                         SEQUENCE LISTING

<110>  Northwestern University
       Jewett, Michael Christopher
       Kruger-Gericke, Antje
       Mueller, Alexander Paul
       Koepke, Michael
 
<120>  CELL-FREE PROTEIN SYNTHESIS PLATFORM FROM CLOSTRIDIA EXTRACTS

<130>  702581.01702

<150>  62/810,014
<151>  2019-02-25

<160>  30    

<170>  PatentIn version 3.5

<210>  1
<211>  235
<212>  PRT
<213>  E. coli

<400>  1

Met Tyr Arg Tyr Leu Ser Ile Ala Ala Val Val Leu Ser Ala Ala Phe 
1               5                   10                  15      


Ser Gly Pro Ala Leu Ala Glu Gly Ile Asn Ser Phe Ser Gln Ala Lys 
            20                  25                  30          


Ala Ala Ala Val Lys Val His Ala Asp Ala Pro Gly Thr Phe Tyr Cys 
        35                  40                  45              


Gly Cys Lys Ile Asn Trp Gln Gly Lys Lys Gly Val Val Asp Leu Gln 
    50                  55                  60                  


Ser Cys Gly Tyr Gln Val Arg Lys Asn Glu Asn Arg Ala Ser Arg Val 
65                  70                  75                  80  


Glu Trp Glu His Val Val Pro Ala Trp Gln Phe Gly His Gln Arg Gln 
                85                  90                  95      


Cys Trp Gln Asp Gly Gly Arg Lys Asn Cys Ala Lys Asp Pro Val Tyr 
            100                 105                 110         


Arg Lys Met Glu Ser Asp Met His Asn Leu Gln Pro Ser Val Gly Glu 
        115                 120                 125             


Val Asn Gly Asp Arg Gly Asn Phe Met Tyr Ser Gln Trp Asn Gly Gly 
    130                 135                 140                 


Glu Gly Gln Tyr Gly Gln Cys Ala Met Lys Val Asp Phe Lys Glu Lys 
145                 150                 155                 160 


Ala Ala Glu Pro Pro Ala Arg Ala Arg Gly Ala Ile Ala Arg Thr Tyr 
                165                 170                 175     


Phe Tyr Met Arg Asp Gln Tyr Asn Leu Thr Leu Ser Arg Gln Gln Thr 
            180                 185                 190         


Gln Leu Phe Asn Ala Trp Asn Lys Met Tyr Pro Val Thr Asp Trp Glu 
        195                 200                 205             


Cys Glu Arg Asp Glu Arg Ile Ala Lys Val Gln Gly Asn His Asn Pro 
    210                 215                 220                 


Tyr Val Gln Arg Ala Cys Gln Ala Arg Lys Ser 
225                 230                 235 


<210>  2
<211>  784
<212>  PRT
<213>  E. coli

<400>  2

Met Asn Pro Glu Arg Ser Glu Arg Ile Glu Ile Pro Val Leu Pro Leu 
1               5                   10                  15      


Arg Asp Val Val Val Tyr Pro His Met Val Ile Pro Leu Phe Val Gly 
            20                  25                  30          


Arg Glu Lys Ser Ile Arg Cys Leu Glu Ala Ala Met Asp His Asp Lys 
        35                  40                  45              


Lys Ile Met Leu Val Ala Gln Lys Glu Ala Ser Thr Asp Glu Pro Gly 
    50                  55                  60                  


Val Asn Asp Leu Phe Thr Val Gly Thr Val Ala Ser Ile Leu Gln Met 
65                  70                  75                  80  


Leu Lys Leu Pro Asp Gly Thr Val Lys Val Leu Val Glu Gly Leu Gln 
                85                  90                  95      


Arg Ala Arg Ile Ser Ala Leu Ser Asp Asn Gly Glu His Phe Ser Ala 
            100                 105                 110         


Lys Ala Glu Tyr Leu Glu Ser Pro Thr Ile Asp Glu Arg Glu Gln Glu 
        115                 120                 125             


Val Leu Val Arg Thr Ala Ile Ser Gln Phe Glu Gly Tyr Ile Lys Leu 
    130                 135                 140                 


Asn Lys Lys Ile Pro Pro Glu Val Leu Thr Ser Leu Asn Ser Ile Asp 
145                 150                 155                 160 


Asp Pro Ala Arg Leu Ala Asp Thr Ile Ala Ala His Met Pro Leu Lys 
                165                 170                 175     


Leu Ala Asp Lys Gln Ser Val Leu Glu Met Ser Asp Val Asn Glu Arg 
            180                 185                 190         


Leu Glu Tyr Leu Met Ala Met Met Glu Ser Glu Ile Asp Leu Leu Gln 
        195                 200                 205             


Val Glu Lys Arg Ile Arg Asn Arg Val Lys Lys Gln Met Glu Lys Ser 
    210                 215                 220                 


Gln Arg Glu Tyr Tyr Leu Asn Glu Gln Met Lys Ala Ile Gln Lys Glu 
225                 230                 235                 240 


Leu Gly Glu Met Asp Asp Ala Pro Asp Glu Asn Glu Ala Leu Lys Arg 
                245                 250                 255     


Lys Ile Asp Ala Ala Lys Met Pro Lys Glu Ala Lys Glu Lys Ala Glu 
            260                 265                 270         


Ala Glu Leu Gln Lys Leu Lys Met Met Ser Pro Met Ser Ala Glu Ala 
        275                 280                 285             


Thr Val Val Arg Gly Tyr Ile Asp Trp Met Val Gln Val Pro Trp Asn 
    290                 295                 300                 


Ala Arg Ser Lys Val Lys Lys Asp Leu Arg Gln Ala Gln Glu Ile Leu 
305                 310                 315                 320 


Asp Thr Asp His Tyr Gly Leu Glu Arg Val Lys Asp Arg Ile Leu Glu 
                325                 330                 335     


Tyr Leu Ala Val Gln Ser Arg Val Asn Lys Ile Lys Gly Pro Ile Leu 
            340                 345                 350         


Cys Leu Val Gly Pro Pro Gly Val Gly Lys Thr Ser Leu Gly Gln Ser 
        355                 360                 365             


Ile Ala Lys Ala Thr Gly Arg Lys Tyr Val Arg Met Ala Leu Gly Gly 
    370                 375                 380                 


Val Arg Asp Glu Ala Glu Ile Arg Gly His Arg Arg Thr Tyr Ile Gly 
385                 390                 395                 400 


Ser Met Pro Gly Lys Leu Ile Gln Lys Met Ala Lys Val Gly Val Lys 
                405                 410                 415     


Asn Pro Leu Phe Leu Leu Asp Glu Ile Asp Lys Met Ser Ser Asp Met 
            420                 425                 430         


Arg Gly Asp Pro Ala Ser Ala Leu Leu Glu Val Leu Asp Pro Glu Gln 
        435                 440                 445             


Asn Val Ala Phe Ser Asp His Tyr Leu Glu Val Asp Tyr Asp Leu Ser 
    450                 455                 460                 


Asp Val Met Phe Val Ala Thr Ser Asn Ser Met Asn Ile Pro Ala Pro 
465                 470                 475                 480 


Leu Leu Asp Arg Met Glu Val Ile Arg Leu Ser Gly Tyr Thr Glu Asp 
                485                 490                 495     


Glu Lys Leu Asn Ile Ala Lys Arg His Leu Leu Pro Lys Gln Ile Glu 
            500                 505                 510         


Arg Asn Ala Leu Lys Lys Gly Glu Leu Thr Val Asp Asp Ser Ala Ile 
        515                 520                 525             


Ile Gly Ile Ile Arg Tyr Tyr Thr Arg Glu Ala Gly Val Arg Gly Leu 
    530                 535                 540                 


Glu Arg Glu Ile Ser Lys Leu Cys Arg Lys Ala Val Lys Gln Leu Leu 
545                 550                 555                 560 


Leu Asp Lys Ser Leu Lys His Ile Glu Ile Asn Gly Asp Asn Leu His 
                565                 570                 575     


Asp Tyr Leu Gly Val Gln Arg Phe Asp Tyr Gly Arg Ala Asp Asn Glu 
            580                 585                 590         


Asn Arg Val Gly Gln Val Thr Gly Leu Ala Trp Thr Glu Val Gly Gly 
        595                 600                 605             


Asp Leu Leu Thr Ile Glu Thr Ala Cys Val Pro Gly Lys Gly Lys Leu 
    610                 615                 620                 


Thr Tyr Thr Gly Ser Leu Gly Glu Val Met Gln Glu Ser Ile Gln Ala 
625                 630                 635                 640 


Ala Leu Thr Val Val Arg Ala Arg Ala Glu Lys Leu Gly Ile Asn Pro 
                645                 650                 655     


Asp Phe Tyr Glu Lys Arg Asp Ile His Val His Val Pro Glu Gly Ala 
            660                 665                 670         


Thr Pro Lys Asp Gly Pro Ser Ala Gly Ile Ala Met Cys Thr Ala Leu 
        675                 680                 685             


Val Ser Cys Leu Thr Gly Asn Pro Val Arg Ala Asp Val Ala Met Thr 
    690                 695                 700                 


Gly Glu Ile Thr Leu Arg Gly Gln Val Leu Pro Ile Gly Gly Leu Lys 
705                 710                 715                 720 


Glu Lys Leu Leu Ala Ala His Arg Gly Gly Ile Lys Thr Val Leu Ile 
                725                 730                 735     


Pro Phe Glu Asn Lys Arg Asp Leu Glu Glu Ile Pro Asp Asn Val Ile 
            740                 745                 750         


Ala Asp Leu Asp Ile His Pro Val Lys Arg Ile Glu Glu Val Leu Thr 
        755                 760                 765             


Leu Ala Leu Gln Asn Glu Pro Ser Gly Met Gln Val Val Thr Ala Lys 
    770                 775                 780                 


<210>  3
<211>  111
<212>  PRT
<213>  E. coli

<400>  3

Met Val Ser Arg Tyr Val Pro Asp Met Gly Asp Leu Ile Trp Val Asp 
1               5                   10                  15      


Phe Asp Pro Thr Lys Gly Ser Glu Gln Ala Gly His Arg Pro Ala Val 
            20                  25                  30          


Val Leu Ser Pro Phe Met Tyr Asn Asn Lys Thr Gly Met Cys Leu Cys 
        35                  40                  45              


Val Pro Cys Thr Thr Gln Ser Lys Gly Tyr Pro Phe Glu Val Val Leu 
    50                  55                  60                  


Ser Gly Gln Glu Arg Asp Gly Val Ala Leu Ala Asp Gln Val Lys Ser 
65                  70                  75                  80  


Ile Ala Trp Arg Ala Arg Gly Ala Thr Lys Lys Gly Thr Val Ala Pro 
                85                  90                  95      


Glu Glu Leu Gln Leu Ile Lys Ala Lys Ile Asn Val Leu Ile Gly 
            100                 105                 110     


<210>  4
<211>  317
<212>  PRT
<213>  E. coli

<400>  4

Met Arg Ala Lys Leu Leu Gly Ile Val Leu Thr Thr Pro Ile Ala Ile 
1               5                   10                  15      


Ser Ser Phe Ala Ser Thr Glu Thr Leu Ser Phe Thr Pro Asp Asn Ile 
            20                  25                  30          


Asn Ala Asp Ile Ser Leu Gly Thr Leu Ser Gly Lys Thr Lys Glu Arg 
        35                  40                  45              


Val Tyr Leu Ala Glu Glu Gly Gly Arg Lys Val Ser Gln Leu Asp Trp 
    50                  55                  60                  


Lys Phe Asn Asn Ala Ala Ile Ile Lys Gly Ala Ile Asn Trp Asp Leu 
65                  70                  75                  80  


Met Pro Gln Ile Ser Ile Gly Ala Ala Gly Trp Thr Thr Leu Gly Ser 
                85                  90                  95      


Arg Gly Gly Asn Met Val Asp Gln Asp Trp Met Asp Ser Ser Asn Pro 
            100                 105                 110         


Gly Thr Trp Thr Asp Glu Ser Arg His Pro Asp Thr Gln Leu Asn Tyr 
        115                 120                 125             


Ala Asn Glu Phe Asp Leu Asn Ile Lys Gly Trp Leu Leu Asn Glu Pro 
    130                 135                 140                 


Asn Tyr Arg Leu Gly Leu Met Ala Gly Tyr Gln Glu Ser Arg Tyr Ser 
145                 150                 155                 160 


Phe Thr Ala Arg Gly Gly Ser Tyr Ile Tyr Ser Ser Glu Glu Gly Phe 
                165                 170                 175     


Arg Asp Asp Ile Gly Ser Phe Pro Asn Gly Glu Arg Ala Ile Gly Tyr 
            180                 185                 190         


