                         SEQUENCE LISTING

<110>  Skysong Innovations
       Kong, Wei
       Fu, Lingchen
       Shi, Yixin
 
<120>  TUMOR-DELIVERED MULTI-TARGET THERAPEUTICS FOR COLON CANCER

<130>  112624.01168

<150>  62/809,232
<151>  2019-02-22

<160>  24    

<170>  PatentIn version 3.5

<210>  1
<211>  6192
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- plasmid pK-FZD (pYA3681 encoding human Wnt binding 
       domains of FZD)

<400>  1
agatctagcc cgcctaatga gcgggctttt ttttaattcg caattccccg atgcataatg       60

tgcctgtcaa atggacgaag cagggattct gcaaacccta tgctactccg tcaagccgtc      120

aattgtctga ttcgttacca attatgacaa cttgacggct acatcattca ctttttcttc      180

acaaccggca cggaactcgc tcgggctggc cccggtgcat tttttaaata cccgcgagaa      240

atagagttga tcgtcaaaac caacattgcg accgacggtg gcgataggca tccgggtggt      300

gctcaaaagc agcttcgcct ggctgatacg ttggtcctcg cgccagctta agacgctaat      360

ccctaactgc tggcggaaaa gatgtgacag acgcgacggc gacaagcaaa catgctgtgc      420

gacgctggcg atatcaaaat tgctgtctgc caggtgatcg ctgatgtact gacaagcctc      480

gcgtacccga ttatccatcg gtggatggag cgactcgtta atcgcttcca tgcgccgcag      540

taacaattgc tcaagcagat ttatcgccag cagctccgaa tagcgccctt ccccttgccc      600

ggcgttaatg atttgcccaa acaggtcgct gaaatgcggc tggtgcgctt catccgggcg      660

aaagaacccc gtattggcaa atattgacgg ccagttaagc cattcatgcc agtaggcgcg      720

cggacgaaag taaacccact ggtgatacca ttcgcgagcc tccggatgac gaccgtagtg      780

atgaatctct cctggcggga acagcaaaat atcacccggt cggcaaacaa attctcgtcc      840

ctgatttttc accaccccct gaccgcgaat ggtgagattg agaatataac ctttcattcc      900

cagcggtcgg tcgataaaaa aatcgagata accgttggcc tcaatcggcg ttaaacccgc      960

caccagatgg gcattaaacg agtatcccgg cagcagggga tcattttgcg cttcagccat     1020

acttttcata ctcccgccat tcagagaaga aaccaattgt ccatattgca tcagacattg     1080

ccgtcactgc gtcttttact ggctcttctc gctaaccaaa ccggtaaccc cgcttattaa     1140

aagcattctg taacaaagcg ggaccaaagc catgacaaaa acgcgtaaca aaagtgtcta     1200

taatcacggc agaaaagtcc acattgatta tttgcacggc gtcacacttt gctatgccat     1260

agcattttta tccataagat tagcggatcc tacctgacgc tttttatcgc aactctctac     1320

tgtttctcca tacccgtttt tttgggctag cgaattctga gaacaaacta aatggataaa     1380

tttcgtgttc aggggccaac gaagctccag ggcgaagtca caatttccgg cgctaaaaat     1440

gctgctctgc ctatcctttt tgccgcacta ctggcggaag aaccggtaga gatccagaac     1500

gtcccgaaac tgaaagacgt cgatacatca atgaagctgc taagccagct gggtgcgaaa     1560

gtagaacgta atggttctgt gcatattgat gcccgcgacg ttaatgtatt ctgcgcacct     1620

tacgatctgg ttaaaaccat gcgtgcttct atctgggcgc tggggccgct ggtagcgcgc     1680

tttggtcagg ggcaagtttc actacctggc ggttgtacga tcggtgcgcg tccggttgat     1740

ctacacattt ctggcctcga acaattaggc gcgaccatca aactggaaga aggttacgtt     1800

aaagcttccg tcgatggtcg tttgaaaggt gcacatatcg tgatggataa agtcagcgtt     1860

ggcgcaacgg tgaccatcat gtgtgctgca accctggcgg aaggcaccac gattattgaa     1920

aacgcagcgc gtgaaccgga aatcgtcgat accgcgaact tcctgattac gctgggtgcg     1980

aaaattagcg gtcagggcac cgatcgtatc gtcatcgaag gtgtggaacg tttaggcggc     2040

ggtgtctatc gcgttctgcc ggatcgtatc gaaaccggta ctttcctggt ggcggcggcg     2100

atttctcgcg gcaaaattat ctgccgtaac gcgcagccag atactctcga cgccgtgctg     2160

gcgaaactgc gtgacgctgg agcggacatc gaagtcggcg aagactggat tagcctggat     2220

atgcatggca aacgtccgaa ggctgttaac gtacgtaccg cgccgcatcc ggcattcccg     2280

accgatatgc aggcccagtt cacgctgttg aacctggtgg cagaagggac cgggtttatc     2340

accgaaacgg tctttgaaaa ccgctttatg catgtgccag agctgagccg tatgggcgcg     2400

cacgccgaaa tcgaaagcaa taccgttatt tgtcacggtg ttgaaaaact ttctggcgca     2460

caggttatgg caaccgatct gcgtgcatca gcaagcctgg tgctggctgg ctgtattgcg     2520

gaagggacga cggtggttga tcgtatttat cacatcgatc gtggctacga acgcattgaa     2580

gacaaactgc gcgctttagg tgcaaatatt gagcgtgtga aaggcgaata agaattcagg     2640

aaaaaaacgc tgtgaaaaat gttggtttta tcggctggcg cggaatggtc ggctctgttc     2700

tcatgcaacg catggtagag gagcgcgatt tcgacgctat tcgccctgtt ttcttttcta     2760

cctcccagtt tggacaggcg gcgcccacct tcggcgacac ctccaccggc acgctacagg     2820

acgcttttga tctggatgcg ctaaaagcgc tcgatatcat cgtgacctgc cagggcggcg     2880

attataccaa cgaaatttat ccaaagctgc gcgaaagcgg atggcagggt tactggattg     2940

atgcggcttc tacgctgcgc atgaaagatg atgccattat tattctcgac ccggtcaacc     3000

aggacgtgat taccgacggc ctgaacaatg gcgtgaagac ctttgtgggc ggtaactgta     3060

ccgttagcct gatgttgatg tcgctgggcg gtctctttgc ccataatctc gttgactggg     3120

tatccgtcgc gacctatcag gccgcctccg gcggcggcgc gcgccatatg cgcgagctgt     3180

taacccagat gggtcagttg tatggccatg tcgccgatga actggcgacg ccgtcttccg     3240

caattcttga tattgaacgc aaagttacgg cattgacccg cagcggcgag ctgccggttg     3300

ataactttgg cgtaccgctg gcgggaagcc tgatcccctg gatcgacaaa cagctcgata     3360

acggccagag ccgcgaagag tggaaaggcc aggcggaaac caacaagatt ctcaatactg     3420

cctctgtgat tccggttgat ggtttgtgtg tgcgcgtcgg cgcgctgcgc tgtcacagcc     3480

aggcgttcac catcaagctg aaaaaagagg tatccattcc gacggtggaa gaactgctgg     3540

cggcacataa tccgtgggcg aaagtggtgc cgaacgatcg tgatatcact atgcgcgaat     3600

taaccccggc ggcggtgacc ggcacgttga ctacgccggt tggtcgtctg cgtaagctga     3660

acatggggcc agagttcttg tcggcgttta ccgtaggcga ccagttgtta tggggcgccg     3720

ccgagccgct gcgtcgaatg ctgcgccagt tggcgtagtc tagctgcacg ataccgtcga     3780

cttgtacata gactcgctcc gaaattaaag aacacttaaa ttatctacta aaggaatctt     3840

tagtcaagtt tatttaagat gacttaacta tgaatacaca attgatgggt gagcgtagga     3900

gcatgcttat gcgaaaggcc atcctgacgg atggcctttt tggatcttcc ggaagacctt     3960

ccattctgaa atgagctgtt gacaattaat catccggctc gtataatgtg tggaattgtg     4020

agcggataac aatttcacac aggaaacaga ccatggacca cggcttctgc cagccgatct     4080

ctatcccgct gtgcaccgac atcgcgtaca accagaccat catgccgaac ctgctgggcc     4140

acaccaacca ggaagacgcg ggcctggaag ttcaccagtt ctacccgctg gttaaagttc     4200

agtgctctcc ggaactgcgt ttcttcctgt gctctatgta cgcgccggtt tgcaccgttc     4260

tggaacaggc gatcccgccg tgccgttcta tctgcgaacg tgcgcgtcag ggctgcgaag     4320

cgctgatgaa caaattcggc ttccagtggc cggaacgtct gcgttgcgaa cacttcccgc     4380

gtcacggcgc ggaacagatc tgcgttggcc agaaccactc tgaagacggc gcgccgtaat     4440

agcccgggga tccgtcgacc tgcagccaag ctcccaagct tggctgtttt ggcggatgag     4500

agaagatttt cagcctgata cagattaaat cagaacgcag aagcggtctg ataaaacaga     4560

atttgcctgg cggcagtagc gcggtggtcc cacctgaccc catgccgaac tcagaagtga     4620

aacgccgtag cgccgatggt agtgtggggt ctccccatgc gagagtaggg aactgccagg     4680

catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg     4740

tcggtgaacg ctctcctgag taggacaaat ccgccgggag cggatttgaa cgttgcgaag     4800

caacggcccg gagggtggcg ggcaggacgc ccgccataaa ctgccaggca tcaaattaag     4860

cagaaggcca tcctgacgga tggccttttt gcgtttctac aaactctttt gtttattttt     4920

ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata     4980

atggaagatc ttccaacatc acaggtaaac agaaacgtcg ggtcgatcgg gaaattcttt     5040

cccggacggc gcggggttgg gcaagccgca ggcgcgtcag tgcttttagc gggtgtcggg     5100

gcgcagccat gacccagtca cgtagcgata gcggagtgta tactggctta actatgcggc     5160

atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt     5220

aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc     5280

ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac     5340

agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa     5400

ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca     5460

caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc     5520

gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata     5580

cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta     5640

tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca     5700

gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga     5760

cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg     5820

tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg     5880

tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg     5940

caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag     6000

aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa     6060

cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat     6120

ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc     6180

tgacagtcta ga                                                         6192


<210>  2
<211>  7346
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- plasmid pK-DFZD (pYA4545 encoding human Wnt binding 
       domains of FZD)

