage

<220>
<221>  modified_base
<222>  (2)..(3)
<223>  Phosphorothioate linkage

<220>
<221>  modified_base
<222>  (2)..(2)
<223>  2'-O-methylated deoxyadenosine

<220>
<221>  modified_base
<222>  (3)..(4)
<223>  Phosphorothioate linkage

<220>
<221>  modified_base
<222>  (4)..(4)
<223>  2'-O-methylated deoxyguanosine

<220>
<221>  modified_base
<222>  (5)..(5)
<223>  2-O-methylated deoxycytosine

<400>  28
tatgccacag aagctgcaag g                                                 21


<210>  29
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Reverse primer for generating donor fragment of SEQ ID
       NO: 27


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5-most nucleoside (T) has a 5 phosphate

<400>  29
ttaggatgca cccagagacc                                                   20


<210>  30
<211>  326
<212>  DNA
<213>  Homo sapiens


<220>
<221>  misc_feature
<222>  (1)..(326)
<223>  PD-1 locus 5 HA

<400>  30
ctccccatct cctctgtctc cctgtctctg tctctctctc cctcccccac cctctcccca       60

gtcctacccc ctcctcaccc ctcctccccc agcactgcct ctgtcactct cgcccacgtg      120

gatgtggagg aagagggggc gggagcaagg ggcgggcacc ctcccttcaa cctgacctgg      180

gacagtttcc cttccgctca cctccgcctg agcagtggag aaggcggcac tctggtgggg      240

ctgctccagg catgcagatc ccacaggcgc cctggccagt cgtctgggcg gtgctacaac      300

tgggctggcg gccaggatgg ttctta                                           326


<210>  31
<211>  380
<212>  DNA
<213>  Homo sapiens


<220>
<221>  misc_feature
<222>  (1)..(380)
<223>  PD-1 locus 3 HA

<400>  31
ggtaggtggg gtcggcggtc aggtgtccca gagccagggg tctggaggga ccttccaccc       60

tcagtccctg gcaggtcggg gggtgctgag gcgggcctgg ccctggcagc ccaggggtcc      120

cggagcgagg ggtctggagg gacctttcac tctcagtccc tggcaggtcg gggggtgctg      180

tggcaggccc agccttggcc cccagctctg ccccttaccc tgagctgtgt ggctttgggc      240

agctcgaact cctgggttcc tctctgggcc ccaactcctc ccctggccca agtcccctct      300

ttgctcctgg gcaggcagga cctctgtccc ctctcagccg gtccttgggg ctgcgtgttt      360

ctgtagaatg acgggtcagg                                                  380


<210>  32
<211>  20
<212>  DNA
<213>  Homo sapiens


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  PD-1 target site

<400>  32
ggccaggatg gttcttaggt                                                   20


<210>  33
<211>  2825
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Donor DNA fragment having CD38 cassette (SEQ ID NO:2) 
       flanked by PD-1 HAs

<400>  33
ctccccatct cctctgtctc cctgtctctg tctctctctc cctcccccac cctctcccca       60