Lys Gln Arg Phe Lys Met Pro Tyr Ile Gly Leu Thr Gly Ser Tyr Arg 
        195                 200                 205             


Tyr Glu Asp Phe Glu Leu Gly Gly Thr Phe Lys Tyr Ser Gly Trp Val 
    210                 215                 220                 


Glu Ser Ser Asp Asn Asp Glu His Tyr Asp Pro Gly Lys Arg Ile Thr 
225                 230                 235                 240 


Tyr Arg Ser Lys Val Lys Asp Gln Asn Tyr Tyr Ser Val Ala Val Asn 
                245                 250                 255     


Ala Gly Tyr Tyr Val Thr Pro Asn Ala Lys Val Tyr Val Glu Gly Ala 
            260                 265                 270         


Trp Asn Arg Val Thr Asn Lys Lys Gly Asn Thr Ser Leu Tyr Asp His 
        275                 280                 285             


Asn Asn Asn Thr Ser Asp Tyr Ser Lys Asn Gly Ala Gly Ile Glu Asn 
    290                 295                 300                 


Tyr Asn Phe Ile Thr Thr Ala Gly Leu Lys Tyr Thr Phe 
305                 310                 315         


<210>  5
<211>  268
<212>  PRT
<213>  E. coli

<400>  5

Met Lys Ala Phe Trp Arg Asn Ala Ala Leu Leu Ala Val Ser Leu Leu 
1               5                   10                  15      


Pro Phe Ser Ser Ala Asn Ala Leu Ala Leu Gln Ala Lys Gln Tyr Gly 
            20                  25                  30          


Asp Phe Asp Arg Tyr Val Leu Ala Leu Ser Trp Gln Thr Gly Phe Cys 
        35                  40                  45              


Gln Ser Gln His Asp Arg Asn Arg Asn Glu Arg Asp Glu Cys Arg Leu 
    50                  55                  60                  


Gln Thr Glu Thr Thr Asn Lys Ala Asp Phe Leu Thr Val His Gly Leu 
65                  70                  75                  80  


Trp Pro Gly Leu Pro Lys Ser Val Ala Ala Arg Gly Val Asp Glu Arg 
                85                  90                  95      


Arg Trp Met Arg Phe Gly Cys Ala Thr Arg Pro Ile Pro Asn Leu Pro 
            100                 105                 110         


Glu Ala Arg Ala Ser Arg Met Cys Ser Ser Pro Glu Thr Gly Leu Ser 
        115                 120                 125             


Leu Glu Thr Ala Ala Lys Leu Ser Glu Val Met Pro Gly Ala Gly Gly 
    130                 135                 140                 


Arg Ser Cys Leu Glu Arg Tyr Glu Tyr Ala Lys His Gly Ala Cys Phe 
145                 150                 155                 160 


Gly Phe Asp Pro Asp Ala Tyr Phe Gly Thr Met Val Arg Leu Asn Gln 
                165                 170                 175     


Glu Ile Lys Glu Ser Glu Ala Gly Lys Phe Leu Ala Asp Asn Tyr Gly 
            180                 185                 190         


Lys Thr Val Ser Arg Arg Asp Phe Asp Ala Ala Phe Ala Lys Ser Trp 
        195                 200                 205             


Gly Lys Glu Asn Val Lys Ala Val Lys Leu Thr Cys Gln Gly Asn Pro 
    210                 215                 220                 


Ala Tyr Leu Thr Glu Ile Gln Ile Ser Ile Lys Ala Asp Ala Ile Asn 
225                 230                 235                 240 


Ala Pro Leu Ser Ala Asn Ser Phe Leu Pro Gln Pro His Pro Gly Asn 
                245                 250                 255     


Cys Gly Lys Thr Phe Val Ile Asp Lys Ala Gly Tyr 
            260                 265             


<210>  6
<211>  644
<212>  PRT
<213>  E. coli

<400>  6

Met Phe Gln Asp Asn Pro Leu Leu Ala Gln Leu Lys Gln Gln Leu His 
1               5                   10                  15      


Ser Gln Thr Pro Arg Ala Glu Gly Val Val Lys Ala Thr Glu Lys Gly 
            20                  25                  30          


Phe Gly Phe Leu Glu Val Asp Ala Gln Lys Ser Tyr Phe Ile Pro Pro 
        35                  40                  45              


Pro Gln Met Lys Lys Val Met His Gly Asp Arg Ile Ile Ala Val Ile 
    50                  55                  60                  


His Ser Glu Lys Glu Arg Glu Ser Ala Glu Pro Glu Glu Leu Val Glu 
65                  70                  75                  80  


Pro Phe Leu Thr Arg Phe Val Gly Lys Val Gln Gly Lys Asn Asp Arg 
                85                  90                  95      


Leu Ala Ile Val Pro Asp His Pro Leu Leu Lys Asp Ala Ile Pro Cys 
            100                 105                 110         


Arg Ala Ala Arg Gly Leu Asn His Glu Phe Lys Glu Gly Asp Trp Ala 
        115                 120                 125             


Val Ala Glu Met Arg Arg His Pro Leu Lys Gly Asp Arg Ser Phe Tyr 
    130                 135                 140                 


Ala Glu Leu Thr Gln Tyr Ile Thr Phe Gly Asp Asp His Phe Val Pro 
145                 150                 155                 160 


Trp Trp Val Thr Leu Ala Arg His Asn Leu Glu Lys Glu Ala Pro Asp 
                165                 170                 175     


Gly Val Ala Thr Glu Met Leu Asp Glu Gly Leu Val Arg Glu Asp Leu 
            180                 185                 190         


Thr Ala Leu Asp Phe Val Thr Ile Asp Ser Ala Ser Thr Glu Asp Met 
        195                 200                 205             


Asp Asp Ala Leu Phe Ala Lys Ala Leu Pro Asp Asp Lys Leu Gln Leu 
    210                 215                 220                 


Ile Val Ala Ile Ala Asp Pro Thr Ala Trp Ile Ala Glu Gly Ser Lys 
225                 230                 235                 240 


Leu Asp Lys Ala Ala Lys Ile Arg Ala Phe Thr Asn Tyr Leu Pro Gly 
                245                 250                 255     


Phe Asn Ile Pro Met Leu Pro Arg Glu Leu Ser Asp Asp Leu Cys Ser 
            260                 265                 270         


Leu Arg Ala Asn Glu Val Arg Pro Val Leu Ala Cys Arg Met Thr Leu 
        275                 280                 285             


Ser Ala Asp Gly Thr Ile Glu Asp Asn Ile Glu Phe Phe Ala Ala Thr 
    290                 295                 300                 


Ile Glu Ser Lys Ala Lys Leu Val Tyr Asp Gln Val Ser Asp Trp Leu 
305                 310                 315                 320 


Glu Asn Thr Gly Asp Trp Gln Pro Glu Ser Glu Ala Ile Ala Glu Gln 
                325                 330                 335     


Val Arg Leu Leu Ala Gln Ile Cys Gln Arg Arg Gly Glu Trp Arg His 
            340                 345                 350         


Asn His Ala Leu Val Phe Lys Asp Arg Pro Asp Tyr Arg Phe Ile Leu 
        355                 360                 365             


Gly Glu Lys Gly Glu Val Leu Asp Ile Val Ala Glu Pro Arg Arg Ile 
    370                 375                 380                 


Ala Asn Arg Ile Val Glu Glu Ala Met Ile Ala Ala Asn Ile Cys Ala 
385                 390                 395                 400 


Ala Arg Val Leu Arg Asp Lys Leu Gly Phe Gly Ile Tyr Asn Val His 
                405                 410                 415     


Met Gly Phe Asp Pro Ala Asn Ala Asp Ala Leu Ala Ala Leu Leu Lys 
            420                 425                 430         


Thr His Gly Leu His Val Asp Ala Glu Glu Val Leu Thr Leu Asp Gly 
        435                 440                 445             


Phe Cys Lys Leu Arg Arg Glu Leu Asp Ala Gln Pro Thr Gly Phe Leu 
    450                 455                 460                 


Asp Ser Arg Ile Arg Arg Phe Gln Ser Phe Ala Glu Ile Ser Thr Glu 
465                 470                 475                 480 


Pro Gly Pro His Phe Gly Leu Gly Leu Glu Ala Tyr Ala Thr Trp Thr 
                485                 490                 495     


Ser Pro Ile Arg Lys Tyr Gly Asp Met Ile Asn His Arg Leu Leu Lys 
            500                 505                 510         


Ala Val Ile Lys Gly Glu Thr Ala Thr Arg Pro Gln Asp Glu Ile Thr 
        515                 520                 525             


Val Gln Met Ala Glu Arg Arg Arg Leu Asn Arg Met Ala Glu Arg Asp 
    530                 535                 540                 


Val Gly Asp Trp Leu Tyr Ala Arg Phe Leu Lys Asp Lys Ala Gly Thr 
545                 550                 555                 560 


Asp Thr Arg Phe Ala Ala Glu Ile Val Asp Ile Ser Arg Gly Gly Met 
                565                 570                 575     


Arg Val Arg Leu Val Asp Asn Gly Ala Ile Ala Phe Ile Pro Ala Pro 
            580                 585                 590         


Phe Leu His Ala Val Arg Asp Glu Leu Val Cys Ser Gln Glu Asn Gly 
        595                 600                 605             


Thr Val Gln Ile Lys Gly Glu Thr Val Tyr Lys Val Thr Asp Val Ile 
    610                 615                 620                 


Asp Val Thr Ile Ala Glu Val Arg Met Glu Thr Arg Ser Ile Ile Ala 
625                 630                 635                 640 


Arg Pro Val Ala 
                


<210>  7
<211>  502
<212>  PRT
<213>  E. coli

<400>  7

Met Thr Glu Lys Lys Tyr Ile Val Ala Leu Asp Gln Gly Thr Thr Ser 
1               5                   10                  15      


Ser Arg Ala Val Val Met Asp His Asp Ala Asn Ile Ile Ser Val Ser 
            20                  25                  30          


Gln Arg Glu Phe Glu Gln Ile Tyr Pro Lys Pro Gly Trp Val Glu His 
        35                  40                  45              


Asp Pro Met Glu Ile Trp Ala Thr Gln Ser Ser Thr Leu Val Glu Val 
    50                  55                  60                  


Leu Ala Lys Ala Asp Ile Ser Ser Asp Gln Ile Ala Ala Ile Gly Ile 
65                  70                  75                  80  


Thr Asn Gln Arg Glu Thr Thr Ile Val Trp Glu Lys Glu Thr Gly Lys 
                85                  90                  95      


Pro Ile Tyr Asn Ala Ile Val Trp Gln Cys Arg Arg Thr Ala Glu Ile 
            100                 105                 110         


Cys Glu His Leu Lys Arg Asp Gly Leu Glu Asp Tyr Ile Arg Ser Asn 
        115                 120                 125             


Thr Gly Leu Val Ile Asp Pro Tyr Phe Ser Gly Thr Lys Val Lys Trp 
    130                 135                 140                 


Ile Leu Asp His Val Glu Gly Ser Arg Glu Arg Ala Arg Arg Gly Glu 
145                 150                 155                 160 


Leu Leu Phe Gly Thr Val Asp Thr Trp Leu Ile Trp Lys Met Thr Gln 
                165                 170                 175     


Gly Arg Val His Val Thr Asp Tyr Thr Asn Ala Ser Arg Thr Met Leu 
            180                 185                 190         


Phe Asn Ile His Thr Leu Asp Trp Asp Asp Lys Met Leu Glu Val Leu 
        195                 200                 205             


Asp Ile Pro Arg Glu Met Leu Pro Glu Val Arg Arg Ser Ser Glu Val 
    210                 215                 220                 


Tyr Gly Gln Thr Asn Ile Gly Gly Lys Gly Gly Thr Arg Ile Pro Ile 
225                 230                 235                 240 


Ser Gly Ile Ala Gly Asp Gln Gln Ala Ala Leu Phe Gly Gln Leu Cys 
                245                 250                 255     


Val Lys Glu Gly Met Ala Lys Asn Thr Tyr Gly Thr Gly Cys Phe Met 
            260                 265                 270         


Leu Met Asn Thr Gly Glu Lys Ala Val Lys Ser Glu Asn Gly Leu Leu 
        275                 280                 285             


Thr Thr Ile Ala Cys Gly Pro Thr Gly Glu Val Asn Tyr Ala Leu Glu 
    290                 295                 300                 


Gly Ala Val Phe Met Ala Gly Ala Ser Ile Gln Trp Leu Arg Asp Glu 
305                 310                 315                 320 


Met Lys Leu Ile Asn Asp Ala Tyr Asp Ser Glu Tyr Phe Ala Thr Lys 
                325                 330                 335     


