                         SEQUENCE LISTING

<110>  DUKE UNIVERSITY
       SUN, Baodong
       KISHNANI, Priya
       LIM, Jeong-A
 
<120>  COMPOSITIONS AND METHODS FOR THE TREATMENT OF HUMAN GENETIC 
       DISEASES

<130>  19-331-WO

<150>  US 62/823,894
<151>  2019-03-26

<150>  US 62/807,790
<151>  2019-02-20

<160>  38    

<170>  PatentIn version 3.5

<210>  1
<211>  718
<212>  PRT
<213>  Bacillus subtilis

<400>  1

Met Val Ser Ile Arg Arg Ser Phe Glu Ala Tyr Val Asp Asp Met Asn 
1               5                   10                  15      


Ile Ile Thr Val Leu Ile Pro Ala Glu Gln Lys Glu Ile Met Thr Pro 
            20                  25                  30          


Pro Phe Arg Leu Glu Thr Glu Ile Thr Asp Phe Pro Leu Ala Val Arg 
        35                  40                  45              


Glu Glu Tyr Ser Leu Glu Ala Lys Tyr Lys Tyr Val Cys Val Ser Asp 
    50                  55                  60                  


His Pro Val Thr Phe Gly Lys Ile His Cys Val Arg Ala Ser Ser Gly 
65                  70                  75                  80  


His Lys Thr Asp Leu Gln Ile Gly Ala Val Ile Arg Thr Ala Ala Phe 
                85                  90                  95      


Asp Asp Glu Phe Tyr Tyr Asp Gly Glu Leu Gly Ala Val Tyr Thr Ala 
            100                 105                 110         


Asp His Thr Val Phe Lys Val Trp Ala Pro Ala Ala Thr Ser Ala Ala 
        115                 120                 125             


Val Lys Leu Ser His Pro Asn Lys Ser Gly Arg Thr Phe Gln Met Thr 
    130                 135                 140                 


Arg Leu Glu Lys Gly Val Tyr Ala Val Thr Val Thr Gly Asp Leu His 
145                 150                 155                 160 


Gly Tyr Glu Tyr Leu Phe Cys Ile Cys Asn Asn Ser Glu Trp Met Glu 
                165                 170                 175     


Thr Val Asp Gln Tyr Ala Lys Ala Val Thr Val Asn Gly Glu Lys Gly 
            180                 185                 190         


Val Val Leu Arg Pro Asp Gln Met Lys Trp Thr Ala Pro Leu Lys Pro 
        195                 200                 205             


Phe Ser His Pro Val Asp Ala Val Ile Tyr Glu Thr His Leu Arg Asp 
    210                 215                 220                 


Phe Ser Ile His Glu Asn Ser Gly Met Ile Asn Lys Gly Lys Tyr Leu 
225                 230                 235                 240 


Ala Leu Thr Glu Thr Asp Thr Gln Thr Ala Asn Gly Ser Ser Ser Gly 
                245                 250                 255     


Leu Ala Tyr Val Lys Glu Leu Gly Val Thr His Val Glu Leu Leu Pro 
            260                 265                 270         


Val Asn Asp Phe Ala Gly Val Asp Glu Glu Lys Pro Leu Asp Ala Tyr 
        275                 280                 285             


Asn Trp Gly Tyr Asn Pro Leu His Phe Phe Ala Pro Glu Gly Ser Tyr 
    290                 295                 300                 


Ala Ser Asn Pro His Asp Pro Gln Thr Arg Lys Thr Glu Leu Lys Gln 
305                 310                 315                 320 


Met Ile Asn Thr Leu His Gln His Gly Leu Arg Val Ile Leu Asp Val 
                325                 330                 335     


Val Phe Asn His Val Tyr Lys Arg Glu Asn Ser Pro Phe Glu Lys Thr 
            340                 345                 350         


Val Pro Gly Tyr Phe Phe Arg His Asp Glu Cys Gly Met Pro Ser Asn 
        355                 360                 365             


Gly Thr Gly Val Gly Asn Asp Ile Ala Ser Glu Arg Arg Met Ala Arg 
    370                 375                 380                 


Lys Phe Ile Ala Asp Cys Val Val Tyr Trp Leu Glu Glu Tyr Asn Val 
385                 390                 395                 400 


Asp Gly Phe Arg Phe Asp Leu Leu Gly Ile Leu Asp Ile Asp Thr Val 
                405                 410                 415     


Leu Tyr Met Lys Glu Lys Ala Thr Lys Ala Lys Pro Gly Ile Leu Leu 
            420                 425                 430         


Phe Gly Glu Gly Trp Asp Leu Ala Thr Pro Leu Pro His Glu Gln Lys 
        435                 440                 445             


Ala Ala Leu Ala Asn Ala Pro Arg Met Pro Gly Ile Gly Phe Phe Asn 
    450                 455                 460                 


Asp Met Phe Arg Asp Ala Val Lys Gly Asn Thr Phe His Leu Lys Ala 
465                 470                 475                 480 


Thr Gly Phe Ala Leu Gly Asn Gly Glu Ser Ala Gln Ala Val Met His 
                485                 490                 495     


Gly Ile Ala Gly Ser Ser Gly Trp Lys Ala Leu Ala Pro Ile Val Pro 
            500                 505                 510         


Glu Pro Ser Gln Ser Ile Asn Tyr Val Glu Ser His Asp Asn His Thr 
        515                 520                 525             


Phe Trp Asp Lys Met Ser Phe Ala Leu Pro Gln Glu Asn Asp Ser Arg 
    530                 535                 540                 


Lys Arg Ser Arg Gln Arg Leu Ala Ala Ala Ile Ile Leu Leu Ala Gln 
545                 550                 555                 560 


Gly Val Pro Phe Ile His Ser Gly Gln Glu Phe Phe Arg Thr Lys Gln 
                565                 570                 575     


Gly Val Glu Asn Ser Tyr Gln Ser Ser Asp Ser Ile Asn Gln Leu Asp 
            580                 585                 590         


Trp Asp Arg Arg Glu Thr Phe Lys Glu Asp Val His Tyr Ile Arg Arg 
        595                 600                 605             


Leu Ile Ser Leu Arg Lys Ala His Pro Ala Phe Arg Leu Arg Ser Ala 
    610                 615                 620                 


Ala Asp Ile Gln Arg His Leu Glu Cys Leu Thr Leu Lys Glu His Leu 
625                 630                 635                 640 


Ile Ala Tyr Arg Leu Tyr Asp Leu Asp Glu Val Asp Glu Trp Lys Asp 
                645                 650                 655     


Ile Ile Val Ile His His Ala Ser Pro Asp Ser Val Glu Trp Arg Leu 
            660                 665                 670         


Pro Asn Asp Ile Pro Tyr Arg Leu Leu Cys Asp Pro Ser Gly Phe Gln 
        675                 680                 685             


Glu Asp Pro Thr Glu Ile Lys Lys Thr Val Ala Val Asn Gly Ile Gly 
    690                 695                 700                 


Thr Val Ile Leu Tyr Leu Ala Ser Asp Leu Lys Ser Phe Ala 
705                 710                 715             


<210>  2
<211>  2157
<212>  DNA
<213>  Bacillus subtilis

<400>  2
atggtcagca tccgccgcag cttcgaagcg tatgtcgatg acatgaatat cattactgtt       60

ctgattcctg ctgaacaaaa ggaaatcatg acaccgccgt ttcggcttga gacagaaata      120

acagattttc ctctggctgt cagggaggaa tactcccttg aagcaaaata caagtacgtc      180

tgcgtatccg accatcctgt gacatttgga aaaatccatt gcgtcagagc atccagcggc      240

cacaaaacgg atctccaaat tggcgcggtc atccggacgg cagcgtttga tgacgaattt      300

tattatgacg gagagctggg cgccgtttat accgcggatc ataccgtatt taaagtatgg      360

gcgcctgctg caacctcagc tgctgtcaag ctttcacacc ccaataaaag cgggcgcaca      420

ttccaaatga ctcgcttgga aaaaggcgtc tatgccgtta cggtcacagg tgaccttcac      480

ggatatgagt atttgttttg catctgcaac aattcagaat ggatggaaac agttgaccag      540

tatgccaagg ctgtgactgt aaatggagag aagggcgtcg tcttgcgccc ggatcaaatg      600

aaatggactg ctcctcttaa accattctca caccctgtgg atgccgtcat ctatgagacg      660

catcttcgcg acttctccat ccatgaaaac agcggcatga taaacaaggg aaaatactta      720

gcgctgacgg aaactgatac acaaaccgca aatggcagtt cttcgggatt agcgtatgta      780

aaagagcttg gtgtgacaca tgtggagctt ctgccggtga atgattttgc cggagttgat      840

gaagagaagc cgcttgatgc atacaattgg ggatataacc cgcttcattt ctttgccccg      900

gagggaagct atgcctcaaa tcctcatgat cctcaaacga gaaaaacaga gctgaaacaa      960

atgatcaata ccctgcatca gcacggtctg cgagtcattc tggatgttgt ttttaaccat     1020

gtgtataaga gggagaattc cccctttgaa aagacagtgc ccggttattt tttccggcac     1080

gacgaatgtg ggatgccatc aaacggcacc ggcgttggca atgatattgc atcagaaaga     1140

aggatggcaa gaaaattcat tgcggattgc gtggtctatt ggcttgaaga atacaatgtt     1200

gacggcttcc gctttgatct cctcgggatt ttagatattg acaccgtgct ttatatgaaa     1260

gagaaagcaa ctaaggcaaa gcccggaatc ctgctttttg gagaagggtg ggacctggct     1320

acaccgctgc cgcatgaaca gaaagctgct ttggcgaacg cgccaagaat gccgggcatc     1380

ggctttttta atgatatgtt tcgtgacgct gtaaaaggga acacctttca ccttaaggca     1440

acagggtttg cgctcggcaa cggtgaatca gcacaagctg tgatgcatgg aattgccggg     1500

tcttccggat ggaaggcatt agcaccgatt gttccggaac caagccagtc catcaattat     1560

gtcgaatcac acgacaatca caccttttgg gataaaatga gctttgcgct tcctcaagaa     1620

aatgacagcc gaaagcgaag caggcaaagg cttgcagccg cgattatttt gcttgcccaa     1680

ggggtgccgt ttattcacag cggccaggaa tttttccgga cgaagcaggg agtggaaaac     1740

agctatcaat ccagtgacag catcaaccag ctcgactggg atcgccgtga aacattcaaa     1800

gaagatgttc actatatccg caggctgatc tcgctgagaa aagcgcatcc tgcattccgt     1860

cttaggtccg ctgcagacat ccagcgccat cttgaatgct tgacgctaaa agaacacctt     1920

atcgcataca ggctttatga tcttgacgag gttgacgaat ggaaagatat cattgttatc     1980

catcacgcga gtccagactc cgtcgagtgg aggctgccaa acgacatacc ttatcggctt     2040

ttatgtgatc catcaggatt tcaggaagac ccaacagaaa tcaagaaaac ggttgcagta     2100

aacggcatcg gaacggttat cttatattta gcatcagatc ttaagagttt tgcttga        2157


<210>  3
<211>  2157
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  codon optimized pullulanase sequence derived from B. subtilis 
       strain 168

<400>  3
atggtgagca tccggagatc cttcgaggcc tacgtggacg atatgaacat catcaccgtg       60

ctgatcccag ccgagcagaa ggagatcatg acaccccctt tccggctgga gaccgagatc      120

acagactttc ccctggccgt gagagaggag tatagcctgg aggccaagta caagtacgtg      180

tgcgtgagcg atcaccctgt gacctttggc aagatccact gcgtgcgggc aagctccgga      240

cacaagaccg acctgcagat cggagccgtg atcaggacag cagccttcga cgatgagttt      300

tactatgacg gagagctggg agccgtgtac accgcagatc acacagtgtt caaggtctgg      360

gcaccagcag ccacatccgc cgcagtgaag ctgagccacc ccaacaagtc cggcaggacc      420

tttcagatga cacgcctgga gaagggcgtg tacgccgtga ccgtgacagg cgatctgcac      480

ggctacgagt atctgttctg catctgtaac aattctgagt ggatggagac cgtggatcag      540

tatgccaagg ccgtgacagt gaatggagag aagggagtgg tgctgaggcc agaccagatg      600

aagtggaccg cacccctgaa gcctttcagc caccctgtgg acgccgtgat ctacgagaca      660

cacctgcgcg atttttctat ccacgagaac agcggcatga tcaataaggg caagtacctg      720

gccctgaccg agacagacac ccagacagcc aacggctcta gctccggcct ggcctatgtg      780

aaggagctgg gagtgaccca cgtggagctg ctgcctgtga atgactttgc cggcgtggat      840

gaggagaagc cactggatgc ctacaactgg ggctataatc cactgcactt ctttgccccc      900

gagggctctt atgccagcaa cccacacgac ccccagacca ggaagacaga gctgaagcag      960

atgatcaata cactgcacca gcacggcctg agagtgatcc tggatgtggt gttcaaccac     1020

gtgtacaagc gcgagaatag cccttttgag aagaccgtgc caggctattt ctttcggcac     1080

gacgagtgcg gcatgccatc taacggcaca ggcgtgggca atgatatcgc cagcgagagg     1140

cgcatggccc ggaagttcat cgccgactgc gtggtgtact ggctggagga gtataacgtg     1200

gacggcttca gatttgatct gctgggcatc ctggacatcg ataccgtgct gtacatgaag     1260

gagaaggcca caaaggccaa gccaggcatc ctgctgttcg gagagggatg ggacctggca     1320

accccactgc cacacgagca gaaggccgcc ctggcaaacg cacctaggat gccaggcatc     1380

ggcttcttta acgacatgtt tcgcgatgcc gtgaagggca ataccttcca cctgaaggcc     1440

acaggctttg cactgggaaa tggagagtcc gcccaggccg tgatgcacgg aatcgcagga     1500

tctagcggat ggaaggccct ggcaccaatc gtgcctgagc caagccagtc catcaactac     1560

gtggagtccc acgacaatca caccttctgg gataagatgt cttttgccct gcctcaggag     1620

aatgattcta ggaagagaag caggcagcgc ctggcagcag caatcatcct gctggcccag     1680

ggcgtgccat tcatccacag cggccaggag ttctttcgga ccaagcaggg cgtggagaac     1740

tcctaccagt cctctgattc tatcaatcag ctggactggg atcggagaga gacattcaag     1800

gaggacgtgc actatatcag gcgcctgatc agcctgagaa aggcacaccc agcctttcgg     1860

ctgagatccg ccgcagacat ccagaggcac ctggagtgcc tgaccctgaa ggagcacctg     1920

atcgcctaca gactgtatga cctggatgag gtggacgagt ggaaggatat catcgtgatc     1980

caccacgcct cccctgactc tgtggagtgg cggctgccca acgatatccc ttacagactg     2040

ctgtgcgacc cctccggctt ccaggaggat cctaccgaga tcaagaagac agtggccgtg     2100

aatggcatcg gcaccgtgat cctgtatctg gcctccgacc tgaagtcttt tgcctga        2157


<210>  4
<211>  657
<212>  PRT
<213>  Escherichia coli

<400>  4

Met Thr Gln Leu Ala Ile Gly Lys Pro Ala Pro Leu Gly Ala His Tyr 
1               5                   10                  15      


