                         SEQUENCE LISTING

<110>  Skysong Innovations
       Kong, Wei
 
<120>  TUMOR-NAVIGATING, SELF-ERADICATING, TRAIL-ARMED SALMONELLA FOR 
       PRECISION CANCER THERAPY

<130>  112624.01160

<150>  62/809,222
<151>  2019-02-22

<160>  44    

<170>  PatentIn version 3.5

<210>  1
<211>  94
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- Promoter: Ptrc lacO deletion

<400>  1
attctgaaat gagctgttga caattaatca tccggctcgt ataatgtgta gatgcgtagg       60

cacctgttac gacgaaccac acaggaaaca gacc                                   94


<210>  2
<211>  1756
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- tar deletion-insertion mutation

<400>  2
attctgaaat gagctgttga caattaatca tccggctcgt ataatgtgta gatgcgtagg       60

cacctgttac gacgaaccac acaggaaaca gaccatgttt aaccgtatcc gcgttgtcac      120

aatgctgatg atggtgctgg gggttttcgc actgctacag cttgtttccg gtggtttgct      180

gttttcttca ttacagcata accagcaagg ttttgttatt tctaacgaat tacgtcagca      240

acaaagcgaa ctcacgtcga catgggactt aatgctgcaa acgcgcatta acctgagccg      300

ctccgccgca cgcatgatga tggacgcttc taaccagcag agcagcgcca aaacggattt      360

actccagaat gcaaaaacga ctctcgcaca ggcggcggcg cactacgcca atttcaaaaa      420

tatgacgcca ttgccagcga tggcggaggc cagcgcgaac gtcgatgaaa aatatcagcg      480

ctatcaggcc gcattagccg aacttattca gtttctggac aatggcaata tggatgccta      540

cttcgcccag ccaacccagg gaatgcaaaa cgcgttgggc gaggcgctgg gcaattacgc      600

ccgggtgagc gaaaacctgt accgccagac atttgatcaa agtgctcatg actaccgttt      660

tgcgcaatgg caactggggg ttcttgcggt cgtgctggtg ctgattttga tggtggtttg      720

gttcggcatt cgtcatgccc tgcttaaccc attagcgcga gtgattactc atatccgtga      780

aattgccagc ggcgatctga cgaaaacgct caccgtctca ggacgtaatg aaattggcga      840

actggcggga acggttgaac atatgcaacg ctcgctgatt gacaccgtaa cgcaggttcg      900

tgaaggttcg gatgcgattt attccggcac cagtgaaatt gccgccggta ataccgacct      960

ctcttcccgt accgaacagc aggcctccgc tctggaggag acggctgcca gcatggaaca     1020

actgacggcc accgtgaagc aaaacgccga taacgcccgc caggcttcgc aactggcgca     1080

aagcgcctcc gagaccgcgc gtcatggcgg caaagtggtc gacggcgtag taaacactat     1140

gcacgaaatt gccgacagtt cgaaaaaaat cgctgacatt atcagcgtta tcgacggtat     1200

tgccttccag actaacattc tggcgctgaa cgcggcggta gaagcggcgc gcgcgggaga     1260

gcaggggcgc ggttttgcgg tcgtggcagg cgaggtgcgt aatctggcca gccgcagcgc     1320

ccaggcggcg aaagaaataa aagcgttgat tgaagattcc gtctcgcgtg tcgataccgg     1380

ttctgtgctg gtggaaagcg ccggggaaac catgactgac atcgtcaatg ccgttacgcg     1440

cgtcacggat atcatgggcg aaatcgcctc cgcctcggat gagcaaagcc ggggtatcga     1500

tcaggtcgct ttggccgttt ccgaaatgga tcgcgtaacg caacagaacg cctcgctggt     1560

tcaggaatcc gcagcggccg ccgccgcgct ggaagagcag gccagccgtc tgacccaggc     1620

ggtatcggct ttccgcctgg catcgcgacc gctggcggta aataaacctg agatgcgttt     1680

gtcagtgaac gctcagtccg gcaatacgcc gcagtcatta gccgccaggg atgatgcgaa     1740

ctgggaaacc ttctga                                                     1756


<210>  3
<211>  6177
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- plasmid pK4946: suicide vector for construction of tar
       deletion-insertion mutation

<400>  3
gatccttttt aacccatcac atatacctgc cgttcactat tatttagtga aatgagatat       60