gtcctacccc ctcctcaccc ctcctccccc agcactgcct ctgtcactct cgcccacgtg      120

gatgtggagg aagagggggc gggagcaagg ggcgggcacc ctcccttcaa cctgacctgg      180

gacagtttcc cttccgctca cctccgcctg agcagtggag aaggcggcac tctggtgggg      240

ctgctccagg catgcagatc ccacaggcgc cctggccagt cgtctgggcg gtgctacaac      300

tgggctggcg gccaggatgg ttcttagaat tcgggcggag ttagggcgga gccaatcagc      360

gtgcgccgtt ccgaaagttg ccttttatgg ctgggcggag aatgggcggt gaacgccgat      420

gattatataa ggacgcgccg ggtgtggcac agctagttcc gtcgcagccg ggatttgggt      480

cgcggttctt gtttgtggat ccctgtgatc gtcacttgac agtaagtcac tgactgtcta      540

tgcctgggaa agggtgggca ggagatgggg cagtgcagga aaagtggcac tatgaaccct      600

gcagccctag gaatgcatct agacaattgt actaaccttc ttctctttcc tctcctgaca      660

ggcctcgagg ccgccaccat ggaatggtca tgggtctttc tcttttttct cagcgtgacc      720

accggagtcc actcccaggt agagcagaaa ttgatctctg aggaagacct gcaggtccag      780

ttggtcgaaa gtggcggcgg attggtgaaa ccaggcggat ctttgaggct tagttgcgcg      840

gcttccggat ttacgttcag tgatgactac atgagctgga taaggcaagc acctggtaag      900

ggcctggaat gggtcgcaag tgtgtctaat ggaaggccca ctacctacta tgctgattcc      960

gtccgcggac gctttactat ttcaagagat aatgctaaga atagtctgta cctgcagatg     1020

aacagtctgc gcgcggaaga taccgcagta tattactgtg cacgagagga ttggggtggg     1080

gagttcacgg attggggcag gggaactctt gtaacggtgt ctagcggagg aggtgggtca     1140

ggtggaggtg gcagtggagg tggaggctct caggccggct tgacccaacc gccatctgcg     1200

tcaggaacat caggccagag ggtgactatc agttgttctg gcagttcatc caatattggg     1260

atcaatttcg tgtactggta tcagcacctg ccaggtaccg cgccgaagct gctgatctat     1320

aagaataatc aacgcccatc aggcgttcca gataggttca gtgggagcaa gtccggaaac     1380

tccgcgtcac tcgcgatctc aggtctgcgg tctgaggatg aagctgatta ttactgcgcg     1440

gcgtgggatg attctctgtc aggctacgta ttcggttcag ggactaaggt aactgtgttg     1500

gcgaaaccga ccacgacacc ggctccaaga cctccgacgc cagctccaac gatagcgtca     1560

cagccattgt ctctccgccc tgaagcctgc cggcccgctg cgggcggcgc ggttcatacc     1620

cggggattgg actttgcccc cagaaagata gaggtgatgt accctccccc ctacttggac     1680

aacgaaaagt ctaatggcac tatcattcac gtaaagggca aacacctttg tccaagtcct     1740

ttgttcccag gcccatctaa gccgttctgg gtactcgtgg ttgtgggggg cgtgctcgct     1800

tgttactcac tgctggtgac ggtggccttt attattttct gggttcgatc taagcgaagc     1860

cgcttgttgc attctgacta catgaatatg acgccaagac ggccagggcc aacaagaaag     1920

cattaccaac cgtacgcccc cccgcgagac ttcgcggcct accgcagcag ggtaaaattt     1980

agcaggtctg cagatgcgcc tgcgtatcaa cagggtcaga atcagctcta taatgagctg     2040

aacctcgggc ggcgggaaga gtatgatgtt ctcgataaaa ggagaggacg agaccccgaa     2100

atgggcggca aaccgagacg caaaaatcct caggaggggc tctacaatga acttcaaaaa     2160

gacaaaatgg ccgaagcata ctcagaaatc ggaatgaaag gggagaggag acgcgggaag     2220

ggccatgatg gactgtatca gggactttcc acggccacca aggacaccta tgacgctctc     2280

cacatgcagg cgctgccgcc tagatgataa aattgttgtt gttaacttgt ttattgcagc     2340

ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc     2400

actgcattct agttgtggtt tgtccaaact catcaatgta tcttaggtag gtggggtcgg     2460

cggtcaggtg tcccagagcc aggggtctgg agggaccttc caccctcagt ccctggcagg     2520

tcggggggtg ctgaggcggg cctggccctg gcagcccagg ggtcccggag cgaggggtct     2580

ggagggacct ttcactctca gtccctggca ggtcgggggg tgctgtggca ggcccagcct     2640

tggcccccag ctctgcccct taccctgagc tgtgtggctt tgggcagctc gaactcctgg     2700

gttcctctct gggccccaac tcctcccctg gcccaagtcc cctctttgct cctgggcagg     2760

caggacctct gtcccctctc agccggtcct tggggctgcg tgtttctgta gaatgacggg     2820

tcagg                                                                 2825


<210>  34
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Forward primer for generating donor fragment of SEQ ID
       NO:33


<220>
<221>  misc_feature
<223>  5-most nucleoside (C) has a 5 phosphate

<400>  34
ctccccatct cctctgtctc                                                   20


<210>  35
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Reverse primer for generating donor fragment of SEQ ID
       NO:33