Asp Gly Gln Gly Val Asn Phe Thr Leu Phe Ser Ala His Ala Glu Arg 
            20                  25                  30          


Val Glu Leu Cys Val Phe Asp Ala Asn Gly Gln Glu His Arg Tyr Asp 
        35                  40                  45              


Leu Pro Gly His Ser Gly Asp Ile Trp His Gly Tyr Leu Pro Asp Ala 
    50                  55                  60                  


Arg Pro Gly Leu Arg Tyr Gly Tyr Arg Val His Gly Pro Trp Gln Pro 
65                  70                  75                  80  


Ala Glu Gly His Arg Phe Asn Pro Ala Lys Leu Leu Ile Asp Pro Cys 
                85                  90                  95      


Ala Arg Gln Ile Asp Gly Glu Phe Lys Asp Asn Pro Leu Leu His Ala 
            100                 105                 110         


Gly His Asn Glu Pro Asp Tyr Arg Asp Asn Ala Ala Ile Ala Pro Lys 
        115                 120                 125             


Cys Val Val Val Val Asp His Tyr Asp Trp Glu Asp Asp Ala Pro Pro 
    130                 135                 140                 


Arg Thr Pro Trp Gly Ser Thr Ile Ile Tyr Glu Ala His Val Lys Gly 
145                 150                 155                 160 


Leu Thr Tyr Leu His Pro Glu Ile Pro Val Glu Ile Arg Gly Thr Tyr 
                165                 170                 175     


Lys Ala Leu Gly His Pro Val Met Ile Asn Tyr Leu Lys Gln Leu Gly 
            180                 185                 190         


Ile Thr Ala Leu Glu Leu Leu Pro Val Ala Gln Phe Ala Ser Glu Pro 
        195                 200                 205             


Arg Leu Gln Arg Met Gly Leu Ser Asn Tyr Trp Gly Tyr Asn Pro Val 
    210                 215                 220                 


Ala Met Phe Ala Leu His Pro Ala Tyr Ala Cys Ser Pro Glu Thr Ala 
225                 230                 235                 240 


Leu Asp Glu Phe Arg Asp Ala Ile Lys Ala Leu His Lys Ala Gly Ile 
                245                 250                 255     


Glu Val Ile Leu Asp Ile Val Leu Asn His Ser Ala Glu Leu Asp Leu 
            260                 265                 270         


Asp Gly Pro Leu Phe Ser Leu Arg Gly Ile Asp Asn Arg Ser Tyr Tyr 
        275                 280                 285             


Trp Ile Arg Glu Asp Gly Asp Tyr His Asn Trp Thr Gly Cys Gly Asn 
    290                 295                 300                 


Thr Leu Asn Leu Ser His Pro Ala Val Val Asp Tyr Ala Ser Ala Cys 
305                 310                 315                 320 


Leu Arg Tyr Trp Val Glu Thr Cys His Val Asp Gly Phe Arg Phe Asp 
                325                 330                 335     


Leu Ala Ala Val Met Gly Arg Thr Pro Glu Phe Arg Gln Asp Ala Pro 
            340                 345                 350         


Leu Phe Thr Ala Ile Gln Asn Cys Pro Val Leu Ser Gln Val Lys Leu 
        355                 360                 365             


Ile Ala Glu Pro Trp Asp Ile Ala Pro Gly Gly Tyr Gln Val Gly Asn 
    370                 375                 380                 


Phe Pro Pro Leu Phe Ala Glu Trp Asn Asp His Phe Arg Asp Ala Ala 
385                 390                 395                 400 


Arg Arg Phe Trp Leu His Tyr Asp Leu Pro Leu Gly Ala Phe Ala Gly 
                405                 410                 415     


Arg Phe Ala Ala Ser Ser Asp Val Phe Lys Arg Asn Gly Arg Leu Pro 
            420                 425                 430         


Ser Ala Ala Ile Asn Leu Val Thr Ala His Asp Gly Phe Thr Leu Arg 
        435                 440                 445             


Asp Cys Val Cys Phe Asn His Lys His Asn Glu Ala Asn Gly Glu Glu 
    450                 455                 460                 


Asn Arg Asp Gly Thr Asn Asn Asn Tyr Ser Asn Asn His Gly Lys Glu 
465                 470                 475                 480 


Gly Leu Gly Gly Ser Leu Asp Leu Val Glu Arg Arg Arg Asp Ser Ile 
                485                 490                 495     


His Ala Leu Leu Thr Thr Leu Leu Leu Ser Gln Gly Thr Pro Met Leu 
            500                 505                 510         


Leu Ala Gly Asp Glu His Gly His Ser Gln His Gly Asn Asn Asn Ala 
        515                 520                 525             


Tyr Cys Gln Asp Asn Gln Leu Thr Trp Leu Asp Trp Ser Gln Ala Ser 
    530                 535                 540                 


Ser Gly Leu Thr Ala Phe Thr Ala Ala Leu Ile His Leu Arg Lys Arg 
545                 550                 555                 560 


Ile Pro Ala Leu Val Glu Asn Arg Trp Trp Glu Glu Gly Asp Gly Asn 
                565                 570                 575     


Val Arg Trp Leu Asn Arg Tyr Ala Gln Pro Leu Ser Thr Asp Glu Trp 
            580                 585                 590         


Gln Asn Gly Pro Lys Gln Leu Gln Ile Leu Leu Ser Asp Arg Phe Leu 
        595                 600                 605             


Ile Ala Ile Asn Ala Thr Leu Glu Val Thr Glu Ile Val Leu Pro Ala 
    610                 615                 620                 


Gly Glu Trp His Ala Ile Pro Pro Phe Ala Gly Glu Asp Asn Pro Val 
625                 630                 635                 640 


Ile Thr Ala Val Trp Gln Gly Pro Ala His Gly Leu Cys Val Phe Gln 
                645                 650                 655     


Arg 
    


<210>  5
<211>  1974
<212>  DNA
<213>  Escherichia coli

<400>  5
atgacacaac tcgccattgg caaacccgct cccctcggcg cgcattacga cggtcagggc       60

gtcaacttca cacttttctc cgctcatgcc gagcgggtag aactgtgtgt ctttgacgcc      120

aatggccagg aacatcgcta tgacttgcca gggcacagtg gcgacatttg gcacggttat      180

ctgccggatg cgcgcccggg tttgcgttat ggttatcgcg ttcatggccc ctggcaaccc      240

gccgaggggc atcgctttaa cccggcgaag ttgttgattg atccttgcgc gcggcaaatt      300

gacggggagt ttaaagataa cccgctgctg cacgccggtc ataatgaacc tgactatcgc      360

gacaacgccg ccattgcgcc gaaatgcgta gtggtggttg atcactatga ctgggaagat      420

gatgccccgc cgcgcacgcc gtggggcagc accatcattt atgaagccca tgtcaaagga      480

ttaacgtact tgcacccgga gatcccggtc gagatccgtg gcacttataa agccctcggg      540

catccggtga tgatcaacta tttgaaacaa ttgggcatta ccgcgctgga actgctgcca      600

gtggcgcagt ttgccagtga accacgtctg caacgcatgg ggctaagtaa ctactggggt      660

tacaacccgg tggcgatgtt tgcgctgcat ccggcgtatg cctgctcgcc agaaacggcg      720

ctggatgagt ttcgcgatgc aatcaaagca ctgcataaag cgggtatcga agtcattctt      780

gatatcgtgc tcaaccatag tgcggaactg gacctcgacg gcccgttatt ctcgctgcgt      840

gggatcgata accgtagcta ttattggata agagaagacg gcgattatca caactggacc      900

ggttgcggca acacgctcaa tttgagtcat ccggcggtgg tggattatgc cagcgcctgc      960

ctgcgttatt gggtagaaac ctgccacgtc gatggtttcc gctttgatct ggcggcagtc     1020

atgggccgta cgccagagtt ccgtcaggat gcgccgttgt ttaccgctat ccagaactgc     1080

ccggtgctct cgcaggtgaa gttaattgct gaaccgtggg atatcgctcc tggtggttat     1140

caggtgggaa atttcccgcc gctgtttgcc gagtggaacg atcatttccg cgatgctgcc     1200

cgtcgtttct ggctacatta tgatttgcct ctgggggcgt ttgccgggcg ttttgctgcc     1260

tccagcgatg tttttaaacg taatggtcgt ctgccgagtg ccgcgattaa tctcgtcacc     1320

gcgcatgacg gttttacgct tcgcgactgc gtttgcttca accataaaca caatgaagca     1380

aacggagaag aaaatcgcga cgggaccaac aacaattaca gtaacaatca tggtaaagaa     1440

gggttaggcg gttctcttga cctggttgaa cggcggcgcg acagcattca cgccctgtta     1500

acaacgttgt tgctctccca gggtacgccg atgttactgg ccggtgacga acatggtcac     1560

agccagcatg gcaataacaa tgcctactgt caggataacc aattaacctg gttggactgg     1620

tcgcaggcaa gcagtggttt aaccgcattt accgccgcgt taatccatct gcgcaagcgc     1680

attcccgctt tggtggagaa tcgctggtgg gaagaaggcg acggcaatgt ccgttggcta     1740

aatcgatatg ctcaaccttt aagcacggat gagtggcaaa acgggccgaa acagctgcaa     1800

attctgctct cggatcgctt tttgatcgca attaacgcca cgcttgaggt aacagagatt     1860

gttttacctg ctggggagtg gcacgccatt cccccattcg ctggagagga taacccagtg     1920

attacggctg tctggcaggg acctgcacac ggattgtgtg tgttccagag atga           1974


<210>  6
<211>  1974
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  codon optimized glgX sequence derived from E. coli strain K-12

<400>  6
atgacccagc tggcaatcgg caagccagca cctctgggag cccactacga cggccagggc       60

gtgaacttca cactgttttc cgcccacgca gagagggtgg agctgtgcgt gttcgatgcc      120

aatggccagg agcacagata cgacctgccc ggccactctg gcgatatctg gcacggctat      180

ctgccagacg caaggcctgg actgagatac ggctatagag tgcacggacc atggcagcca      240

gcagagggac acaggttcaa cccagccaag ctgctgatcg atccttgcgc ccgccagatc      300

gacggcgagt ttaaggataa tccactgctg cacgcaggac acaacgagcc tgactacagg      360

gataatgcag caatcgcacc aaagtgcgtg gtggtggtgg accactatga ttgggaggac      420

gatgcaccac ctaggacccc ttggggcagc acaatcatct acgaggccca cgtgaagggc      480

ctgacctatc tgcaccctga gatcccagtg gagatcaggg gcacatacaa ggccctggga      540

caccccgtga tgatcaacta tctgaagcag ctgggaatca ccgccctgga gctgctgcct      600

gtggcacagt tcgcatctga gccaaggctg cagaggatgg gactgagcaa ctactggggc      660

tataatcctg tggccatgtt cgcactgcac cctgcatacg catgtagccc agagacagcc      720

ctggacgagt ttagggatgc catcaaggcc ctgcacaagg ccggcatcga agtgatcctg      780

gacatcgtgc tgaatcactc cgccgagctg gacctggatg gccctctgtt ttccctgcgg      840

ggcatcgata acagatctta ctattggatc cgcgaggacg gcgattatca caattggacc      900

ggctgcggca acacactgaa tctgagccac ccagcagtgg tggactacgc atccgcctgc      960

ctgcggtatt gggtggagac ctgtcacgtg gacggcttca gatttgatct ggcagccgtg     1020

atgggaagga ccccagagtt ccgccaggac gcaccactgt ttacagccat ccagaactgt     1080

cctgtgctgt cccaggtgaa gctgatcgca gagccatggg atatcgcacc aggaggctac     1140

caggtcggaa acttcccacc cctgtttgcc gagtggaatg accacttccg ggatgccgcc     1200

cggagatttt ggctgcacta tgacctgcca ctgggagcct tcgccggcag atttgcagca     1260

agctccgacg tgttcaagag aaacggcagg ctgccctctg ccgccatcaa tctggtgacc     1320

gcccacgacg gcttcacact gagggattgc gtgtgcttta accacaagca caacgaggcc     1380

aatggcgagg agaatcgcga cggcaccaac aataactaca gcaataacca cggcaaggag     1440

ggactgggag gatccctgga cctggtggag aggcgccggg attctatcca cgccctgctg     1500

accacactgc tgctgagcca gggcacccca atgctgctgg caggcgatga gcacggacac     1560

tcccagcacg gcaataacaa tgcctactgc caggacaacc agctgacatg gctggattgg     1620

tctcaggcct ctagcggact gaccgccttc acagccgccc tgatccacct gagaaagagg     1680

atccccgccc tggtggagaa taggtggtgg gaggagggcg acggaaacgt gaggtggctg     1740

aatcgctatg cccagcctct gtctaccgac gagtggcaga acggcccaaa gcagctgcag     1800

atcctgctga gcgatagatt tctgatcgcc atcaatgcca ccctggaggt gacagagatc     1860

gtgctgccag caggagagtg gcacgcaatc cctccattcg ccggagagga caacccagtg     1920

atcacagccg tgtggcaggg accagcacac ggactgtgcg tgttccagag atga           1974


<210>  7
<211>  776
<212>  PRT
<213>  Pseudomonas amyloderamosa

<400>  7

Met Lys Cys Pro Lys Ile Leu Ala Ala Leu Leu Gly Cys Ala Val Leu 
1               5                   10                  15      