<400>  2
gactcttcgc gatgtacggg ccagatatac gcgttaactg cagtctagat tatgcgaaag       60

gccatcctga cggatggcct ttttgtttaa acggatccgc gacattgatt attgactagt      120

tattaatagt aatcaattac ggggtcatta ggggactttc cggggacttt cctccccacg      180

cgggggactt tccgccacgg gcggggactt tccggggact ttccgttcat agcccatata      240

tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc      300

cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc      360

attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt      420

atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt      480

atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca      540

tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg      600

actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc      660

aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg      720

gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca      780

ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc      840

gtttaaactt aagcttggta ccaccatgga ccacggcttc tgccagccca tctccatccc      900

gctgtgcacg gacatcgcct acaaccagac catcatgccc aaccttctgg gccacacgaa      960

ccaggaggac gcaggcctag aggtgcacca gttctatccg ctggtgaagg tgcagtgctc     1020

gcccgaactg cgcttcttcc tgtgctccat gtacgcaccc gtgtgcaccg tgctggaaca     1080

ggccatcccg ccgtgccgct ctatctgtga gcgcgcgcgc cagggctgcg aagccctcat     1140

gaacaagttc ggttttcagt ggcccgagcg cctgcgctgc gagcacttcc cgcgccacgg     1200

cgccgagcag atctgcgtcg gccagaacca ctccgaggac ggagctccct aatagctcga     1260

gaatgcttcg agcagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa     1320

tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca     1380

ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc     1440

agggggagat gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtaaaatcc     1500

gataaggatc gatccggggc atgcaaccag ctgtggaatg tgtgtcagtt agggtgtgga     1560

aagtccccag gctccccagc aggcagaagt atgcaaagca tgtggggatg cggtgggctc     1620

tatggcttct actgggcggt tttatggaca gcaagcgaac cggaattgcc agctggggcg     1680

ccctctggta aggttgggaa gccctgcaaa gtaaactgga tggctttctc gccgccaagg     1740

atctgtcgac ccctagattt cagtgcaatt tatctcttca aatgtagcac ctgaagtcag     1800

ccccatacga tataagttgt tggaagatct agcccgccta atgagcgggc ttttttttaa     1860

ttcgcaattc cccgatgcat aatgtgcctg tcaaatggac gaagcaggga ttctgcaaac     1920

cctatgctac tccgtcaagc cgtcaattgt ctgattcgtt accaattatg acaacttgac     1980

ggctacatca ttcacttttt cttcacaacc ggcacgaaac tcgctcgggc tggccccggt     2040

gcatttttta aatactcgcg agaaatagag ttgatcgtca aaaccaacat tgcgaccgac     2100

ggtggcgata ggcatccggg tagtgctcaa aagcagcttc gcctgactaa tgcgttggtc     2160

ctcgcgccag cttaagacgc taatccctaa ctgctggcgg aaaagatgtg acagacgcga     2220

cggcgacaag caaacatgct gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg     2280

atcgctgatg tactgacaag cctcgcgtac ccgattatcc atcggtggat ggagcgactc     2340

gttaatcgct tccatgcgcc gcagtaacaa ttgctcaagc agatttatcg ccagcagctc     2400

cgaatagcgc ccttcccctt gcccggcgtt aatgatttgc ccaaacaggt cgctgaaatg     2460

cggctggtgc gcttcatccg ggcgaaagaa acccgtattg gcaaatattg acggccagtt     2520

aagccattca tgccagtagg cgcgcggacg aaagtaaacc cactggtgat accattcgcg     2580

agcctccgga tgacgaccgt agtgatgaat ctctcctggc gggaacagca aaatatcacc     2640

cggtcggcag acaaattctc gtccctgatt tttcaccacc ccctgaccgc gaatggtgag     2700

attgagaata taacctttca ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt     2760

ggcctcaatc ggcgttaaac ccgccaccag atgggcgtta aacgagtatc ccggcagcag     2820

gggatcattt tgcgcttcag ccatactttt catactccca ccattcagag aagaaaccaa     2880

ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct tctcgctaac     2940

ccaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca aagccatgac     3000

aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg attatttgca     3060

cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg atcctacctg     3120

acgcttttta tcgcaactct ctactgtttc tccatacccg tttttttggg ctagcgaatt     3180

ctgagaacaa actaagtgga taaatttcgt gttcaggggc caacgaagct ccagggcgaa     3240

gtcacaattt ccggcgctaa aaatgctgct ctgcctatcc tttttgccgc actactggcg     3300

gaagaaccgg tagagatcca gaacgtcccg aaactgaaag acgtcgatac atcaatgaag     3360

ctgctaagcc agctgggtgc gaaagtagaa cgtaatggtt ctgtgcatat tgatgcccgc     3420

gacgttaatg tattctgcgc accttacgat ctggttaaaa ccatgcgtgc ttctatctgg     3480

gcgctggggc cgctggtagc gcgctttggt caggggcaag tttcactacc tggcggttgt     3540

acgatcggtg cgcgtccggt tgatctacac atttctggcc tcgaacaatt aggcgcgacc     3600

atcaaactgg aagaaggtta cgttaaagct tccgtcgatg gtcgtttgaa aggtgcacat     3660

atcgtgatgg ataaagtcag cgttggcgca acggtgacca tcatgtgtgc tgcaaccctg     3720

gcggaaggca ccacgattat tgaaaacgca gcgcgtgaac cggaaatcgt cgataccgcg     3780

aacttcctga ttacgctggg tgcgaaaatt agcggtcagg gcaccgatcg tatcgtcatc     3840

gaaggtgtgg aacgtttagg cggcggtgtc tatcgcgttc tgccggatcg tatcgaaacc     3900

ggtactttcc tggtggcggc ggcgatttct cgcggcaaaa ttatctgccg taacgcgcag     3960

ccagatactc tcgacgccgt gctggcgaaa ctgcgtgacg ctggagcgga catcgaagtc     4020

ggcgaagact ggattagcct ggatatgcat ggcaaacgtc cgaaggctgt taacgtacgt     4080

accgcgccgc atccggcatt cccgaccgat atgcaggccc agttcacgct gttgaacctg     4140

gtggcagaag ggaccgggtt tatcaccgaa acggtctttg aaaaccgctt tatgcatgtg     4200

ccagagctga gccgtatggg cgcgcacgcc gaaatcgaaa gcaataccgt tatttgtcac     4260

ggtgttgaaa aactttctgg cgcacaggtt atggcaaccg atctgcgtgc atcagcaagc     4320

ctggtgctgg ctggctgtat tgcggaaggg acgacggtgg ttgatcgtat ttatcacatc     4380

gatcgtggct acgaacgcat tgaagacaaa ctgcgcgctt taggtgcaaa tattgagcgt     4440

gtgaaaggcg aataagaatt caggaaaaaa acgctgtgaa aaatgttggt tttatcggct     4500

ggcgcggaat ggtcggctct gttctcatgc aacgcatggt agaggagcgc gatttcgacg     4560

ctattcgccc tgttttcttt tctacctccc agtttggaca ggcggcgccc accttcggcg     4620

acacctccac cggcacgcta caggacgctt ttgatctgga tgcgctaaaa gcgctcgata     4680

tcatcgtgac ctgccagggc ggcgattata ccaacgaaat ttatccaaag ctgcgcgaaa     4740

gcggatggca gggttactgg attgatgcgg cttctacgct gcgcatgaaa gatgatgcca     4800

ttattattct cgacccggtc aaccaggacg tgattaccga cggcctgaac aatggcgtga     4860

agacctttgt gggcggtaac tgtaccgtta gcctgatgtt gatgtcgctg ggcggtctct     4920

ttgcccataa tctcgttgac tgggtatccg tcgcgaccta tcaggccgcc tccggcggcg     4980

gcgcgcgcca tatgcgcgag ctgttaaccc agatgggtca gttgtatggc catgtcgccg     5040

atgaactggc gacgccgtct tccgcaattc ttgatattga acgcaaagtt acggcattga     5100

cccgcagcgg cgagctgccg gttgataact ttggcgtacc gctggcggga agcctgatcc     5160

cctggatcga caaacagctc gataacggcc agagccgcga agagtggaaa ggccaggcgg     5220

aaaccaacaa gattctcaat actgcctctg tgattccggt tgatggtttg tgtgtgcgcg     5280

tcggcgcgct gcgctgtcac agccaggcgt tcaccatcaa gctgaaaaaa gaggtatcca     5340

ttccgacggt ggaagaactg ctggcggcac ataatccgtg ggcgaaagtg gtgccgaacg     5400

atcgtgatat cactatgcgc gaattaaccc cggcggcggt gaccggcacg ttgactacgc     5460

cggttggtcg tctgcgtaag ctgaacatgg ggccagagtt cttgtcggcg tttaccgtag     5520

gcgaccagtt gttatggggc gccgccgagc cgctgcgtcg aatgctgcgc cagttggcgt     5580

agtctagctg cacgataccg tcgacttgta catagactcg ctccgaaatt aaagaacact     5640

taaattatct actaaaggaa tctttagtca agtttattta agatgactta actatgaata     5700

cacaattgat gggtgagcgt aggatcttcc attattgaag catttatcag ggttattgtc     5760

tcatgagctt ggctgttttg gcggatgaga gaagattttc agcctgatac agattaaatc     5820

agaacgcaga agcggtctga taaaacagtt tgcctggcgg cagtagcgcg gtggtcccac     5880

ctgaccccat gccgaactca gaagtgaaac gccgtagcgc cgatggtagt gtggggtctc     5940

cccatgcgag agtagggaac tgccaggcat caaataaaac gaaaggctca gtcgaaagac     6000

tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg     6060

ccgggagcgg atttgaacgt tgcgaagcaa cggcccggag ggtggcgggc aggacgcccg     6120

ccataaactg ccaggcatca aattaagcag aaggccatcc tgacggatgg cctttttgcg     6180

tttctacaaa ctctttttgt ttatttttct aaatacattc aaatatgtat ccgctcatga     6240

gacaataacc ctgataaatg cttcaataat ggaagatctt ccaacatcac aggtaaacag     6300

aaacgtcggg tcgatcggga aattctttcc cggacggcgc ggggttgggc aagccgcagg     6360

cgcgtcagtg cttttagcgg gtgtcggggc agccctgaac cagtcacggg atcgatctgt     6420

gcggtatttc acaccgcata caggtggcac ttttcgggga aatgtgcgcg gaacccctat     6480

ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata     6540

aatgcttcaa taatagcacg tgctaaaact tcatttttaa tttaaaagga tctaggtgaa     6600

gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc     6660

gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat     6720

ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga     6780

gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt     6840

ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata     6900

cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac     6960

cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg     7020

ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg     7080

tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag     7140

cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct     7200

ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc     7260

aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctgggctt     7320

ttgctggcct tttgctcaca tgttct                                          7346


<210>  3
<211>  7662
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- plasmid pK-LRP6 (pYA3681 encoding human Wnt binding 
       domains of LRP6)

<400>  3
agatctagcc cgcctaatga gcgggctttt ttttaattcg caattccccg atgcataatg       60