<220>
<221>  modified_base
<222>  (1)..(2)
<223>  Phosphorothioate linkage

<220>
<221>  modified_base
<222>  (1)..(1)
<223>  2-O-methylated deoxycytosine

<220>
<221>  modified_base
<222>  (2)..(3)
<223>  Phosphorothioate linkage

<220>
<221>  modified_base
<222>  (2)..(2)
<223>  2-O-methylated deoxycytosine

<220>
<221>  modified_base
<222>  (3)..(4)
<223>  Phosphorothioate linkage

<220>
<221>  modified_base
<222>  (4)..(4)
<223>  2'-O-methylated deoxyguanosine

<400>  35
cctggacccg tcattctaca g                                                 21


<210>  36
<211>  20
<212>  DNA
<213>  Homo sapiens


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  Forward primer for generating donor DNA PCR fragment from pAAV 
       anti-CD38 CAR-TRAC construct (660 & 650 HAs)

<400>  36
tggagctagg gcaccatatt                                                   20


<210>  37
<211>  2415
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: anti-BCMA CAR construct donor fragment sequence, 
       including JeT promoter, intron, anti-BCMA CAR construct, SV40 
       sequence, with 5 and 3 TRAC gene exon 1 homology arms


<220>
<221>  misc_feature
<222>  (1228)..(1228)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (1269)..(1269)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (1291)..(1291)
<223>  n is a, c, g, or t

<400>  37
atcacgagca gctggtttct aagatgctat ttcccgtata aagcatgaga ccgtgacttg       60