Ala Gly Val Pro Ala Met Pro Ala His Ala Ala Ile Asn Ser Met Ser 
            20                  25                  30          


Leu Gly Ala Ser Tyr Asp Ala Gln Gln Ala Asn Ile Thr Phe Arg Val 
        35                  40                  45              


Tyr Ser Ser Gln Ala Thr Arg Ile Val Leu Tyr Leu Tyr Ser Ala Gly 
    50                  55                  60                  


Tyr Gly Val Gln Glu Ser Ala Thr Tyr Thr Leu Ser Pro Ala Gly Ser 
65                  70                  75                  80  


Gly Val Trp Ala Val Thr Val Pro Val Ser Ser Ile Lys Ala Ala Gly 
                85                  90                  95      


Ile Thr Gly Ala Val Tyr Tyr Gly Tyr Arg Ala Trp Gly Pro Asn Trp 
            100                 105                 110         


Pro Tyr Ala Ser Asn Trp Gly Lys Gly Ser Gln Ala Gly Phe Val Ser 
        115                 120                 125             


Asp Val Asp Ala Asn Gly Asp Arg Phe Asn Pro Asn Lys Leu Leu Leu 
    130                 135                 140                 


Asp Pro Tyr Ala Gln Glu Val Ser Gln Asp Pro Leu Asn Pro Ser Asn 
145                 150                 155                 160 


Gln Asn Gly Asn Val Phe Ala Ser Gly Ala Ser Tyr Arg Thr Thr Asp 
                165                 170                 175     


Ser Gly Ile Tyr Ala Pro Lys Gly Val Val Leu Val Pro Ser Thr Gln 
            180                 185                 190         


Ser Thr Gly Thr Lys Pro Thr Arg Ala Gln Lys Asp Asp Val Ile Tyr 
        195                 200                 205             


Glu Val His Val Arg Gly Phe Thr Glu Gln Asp Thr Ser Ile Pro Ala 
    210                 215                 220                 


Gln Tyr Arg Gly Thr Tyr Tyr Gly Ala Gly Leu Lys Ala Ser Tyr Leu 
225                 230                 235                 240 


Ala Ser Leu Gly Val Thr Ala Val Glu Phe Leu Pro Val Gln Glu Thr 
                245                 250                 255     


Gln Asn Asp Ala Asn Asp Val Val Pro Asn Ser Asp Ala Asn Gln Asn 
            260                 265                 270         


Tyr Trp Gly Tyr Met Thr Glu Asn Tyr Phe Ser Pro Asp Arg Arg Tyr 
        275                 280                 285             


Ala Tyr Asn Lys Ala Ala Gly Gly Pro Thr Ala Glu Phe Gln Ala Met 
    290                 295                 300                 


Val Gln Ala Phe His Asn Ala Gly Ile Lys Val Tyr Met Asp Val Val 
305                 310                 315                 320 


Tyr Asn His Thr Ala Glu Gly Gly Thr Trp Thr Ser Ser Asp Pro Thr 
                325                 330                 335     


Thr Ala Thr Ile Tyr Ser Trp Arg Gly Leu Asp Asn Ala Thr Tyr Tyr 
            340                 345                 350         


Glu Leu Thr Ser Gly Asn Gln Tyr Phe Tyr Asp Asn Thr Gly Ile Gly 
        355                 360                 365             


Ala Asn Phe Asn Thr Tyr Asn Thr Val Ala Gln Asn Leu Ile Val Asp 
    370                 375                 380                 


Ser Leu Ala Tyr Trp Ala Asn Thr Met Gly Val Asp Gly Phe Arg Phe 
385                 390                 395                 400 


Asp Leu Ala Ser Val Leu Gly Asn Ser Cys Leu Asn Gly Ala Tyr Thr 
                405                 410                 415     


Ala Ser Ala Pro Asn Cys Pro Asn Gly Gly Tyr Asn Phe Asp Ala Ala 
            420                 425                 430         


Asp Ser Asn Val Ala Ile Asn Arg Ile Leu Arg Glu Phe Thr Val Arg 
        435                 440                 445             


Pro Ala Ala Gly Gly Ser Gly Leu Asp Leu Phe Ala Glu Pro Trp Ala 
    450                 455                 460                 


Ile Gly Gly Asn Ser Tyr Gln Leu Gly Gly Phe Pro Gln Gly Trp Ser 
465                 470                 475                 480 


Glu Trp Asn Gly Leu Phe Arg Asp Ser Leu Arg Gln Ala Gln Asn Glu 
                485                 490                 495     


Leu Gly Ser Met Thr Ile Tyr Val Thr Gln Asp Ala Asn Asp Phe Ser 
            500                 505                 510         


Gly Ser Ser Asn Leu Phe Gln Ser Ser Gly Arg Ser Pro Trp Asn Ser 
        515                 520                 525             


Ile Asn Phe Ile Asp Val His Asp Gly Met Thr Leu Lys Asp Val Tyr 
    530                 535                 540                 


Ser Cys Asn Gly Ala Asn Asn Ser Gln Ala Trp Pro Tyr Gly Pro Ser 
545                 550                 555                 560 


Asp Gly Gly Thr Ser Thr Asn Tyr Ser Trp Asp Gln Gly Met Ser Ala 
                565                 570                 575     


Gly Thr Gly Ala Ala Val Asp Gln Arg Arg Ala Ala Arg Thr Gly Met 
            580                 585                 590         


Ala Phe Glu Met Leu Ser Ala Gly Thr Pro Leu Met Gln Gly Gly Asp 
        595                 600                 605             


Glu Tyr Leu Arg Thr Leu Gln Cys Asn Asn Asn Ala Tyr Asn Leu Asp 
    610                 615                 620                 


Ser Ser Ala Asn Trp Leu Thr Tyr Ser Trp Thr Thr Asp Gln Ser Asn 
625                 630                 635                 640 


Phe Tyr Thr Phe Ala Gln Arg Leu Ile Ala Phe Arg Lys Ala His Pro 
                645                 650                 655     


Ala Leu Arg Pro Ser Ser Trp Tyr Ser Gly Ser Gln Leu Thr Trp Tyr 
            660                 665                 670         


Gln Pro Ser Gly Ala Val Ala Asp Ser Asn Tyr Trp Asn Asn Thr Ser 
        675                 680                 685             


Asn Tyr Ala Ile Ala Tyr Ala Ile Asn Gly Pro Ser Leu Gly Asp Ser 
    690                 695                 700                 


Asn Ser Ile Tyr Val Ala Tyr Asn Gly Trp Ser Ser Ser Val Thr Phe 
705                 710                 715                 720 


Thr Leu Pro Ala Pro Pro Ser Gly Thr Gln Trp Tyr Arg Val Thr Asp 
                725                 730                 735     


Thr Cys Asp Trp Asn Asp Gly Ala Ser Thr Phe Val Ala Pro Gly Ser 
            740                 745                 750         


Glu Thr Leu Ile Gly Gly Ala Gly Thr Thr Tyr Gly Gln Cys Gly Gln 
        755                 760                 765             


Ser Leu Leu Leu Leu Ile Ser Lys 
    770                 775     


<210>  8
<211>  2157
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon optimized polynucleotide coding sequence #2 for expressing 
       Pullulanase

<400>  8
atggttagca tcaggcgatc ctttgaagcc tacgtggatg acatgaatat catcaccgtg       60

ctcataccgg ccgaacaaaa ggagattatg acacctcctt tccgactcga aaccgagata      120

acagactttc ctctcgctgt tcgagaggaa tatagtttgg aagctaagta taagtacgtt      180

tgtgtaagcg atcatccggt tactttcgga aagatacatt gcgtaagagc ttcttctgga      240

cacaagaccg atcttcaaat aggtgccgta atacgcacgg cagcgttcga cgatgaattt      300

tattacgacg gtgaacttgg cgccgtatac actgccgacc atactgtttt taaagtttgg      360

gccccggctg caacttctgc ggctgttaaa ctttctcatc ccaataagtc aggacgaacc      420

ttccagatga ccagattgga gaaaggtgtg tacgcagtga ctgtcacggg ggatctccac      480

ggttatgaat acctgttctg catttgcaat aattctgaat ggatggaaac tgtcgatcaa      540

tacgccaagg ctgtgacagt caacggggaa aagggtgtgg ttctgcgccc ggatcaaatg      600

aagtggacag ctcctcttaa accattctca caccctgtgg acgcggtaat ttacgagacg      660

catctccgag atttcagtat tcacgagaat agtggtatga ttaataaggg gaagtacctg      720

gctttgacgg aaacagatac gcaaactgcc aacggatcaa gcagcggtct ggcatacgtc      780

aaggaactgg gcgtaacaca tgttgaactc ctccctgtca acgacttcgc tggtgttgac      840

gaggaaaagc ccttggacgc ttacaattgg ggatataatc cattgcattt ctttgccccc      900

gagggcagct acgcgagcaa ccctcatgat ccacagaccc gaaagactga gctgaaacag      960

atgattaaca cgctgcacca gcatggattg cgagtcatat tggacgtggt attcaaccat     1020

gtctataaac gcgagaacag tccctttgag aagacagtcc caggttactt cttcagacac     1080

gatgagtgtg gcatgcccag caacgggacc ggtgtcggta atgatatcgc gtccgaacgc     1140

cggatggccc ggaaatttat cgccgattgt gtagtttact ggcttgagga atataatgta     1200

gacgggtttc ggttcgattt gctcggtata ttggatattg acaccgttct ctatatgaag     1260

gagaaagcga cgaaggctaa gccgggcata ctcctgttcg gtgaaggttg ggatctggcg     1320

accccgctcc cgcacgagca aaaagcagct cttgctaacg caccgaggat gccgggaata     1380

ggatttttca atgacatgtt ccgcgatgcc gtgaaaggaa atacattcca ccttaaagcc     1440

acaggtttcg cactgggcaa cggtgagtcc gcccaagctg tcatgcacgg gattgcaggt     1500

tcatccgggt ggaaggccct tgcacctatt gtaccagagc cttctcaatc cattaattac     1560

gtcgaatcac atgataacca cacgttctgg gataaaatgt cattcgccct gccacaggaa     1620

aacgactcta ggaaacggtc ccgacaacgg ctggccgcag ctattatctt gcttgcacaa     1680

ggggtgccct ttatccattc cgggcaagag ttttttagaa ccaaacaagg agtcgagaac     1740

agctaccagt catcagactc catcaaccag ttggactggg ataggagaga gacgttcaaa     1800

gaggatgtgc attacatcag acggctcata tcactccgaa aggcacaccc ggcatttcgc     1860

ctcaggtctg ctgcggacat acagcgccat ttggagtgtc tcaccttgaa ggagcatctc     1920

attgcgtata ggttgtacga cttggacgag gtggatgagt ggaaggatat cattgttatt     1980

catcatgcgt ctcccgactc cgtcgagtgg cgattgccga acgacatacc ataccgattg     2040

ctgtgcgacc catcaggttt ccaggaggac ccaactgaaa tcaaaaaaac cgtggctgtc     2100

aatggtattg gtaccgttat actttacctc gcttcagatc tgaagtcttt cgcctga        2157


<210>  9
<211>  2157
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon optimized polynucleotide coding sequence #3 for expressing 
       Pullulanase