Val Gln Asn Thr Asn Gly Val Tyr Val Val Pro Ala Phe Thr Gly Leu 
            340                 345                 350         


Gly Ala Pro Tyr Trp Asp Pro Tyr Ala Arg Gly Ala Ile Phe Gly Leu 
        355                 360                 365             


Thr Arg Gly Val Asn Ala Asn His Ile Ile Arg Ala Thr Leu Glu Ser 
    370                 375                 380                 


Ile Ala Tyr Gln Thr Arg Asp Val Leu Glu Ala Met Gln Ala Asp Ser 
385                 390                 395                 400 


Gly Ile Arg Leu His Ala Leu Arg Val Asp Gly Gly Ala Val Ala Asn 
                405                 410                 415     


Asn Phe Leu Met Gln Phe Gln Ser Asp Ile Leu Gly Thr Arg Val Glu 
            420                 425                 430         


Arg Pro Glu Val Arg Glu Val Thr Ala Leu Gly Ala Ala Tyr Leu Ala 
        435                 440                 445             


Gly Leu Ala Val Gly Phe Trp Gln Asn Leu Asp Glu Leu Gln Glu Lys 
    450                 455                 460                 


Ala Val Ile Glu Arg Glu Phe Arg Pro Gly Ile Glu Thr Thr Glu Arg 
465                 470                 475                 480 


Asn Tyr Arg Tyr Ala Gly Trp Lys Lys Ala Val Lys Arg Ala Met Ala 
                485                 490                 495     


Trp Glu Glu His Asp Glu 
            500         


<210>  8
<211>  450
<212>  PRT
<213>  E. coli

<400>  8

Met Thr Lys His Tyr Asp Tyr Ile Ala Ile Gly Gly Gly Ser Gly Gly 
1               5                   10                  15      


Ile Ala Ser Ile Asn Arg Ala Ala Met Tyr Gly Gln Lys Cys Ala Leu 
            20                  25                  30          


Ile Glu Ala Lys Glu Leu Gly Gly Thr Cys Val Asn Val Gly Cys Val 
        35                  40                  45              


Pro Lys Lys Val Met Trp His Ala Ala Gln Ile Arg Glu Ala Ile His 
    50                  55                  60                  


Met Tyr Gly Pro Asp Tyr Gly Phe Asp Thr Thr Ile Asn Lys Phe Asn 
65                  70                  75                  80  


Trp Glu Thr Leu Ile Ala Ser Arg Thr Ala Tyr Ile Asp Arg Ile His 
                85                  90                  95      


Thr Ser Tyr Glu Asn Val Leu Gly Lys Asn Asn Val Asp Val Ile Lys 
            100                 105                 110         


Gly Phe Ala Arg Phe Val Asp Ala Lys Thr Leu Glu Val Asn Gly Glu 
        115                 120                 125             


Thr Ile Thr Ala Asp His Ile Leu Ile Ala Thr Gly Gly Arg Pro Ser 
    130                 135                 140                 


His Pro Asp Ile Pro Gly Val Glu Tyr Gly Ile Asp Ser Asp Gly Phe 
145                 150                 155                 160 


Phe Ala Leu Pro Ala Leu Pro Glu Arg Val Ala Val Val Gly Ala Gly 
                165                 170                 175     


Tyr Ile Ala Val Glu Leu Ala Gly Val Ile Asn Gly Leu Gly Ala Lys 
            180                 185                 190         


Thr His Leu Phe Val Arg Lys His Ala Pro Leu Arg Ser Phe Asp Pro 
        195                 200                 205             


Met Ile Ser Glu Thr Leu Val Glu Val Met Asn Ala Glu Gly Pro Gln 
    210                 215                 220                 


Leu His Thr Asn Ala Ile Pro Lys Ala Val Val Lys Asn Thr Asp Gly 
225                 230                 235                 240 


Ser Leu Thr Leu Glu Leu Glu Asp Gly Arg Ser Glu Thr Val Asp Cys 
                245                 250                 255     


Leu Ile Trp Ala Ile Gly Arg Glu Pro Ala Asn Asp Asn Ile Asn Leu 
            260                 265                 270         


Glu Ala Ala Gly Val Lys Thr Asn Glu Lys Gly Tyr Ile Val Val Asp 
        275                 280                 285             


Lys Tyr Gln Asn Thr Asn Ile Glu Gly Ile Tyr Ala Val Gly Asp Asn 
    290                 295                 300                 


Thr Gly Ala Val Glu Leu Thr Pro Val Ala Val Ala Ala Gly Arg Arg 
305                 310                 315                 320 


Leu Ser Glu Arg Leu Phe Asn Asn Lys Pro Asp Glu His Leu Asp Tyr 
                325                 330                 335     


Ser Asn Ile Pro Thr Val Val Phe Ser His Pro Pro Ile Gly Thr Val 
            340                 345                 350         


Gly Leu Thr Glu Pro Gln Ala Arg Glu Gln Tyr Gly Asp Asp Gln Val 
        355                 360                 365             


Lys Val Tyr Lys Ser Ser Phe Thr Ala Met Tyr Thr Ala Val Thr Thr 
    370                 375                 380                 


His Arg Gln Pro Cys Arg Met Lys Leu Val Cys Val Gly Ser Glu Glu 
385                 390                 395                 400 


Lys Ile Val Gly Ile His Gly Ile Gly Phe Gly Met Asp Glu Met Leu 
                405                 410                 415     


Gln Gly Phe Ala Val Ala Leu Lys Met Gly Ala Thr Lys Lys Asp Phe 
            420                 425                 430         


Asp Asn Thr Val Ala Ile His Pro Thr Ala Ala Glu Glu Phe Val Thr 
        435                 440                 445             


Met Arg 
    450 


<210>  9
<211>  518
<212>  PRT
<213>  E. coli

<400>  9

Met Ile Pro Asp Val Ser Gln Ala Leu Ala Trp Leu Glu Lys His Pro 
1               5                   10                  15      


Gln Ala Leu Lys Gly Ile Gln Arg Gly Leu Glu Arg Glu Thr Leu Arg 
            20                  25                  30          


Val Asn Ala Asp Gly Thr Leu Ala Thr Thr Gly His Pro Glu Ala Leu 
        35                  40                  45              


Gly Ser Ala Leu Thr His Lys Trp Ile Thr Thr Asp Phe Ala Glu Ala 
    50                  55                  60                  


Leu Leu Glu Phe Ile Thr Pro Val Asp Gly Asp Ile Glu His Met Leu 
65                  70                  75                  80  


Thr Phe Met Arg Asp Leu His Arg Tyr Thr Ala Arg Asn Met Gly Asp 
                85                  90                  95      


Glu Arg Met Trp Pro Leu Ser Met Pro Cys Tyr Ile Ala Glu Gly Gln 
            100                 105                 110         


Asp Ile Glu Leu Ala Gln Tyr Gly Thr Ser Asn Thr Gly Arg Phe Lys 
        115                 120                 125             


Thr Leu Tyr Arg Glu Gly Leu Lys Asn Arg Tyr Gly Ala Leu Met Gln 
    130                 135                 140                 


Thr Ile Ser Gly Val His Tyr Asn Phe Ser Leu Pro Met Ala Phe Trp 
145                 150                 155                 160 


Gln Ala Lys Cys Gly Asp Ile Ser Gly Ala Asp Ala Lys Glu Lys Ile 
                165                 170                 175     


Ser Ala Gly Tyr Phe Arg Val Ile Arg Asn Tyr Tyr Arg Phe Gly Trp 
            180                 185                 190         


Val Ile Pro Tyr Leu Phe Gly Ala Ser Pro Ala Ile Cys Ser Ser Phe 
        195                 200                 205             


Leu Gln Gly Lys Pro Thr Ser Leu Pro Phe Glu Lys Thr Glu Cys Gly 
    210                 215                 220                 


Met Tyr Tyr Leu Pro Tyr Ala Thr Ser Leu Arg Leu Ser Asp Leu Gly 
225                 230                 235                 240 


Tyr Thr Asn Lys Ser Gln Ser Asn Leu Gly Ile Thr Phe Asn Asp Leu 
                245                 250                 255     


Tyr Glu Tyr Val Ala Gly Leu Lys Gln Ala Ile Lys Thr Pro Ser Glu 
            260                 265                 270         


Glu Tyr Ala Lys Ile Gly Ile Glu Lys Asp Gly Lys Arg Leu Gln Ile 
        275                 280                 285             


Asn Ser Asn Val Leu Gln Ile Glu Asn Glu Leu Tyr Ala Pro Ile Arg 
    290                 295                 300                 


Pro Lys Arg Val Thr Arg Ser Gly Glu Ser Pro Ser Asp Ala Leu Leu 
305                 310                 315                 320 


Arg Gly Gly Ile Glu Tyr Ile Glu Val Arg Ser Leu Asp Ile Asn Pro 
                325                 330                 335     


Phe Ser Pro Ile Gly Val Asp Glu Gln Gln Val Arg Phe Leu Asp Leu 
            340                 345                 350         


Phe Met Val Trp Cys Ala Leu Ala Asp Ala Pro Glu Met Ser Ser Ser 
        355                 360                 365             


Glu Leu Ala Cys Thr Arg Val Asn Trp Asn Arg Val Ile Leu Glu Gly 
    370                 375                 380                 


Arg Lys Pro Gly Leu Thr Leu Gly Ile Gly Cys Glu Thr Ala Gln Phe 
385                 390                 395                 400 


Pro Leu Pro Gln Val Gly Lys Asp Leu Phe Arg Asp Leu Lys Arg Val 
                405                 410                 415     


Ala Gln Thr Leu Asp Ser Ile Asn Gly Gly Glu Ala Tyr Gln Lys Val 
            420                 425                 430         


Cys Asp Glu Leu Val Ala Cys Phe Asp Asn Pro Asp Leu Thr Phe Ser 
        435                 440                 445             


Ala Arg Ile Leu Arg Ser Met Ile Asp Thr Gly Ile Gly Gly Thr Gly 
    450                 455                 460                 


Lys Ala Phe Ala Glu Ala Tyr Arg Asn Leu Leu Arg Glu Glu Pro Leu 
465                 470                 475                 480 


Glu Ile Leu Arg Glu Glu Asp Phe Val Ala Glu Arg Glu Ala Ser Glu 
                485                 490                 495     


Arg Arg Gln Gln Glu Met Glu Ala Ala Asp Thr Glu Pro Phe Ala Val 
            500                 505                 510         


Trp Leu Glu Lys His Ala 
        515             


<210>  10
<211>  471
<212>  PRT
<213>  E. coli

<400>  10

Met Glu Asn Phe Lys His Leu Pro Glu Pro Phe Arg Ile Arg Val Ile 
1               5                   10                  15      


Glu Pro Val Lys Arg Thr Thr Arg Ala Tyr Arg Glu Glu Ala Ile Ile 
            20                  25                  30          


Lys Ser Gly Met Asn Pro Phe Leu Leu Asp Ser Glu Asp Val Phe Ile 
        35                  40                  45              


Asp Leu Leu Thr Asp Ser Gly Thr Gly Ala Val Thr Gln Ser Met Gln 
    50                  55                  60                  


Ala Ala Met Met Arg Gly Asp Glu Ala Tyr Ser Gly Ser Arg Ser Tyr 
65                  70                  75                  80  


Tyr Ala Leu Ala Glu Ser Val Lys Asn Ile Phe Gly Tyr Gln Tyr Thr 
                85                  90                  95      


Ile Pro Thr His Gln Gly Arg Gly Ala Glu Gln Ile Tyr Ile Pro Val 
            100                 105                 110         


Leu Ile Lys Lys Arg Glu Gln Glu Lys Gly Leu Asp Arg Ser Lys Met 
        115                 120                 125             


Val Ala Phe Ser Asn Tyr Phe Phe Asp Thr Thr Gln Gly His Ser Gln 
    130                 135                 140                 


Ile Asn Gly Cys Thr Val Arg Asn Val Tyr Ile Lys Glu Ala Phe Asp 
145                 150                 155                 160 


Thr Gly Val Arg Tyr Asp Phe Lys Gly Asn Phe Asp Leu Glu Gly Leu 
                165                 170                 175     


Glu Arg Gly Ile Glu Glu Val Gly Pro Asn Asn Val Pro Tyr Ile Val 
            180                 185                 190         


Ala Thr Ile Thr Ser Asn Ser Ala Gly Gly Gln Pro Val Ser Leu Ala 
        195                 200                 205             


Asn Leu Lys Ala Met Tyr Ser Ile Ala Lys Lys Tyr Asp Ile Pro Val 
    210                 215                 220                 


Val Met Asp Ser Ala Arg Phe Ala Glu Asn Ala Tyr Phe Ile Lys Gln 
225                 230                 235                 240 