ccagccccac agagccccgc ccttgtccat cactggcatc tggactccag cctgggttgg      120

ggcaaagagg gaaatgagat catgtcctaa ccctgatcct cttgtcccac agaattcggg      180

cggagttagg gcggagccaa tcagcgtgcg ccgttccgaa agttgccttt tatggctggg      240

cggagaatgg gcggtgaacg ccgatgatta tataaggacg cgccgggtgt ggcacagcta      300

gttccgtcgc agccgggatt tgggtcgcgg ttcttgtttg tggatccctg tgatcgtcac      360

ttgacagtaa gtcactgact gtctatgcct gggaaagggt gggcaggaga tggggcagtg      420

caggaaaagt ggcactatga accctgcagc cctaggaatg catctagaca attgtactaa      480

ccttcttctc tttcctctcc tgacaggcct cgaggccgcc accatggagt ggtcctgggt      540

gttcctgttc tttctgtccg tgaccaccgg tgtccactct caggtgcagc tggtggagtc      600

tgggggaggc ttggtaaagc ctggggggtc ccttagactc tcctgtgcag cctctggatt      660

cacttccagt accgcctgga tgagctgggt ccgccaggct ccagggaagg ggctggagtg      720

ggttggccgt attaaaagca aaagtgatgg tgggacaaca gactacgctg cacccgtgaa      780

aggcagattc accatctcaa gagatgattc aaaaaacacg ctgtttctgc aaatgaacag      840

cctgaaaacc gaggacacag ccgtgtatta ctgtgccaag ggaggcggga cctacggcta      900

ctggggccag ggaaccctgg tcaccgtctc ctccggcggc ggcggcagcg gtggcggtgg      960

ctcaggtggt ggtggttctt cctatgtgct gactcagcct gcctccgtgt ctgggtctcc     1020

tggacagtca gtcaccatct cctgcactgg aaccagcagt gatggtggtg gtcacaccta     1080

tgtctcctgg taccaacagc acccaggcaa agcccccaaa ctcatgattt atgatgtcag     1140

taatcggccc tcatgggttt ctaatcgctt ctctggctcc aagtctggca acacggcctc     1200

cctgaccatc tctgggctcc aggctganga cgaggctgat tattactgcg gctcatatac     1260

aagcagcgnc tcttatgtct tcggaactgg naccaagctg accgtcctgg ctaagcccac     1320

cacgacgcca gcgccgcgac caccaacacc ggcgcccacc atcgcgtcgc agcccctgtc     1380

cctgcgccca gaggcgtgcc ggccagcggc ggggggcgca gtgcacacga gggggctgga     1440

cttcgcccct aggaaaattg aagttatgta tcctcctcct tacctagaca atgagaagag     1500

caatggaacc attatccatg tgaaagggaa acacctttgt ccaagtcccc tatttcccgg     1560

accttctaag cccttttggg tgctggtggt ggttggtgga gtcctggctt gctatagctt     1620

gctagtaaca gtggccttta ttattttctg ggtgaggagt aagaggagca ggctcctgca     1680

cagtgactac atgaacatga ctccccgccg ccccgggccc acccgcaagc attaccagcc     1740

ctatgcccca ccacgcgact tcgcagccta tcgctccaga gtgaagttca gcaggagcgc     1800

agacgccccc gcgtaccagc agggccagaa ccagctctat aacgagctca atctaggacg     1860

aagagaggag tacgatgttt tggacaagag acgtggccgg gaccctgaga tggggggaaa     1920

gccgagaagg aagaaccctc aggaaggcct gtacaatgaa ctgcagaaag ataagatggc     1980

ggaggcctac agtgagattg ggatgaaagg cgagcgccgg aggggcaagg ggcacgatgg     2040

cctttaccag ggtctcagta cagccaccaa ggacacctac gacgcccttc acatgcaggc     2100

cctgccgcct agatgataaa attgttgttg ttaacttgtt tattgcagct tataatggtt     2160

acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta     2220

gttgtggttt gtccaaactc atcaatgtat cttagatatc cagaaccctg accctgccgt     2280

gtaccagctg agagactcta aatccagtga caagtctgtc tgcctattca ccgattttga     2340

ttctcaaaca aatgtgtcac aaagtaagga ttctgatgtg tatatcacag acaaaactgt     2400

gctagacatg aggtc                                                      2415


<210>  38
<211>  2217
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: anti-CD19 CAR cassette including JeT promoter, intron,
       anti-CD19 CAR, SV40 sequence flanked by 5 and 3 TRAC gene exon 1 
       homology arms of SEQ ID NO:20 and SEQ ID NO:21

<400>  38
atcacgagca gctggtttct aagatgctat ttcccgtata aagcatgaga ccgtgacttg       60