<400>  9
atggtctcca ttaggcgaag cttcgaggct tatgtagacg atatgaacat tattactgtc       60

cttataccag cagaacagaa agaaattatg actccgccgt tccgattgga gacagagatt      120

acggactttc ctttggccgt tcgggaagag tattctcttg aggccaaata taaatatgtg      180

tgcgttagtg atcatccagt aacgtttggc aaaatccact gcgtccgggc ctctagtgga      240

cataagacgg accttcagat aggggctgtc atccggacag ctgcattcga tgatgagttt      300

tactatgatg gggagctcgg agcggtatat acagccgatc atactgtatt taaagtttgg      360

gcacctgctg ccacctccgc ggcggttaag ctttcccatc cgaataaatc cggccgcact      420

tttcagatga ctcgacttga aaaaggggtt tacgccgtga cggtgaccgg agatttgcac      480

gggtacgagt atcttttctg catatgtaat aactcagaat ggatggaaac tgtagaccag      540

tatgccaagg cggttacggt gaacggggaa aagggggttg ttctgcggcc ggatcaaatg      600

aaatggacgg cacccttgaa gcccttttct catccagtcg acgctgtcat atacgagacg      660

cacctgagag acttttcaat ccacgaaaat agtggtatga ttaataaagg caaatatctt      720

gcacttacag agaccgatac acagaccgca aacggatcta gttctgggtt ggcgtacgtc      780

aaagagctgg gagttactca tgtagaattg ctccctgtta acgacttcgc aggtgtagat      840

gaggaaaagc ctctggacgc atataactgg ggatacaatc ctctccattt tttcgcacca      900

gaagggtcat atgccagcaa tccccatgac ccgcaaacga gaaagactga acttaagcag      960

atgataaata ctctgcacca acacggtctt cgcgttattc tcgatgttgt cttcaaccat     1020

gtttacaagc gcgagaattc ccctttcgaa aaaacggtac ctgggtactt ttttcggcat     1080

gacgaatgcg gtatgcctag caacggaaca ggagttggga acgacattgc aagcgaacgg     1140

cgaatggcga gaaagtttat tgccgattgt gtagtctact ggttggagga gtataacgtt     1200

gatggtttcc gattcgacct gttgggtata ttggatatcg ataccgtgtt gtacatgaaa     1260

gaaaaagcaa caaaagccaa acctgggatt ttgctgtttg gtgaaggttg ggacttggcc     1320

acgccgctcc cgcacgagca gaaggccgct ctcgcgaatg caccgcgcat gccaggaatc     1380

gggtttttta acgacatgtt cagggacgct gtgaaaggaa acacgtttca tcttaaggct     1440

acggggttcg ctcttgggaa cggcgagagc gcacaggcag tcatgcacgg tatagctggt     1500

agttcaggtt ggaaggcgct cgcaccaatc gtgcctgagc cgtcccagag cattaattat     1560

gtcgaatccc acgataatca caccttttgg gataaaatgt cctttgcctt gccacaggag     1620

aacgattcta gaaagcggtc acgccagcgg cttgctgctg caattatctt gttggcccag     1680

ggtgtcccgt tcatccacag tggccaggag ttcttcagaa caaaacaagg agtggagaac     1740

agctaccaaa gctcagactc tataaatcag ttggactggg acaggcgcga aacatttaaa     1800

gaagatgtgc actacattag gcgactgatt tcactccgca aagcgcaccc tgcattccga     1860

cttagatccg ccgctgacat acaaagacat ctggaatgcc tcactctcaa ggaacatctg     1920

atagcttata ggttgtacga cttggacgag gtcgacgaat ggaaagacat tatcgttata     1980

catcatgcgt ctcctgatag cgtcgagtgg agattgccaa acgatattcc atatcggctt     2040

ctttgcgacc cctctggctt tcaggaggac ccgaccgaga taaagaagac tgtggcggtt     2100

aacggtattg gtacggtaat tttgtacttg gcgtctgatt tgaaatcttt cgcgtga        2157


<210>  10
<211>  2157
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon optimized polynucleotide coding sequence #4 for expressing 
       Pullulanase

<400>  10
atggtaagta ttcggagatc ttttgaggct tacgttgatg atatgaacat tattacagtt       60

ctcattccgg cggagcaaaa ggagataatg accccaccat ttagacttga gactgagata      120

actgactttc ctcttgcagt tagggaagaa tatagtctgg aggcaaagta taaatacgta      180

tgcgtcagtg accaccctgt aacatttggt aaaatacact gtgtaagagc gtctagcgga      240

cataaaactg accttcagat tggcgccgtt atacggacag cagcattcga tgacgagttt      300

tattacgacg gggaacttgg ggcagtttac actgcagatc atacggtgtt taaagtttgg      360

gcgccagctg ctacctctgc agcagtaaaa ttgagtcacc ctaataaatc aggtaggacg      420

ttccagatga ctagactcga aaagggggtt tacgcagtca cggtaacggg tgatttgcac      480

ggctacgaat accttttttg catttgcaac aacagtgagt ggatggaaac cgttgaccaa      540

tatgccaagg ctgtgacggt caatggggaa aaaggtgtcg tgttgcggcc tgatcaaatg      600

aagtggacag cacccctcaa gccatttagt catcccgttg acgctgtaat atacgaaacg      660

cacttgcgcg acttctcaat tcacgaaaac tccggaatga taaacaaagg taaatacctt      720

gcacttactg aaacggatac ccagacggcg aacggaagta gtagcgggct cgcctacgtc      780

aaagaattgg gggttacaca cgttgaactc ctgcctgtta atgacttcgc cggcgtcgac      840

gaagagaagc cccttgatgc atataattgg gggtataacc ccctgcattt ctttgcccct      900

gagggatcat atgcaagtaa tccgcatgat ccacagactc gaaaaacaga gctcaaacaa      960

atgattaaca cgcttcacca gcatggtctt agggtgatcc ttgacgtggt ttttaaccat     1020

gtgtataagc gagagaactc ccctttcgaa aagactgtcc ccggatattt ctttcgccat     1080

gatgagtgcg gtatgcctag caatgggact ggggtcggta atgatatcgc cagcgaaagg     1140

cgaatggcta gaaaatttat agcggactgc gtagtatact ggctcgagga atataacgtt     1200

gacggatttc gcttcgattt gttgggaatc cttgacatag acactgtact ttatatgaag     1260

gaaaaggcta caaaagcaaa gcccggaata ttgctctttg gcgaggggtg ggatcttgcg     1320

acgcccctcc cccacgaaca gaaagccgct ctcgcaaacg ctccccgaat gcctggcatt     1380

ggatttttca atgatatgtt ccgagacgcc gtaaagggca acaccttcca tctgaaagcc     1440

actggatttg ccctcggtaa cggagaatcc gctcaagctg tcatgcacgg tattgcaggc     1500

agcagcgggt ggaaggcctt ggcccccata gtgccggaac cctcacagtc aatcaactat     1560

gtggaaagtc atgataacca tactttttgg gacaagatgt catttgcatt gccgcaagag     1620

aacgactcca ggaagcggag ccggcaaagg ttggcggccg cgatcatcct gttggctcag     1680

ggagtgccct tcatccattc cggtcaggag ttctttcgga ccaaacaggg ggttgaaaac     1740

tcctaccaat catctgattc cataaatcaa ctcgactggg atagacgaga aaccttcaaa     1800

gaggacgtcc actatataag gagactgata tctttgcgaa aggcgcatcc tgcttttcgg     1860

ctccggagcg ctgcggacat ccagagacat ctcgagtgtc tgacccttaa ggagcatttg     1920

attgcctatc gactgtatga cttggatgag gtcgacgaat ggaaggatat catagttatt     1980

catcacgcct ctccagacag tgttgaatgg cgactgccta acgacatccc ctacaggctg     2040

ctctgcgacc cgtccggttt tcaggaagac ccgacagaaa ttaaaaagac cgtggccgtg     2100

aatggaattg ggacagtcat cctctacctg gcatcagatc tcaagtcttt tgcctga        2157


<210>  11
<211>  2157
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon optimized polynucleotide coding sequence #5 for expressing 
       Pullulanase

<400>  11
atggtatcta tccggaggtc atttgaggcc tacgttgacg atatgaacat tattacagtt       60

ctgataccgg ctgaacagaa agaaattatg acaccacctt tccgactgga gactgaaata      120

accgacttcc ccctggcggt gagagaagag tacagcctgg aggctaagta caagtatgta      180

tgtgtaagcg atcatccagt gacctttggc aagatccatt gtgttcgagc ttcatccggg      240

cacaagaccg atctccagat tggcgccgtc atccgaacgg cggcgttcga cgatgagttc      300

tattacgacg gggaactggg ggcagtttac accgctgatc atactgtctt taaggtctgg      360

gcccccgcgg ctacctctgc cgcagttaag ctttcacacc cgaacaagag cgggagaaca      420

tttcaaatga cgaggttgga gaagggcgtc tatgcagtca cggtcactgg agatctccac      480

ggctatgaat acctcttttg catatgtaat aacagtgagt ggatggagac agtagaccag      540

tacgcgaaag ccgttacagt caacggggag aaaggagtgg tactgcgccc cgaccagatg      600

aaatggaccg caccccttaa gcccttcagc caccctgttg acgcggtgat ctatgagacc      660

caccttagag attttagcat acatgaaaac agcggcatga ttaataaagg taagtacctt      720

gccctgaccg agacagacac tcagacggcg aacggaagct cctcaggtct cgcatatgtg      780

aaggaactcg gcgtgaccca tgtcgagctc ctccctgtta acgatttcgc aggggtcgac      840

gaggaaaaac cgttggatgc gtacaattgg ggctacaacc cccttcattt tttcgctccc      900

gaaggaagtt atgcaagcaa tccacacgat ccccaaacaa ggaaaactga actcaagcag      960

atgattaata cactgcatca acacgggctg agagttatcc tggatgtggt ttttaaccat     1020

gtttacaaga gagaaaacag ccccttcgag aaaactgttc cgggttactt ttttcggcat     1080

gacgagtgtg gcatgccttc aaacggaact ggtgtgggta atgatatagc ttccgagcga     1140

cgaatggcca ggaagtttat agctgactgt gtcgtgtact ggctcgagga gtataatgtt     1200

gatggttttc gattcgattt gctgggcatc cttgacatcg atacggtcct ctatatgaaa     1260

gaaaaggcga cgaaggcgaa acctggaatt ctcttgtttg gtgagggttg ggaccttgca     1320

acgccgctgc cccatgaaca aaaggcagcc cttgcgaatg cgcccaggat gccggggatt     1380

ggtttcttta atgatatgtt tcgggatgcc gtgaagggga acacgtttca ccttaaggct     1440

acgggatttg ccttgggaaa tggtgaatca gcacaggcag tgatgcatgg cattgccggc     1500

agctccggtt ggaaagcact ggcgccgatt gtgcctgaac cctcacaatc tattaattac     1560

gtagaatcac atgacaatca cactttctgg gacaaaatga gcttcgcatt gccccaggag     1620

aacgatagca ggaaaaggtc acgccagagg ctcgcagcag cgatcatttt gcttgcccag     1680

ggcgttccat ttatacattc aggacaagag ttcttccgga cgaaacaggg tgtcgagaat     1740

tcctatcaga gtagtgatag tataaatcaa ctggattggg accgccgaga gacattcaag     1800

gaagacgtac actacatcag acgccttatc tctctgcgca aggcacaccc agcatttcga     1860

ttgagatcag cggccgacat ccagagacac ctggagtgtc tgacactgaa agaacatttg     1920

atagcctatc gcctctatga tcttgacgag gtcgatgagt ggaaagatat tatcgtcata     1980

catcatgcat cacccgacag cgttgaatgg cggcttccga acgacattcc ttatcggctg     2040

ttgtgcgatc cgagtgggtt ccaggaagat ccaacggaga taaaaaaaac agtggcagtc     2100

aatggcatcg gtacggtcat tctctacctg gcttcagatc tcaagtcatt tgcgtga        2157


<210>  12
<211>  2157
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon optimized polynucleotide coding sequence #6 for expressing 
       Pullulanase

<400>  12
atggtgagta tccgcaggag ctttgaagct tatgtagatg acatgaacat tattaccgtt       60

ctcatccctg cagaacaaaa ggaaataatg acgcctccct ttcgccttga gacagaaatt      120

acagactttc ctctggcagt gcgagaagag tattctctgg aggctaaata taagtacgta      180

tgcgtgagcg accaccctgt tacgttcggc aaaatacatt gtgtgcgcgc ttcctctggc      240

cataagacag atctccagat cggtgctgta attagaaccg ccgccttcga cgacgaattt      300

tattacgatg gagagcttgg ggctgtgtat actgctgatc acaccgtgtt taaagtgtgg      360

gccccggcag caactagtgc ggcagtcaaa ctctcacacc caaacaaatc tggtagaact      420

tttcaaatga ctaggctgga gaaaggtgtg tacgcagtca ctgttaccgg agatttgcat      480

ggttacgaat acctgttttg tatctgcaac aatagtgagt ggatggagac cgtagaccaa      540

tacgccaaag ctgttactgt caatggcgaa aagggggtcg ttcttcggcc ggatcagatg      600

aagtggacag ctccattgaa gcccttcagc caccctgttg acgccgttat ctatgaaaca      660

cacctgcgag acttcagtat tcatgaaaat tctggaatga taaacaaggg gaagtatctt      720

gctctcactg aaactgatac ccagactgcc aacggcagta gtagcggcct cgcctacgta      780

aaggagctcg gagttactca tgttgaactt cttccggtaa atgatttcgc aggtgttgat      840

gaagagaagc cattggacgc atataattgg ggttataacc cgcttcactt tttcgctccc      900

gaagggtctt atgcctctaa cccccatgat ccacagacac gcaagactga gcttaaacag      960

atgattaata cactgcacca acatggtctt agggtgattt tggatgtggt atttaaccac     1020

gtatacaaac gagaaaactc cccttttgag aaaaccgttc ccggttactt tttccggcat     1080

gatgagtgcg gcatgcctag taatggtacc ggggttggaa atgacattgc gtcagaacga     1140

cgaatggccc gcaagttcat tgctgattgt gtcgtatatt ggctcgaaga gtataacgtc     1200

gacgggtttc gctttgactt gcttggtatc ctcgatattg ataccgtcct ctacatgaag     1260

gaaaaagcga caaaagcaaa accgggaata ctcctcttcg gcgagggctg ggacctggcg     1320

acaccattgc ctcacgagca gaaggctgcg ctcgcgaatg cgccacgcat gcctggtata     1380

gggtttttta acgatatgtt tagggacgcg gtaaaaggca acacgttcca tctcaaggct     1440

acaggattcg cactcgggaa tggagaatca gctcaggctg tcatgcatgg cattgcaggc     1500

tcatccggtt ggaaagcttt ggcaccgatt gtacctgagc catctcagtc tatcaattac     1560

gtggagtcac atgataatca cacgttttgg gataagatga gttttgccct cccgcaagag     1620

aatgactctc gaaaaagaag tcgacaaaga ctggccgcgg ctataattct cctcgcgcag     1680

ggcgttccgt ttatacactc tggacaagaa ttttttagga ctaagcaagg ggtagaaaat     1740

agttatcagt ctagcgattc tattaaccag ttggactggg acagaaggga gacgttcaaa     1800

gaagatgtgc actacatacg aaggcttata agcttgcgca aggctcaccc agcttttaga     1860

cttcgatccg cagcggatat tcaacggcac ttggaatgtc ttacgctgaa ggaacacctc     1920

atagcctata gactgtatga cttggacgaa gttgacgagt ggaaagatat tattgtgata     1980

caccatgcgt cacctgacag tgtagagtgg agattgccta acgacattcc ctaccgcctg     2040

ctctgtgatc cctctgggtt ccaagaggac ccgaccgaaa tcaagaaaac agtagcagtt     2100

aacggaatcg gtactgtaat actttatctc gcttccgatc tgaagtcctt cgcatga        2157


<210>  13
<211>  2157
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon optimized polynucleotide coding sequence #7 for expressing 
       Pullulanase