Arg Glu Ala Glu Tyr Lys Asp Trp Thr Ile Glu Gln Ile Thr Arg Glu 
                245                 250                 255     


Thr Tyr Lys Tyr Ala Asp Met Leu Ala Met Ser Ala Lys Lys Asp Ala 
            260                 265                 270         


Met Val Pro Met Gly Gly Leu Leu Cys Met Lys Asp Asp Ser Phe Phe 
        275                 280                 285             


Asp Val Tyr Thr Glu Cys Arg Thr Leu Cys Val Val Gln Glu Gly Phe 
    290                 295                 300                 


Pro Thr Tyr Gly Gly Leu Glu Gly Gly Ala Met Glu Arg Leu Ala Val 
305                 310                 315                 320 


Gly Leu Tyr Asp Gly Met Asn Leu Asp Trp Leu Ala Tyr Arg Ile Ala 
                325                 330                 335     


Gln Val Gln Tyr Leu Val Asp Gly Leu Glu Glu Ile Gly Val Val Cys 
            340                 345                 350         


Gln Gln Ala Gly Gly His Ala Ala Phe Val Asp Ala Gly Lys Leu Leu 
        355                 360                 365             


Pro His Ile Pro Ala Asp Gln Phe Pro Ala Gln Ala Leu Ala Cys Glu 
    370                 375                 380                 


Leu Tyr Lys Val Ala Gly Ile Arg Ala Val Glu Ile Gly Ser Phe Leu 
385                 390                 395                 400 


Leu Gly Arg Asp Pro Lys Thr Gly Lys Gln Leu Pro Cys Pro Ala Glu 
                405                 410                 415     


Leu Leu Arg Leu Thr Ile Pro Arg Ala Thr Tyr Thr Gln Thr His Met 
            420                 425                 430         


Asp Phe Ile Ile Glu Ala Phe Lys His Val Lys Glu Asn Ala Ala Asn 
        435                 440                 445             


Ile Lys Gly Leu Thr Phe Thr Tyr Glu Pro Lys Val Leu Arg His Phe 
    450                 455                 460                 


Thr Ala Lys Leu Lys Glu Val 
465                 470     


<210>  11
<211>  1061
<212>  PRT
<213>  E. coli

<400>  11

Met Lys Arg Met Leu Ile Asn Ala Thr Gln Gln Glu Glu Leu Arg Val 
1               5                   10                  15      


Ala Leu Val Asp Gly Gln Arg Leu Tyr Asp Leu Asp Ile Glu Ser Pro 
            20                  25                  30          


Gly His Glu Gln Lys Lys Ala Asn Ile Tyr Lys Gly Lys Ile Thr Arg 
        35                  40                  45              


Ile Glu Pro Ser Leu Glu Ala Ala Phe Val Asp Tyr Gly Ala Glu Arg 
    50                  55                  60                  


His Gly Phe Leu Pro Leu Lys Glu Ile Ala Arg Glu Tyr Phe Pro Ala 
65                  70                  75                  80  


Asn Tyr Ser Ala His Gly Arg Pro Asn Ile Lys Asp Val Leu Arg Glu 
                85                  90                  95      


Gly Gln Glu Val Ile Val Gln Ile Asp Lys Glu Glu Arg Gly Asn Lys 
            100                 105                 110         


Gly Ala Ala Leu Thr Thr Phe Ile Ser Leu Ala Gly Ser Tyr Leu Val 
        115                 120                 125             


Leu Met Pro Asn Asn Pro Arg Ala Gly Gly Ile Ser Arg Arg Ile Glu 
    130                 135                 140                 


Gly Asp Asp Arg Thr Glu Leu Lys Glu Ala Leu Ala Ser Leu Glu Leu 
145                 150                 155                 160 


Pro Glu Gly Met Gly Leu Ile Val Arg Thr Ala Gly Val Gly Lys Ser 
                165                 170                 175     


Ala Glu Ala Leu Gln Trp Asp Leu Ser Phe Arg Leu Lys His Trp Glu 
            180                 185                 190         


Ala Ile Lys Lys Ala Ala Glu Ser Arg Pro Ala Pro Phe Leu Ile His 
        195                 200                 205             


Gln Glu Ser Asn Val Ile Val Arg Ala Phe Arg Asp Tyr Leu Arg Gln 
    210                 215                 220                 


Asp Ile Gly Glu Ile Leu Ile Asp Asn Pro Lys Val Leu Glu Leu Ala 
225                 230                 235                 240 


Arg Gln His Ile Ala Ala Leu Gly Arg Pro Asp Phe Ser Ser Lys Ile 
                245                 250                 255     


Lys Leu Tyr Thr Gly Glu Ile Pro Leu Phe Ser His Tyr Gln Ile Glu 
            260                 265                 270         


Ser Gln Ile Glu Ser Ala Phe Gln Arg Glu Val Arg Leu Pro Ser Gly 
        275                 280                 285             


Gly Ser Ile Val Ile Asp Ser Thr Glu Ala Leu Thr Ala Ile Asp Ile 
    290                 295                 300                 


Asn Ser Ala Arg Ala Thr Arg Gly Gly Asp Ile Glu Glu Thr Ala Phe 
305                 310                 315                 320 


Asn Thr Asn Leu Glu Ala Ala Asp Glu Ile Ala Arg Gln Leu Arg Leu 
                325                 330                 335     


Arg Asp Leu Gly Gly Leu Ile Val Ile Asp Phe Ile Asp Met Thr Pro 
            340                 345                 350         


Val Arg His Gln Arg Ala Val Glu Asn Arg Leu Arg Glu Ala Val Arg 
        355                 360                 365             


Gln Asp Arg Ala Arg Ile Gln Ile Ser His Ile Ser Arg Phe Gly Leu 
    370                 375                 380                 


Leu Glu Met Ser Arg Gln Arg Leu Ser Pro Ser Leu Gly Glu Ser Ser 
385                 390                 395                 400 


His His Val Cys Pro Arg Cys Ser Gly Thr Gly Thr Val Arg Asp Asn 
                405                 410                 415     


Glu Ser Leu Ser Leu Ser Ile Leu Arg Leu Ile Glu Glu Glu Ala Leu 
            420                 425                 430         


Lys Glu Asn Thr Gln Glu Val His Ala Ile Val Pro Val Pro Ile Ala 
        435                 440                 445             


Ser Tyr Leu Leu Asn Glu Lys Arg Ser Ala Val Asn Ala Ile Glu Thr 
    450                 455                 460                 


Arg Gln Asp Gly Val Arg Cys Val Ile Val Pro Asn Asp Gln Met Glu 
465                 470                 475                 480 


Thr Pro His Tyr His Val Leu Arg Val Arg Lys Gly Glu Glu Thr Pro 
                485                 490                 495     


Thr Leu Ser Tyr Met Leu Pro Lys Leu His Glu Glu Ala Met Ala Leu 
            500                 505                 510         


Pro Ser Glu Glu Glu Phe Ala Glu Arg Lys Arg Pro Glu Gln Pro Ala 
        515                 520                 525             


Leu Ala Thr Phe Ala Met Pro Asp Val Pro Pro Ala Pro Thr Pro Ala 
    530                 535                 540                 


Glu Pro Ala Ala Pro Val Val Ala Pro Ala Pro Lys Ala Ala Pro Ala 
545                 550                 555                 560 


Thr Pro Ala Ala Pro Ala Gln Pro Gly Leu Leu Ser Arg Phe Phe Gly 
                565                 570                 575     


Ala Leu Lys Ala Leu Phe Ser Gly Gly Glu Glu Thr Lys Pro Thr Glu 
            580                 585                 590         


Gln Pro Ala Pro Lys Ala Glu Ala Lys Pro Glu Arg Gln Gln Asp Arg 
        595                 600                 605             


Arg Lys Pro Arg Gln Asn Asn Arg Arg Asp Arg Asn Glu Arg Arg Asp 
    610                 615                 620                 


Thr Arg Ser Glu Arg Thr Glu Gly Ser Asp Asn Arg Glu Glu Asn Arg 
625                 630                 635                 640 


Arg Asn Arg Arg Gln Ala Gln Gln Gln Thr Ala Glu Thr Arg Glu Ser 
                645                 650                 655     


Arg Gln Gln Ala Glu Val Thr Glu Lys Ala Arg Thr Ala Asp Glu Gln 
            660                 665                 670         


Gln Ala Pro Arg Arg Glu Arg Ser Arg Arg Arg Asn Asp Asp Lys Arg 
        675                 680                 685             


Gln Ala Gln Gln Glu Ala Lys Ala Leu Asn Val Glu Glu Gln Ser Val 
    690                 695                 700                 


Gln Glu Thr Glu Gln Glu Glu Arg Val Arg Pro Val Gln Pro Arg Arg 
705                 710                 715                 720 


Lys Gln Arg Gln Leu Asn Gln Lys Val Arg Tyr Glu Gln Ser Val Ala 
                725                 730                 735     


Glu Glu Ala Val Val Ala Pro Val Val Glu Glu Thr Val Ala Ala Glu 
            740                 745                 750         


Pro Ile Val Gln Glu Ala Pro Ala Pro Arg Thr Glu Leu Val Lys Val 
        755                 760                 765             


Pro Leu Pro Val Val Ala Gln Thr Ala Pro Glu Gln Gln Glu Glu Asn 
    770                 775                 780                 


Asn Ala Asp Asn Arg Asp Asn Gly Gly Met Pro Arg Arg Ser Arg Arg 
785                 790                 795                 800 


Ser Pro Arg His Leu Arg Val Ser Gly Gln Arg Arg Arg Arg Tyr Arg 
                805                 810                 815     


Asp Glu Arg Tyr Pro Thr Gln Ser Pro Met Pro Leu Thr Val Ala Cys 
            820                 825                 830         


Ala Ser Pro Glu Leu Ala Ser Gly Lys Val Trp Ile Arg Tyr Pro Ile 
        835                 840                 845             


Val Arg Pro Gln Asp Val Gln Val Glu Glu Gln Arg Glu Gln Glu Glu 
    850                 855                 860                 


Val His Val Gln Pro Met Val Thr Glu Val Pro Val Ala Ala Ala Ile 
865                 870                 875                 880 


Glu Pro Val Val Ser Ala Pro Val Val Glu Glu Val Ala Gly Val Val 
                885                 890                 895     


Glu Ala Pro Val Gln Val Ala Glu Pro Gln Pro Glu Val Val Glu Thr 
            900                 905                 910         


Thr His Pro Glu Val Ile Ala Ala Ala Val Thr Glu Gln Pro Gln Val 
        915                 920                 925             


Ile Thr Glu Ser Asp Val Ala Val Ala Gln Glu Val Ala Glu Gln Ala 
    930                 935                 940                 


Glu Pro Val Val Glu Pro Gln Glu Glu Thr Ala Asp Ile Glu Glu Val 
945                 950                 955                 960 


Val Glu Thr Ala Glu Val Val Val Ala Glu Pro Glu Val Val Ala Gln 
                965                 970                 975     


Pro Ala Ala Pro Val Val Ala Glu Val Ala Ala Glu Val Glu Thr Val 
            980                 985                 990         


Ala Ala Val Glu Pro Glu Val Thr  Val Glu His Asn His  Ala Thr Ala 
        995                 1000                 1005             


Pro Met  Thr Arg Ala Pro Ala  Pro Glu Tyr Val Pro  Glu Ala Pro 
    1010                 1015                 1020             


Arg His  Ser Asp Trp Gln Arg  Pro Thr Phe Ala Phe  Glu Gly Lys 
    1025                 1030                 1035             


Gly Ala  Ala Gly Gly His Thr  Ala Thr His His Ala  Ser Ala Ala 
    1040                 1045                 1050             


Pro Ala  Arg Pro Gln Pro Val  Glu 
    1055                 1060     


<210>  12
<211>  447
<212>  PRT
<213>  E. coli

<400>  12

Met Asp Gln Thr Tyr Ser Leu Glu Ser Phe Leu Asn His Val Gln Lys 
1               5                   10                  15      


Arg Asp Pro Asn Gln Thr Glu Phe Ala Gln Ala Val Arg Glu Val Met 
            20                  25                  30          


Thr Thr Leu Trp Pro Phe Leu Glu Gln Asn Pro Lys Tyr Arg Gln Met 
        35                  40                  45              


Ser Leu Leu Glu Arg Leu Val Glu Pro Glu Arg Val Ile Gln Phe Arg 
    50                  55                  60                  


Val Val Trp Val Asp Asp Arg Asn Gln Ile Gln Val Asn Arg Ala Trp 
65                  70                  75                  80  


Arg Val Gln Phe Ser Ser Ala Ile Gly Pro Tyr Lys Gly Gly Met Arg 
                85                  90                  95      