tgcctgtcaa atggacgaag cagggattct gcaaacccta tgctactccg tcaagccgtc      120

aattgtctga ttcgttacca attatgacaa cttgacggct acatcattca ctttttcttc      180

acaaccggca cggaactcgc tcgggctggc cccggtgcat tttttaaata cccgcgagaa      240

atagagttga tcgtcaaaac caacattgcg accgacggtg gcgataggca tccgggtggt      300

gctcaaaagc agcttcgcct ggctgatacg ttggtcctcg cgccagctta agacgctaat      360

ccctaactgc tggcggaaaa gatgtgacag acgcgacggc gacaagcaaa catgctgtgc      420

gacgctggcg atatcaaaat tgctgtctgc caggtgatcg ctgatgtact gacaagcctc      480

gcgtacccga ttatccatcg gtggatggag cgactcgtta atcgcttcca tgcgccgcag      540

taacaattgc tcaagcagat ttatcgccag cagctccgaa tagcgccctt ccccttgccc      600

ggcgttaatg atttgcccaa acaggtcgct gaaatgcggc tggtgcgctt catccgggcg      660

aaagaacccc gtattggcaa atattgacgg ccagttaagc cattcatgcc agtaggcgcg      720

cggacgaaag taaacccact ggtgatacca ttcgcgagcc tccggatgac gaccgtagtg      780

atgaatctct cctggcggga acagcaaaat atcacccggt cggcaaacaa attctcgtcc      840

ctgatttttc accaccccct gaccgcgaat ggtgagattg agaatataac ctttcattcc      900

cagcggtcgg tcgataaaaa aatcgagata accgttggcc tcaatcggcg ttaaacccgc      960

caccagatgg gcattaaacg agtatcccgg cagcagggga tcattttgcg cttcagccat     1020

acttttcata ctcccgccat tcagagaaga aaccaattgt ccatattgca tcagacattg     1080

ccgtcactgc gtcttttact ggctcttctc gctaaccaaa ccggtaaccc cgcttattaa     1140

aagcattctg taacaaagcg ggaccaaagc catgacaaaa acgcgtaaca aaagtgtcta     1200

taatcacggc agaaaagtcc acattgatta tttgcacggc gtcacacttt gctatgccat     1260

agcattttta tccataagat tagcggatcc tacctgacgc tttttatcgc aactctctac     1320

tgtttctcca tacccgtttt tttgggctag cgaattctga gaacaaacta aatggataaa     1380

tttcgtgttc aggggccaac gaagctccag ggcgaagtca caatttccgg cgctaaaaat     1440

gctgctctgc ctatcctttt tgccgcacta ctggcggaag aaccggtaga gatccagaac     1500

gtcccgaaac tgaaagacgt cgatacatca atgaagctgc taagccagct gggtgcgaaa     1560

gtagaacgta atggttctgt gcatattgat gcccgcgacg ttaatgtatt ctgcgcacct     1620

tacgatctgg ttaaaaccat gcgtgcttct atctgggcgc tggggccgct ggtagcgcgc     1680

tttggtcagg ggcaagtttc actacctggc ggttgtacga tcggtgcgcg tccggttgat     1740

ctacacattt ctggcctcga acaattaggc gcgaccatca aactggaaga aggttacgtt     1800

aaagcttccg tcgatggtcg tttgaaaggt gcacatatcg tgatggataa agtcagcgtt     1860

ggcgcaacgg tgaccatcat gtgtgctgca accctggcgg aaggcaccac gattattgaa     1920

aacgcagcgc gtgaaccgga aatcgtcgat accgcgaact tcctgattac gctgggtgcg     1980

aaaattagcg gtcagggcac cgatcgtatc gtcatcgaag gtgtggaacg tttaggcggc     2040

ggtgtctatc gcgttctgcc ggatcgtatc gaaaccggta ctttcctggt ggcggcggcg     2100

atttctcgcg gcaaaattat ctgccgtaac gcgcagccag atactctcga cgccgtgctg     2160

gcgaaactgc gtgacgctgg agcggacatc gaagtcggcg aagactggat tagcctggat     2220

atgcatggca aacgtccgaa ggctgttaac gtacgtaccg cgccgcatcc ggcattcccg     2280

accgatatgc aggcccagtt cacgctgttg aacctggtgg cagaagggac cgggtttatc     2340

accgaaacgg tctttgaaaa ccgctttatg catgtgccag agctgagccg tatgggcgcg     2400

cacgccgaaa tcgaaagcaa taccgttatt tgtcacggtg ttgaaaaact ttctggcgca     2460

caggttatgg caaccgatct gcgtgcatca gcaagcctgg tgctggctgg ctgtattgcg     2520

gaagggacga cggtggttga tcgtatttat cacatcgatc gtggctacga acgcattgaa     2580

gacaaactgc gcgctttagg tgcaaatatt gagcgtgtga aaggcgaata agaattcagg     2640

aaaaaaacgc tgtgaaaaat gttggtttta tcggctggcg cggaatggtc ggctctgttc     2700

tcatgcaacg catggtagag gagcgcgatt tcgacgctat tcgccctgtt ttcttttcta     2760

cctcccagtt tggacaggcg gcgcccacct tcggcgacac ctccaccggc acgctacagg     2820

acgcttttga tctggatgcg ctaaaagcgc tcgatatcat cgtgacctgc cagggcggcg     2880

attataccaa cgaaatttat ccaaagctgc gcgaaagcgg atggcagggt tactggattg     2940

atgcggcttc tacgctgcgc atgaaagatg atgccattat tattctcgac ccggtcaacc     3000

aggacgtgat taccgacggc ctgaacaatg gcgtgaagac ctttgtgggc ggtaactgta     3060

ccgttagcct gatgttgatg tcgctgggcg gtctctttgc ccataatctc gttgactggg     3120

tatccgtcgc gacctatcag gccgcctccg gcggcggcgc gcgccatatg cgcgagctgt     3180

taacccagat gggtcagttg tatggccatg tcgccgatga actggcgacg ccgtcttccg     3240

caattcttga tattgaacgc aaagttacgg cattgacccg cagcggcgag ctgccggttg     3300

ataactttgg cgtaccgctg gcgggaagcc tgatcccctg gatcgacaaa cagctcgata     3360

acggccagag ccgcgaagag tggaaaggcc aggcggaaac caacaagatt ctcaatactg     3420

cctctgtgat tccggttgat ggtttgtgtg tgcgcgtcgg cgcgctgcgc tgtcacagcc     3480

aggcgttcac catcaagctg aaaaaagagg tatccattcc gacggtggaa gaactgctgg     3540

cggcacataa tccgtgggcg aaagtggtgc cgaacgatcg tgatatcact atgcgcgaat     3600

taaccccggc ggcggtgacc ggcacgttga ctacgccggt tggtcgtctg cgtaagctga     3660

acatggggcc agagttcttg tcggcgttta ccgtaggcga ccagttgtta tggggcgccg     3720

ccgagccgct gcgtcgaatg ctgcgccagt tggcgtagtc tagctgcacg ataccgtcga     3780

cttgtacata gactcgctcc gaaattaaag aacacttaaa ttatctacta aaggaatctt     3840

tagtcaagtt tatttaagat gacttaacta tgaatacaca attgatgggt gagcgtagga     3900

gcatgcttat gcgaaaggcc atcctgacgg atggcctttt tggatcttcc ggaagacctt     3960

ccattctgaa atgagctgtt gacaattaat catccggctc gtataatgtg tggaattgtg     4020

agcggataac aatttcacac aggaaacaga ccatgggcat cgtacccgaa gcttttttgt     4080

tgttctcccg tcgcgctgat atccggcgga ttagccttga aacgaacaat aacaatgttg     4140

ctattcccct tacaggcgtc aaagaagcct cggctttgga ttttgatgtt actgataacc     4200

gcatctactg gaccgacatt agcttaaaga caatcagccg ggcgtttatg aacgggtcag     4260

ccttggaaca cgtcgttgaa ttcggcctgg actacccgga gggtatggct gtcgactggc     4320

ttggcaagaa tttatactgg gcagatacag gcaccaatcg catcgaagtt tcaaagttgg     4380

atggacaaca tcgccaagtt ttggtctgga aggacttgga cagccctcgg gccttagcac     4440

tggacccggc cgagggtttt atgtattgga cagagtgggg cgggaaacct aaaatcgatc     4500

gggcagctat ggacggctcg gaacgcacga ctttagttcc gaatgtaggg cgtgccaatg     4560

gattgacgat tgactatgct aagcgccgcc tttattggac agacctggac actaacctta     4620

tcgagtcatc taacatgctt ggcttgaatc gtgaggtaat cgccgatgat ttaccacacc     4680

ccttcggcct gacgcagtac caagattaca tctattggac tgactggtct cggcggagca     4740

ttgaacgggc caataaaacg tcagggcaga atcgtactat cattcaaggg cacttagatt     4800

acgtcatgga tatcttagtt tttcactcca gtcggcaatc aggttggaac gagtgtgcgt     4860

caagtaacgg acactgcagt catttgtgtt tggcagtccc tgtgggcggt ttcgtttgcg     4920

ggtgcccagc ccactactct ttgaacgcag acaatcgcac gtgctcggcg ccaaccacgt     4980

tcctgttgtt tagtcaaaaa tcggctatta atcgtatggt cattgacgag caacaatccc     5040

ccgacatcat ccttccaatc cactcacttc ggaacgtccg tgccatcgat tacgaccctt     5100

tagataaaca gctttactgg atcgattctc ggcagaacat gatccggaaa gcgcaggagg     5160

acgggtcgca aggttttaca gtagtagtat cgtctgtacc ctcacagaat cttgagattc     5220

agccatacga tctgagtatt gatatctatt cgcgttacat ttattggacc tgcgaggcca     5280

ctaatgtaat taacgtgacg cgcctggatg gccgctccgt tggtgtggta ctgaagggag     5340

aacaggatcg tcctcgggct atcgtggtga accccgaaaa aggatatatg tacttcacca     5400

acctgcagga acgtagcccg aaaatcgaac gggcggccct tgatggcaca gaacgggaag     5460

tcttgttttt ctcgggtctt tctaagccaa ttgcgctggc attggacagc cggctgggta     5520

agttattctg ggccgattcg gacctgcgtc gtatcgagag ttctgacctt tcgggagcaa     5580

accgtatcgt attagaggac agtaacattc tgcaaccggt cgggcttaca gtcttcgaaa     5640

attggttgta ttggatcgat aaacagcaac agatgatcga aaaaattgac atgacggggc     5700

gcgagggtcg gacgaaggtc caagcgcgga tcgctcaatt gagcgacatc catgctgtaa     5760

aagaattgaa cttacaggag taccgccagc atccatgtgc acaggataac ggaggatgta     5820

gccacatttg cctggtcaag ggtgatggca caacgcgctg ttcgtgtcct atgcatcttg     5880

tattactgca agacgagttg tcatgctaat agcccgggga tccgtcgacc tgcagccaag     5940

ctcccaagct tggctgtttt ggcggatgag agaagatttt cagcctgata cagattaaat     6000

cagaacgcag aagcggtctg ataaaacaga atttgcctgg cggcagtagc gcggtggtcc     6060

cacctgaccc catgccgaac tcagaagtga aacgccgtag cgccgatggt agtgtggggt     6120

ctccccatgc gagagtaggg aactgccagg catcaaataa aacgaaaggc tcagtcgaaa     6180

gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag taggacaaat     6240

ccgccgggag cggatttgaa cgttgcgaag caacggcccg gagggtggcg ggcaggacgc     6300

ccgccataaa ctgccaggca tcaaattaag cagaaggcca tcctgacgga tggccttttt     6360

gcgtttctac aaactctttt gtttattttt ctaaatacat tcaaatatgt atccgctcat     6420

gagacaataa ccctgataaa tgcttcaata atggaagatc ttccaacatc acaggtaaac     6480

agaaacgtcg ggtcgatcgg gaaattcttt cccggacggc gcggggttgg gcaagccgca     6540

ggcgcgtcag tgcttttagc gggtgtcggg gcgcagccat gacccagtca cgtagcgata     6600

gcggagtgta tactggctta actatgcggc atcagagcag attgtactga gagtgcacca     6660

tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc     6720

cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc     6780

tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat     6840

gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt     6900

ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg     6960

aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc     7020

tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt     7080

ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa     7140

gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta     7200

tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa     7260

caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa     7320

ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt     7380

cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt     7440

ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat     7500

cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat     7560

gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc     7620

aatctaaagt atatatgagt aaacttggtc tgacagtcta ga                        7662


<210>  4
<211>  8816
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- plasmid pK-DLRP6 (pYA4545 encoding human Wnt binding 
       domains of LRP6)