tatgatattt tctgaattgt gattaaaaag gcaactttat gcccatgcaa cagaaactat      120

aaaaaataca gagaatgaaa agaaacagat agatttttta gttctttagg cccgtagtct      180

gcaaatcctt ttatgatttt ctatcaaaca aaagaggaaa atagaccagt tgcaatccaa      240

acgagagtct aatagaatga ggtcgaaaag taaatcgcgc gggtttgtta ctgataaagc      300

aggcaagacc taaaatgtgt aaagggcaaa gtgtatactt tggcgtcacc ccttacatat      360

tttaggtctt tttttattgt gcgtaactaa cttgccatct tcaaacagga gggctggaag      420

aagcagaccg ctaacacagt acataaaaaa ggagacatga acgatgaaca tcaaaaagtt      480

tgcaaaacaa gcaacagtat taacctttac taccgcactg ctggcaggag gcgcaactca      540

agcgtttgcg aaagaaacga accaaaagcc atataaggaa acatacggca tttcccatat      600

tacacgccat gatatgctgc aaatccctga acagcaaaaa aatgaaaaat atcaagttcc      660

tgagttcgat tcgtccacaa ttaaaaatat ctcttctgca aaaggcctgg acgtttggga      720

cagctggcca ttacaaaacg ctgacggcac tgtcgcaaac tatcacggct accacatcgt      780

ctttgcatta gccggagatc ctaaaaatgc ggatgacaca tcgatttaca tgttctatca      840

aaaagtcggc gaaacttcta ttgacagctg gaaaaacgct ggccgcgtct ttaaagacag      900

cgacaaattc gatgcaaatg attctatcct aaaagaccaa acacaagaat ggtcaggttc      960

agccacattt acatctgacg gaaaaatccg tttattctac actgatttct ccggtaaaca     1020

ttacggcaaa caaacactga caactgcaca agttaacgta tcagcatcag acagctcttt     1080

gaacatcaac ggtgtagagg attataaatc aatctttgac ggtgacggaa aaacgtatca     1140

aaatgtacag cagttcatcg atgaaggcaa ctacagctca ggcgacaacc atacgctgag     1200

agatcctcac tacgtagaag ataaaggcca caaatactta gtatttgaag caaacactgg     1260

aactgaagat ggctaccaag gcgaagaatc tttatttaac aaagcatact atggcaaaag     1320

cacatcattc ttccgtcaag aaagtcaaaa acttctgcaa agcgataaaa aacgcacggc     1380

tgagttagca aacggcgctc tcggtatgat tgagctaaac gatgattaca cactgaaaaa     1440

agtgatgaaa ccgctgattg catctaacac agtaacagat gaaattgaac gcgcgaacgt     1500

ctttaaaatg aacggcaaat ggtatctgtt cactgactcc cgcggatcaa aaatgacgat     1560

tgacggcatt acgtctaacg atatttacat gcttggttat gtttctaatt ctttaactgg     1620

cccatacaag ccgctgaaca aaactggcct tgtgttaaaa atggatcttg atcctaacga     1680

tgtaaccttt acttactcac acttcgctgt acctcaagcg aaaggaaaca atgtcgtgat     1740

tacaagctat atgacaaaca gaggattcta cgcagacaaa caatcaacgt ttgcgcccag     1800

cttcctgctg aacatcaaag gcaagaaaac atctgttgtc aaagacagca tccttgaaca     1860

aggacaatta acagttaaca aataaaaacg caaaagaaaa tgccgatatc ctattggcat     1920

tttcttttat ttcttatcaa cataaaggtg aatcccatat gaactatata aaagcaggca     1980

aatggctaac cgtattccta accttttgaa ttcgatcgct agtttgtttt gactccatcc     2040

attagggctt ctaaaacgcc ttctaaggcc atgtcagccg ttaagtgttc ctgtgtcact     2100

gaaaattgct ttgagaggct ctaagggctt ctcagtgcgt tacatccctg gcttgttgtc     2160

cacaaccgtt aaaccttaaa agctttaaaa gccttatata ttcttttttt tcttataaaa     2220

cttaaaacct tagaggctat ttaagttgct gatttatatt aattttattg ttcaaacatg     2280

agagcttagt acgtgaaaca tgagagctta gtacgttagc catgagagct tagtacgtta     2340

gccatgaggg tttagttcgt taaacatgag agcttagtac gttaaacatg agagcttagt     2400

acgtgaaaca tgagagctta gtacgtacta tcaacaggtt gaactgctgg atcgatcctt     2460

tttgtccggt gttgggttga aggtgaagcc ggtcggggcc gcagcggggg ccggcttttc     2520

agccttgccc ccctgcttcg gccgccgtgg ctccggcgtc ttgggtgccg gcgcgggttc     2580

cgcagccttg gcctgcggtg cgggcacatc ggcgggcttg gccttgatgt gccgcctggc     2640

gtgcgagcgg aacgtctcgt aggagaactt gaccttcccc gtttcccgca tgtgctccca     2700

aatggtgacg agcgcatagc cggacgctaa cgccgcctcg acatccgccc tcaccgccag     2760

gaacgcaacc gcagcctcat cacgccggcg cttcttggcc gcgcgggatt caacccactc     2820

ggccagctcg tcggtgtagc tctttggcat cgtctctcgc ctgtcccctc agttcagtaa     2880

tttcctgcat ttgcctgttt ccagtcggta gatattccac aaaacagcag ggaagcagcg     2940

cttttccgct gcataaccct gcttcggggt cattatagcg attttttcgg tatatccatc     3000

ctttttcgca cgatatacag gattttgcca aagggttcgt gtagactttc cttggtgtat     3060

ccaacggcgt cagccgggca ggataggtga agtaggccca cccgcgagcg ggtgttcctt     3120

cttcactgtc ccttattcgc acctggcggt gctcaacggg aatcctgctc tgcgaggctg     3180