<400>  13
atggtatcca taaggagaag cttcgaagct tatgtggatg atatgaatat aataaccgta       60

ctgattcccg cggaacaaaa agaaataatg acgccgccct tcaggttgga gacggaaata      120

acggatttcc cgttggcagt acgagaggaa tatagcctgg aggcgaaata taaatatgta      180

tgcgtgagcg accatcccgt gacatttggc aaaatacatt gcgtaagggc ttcctccgga      240

cataagactg accttcaaat aggtgcagtc atacgaacgg ccgcatttga tgatgagttt      300

tattatgacg gcgagttggg cgcggtctat actgctgacc ataccgtgtt taaagtctgg      360

gctcctgcgg ctacatcagc cgcggtcaag ctctcccacc caaataagag tgggcgaaca      420

ttccaaatga cgcgcctcga gaagggtgtt tacgcggtta cggtcaccgg ggatttgcat      480

gggtacgaat acctgttctg catatgtaac aacagtgagt ggatggaaac ggtggatcaa      540

tacgcgaaag cggtgactgt taacggagaa aagggcgtag tccttagacc cgaccaaatg      600

aagtggactg cacctctgaa accattctca cacccagtgg atgctgttat ttatgaaact      660

catcttaggg actttagcat ccatgagaac tccggaatga tcaataaggg aaagtacctg      720

gcacttacgg aaaccgatac ccagacggcc aatggatcta gttcagggct cgcttatgtc      780

aaggagttgg gtgtcacgca cgtagagctt ctccccgtca atgactttgc gggggtggac      840

gaggaaaaac cccttgatgc gtacaactgg ggctacaacc ccctgcattt cttcgccccc      900

gagggcagtt acgcgtctaa tccacatgac ccacaaacac gaaagaccga gctcaagcag      960

atgataaaca cacttcacca gcacgggctc agggtaattc ttgatgtagt gtttaaccac     1020

gtgtacaaga gggagaactc accttttgaa aagaccgtac cgggatattt ttttagacat     1080

gacgaatgcg gaatgccgtc caacggaaca ggcgttggca atgacatagc tagcgagcgg     1140

cggatggcac gaaagttcat agcagattgt gtcgtttact ggcttgagga gtacaacgtg     1200

gacggattta ggtttgacct tctcggaatt ctggatattg atacggttct ttacatgaaa     1260

gagaaagcga caaaggctaa gccaggtata ttgctctttg gtgaaggatg ggaccttgca     1320

acaccactgc cccatgaaca gaaagcagcc ctcgccaatg cgccccggat gcctggcatt     1380

ggcttcttca atgatatgtt tcgggacgca gtaaagggca acacctttca ccttaaagcc     1440

acaggatttg cactcgggaa tggtgaaagc gcgcaggccg ttatgcacgg cattgccggt     1500

agttctggtt ggaaggccct tgctccaatt gtgcctgaac cgagccaatc tataaactac     1560

gtcgagagtc acgataacca cactttttgg gataaaatga gtttcgcgtt gccgcaggag     1620

aatgacagca ggaaacggag taggcagcgc cttgccgcag caattatatt gttggcccaa     1680

ggtgtgcctt ttatacactc agggcaagag ttctttcgaa ctaaacaagg tgtcgagaac     1740

tcttaccagt cctccgattc catcaaccag ctggattggg accgccgcga gacttttaaa     1800

gaagacgttc actacatcag gagacttatt agtctccgca aagctcatcc agcgttcagg     1860

cttcggtccg ccgctgacat ccaacgacac cttgagtgtc tcacactcaa ggagcatctg     1920

atcgcttacc gcttgtatga cttggatgaa gtagacgagt ggaaagacat aatcgtaatc     1980

caccatgcct caccagattc tgtcgagtgg aggctcccca acgatattcc ctatcgcttg     2040

ctgtgcgatc cgagcggttt ccaagaggac ccaaccgaaa taaaaaaaac cgttgcggta     2100

aacggtatcg ggactgttat actttacctg gctagcgatc tgaaatcttt tgcctga        2157


<210>  14
<211>  2157
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon optimized polynucleotide coding sequence #8 for expressing 
       Pullulanase

<400>  14
atggtgtcca taaggcggag cttcgaggct tacgtagacg atatgaatat tattactgtt       60

ttgatccccg ccgaacaaaa agagattatg actcccccct ttagattgga gacagagatc      120

acggatttcc cattggcggt acgggaggag tactctttgg aagcaaagta taagtatgtc      180

tgtgtaagtg atcatccggt tactttcggc aagatccact gcgttagagc gtcatccggt      240

cacaaaaccg acctgcagat tggagcggtg attagaacag cggctttcga tgacgaattt      300

tactacgacg gtgagctcgg cgctgtgtac acagccgacc acacagtatt taaagtatgg      360

gctcccgcag ctacaagcgc agcagtaaag ctctcccatc caaataaaag tggcagaacg      420

tttcagatga ctcgactcga gaagggagtc tacgccgtta ccgtcacggg ggatctccac      480

gggtatgagt atctgttttg catttgcaac aactctgaat ggatggaaac ggtggatcag      540

tacgctaagg cagtgaccgt taatggcgaa aagggcgttg tgctgagacc cgatcagatg      600

aaatggacgg cccctcttaa accattctct catcctgtgg acgccgtcat ttatgaaacg      660

cacttgagag atttcagtat acacgaaaat agtggaatga taaacaaggg aaagtacctg      720

gcacttaccg aaactgatac gcagaccgca aatggaagtt catctggact cgcctatgtc      780

aaagagcttg gtgttactca tgttgagttg ctgcctgtca acgacttcgc tggagtagac      840

gaggaaaagc ctcttgacgc atataactgg ggttacaacc cgctgcactt cttcgccccc      900

gaaggatcct acgctagcaa tcctcacgac ccgcaaaccc gcaagacaga actcaaacag      960

atgataaaca cccttcatca gcatggcctt agagtgattc ttgacgttgt cttcaatcat     1020

gtttacaaac gagagaatag tcctttcgaa aaaactgtcc cgggatattt ctttcggcac     1080

gatgagtgcg gaatgccgtc aaacggaacg ggcgttggga acgatatagc ttccgagcga     1140

agaatggccc ggaagttcat tgctgattgc gtcgtctatt ggctggagga atacaatgtg     1200

gacgggtttc gctttgacct gctcggaatt ttggatatag acacagtact gtatatgaaa     1260

gagaaggcga ccaaggcgaa acccggcatt cttcttttcg gtgaagggtg ggaccttgcc     1320

acacccctgc cccatgaaca aaaggccgcc ctggctaacg cccccagaat gccgggaata     1380

ggctttttca atgatatgtt ccgagatgcg gtaaaaggta atacatttca ccttaaggca     1440

acagggttcg cgctcgggaa tggcgaatca gcgcaggccg tgatgcacgg tatcgctggt     1500

tcttcaggat ggaaggctct cgctccgatc gtaccggagc cgtctcagtc aattaattac     1560

gtggagagtc acgataatca tactttttgg gataagatgt ctttcgcact cccccaggaa     1620

aacgactccc gaaaaagatc aaggcaacgg ttggcagctg ctataatctt gctcgcccaa     1680

ggtgttccat ttatccattc tggtcaagaa ttctttcgaa cgaagcaggg tgtggagaat     1740

agctaccagt cctccgactc aatcaatcaa cttgattggg atagaaggga aacttttaag     1800

gaagatgttc attacatccg ccggctgata tctttgagaa aggcacaccc agctttccgc     1860

ctgaggagcg cggcggacat acagcgccat ttggagtgcc tgacactcaa agagcacctc     1920

attgcctata gactctacga tttggacgaa gtagacgagt ggaaggacat tatagtaata     1980

caccatgcgt ctcccgacag cgtagaatgg cgcctcccta acgacatacc ttatcggctc     2040

ctctgcgacc ccagtggatt tcaagaggat ccgaccgaga tcaagaaaac agtcgcggta     2100

aacggtatag gtacggtcat actgtatctc gcctcagact tgaagagctt tgcgtga        2157


<210>  15
<211>  2157
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon optimized polynucleotide coding sequence #9 for expressing 
       Pullulanase

<400>  15
atggtaagca tccgccgatc attcgaggct tacgtggacg acatgaacat tattaccgtt       60

ctcatccctg ctgagcaaaa ggaaatcatg acgccaccct tccgccttga gacagaaata      120

acagattttc ccctggcggt gagagaggag tactccctgg aagccaaata caaatacgtg      180

tgtgttagcg accatccagt aacgttcggg aagatccatt gcgtgcgggc atcctccggc      240

cacaaaacgg atcttcagat tggagccgtg attcggacag ctgcgtttga tgatgagttt      300

tactacgatg gagaactggg ggcagtatat acggccgacc atacggtatt taaggtgtgg      360

gcccctgcag ctacatcagc ggctgtcaaa cttagccatc caaacaagtc cgggcgaacg      420

tttcaaatga ctagactgga gaaaggggtg tatgctgtca ccgttactgg ggaccttcac      480

ggatacgagt acttgttttg catatgtaat aattccgagt ggatggagac cgtagaccag      540

tatgctaaag ctgtaactgt taatggtgaa aagggtgttg ttctccggcc agaccaaatg      600

aaatggaccg cgccactcaa acccttttct catccagtgg atgcagtgat ttatgagacc      660

cacttgcgag atttctcaat acatgaaaac tctggtatga tcaacaaggg caaatatttg      720

gcactcacgg agaccgatac gcaaaccgct aatgggagta gtagtggcct cgcctacgta      780

aaagagctcg gggtaacgca tgtagagctc cttccagtca atgactttgc aggggtagat      840

gaagaaaaac ctctggatgc gtacaactgg ggctacaatc cacttcactt cttcgctcct      900

gagggttctt atgcaagcaa cccgcacgac cctcaaacac ggaaaactga acttaaacaa      960

atgattaata cactccatca gcatggtctg cgcgtcatct tggatgtggt cttcaatcac     1020

gtttataaac gagaaaacag tccgtttgag aaaaccgttc ctggatattt tttcaggcat     1080

gatgaatgcg gcatgccctc taatggtacg ggagttggta acgacatagc tagtgaaaga     1140

cgaatggcgc gcaaattcat agcagactgc gtggtctatt ggctggagga atataacgtt     1200

gatggcttca gatttgacct gctcggcatt ctcgacattg acacggttct ttacatgaag     1260

gagaaagcaa cgaaagccaa accgggaatc cttttgttcg gcgagggttg ggatcttgct     1320

acccctttgc cacatgagca aaaggctgct ctggcaaacg ctcctagaat gcctggaata     1380

ggcttcttta atgatatgtt tagggacgcc gtcaagggga acacgtttca cctgaaagca     1440

acaggatttg cgctgggaaa cggcgaaagc gctcaagctg taatgcatgg gatagcgggc     1500

tcctccggat ggaaagccct ggcgcctatt gtacccgagc cgtcacaaag tatcaattac     1560

gtggaatctc atgataatca tactttttgg gacaagatgt cattcgcgct gcctcaagag     1620

aacgactctc ggaagagatc tcggcagcgc cttgcagccg caatcattct tttggctcag     1680

ggagtcccct tcatccatag tgggcaagaa tttttcagaa ctaagcaagg cgtggaaaat     1740

tcctaccaaa gctccgattc cataaatcaa ctggactggg atcgccgaga gaccttcaaa     1800

gaggatgttc actacattcg gaggttgatc tctttgagaa aagcccaccc ggccttcagg     1860

ctgaggagtg ccgcagacat tcaaagacac ctcgaatgtc ttactctcaa ggagcatctt     1920

attgcgtacc ggctgtacga cttggacgag gtggacgagt ggaaagatat aattgttata     1980

catcatgctt ccccggatag tgtcgagtgg cgcttgccca acgatatccc ctacagactc     2040

ttgtgtgacc caagcggctt tcaagaggat ccaacggaaa tcaaaaaaac tgtagcagtc     2100

aacggcattg gtacggttat cctttacctc gcgtccgacc tcaagagctt tgcgtga        2157


<210>  16
<211>  2157
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon optimized polynucleotide coding sequence #10 for expressing
       Pullulanase