<400>  4
gactcttcgc gatgtacggg ccagatatac gcgttaactg cagtctagat tatgcgaaag       60

gccatcctga cggatggcct ttttgtttaa acggatccgc gacattgatt attgactagt      120

tattaatagt aatcaattac ggggtcatta ggggactttc cggggacttt cctccccacg      180

cgggggactt tccgccacgg gcggggactt tccggggact ttccgttcat agcccatata      240

tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc      300

cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc      360

attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt      420

atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt      480

atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca      540

tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg      600

actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc      660

aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg      720

gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca      780

ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc      840

gtttaaactt aagcttggta ccaccatggg cattgtccca gaggctttcc ttttgttttc      900

acggagagca gatatcagac gaatttctct ggaaacaaac aataataatg tggctattcc      960

actcactggt gtcaaagaag cttctgcttt ggattttgat gtgacagaca accgaattta     1020

ttggactgat atatcactca agaccatcag cagagccttt atgaatggca gtgcactgga     1080

acatgtggta gaattcggct tagattatcc agaaggcatg gcagtagact ggcttgggaa     1140

gaacttgtac tgggcagaca caggaacgaa tcgaattgag gtgtcaaagt tggatgggca     1200

gcaccgacaa gttttggtgt ggaaagacct agatagtccc agagctctcg cgttggaccc     1260

tgccgaagga tttatgtatt ggactgaatg gggtggaaaa cctaagatag acagagctgc     1320

aatggatgga agtgaacgta ctaccttagt tccaaatgtg gggcgggcaa acggcctaac     1380

tattgattat gctaaaagga ggctttattg gacagacctg gacaccaact taatagaatc     1440

ttcaaatatg cttgggctca accgtgaagt tatagcagat gacttgcctc atccttttgg     1500

cttaactcag taccaagatt atatctactg gacggactgg agccgacgca gcattgagcg     1560

tgccaacaaa accagtggcc aaaaccgcac catcattcag ggccatttgg attatgtgat     1620

ggacatcctc gtctttcact catctcgaca gtcagggtgg aatgaatgtg cttccagcaa     1680

tgggcactgc tcccacctct gcttggctgt gccagttggg ggttttgttt gtggatgccc     1740

tgcccactac tctcttaatg ctgacaacag gacttgtagt gctcctacga ctttcctgct     1800

cttcagtcaa aagagtgcca tcaaccgcat ggtgattgat gaacaacaga gccccgacat     1860

catccttccc atccacagcc ttcggaatgt ccgggccatt gactatgacc cactggacaa     1920

gcaactctat tggattgact cacgacaaaa catgatccga aaggcacaag aagatggcag     1980

ccagggcttt actgtggttg tgagctcagt tccgagtcag aacctggaaa tacaacccta     2040

tgacctcagc attgatattt acagccgcta catctactgg acttgtgagg ctaccaatgt     2100

cattaatgtg acaagattag atgggagatc agttggagtg gtgctgaaag gcgagcagga     2160

cagacctcga gccattgtgg taaacccaga gaaagggtat atgtatttta ccaatcttca     2220

ggaaaggtct cctaaaattg aacgggctgc tttggatggg acagaacggg aggtcctctt     2280

tttcagtggc ttaagtaaac caattgcttt agcccttgat agcaggctgg gcaagctctt     2340

ttgggctgat tcagatctcc ggcgaattga aagcagtgat ctctcaggtg ctaaccggat     2400

agtattagaa gactccaata tcttgcagcc tgtgggactt actgtgtttg aaaactggct     2460

ctattggatt gataaacagc agcaaatgat tgaaaaaatt gacatgacag gtcgagaggg     2520

tagaaccaaa gtccaagctc gaattgccca gcttagtgac attcatgcag taaaggagct     2580

gaaccttcaa gaatacagac agcacccttg tgctcaggat aatggtggct gttcacatat     2640

ttgtcttgta aagggggatg gtactacaag gtgttcttgc cccatgcacc tggttctact     2700

tcaagatgag ctatcatgtt aatagctcga gaatgcttcg agcagacatg ataagataca     2760

ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa     2820

tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca     2880

acaattgcat tcattttatg tttcaggttc agggggagat gtgggaggtt ttttaaagca     2940

agtaaaacct ctacaaatgt ggtaaaatcc gataaggatc gatccggggc atgcaaccag     3000

ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt     3060

atgcaaagca tgtggggatg cggtgggctc tatggcttct actgggcggt tttatggaca     3120

gcaagcgaac cggaattgcc agctggggcg ccctctggta aggttgggaa gccctgcaaa     3180

gtaaactgga tggctttctc gccgccaagg atctgtcgac ccctagattt cagtgcaatt     3240

tatctcttca aatgtagcac ctgaagtcag ccccatacga tataagttgt tggaagatct     3300

agcccgccta atgagcgggc ttttttttaa ttcgcaattc cccgatgcat aatgtgcctg     3360

tcaaatggac gaagcaggga ttctgcaaac cctatgctac tccgtcaagc cgtcaattgt     3420

ctgattcgtt accaattatg acaacttgac ggctacatca ttcacttttt cttcacaacc     3480

ggcacgaaac tcgctcgggc tggccccggt gcatttttta aatactcgcg agaaatagag     3540

ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata ggcatccggg tagtgctcaa     3600

aagcagcttc gcctgactaa tgcgttggtc ctcgcgccag cttaagacgc taatccctaa     3660

ctgctggcgg aaaagatgtg acagacgcga cggcgacaag caaacatgct gtgcgacgct     3720

ggcgatatca aaattgctgt ctgccaggtg atcgctgatg tactgacaag cctcgcgtac     3780

ccgattatcc atcggtggat ggagcgactc gttaatcgct tccatgcgcc gcagtaacaa     3840

ttgctcaagc agatttatcg ccagcagctc cgaatagcgc ccttcccctt gcccggcgtt     3900

aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc gcttcatccg ggcgaaagaa     3960

acccgtattg gcaaatattg acggccagtt aagccattca tgccagtagg cgcgcggacg     4020

aaagtaaacc cactggtgat accattcgcg agcctccgga tgacgaccgt agtgatgaat     4080

ctctcctggc gggaacagca aaatatcacc cggtcggcag acaaattctc gtccctgatt     4140

tttcaccacc ccctgaccgc gaatggtgag attgagaata taacctttca ttcccagcgg     4200

tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc ggcgttaaac ccgccaccag     4260

atgggcgtta aacgagtatc ccggcagcag gggatcattt tgcgcttcag ccatactttt     4320

catactccca ccattcagag aagaaaccaa ttgtccatat tgcatcagac attgccgtca     4380

ctgcgtcttt tactggctct tctcgctaac ccaaccggta accccgctta ttaaaagcat     4440

tctgtaacaa agcgggacca aagccatgac aaaaacgcgt aacaaaagtg tctataatca     4500

cggcagaaaa gtccacattg attatttgca cggcgtcaca ctttgctatg ccatagcatt     4560

tttatccata agattagcgg atcctacctg acgcttttta tcgcaactct ctactgtttc     4620

tccatacccg tttttttggg ctagcgaatt ctgagaacaa actaagtgga taaatttcgt     4680

gttcaggggc caacgaagct ccagggcgaa gtcacaattt ccggcgctaa aaatgctgct     4740

ctgcctatcc tttttgccgc actactggcg gaagaaccgg tagagatcca gaacgtcccg     4800

aaactgaaag acgtcgatac atcaatgaag ctgctaagcc agctgggtgc gaaagtagaa     4860

cgtaatggtt ctgtgcatat tgatgcccgc gacgttaatg tattctgcgc accttacgat     4920

ctggttaaaa ccatgcgtgc ttctatctgg gcgctggggc cgctggtagc gcgctttggt     4980

caggggcaag tttcactacc tggcggttgt acgatcggtg cgcgtccggt tgatctacac     5040

atttctggcc tcgaacaatt aggcgcgacc atcaaactgg aagaaggtta cgttaaagct     5100

tccgtcgatg gtcgtttgaa aggtgcacat atcgtgatgg ataaagtcag cgttggcgca     5160

acggtgacca tcatgtgtgc tgcaaccctg gcggaaggca ccacgattat tgaaaacgca     5220

gcgcgtgaac cggaaatcgt cgataccgcg aacttcctga ttacgctggg tgcgaaaatt     5280

agcggtcagg gcaccgatcg tatcgtcatc gaaggtgtgg aacgtttagg cggcggtgtc     5340

tatcgcgttc tgccggatcg tatcgaaacc ggtactttcc tggtggcggc ggcgatttct     5400

cgcggcaaaa ttatctgccg taacgcgcag ccagatactc tcgacgccgt gctggcgaaa     5460

ctgcgtgacg ctggagcgga catcgaagtc ggcgaagact ggattagcct ggatatgcat     5520

ggcaaacgtc cgaaggctgt taacgtacgt accgcgccgc atccggcatt cccgaccgat     5580

atgcaggccc agttcacgct gttgaacctg gtggcagaag ggaccgggtt tatcaccgaa     5640

acggtctttg aaaaccgctt tatgcatgtg ccagagctga gccgtatggg cgcgcacgcc     5700

gaaatcgaaa gcaataccgt tatttgtcac ggtgttgaaa aactttctgg cgcacaggtt     5760

atggcaaccg atctgcgtgc atcagcaagc ctggtgctgg ctggctgtat tgcggaaggg     5820

acgacggtgg ttgatcgtat ttatcacatc gatcgtggct acgaacgcat tgaagacaaa     5880

ctgcgcgctt taggtgcaaa tattgagcgt gtgaaaggcg aataagaatt caggaaaaaa     5940

acgctgtgaa aaatgttggt tttatcggct ggcgcggaat ggtcggctct gttctcatgc     6000

aacgcatggt agaggagcgc gatttcgacg ctattcgccc tgttttcttt tctacctccc     6060

agtttggaca ggcggcgccc accttcggcg acacctccac cggcacgcta caggacgctt     6120

ttgatctgga tgcgctaaaa gcgctcgata tcatcgtgac ctgccagggc ggcgattata     6180

ccaacgaaat ttatccaaag ctgcgcgaaa gcggatggca gggttactgg attgatgcgg     6240

cttctacgct gcgcatgaaa gatgatgcca ttattattct cgacccggtc aaccaggacg     6300

tgattaccga cggcctgaac aatggcgtga agacctttgt gggcggtaac tgtaccgtta     6360

gcctgatgtt gatgtcgctg ggcggtctct ttgcccataa tctcgttgac tgggtatccg     6420

tcgcgaccta tcaggccgcc tccggcggcg gcgcgcgcca tatgcgcgag ctgttaaccc     6480

agatgggtca gttgtatggc catgtcgccg atgaactggc gacgccgtct tccgcaattc     6540

ttgatattga acgcaaagtt acggcattga cccgcagcgg cgagctgccg gttgataact     6600

ttggcgtacc gctggcggga agcctgatcc cctggatcga caaacagctc gataacggcc     6660

agagccgcga agagtggaaa ggccaggcgg aaaccaacaa gattctcaat actgcctctg     6720

tgattccggt tgatggtttg tgtgtgcgcg tcggcgcgct gcgctgtcac agccaggcgt     6780

tcaccatcaa gctgaaaaaa gaggtatcca ttccgacggt ggaagaactg ctggcggcac     6840

ataatccgtg ggcgaaagtg gtgccgaacg atcgtgatat cactatgcgc gaattaaccc     6900

cggcggcggt gaccggcacg ttgactacgc cggttggtcg tctgcgtaag ctgaacatgg     6960

ggccagagtt cttgtcggcg tttaccgtag gcgaccagtt gttatggggc gccgccgagc     7020

cgctgcgtcg aatgctgcgc cagttggcgt agtctagctg cacgataccg tcgacttgta     7080

catagactcg ctccgaaatt aaagaacact taaattatct actaaaggaa tctttagtca     7140

agtttattta agatgactta actatgaata cacaattgat gggtgagcgt aggatcttcc     7200

attattgaag catttatcag ggttattgtc tcatgagctt ggctgttttg gcggatgaga     7260

gaagattttc agcctgatac agattaaatc agaacgcaga agcggtctga taaaacagtt     7320

tgcctggcgg cagtagcgcg gtggtcccac ctgaccccat gccgaactca gaagtgaaac     7380

gccgtagcgc cgatggtagt gtggggtctc cccatgcgag agtagggaac tgccaggcat     7440

caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg     7500

gtgaacgctc tcctgagtag gacaaatccg ccgggagcgg atttgaacgt tgcgaagcaa     7560

cggcccggag ggtggcgggc aggacgcccg ccataaactg ccaggcatca aattaagcag     7620

aaggccatcc tgacggatgg cctttttgcg tttctacaaa ctctttttgt ttatttttct     7680

aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat     7740

ggaagatctt ccaacatcac aggtaaacag aaacgtcggg tcgatcggga aattctttcc     7800

cggacggcgc ggggttgggc aagccgcagg cgcgtcagtg cttttagcgg gtgtcggggc     7860

agccctgaac cagtcacggg atcgatctgt gcggtatttc acaccgcata caggtggcac     7920

ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat     7980

gtatccgctc atgagacaat aaccctgata aatgcttcaa taatagcacg tgctaaaact     8040

tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat     8100

cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc     8160

ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct     8220

accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg     8280

cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca     8340

cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc     8400

tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga     8460

taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac     8520

gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga     8580

agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag     8640

ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg     8700

acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag     8760

caacgcggcc tttttacggt tcctgggctt ttgctggcct tttgctcaca tgttct         8816


<210>  5
<211>  6222
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- plasmid pK-PD-1 (pYA3681 encoding extracellular domain
       of human PD-1)