gccggctacc gccggcgtaa cagatgaggg caagcggatg gctgatgaaa ccaagccaac     3240

caggaagggc agcccaccta tcaaggtgta ctgccttcca gacgaacgaa gagcgattga     3300

ggaaaaggcg gcggcggccg gcatgagcct gtcggcctac ctgctggccg tcggccaggg     3360

ctacaaaatc acgggcgtcg tggactatga gcacgtccgc gagctggccc gcatcaatgg     3420

cgacctgggc cgcctgggcg gcctgctgaa actctggctc accgacgacc cgcgcacggc     3480

gcggttcggt gatgccacga tcctcgccct gctggcgaag atcgaagaga agcaggacga     3540

gcttggcaag gtcatgatgg gcgtggtccg cccgagggca gagccatgac ttttttagcc     3600

gctaaaacgg ccggggggtg cgcgtgattg ccaagcacgt ccccatgcgc tccatcaaga     3660

agagcgactt cgcggagctg gtgaagtaca tcaccgacga gcaaggcaag accgagcgcc     3720

tgggtcacgt gcgcgtcacg aactgcgagg caaacaccct gcccgctgtc atggccgagg     3780

tgatggcgac ccagcacggc aacacccgtt ccgaggccga caagacctat cacctgctgg     3840

ttagcttccg cgcgggagag aagcccgacg cggagacgtt gcgcgcgatt gaggaccgca     3900

tctgcgctgg gcttggcttc gccgagcatc agcgcgtcag tgccgtgcat cacgacaccg     3960

acaacctgca catccatatc gccatcaaca agattcaccc gacccgaaac accatccatg     4020

agccgtatcg ggcctaccgc gccctcgctg acctctgcgc gacgctcgaa cgggactacg     4080

ggcttgagcg tgacaatcac gaaacgcggc agcgcgtttc cgagaaccgc gcgaacgaca     4140

tggagcggca cgcgggcgtg gaaagcctgg tcggctggat cgggccctaa atacctgtga     4200

cggaagatca cttcgcagaa taaataaatc ctggtgtccc tgttgatacc gggaagccct     4260

gggccaactt ttggcgaaaa tgagacgttg atcggcacgt aagaggttcc aactttcacc     4320

ataatgaaat aagatcacta ccgggcgtat tttttgagtt atcgagattt tcaggagcta     4380

aggaagctaa aatggagaaa aaaatcactg gatataccac cgttgatata tcccaatggc     4440

atcgtaaaga acattttgag gcatttcagt cagttgctca atgtacctat aaccagaccg     4500

ttcagctgga tattacggcc tttttaaaga ccgtaaagaa aaataagcac aagttttatc     4560

cggcctttat tcacattctt gcccgcctga tgaatgctca tccggaattc cgtatggcaa     4620

tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc gttttccatg     4680

agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc cggcagtttc     4740

tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag     4800

ggtttattga gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc accagttttg     4860

atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg ggcaaatatt     4920

atacgcaagg cgacaaggtg ctgatgccgc tggcgattca ggttcatcat gccgtctgtg     4980

atggcttcca tgtcggcaga atgcttaatg aattacaaca gtactgcgat gagtggcagg     5040

gcggggcgta atttttttaa ggcagttatt ggtgccctta aacgcctggt gctacgcctg     5100

aataagtgat agggcccgat cccaagcttc ttctagaggt accgacttca tcgccaatac     5160

accggccttt ataaaagggg tgactaacct gcgcggcgtg attgtcccca ttgtcgatct     5220

gcgcgtgaaa ttctgcgaag gcgacgttga gtacgatgac aatacggtag tgatcgtact     5280

gaatctgggg caacgcgtcg tcgggatagt ggtagacggc gtgtctgacg tactgtcgtt     5340

aacggcggaa cagatccgtc cggcgccaga atttgccgtg accttgtcaa cagaatattt     5400

gacgggattg ggcgcgctcg gcgagcgtat gctgattctg gtgaatatcg aaaaactgct     5460

taacagcgaa gagatggcgc tgctggatat cgcagcatca cacgtcgcgt aataacgttg     5520

ccggatggcg tcgcgccatc cggcaatatt caccgttatg cttccgccag cggtgagagt     5580

gcctcttcat cgcccgcgat tctgaaatga gctgttgaca attaatcatc cggctcgtat     5640

aatgtgtaga tgcgtaggca cctgttacga cgaaccacac aggaaacaga ccatgtttaa     5700

ccgtatccgc gttgtcacaa tgctgatgat ggtgctgggg gttttcgcac tgctacagct     5760

tgtttccggt ggtttgctgt tttcttcatt acagcataac cagcaaggtt ttgttatttc     5820

taacgaatta cgtcagcaac aaagcgaact cacgtcgaca tgggacttaa tgctgcaaac     5880

gcgcattaac ctgagccgct ccgccgcacg catgatgatg gacgcttcta accagcagag     5940

cagcgccaaa acggatttac tccagaatgc aaaaacgact ctcgcacagg cggcggcgca     6000

ctacgccaat ttcaaaaata tgacgccatt gccagcgatg gcggaggcca gcgcgaacgt     6060

cgatgaaaaa tatcagcgct atcaggccgc attagccgaa cttattcagt ttctggacaa     6120

tggcaatatg gatgcctact tcttgtcgca tgcgatatcg agctctcccg ggaattc        6177


<210>  4
<211>  1756
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- tsr deletion-insertion mutation

<400>  4
attctgaaat gagctgttga caattaatca tccggctcgt ataatgtgta gatgcgtagg       60