<400>  16
atggttagca ttaggcggag cttcgaggct tatgtagatg atatgaatat cataaccgtt       60

ctcatccctg cagagcaaaa agaaataatg acacctccct ttaggcttga aacggaaatc      120

acggatttcc cccttgcggt ccgcgaagaa tactccttgg aagccaaata caagtacgtc      180

tgtgtctctg accaccctgt aacgtttggt aaaattcact gcgtacgcgc gagctcagga      240

cataagacgg accttcagat aggggcagtt attcggacag ctgcttttga cgacgagttt      300

tattacgacg gtgaattggg cgcagtttac actgccgatc acactgtttt caaggtgtgg      360

gcacctgctg ccacatctgc cgcggttaag ttgtctcacc ctaacaagag cggccggaca      420

tttcagatga cccgactcga aaagggggta tacgcagtga ccgtaactgg tgatttgcac      480

gggtacgaat accttttctg catttgtaac aattccgaat ggatggagac agtggaccag      540

tatgcaaagg cagtgacggt caacggagaa aagggcgttg tgttgcgccc tgatcagatg      600

aagtggacgg cccctcttaa accttttagt cacccagtag atgcagtgat atacgagacg      660

catctgagag acttctctat acatgagaat tcaggtatga ttaataaggg taagtacctg      720

gcccttaccg agacagatac ccaaactgca aatggctcct ctagtggcct ggcatatgta      780

aaggaactgg gagtgaccca tgtcgaactc ctcccagtaa atgattttgc gggggttgat      840

gaggaaaagc cacttgatgc atacaattgg ggttacaacc cactgcactt ttttgctcct      900

gaagggtcct acgctagcaa ccctcatgat cctcaaacca ggaagacaga attgaaacaa      960

atgattaata ccctccacca gcacggtttg agagtgatcc tcgatgttgt ctttaaccat     1020

gtgtacaaac gggagaacag tcccttcgaa aagacagtcc cggggtactt tttccgccac     1080

gatgaatgtg gcatgccctc caatggaact ggtgtaggca atgacattgc gtctgaaaga     1140

aggatggcca ggaagtttat agctgattgt gtagtctact ggttggagga atacaatgtg     1200

gacggttttc ggtttgatct gctcgggatc ctggacatcg atactgtatt gtacatgaag     1260

gagaaagcta ctaaagcaaa accgggcatc ttgttgtttg gtgaaggatg ggatcttgca     1320

acgcctcttc ctcatgaaca gaaagcagcg ttggcaaacg caccaaggat gccggggata     1380

ggtttcttca atgatatgtt ccgggatgcc gtcaaaggaa acacattcca cctgaaggcc     1440

acaggcttcg ctctcggcaa cggcgagtca gcgcaagccg taatgcatgg catcgcaggc     1500

tcctcagggt ggaaagcact ggcgcctatt gtgcctgaac catctcaaag cataaactac     1560

gtcgagtccc acgacaacca tactttttgg gataaaatga gctttgcgct cccacaagaa     1620

aacgacagtc ggaaacggag caggcagcga ctcgctgccg ctattatact cttggctcag     1680

ggggttccct ttatacacag cgggcaagaa ttctttagaa ccaagcaggg agtagaaaat     1740

tcttatcaga gttccgactc aatcaaccaa ctcgattggg ataggcggga aacatttaag     1800

gaggacgtcc attacataag gagacttatt tctctcagaa aagcacatcc tgctttcagg     1860

ttgcgatccg ccgccgatat tcaacgccat ctcgagtgcc tcacactgaa ggaacacctc     1920

atcgcatacc gactctacga tttggatgag gtggatgagt ggaaagacat aatcgtcata     1980

caccatgcta gcccggattc cgtagagtgg cggttgccga atgacatccc ataccgactg     2040

ctttgtgatc cttcaggttt ccaagaagac cccaccgaga ttaagaaaac agtggccgtt     2100

aatggtattg gaacggtcat tctgtacctc gcatctgacc tgaaatcatt cgcgtga        2157


<210>  17
<211>  1974
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon optimized polynucleotide coding sequence (#2) for 
       expressing the bacterial limit dextrin alpha-1,6-glucohydrolase 
       (GlgX)

<400>  17
atgacccagt tggcaatagg aaagcccgca ccacttggcg cacattatga tggtcagggt       60

gttaatttca ctcttttttc cgctcatgcg gagagggtag aactctgtgt cttcgacgca      120

aatggccaag aacatagata tgacttgccg ggacactcag gcgacatttg gcatgggtac      180

ttgcccgatg ctcggcctgg cctcaggtat ggatatcgcg tacacggacc gtggcaaccg      240

gcagaagggc atcggttcaa tccagcaaaa ctcctcatag acccgtgcgc aaggcagatt      300

gatggagagt tcaaagataa cccgcttttg catgcgggcc ataacgaacc tgactatcgg      360

gataacgcgg caatcgcgcc gaaatgtgta gtagttgttg accattacga ttgggaagat      420

gatgcaccac cgcgaacacc ttggggtagc accattattt atgaagcaca tgtcaaaggt      480

cttacgtacc tgcatccgga gattcccgtg gaaataagag gaacgtacaa agccttgggt      540

caccccgtaa tgataaacta ccttaaacag ttgggaataa ccgcattgga attgctgccc      600

gtggcgcaat ttgccagcga gccgcgattg caacggatgg gtctcagtaa ttactggggg      660

tataatccgg tagctatgtt cgcccttcac cctgcttatg catgttctcc agagactgca      720

ttggacgaat tccgcgacgc aataaaagca cttcacaagg ctggaattga ggttattttg      780

gacattgtgt tgaaccactc tgccgagctt gacttggatg ggccgctgtt ttcactgagg      840

ggtatcgaca acaggagcta ctattggatc cgggaagatg gcgactacca caactggaca      900

ggatgtggga atactctgaa tctctctcac ccggctgtcg tggattatgc cagcgcttgc      960

ctgcgatatt gggtcgagac gtgtcacgtt gatgggttcc gatttgactt ggctgcagtg     1020

atggggagaa cgccagagtt ccgacaggat gccccccttt tcaccgctat tcaaaattgc     1080

cccgtactga gtcaggtgaa gctcatcgct gagccgtggg acatagcacc gggcggatac     1140

caagtgggaa attttccgcc gttgtttgct gaatggaatg accatttccg ggacgcagct     1200

cgccgatttt ggttgcacta tgatctccct ttgggggctt tcgctggccg ctttgctgct     1260

tcttcagatg ttttcaaaag aaacggacgg ctgccatccg ccgcaataaa cctcgtaaca     1320

gcccacgacg gatttacact tcgggactgt gtttgtttta accataagca caatgaagca     1380

aacggggagg aaaaccgaga cggcactaac aataactata gtaacaacca cggcaaagag     1440

ggcttgggtg gaagtctgga tctggtggag cggcggcgcg actccattca tgctctgctt     1500

accactcttt tgctgtccca gggtacccca atgctcctgg ctggggatga acatggccac     1560

tcccagcatg gcaataacaa tgcatattgt caggacaatc agcttacttg gctcgactgg     1620

agccaagcgt catcaggcct cacagcattt accgctgccc ttattcactt gcgcaaacgc     1680

attcctgctt tggtcgaaaa ccgatggtgg gaagagggag atggcaatgt gaggtggttg     1740

aatcggtacg cccagccgct tagcactgat gagtggcaga atggcccgaa gcagctccag     1800

atactccttt cagatcgctt tttgatagcg atcaacgcca cacttgaggt gaccgagatt     1860

gttctccctg caggggaatg gcacgcaatc cctccgttcg caggtgagga taaccccgtt     1920

ataacagctg tttggcaagg ccccgcccat gggctgtgcg tattccaacg atga           1974


<210>  18
<211>  1974
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon optimized polynucleotide coding sequence (#3) for 
       expressing the bacterial limit dextrin alpha-1,6-glucohydrolase 
       (GlgX)

<400>  18
atgacccaac ttgcgatcgg caagccggca ccgctggggg cacactacga cggccagggt       60

gttaacttca cgctgttctc agcacatgca gaacgcgtag agctctgtgt attcgacgct      120

aatggacaag agcacaggta tgatctgcct gggcactccg gagacatctg gcatggttat      180

ttgccagacg ctcgccccgg actcaggtac gggtaccgag tgcacggacc atggcaacca      240

gccgaaggac accgctttaa cccagccaag ctgctgattg acccgtgcgc gaggcaaatc      300

gacggagaat ttaaagataa ccctcttctt cacgcaggac acaatgaacc cgactatcga      360

gataatgctg cgattgcacc aaagtgcgtc gtcgtcgtcg accattatga ctgggaagac      420

gatgcgccac cccgaacacc atgggggagc acgataatat atgaggctca cgttaagggc      480

ctgacgtact tgcatccgga gatacctgta gaaatcaggg ggacctacaa ggcccttggt      540

catcccgtta tgattaacta tttgaagcag cttggaatta ctgcccttga acttctcccc      600

gtggcgcaat ttgcatcaga accccggctg cagcgaatgg gactgagtaa ctactgggga      660

tacaatccag tggccatgtt tgcactccac cctgcttatg cgtgtagccc cgaaacagca      720

ttggacgagt tcagggatgc aatcaaggcc ttgcataaag ccgggattga ggtaatactc      780

gacatagttc tgaatcatag cgcggaactg gatctcgatg gaccactttt ttcacttaga      840

gggattgaca accgctcata ttattggatc agagaggatg gcgattatca taattggaca      900

gggtgtggaa acacactcaa tctctcccat ccggctgtgg tagattatgc atccgcttgc      960

ctccggtatt gggtagaaac atgtcatgtg gacgggttcc ggttcgatct ggcggccgtc     1020

atgggcagga cacctgaatt cagacaggac gcccctttgt tcaccgccat acagaactgt     1080

ccggtcttgt cccaagtgaa gttgattgcg gaaccatggg acattgcccc tgggggctac     1140

caggtcggaa attttccgcc cctcttcgct gagtggaatg accacttccg ggatgcagcc     1200

cgccgctttt ggttgcacta cgatttgccg ttgggcgcct tcgctgggcg ctttgctgcg     1260

agctcagacg tatttaaaag aaatgggcga ttgcctagtg cggcaattaa tctggtgact     1320

gcacacgatg gatttacgct tagggactgt gtttgtttta atcacaagca taatgaagcg     1380

aacggggaag aaaacaggga cggaactaat aataattatt caaacaatca tggtaaggaa     1440

ggtttggggg gttcccttga tcttgttgaa agacgaaggg actccatcca cgcccttctc     1500

actactcttc tgcttagtca aggtacgcct atgttgctcg ccggcgatga acacggacac     1560

tcccagcatg gcaataacaa cgcatactgc caggataatc agctgacctg gctcgattgg     1620

tcccaggcct cctccggatt gaccgccttt accgctgcgt tgatacacct tcgcaaacga     1680

attcccgcat tggtggagaa taggtggtgg gaagagggcg acggcaacgt gaggtggctc     1740

aacaggtatg ctcaaccgct ttccaccgat gagtggcaaa acgggcccaa acagctccaa     1800

attctgctga gcgaccgatt cttgattgca atcaatgcga ccctcgaggt caccgaaatc     1860

gtcttgcccg ctggtgagtg gcacgctata ccgccatttg cgggtgagga caatcccgta     1920

ataacagcgg tctggcaggg accagcacac ggtctctgtg tgttccaacg atga           1974


<210>  19
<211>  1974
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon optimized polynucleotide coding sequence (#4) for 
       expressing the bacterial limit dextrin alpha-1,6-glucohydrolase 
       (GlgX)

<400>  19
atgacgcagc tcgcaatagg aaagccagca ccccttggag cccattatga tggtcagggc       60

gttaatttca cactgttttc tgcccatgca gaacgggtcg aattgtgtgt atttgacgct      120

aatggtcaag agcatcgcta tgacctcccg ggacacagtg gggacatttg gcatggctat      180

ctgcctgacg cgcgcccagg gctccggtac gggtaccgag ttcacggtcc ctggcaacca      240

gccgaggggc ataggtttaa tccggcaaag ctgctcattg acccctgcgc tcgccagata      300

gacggagaat ttaaggacaa ccccctgctc cacgctggtc ataatgaacc agactatcga      360

gacaatgcgg ccattgctcc gaaatgtgta gtcgttgttg atcactatga ctgggaagac      420

gacgctccac ctagaactcc ttgggggtcc acgatcatct acgaagctca tgttaagggt      480

ctcacttatc tgcacccaga aatacccgtg gaaatacgcg gaacttataa ggcactgggt      540

catcccgtaa tgatcaacta cctgaagcag cttggtatca cggcgcttga actcctccca      600

gttgcacaat ttgcaagtga gccacgactc cagagaatgg ggctttccaa ttactggggt      660

tacaatccag tggccatgtt tgcactccat ccagcgtacg cttgctcccc tgagacggct      720

cttgatgagt ttagagatgc tataaaggca ctgcataagg ctggaataga agtcatcttg      780

gacatagtgc tcaatcacag tgccgagttg gatctggacg ggccgctctt tagcctgcgg      840

gggatagata atcggagtta ctattggatt agggaggacg gcgattacca taattggacg      900

ggttgcggaa atacgctgaa cttgtctcac cctgcggtcg tagactacgc ctccgcatgt      960

cttagatact gggtcgaaac gtgccatgta gatggtttca ggttcgactt ggcggcggtt     1020

atggggagga cgcccgagtt tcggcaagat gcgcccctgt ttacggcgat ccagaactgc     1080

cccgtactgt cacaggttaa actgatcgcg gaaccctggg acattgcccc aggcggttat     1140

caggttggaa acttcccacc actgttcgca gagtggaatg atcactttcg cgatgcagca     1200

cggcggttct ggcttcatta tgatttgccg ctgggcgctt tcgccgggcg gtttgcagca     1260

agttcagatg tatttaagcg caatggtcgc ctcccctccg ctgctattaa cctcgtgaca     1320

gcgcatgatg gattcacgct tagagactgt gtgtgtttca accataaaca taacgaggct     1380

aacggtgagg aaaacagaga tggtacgaat aacaactata gcaataacca cggaaaggaa     1440

ggactcggag gttccttgga tctcgttgaa cgcagacgag attccatcca tgcactgctg     1500

accacgctgt tgttgagcca aggtacacct atgctccttg ctggcgatga gcacgggcat     1560

tcccaacacg gaaacaacaa cgcctattgt caagacaacc agcttacatg gttggactgg     1620

tcacaggcgt ccagtggact gacagcattt actgctgccc tcatacattt gcgcaaacgc     1680

ataccggccc ttgttgagaa ccgctggtgg gaggaaggcg acggtaacgt tcgatggttg     1740

aatcgctacg cgcagcctct cagtacagac gaatggcaga acggaccgaa acagttgcag     1800

attttgctta gcgatcgctt cttgattgcg ataaatgcca cccttgaggt caccgaaatc     1860

gtactgcctg ctggggaatg gcatgccata cctccattcg ccggcgagga taaccctgtg     1920

atcactgctg tctggcaggg cccggcacat ggactttgcg tctttcagag atga           1974


<210>  20
<211>  1974
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon optimized polynucleotide coding sequence (#5) for 
       expressing the bacterial limit dextrin alpha-1,6-glucohydrolase 
       (GlgX)