Phe His Pro Ser Val Asn Leu Ser Ile Leu Lys Phe Leu Gly Phe Glu 
            100                 105                 110         


Gln Thr Phe Lys Asn Ala Leu Thr Thr Leu Pro Met Gly Gly Gly Lys 
        115                 120                 125             


Gly Gly Ser Asp Phe Asp Pro Lys Gly Lys Ser Glu Gly Glu Val Met 
    130                 135                 140                 


Arg Phe Cys Gln Ala Leu Met Thr Glu Leu Tyr Arg His Leu Gly Ala 
145                 150                 155                 160 


Asp Thr Asp Val Pro Ala Gly Asp Ile Gly Val Gly Gly Arg Glu Val 
                165                 170                 175     


Gly Phe Met Ala Gly Met Met Lys Lys Leu Ser Asn Asn Thr Ala Cys 
            180                 185                 190         


Val Phe Thr Gly Lys Gly Leu Ser Phe Gly Gly Ser Leu Ile Arg Pro 
        195                 200                 205             


Glu Ala Thr Gly Tyr Gly Leu Val Tyr Phe Thr Glu Ala Met Leu Lys 
    210                 215                 220                 


Arg His Gly Met Gly Phe Glu Gly Met Arg Val Ser Val Ser Gly Ser 
225                 230                 235                 240 


Gly Asn Val Ala Gln Tyr Ala Ile Glu Lys Ala Met Glu Phe Gly Ala 
                245                 250                 255     


Arg Val Ile Thr Ala Ser Asp Ser Ser Gly Thr Val Val Asp Glu Ser 
            260                 265                 270         


Gly Phe Thr Lys Glu Lys Leu Ala Arg Leu Ile Glu Ile Lys Ala Ser 
        275                 280                 285             


Arg Asp Gly Arg Val Ala Asp Tyr Ala Lys Glu Phe Gly Leu Val Tyr 
    290                 295                 300                 


Leu Glu Gly Gln Gln Pro Trp Ser Leu Pro Val Asp Ile Ala Leu Pro 
305                 310                 315                 320 


Cys Ala Thr Gln Asn Glu Leu Asp Val Asp Ala Ala His Gln Leu Ile 
                325                 330                 335     


Ala Asn Gly Val Lys Ala Val Ala Glu Gly Ala Asn Met Pro Thr Thr 
            340                 345                 350         


Ile Glu Ala Thr Glu Leu Phe Gln Gln Ala Gly Val Leu Phe Ala Pro 
        355                 360                 365             


Gly Lys Ala Ala Asn Ala Gly Gly Val Ala Thr Ser Gly Leu Glu Met 
    370                 375                 380                 


Ala Gln Asn Ala Ala Arg Leu Gly Trp Lys Ala Glu Lys Val Asp Ala 
385                 390                 395                 400 


Arg Leu His His Ile Met Leu Asp Ile His His Ala Cys Val Glu His 
                405                 410                 415     


Gly Gly Glu Gly Glu Gln Thr Asn Tyr Val Gln Gly Ala Asn Ile Ala 
            420                 425                 430         


Gly Phe Val Lys Val Ala Asp Ala Met Leu Ala Gln Gly Val Ile 
        435                 440                 445         


<210>  13
<211>  454
<212>  PRT
<213>  E. coli

<400>  13

Met Ile Ser Leu Phe Asp Met Phe Lys Val Gly Ile Gly Pro Ser Ser 
1               5                   10                  15      


Ser His Thr Val Gly Pro Met Lys Ala Gly Lys Gln Phe Val Asp Asp 
            20                  25                  30          


Leu Val Glu Lys Gly Leu Leu Asp Ser Val Thr Arg Val Ala Val Asp 
        35                  40                  45              


Val Tyr Gly Ser Leu Ser Leu Thr Gly Lys Gly His His Thr Asp Ile 
    50                  55                  60                  


Ala Ile Ile Met Gly Leu Ala Gly Asn Glu Pro Ala Thr Val Asp Ile 
65                  70                  75                  80  


Asp Ser Ile Pro Gly Phe Ile Arg Asp Val Glu Glu Arg Glu Arg Leu 
                85                  90                  95      


Leu Leu Ala Gln Gly Arg His Glu Val Asp Phe Pro Arg Asp Asn Gly 
            100                 105                 110         


Met Arg Phe His Asn Gly Asn Leu Pro Leu His Glu Asn Gly Met Gln 
        115                 120                 125             


Ile His Ala Tyr Asn Gly Asp Glu Val Val Tyr Ser Lys Thr Tyr Tyr 
    130                 135                 140                 


Ser Ile Gly Gly Gly Phe Ile Val Asp Glu Glu His Phe Gly Gln Asp 
145                 150                 155                 160 


Ala Ala Asn Glu Val Ser Val Pro Tyr Pro Phe Lys Ser Ala Thr Glu 
                165                 170                 175     


Leu Leu Ala Tyr Cys Asn Glu Thr Gly Tyr Ser Leu Ser Gly Leu Ala 
            180                 185                 190         


Met Gln Asn Glu Leu Ala Leu His Ser Lys Lys Glu Ile Asp Glu Tyr 
        195                 200                 205             


Phe Ala His Val Trp Gln Thr Met Gln Ala Cys Ile Asp Arg Gly Met 
    210                 215                 220                 


Asn Thr Glu Gly Val Leu Pro Gly Pro Leu Arg Val Pro Arg Arg Ala 
225                 230                 235                 240 


Ser Ala Leu Arg Arg Met Leu Val Ser Ser Asp Lys Leu Ser Asn Asp 
                245                 250                 255     


Pro Met Asn Val Ile Asp Trp Val Asn Met Phe Ala Leu Ala Val Asn 
            260                 265                 270         


Glu Glu Asn Ala Ala Gly Gly Arg Val Val Thr Ala Pro Thr Asn Gly 
        275                 280                 285             


Ala Cys Gly Ile Val Pro Ala Val Leu Ala Tyr Tyr Asp His Phe Ile 
    290                 295                 300                 


Glu Ser Val Ser Pro Asp Ile Tyr Thr Arg Tyr Phe Met Ala Ala Gly 
305                 310                 315                 320 


Ala Ile Gly Ala Leu Tyr Lys Met Asn Ala Ser Ile Ser Gly Ala Glu 
                325                 330                 335     


Val Gly Cys Gln Gly Glu Val Gly Val Ala Cys Ser Met Ala Ala Ala 
            340                 345                 350         


Gly Leu Ala Glu Leu Leu Gly Gly Ser Pro Glu Gln Val Cys Val Ala 
        355                 360                 365             


Ala Glu Ile Gly Met Glu His Asn Leu Gly Leu Thr Cys Asp Pro Val 
    370                 375                 380                 


Ala Gly Gln Val Gln Val Pro Cys Ile Glu Arg Asn Ala Ile Ala Ser 
385                 390                 395                 400 


Val Lys Ala Ile Asn Ala Ala Arg Met Ala Leu Arg Arg Thr Ser Ala 
                405                 410                 415     


Pro Arg Val Ser Leu Asp Lys Val Ile Glu Thr Met Tyr Glu Thr Gly 
            420                 425                 430         


Lys Asp Met Asn Ala Lys Tyr Arg Glu Thr Ser Arg Gly Gly Leu Ala 
        435                 440                 445             


Ile Lys Val Gln Cys Asp 
    450                 


<210>  14
<211>  455
<212>  PRT
<213>  E. coli

<400>  14

Met Ile Ser Val Phe Asp Ile Phe Lys Ile Gly Ile Gly Pro Ser Ser 
1               5                   10                  15      


Ser His Thr Val Gly Pro Met Lys Ala Gly Lys Gln Phe Thr Asp Asp 
            20                  25                  30          


Leu Ile Ala Arg Asn Leu Leu Lys Asp Val Thr Arg Val Val Val Asp 
        35                  40                  45              


Val Tyr Gly Ser Leu Ser Leu Thr Gly Lys Gly His His Thr Asp Ile 
    50                  55                  60                  


Ala Ile Ile Met Gly Leu Ala Gly Asn Leu Pro Asp Thr Val Asp Ile 
65                  70                  75                  80  


Asp Ser Ile Pro Ser Phe Ile Gln Asp Val Asn Thr His Gly Arg Leu 
                85                  90                  95      


Met Leu Ala Asn Gly Gln His Glu Val Glu Phe Pro Val Asp Gln Cys 
            100                 105                 110         


Met Asn Phe His Ala Asp Asn Leu Ser Leu His Glu Asn Gly Met Arg 
        115                 120                 125             


Ile Thr Ala Leu Ala Gly Asp Lys Val Val Tyr Ser Gln Thr Tyr Tyr 
    130                 135                 140                 


Ser Ile Gly Gly Gly Phe Ile Val Asp Glu Glu His Phe Gly Gln Gln 
145                 150                 155                 160 


Asp Ser Ala Pro Val Glu Val Pro Tyr Pro Tyr Ser Ser Ala Ala Asp 
                165                 170                 175     


Leu Gln Lys His Cys Gln Glu Thr Gly Leu Ser Leu Ser Gly Leu Met 
            180                 185                 190         


Met Lys Asn Glu Leu Ala Leu His Ser Lys Glu Glu Leu Glu Gln His 
        195                 200                 205             


Leu Ala Asn Val Trp Glu Val Met Arg Gly Gly Ile Glu Arg Gly Ile 
    210                 215                 220                 


Ser Thr Glu Gly Val Leu Pro Gly Lys Leu Arg Val Pro Arg Arg Ala 
225                 230                 235                 240 


Ala Ala Leu Arg Arg Met Leu Val Ser Gln Asp Lys Thr Thr Thr Asp 
                245                 250                 255     


Pro Met Ala Val Val Asp Trp Ile Asn Met Phe Ala Leu Ala Val Asn 
            260                 265                 270         


Glu Glu Asn Ala Ala Gly Gly Arg Val Val Thr Ala Pro Thr Asn Gly 
        275                 280                 285             


Ala Cys Gly Ile Ile Pro Ala Val Leu Ala Tyr Tyr Asp Lys Phe Ile 
    290                 295                 300                 


Arg Glu Val Asn Ala Asn Ser Leu Ala Arg Tyr Leu Leu Val Ala Ser 
305                 310                 315                 320 


Ala Ile Gly Ser Leu Tyr Lys Met Asn Ala Ser Ile Ser Gly Ala Glu 
                325                 330                 335     


Val Gly Cys Gln Gly Glu Val Gly Val Ala Cys Ser Met Ala Ala Ala 
            340                 345                 350         


Gly Leu Ala Glu Leu Leu Gly Ala Ser Pro Ala Gln Val Cys Ile Ala 
        355                 360                 365             


Ala Glu Ile Ala Met Glu His Asn Leu Gly Leu Thr Cys Asp Pro Val 
    370                 375                 380                 


Ala Gly Gln Val Gln Val Pro Cys Ile Glu Arg Asn Ala Ile Ala Ala 
385                 390                 395                 400 


Val Lys Ala Val Asn Ala Ala Arg Met Ala Leu Arg Arg Thr Ser Glu 
                405                 410                 415     


Pro Arg Val Cys Leu Asp Lys Val Ile Glu Thr Met Tyr Glu Thr Gly 
            420                 425                 430         


Lys Asp Met Asn Ala Lys Tyr Arg Glu Thr Ser Arg Gly Gly Leu Ala 
        435                 440                 445             


Met Lys Ile Val Ala Cys Asp 
    450                 455 


<210>  15
<211>  658
<212>  PRT
<213>  E. coli

<400>  15

Met Ser Asp Asp Met Ser Met Gly Leu Pro Ser Ser Ala Gly Glu His 
1               5                   10                  15      


Gly Val Leu Arg Ser Met Gln Glu Val Ala Met Ser Ser Gln Glu Ala 
            20                  25                  30          


Ser Lys Met Leu Arg Thr Tyr Asn Ile Ala Trp Trp Gly Asn Asn Tyr 
        35                  40                  45              


Tyr Asp Val Asn Glu Leu Gly His Ile Ser Val Cys Pro Asp Pro Asp 
    50                  55                  60                  


Val Pro Glu Ala Arg Val Asp Leu Ala Gln Leu Val Lys Thr Arg Glu 
65                  70                  75                  80  


Ala Gln Gly Gln Arg Leu Pro Ala Leu Phe Cys Phe Pro Gln Ile Leu 
                85                  90                  95      


Gln His Arg Leu Arg Ser Ile Asn Ala Ala Phe Lys Arg Ala Arg Glu 
            100                 105                 110         


Ser Tyr Gly Tyr Asn Gly Asp Tyr Phe Leu Val Tyr Pro Ile Lys Val 
        115                 120                 125             


Asn Gln His Arg Arg Val Ile Glu Ser Leu Ile His Ser Gly Glu Pro 
    130                 135                 140                 