cacctgttac gacgaaccac acaggaaaca gaccatgtta aagcgaatta aaattgttac      120

cagcttactg ctggtattgg cgctatttgg ccttttacaa ctgacatccg gcgggctgtt      180

cttcaactcg ctgaagaatg acaaagaaaa cttcaccgta ttgcaaacta ttcgtcagca      240

gcagtctgcc ctgaatgcaa cctgggtgga gctgttgcaa acgcgtaata ccctgaatcg      300

cgcgggtatc cgctggatga tggaccagag caatattggc agcggcgcaa ctgtcgctga      360

actgatgcag ggggcgacca atacgctgaa gctgaccgaa aaaaactggg agcagtatga      420

ggcgttaccg cgcgatccac gtcagagtga agcggctttc cttgagatca aacgaaccta      480

tgatatctac cacggcgcgt tggcggagct tattcagctt cttggcgcgg gtaagattaa      540

cgagtttttt gatcaaccga ctcaaagcta tcaggacgct tttgagaagc agtacatggc      600

ctatatgcag caaaacgatc gtctgtacga tattgctgtt gaggataaca acagttccta      660

caaccaggcg atgtgggtac tggtcagtgt gctgattgcc gttctggtgg tcattatcgc      720

cgtctggttc ggcatcaaac tgtcgcttat cgccccgatg aatcgtctga ttgaaagcat      780

tcgtcatatc gccagcggcg atctggtgaa gcgtatcgac gtggaaggct ccaacgaaat      840

ggggcagttg gctgaaaacc tgcgtcatat gcaaagtgaa ctgatgcgta ccgtgggcga      900

tgtacgtaac ggcgcgaatg cgatctatag cggcgccagc gagattgcga tgggcaacaa      960

cgatctctct tcccgtactg agcagcaggc agcgtctctg gaagagaccg ccgccagtat     1020

ggaacaactg accgccaccg tgaaacagaa cgccgaaaac gcccgtcagg ccagtcacct     1080

ggcgctgagt gcgtcagaga cagcgcaaaa aggcggcaaa gtggtggata acgtcgtaca     1140

aacaatgcgc gatatcgcct ccagttcgca gaaaatcgcc gatattatca gcgtaatcga     1200

cggtattgct ttccagacca atattctggc gctgaatgcg gcggtagaag cggcgcgcgc     1260

aggcgagcag ggacgcgggt tcgcagtggt ggccggtgaa gtccgtaatc tggcccagcg     1320

tagcgcgcag gcggcacggg agatcaagag tctgattgag gattccgtga gccgtgttga     1380

tgtaggttcg acgctggtcg aaagcgccgg tgaaaccatg gatgagatcg tcaatgcagt     1440

gacccgcgtg accgatatca tgggcgagat tgcctcggcg tctgacgagc aaagccgtgg     1500

tatcgaccag gtgggcctgg cggtagcgga gatggatcgc gtaacgcagc agaacgcctc     1560

gctggtggaa gagtccgccg ccgcggctgc ggcgctggaa gagcaagcca gccgtctgac     1620

ccaggccgtc gcggtgttcc gtattcacca gcaacagcag cgtgcgcgtg aagtggctgc     1680

ggtaaaaacc ccggcagccg tgtcgtcacc aaaggccgca gtggccgacg gcagcgataa     1740

ttgggaaaca ttttaa                                                     1756


<210>  5
<211>  6177
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- plasmid pK4947: suicide vector for construction of tsr
       deletion-insertion mutation

<400>  5
gatccttttt aacccatcac atatacctgc cgttcactat tatttagtga aatgagatat       60