<400>  5
agatctagcc cgcctaatga gcgggctttt ttttaattcg caattccccg atgcataatg       60

tgcctgtcaa atggacgaag cagggattct gcaaacccta tgctactccg tcaagccgtc      120

aattgtctga ttcgttacca attatgacaa cttgacggct acatcattca ctttttcttc      180

acaaccggca cggaactcgc tcgggctggc cccggtgcat tttttaaata cccgcgagaa      240

atagagttga tcgtcaaaac caacattgcg accgacggtg gcgataggca tccgggtggt      300

gctcaaaagc agcttcgcct ggctgatacg ttggtcctcg cgccagctta agacgctaat      360

ccctaactgc tggcggaaaa gatgtgacag acgcgacggc gacaagcaaa catgctgtgc      420

gacgctggcg atatcaaaat tgctgtctgc caggtgatcg ctgatgtact gacaagcctc      480

gcgtacccga ttatccatcg gtggatggag cgactcgtta atcgcttcca tgcgccgcag      540

taacaattgc tcaagcagat ttatcgccag cagctccgaa tagcgccctt ccccttgccc      600

ggcgttaatg atttgcccaa acaggtcgct gaaatgcggc tggtgcgctt catccgggcg      660

aaagaacccc gtattggcaa atattgacgg ccagttaagc cattcatgcc agtaggcgcg      720

cggacgaaag taaacccact ggtgatacca ttcgcgagcc tccggatgac gaccgtagtg      780

atgaatctct cctggcggga acagcaaaat atcacccggt cggcaaacaa attctcgtcc      840

ctgatttttc accaccccct gaccgcgaat ggtgagattg agaatataac ctttcattcc      900

cagcggtcgg tcgataaaaa aatcgagata accgttggcc tcaatcggcg ttaaacccgc      960

caccagatgg gcattaaacg agtatcccgg cagcagggga tcattttgcg cttcagccat     1020

acttttcata ctcccgccat tcagagaaga aaccaattgt ccatattgca tcagacattg     1080

ccgtcactgc gtcttttact ggctcttctc gctaaccaaa ccggtaaccc cgcttattaa     1140

aagcattctg taacaaagcg ggaccaaagc catgacaaaa acgcgtaaca aaagtgtcta     1200

taatcacggc agaaaagtcc acattgatta tttgcacggc gtcacacttt gctatgccat     1260

agcattttta tccataagat tagcggatcc tacctgacgc tttttatcgc aactctctac     1320

tgtttctcca tacccgtttt tttgggctag cgaattctga gaacaaacta aatggataaa     1380

tttcgtgttc aggggccaac gaagctccag ggcgaagtca caatttccgg cgctaaaaat     1440

gctgctctgc ctatcctttt tgccgcacta ctggcggaag aaccggtaga gatccagaac     1500

gtcccgaaac tgaaagacgt cgatacatca atgaagctgc taagccagct gggtgcgaaa     1560

gtagaacgta atggttctgt gcatattgat gcccgcgacg ttaatgtatt ctgcgcacct     1620

tacgatctgg ttaaaaccat gcgtgcttct atctgggcgc tggggccgct ggtagcgcgc     1680

tttggtcagg ggcaagtttc actacctggc ggttgtacga tcggtgcgcg tccggttgat     1740

ctacacattt ctggcctcga acaattaggc gcgaccatca aactggaaga aggttacgtt     1800

aaagcttccg tcgatggtcg tttgaaaggt gcacatatcg tgatggataa agtcagcgtt     1860

ggcgcaacgg tgaccatcat gtgtgctgca accctggcgg aaggcaccac gattattgaa     1920

aacgcagcgc gtgaaccgga aatcgtcgat accgcgaact tcctgattac gctgggtgcg     1980

aaaattagcg gtcagggcac cgatcgtatc gtcatcgaag gtgtggaacg tttaggcggc     2040

ggtgtctatc gcgttctgcc ggatcgtatc gaaaccggta ctttcctggt ggcggcggcg     2100

atttctcgcg gcaaaattat ctgccgtaac gcgcagccag atactctcga cgccgtgctg     2160

gcgaaactgc gtgacgctgg agcggacatc gaagtcggcg aagactggat tagcctggat     2220

atgcatggca aacgtccgaa ggctgttaac gtacgtaccg cgccgcatcc ggcattcccg     2280

accgatatgc aggcccagtt cacgctgttg aacctggtgg cagaagggac cgggtttatc     2340

accgaaacgg tctttgaaaa ccgctttatg catgtgccag agctgagccg tatgggcgcg     2400

cacgccgaaa tcgaaagcaa taccgttatt tgtcacggtg ttgaaaaact ttctggcgca     2460

caggttatgg caaccgatct gcgtgcatca gcaagcctgg tgctggctgg ctgtattgcg     2520

gaagggacga cggtggttga tcgtatttat cacatcgatc gtggctacga acgcattgaa     2580

gacaaactgc gcgctttagg tgcaaatatt gagcgtgtga aaggcgaata agaattcagg     2640

aaaaaaacgc tgtgaaaaat gttggtttta tcggctggcg cggaatggtc ggctctgttc     2700

tcatgcaacg catggtagag gagcgcgatt tcgacgctat tcgccctgtt ttcttttcta     2760

cctcccagtt tggacaggcg gcgcccacct tcggcgacac ctccaccggc acgctacagg     2820

acgcttttga tctggatgcg ctaaaagcgc tcgatatcat cgtgacctgc cagggcggcg     2880

attataccaa cgaaatttat ccaaagctgc gcgaaagcgg atggcagggt tactggattg     2940

atgcggcttc tacgctgcgc atgaaagatg atgccattat tattctcgac ccggtcaacc     3000

aggacgtgat taccgacggc ctgaacaatg gcgtgaagac ctttgtgggc ggtaactgta     3060

ccgttagcct gatgttgatg tcgctgggcg gtctctttgc ccataatctc gttgactggg     3120

tatccgtcgc gacctatcag gccgcctccg gcggcggcgc gcgccatatg cgcgagctgt     3180

taacccagat gggtcagttg tatggccatg tcgccgatga actggcgacg ccgtcttccg     3240

caattcttga tattgaacgc aaagttacgg cattgacccg cagcggcgag ctgccggttg     3300

ataactttgg cgtaccgctg gcgggaagcc tgatcccctg gatcgacaaa cagctcgata     3360

acggccagag ccgcgaagag tggaaaggcc aggcggaaac caacaagatt ctcaatactg     3420

cctctgtgat tccggttgat ggtttgtgtg tgcgcgtcgg cgcgctgcgc tgtcacagcc     3480

aggcgttcac catcaagctg aaaaaagagg tatccattcc gacggtggaa gaactgctgg     3540

cggcacataa tccgtgggcg aaagtggtgc cgaacgatcg tgatatcact atgcgcgaat     3600

taaccccggc ggcggtgacc ggcacgttga ctacgccggt tggtcgtctg cgtaagctga     3660

acatggggcc agagttcttg tcggcgttta ccgtaggcga ccagttgtta tggggcgccg     3720

ccgagccgct gcgtcgaatg ctgcgccagt tggcgtagtc tagctgcacg ataccgtcga     3780

cttgtacata gactcgctcc gaaattaaag aacacttaaa ttatctacta aaggaatctt     3840

tagtcaagtt tatttaagat gacttaacta tgaatacaca attgatgggt gagcgtagga     3900

gcatgcttat gcgaaaggcc atcctgacgg atggcctttt tggatcttcc ggaagacctt     3960

ccattctgaa atgagctgtt gacaattaat catccggctc gtataatgtg tggaattgtg     4020

agcggataac aatttcacac aggaaacaga ccatgggctt agactcccca gacaggccct     4080

ggaacccccc caccttctcc ccagccctgc tcgtggtgac cgaaggggac aacgccacct     4140

tcacctgcag cttctccaac acatcggaga gcttcgtgct aaactggtac cgcatgagcc     4200

ccagcaacca gacggacaag ctggccgcct tccccgagga ccgcagccag cccggccagg     4260

actgccgctt ccgtgtcaca caactgccca acgggcgtga cttccacatg agcgtggtca     4320

gggcccggcg caatgacagc ggcacctacc tctgtggggc catctccctg gcccccaagg     4380

cgcagatcaa agagagcctg cgggcagagc tcagggtgac agagagaagg gcagaagtgc     4440

ccacagccca ccccagcccc tcaccctaat agcccgggga tccgtcgacc tgcagccaag     4500

ctcccaagct tggctgtttt ggcggatgag agaagatttt cagcctgata cagattaaat     4560

cagaacgcag aagcggtctg ataaaacaga atttgcctgg cggcagtagc gcggtggtcc     4620

cacctgaccc catgccgaac tcagaagtga aacgccgtag cgccgatggt agtgtggggt     4680

ctccccatgc gagagtaggg aactgccagg catcaaataa aacgaaaggc tcagtcgaaa     4740

gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag taggacaaat     4800

ccgccgggag cggatttgaa cgttgcgaag caacggcccg gagggtggcg ggcaggacgc     4860

ccgccataaa ctgccaggca tcaaattaag cagaaggcca tcctgacgga tggccttttt     4920

gcgtttctac aaactctttt gtttattttt ctaaatacat tcaaatatgt atccgctcat     4980

gagacaataa ccctgataaa tgcttcaata atggaagatc ttccaacatc acaggtaaac     5040

agaaacgtcg ggtcgatcgg gaaattcttt cccggacggc gcggggttgg gcaagccgca     5100

ggcgcgtcag tgcttttagc gggtgtcggg gcgcagccat gacccagtca cgtagcgata     5160

gcggagtgta tactggctta actatgcggc atcagagcag attgtactga gagtgcacca     5220

tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc     5280

cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc     5340

tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat     5400

gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt     5460

ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg     5520

aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc     5580

tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt     5640

ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa     5700

gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta     5760

tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa     5820

caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa     5880

ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt     5940

cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt     6000

ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat     6060

cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat     6120

gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc     6180

aatctaaagt atatatgagt aaacttggtc tgacagtcta ga                        6222


<210>  6
<211>  7376
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- plasmid pK-DPD-1 (pYA4545 encoding extracellular 
       domain of human PD-1)