Leu Gly Leu Glu Ala Gly Ser Lys Ala Glu Leu Met Ala Val Leu Ala 
145                 150                 155                 160 


His Ala Gly Met Thr Arg Ser Val Ile Val Cys Asn Gly Tyr Lys Asp 
                165                 170                 175     


Arg Glu Tyr Ile Arg Leu Ala Leu Ile Gly Glu Lys Met Gly His Lys 
            180                 185                 190         


Val Tyr Leu Val Ile Glu Lys Met Ser Glu Ile Ala Ile Val Leu Asp 
        195                 200                 205             


Glu Ala Glu Arg Leu Asn Val Val Pro Arg Leu Gly Val Arg Ala Arg 
    210                 215                 220                 


Leu Ala Ser Gln Gly Ser Gly Lys Trp Gln Ser Ser Gly Gly Glu Lys 
225                 230                 235                 240 


Ser Lys Phe Gly Leu Ala Ala Thr Gln Val Leu Gln Leu Val Glu Thr 
                245                 250                 255     


Leu Arg Glu Ala Gly Arg Leu Asp Ser Leu Gln Leu Leu His Phe His 
            260                 265                 270         


Leu Gly Ser Gln Met Ala Asn Ile Arg Asp Ile Ala Thr Gly Val Arg 
        275                 280                 285             


Glu Ser Ala Arg Phe Tyr Val Glu Leu His Lys Leu Gly Val Asn Ile 
    290                 295                 300                 


Gln Cys Phe Asp Val Gly Gly Gly Leu Gly Val Asp Tyr Glu Gly Thr 
305                 310                 315                 320 


Arg Ser Gln Ser Asp Cys Ser Val Asn Tyr Gly Leu Asn Glu Tyr Ala 
                325                 330                 335     


Asn Asn Ile Ile Trp Ala Ile Gly Asp Ala Cys Glu Glu Asn Gly Leu 
            340                 345                 350         


Pro His Pro Thr Val Ile Thr Glu Ser Gly Arg Ala Val Thr Ala His 
        355                 360                 365             


His Thr Val Leu Val Ser Asn Ile Ile Gly Val Glu Arg Asn Glu Tyr 
    370                 375                 380                 


Thr Val Pro Thr Ala Pro Ala Glu Asp Ala Pro Arg Ala Leu Gln Ser 
385                 390                 395                 400 


Met Trp Glu Thr Trp Gln Glu Met His Glu Pro Gly Thr Arg Arg Ser 
                405                 410                 415     


Leu Arg Glu Trp Leu His Asp Ser Gln Met Asp Leu His Asp Ile His 
            420                 425                 430         


Ile Gly Tyr Ser Ser Gly Ile Phe Ser Leu Gln Glu Arg Ala Trp Ala 
        435                 440                 445             


Glu Gln Leu Tyr Leu Ser Met Cys His Glu Val Gln Lys Gln Leu Asp 
    450                 455                 460                 


Pro Gln Asn Arg Ala His Arg Pro Ile Ile Asp Glu Leu Gln Glu Arg 
465                 470                 475                 480 


Met Ala Asp Lys Met Tyr Val Asn Phe Ser Leu Phe Gln Ser Met Pro 
                485                 490                 495     


Asp Ala Trp Gly Ile Asp Gln Leu Phe Pro Val Leu Pro Leu Glu Gly 
            500                 505                 510         


Leu Asp Gln Val Pro Glu Arg Arg Ala Val Leu Leu Asp Ile Thr Cys 
        515                 520                 525             


Asp Ser Asp Gly Ala Ile Asp His Tyr Ile Asp Gly Asp Gly Ile Ala 
    530                 535                 540                 


Thr Thr Met Pro Met Pro Glu Tyr Asp Pro Glu Asn Pro Pro Met Leu 
545                 550                 555                 560 


Gly Phe Phe Met Val Gly Ala Tyr Gln Glu Ile Leu Gly Asn Met His 
                565                 570                 575     


Asn Leu Phe Gly Asp Thr Glu Ala Val Asp Val Phe Val Phe Pro Asp 
            580                 585                 590         


Gly Ser Val Glu Val Glu Leu Ser Asp Glu Gly Asp Thr Val Ala Asp 
        595                 600                 605             


Met Leu Gln Tyr Val Gln Leu Asp Pro Lys Thr Leu Leu Thr Gln Phe 
    610                 615                 620                 


Arg Asp Gln Val Lys Lys Thr Asp Leu Asp Ala Glu Leu Gln Gln Gln 
625                 630                 635                 640 


Phe Leu Glu Glu Phe Glu Ala Gly Leu Tyr Gly Tyr Thr Tyr Leu Glu 
                645                 650                 655     


Asp Glu 
        


<210>  16
<211>  417
<212>  PRT
<213>  E. coli

<400>  16

Met Ser Phe Cys Trp Asn Glu Ile Asn Ser Gly Ile Lys Ser Leu Ile 
1               5                   10                  15      


Leu Ile Leu Cys Ile Phe Ser Leu Met Thr Leu Ser Leu Trp Asp Asp 
            20                  25                  30          


Val Ala Thr Lys Phe Leu His Ala Ala Gly Ile Ile Ser Ala Leu Tyr 
        35                  40                  45              


Phe Leu Ala Thr Pro Lys Lys Thr Ile Thr Asn Asn Pro Thr Leu Leu 
    50                  55                  60                  


Ile Phe Ile Ser Leu Cys Leu Leu Gly Ile Val Asn Ile Ile Trp Tyr 
65                  70                  75                  80  


Ser His Tyr Lys Val Ser Gly Ser Val Tyr Thr Asn Ala Tyr Arg Gly 
                85                  90                  95      


Pro Met Glu Thr Gly Lys Ile Ala Leu Cys Ser Ala Phe Ile Phe Leu 
            100                 105                 110         


Val Leu Phe Ala Lys Asn Glu Met Arg Thr Lys Ile Lys Phe Gly Lys 
        115                 120                 125             


Leu Ile Leu Phe Ala Ser Leu Ala Thr Gln Leu Leu Phe Phe Ala His 
    130                 135                 140                 


Ala Met Trp Gln His Phe Tyr Leu Asn Val Asp Arg Val Ala Leu Ser 
145                 150                 155                 160 


Ala Ser His Ala Thr Thr Ala Gly Tyr Ile Ile Leu Phe Pro Ser Leu 
                165                 170                 175     


Leu Ala Ser Ile Leu Ile Leu Lys Ser Asp Phe Arg His Lys Thr Thr 
            180                 185                 190         


Leu Tyr Thr Ile Asn Phe Met Leu Ser Leu Cys Ala Val Ile Val Thr 
        195                 200                 205             


Glu Thr Arg Ala Ala Ile Leu Val Phe Pro Phe Phe Ala Leu Ile Leu 
    210                 215                 220                 


Ile Val Met Asp Ser Tyr Ile Asn Lys Arg Ile Asn Tyr Lys Leu Tyr 
225                 230                 235                 240 


Cys Phe Ile Thr Ile Ala Leu Leu Ala Gly Val Phe Ser Phe Lys Asp 
                245                 250                 255     


Thr Leu Leu Met Arg Met Asn Asp Leu Asn Asn Asp Leu Val Asn Tyr 
            260                 265                 270         


Ser His Asp Asn Thr Arg Thr Ser Val Gly Ala Arg Leu Ala Met Tyr 
        275                 280                 285             


Glu Val Gly Leu Lys Thr Tyr Ser Pro Ile Gly Gln Ser Leu Glu Lys 
    290                 295                 300                 


Arg Ala Glu Lys Ile His Glu Leu Glu Glu Lys Glu Pro Arg Leu Ser 
305                 310                 315                 320 


Gly Ala Leu Pro Tyr Val Asp Ser His Leu His Asn Asp Leu Ile Asp 
                325                 330                 335     


Thr Leu Ser Thr Arg Gly Ile Pro Gly Val Val Leu Thr Ile Leu Ala 
            340                 345                 350         


Phe Ser Ala Ile Leu Ile Tyr Ala Leu Arg Thr Ala Lys Glu Pro Tyr 
        355                 360                 365             


Ile Leu Ile Leu Leu Phe Ser Leu Leu Val Val Gly Leu Ser Asp Val 
    370                 375                 380                 


Ile Leu Phe Ser Lys Pro Val Pro Thr Ala Val Phe Ile Thr Ile Ile 
385                 390                 395                 400 


Leu Leu Cys Ala Tyr Phe Lys Ala Gln Ser Asp Gln Cys Leu Leu Glu 
                405                 410                 415     


Lys 
    


<210>  17
<211>  1653
<212>  DNA
<213>  Artificial

<220>
<223>  Luciferase coding sequence adapted for expression in Clostridium 
       acetobutylicum

<400>  17
atggaggatg caaagaatat taagaaaggt ccagctcctt tctacccttt agaagacgga       60

actgctggtg agcaattaca caaggcaatg aagaggtatg cattagtacc aggaactata      120

gcttttactg atgctcatat tgaagtaaat ataacatatg cagaatactt tgagatgtct      180

gtgaggcttg cagaagcaat gaaaagatat ggattaaata ctaaccacag gatagtggtt      240

tgttctgaaa acagcttaca attcttcatg ccagttcttg gagcattatt cattggagtt      300

gctgtggctc cagcaaatga catttacaac gagagggagt tgttaaattc aatgaatatt      360

agtcaaccta ctgtagtgtt cgtttctaag aagggacttc agaaaattct aaacgtgcaa      420

aaaaagctac caattattca aaagataata attatggact caaaaactga ttaccaagga      480

ttccagagca tgtatacttt tgttacatct catctaccac caggttttaa tgagtatgat      540

ttcgtgccag aaagctttga cagagataag acaatagctt tgattatgaa cagctcagga      600

tctacaggac tacctaaggg tgtggctcta cctcatagga ctgcttgcgt taggtttagt      660

catgcaaggg accctatatt tggaaatcaa attattcctg atactgcaat actatcagtt      720

gtaccatttc atcacggatt cggtatgttc acaacattgg gatatcttat atgcggtttt      780

agagtggtac ttatgtatag gttcgaggaa gaactttttt taaggagtct acaagactac      840

aaaatacaat cagcattgtt agtgccaaca ttatttagtt ttttcgctaa aagcacactt      900

atagataaat acgacttgtc taacctacac gaaatagcaa gcggtggagc tcctttatct      960

aaagaggtag gagaagctgt tgcaaaaaga ttccacttac ctggtataag acagggttat     1020

ggattgacag aaacaacatc agcaattttg attactccag agggagatga caagccaggt     1080

gctgtaggaa aggtggtacc attctttgaa gctaaagtag tagacttgga cacaggaaaa     1140

actctaggtg tgaatcagag aggagaactt tgcgtaaggg gaccaatgat aatgtcaggt     1200

tatgtaaata atccagaggc aacaaatgca cttatagata aagatggttg gttgcacagc     1260

ggagatatag cttactggga tgaggacgaa cattttttta ttgtggacag gcttaaaagt     1320

ttgattaaat acaaaggtta ccaggtggca ccagctgagc ttgaatctat attgcttcaa     1380

cacccaaata tttttgatgc tggtgtagca ggtcttcctg atgatgatgc aggagagctt     1440

ccagctgctg tagtagtatt agagcacgga aagacaatga cagaaaagga aatagtggac     1500

tacgttgcat ctcaggttac aactgcaaag aagctaaggg gaggtgttgt ttttgtagac     1560

gaagttccta aaggattgac aggaaagttg gacgctagga agattagaga gattctaata     1620

aaagctaaga agggtggtaa aagtaagtta tag                                  1653


<210>  18
<211>  3416
<212>  DNA
<213>  Artificial

<220>
<223>  Nucleotide sequence of expression vector pJL1 + Luciferase codon 
       sequence adapted for expression in Clostridium acetobutylicum