tatgatattt tctgaattgt gattaaaaag gcaactttat gcccatgcaa cagaaactat      120

aaaaaataca gagaatgaaa agaaacagat agatttttta gttctttagg cccgtagtct      180

gcaaatcctt ttatgatttt ctatcaaaca aaagaggaaa atagaccagt tgcaatccaa      240

acgagagtct aatagaatga ggtcgaaaag taaatcgcgc gggtttgtta ctgataaagc      300

aggcaagacc taaaatgtgt aaagggcaaa gtgtatactt tggcgtcacc ccttacatat      360

tttaggtctt tttttattgt gcgtaactaa cttgccatct tcaaacagga gggctggaag      420

aagcagaccg ctaacacagt acataaaaaa ggagacatga acgatgaaca tcaaaaagtt      480

tgcaaaacaa gcaacagtat taacctttac taccgcactg ctggcaggag gcgcaactca      540

agcgtttgcg aaagaaacga accaaaagcc atataaggaa acatacggca tttcccatat      600

tacacgccat gatatgctgc aaatccctga acagcaaaaa aatgaaaaat atcaagttcc      660

tgagttcgat tcgtccacaa ttaaaaatat ctcttctgca aaaggcctgg acgtttggga      720

cagctggcca ttacaaaacg ctgacggcac tgtcgcaaac tatcacggct accacatcgt      780

ctttgcatta gccggagatc ctaaaaatgc ggatgacaca tcgatttaca tgttctatca      840

aaaagtcggc gaaacttcta ttgacagctg gaaaaacgct ggccgcgtct ttaaagacag      900

cgacaaattc gatgcaaatg attctatcct aaaagaccaa acacaagaat ggtcaggttc      960

agccacattt acatctgacg gaaaaatccg tttattctac actgatttct ccggtaaaca     1020

ttacggcaaa caaacactga caactgcaca agttaacgta tcagcatcag acagctcttt     1080

gaacatcaac ggtgtagagg attataaatc aatctttgac ggtgacggaa aaacgtatca     1140

aaatgtacag cagttcatcg atgaaggcaa ctacagctca ggcgacaacc atacgctgag     1200

agatcctcac tacgtagaag ataaaggcca caaatactta gtatttgaag caaacactgg     1260

aactgaagat ggctaccaag gcgaagaatc tttatttaac aaagcatact atggcaaaag     1320

cacatcattc ttccgtcaag aaagtcaaaa acttctgcaa agcgataaaa aacgcacggc     1380

tgagttagca aacggcgctc tcggtatgat tgagctaaac gatgattaca cactgaaaaa     1440

agtgatgaaa ccgctgattg catctaacac agtaacagat gaaattgaac gcgcgaacgt     1500

ctttaaaatg aacggcaaat ggtatctgtt cactgactcc cgcggatcaa aaatgacgat     1560

tgacggcatt acgtctaacg atatttacat gcttggttat gtttctaatt ctttaactgg     1620

cccatacaag ccgctgaaca aaactggcct tgtgttaaaa atggatcttg atcctaacga     1680

tgtaaccttt acttactcac acttcgctgt acctcaagcg aaaggaaaca atgtcgtgat     1740

tacaagctat atgacaaaca gaggattcta cgcagacaaa caatcaacgt ttgcgcccag     1800

cttcctgctg aacatcaaag gcaagaaaac atctgttgtc aaagacagca tccttgaaca     1860

aggacaatta acagttaaca aataaaaacg caaaagaaaa tgccgatatc ctattggcat     1920

tttcttttat ttcttatcaa cataaaggtg aatcccatat gaactatata aaagcaggca     1980

aatggctaac cgtattccta accttttgaa ttcgatcgct agtttgtttt gactccatcc     2040

attagggctt ctaaaacgcc ttctaaggcc atgtcagccg ttaagtgttc ctgtgtcact     2100

gaaaattgct ttgagaggct ctaagggctt ctcagtgcgt tacatccctg gcttgttgtc     2160

cacaaccgtt aaaccttaaa agctttaaaa gccttatata ttcttttttt tcttataaaa     2220

cttaaaacct tagaggctat ttaagttgct gatttatatt aattttattg ttcaaacatg     2280

agagcttagt acgtgaaaca tgagagctta gtacgttagc catgagagct tagtacgtta     2340

gccatgaggg tttagttcgt taaacatgag agcttagtac gttaaacatg agagcttagt     2400

acgtgaaaca tgagagctta gtacgtacta tcaacaggtt gaactgctgg atcgatcctt     2460

tttgtccggt gttgggttga aggtgaagcc ggtcggggcc gcagcggggg ccggcttttc     2520

agccttgccc ccctgcttcg gccgccgtgg ctccggcgtc ttgggtgccg gcgcgggttc     2580

cgcagccttg gcctgcggtg cgggcacatc ggcgggcttg gccttgatgt gccgcctggc     2640

gtgcgagcgg aacgtctcgt aggagaactt gaccttcccc gtttcccgca tgtgctccca     2700

aatggtgacg agcgcatagc cggacgctaa cgccgcctcg acatccgccc tcaccgccag     2760

gaacgcaacc gcagcctcat cacgccggcg cttcttggcc gcgcgggatt caacccactc     2820

ggccagctcg tcggtgtagc tctttggcat cgtctctcgc ctgtcccctc agttcagtaa     2880

tttcctgcat ttgcctgttt ccagtcggta gatattccac aaaacagcag ggaagcagcg     2940

cttttccgct gcataaccct gcttcggggt cattatagcg attttttcgg tatatccatc     3000

ctttttcgca cgatatacag gattttgcca aagggttcgt gtagactttc cttggtgtat     3060

ccaacggcgt cagccgggca ggataggtga agtaggccca cccgcgagcg ggtgttcctt     3120

cttcactgtc ccttattcgc acctggcggt gctcaacggg aatcctgctc tgcgaggctg     3180

gccggctacc gccggcgtaa cagatgaggg caagcggatg gctgatgaaa ccaagccaac     3240

caggaagggc agcccaccta tcaaggtgta ctgccttcca gacgaacgaa gagcgattga     3300

ggaaaaggcg gcggcggccg gcatgagcct gtcggcctac ctgctggccg tcggccaggg     3360

ctacaaaatc acgggcgtcg tggactatga gcacgtccgc gagctggccc gcatcaatgg     3420

cgacctgggc cgcctgggcg gcctgctgaa actctggctc accgacgacc cgcgcacggc     3480

gcggttcggt gatgccacga tcctcgccct gctggcgaag atcgaagaga agcaggacga     3540

gcttggcaag gtcatgatgg gcgtggtccg cccgagggca gagccatgac ttttttagcc     3600

gctaaaacgg ccggggggtg cgcgtgattg ccaagcacgt ccccatgcgc tccatcaaga     3660

agagcgactt cgcggagctg gtgaagtaca tcaccgacga gcaaggcaag accgagcgcc     3720

tgggtcacgt gcgcgtcacg aactgcgagg caaacaccct gcccgctgtc atggccgagg     3780

tgatggcgac ccagcacggc aacacccgtt ccgaggccga caagacctat cacctgctgg     3840

ttagcttccg cgcgggagag aagcccgacg cggagacgtt gcgcgcgatt gaggaccgca     3900

tctgcgctgg gcttggcttc gccgagcatc agcgcgtcag tgccgtgcat cacgacaccg     3960

acaacctgca catccatatc gccatcaaca agattcaccc gacccgaaac accatccatg     4020

agccgtatcg ggcctaccgc gccctcgctg acctctgcgc gacgctcgaa cgggactacg     4080

ggcttgagcg tgacaatcac gaaacgcggc agcgcgtttc cgagaaccgc gcgaacgaca     4140

tggagcggca cgcgggcgtg gaaagcctgg tcggctggat cgggccctaa atacctgtga     4200

cggaagatca cttcgcagaa taaataaatc ctggtgtccc tgttgatacc gggaagccct     4260

gggccaactt ttggcgaaaa tgagacgttg atcggcacgt aagaggttcc aactttcacc     4320

ataatgaaat aagatcacta ccgggcgtat tttttgagtt atcgagattt tcaggagcta     4380

aggaagctaa aatggagaaa aaaatcactg gatataccac cgttgatata tcccaatggc     4440

atcgtaaaga acattttgag gcatttcagt cagttgctca atgtacctat aaccagaccg     4500

ttcagctgga tattacggcc tttttaaaga ccgtaaagaa aaataagcac aagttttatc     4560

cggcctttat tcacattctt gcccgcctga tgaatgctca tccggaattc cgtatggcaa     4620

tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc gttttccatg     4680

agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc cggcagtttc     4740

tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag     4800

ggtttattga gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc accagttttg     4860

atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg ggcaaatatt     4920

atacgcaagg cgacaaggtg ctgatgccgc tggcgattca ggttcatcat gccgtctgtg     4980

atggcttcca tgtcggcaga atgcttaatg aattacaaca gtactgcgat gagtggcagg     5040

gcggggcgta atttttttaa ggcagttatt ggtgccctta aacgcctggt gctacgcctg     5100

aataagtgat agggcccgat cccaagcttc ttctagaggt accgacttgt cgttattgat     5160

aaccgccggc gtcgcgcgcg tcgggtcgag tttcatgacc ttctgggcga tgtacaggct     5220

gtagtagcgg taagccacca gatagacgga aacggaagcg accacgatcc acagggcgct     5280

cacgtgttca ccccgacgta gggcaacgac cgagaggcag aaagcaccga tgattccgag     5340

aatcacccag ggtatgtgct tgaatatctt tttcgtatcc atagtaaaac ctggttgtaa     5400

gtttaattat cagccgaagc tgggtggaca ttgagttcgt gtttgaggag gtagattgac     5460

tgctatgctg agaggatctt gccagatcat cgcgcgcgta aagttaggta aatcagtgag     5520

tggttgtatg gcggtttaag cggtcggacg taccggtaag cggtttcgcg gaggagggcg     5580

tgggctattt tatgtgatat tctgaaatga gctgttgaca attaatcatc cggctcgtat     5640

aatgtgtaga tgcgtaggca cctgttacga cgaaccacac aggaaacaga ccatgttaaa     5700

gcgaattaaa attgttacca gcttactgct ggtattggcg ctatttggcc ttttacaact     5760

gacatccggc gggctgttct tcaactcgct gaagaatgac aaagaaaact tcaccgtatt     5820

gcaaactatt cgtcagcagc agtctgccct gaatgcaacc tgggtggagc tgttgcaaac     5880

gcgtaatacc ctgaatcgcg cgggtatccg ctggatgatg gaccagagca atattggcag     5940

cggcgcaact gtcgctgaac tgatgcaggg ggcgaccaat acgctgaagc tgaccgaaaa     6000

aaactgggag cagtatgagg cgttaccgcg cgatccacgt cagagtgaag cggctttcct     6060

tgagatcaaa cgaacctatg atatctacca cggcgcgttg gcggagctta ttcagcttct     6120

tggcgcgggt aagattaacg agttgtcgca tgcgatatcg agctctcccg ggaattc        6177


<210>  6
<211>  6176
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- plasmid pK4948: suicide vector for construction of trg
       deletion mutation