ccagccccac agagccccgc ccttgtccat cactggcatc tggactccag cctgggttgg      120

ggcaaagagg gaaatgagat catgtcctaa ccctgatcct cttgtcccac agtaagtcac      180

tgactgtcta tgcctgggaa agggtgggca ggagatgggg cagtgcagga aaagtggcac      240

tatgaaccct gcagccctag gaatgcatct agacaattgt actaaccttc ttctctttcc      300

tctcctgaca ggcctcgagg ccgccaccat ggaatggtca tgggtctttc tcttttttct      360

cagcgtgacc accggagtcc actccgatat ccagatgaca cagaccacca gcagcctgag      420

cgccagcctg ggcgaccgag tgactatcag ctgccgggca tcccaggata tttctaagta      480

tctgaactgg taccagcaga agcccgacgg cactgtcaaa ctgctgatct accacaccag      540

tagactgcat tcaggggtgc ctagcaggtt ctccggatct ggcagtggga ctgactactc      600

cctgaccatc tctaacctgg agcaggaaga tattgccacc tatttctgcc agcagggcaa      660

tacactgcct tacacttttg gcgggggaac aaagctggag atcactggcg gaggaggatc      720

tggaggagga ggaagtggag gaggaggatc agaggtgaaa ctgcaggaaa gcggaccagg      780

actggtcgca ccttcacaga gcctgtccgt gacatgtact gtctccggag tgtctctgcc      840

cgattacggc gtctcttgga tccggcagcc ccctagaaag ggactggagt ggctgggcgt      900

gatctgggga agtgaaacta cctactataa tagtgctctg aaatcaagac tgaccatcat      960

taaggacaac tctaaaagtc aggtgtttct gaagatgaat tccctgcaga ccgacgatac     1020

agcaatctac tattgcgcca aacactacta ttacggcggg agctatgcca tggattactg     1080

ggggcaggga acttccgtca ccgtgagcag cgctaagccg accacgacac cggctccaag     1140

acctccgacg ccagctccaa cgatagcgtc acagccattg tctctccgcc ctgaagcctg     1200

ccggcccgct gcgggcggcg cggttcatac ccggggattg gactttgccc ccagaaagat     1260

agaggtgatg taccctcccc cctacttgga caacgaaaag tctaatggca ctatcattca     1320

cgtaaagggc aaacaccttt gtccaagtcc tttgttccca ggcccatcta agccgttctg     1380

ggtactcgtg gttgtggggg gcgtgctcgc ttgttactca ctgctggtga cggtggcctt     1440

tattattttc tgggttcgat ctaagcgaag ccgcttgttg cattctgact acatgaatat     1500

gacgccaaga cggccagggc caacaagaaa gcattaccaa ccgtacgccc ccccgcgaga     1560

cttcgcggcc taccgcagca gggtaaaatt tagcaggtct gcagatgcgc ctgcgtatca     1620

acagggtcag aatcagctct ataatgagct gaacctcggg cggcgggaag agtatgatgt     1680

tctcgataaa aggagaggac gagaccccga aatgggcggc aaaccgagac gcaaaaatcc     1740

tcaggagggg ctctacaatg aacttcaaaa agacaaaatg gccgaagcat actcagaaat     1800

cggaatgaaa ggggagagga gacgcgggaa gggccatgat ggactgtatc agggactttc     1860

cacagccacc aaggacacct atgacgctct ccacatgcag gcgctgccgc ctagatgata     1920

aaattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca     1980

tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac     2040

tcatcaatgt atcttagata tccagaaccc tgaccctgcc gtgtaccagc tgagagactc     2100

taaatccagt gacaagtctg tctgcctatt caccgatttt gattctcaaa caaatgtgtc     2160

acaaagtaag gattctgatg tgtatatcac agacaaaact gtgctagaca tgaggtc        2217


<210>  39
<211>  1001
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Sequenced PCR product of 5 end of donor DNA plus 
       adjacent TRAC gene exon 1 genomic sequence that included portion 
       of anti-CD38 CAR construct and 660 nt homology arm


<220>
<221>  misc_feature
<222>  (1)..(15)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (18)..(19)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (790)..(792)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (925)..(925)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (931)..(931)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (933)..(934)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (1001)..(1001)
<223>  n is a, c, g, or t

<400>  39
nnnnnnnnnn nnnnngcnng actcactagc actctatcac ggccatattc tggcagggtc       60

agtggctcca actaacattt gtttggtact ttacagttta ttaaatagat gtttatatgg      120

agaagctctc atttctttct cagaagagcc tggctaggaa ggtggatgag gcaccatatt      180

cattttgcag gtgaaattcc tgagatgtaa ggagctgctg tgacttgctc aaggccttat      240

atcgagtaaa cggtagcgct ggggcttaga cgcaggtgtt ctgatttata gttcaaaacc      300

tctatcaatg agagagcaat ctcctggtaa tgtgatagat ttcccaactt aatgccaaca      360

taccataaac ctcccattct gctaatgccc agcctaagtt ggggagacca ctccagattc      420

caagatgtac agtttgcttt gctgggcctt tttcccatgc ctgcctttac tctgccagag      480

ttatattgct ggggttttga agaagatcct attaaataaa agaataagca gtattattaa      540

gtagccctgc atttcaggtt tccttgagtg gcaggccagg cctggccgtg aacgttcact      600

gaaatcatgg cctcttggcc aagattgata gcttgtgcct gtccctgagt cccagtccat      660

cacgagcagc tggtttctaa gatgctattt cccgtataaa gcatgagacc gtgacttgcc      720

agccccacag agccccgccc ttgtccatca ctggcatctg gactccagcc tgggttgggg      780

caaagagggn nngagatcat gtcctaaccc tgatcctctt gtcccacaga attcgggcgg      840

agttagggcg gagccaatca gcgtgcgccg ttccgaaagt tgccttttat ggctgggcgg      900

agaatgggcg gtgaacgccg atgantatat nannacgcgc cgggtgtggc acagctagtt      960

ccgtcgcagc cgggatttgg gtcgcggttc ttgtttgtgg n                         1001


<210>  40
<211>  1015
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Sequenced PCR product of 3 end of donor DNA plus 
       adjacent TRAC gene exon 1 genomic sequence that included portion 
       of anti-CD38 CAR construct and 650 nt homology arm


<220>
<221>  misc_feature
<222>  (1)..(16)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (988)..(988)
<223>  n is a, c, g, or t