<400>  18
agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa       60

aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc      120

cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt      180

agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc      240

tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac      300

gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca      360

gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg      420

ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag      480

gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt      540

ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat      600

ggaaaaacgc cagcaacgcg atcccgcgaa attaatacga ctcactatag ggagaccaca      660

acggtttccc tctagaaata attttgttta actttaagaa ggagatatac atatggagga      720

tgcaaagaat attaagaaag gtccagctcc tttctaccct ttagaagacg gaactgctgg      780

tgagcaatta cacaaggcaa tgaagaggta tgcattagta ccaggaacta tagcttttac      840

tgatgctcat attgaagtaa atataacata tgcagaatac tttgagatgt ctgtgaggct      900

tgcagaagca atgaaaagat atggattaaa tactaaccac aggatagtgg tttgttctga      960

aaacagctta caattcttca tgccagttct tggagcatta ttcattggag ttgctgtggc     1020

tccagcaaat gacatttaca acgagaggga gttgttaaat tcaatgaata ttagtcaacc     1080

tactgtagtg ttcgtttcta agaagggact tcagaaaatt ctaaacgtgc aaaaaaagct     1140

accaattatt caaaagataa taattatgga ctcaaaaact gattaccaag gattccagag     1200

catgtatact tttgttacat ctcatctacc accaggtttt aatgagtatg atttcgtgcc     1260

agaaagcttt gacagagata agacaatagc tttgattatg aacagctcag gatctacagg     1320

actacctaag ggtgtggctc tacctcatag gactgcttgc gttaggttta gtcatgcaag     1380

ggaccctata tttggaaatc aaattattcc tgatactgca atactatcag ttgtaccatt     1440

tcatcacgga ttcggtatgt tcacaacatt gggatatctt atatgcggtt ttagagtggt     1500

acttatgtat aggttcgagg aagaactttt tttaaggagt ctacaagact acaaaataca     1560

atcagcattg ttagtgccaa cattatttag ttttttcgct aaaagcacac ttatagataa     1620

atacgacttg tctaacctac acgaaatagc aagcggtgga gctcctttat ctaaagaggt     1680

aggagaagct gttgcaaaaa gattccactt acctggtata agacagggtt atggattgac     1740

agaaacaaca tcagcaattt tgattactcc agagggagat gacaagccag gtgctgtagg     1800

aaaggtggta ccattctttg aagctaaagt agtagacttg gacacaggaa aaactctagg     1860

tgtgaatcag agaggagaac tttgcgtaag gggaccaatg ataatgtcag gttatgtaaa     1920

taatccagag gcaacaaatg cacttataga taaagatggt tggttgcaca gcggagatat     1980

agcttactgg gatgaggacg aacatttttt tattgtggac aggcttaaaa gtttgattaa     2040

atacaaaggt taccaggtgg caccagctga gcttgaatct atattgcttc aacacccaaa     2100

tatttttgat gctggtgtag caggtcttcc tgatgatgat gcaggagagc ttccagctgc     2160

tgtagtagta ttagagcacg gaaagacaat gacagaaaag gaaatagtgg actacgttgc     2220

atctcaggtt acaactgcaa agaagctaag gggaggtgtt gtttttgtag acgaagttcc     2280

taaaggattg acaggaaagt tggacgctag gaagattaga gagattctaa taaaagctaa     2340

gaagggtggt aaaagtaagt tataggtcga ccggctgcta acaaagcccg aaaggaagct     2400

gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg     2460

gtcttgaggg gttttttgct gaaagccaat tctgattaga aaaactcatc gagcatcaaa     2520

tgaaactgca atttattcat atcaggatta tcaataccat atttttgaaa aagccgtttc     2580

tgtaatgaag gagaaaactc accgaggcag ttccatagga tggcaagatc ctggtatcgg     2640

tctgcgattc cgactcgtcc aacatcaata caacctatta atttcccctc gtcaaaaata     2700

aggttatcaa gtgagaaatc accatgagtg acgactgaat ccggtgagaa tggcaaaagc     2760

ttatgcattt ctttccagac ttgttcaaca ggccagccat tacgctcgtc atcaaaatca     2820

ctcgcatcaa ccaaaccgtt attcattcgt gattgcgcct gagcgagacg aaatacgcga     2880

tcgctgttaa aaggacaatt acaaacagga atcgaatgca accggcgcag gaacactgcc     2940

agcgcatcaa caatattttc acctgaatca ggatattctt ctaatacctg gaatgctgtt     3000

ttcccgggga tcgcagtggt gagtaaccat gcatcatcag gagtacggat aaaatgcttg     3060

atggtcggaa gaggcataaa ttccgtcagc cagtttagtc tgaccatctc atctgtaaca     3120

tcattggcaa cgctaccttt gccatgtttc agaaacaact ctggcgcatc gggcttccca     3180

tacaatcgat agattgtcgc acctgattgc ccgacattat cgcgagccca tttataccca     3240

tataaatcag catccatgtt ggaatttaat cgcggcttcg agcaagacgt ttcccgttga     3300

atatggctca taacacccct tgtattactg tttatgtaag cagacagttt tattgttcat     3360

gatgatatat ttttatcttg tgcaatgtaa catcagagat tttgagacac aacgtg         3416


<210>  19
<211>  1653
<212>  DNA
<213>  Artificial

<220>
<223>  Luciferase codon sequence adapted for expression in Clostridium 
       autoethanogenum

<400>  19
atggaagatg caaaaaatat aaagaaagga ccagcaccat tctatccact tgaagacgga       60

acagcaggag aacagctaca taaagcaatg aaaagatatg cacttgtacc gggaacaata      120

gcttttactg atgctcacat agaagtaaac ataacctatg cggaatattt tgaaatgtca      180

gtaagattgg cagaggcaat gaaaagatat ggattaaata caaatcatag aatagtagtg      240

tgtagtgaaa acagcttgca gttttttatg cctgtacttg gtgctttatt cataggtgta      300

gcagtagcac cagctaatga tatttataat gaacgtgagc ttttaaattc tatgaatata      360

agtcagccaa ctgtagtatt tgtttcaaag aaaggtttgc agaagatttt gaatgttcaa      420

aagaaattgc ctataattca aaaaataata attatggatt ctaagacaga ttatcaggga      480

ttccagtcta tgtatacatt cgtaacatct catcttcccc cgggatttaa tgaatatgac      540

ttcgtacctg aatcctttga tagagataag acaatagctt taatcatgaa tagttcagga      600

agcacaggac ttcctaaagg tgtggcactt ccacatagaa ctgcttgtgt tagattctct      660

catgctagag atccaatttt tggaaatcaa ataattccag atacagcaat actaagtgta      720

gtaccattcc atcatggatt tgggatgttt acaactcttg gatatttaat ttgtggtttt      780

agagttgtat taatgtatag atttgaggaa gaactcttcc ttcgttcact acaagactat      840

aagatacaat ctgctttact tgtaccaact ttattttcat tttttgctaa gagtactctt      900

atagataaat atgatttaag caacctgcat gaaatagcat caggcggcgc tccactatct      960

aaggaagttg gagaagctgt tgctaaaaga ttccacttac caggaatcag gcagggatat     1020

ggacttacag aaacaacttc agcaattctt attacacctg aaggagatga caagcctgga     1080

gcagtaggta aagtggtacc attctttgaa gctaaagtag tagatttaga tacaggaaaa     1140

acattgggag ttaaccagag aggagagctg tgtgtaagag gacctatgat aatgagtgga     1200

tatgtaaata atccagaagc cactaatgca ttaatagata aggatggatg gctgcattct     1260

ggtgatatag catattggga tgaagatgaa cattttttta ttgtagatag actaaaatcc     1320

ctaataaaat ataagggata ccaggtagct ccagcagaat tagaatcaat acttctgcag     1380

catccaaaca tatttgatgc aggagtagct ggattaccag atgatgatgc aggagaactt     1440

cctgctgcag tagttgtttt agagcatggc aaaactatga ctgaaaaaga gatagttgac     1500

tatgttgcaa gtcaggttac tacagcaaag aaattgagag gcggcgtagt attcgtagat     1560

gaggttccaa aaggtcttac aggaaaattg gatgcaagaa aaatacgtga aatacttata     1620

aaggcaaaga agggcggcaa atcaaaatta taa                                  1653


<210>  20
<211>  1653
<212>  DNA
<213>  Artificial

<220>
<223>  Luciferase coding sequence adapted for expressionin E. coli

<400>  20
atggaagacg ccaaaaacat aaagaaaggc ccggctccat tctatccgct agaggatgga       60

accgctggag agcaactgca taaggctatg aagagatacg ccctggttcc tggaacaatt      120

gcttttacag atgcacatat cgaggtgaac atcacgtacg cggaatactt cgaaatgtcc      180

gttcggttgg cagaagctat gaaacgatat gggctgaata caaatcacag aatcgtcgta      240

tgcagtgaaa actctcttca attctttatg ccggtgttgg gcgcgttatt tatcggagtt      300

gcagttgcgc ccgcgaacga catttataat gaacgtgaat tgctcaacag tatgaacatt      360

tcgcagccta ccgtagtgtt tgtttccaaa aaggggttgc aaaaaatttt gaacgtgcaa      420

aaaaaattac caataatcca gaaaattatt atcatggatt ctaaaacgga ttaccaggga      480

tttcagtcga tgtacacgtt cgtcacatct catctacctc ccggttttaa tgaatacgat      540

tttgtaccag agtcctttga tcgtgacaaa acaattgcac tgataatgaa ctcctctgga      600

tctactgggt tacctaaggg tgtggccctt ccgcatagaa ctgcctgcgt cagattctcg      660

catgccagag atcctatttt tggcaatcaa atcattccgg atactgcgat tttaagtgtt      720

gttccattcc atcacggttt tggaatgttt actacactcg gatatttgat atgtggattt      780

cgagtcgtct taatgtatag atttgaagaa gagctgtttt tacgatccct tcaggattac      840

aaaattcaaa gtgcgttgct agtaccaacc ctattttcat tcttcgccaa aagcactctg      900

attgacaaat acgatttatc taatttacac gaaattgctt ctgggggcgc acctctttcg      960

aaagaagtcg gggaagcggt tgcaaaacgc ttccatcttc cagggatacg acaaggatat     1020

gggctcactg agactacatc agctattctg attacacccg agggggatga taaaccgggc     1080

gcggtcggta aagttgttcc attttttgaa gcgaaggttg tggatctgga taccgggaaa     1140

acgctgggcg ttaatcagag aggcgaatta tgtgtcagag gacctatgat tatgtccggt     1200

tatgtaaaca atccggaagc gaccaacgcc ttgattgaca aggatggatg gctacattct     1260

ggagacatag cttactggga cgaagacgaa cacttcttca tagttgaccg cttgaagtct     1320

ttaattaaat acaaaggata ccaggtggcc cccgctgaat tggagtcgat attgttacaa     1380

caccccaaca tcttcgacgc gggcgtggca ggtcttcccg acgatgacgc cggtgaactt     1440

cccgccgccg ttgttgtttt ggagcacgga aagacgatga cggaaaaaga gatcgtggat     1500

tacgtcgcca gtcaagtaac aaccgcgaaa aagttgcgcg gaggagttgt gtttgtggac     1560

gaagtaccga aaggtcttac cggaaaactc gacgcaagaa aaatcagaga gatcctcata     1620

aaggccaaga agggcggaaa gtccaaattg taa                                  1653


<210>  21
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer- pJL1 linear F

<400>  21
ctgagatacc tacagcgtga gc                                                22


<210>  22
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer- pJL1 linear R

<400>  22
cgtcactcat ggtgatttct cacttg                                            26


<210>  23
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer- PS_pJL1 linear F

<400>  23
ccgaactgag atacctacag cgtgagc                                           27


<210>  24
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer- PS_pJL1 linear R

<400>  24
tcagtcgtca ctcatggtga tttctcactt g                                      31


<210>  25
<211>  488
<212>  DNA
<213>  Clostridium autoethanogenum


<220>
<221>  misc_feature
<222>  (1)..(488)
<223>  phosphotransacetylase-actetate kinase operon (pPta-Ack; 
       CAETHG_RS16490) promoter

<400>  25
ggccgcaata tgatatttat gtccattgtg aaagggatta tattcaacta ttattccagt       60

tacgttcata gaaattttcc tttctaaaat attttattcc atgtcaagaa ctctgtttat      120

ttcattaaag aactataagt acaaagtata aggcatttga aaaaataggc tagtatattg      180

attgattatt tattttaaaa tgcctaagtg aaatatatac atattataac aataaaataa      240

gtattagtgt aggattttta aatagagtat ctattttcag attaaatttt tgattatttg      300

atttacatta tataatattg agtaaagtat tgactagcaa aattttttga tactttaatt      360

tgtgaaattt cttatcaaaa gttatatttt tgaataattt ttattgaaaa atacaactaa      420

aaaggattat agtataagtg tgtgtaattt tgtgttaaat ttaaagggag gaaatgaaca      480

tgaaacat                                                               488


<210>  26
<211>  475
<212>  DNA
<213>  Clostridium autoethanogenum


<220>
<221>  misc_feature
<222>  (1)..(475)
<223>  pyruvate:formate oxidoreductase (pPFOR; CAETHG_RS14890) promoter