<400>  6
gatccttttt aacccatcac atatacctgc cgttcactat tatttagtga aatgagatat       60

tatgatattt tctgaattgt gattaaaaag gcaactttat gcccatgcaa cagaaactat      120

aaaaaataca gagaatgaaa agaaacagat agatttttta gttctttagg cccgtagtct      180

gcaaatcctt ttatgatttt ctatcaaaca aaagaggaaa atagaccagt tgcaatccaa      240

acgagagtct aatagaatga ggtcgaaaag taaatcgcgc gggtttgtta ctgataaagc      300

aggcaagacc taaaatgtgt aaagggcaaa gtgtatactt tggcgtcacc ccttacatat      360

tttaggtctt tttttattgt gcgtaactaa cttgccatct tcaaacagga gggctggaag      420

aagcagaccg ctaacacagt acataaaaaa ggagacatga acgatgaaca tcaaaaagtt      480

tgcaaaacaa gcaacagtat taacctttac taccgcactg ctggcaggag gcgcaactca      540

agcgtttgcg aaagaaacga accaaaagcc atataaggaa acatacggca tttcccatat      600

tacacgccat gatatgctgc aaatccctga acagcaaaaa aatgaaaaat atcaagttcc      660

tgagttcgat tcgtccacaa ttaaaaatat ctcttctgca aaaggcctgg acgtttggga      720

cagctggcca ttacaaaacg ctgacggcac tgtcgcaaac tatcacggct accacatcgt      780

ctttgcatta gccggagatc ctaaaaatgc ggatgacaca tcgatttaca tgttctatca      840

aaaagtcggc gaaacttcta ttgacagctg gaaaaacgct ggccgcgtct ttaaagacag      900

cgacaaattc gatgcaaatg attctatcct aaaagaccaa acacaagaat ggtcaggttc      960

agccacattt acatctgacg gaaaaatccg tttattctac actgatttct ccggtaaaca     1020

ttacggcaaa caaacactga caactgcaca agttaacgta tcagcatcag acagctcttt     1080

gaacatcaac ggtgtagagg attataaatc aatctttgac ggtgacggaa aaacgtatca     1140

aaatgtacag cagttcatcg atgaaggcaa ctacagctca ggcgacaacc atacgctgag     1200

agatcctcac tacgtagaag ataaaggcca caaatactta gtatttgaag caaacactgg     1260

aactgaagat ggctaccaag gcgaagaatc tttatttaac aaagcatact atggcaaaag     1320

cacatcattc ttccgtcaag aaagtcaaaa acttctgcaa agcgataaaa aacgcacggc     1380

tgagttagca aacggcgctc tcggtatgat tgagctaaac gatgattaca cactgaaaaa     1440

agtgatgaaa ccgctgattg catctaacac agtaacagat gaaattgaac gcgcgaacgt     1500

ctttaaaatg aacggcaaat ggtatctgtt cactgactcc cgcggatcaa aaatgacgat     1560

tgacggcatt acgtctaacg atatttacat gcttggttat gtttctaatt ctttaactgg     1620

cccatacaag ccgctgaaca aaactggcct tgtgttaaaa atggatcttg atcctaacga     1680

tgtaaccttt acttactcac acttcgctgt acctcaagcg aaaggaaaca atgtcgtgat     1740

tacaagctat atgacaaaca gaggattcta cgcagacaaa caatcaacgt ttgcgcccag     1800

cttcctgctg aacatcaaag gcaagaaaac atctgttgtc aaagacagca tccttgaaca     1860

aggacaatta acagttaaca aataaaaacg caaaagaaaa tgccgatatc ctattggcat     1920

tttcttttat ttcttatcaa cataaaggtg aatcccatat gaactatata aaagcaggca     1980

aatggctaac cgtattccta accttttgaa ttcgatcgct agtttgtttt gactccatcc     2040

attagggctt ctaaaacgcc ttctaaggcc atgtcagccg ttaagtgttc ctgtgtcact     2100

gaaaattgct ttgagaggct ctaagggctt ctcagtgcgt tacatccctg gcttgttgtc     2160

cacaaccgtt aaaccttaaa agctttaaaa gccttatata ttcttttttt tcttataaaa     2220

cttaaaacct tagaggctat ttaagttgct gatttatatt aattttattg ttcaaacatg     2280

agagcttagt acgtgaaaca tgagagctta gtacgttagc catgagagct tagtacgtta     2340

gccatgaggg tttagttcgt taaacatgag agcttagtac gttaaacatg agagcttagt     2400

acgtgaaaca tgagagctta gtacgtacta tcaacaggtt gaactgctgg atcgatcctt     2460

tttgtccggt gttgggttga aggtgaagcc ggtcggggcc gcagcggggg ccggcttttc     2520

agccttgccc ccctgcttcg gccgccgtgg ctccggcgtc ttgggtgccg gcgcgggttc     2580

cgcagccttg gcctgcggtg cgggcacatc ggcgggcttg gccttgatgt gccgcctggc     2640

gtgcgagcgg aacgtctcgt aggagaactt gaccttcccc gtttcccgca tgtgctccca     2700

aatggtgacg agcgcatagc cggacgctaa cgccgcctcg acatccgccc tcaccgccag     2760

gaacgcaacc gcagcctcat cacgccggcg cttcttggcc gcgcgggatt caacccactc     2820

ggccagctcg tcggtgtagc tctttggcat cgtctctcgc ctgtcccctc agttcagtaa     2880

tttcctgcat ttgcctgttt ccagtcggta gatattccac aaaacagcag ggaagcagcg     2940

cttttccgct gcataaccct gcttcggggt cattatagcg attttttcgg tatatccatc     3000

ctttttcgca cgatatacag gattttgcca aagggttcgt gtagactttc cttggtgtat     3060

ccaacggcgt cagccgggca ggataggtga agtaggccca cccgcgagcg ggtgttcctt     3120