<400>  6
gactcttcgc gatgtacggg ccagatatac gcgttaactg cagtctagat tatgcgaaag       60

gccatcctga cggatggcct ttttgtttaa acggatccgc gacattgatt attgactagt      120

tattaatagt aatcaattac ggggtcatta ggggactttc cggggacttt cctccccacg      180

cgggggactt tccgccacgg gcggggactt tccggggact ttccgttcat agcccatata      240

tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc      300

cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc      360

attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt      420

atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt      480

atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca      540

tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg      600

actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc      660

aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg      720

gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca      780

ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc      840

gtttaaactt aagcttggta ccaccatggg cttagactcc ccagacaggc cctggaaccc      900

ccccaccttc tccccagccc tgctcgtggt gaccgaaggg gacaacgcca ccttcacctg      960

cagcttctcc aacacatcgg agagcttcgt gctaaactgg taccgcatga gccccagcaa     1020

ccagacggac aagctggccg ccttccccga ggaccgcagc cagcccggcc aggactgccg     1080

cttccgtgtc acacaactgc ccaacgggcg tgacttccac atgagcgtgg tcagggcccg     1140

gcgcaatgac agcggcacct acctctgtgg ggccatctcc ctggccccca aggcgcagat     1200

caaagagagc ctgcgggcag agctcagggt gacagagaga agggcagaag tgcccacagc     1260

ccaccccagc ccctcaccct aatagctcga gaatgcttcg agcagacatg ataagataca     1320

ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa     1380

tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca     1440

acaattgcat tcattttatg tttcaggttc agggggagat gtgggaggtt ttttaaagca     1500

agtaaaacct ctacaaatgt ggtaaaatcc gataaggatc gatccggggc atgcaaccag     1560

ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt     1620

atgcaaagca tgtggggatg cggtgggctc tatggcttct actgggcggt tttatggaca     1680

gcaagcgaac cggaattgcc agctggggcg ccctctggta aggttgggaa gccctgcaaa     1740

gtaaactgga tggctttctc gccgccaagg atctgtcgac ccctagattt cagtgcaatt     1800

tatctcttca aatgtagcac ctgaagtcag ccccatacga tataagttgt tggaagatct     1860

agcccgccta atgagcgggc ttttttttaa ttcgcaattc cccgatgcat aatgtgcctg     1920

tcaaatggac gaagcaggga ttctgcaaac cctatgctac tccgtcaagc cgtcaattgt     1980

ctgattcgtt accaattatg acaacttgac ggctacatca ttcacttttt cttcacaacc     2040

ggcacgaaac tcgctcgggc tggccccggt gcatttttta aatactcgcg agaaatagag     2100

ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata ggcatccggg tagtgctcaa     2160

aagcagcttc gcctgactaa tgcgttggtc ctcgcgccag cttaagacgc taatccctaa     2220

ctgctggcgg aaaagatgtg acagacgcga cggcgacaag caaacatgct gtgcgacgct     2280

ggcgatatca aaattgctgt ctgccaggtg atcgctgatg tactgacaag cctcgcgtac     2340

ccgattatcc atcggtggat ggagcgactc gttaatcgct tccatgcgcc gcagtaacaa     2400

ttgctcaagc agatttatcg ccagcagctc cgaatagcgc ccttcccctt gcccggcgtt     2460

aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc gcttcatccg ggcgaaagaa     2520

acccgtattg gcaaatattg acggccagtt aagccattca tgccagtagg cgcgcggacg     2580

aaagtaaacc cactggtgat accattcgcg agcctccgga tgacgaccgt agtgatgaat     2640

ctctcctggc gggaacagca aaatatcacc cggtcggcag acaaattctc gtccctgatt     2700

tttcaccacc ccctgaccgc gaatggtgag attgagaata taacctttca ttcccagcgg     2760

tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc ggcgttaaac ccgccaccag     2820

atgggcgtta aacgagtatc ccggcagcag gggatcattt tgcgcttcag ccatactttt     2880

catactccca ccattcagag aagaaaccaa ttgtccatat tgcatcagac attgccgtca     2940

ctgcgtcttt tactggctct tctcgctaac ccaaccggta accccgctta ttaaaagcat     3000

tctgtaacaa agcgggacca aagccatgac aaaaacgcgt aacaaaagtg tctataatca     3060

cggcagaaaa gtccacattg attatttgca cggcgtcaca ctttgctatg ccatagcatt     3120

tttatccata agattagcgg atcctacctg acgcttttta tcgcaactct ctactgtttc     3180

tccatacccg tttttttggg ctagcgaatt ctgagaacaa actaagtgga taaatttcgt     3240

gttcaggggc caacgaagct ccagggcgaa gtcacaattt ccggcgctaa aaatgctgct     3300

ctgcctatcc tttttgccgc actactggcg gaagaaccgg tagagatcca gaacgtcccg     3360

aaactgaaag acgtcgatac atcaatgaag ctgctaagcc agctgggtgc gaaagtagaa     3420

cgtaatggtt ctgtgcatat tgatgcccgc gacgttaatg tattctgcgc accttacgat     3480

ctggttaaaa ccatgcgtgc ttctatctgg gcgctggggc cgctggtagc gcgctttggt     3540

caggggcaag tttcactacc tggcggttgt acgatcggtg cgcgtccggt tgatctacac     3600

atttctggcc tcgaacaatt aggcgcgacc atcaaactgg aagaaggtta cgttaaagct     3660

tccgtcgatg gtcgtttgaa aggtgcacat atcgtgatgg ataaagtcag cgttggcgca     3720

acggtgacca tcatgtgtgc tgcaaccctg gcggaaggca ccacgattat tgaaaacgca     3780

gcgcgtgaac cggaaatcgt cgataccgcg aacttcctga ttacgctggg tgcgaaaatt     3840

agcggtcagg gcaccgatcg tatcgtcatc gaaggtgtgg aacgtttagg cggcggtgtc     3900

tatcgcgttc tgccggatcg tatcgaaacc ggtactttcc tggtggcggc ggcgatttct     3960

cgcggcaaaa ttatctgccg taacgcgcag ccagatactc tcgacgccgt gctggcgaaa     4020

ctgcgtgacg ctggagcgga catcgaagtc ggcgaagact ggattagcct ggatatgcat     4080

ggcaaacgtc cgaaggctgt taacgtacgt accgcgccgc atccggcatt cccgaccgat     4140

atgcaggccc agttcacgct gttgaacctg gtggcagaag ggaccgggtt tatcaccgaa     4200

acggtctttg aaaaccgctt tatgcatgtg ccagagctga gccgtatggg cgcgcacgcc     4260

gaaatcgaaa gcaataccgt tatttgtcac ggtgttgaaa aactttctgg cgcacaggtt     4320

atggcaaccg atctgcgtgc atcagcaagc ctggtgctgg ctggctgtat tgcggaaggg     4380

acgacggtgg ttgatcgtat ttatcacatc gatcgtggct acgaacgcat tgaagacaaa     4440

ctgcgcgctt taggtgcaaa tattgagcgt gtgaaaggcg aataagaatt caggaaaaaa     4500

acgctgtgaa aaatgttggt tttatcggct ggcgcggaat ggtcggctct gttctcatgc     4560

aacgcatggt agaggagcgc gatttcgacg ctattcgccc tgttttcttt tctacctccc     4620

agtttggaca ggcggcgccc accttcggcg acacctccac cggcacgcta caggacgctt     4680

ttgatctgga tgcgctaaaa gcgctcgata tcatcgtgac ctgccagggc ggcgattata     4740

ccaacgaaat ttatccaaag ctgcgcgaaa gcggatggca gggttactgg attgatgcgg     4800

cttctacgct gcgcatgaaa gatgatgcca ttattattct cgacccggtc aaccaggacg     4860

tgattaccga cggcctgaac aatggcgtga agacctttgt gggcggtaac tgtaccgtta     4920

gcctgatgtt gatgtcgctg ggcggtctct ttgcccataa tctcgttgac tgggtatccg     4980

tcgcgaccta tcaggccgcc tccggcggcg gcgcgcgcca tatgcgcgag ctgttaaccc     5040

agatgggtca gttgtatggc catgtcgccg atgaactggc gacgccgtct tccgcaattc     5100

ttgatattga acgcaaagtt acggcattga cccgcagcgg cgagctgccg gttgataact     5160

ttggcgtacc gctggcggga agcctgatcc cctggatcga caaacagctc gataacggcc     5220

agagccgcga agagtggaaa ggccaggcgg aaaccaacaa gattctcaat actgcctctg     5280

tgattccggt tgatggtttg tgtgtgcgcg tcggcgcgct gcgctgtcac agccaggcgt     5340

tcaccatcaa gctgaaaaaa gaggtatcca ttccgacggt ggaagaactg ctggcggcac     5400

ataatccgtg ggcgaaagtg gtgccgaacg atcgtgatat cactatgcgc gaattaaccc     5460

cggcggcggt gaccggcacg ttgactacgc cggttggtcg tctgcgtaag ctgaacatgg     5520

ggccagagtt cttgtcggcg tttaccgtag gcgaccagtt gttatggggc gccgccgagc     5580

cgctgcgtcg aatgctgcgc cagttggcgt agtctagctg cacgataccg tcgacttgta     5640

catagactcg ctccgaaatt aaagaacact taaattatct actaaaggaa tctttagtca     5700

agtttattta agatgactta actatgaata cacaattgat gggtgagcgt aggatcttcc     5760

attattgaag catttatcag ggttattgtc tcatgagctt ggctgttttg gcggatgaga     5820

gaagattttc agcctgatac agattaaatc agaacgcaga agcggtctga taaaacagtt     5880

tgcctggcgg cagtagcgcg gtggtcccac ctgaccccat gccgaactca gaagtgaaac     5940

gccgtagcgc cgatggtagt gtggggtctc cccatgcgag agtagggaac tgccaggcat     6000

caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg     6060

gtgaacgctc tcctgagtag gacaaatccg ccgggagcgg atttgaacgt tgcgaagcaa     6120

cggcccggag ggtggcgggc aggacgcccg ccataaactg ccaggcatca aattaagcag     6180

aaggccatcc tgacggatgg cctttttgcg tttctacaaa ctctttttgt ttatttttct     6240

aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat     6300

ggaagatctt ccaacatcac aggtaaacag aaacgtcggg tcgatcggga aattctttcc     6360

cggacggcgc ggggttgggc aagccgcagg cgcgtcagtg cttttagcgg gtgtcggggc     6420

agccctgaac cagtcacggg atcgatctgt gcggtatttc acaccgcata caggtggcac     6480

ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat     6540

gtatccgctc atgagacaat aaccctgata aatgcttcaa taatagcacg tgctaaaact     6600

tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat     6660

cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc     6720

ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct     6780

accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg     6840

cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca     6900

cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc     6960

tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga     7020

taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac     7080

gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga     7140

agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag     7200

ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg     7260

acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag     7320

caacgcggcc tttttacggt tcctgggctt ttgctggcct tttgctcaca tgttct         7376


<210>  7
<211>  6840
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- plasmid pK-SIRP-alpha (pYA3681 encoding extracellular 
       domain of human SIRP-alpha)

<400>  7
agatctagcc cgcctaatga gcgggctttt ttttaattcg caattccccg atgcataatg       60