<400>  40
nnnnnnnnnn nnnnnnttct gctctacctg ggagtggact ccggtggtca cgctgagaaa       60

aaagagaaag acccatgacc attccatggt ggcggcctcg aggcctgtca ggagaggaaa      120

gagaagaagg ttagtacaat tgtctagatg cattcctagg gctgcagggt tcatagtgcc      180

acttttcctg cactgcccca tctcctgccc accctttccc aggcatagac agtcagtgac      240

ttactgtcaa gtgacgatca cagggatcca caaacaagaa ccgcgaccca aatcccggct      300

gcgacggaac tagctgtgcc acacccggcg cgtccttata taatcatcgg cgttcaccgc      360

ccattctccg cccagccata aaaggcaact ttcggaacgg cgcacgctga ttggctccgc      420

cctaactccg cccgaattct gtgggacaag aggatcaggg ttaggacatg atctcatttc      480

cctctttgcc ccaacccagg ctggagtcca gatgccagtg atggacaagg gcggggctct      540

gtggggctgg caagtcacgg tctcatgctt tatacgggaa atagcatctt agaaaccagc      600

tgctcgtgat ggactgggac tcagggacag gcacaagcta tcaatcttgg ccaagaggcc      660

atgatttcag tgaacgttca cggccaggcc tggcctgcca ctcaaggaaa cctgaaatgc      720

agggctactt aataatactg cttattcttt tatttaatag gatcttcttc aaaaccccag      780

caatataact ctggcagagt aaaggcaggc atgggaaaaa ggcccagcaa agcaaactgt      840

acatcttgga atctggagtg gtctccccaa cttaggctgg gcattagcag aatgggaggt      900

ttatggtatg ttggcattaa gttgggaaat ctatcacatt accaggagat tgctctctca      960

ttgatagagg ttttgaacta taaatcanaa cacctgcgtc taagccccag cgcta          1015


<210>  41
<211>  1035
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Sequenced PCR product of 5 end of donor DNA plus 
       adjacent TRAC gene exon 3 genomic sequence that included portion 
       of the anti-CD38 CAR construct and 660 nt homology arm; Exon3 5HA
       F sequence results


<220>
<221>  misc_feature
<222>  (2)..(21)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (31)..(31)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (1030)..(1030)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (1032)..(1035)
<223>  n is a, c, g, or t

<400>  41
cnnnnnnnnn nnnnnnnnnn nctttgagga ngagtttcta gcttcaatag accaaggact       60

ctctcctagg cctctgtatt cctttcaaca gctccactgt caagagagcc agagagagct      120

tctgggtggc ccagctgtga aatttctgag tcccttaggg atagccctaa acgaaccaga      180

tcatcctgag gacagccaag aggttttgcc ttctttcaag acaagcaaca gtactcacat      240

aggctgtggg caatggtcct gtctctcaag aatcccctgc cactcctcac acccaccctg      300

ggcccatatt catttccatt tgagttgttc ttattgagtc atccttcctg tggtagcgga      360

actcactaag gggcccatct ggacccgagg tattgtgatg ataaattctg agcacctacc      420

ccatccccag aagggctcag aaataaaata agagccaagt ctagtcggtg tttcctgtct      480

tgaaacacaa tactgttggc cctggaagaa tgcacagaat ctgtttgtaa ggggatatgc      540

acagaagctg caagggacag gaggtgcagg agctgcaggc ctcccccacc cagcctgctc      600

tgccttgggg aaaaccgtgg gtgtgtcctg caggccatgc aggcctggga catgcaagcc      660

cataaccgct gtggcctctt ggttttacag atacgaacct aaactttcaa aacctgtcga      720

attcgggcgg agttagggcg gagccaatca gcgtgcgccg ttccgaaagt tgccttttat      780

ggctgggcgg agaatgggcg gtgaacgccg atgattatat aaggacgcgc cgggtgtggc      840

acagctagtt ccgtcgcagc cgggatttgg gtcgcggttc ttgtttgtgg atccctgtga      900

tcgtcacttg acagtaagtc actgactgtc tatgcctggg aaagggtggg caggagatgg      960

ggcagtgcag gaaaagtggc actatgaacc ctgcagccct aggaatgcat ctagacaatt     1020

gtactaaccn tnnnn                                                      1035


<210>  42
<211>  1030
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Sequenced PCR product of 3 end of donor DNA plus 
       adjacent genomic TRAC gene exon 3 sequence that included portion 
       of the anti-CD38 CAR construct and 650 nt homology arm


<220>
<221>  misc_feature
<222>  (1)..(18)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (952)..(952)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (1007)..(1007)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (1030)..(1030)
<223>  n is a, c, g, or t