cttcactgtc ccttattcgc acctggcggt gctcaacggg aatcctgctc tgcgaggctg     3180

gccggctacc gccggcgtaa cagatgaggg caagcggatg gctgatgaaa ccaagccaac     3240

caggaagggc agcccaccta tcaaggtgta ctgccttcca gacgaacgaa gagcgattga     3300

ggaaaaggcg gcggcggccg gcatgagcct gtcggcctac ctgctggccg tcggccaggg     3360

ctacaaaatc acgggcgtcg tggactatga gcacgtccgc gagctggccc gcatcaatgg     3420

cgacctgggc cgcctgggcg gcctgctgaa actctggctc accgacgacc cgcgcacggc     3480

gcggttcggt gatgccacga tcctcgccct gctggcgaag atcgaagaga agcaggacga     3540

gcttggcaag gtcatgatgg gcgtggtccg cccgagggca gagccatgac ttttttagcc     3600

gctaaaacgg ccggggggtg cgcgtgattg ccaagcacgt ccccatgcgc tccatcaaga     3660

agagcgactt cgcggagctg gtgaagtaca tcaccgacga gcaaggcaag accgagcgcc     3720

tgggtcacgt gcgcgtcacg aactgcgagg caaacaccct gcccgctgtc atggccgagg     3780

tgatggcgac ccagcacggc aacacccgtt ccgaggccga caagacctat cacctgctgg     3840

ttagcttccg cgcgggagag aagcccgacg cggagacgtt gcgcgcgatt gaggaccgca     3900

tctgcgctgg gcttggcttc gccgagcatc agcgcgtcag tgccgtgcat cacgacaccg     3960

acaacctgca catccatatc gccatcaaca agattcaccc gacccgaaac accatccatg     4020

agccgtatcg ggcctaccgc gccctcgctg acctctgcgc gacgctcgaa cgggactacg     4080

ggcttgagcg tgacaatcac gaaacgcggc agcgcgtttc cgagaaccgc gcgaacgaca     4140

tggagcggca cgcgggcgtg gaaagcctgg tcggctggat cgggccctaa atacctgtga     4200

cggaagatca cttcgcagaa taaataaatc ctggtgtccc tgttgatacc gggaagccct     4260

gggccaactt ttggcgaaaa tgagacgttg atcggcacgt aagaggttcc aactttcacc     4320

ataatgaaat aagatcacta ccgggcgtat tttttgagtt atcgagattt tcaggagcta     4380

aggaagctaa aatggagaaa aaaatcactg gatataccac cgttgatata tcccaatggc     4440

atcgtaaaga acattttgag gcatttcagt cagttgctca atgtacctat aaccagaccg     4500

ttcagctgga tattacggcc tttttaaaga ccgtaaagaa aaataagcac aagttttatc     4560

cggcctttat tcacattctt gcccgcctga tgaatgctca tccggaattc cgtatggcaa     4620

tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc gttttccatg     4680

agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc cggcagtttc     4740

tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag     4800

ggtttattga gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc accagttttg     4860

atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg ggcaaatatt     4920

atacgcaagg cgacaaggtg ctgatgccgc tggcgattca ggttcatcat gccgtctgtg     4980

atggcttcca tgtcggcaga atgcttaatg aattacaaca gtactgcgat gagtggcagg     5040

gcggggcgta atttttttaa ggcagttatt ggtgccctta aacgcctggt gctacgcctg     5100

aataagtgat agggcccgat cccaagcttc ttctagaggt accgacttga cgttattgtt     5160

gagcttactg atggcggcgt agatttcagt tttgaatgta tcggcaacgt taacgtgatg     5220

cgagcagcgc tggaatgttg tcataaaggc tggggcgaaa gcattattat tggcgtagcg     5280

ggggcagggc aggaaatcaa aacccgtccc ttccagctag tgaccggtcg cgtctggcgt     5340

ggctccgcct ttggcggcgt gaaagggcgc acgcagctgc cagggatggt cgaagatgcg     5400

atgaacggta aaatccgttt agatcctttt attactcacc gcctgccgct ggagcagatt     5460

aacgatgcct ttgaactgat gcatcagggt aaatccatcc gtactgttat ccactttggc     5520

gataactgat tcatccgcca gcggattttc cgctggcgct tttctgaatt ttctggaatg     5580

aatgttgtga aaatgtgatt ctgaaatgag ctgttgacaa ttaatcatcc ggctcgtata     5640

atgtgtagat gcgtaggcac ctgttacgac gaaccacaca ggaaacagac ctaagcgtcg     5700

cgccagcggg ctattttagc cgcgggcgcg tttattctcc gacagccgct atcacagcgg     5760

catcgattta cggatcgccg caatcagcat ttgcgccccg gttgagagcg ggatatcaac     5820

tcgcgtaaga atacctatcg gctcgcccgc gctctgggtc ggcacgggta atgagaccag     5880

cgtcgcctgg cgaagatctt ctttaaccgc gccagagggg acgaaccaga cgtaatcgta     5940

atctacggtc agttgtcgtg ataacgatgc ggatagcgtt tcgatacatc cggcaggcat     6000

tttacacccc tggctttgca acagggcttc cgcattctgg cgcggtaccg tgccttttgg     6060

cgagacgact accggccatt ccataacccg gctgagcgtg atcgtctcct gtaacagggg     6120

atgaccgggg cgtaccacta attgtcgcat gcgatatcga gctctcccgg gaattc         6176


<210>  7
<211>  6648
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- plasmid pK5079: lysis vector expressing human TRAIL