tgcctgtcaa atggacgaag cagggattct gcaaacccta tgctactccg tcaagccgtc      120

aattgtctga ttcgttacca attatgacaa cttgacggct acatcattca ctttttcttc      180

acaaccggca cggaactcgc tcgggctggc cccggtgcat tttttaaata cccgcgagaa      240

atagagttga tcgtcaaaac caacattgcg accgacggtg gcgataggca tccgggtggt      300

gctcaaaagc agcttcgcct ggctgatacg ttggtcctcg cgccagctta agacgctaat      360

ccctaactgc tggcggaaaa gatgtgacag acgcgacggc gacaagcaaa catgctgtgc      420

gacgctggcg atatcaaaat tgctgtctgc caggtgatcg ctgatgtact gacaagcctc      480

gcgtacccga ttatccatcg gtggatggag cgactcgtta atcgcttcca tgcgccgcag      540

taacaattgc tcaagcagat ttatcgccag cagctccgaa tagcgccctt ccccttgccc      600

ggcgttaatg atttgcccaa acaggtcgct gaaatgcggc tggtgcgctt catccgggcg      660

aaagaacccc gtattggcaa atattgacgg ccagttaagc cattcatgcc agtaggcgcg      720

cggacgaaag taaacccact ggtgatacca ttcgcgagcc tccggatgac gaccgtagtg      780

atgaatctct cctggcggga acagcaaaat atcacccggt cggcaaacaa attctcgtcc      840

ctgatttttc accaccccct gaccgcgaat ggtgagattg agaatataac ctttcattcc      900

cagcggtcgg tcgataaaaa aatcgagata accgttggcc tcaatcggcg ttaaacccgc      960

caccagatgg gcattaaacg agtatcccgg cagcagggga tcattttgcg cttcagccat     1020

acttttcata ctcccgccat tcagagaaga aaccaattgt ccatattgca tcagacattg     1080

ccgtcactgc gtcttttact ggctcttctc gctaaccaaa ccggtaaccc cgcttattaa     1140

aagcattctg taacaaagcg ggaccaaagc catgacaaaa acgcgtaaca aaagtgtcta     1200

taatcacggc agaaaagtcc acattgatta tttgcacggc gtcacacttt gctatgccat     1260

agcattttta tccataagat tagcggatcc tacctgacgc tttttatcgc aactctctac     1320

tgtttctcca tacccgtttt tttgggctag cgaattctga gaacaaacta aatggataaa     1380

tttcgtgttc aggggccaac gaagctccag ggcgaagtca caatttccgg cgctaaaaat     1440

gctgctctgc ctatcctttt tgccgcacta ctggcggaag aaccggtaga gatccagaac     1500

gtcccgaaac tgaaagacgt cgatacatca atgaagctgc taagccagct gggtgcgaaa     1560

gtagaacgta atggttctgt gcatattgat gcccgcgacg ttaatgtatt ctgcgcacct     1620

tacgatctgg ttaaaaccat gcgtgcttct atctgggcgc tggggccgct ggtagcgcgc     1680

tttggtcagg ggcaagtttc actacctggc ggttgtacga tcggtgcgcg tccggttgat     1740

ctacacattt ctggcctcga acaattaggc gcgaccatca aactggaaga aggttacgtt     1800

aaagcttccg tcgatggtcg tttgaaaggt gcacatatcg tgatggataa agtcagcgtt     1860

ggcgcaacgg tgaccatcat gtgtgctgca accctggcgg aaggcaccac gattattgaa     1920

aacgcagcgc gtgaaccgga aatcgtcgat accgcgaact tcctgattac gctgggtgcg     1980

aaaattagcg gtcagggcac cgatcgtatc gtcatcgaag gtgtggaacg tttaggcggc     2040

ggtgtctatc gcgttctgcc ggatcgtatc gaaaccggta ctttcctggt ggcggcggcg     2100

atttctcgcg gcaaaattat ctgccgtaac gcgcagccag atactctcga cgccgtgctg     2160

gcgaaactgc gtgacgctgg agcggacatc gaagtcggcg aagactggat tagcctggat     2220

atgcatggca aacgtccgaa ggctgttaac gtacgtaccg cgccgcatcc ggcattcccg     2280

accgatatgc aggcccagtt cacgctgttg aacctggtgg cagaagggac cgggtttatc     2340

accgaaacgg tctttgaaaa ccgctttatg catgtgccag agctgagccg tatgggcgcg     2400

cacgccgaaa tcgaaagcaa taccgttatt tgtcacggtg ttgaaaaact ttctggcgca     2460

caggttatgg caaccgatct gcgtgcatca gcaagcctgg tgctggctgg ctgtattgcg     2520

gaagggacga cggtggttga tcgtatttat cacatcgatc gtggctacga acgcattgaa     2580

gacaaactgc gcgctttagg tgcaaatatt gagcgtgtga aaggcgaata agaattcagg     2640

aaaaaaacgc tgtgaaaaat gttggtttta tcggctggcg cggaatggtc ggctctgttc     2700

tcatgcaacg catggtagag gagcgcgatt tcgacgctat tcgccctgtt ttcttttcta     2760

cctcccagtt tggacaggcg gcgcccacct tcggcgacac ctccaccggc acgctacagg     2820

acgcttttga tctggatgcg ctaaaagcgc tcgatatcat cgtgacctgc cagggcggcg     2880

attataccaa cgaaatttat ccaaagctgc gcgaaagcgg atggcagggt tactggattg     2940

atgcggcttc tacgctgcgc atgaaagatg atgccattat tattctcgac ccggtcaacc     3000

aggacgtgat taccgacggc ctgaacaatg gcgtgaagac ctttgtgggc ggtaactgta     3060

ccgttagcct gatgttgatg tcgctgggcg gtctctttgc ccataatctc gttgactggg     3120

tatccgtcgc gacctatcag gccgcctccg gcggcggcgc gcgccatatg cgcgagctgt     3180

taacccagat gggtcagttg tatggccatg tcgccgatga actggcgacg ccgtcttccg     3240

caattcttga tattgaacgc aaagttacgg cattgacccg cagcggcgag ctgccggttg     3300

ataactttgg cgtaccgctg gcgggaagcc tgatcccctg gatcgacaaa cagctcgata     3360

acggccagag ccgcgaagag tggaaaggcc aggcggaaac caacaagatt ctcaatactg     3420

cctctgtgat tccggttgat ggtttgtgtg tgcgcgtcgg cgcgctgcgc tgtcacagcc     3480

aggcgttcac catcaagctg aaaaaagagg tatccattcc gacggtggaa gaactgctgg     3540

cggcacataa tccgtgggcg aaagtggtgc cgaacgatcg tgatatcact atgcgcgaat     3600

taaccccggc ggcggtgacc ggcacgttga ctacgccggt tggtcgtctg cgtaagctga     3660

acatggggcc agagttcttg tcggcgttta ccgtaggcga ccagttgtta tggggcgccg     3720

ccgagccgct gcgtcgaatg ctgcgccagt tggcgtagtc tagctgcacg ataccgtcga     3780

cttgtacata gactcgctcc gaaattaaag aacacttaaa ttatctacta aaggaatctt     3840

tagtcaagtt tatttaagat gacttaacta tgaatacaca attgatgggt gagcgtagga     3900

gcatgcttat gcgaaaggcc atcctgacgg atggcctttt tggatcttcc ggaagacctt     3960

ccattctgaa atgagctgtt gacaattaat catccggctc gtataatgtg tggaattgtg     4020

agcggataac aatttcacac aggaaacaga ccatgggcgt tgcgggcgaa gaagaactgc     4080

aggttatcca gccggacaaa tctgtttctg ttgcggcggg cgaatctgcg atcctgcact     4140

gcaccgttac ctctctgatc ccggttggcc cgatccagtg gttccgtggc gcgggcccgg     4200

cgcgtgaact gatctacaac cagaaagaag gccacttccc gcgtgttacc accgtttctg     4260

aatctaccaa acgtgaaaac atggacttct ctatctctat ctctaacatc accccggcgg     4320

acgcgggcac ctactactgc gttaaattcc gtaaaggctc tccggacacc gaattcaaat     4380

ctggcgcggg caccgaactg tctgttcgtg cgaaaccgtc tgcgccagtt gtaagcggcc     4440

cggcggcgcg tgcgaccccg cagcatactg ttagcttcac ctgcgaatcg cacggcttct     4500

ctccgcgtga catcaccctg aaatggttca aaaacggcaa cgaactgtct gacttccaga     4560

ccaacgttga cccggttggc gaatctgttt cttactctat ccactctacc gcgaaagttg     4620

ttctgacccg tgaagacgtt cactctcagg ttatctgcga agttgcgcac gttaccctgc     4680

agggcgaccc gctgcgtggc accgcgaacc tgtctgaaac catccgtgtt ccgccgaccc     4740

tggaagttac ccagcagccg gttcgtgcgg aaaaccaggt taacgttacc tgccaggttc     4800

gtaaattcta cccgcagcgt ctgcagctga cctggctgga gaatggcaac gtaagccgta     4860

ccgaaaccgc gtctaccgtt accgaaaaca aagacggcac ctacaactgg atgtcttggc     4920

tgctggttaa cgtttctgcg caccgtgacg acgttaaact gacctgccag gttgaacatg     4980

atggccagcc ggcggtaagc aaatctcacg acctgaaagt ttctgcgcac ccgaaagaac     5040

agggctctaa caccgcggcg gaaaacaccg gctctaacga acgttaatag cccggggatc     5100

cgtcgacctg cagccaagct cccaagcttg gctgttttgg cggatgagag aagattttca     5160

gcctgataca gattaaatca gaacgcagaa gcggtctgat aaaacagaat ttgcctggcg     5220

gcagtagcgc ggtggtccca cctgacccca tgccgaactc agaagtgaaa cgccgtagcg     5280

ccgatggtag tgtggggtct ccccatgcga gagtagggaa ctgccaggca tcaaataaaa     5340

cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct     5400

ctcctgagta ggacaaatcc gccgggagcg gatttgaacg ttgcgaagca acggcccgga     5460

gggtggcggg caggacgccc gccataaact gccaggcatc aaattaagca gaaggccatc     5520

ctgacggatg gcctttttgc gtttctacaa actcttttgt ttatttttct aaatacattc     5580

aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat ggaagatctt     5640

ccaacatcac aggtaaacag aaacgtcggg tcgatcggga aattctttcc cggacggcgc     5700

ggggttgggc aagccgcagg cgcgtcagtg cttttagcgg gtgtcggggc gcagccatga     5760

cccagtcacg tagcgatagc ggagtgtata ctggcttaac tatgcggcat cagagcagat     5820

tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata     5880

ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct     5940

gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga     6000

taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc     6060

cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg     6120

ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg     6180

aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt     6240

tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt     6300

gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg     6360

cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact     6420

ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt     6480

cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct     6540

gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac     6600

cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc     6660

tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg     6720

ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta     6780

aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagtctaga     6840


<210>  8
<211>  7994
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- plasmid pK-DSIRP-alpha (pYA4545 encoding extracellular
       domain of human SIRP-alpha)

<400>  8
gactcttcgc gatgtacggg ccagatatac gcgttaactg cagtctagat tatgcgaaag       60

gccatcctga cggatggcct ttttgtttaa acggatccgc gacattgatt attgactagt      120

tattaatagt aatcaattac ggggtcatta ggggactttc cggggacttt cctccccacg      180

cgggggactt tccgccacgg gcggggactt tccggggact ttccgttcat agcccatata      240

tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc      300

cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc      360

attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt      420

atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt      480

atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca      540

tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg      600

actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc      660

aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg      720

gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca      780

ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc      840

gtttaaactt aagcttggta ccaccatggg agtggcgggt gaggaggagc tgcaggtgat      900

tcagcctgac aagtccgtat cagttgcagc tggagagtcg gccattctgc actgcactgt      960

gacctccctg atccctgtgg ggcccatcca gtggttcaga ggagctggac cagcccggga     1020

attaatctac aatcaaaaag aaggccactt cccccgggta acaactgttt cagagtccac     1080

aaagagagaa aacatggact tttccatcag catcagtaac atcaccccag cagatgccgg     1140

cacctactac tgtgtgaagt tccggaaagg gagccctgac acggagttta agtctggagc     1200

aggcactgag ctgtctgtgc gtgccaaacc ctctgccccc gtggtatcgg gccctgcggc     1260

gagggccaca cctcagcaca cagtgagctt cacctgcgag tcccacggct tctcacccag     1320

agacatcacc ctgaaatggt tcaaaaatgg gaatgagctc tcagacttcc agaccaacgt     1380

ggaccccgta ggagagagcg tgtcctacag catccacagc acagccaagg tggtgctgac     1440

ccgcgaggac gttcactctc aagtcatctg cgaggtggcc cacgtcacct tgcaggggga     1500

ccctcttcgt gggactgcca acttgtctga gaccatccga gttccaccca ccttggaggt     1560

tactcaacag cccgtgaggg cagagaacca ggtgaatgtc acctgccagg tgaggaagtt     1620

ctacccccag agactacagc tgacctggtt ggagaatgga aacgtgtccc ggacagaaac     1680

ggcctcaacc gttacagaga acaaggatgg tacctacaac tggatgagct ggctcctggt     1740

gaatgtatct gcccacaggg atgatgtgaa gctcacctgc caggtggagc atgacgggca     1800

gccagcggtc agcaaaagcc atgacctgaa ggtctcagcc cacccgaagg agcagggctc     1860

aaataccgcc gctgagaaca ctggatctaa tgaacggtaa tagctcgaga atgcttcgag     1920

cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa     1980

aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca     2040

ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag ggggagatgt     2100

gggaggtttt ttaaagcaag taaaacctct acaaatgtgg taaaatccga taaggatcga     2160

tccggggcat gcaaccagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc     2220