<400>  42
nnnnnnnnnn nnnnnnnngc tgcacaggag agtctcaggg accctccagg cttgaccaag       60

cctcccccag actccaccag ctgcacctga gagtggacac cggtggtcac ggacagaaag      120

aacaggaaca cccaggacca ctccatggtg gcggcctcga ggcctgtcag gagaggaaag      180

agaagaaggt tagtacaatt gtctagatgc attcctaggg ctgcagggtt catagtgcca      240

cttttcctgc actgccccat ctcctgccca ccctttccca ggcatagaca gtcagtgact      300

tactgtcaag tgacgatcac agggatccac aaacaagaac cgcgacccaa atcccggctg      360

cgacggaact agctgtgcca cacccggcgc gtccttatat aatcatcggc gttcaccgcc      420

cattctccgc ccagccataa aaggcaactt tcggaacggc gcacgctgat tggctccgcc      480

ctaactccgc ccgaattcga caggttttga aagtttaggt tcgtatctgt aaaaccaaga      540

ggccacagcg gttatgggct tgcatgtccc aggcctgcat ggcctgcagg acacacccac      600

ggttttcccc aaggcagagc aggctgggtg ggggaggcct gcagctcctg cacctcctgt      660

cccttgcagc ttctgtgcat atccccttac aaacagattc tgtgcattct tccagggcca      720

acagtattgt gtttcaagac aggaaacacc gactagactt ggctcttatt ttatttctga      780

gcccttctgg ggatggggta ggtgctcaga atttatcatc acaatacctc gggtccagat      840

gggcccctta gtgagttccg ctaccacagg aaggatgact caataagaac aactcaaatg      900

gaaatgaata tgggcccagg gtgggtgtga ggagtggcag gggattcttg anagacagga      960

ccattgccca cagcctatgt gagtactgtt gcttgtcttg aaagaangca aaacctcttg     1020

gctgtcctcn                                                            1030


<210>  43
<211>  1015
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Sequenced PCR product of 3 end of donor DNA plus 
       adjacent PD-1 genomic sequence that included portion of the 
       anti-CD38 CAR construct and 660 nt homology arm


<220>
<221>  misc_feature
<222>  (1)..(19)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (22)..(22)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (32)..(32)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (925)..(926)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (930)..(930)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (935)..(936)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (943)..(943)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (947)..(947)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (960)..(961)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (971)..(971)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (974)..(976)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (978)..(978)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (983)..(985)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (990)..(991)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (999)..(999)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (1012)..(1013)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (1015)..(1015)
<223>  n is a, c, g, or t

<400>  43
nnnnnnnnnn nnnnnnnnnt cntgctggtg anggtggcct ttattatttt ctgggttcga       60

tctaagcgaa gccgcttgtt gcattctgac tacatgaata tgacgccaag acggccaggg      120

ccaacaagaa agcattacca accgtacgcc cccccgcgag actacgcggc ctaccgcagc      180

agggtaaaat ttagcaggtc tgcagatgcg cctgcgtatc aacagggtca gaatcagctc      240

tataatgagc tgaacctcgg gcggcgggaa gagtatgatg ttctcgataa aaggagagga      300

cgagaccccg aaatgggcgg caaaccgaga cgcaaaaatc ctcaggaggg gctctacaat      360

gaacttcaaa aagacaaaat ggccgaagca tactcagaaa tcggaatgaa aggggagagg      420

agacgcggga agggccatga tggactgtat cagggacttt ccacagccac caaggacacc      480

tatgacgctc tccacatgca ggcgctgccg cctagatgat aaaattgttg ttgttaactt      540

gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa      600

agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttaggt      660

aggtggggtc ggcggtcagg tgtcccagag ccaggggtct ggagggacct tccaccctca      720

gtccctggca ggtcgggggg tgctgaggcg ggcctggccc tggcagccca ggggtcccgg      780

agcgaggggt ctggagggac ctttcactct cagtccctgg caggtcgggg ggtgctgtgg      840

caggcccagc cttggccccc agctctgccc cttaccctga gctgtgtggc tttgggcagc      900

tcaaactcct gggttcctct ctggnncccn actcnncccc tgncccnagt cccctctttn      960

ntcctgggca ngcnnnanct ctnnnccctn ncagccggnc cttggggctg cnngn          1015