<400>  20
atgacacaat tggcgattgg aaaaccggca ccccttggtg ctcattatga cggccagggt       60

gtgaatttca ccctgttttc tgcccacgca gaaagggtgg agctttgcgt ctttgacgcg      120

aacgggcagg aacatcggta cgatttgccg ggtcattcag gagatatctg gcatgggtac      180

cttcctgatg ctcgaccagg tcttcggtat gggtatagag ttcatgggcc gtggcagcca      240

gctgaagggc accgctttaa tccagctaaa ctgctcatcg atccgtgtgc gcgccaaata      300

gatggagagt tcaaggataa tcccctcctg cacgcggggc ataatgagcc ggactataga      360

gacaacgctg cgattgctcc gaagtgtgtg gttgtggtcg accactatga ctgggaggat      420

gatgctcccc ctcggacacc atggggctca acgattatct atgaagctca cgtcaaaggg      480

ctgacgtatc tccaccccga gatcccggta gaaattagag ggacctataa agccctcggc      540

catccagtga tgatcaacta tttgaaacaa cttggtataa ctgcgctgga acttctgcct      600

gtagcacagt tcgcgtctga accacgcctt cagagaatgg ggctttccaa ttattgggga      660

tacaatccag tcgcgatgtt tgctcttcac cccgcctatg cctgcagccc tgaaaccgca      720

ctcgacgaat ttagagatgc gataaaagct ctccacaaag cgggcataga ggtaattctt      780

gatatcgtac tcaatcacag cgccgagctt gatcttgatg ggccgctgtt tagtctgcga      840

ggcatagata accgaagtta ctactggatc agagaagacg gagattacca caactggacg      900

ggttgtggga atactctcaa tctgagtcat cccgccgtag tcgattacgc ttccgcatgt      960

cttcgatatt gggttgagac atgccatgtt gatggattca ggttcgacct ggcggcagtc     1020

atgggcagga cccccgagtt tagacaagat gcaccccttt ttacggccat acagaactgc     1080

ccggtcctct cccaggttaa actcatcgcc gagccatggg acattgcacc aggtggctat     1140

caagtcggaa actttccacc actcttcgcc gaatggaatg accatttccg agatgcagct     1200

agaaggtttt ggctccacta cgatctgcct ctcggtgcct ttgcaggacg attcgctgcg     1260

agctctgatg tgtttaagag aaacgggagg cttccgtccg cagctatcaa tcttgtaacg     1320

gcccacgacg gctttactct tcgagattgc gtctgtttca atcacaaaca caatgaggct     1380

aatggagaag aaaaccggga tggaacaaac aataactact caaataacca cggtaaggaa     1440

ggcctcggag ggagccttga cctcgtcgaa cgaagacgcg acagtataca cgctttgctg     1500

accactttgt tgctcagcca aggtacccct atgttgctgg ctggagatga acacggtcac     1560

agccagcacg gaaacaataa tgcgtattgt caagacaacc agcttacatg gcttgactgg     1620

tctcaagcta gttctggact gacagcgttc acggcggctc tgatccattt gagaaagcgc     1680

attccagcgc tggttgagaa ccgctggtgg gaagagggtg atggcaacgt aaggtggctt     1740

aacaggtatg cacaaccgtt gagtaccgat gaatggcaaa atggaccgaa acagctccag     1800

atactcttga gtgaccgctt cttgatcgca ataaacgcta ccctcgaggt taccgagatt     1860

gttttgccag caggagagtg gcacgcaatc ccaccgtttg caggcgaaga taacccagtg     1920

ataacggcag tctggcaggg accagcccac ggcttgtgtg tatttcagcg ctga           1974


<210>  21
<211>  1974
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon optimized polynucleotide coding sequence (#6) for 
       expressing the bacterial limit dextrin alpha-1,6-glucohydrolase 
       (GlgX)

<400>  21
atgacacaac tggcaatcgg taaaccagca ccactcggcg ctcactacga tggccagggt       60

gttaatttca cgttgttttc tgcccatgcg gagcgggtgg aactgtgtgt atttgacgcg      120

aatggacaag agcataggta tgacctcccg ggacattccg gtgacatctg gcatggatac      180

ttgccagatg caaggcccgg actcaggtat ggctaccgag tccacggccc ttggcagcct      240

gcggagggtc atcggtttaa ccctgcaaaa ctcctgatcg acccctgtgc acgacaaatt      300

gacggagaat tcaaagacaa tccgttgctt catgcggggc acaacgagcc ggattacagg      360

gataatgcgg ctattgcccc gaaatgcgtg gttgtcgtcg accactatga ctgggaggac      420

gacgctccac caagaacacc ttgggggtca accataattt atgaagccca cgttaaaggg      480

ctgacctatt tgcaccctga aattccggta gaaatccgag gtacctataa ggccctcggc      540

catccggtaa tgatcaatta tcttaaacag ctgggtatta cagccctgga actgctccca      600

gtagcccagt ttgcgagtga accacggctg caaaggatgg gtctttctaa ctactggggg      660

tataatccag ttgcgatgtt tgctcttcat ccggcttatg catgctcacc tgagactgcc      720

ctggacgagt tccgggatgc aataaaagca ctgcataaag cgggcattga ggttatactt      780

gacattgtat tgaaccactc cgctgaactg gatcttgatg gacctctctt cagcctgcga      840

ggaatagata accgctccta ttactggata agggaagacg gtgactatca caattggacc      900

gggtgcggca acactctcaa tctctcccat cccgccgttg tggattacgc ttcagcgtgc      960

ttgagatatt gggtagagac ttgtcacgta gacggcttcc gattcgatct tgcagctgtc     1020

atgggacgaa ccccagagtt cagacaggat gctcctctgt ttactgcaat tcaaaactgc     1080

ccagtgctgt ctcaggtgaa gttgatagca gagccgtggg acatagcccc cggagggtat     1140

caagttggga actttccgcc cctgttcgcg gagtggaacg atcatttcag ggacgccgca     1200

agacgcttct ggttgcacta cgatctgcct ctcggcgctt ttgcaggtcg gtttgcggct     1260

agtagtgacg tattcaagcg caacggacgg cttccgtctg ccgcaatcaa ccttgtaacc     1320

gcacacgacg gcttcacatt gcgggactgc gtatgtttca atcataagca taacgaggcc     1380

aatggtgagg aaaaccggga tggaaccaac aataactata gtaataatca tggtaaagaa     1440

ggtctgggtg gatcactcga cttggtcgag cgccgacggg attccataca tgcattgctt     1500

actaccctcc tcctctccca aggaacccca atgttgcttg ctggagatga gcacggtcac     1560

agccaacatg ggaataataa tgcttattgt caggataacc aactcacgtg gttggattgg     1620

agtcaagcaa gttcaggcct gaccgctttt acggcagcgt tgatacatct gcgcaaacgc     1680

attcctgcgc tcgttgaaaa cagatggtgg gaagaaggcg acggcaacgt cagatggttg     1740

aatcgatatg cgcaacctct gtcaacggat gaatggcaaa acggtcctaa gcagctccag     1800

atactgctga gtgataggtt cctcattgca atcaacgcga ccctggaagt cacggaaatc     1860

gttttgcccg ctggtgaatg gcacgctata ccgccttttg cgggtgagga taaccccgtt     1920

atcactgccg tgtggcaggg accagcgcac ggcctgtgtg tgttccaacg atga           1974


<210>  22
<211>  1974
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon optimized polynucleotide coding sequence (#7) for 
       expressing the bacterial limit dextrin alpha-1,6-glucohydrolase 
       (GlgX)

<400>  22
atgactcagc ttgccatcgg gaaacccgcc ccgcttggag ctcattacga tggtcaaggc       60

gtaaatttca cactcttctc agcacatgcg gaacgagtcg aactctgtgt atttgatgca      120

aacgggcaag aacatcgcta cgatcttcca ggtcactccg gggacatctg gcatggatac      180

ctccccgacg ctaggccggg acttagatac gggtaccgag tgcatggacc ctggcagcca      240

gcggaggggc atcgatttaa tccagctaag ttgcttatcg atccctgcgc taggcagata      300

gacggcgaat ttaaagataa tccccttttg catgctgggc ataacgagcc tgactatcgc      360

gacaatgctg cgattgcgcc gaagtgcgta gtagtagtcg accactatga ttgggaggac      420

gatgcaccgc cgaggacccc ttgggggagt actatcatat atgaagcaca tgttaaaggt      480

cttacatatc ttcatcccga gatccctgtc gaaatccgag ggacttataa agcattgggg      540

catcctgtta tgatcaatta cttgaaacaa cttggcatca ccgccttgga actgctgccc      600

gtggcccagt tcgcctccga gccccgactg caaaggatgg gactctctaa ttattggggc      660

tataaccccg ttgctatgtt cgcgttgcac ccggcatacg catgttctcc tgagaccgcg      720

ctcgatgaat ttcgagatgc cataaaagct cttcacaagg ccggaattga agtcatactc      780

gacattgttc ttaaccactc agctgaactt gacttggatg ggccgctttt ttctttgcgc      840

gggattgata acagaagtta ctactggata cgggaagacg gtgattatca caattggacg      900

ggttgtggta ataccctcaa tctttcccac ccggctgttg tggattatgc atctgcgtgc      960

cttaggtact gggtcgagac ctgtcatgta gatggctttc gatttgatct cgcggcggtt     1020

atggggagaa cgcccgagtt ccggcaggat gctccacttt tcaccgcaat acagaattgt     1080

cctgtacttt cccaagttaa acttatcgcg gagccatggg acatagctcc tggagggtac     1140

caggttggaa attttccgcc cttgtttgcc gaatggaacg accacttccg cgatgccgct     1200

cggaggttct ggctccatta cgatctccca ctgggagctt tcgcaggtcg ctttgcagcg     1260

tctagcgacg tgtttaagag aaatgggcgg ttgccatccg ctgcgataaa ccttgttacc     1320

gcgcacgacg gctttactct gagagactgt gtttgtttca accataagca caacgaggct     1380

aatggagagg aaaaccgcga cggtacaaat aacaattact ctaataacca tggcaaggag     1440

ggtctgggcg gcagtcttga cctggtcgaa cgaagacggg actctattca tgctctgctc     1500

acaacccttt tgctcagtca ggggacacct atgctgctgg ccggagatga gcacggccac     1560

tcccagcatg gtaataacaa cgcttactgc caggacaacc aacttacgtg gttggattgg     1620

tctcaagcaa gtagtggtct tactgcattt acagcagctc ttatccactt gcgaaaaaga     1680

atacccgctc tggtggagaa ccggtggtgg gaggaaggag acggaaacgt ccggtggctg     1740

aaccgctatg ctcaaccact tagcactgac gagtggcaaa atggcccaaa gcagcttcaa     1800

atactcctca gcgatcgatt cctgattgca ataaatgcca ctcttgaggt caccgagatt     1860

gtccttcccg caggagaatg gcatgccata cccccgtttg ctggagaaga caatccagta     1920

attacggctg tatggcaggg acctgcacac ggcctgtgcg tcttccaaag gtga           1974


<210>  23
<211>  1974
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon optimized polynucleotide coding sequence (#8) for 
       expressing the bacterial limit dextrin alpha-1,6-glucohydrolase 
       (GlgX)