<400>  26
gcaaaatagt tgataataat gcagagttat aaacaaaggt gaaaagcatt acttgtattc       60

ttttttatat attattataa attaaaatga agctgtatta gaaaaaatac acacctgtaa      120

tataaaattt taaattaatt tttaattttt tcaaaatgta ttttacatgt ttagaatttt      180

gatgtatatt aaaatagtag aatacataag atacttaatt taattaaaga tagttaagta      240

cttttcaatg tgctttttta gatgtttaat acaaatcttt aattgtaaaa gaaatgctgt      300

actatttact gtactagtga cgggattaaa ctgtattaat tataaataaa aaataagtac      360

agttgtttaa aattatattt tgtattaaat ctaatagtac gatgtaagtt attttatact      420

attgctagtt taataaaaag atttaattat atacttgaaa aggagaggaa tccat           475


<210>  27
<211>  558
<212>  DNA
<213>  Clostridium autoethanogenum


<220>
<221>  misc_feature
<222>  (1)..(558)
<223>  Wood-Ljungdahl cluster (pWL; CAETHG_RS07860) promoter

<400>  27
agatagtcat aatagttcca gaatagttca atttagaaat tagactaaac ttcaaaatgt       60

ttgttaaata tataccaaac tagtatagat attttttaaa tactggactt aaacagtagt      120

aatttgccta aaaaattttt tcaatttttt ttaaaaaatc cttttcaagt tgtacattgt      180

tatggtaata tgtaattgaa gaagttatgt agtaatattg taaacgtttc ttgatttttt      240

tacatccatg tagtgcttaa aaaaccaaaa tatgtcacat gcaattgtat atttcaaata      300

acaatattta ttttctcgtt aaattcacaa ataatttatt aataatatca ataaccaaga      360

ttatacttaa atggatgttt attttttaac acttttatag taaatatatt tattttatgt      420

agtaaaaagg ttataattat aattgtattt attacaatta attaaaataa aaaatagggt      480

tttaggtaaa attaagttat tttaagaagt aattacaata aaaattgaag ttatttcttt      540

aaggagggaa ttattcat                                                    558


<210>  28
<211>  239
<212>  PRT
<213>  Clostridium autoethanogenum


<220>
<221>  MISC_FEATURE
<222>  (1)..(239)
<223>  Metabolic gene: Acetolactate decarboxylase (CAETHG_RS14410)

<400>  28

Met Asp Asp Glu Val Lys Val Pro Asn His Ile Tyr Gln Met Ser Thr 
1               5                   10                  15      


Ile Asn Ala Leu Val Ser Gly Leu Tyr Asp Gly Cys Val Ser Leu Ser 
            20                  25                  30          


Lys Leu Leu Lys Lys Gly Asn Phe Gly Ile Gly Thr Phe Lys Gly Leu 
        35                  40                  45              


Asp Gly Glu Leu Thr Leu Leu Asn Gly Thr Phe Tyr Arg Thr Lys Pro 
    50                  55                  60                  


Asp Gly Ser Val Tyr Val Cys Ser Lys Asn Val Ser Val Pro Phe Ala 
65                  70                  75                  80  


Val Val Thr Glu Leu Glu Asn Tyr Asn Thr Tyr Asn Ile Gln Asn Arg 
                85                  90                  95      


Thr Ser Tyr Glu Asp Ile Arg Lys Glu Leu Asp Ser Phe Ile Glu Ser 
            100                 105                 110         


Lys Asn Ile Phe Tyr Ala Phe Tyr Met Glu Gly Lys Phe Asn Tyr Val 
        115                 120                 125             


Lys Thr Arg Thr Val Val Lys Gln Asn Met Pro Tyr Lys Pro Met Ala 
    130                 135                 140                 


Glu Val Val Lys Asp Gln Pro Met Phe Glu Tyr Asn Gly Val Asp Gly 
145                 150                 155                 160 


Tyr Val Val Gly Phe Arg Cys Pro Asp Tyr Val Glu Gly Leu Asn Val 
                165                 170                 175     


Pro Gly Tyr His Phe His Phe Ile Asn Lys Asp Lys Lys Phe Gly Gly 
            180                 185                 190         


His Ile Ser Glu Phe Ser Ile Glu Asn Ala Lys Val Tyr Val Gln Asn 
        195                 200                 205             


Cys Ser Cys Phe Arg Met Glu Leu Pro Lys Asn Glu Ser Phe Tyr Asn 
    210                 215                 220                 


Met Glu Val Gln Asp Arg Asn Asp Glu Ile Thr Ser Val Glu Lys 
225                 230                 235                 


<210>  29
<211>  556
<212>  PRT
<213>  Clostridium autoethanogenum


<220>
<221>  MISC_FEATURE
<222>  (1)..(556)
<223>  Metabolic gene: Acetolactate synthase (CAETHG_RS08420)

<400>  29

Met Asn Arg Asp Ile Lys Lys Glu Val Gln Leu Asn Thr Ala Gln Met 
1               5                   10                  15      


Leu Val Lys Cys Leu Glu Ala Glu Gly Val Lys Tyr Ile Phe Gly Ile 
            20                  25                  30          


Pro Gly Glu Glu Asn Leu Glu Ile Met Asn Ala Ile Ser Asp Ser Thr 
        35                  40                  45              


Ile Glu Phe Ile Thr Thr Arg His Glu Gln Gly Ala Ala Phe Met Ala 
    50                  55                  60                  


Asp Val Tyr Gly Arg Leu Thr Gly Lys Ala Gly Val Cys Leu Ser Thr 
65                  70                  75                  80  


Leu Gly Pro Gly Ala Thr Asn Leu Val Thr Gly Val Ala Asp Ala Asp 
                85                  90                  95      


Ser Asp Gly Ala Pro Val Val Ala Ile Thr Gly Gln Val Gly Thr Glu 
            100                 105                 110         


Arg Met His Ile Thr Ser His Gln Phe Leu Asp Leu Cys Lys Met Phe 
        115                 120                 125             


Glu Pro Ile Thr Lys Arg Ser Lys Gln Ile Val Arg Pro Asp Thr Val 
    130                 135                 140                 


Ser Glu Ile Ile Arg Leu Val Phe Lys Tyr Ala Glu Ser Glu Lys Pro 
145                 150                 155                 160 


Gly Ala Cys His Ile Asp Leu Pro Val Asn Ile Ala Lys Met Pro Val 
                165                 170                 175     


Gly Ala Leu Glu Lys Pro Leu Glu Lys Lys Ile Pro Pro Lys Glu His 
            180                 185                 190         


Ala Asp Leu Ser Thr Ile Glu Glu Ala Ala Ser Glu Ile Phe Lys Ala 
        195                 200                 205             


Lys Asn Pro Ile Ile Leu Ala Gly Ser Gly Ala Ile Arg Gly Asn Ser 
    210                 215                 220                 


Ser Lys Ala Val Thr Glu Phe Ala Thr Lys Leu Lys Ile Pro Val Ile 
225                 230                 235                 240 


Asn Thr Met Met Ala Lys Gly Ile Ile Pro Met Asp Asn Lys Tyr Ser 
                245                 250                 255     


Met Trp Thr Ile Gly Ile Pro Gln Lys Asp Tyr Val Asn Lys Ile Ile 
            260                 265                 270         


Glu Glu Ala Asp Leu Val Ile Thr Ile Gly Tyr Asp Ile Val Glu Tyr 
        275                 280                 285             


Ala Pro Ser Lys Trp Asn Ile Asn Gly Asp Ile Lys Ile Val His Ile 
    290                 295                 300                 


Asp Ala Arg Pro Ser His Ile Asn Lys Leu Tyr Gln Pro Ile Val Glu 
305                 310                 315                 320 


Val Val Gly Asp Ile Ser Asp Ala Leu Tyr Asn Ile Leu Arg Arg Thr 
                325                 330                 335     


Ser Ser Lys Asp Glu Pro Val Lys Ala Leu Glu Ile Lys Ser Glu Met 
            340                 345                 350         


Leu Ala Glu His Glu Ser Tyr Ala Asn Asp Asn Ala Phe Pro Met Lys 
        355                 360                 365             


Pro Gln Arg Ile Leu Asn Asp Val Arg Lys Val Met Gly Pro His Asp 
    370                 375                 380                 


Ile Val Ile Ser Asp Val Gly Ala His Lys Met Trp Ile Ala Arg His 
385                 390                 395                 400 


Tyr Asn Cys Tyr Glu Pro Asn Thr Cys Ile Ile Ser Asn Gly Phe Ala 
                405                 410                 415     


Thr Met Gly Ile Gly Val Pro Gly Ala Ile Ala Ala Lys Leu Ile Asn 
            420                 425                 430         


Pro Asp Lys Lys Val Leu Ala Ile Val Gly Asp Gly Gly Phe Met Met 
        435                 440                 445             


Asn Asn Gln Glu Leu Glu Thr Ala Leu Arg Ile Lys Thr Pro Ile Val 
    450                 455                 460                 


Val Leu Ile Phe Asn Asp Ser Asn Tyr Gly Leu Ile Lys Trp Lys Gln 
465                 470                 475                 480 


Glu Glu His Tyr Gly Lys Ser Cys Tyr Val Asp Phe Thr Asn Pro Asp 
                485                 490                 495     


Phe Val Lys Leu Ala Glu Ser Met Tyr Ala Lys Gly Tyr Arg Val Glu 
            500                 505                 510         


Lys Ala Glu Asp Leu Ile Pro Thr Leu Glu Glu Ala Phe Lys Gln Asn 
        515                 520                 525             


Val Pro Ala Val Ile Asp Cys Gln Val Asp Tyr Gly Glu Asn Ile Lys 
    530                 535                 540                 


Leu Thr Lys His Leu Lys Glu Val Tyr Glu Asn Met 
545                 550                 555     


<210>  30
<211>  351
<212>  PRT
<213>  Clostridium autoethanogenum


<220>
<221>  MISC_FEATURE
<222>  (1)..(351)
<223>  Metabolic gene: Primary:secondary alcohol dehydrogenase 
       (CAETHG_RS02620)

<400>  30

Met Lys Gly Phe Ala Met Leu Gly Ile Asn Lys Leu Gly Trp Ile Glu 
1               5                   10                  15      


Lys Lys Asn Pro Val Pro Gly Pro Tyr Asp Ala Ile Val His Pro Leu 
            20                  25                  30          


Ala Val Ser Pro Cys Thr Ser Asp Ile His Thr Val Phe Glu Gly Ala 
        35                  40                  45              


Leu Gly Asn Arg Glu Asn Met Ile Leu Gly His Glu Ala Val Gly Glu 
    50                  55                  60                  


Ile Ala Glu Val Gly Ser Glu Val Lys Asp Phe Lys Val Gly Asp Arg 
65                  70                  75                  80  


Val Ile Val Pro Cys Thr Thr Pro Asp Trp Arg Ser Leu Glu Val Gln 
                85                  90                  95      


Ala Gly Phe Gln Gln His Ser Asn Gly Met Leu Ala Gly Trp Lys Phe 
            100                 105                 110         


Ser Asn Phe Lys Asp Gly Val Phe Ala Asp Tyr Phe His Val Asn Asp 
        115                 120                 125             


Ala Asp Met Asn Leu Ala Ile Leu Pro Asp Glu Ile Pro Leu Glu Ser 
    130                 135                 140                 


Ala Val Met Met Thr Asp Met Met Thr Thr Gly Phe His Gly Ala Glu 
145                 150                 155                 160 


Leu Ala Asp Ile Lys Met Gly Ser Ser Val Val Val Ile Gly Ile Gly 
                165                 170                 175     


Ala Val Gly Leu Met Gly Ile Ala Gly Ser Lys Leu Arg Gly Ala Gly 
            180                 185                 190         


Arg Ile Ile Gly Val Gly Ser Arg Pro Val Cys Val Glu Thr Ala Lys 
        195                 200                 205             


Phe Tyr Gly Ala Thr Asp Ile Val Asn Tyr Lys Asn Gly Asp Ile Val 
    210                 215                 220                 


Glu Gln Ile Met Asp Leu Thr His Gly Lys Gly Val Asp Arg Val Ile 
225                 230                 235                 240 


Met Ala Gly Gly Gly Ala Glu Thr Leu Ala Gln Ala Val Thr Met Val 
                245                 250                 255     


Lys Pro Gly Gly Val Ile Ser Asn Ile Asn Tyr His Gly Ser Gly Asp 
            260                 265                 270         


Thr Leu Pro Ile Pro Arg Val Gln Trp Gly Cys Gly Met Ala His Lys 
        275                 280                 285             


Thr Ile Arg Gly Gly Leu Cys Pro Gly Gly Arg Leu Arg Met Glu Met 
    290                 295                 300                 


Leu Arg Asp Leu Val Leu Tyr Lys Arg Val Asp Leu Ser Lys Leu Val 
305                 310                 315                 320 


Thr His Val Phe Asp Gly Ala Glu Asn Ile Glu Lys Ala Leu Leu Leu 
                325                 330                 335     


Met Lys Asn Lys Pro Lys Asp Leu Ile Lys Ser Val Val Thr Phe 
            340                 345                 350     