tccccagcag gcagaagtat gcaaagcatg tggggatgcg gtgggctcta tggcttctac     2280

tgggcggttt tatggacagc aagcgaaccg gaattgccag ctggggcgcc ctctggtaag     2340

gttgggaagc cctgcaaagt aaactggatg gctttctcgc cgccaaggat ctgtcgaccc     2400

ctagatttca gtgcaattta tctcttcaaa tgtagcacct gaagtcagcc ccatacgata     2460

taagttgttg gaagatctag cccgcctaat gagcgggctt ttttttaatt cgcaattccc     2520

cgatgcataa tgtgcctgtc aaatggacga agcagggatt ctgcaaaccc tatgctactc     2580

cgtcaagccg tcaattgtct gattcgttac caattatgac aacttgacgg ctacatcatt     2640

cactttttct tcacaaccgg cacgaaactc gctcgggctg gccccggtgc attttttaaa     2700

tactcgcgag aaatagagtt gatcgtcaaa accaacattg cgaccgacgg tggcgatagg     2760

catccgggta gtgctcaaaa gcagcttcgc ctgactaatg cgttggtcct cgcgccagct     2820

taagacgcta atccctaact gctggcggaa aagatgtgac agacgcgacg gcgacaagca     2880

aacatgctgt gcgacgctgg cgatatcaaa attgctgtct gccaggtgat cgctgatgta     2940

ctgacaagcc tcgcgtaccc gattatccat cggtggatgg agcgactcgt taatcgcttc     3000

catgcgccgc agtaacaatt gctcaagcag atttatcgcc agcagctccg aatagcgccc     3060

ttccccttgc ccggcgttaa tgatttgccc aaacaggtcg ctgaaatgcg gctggtgcgc     3120

ttcatccggg cgaaagaaac ccgtattggc aaatattgac ggccagttaa gccattcatg     3180

ccagtaggcg cgcggacgaa agtaaaccca ctggtgatac cattcgcgag cctccggatg     3240

acgaccgtag tgatgaatct ctcctggcgg gaacagcaaa atatcacccg gtcggcagac     3300

aaattctcgt ccctgatttt tcaccacccc ctgaccgcga atggtgagat tgagaatata     3360

acctttcatt cccagcggtc ggtcgataaa aaaatcgaga taaccgttgg cctcaatcgg     3420

cgttaaaccc gccaccagat gggcgttaaa cgagtatccc ggcagcaggg gatcattttg     3480

cgcttcagcc atacttttca tactcccacc attcagagaa gaaaccaatt gtccatattg     3540

catcagacat tgccgtcact gcgtctttta ctggctcttc tcgctaaccc aaccggtaac     3600

cccgcttatt aaaagcattc tgtaacaaag cgggaccaaa gccatgacaa aaacgcgtaa     3660

caaaagtgtc tataatcacg gcagaaaagt ccacattgat tatttgcacg gcgtcacact     3720

ttgctatgcc atagcatttt tatccataag attagcggat cctacctgac gctttttatc     3780

gcaactctct actgtttctc catacccgtt tttttgggct agcgaattct gagaacaaac     3840

taagtggata aatttcgtgt tcaggggcca acgaagctcc agggcgaagt cacaatttcc     3900

ggcgctaaaa atgctgctct gcctatcctt tttgccgcac tactggcgga agaaccggta     3960

gagatccaga acgtcccgaa actgaaagac gtcgatacat caatgaagct gctaagccag     4020

ctgggtgcga aagtagaacg taatggttct gtgcatattg atgcccgcga cgttaatgta     4080

ttctgcgcac cttacgatct ggttaaaacc atgcgtgctt ctatctgggc gctggggccg     4140

ctggtagcgc gctttggtca ggggcaagtt tcactacctg gcggttgtac gatcggtgcg     4200

cgtccggttg atctacacat ttctggcctc gaacaattag gcgcgaccat caaactggaa     4260

gaaggttacg ttaaagcttc cgtcgatggt cgtttgaaag gtgcacatat cgtgatggat     4320

aaagtcagcg ttggcgcaac ggtgaccatc atgtgtgctg caaccctggc ggaaggcacc     4380

acgattattg aaaacgcagc gcgtgaaccg gaaatcgtcg ataccgcgaa cttcctgatt     4440

acgctgggtg cgaaaattag cggtcagggc accgatcgta tcgtcatcga aggtgtggaa     4500

cgtttaggcg gcggtgtcta tcgcgttctg ccggatcgta tcgaaaccgg tactttcctg     4560

gtggcggcgg cgatttctcg cggcaaaatt atctgccgta acgcgcagcc agatactctc     4620

gacgccgtgc tggcgaaact gcgtgacgct ggagcggaca tcgaagtcgg cgaagactgg     4680

attagcctgg atatgcatgg caaacgtccg aaggctgtta acgtacgtac cgcgccgcat     4740

ccggcattcc cgaccgatat gcaggcccag ttcacgctgt tgaacctggt ggcagaaggg     4800

accgggttta tcaccgaaac ggtctttgaa aaccgcttta tgcatgtgcc agagctgagc     4860

cgtatgggcg cgcacgccga aatcgaaagc aataccgtta tttgtcacgg tgttgaaaaa     4920

ctttctggcg cacaggttat ggcaaccgat ctgcgtgcat cagcaagcct ggtgctggct     4980

ggctgtattg cggaagggac gacggtggtt gatcgtattt atcacatcga tcgtggctac     5040

gaacgcattg aagacaaact gcgcgcttta ggtgcaaata ttgagcgtgt gaaaggcgaa     5100

taagaattca ggaaaaaaac gctgtgaaaa atgttggttt tatcggctgg cgcggaatgg     5160

tcggctctgt tctcatgcaa cgcatggtag aggagcgcga tttcgacgct attcgccctg     5220

ttttcttttc tacctcccag tttggacagg cggcgcccac cttcggcgac acctccaccg     5280

gcacgctaca ggacgctttt gatctggatg cgctaaaagc gctcgatatc atcgtgacct     5340

gccagggcgg cgattatacc aacgaaattt atccaaagct gcgcgaaagc ggatggcagg     5400

gttactggat tgatgcggct tctacgctgc gcatgaaaga tgatgccatt attattctcg     5460

acccggtcaa ccaggacgtg attaccgacg gcctgaacaa tggcgtgaag acctttgtgg     5520

gcggtaactg taccgttagc ctgatgttga tgtcgctggg cggtctcttt gcccataatc     5580

tcgttgactg ggtatccgtc gcgacctatc aggccgcctc cggcggcggc gcgcgccata     5640

tgcgcgagct gttaacccag atgggtcagt tgtatggcca tgtcgccgat gaactggcga     5700

cgccgtcttc cgcaattctt gatattgaac gcaaagttac ggcattgacc cgcagcggcg     5760

agctgccggt tgataacttt ggcgtaccgc tggcgggaag cctgatcccc tggatcgaca     5820

aacagctcga taacggccag agccgcgaag agtggaaagg ccaggcggaa accaacaaga     5880

ttctcaatac tgcctctgtg attccggttg atggtttgtg tgtgcgcgtc ggcgcgctgc     5940

gctgtcacag ccaggcgttc accatcaagc tgaaaaaaga ggtatccatt ccgacggtgg     6000

aagaactgct ggcggcacat aatccgtggg cgaaagtggt gccgaacgat cgtgatatca     6060

ctatgcgcga attaaccccg gcggcggtga ccggcacgtt gactacgccg gttggtcgtc     6120

tgcgtaagct gaacatgggg ccagagttct tgtcggcgtt taccgtaggc gaccagttgt     6180

tatggggcgc cgccgagccg ctgcgtcgaa tgctgcgcca gttggcgtag tctagctgca     6240

cgataccgtc gacttgtaca tagactcgct ccgaaattaa agaacactta aattatctac     6300

taaaggaatc tttagtcaag tttatttaag atgacttaac tatgaataca caattgatgg     6360

gtgagcgtag gatcttccat tattgaagca tttatcaggg ttattgtctc atgagcttgg     6420

ctgttttggc ggatgagaga agattttcag cctgatacag attaaatcag aacgcagaag     6480

cggtctgata aaacagtttg cctggcggca gtagcgcggt ggtcccacct gaccccatgc     6540

cgaactcaga agtgaaacgc cgtagcgccg atggtagtgt ggggtctccc catgcgagag     6600

tagggaactg ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt     6660

tttatctgtt gtttgtcggt gaacgctctc ctgagtagga caaatccgcc gggagcggat     6720

ttgaacgttg cgaagcaacg gcccggaggg tggcgggcag gacgcccgcc ataaactgcc     6780

aggcatcaaa ttaagcagaa ggccatcctg acggatggcc tttttgcgtt tctacaaact     6840

ctttttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct     6900

gataaatgct tcaataatgg aagatcttcc aacatcacag gtaaacagaa acgtcgggtc     6960

gatcgggaaa ttctttcccg gacggcgcgg ggttgggcaa gccgcaggcg cgtcagtgct     7020

tttagcgggt gtcggggcag ccctgaacca gtcacgggat cgatctgtgc ggtatttcac     7080

accgcataca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt     7140

ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata     7200

atagcacgtg ctaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga     7260

taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt     7320

agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca     7380

aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct     7440

ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta     7500

gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct     7560

aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc     7620

aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca     7680

gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga     7740

aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg     7800

aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt     7860

cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag     7920

cctatggaaa aacgccagca acgcggcctt tttacggttc ctgggctttt gctggccttt     7980

tgctcacatg ttct                                                       7994


<210>  9
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- FZD NcoI Primer 1

<400>  9
cgccatggac cacggcttct gc                                                22


<210>  10
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- FZD XmaI Primer 2

<400>  10
gccccgggct attacggcgc gc                                                22


<210>  11
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- LRP6P NcoI Primer 1

<400>  11
cgccatgggc atcgtacccg aa                                                22


<210>  12
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- LRP6P XmaI Primer 2

<400>  12
ccccgggcta ttagcatgac aa                                                22


<210>  13
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- PD1 NcoI Primer 1

<400>  13
cgccatgggc ttagactccc ca                                                22


<210>  14
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- PD1 XmaI Primer 2

<400>  14
gccccgggct attagggtga gg                                                22


<210>  15
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- SPRI-alpha NcoI Primer 1

<400>  15
cgccatgggc gttgcgggcg aa                                                22


<210>  16
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- SPRI-alpha XmaI Primer 2

<400>  16
gccccgggct attaacgttc gt                                                22


<210>  17
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- FZD KpnI Primer 1

<400>  17
ggggtaccac caccatggac cacggcttct gccagcc                                37


<210>  18
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- FZD XhoI Primer 2

<400>  18
gccgctcgag ctattaggga gctccgtcct cggagt                                 36


<210>  19
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- LRP6P KpnI Primer 1

<400>  19
ggggtaccac catgggcatt gtcccagagg ctttcct                                37


<210>  20
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- LRP6P XhoI Primer 2

<400>  20
gccgctcgag ttcaagatga gctatcatgt taatag                                 36


<210>  21
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- PD1 KpnI Primer 1

<400>  21
ggggtaccac catgggctta gactccccag acaggcc                                37


<210>  22
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- PD1 XhoI Primer 2

<400>  22
gccgctcgag ctattagggt gaggggctgg ggtgg                                  35


<210>  23
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- SPRI-alpha KpnI Primer 1

<400>  23
ggggtaccac catgggagtg gcgggtgagg aggag                                  35


<210>  24
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- SPRI-alpha XhoI Primer 2

<400>  24
gccgctcgag ctattaccgt tcattagatc cagtgt                                 36