<400>  23
atgactcagc ttgcgattgg gaaacctgct ccgttgggcg ctcattacga tggacaaggc       60

gtcaacttta ctttgtttag cgctcatgca gagagagtag agctgtgcgt gttcgatgcg      120

aatggacaag agcacaggta tgacctcccg ggccactcag gggacatatg gcatggctac      180

ctccccgacg cgagaccggg tttgaggtat ggttaccgcg ttcacggtcc gtggcagccc      240

gcggaaggcc atcggttcaa tcccgccaag ctcctcatcg acccatgtgc ccgacagatt      300

gacggcgagt ttaaagataa tccgctcctc catgcgggtc ataatgaacc ggactaccga      360

gacaatgctg cgatcgcacc taagtgcgtc gttgtagtag accattatga ttgggaagat      420

gacgcaccac cacggactcc ttgggggagc acgataatat acgaggcaca tgtgaaagga      480

ctcacctatc tgcatccgga gattcccgta gaaattcggg gcacttataa ggcgctcggg      540

caccccgtta tgattaatta cctcaagcag ctggggataa ctgcactcga attgcttcca      600

gttgcccaat ttgccagcga gcctcgactt caacgaatgg gcctgagcaa ctattggggt      660

tataatcctg tggcaatgtt tgcgttgcac ccggcatatg cgtgcagccc cgaaacggct      720

ttggatgagt tccgggacgc aataaaggct cttcataagg ccgggataga agtaatcctt      780

gacattgtac ttaatcatag tgcggaactg gacttggatg gccctctgtt cagcttgcgg      840

gggatcgaca acaggagcta ctattggatc cgggaggatg gcgactacca taattggact      900

ggatgtggta acacactgaa tctgagtcat cctgcagtgg ttgactacgc cagtgcatgt      960

ttgagatatt gggttgaaac ctgtcatgtg gacggtttta gattcgatct cgccgcagta     1020

atgggacgaa caccggaatt ccgacaagac gccccgcttt ttacggccat acagaattgt     1080

cccgtgctct ctcaggttaa actgattgct gaaccgtggg atatagcccc cggagggtat     1140

caagtcggca atttccctcc gctgttcgcc gaatggaacg atcattttag agacgccgct     1200

agaagattct ggttgcacta tgacttgccg cttggagctt tcgccggtcg atttgctgcc     1260

tcatcagatg tcttcaagcg caacgggcga ctgccgtccg ctgcaattaa tttggtgacc     1320

gcgcacgatg gattcacact tagggattgc gtatgtttta accataagca caacgaggct     1380

aatggcgaag aaaatagaga cggcacaaat aataactact ccaacaacca cgggaaggaa     1440

ggactcggtg ggagtttgga tctcgttgag cggcgacgcg attctattca tgcgcttctg     1500

acaactcttc ttctctcaca ggggacgcca atgctccttg ccggtgacga gcacggtcat     1560

agccaacatg gcaacaataa tgcatattgt caggacaatc aattgacgtg gcttgactgg     1620

tctcaggcaa gcagtggcct gactgcgttc acagcagccc ttatccatct tcgaaaaagg     1680

atcccagcgc tggtggagaa tcgatggtgg gaagagggtg acgggaatgt gcgctggctg     1740

aatagatatg ctcagccgct gagcacggat gaatggcaga acggaccgaa gcagcttcaa     1800

attctgctgt cagatcgatt tttgatcgcc attaacgcaa cactcgaggt gacagaaatc     1860

gtattgcccg ctggagagtg gcatgccata ccaccatttg ctggtgaaga taatccggtc     1920

atcactgcgg tatggcaagg tccggcacac ggtctgtgtg tttttcaacg atga           1974


<210>  24
<211>  1974
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon optimized polynucleotide coding sequence (#9) for 
       expressing the bacterial limit dextrin alpha-1,6-glucohydrolase 
       (GlgX)

<400>  24
atgacgcagt tggcaatagg taaaccagct ccgcttggag cgcattacga cgggcagggt       60

gtgaatttca cgctgttttc cgcccatgca gaacgcgtag aactgtgcgt gtttgacgca      120

aatggacagg aacatagata cgacctgccg ggtcactctg gcgatatatg gcacggatat      180

ctcccagacg cgaggcccgg tctccggtac ggttaccggg ttcatggtcc gtggcagccc      240

gcagaaggcc atcgctttaa cccagctaag ttgcttatcg acccttgcgc cagacaaata      300

gacggtgaat tcaaggataa tccgctcttg catgcaggac acaacgagcc agattaccgc      360

gacaacgcgg ccattgcccc caagtgcgtt gttgtagttg accattatga ctgggaagac      420

gatgcccctc cacgcacgcc atggggcagc actattatct atgaagccca cgttaaaggt      480

ctcacttatt tgcatcccga gatcccagtt gagatacgag gtacgtataa agctctcggg      540

caccctgtaa tgatcaacta tttgaaacaa ttgggaatta ctgcgctgga gcttttgcca      600

gttgcacagt tcgcgagtga accccgattg cagcgcatgg gtttgagcaa ttattggggt      660

tacaaccctg tagccatgtt cgctctgcat ccagcgtatg cgtgtagtcc cgagaccgct      720

ctcgatgaat ttagggatgc cattaaggca ctccataagg caggcatcga ggtcattctg      780

gatattgtac tcaaccatag cgctgaattg gacctcgatg gcccattgtt ttcattgagg      840

gggatagaca ataggagtta ctattggata cgcgaagacg gcgattatca taactggacg      900

gggtgtggga acacactgaa cctcagccat cctgctgttg tggattatgc ttccgcgtgc      960

ctgaggtatt gggtagagac ctgccacgtt gatggatttc gctttgatct ggcagccgtg     1020

atgggccgca cccctgagtt ccgacaggat gcaccactct ttactgctat ccaaaattgc     1080

ccggttctgt ctcaagtgaa actgattgcg gaaccatggg atatcgcccc aggaggttat     1140

caggtcggca actttcctcc gctcttcgcc gagtggaacg accacttccg agacgctgcc     1200

cgcagattct ggctgcacta cgatctccca cttggtgcgt ttgcggggcg ctttgcagct     1260

tcctccgatg tcttcaagcg gaatgggaga ctgcctagtg cagcgatcaa tctcgtgacg     1320

gcgcacgacg ggttcacact tagagactgc gtctgcttta atcataagca caatgaggcc     1380

aatggggaag agaaccggga cggcacgaac aataactatt caaataacca cggaaaggaa     1440

ggactcgggg gctcactgga tcttgtagaa aggcgacgag attccatcca cgcgctgttg     1500

acaactctgc ttttgagcca gggtacacca atgttgttgg caggggacga acatggacac     1560

agtcagcacg gaaacaataa tgcgtactgc caggacaacc aactcacgtg gcttgattgg     1620

tcacaagcat cctccgggct cacagctttc acagcagcac tgatacacct gcgaaaacga     1680

attccagcac ttgttgaaaa ccgatggtgg gaggagggcg atggaaacgt tagatggctc     1740

aataggtacg ctcagccttt gtccacggac gaatggcaga atggccccaa gcaattgcag     1800

attctcctgt ctgataggtt ccttatagca attaacgcga ccctcgaggt gacagaaatc     1860

gtcctgcccg cgggagaatg gcacgcaatc ccgccgttcg caggtgagga caacccagta     1920

ataacggctg tgtggcaagg accggctcat ggcctttgtg ttttccagag gtga           1974


<210>  25
<211>  1974
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon optimized polynucleotide coding sequence (#10) for 
       expressing the bacterial limit dextrin alpha-1,6-glucohydrolase 
       (GlgX)

<400>  25
atgacacaac ttgctattgg caagccggca ccactcggtg cccattatga cggccagggc       60

gtaaacttca cactcttctc cgcacacgct gaaagagttg aattgtgcgt ctttgacgcc      120

aatggccagg agcaccgata tgatctcccg ggacactccg gtgatatctg gcacggatat      180

ctccctgatg caagaccagg gctgcggtac ggctaccgcg tgcacgggcc ttggcagcca      240

gcagaaggac atcgattcaa tccggccaaa ctgctgattg atccgtgcgc tcggcagatt      300

gacggggagt tcaaagacaa ccccctcctg catgctggcc ataacgaacc cgactatcgc      360

gataatgccg cgatcgcccc gaagtgcgta gtggtagttg accactatga ctgggaagat      420

gatgcaccac cgcgaactcc gtggggcagc acaattatat atgaagcaca tgtcaaaggt      480

ctgacatatt tgcatccgga aatacctgtc gaaatacggg gcacttacaa ggcccttgga      540

caccctgtaa tgattaatta cctgaaacaa ctcggtatca cggcattgga gctgctgccc      600

gtggcccagt ttgcttctga gccacgattg caacggatgg ggttgagtaa ctactggggt      660

tataaccccg ttgccatgtt tgcactccat cccgcgtatg cgtgcagtcc ggagacggcc      720

cttgacgagt tccgagatgc aataaaggcg ctccataagg ccggaataga ggttattctt      780

gatattgtcc ttaaccattc tgctgagttg gatctggacg gtcccctgtt cagtcttaga      840

ggtatagaca atcggtctta ttattggatt cgagaggatg gcgactacca caattggacc      900

ggatgcggaa acacgctcaa cctcagccac ccagccgtag tcgattatgc ctcagcttgc      960

cttcgatatt gggtagagac atgccacgtt gatgggttcc gctttgattt ggcggcagtg     1020

atggggcgga ctccggaatt taggcaggat gctccactgt ttacagcaat tcaaaattgc     1080

cccgtacttt cccaggtgaa actgattgcc gagccctggg acatagcccc cggcgggtac     1140

caagtgggca atttccctcc tttgttcgcc gagtggaacg accacttccg cgacgctgcg     1200

cgcaggtttt ggctccacta tgacttgccc ctcggagcct tcgcaggtcg cttcgcggca     1260

agtagcgatg tcttcaagcg caatggtcgc ttgccctcag cggctattaa tctcgtgacc     1320

gcacacgacg gctttacttt gcgagattgc gtttgcttca atcacaaaca caacgaagcc     1380

aacggcgagg agaataggga tggaacgaat aacaactact caaacaatca cggtaaagag     1440

ggtcttgggg ggagcctgga tcttgttgaa agacggcggg acagtattca cgccctcttg     1500

acgacccttc ttcttagcca gggaactcct atgctcttgg ctggcgatga acacggtcac     1560

tctcaacatg gcaataataa tgcgtattgt caggacaacc aactcacgtg gcttgattgg     1620

agtcaggctt cctctgggct tacggcgttc acggcggccc tgatccacct ccgcaagaga     1680

atccctgctc ttgtagagaa ccgctggtgg gaagaagggg atggaaatgt tcggtggctg     1740

aacagatacg cacaaccact gtctacggac gagtggcaaa atggtccaaa gcagctccag     1800

attctgcttt ctgataggtt cctcattgcc attaatgcca cgcttgaggt gacggagatc     1860

gtcttgccgg cgggtgagtg gcatgcgata ccgcccttcg caggggaaga caatccggtt     1920

attactgcgg tttggcaagg ccccgcacat gggctctgcg tgtttcagag gtga           1974


<210>  26
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  26
gccactggat gcctacaact                                                   20


<210>  27
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  27
cgtgctggtg cagtgtattg                                                   20


<210>  28
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  28
agagggaaat cgtgcgtgac                                                   20


<210>  29
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  29
caatagtgat gacctggccg t                                                 21


<210>  30
<211>  1265
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  LSP-CB dual promoter

<400>  30
gagttaattt ttaaaaagca gtcaaaagtc caagtggccc ttgcgagcat ttactctctc       60

tgtttgctct ggttaataat ctcaggagca caaaattcct tactagtcct agaagttaat      120

ttttaaaaag cagtcaaaag tccaagtcca agtggccctt gcgagcattt actctctctg      180

tttgctctgg ttaataatct caggagcaca aacattcctt actagttcta gagcggccgc      240

cagtgtgctg gaattcggct tttttagggc tggaagctac ctttgacatc atctcctctg      300

cgaatgcatg tataatttct acagaaccta ttagaaagga tcacccagcc tctgcttttg      360

tacaactttc ccttaaaaaa ctgccaatcc cactgctgtt tggcccaata gtgagaactt      420

tttctgctgc ctcttggtgc ttttgcctat ggcccctatt ctgctgctga agacactctt      480

gccagcatgg acttaaaccc ctccagctct gacaatcctc tttctctttt gttttacatg      540

aagggtctgg cagccaaagc aatcactcaa agttcaaacc ttatcatttt ttgctttgtt      600

cctcttggcc ttggttttgt acatcagctt tgaaaatacc atcccagggt taatgctggg      660

gttaatttat aactgagagt gctctagttt tgcaatacag gacatgctat aaaaatggct      720

taaggttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc      780

cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc      840

attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt      900

atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt      960

atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca     1020

tcgctattac catgcatggt cgaggtgagc cccacgttct gcttcactct ccccatctcc     1080

cccccctccc cacccccaat tttgtattta tttatttttt aattattttg tgcagcgagg     1140

ggcggggcgg ggcgaggcgg agaggtgcgg cggcagccaa tcagagcggc gcgctccgaa     1200

agtttccttt tatggcgagg cggcggcggc ggcggcccta taaaaagcga agcgcgcggc     1260

gggcg                                                                 1265


<210>  31
<211>  724
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  LSP promoter

<400>  31
gagttaattt ttaaaaagca gtcaaaagtc caagtggccc ttgcgagcat ttactctctc       60

tgtttgctct ggttaataat ctcaggagca caaaattcct tactagtcct agaagttaat      120

ttttaaaaag cagtcaaaag tccaagtcca agtggccctt gcgagcattt actctctctg      180

tttgctctgg ttaataatct caggagcaca aacattcctt actagttcta gagcggccgc      240

cagtgtgctg gaattcggct tttttagggc tggaagctac ctttgacatc atctcctctg      300

cgaatgcatg tataatttct acagaaccta ttagaaagga tcacccagcc tctgcttttg      360

tacaactttc ccttaaaaaa ctgccaatcc cactgctgtt tggcccaata gtgagaactt      420

tttctgctgc ctcttggtgc ttttgcctat ggcccctatt ctgctgctga agacactctt      480

gccagcatgg acttaaaccc ctccagctct gacaatcctc tttctctttt gttttacatg      540

aagggtctgg cagccaaagc aatcactcaa agttcaaacc ttatcatttt ttgctttgtt      600

cctcttggcc ttggttttgt acatcagctt tgaaaatacc atcccagggt taatgctggg      660

gttaatttat aactgagagt gctctagttt tgcaatacag gacatgctat aaaaatggct      720

taag                                                                   724


<210>  32
<211>  541
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CB promoter

<400>  32
gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg       60

cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg      120

acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca      180

tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc      240

ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc      300

tattaccatg catggtcgag gtgagcccca cgttctgctt cactctcccc atctcccccc      360

cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca gcgaggggcg      420

gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt      480

tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc      540

g                                                                      541


<210>  33
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  33
ccccttaaga gctgcatgtc taagctagac cc                                     32


<210>  34
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  34
cggggtaccc gctggctggc tcctgagt                                          28


<210>  35
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  35
agttctagag cggccgccag                                                   20


<210>  36
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  36
ccccttaagc catttttata gcatgtcctg tattgcaaaa cta                         43


<210>  37
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  37
ccccttaagg ttccgcgtta cataacttac ggtaaat                                37


<210>  38
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  38
gtcgacggta ccgcgcag                                                     18