<400>  7
agatctagcc cgcctaatga gcgggctttt ttttaattcg caattccccg atgcataatg       60

tgcctgtcaa atggacgaag cagggattct gcaaacccta tgctactccg tcaagccgtc      120

aattgtctga ttcgttacca attatgacaa cttgacggct acatcattca ctttttcttc      180

acaaccggca cggaactcgc tcgggctggc cccggtgcat tttttaaata cccgcgagaa      240

atagagttga tcgtcaaaac caacattgcg accgacggtg gcgataggca tccgggtggt      300

gctcaaaagc agcttcgcct ggctgatacg ttggtcctcg cgccagctta agacgctaat      360

ccctaactgc tggcggaaaa gatgtgacag acgcgacggc gacaagcaaa catgctgtgc      420

gacgctggcg atatcaaaat tgctgtctgc caggtgatcg ctgatgtact gacaagcctc      480

gcgtacccga ttatccatcg gtggatggag cgactcgtta atcgcttcca tgcgccgcag      540

taacaattgc tcaagcagat ttatcgccag cagctccgaa tagcgccctt ccccttgccc      600

ggcgttaatg atttgcccaa acaggtcgct gaaatgcggc tggtgcgctt catccgggcg      660

aaagaacccc gtattggcaa atattgacgg ccagttaagc cattcatgcc agtaggcgcg      720

cggacgaaag taaacccact ggtgatacca ttcgcgagcc tccggatgac gaccgtagtg      780

atgaatctct cctggcggga acagcaaaat atcacccggt cggcaaacaa attctcgtcc      840

ctgatttttc accaccccct gaccgcgaat ggtgagattg agaatataac ctttcattcc      900

cagcggtcgg tcgataaaaa aatcgagata accgttggcc tcaatcggcg ttaaacccgc      960

caccagatgg gcattaaacg agtatcccgg cagcagggga tcattttgcg cttcagccat     1020

acttttcata ctcccgccat tcagagaaga aaccaattgt ccatattgca tcagacattg     1080

ccgtcactgc gtcttttact ggctcttctc gctaaccaaa ccggtaaccc cgcttattaa     1140

aagcattctg taacaaagcg ggaccaaagc catgacaaaa acgcgtaaca aaagtgtcta     1200

taatcacggc agaaaagtcc acattgatta tttgcacggc gtcacacttt gctatgccat     1260

agcattttta tccataagat tagcggatcc tacctgacgc tttttatcgc aactctctac     1320

tgtttctcca tacccgtttt tttgggctag cgaattctga gaacaaacta aatggataaa     1380

tttcgtgttc aggggccaac gaagctccag ggcgaagtca caatttccgg cgctaaaaat     1440

gctgctctgc ctatcctttt tgccgcacta ctggcggaag aaccggtaga gatccagaac     1500

gtcccgaaac tgaaagacgt cgatacatca atgaagctgc taagccagct gggtgcgaaa     1560

gtagaacgta atggttctgt gcatattgat gcccgcgacg ttaatgtatt ctgcgcacct     1620

tacgatctgg ttaaaaccat gcgtgcttct atctgggcgc tggggccgct ggtagcgcgc     1680

tttggtcagg ggcaagtttc actacctggc ggttgtacga tcggtgcgcg tccggttgat     1740

ctacacattt ctggcctcga acaattaggc gcgaccatca aactggaaga aggttacgtt     1800

aaagcttccg tcgatggtcg tttgaaaggt gcacatatcg tgatggataa agtcagcgtt     1860

ggcgcaacgg tgaccatcat gtgtgctgca accctggcgg aaggcaccac gattattgaa     1920

aacgcagcgc gtgaaccgga aatcgtcgat accgcgaact tcctgattac gctgggtgcg     1980

aaaattagcg gtcagggcac cgatcgtatc gtcatcgaag gtgtggaacg tttaggcggc     2040

ggtgtctatc gcgttctgcc ggatcgtatc gaaaccggta ctttcctggt ggcggcggcg     2100

atttctcgcg gcaaaattat ctgccgtaac gcgcagccag atactctcga cgccgtgctg     2160

gcgaaactgc gtgacgctgg agcggacatc gaagtcggcg aagactggat tagcctggat     2220

atgcatggca aacgtccgaa ggctgttaac gtacgtaccg cgccgcatcc ggcattcccg     2280

accgatatgc aggcccagtt cacgctgttg aacctggtgg cagaagggac cgggtttatc     2340

accgaaacgg tctttgaaaa ccgctttatg catgtgccag agctgagccg tatgggcgcg     2400

cacgccgaaa tcgaaagcaa taccgttatt tgtcacggtg ttgaaaaact ttctggcgca     2460

caggttatgg caaccgatct gcgtgcatca gcaagcctgg tgctggctgg ctgtattgcg     2520

gaagggacga cggtggttga tcgtatttat cacatcgatc gtggctacga acgcattgaa     2580

gacaaactgc gcgctttagg tgcaaatatt gagcgtgtga aaggcgaata agaattcagg     2640

aaaaaaacgc tgtgaaaaat gttggtttta tcggctggcg cggaatggtc ggctctgttc     2700

tcatgcaacg catggtagag gagcgcgatt tcgacgctat tcgccctgtt ttcttttcta     2760

cctcccagtt tggacaggcg gcgcccacct tcggcgacac ctccaccggc acgctacagg     2820

acgcttttga tctggatgcg ctaaaagcgc tcgatatcat cgtgacctgc cagggcggcg     2880

attataccaa cgaaatttat ccaaagctgc gcgaaagcgg atggcagggt tactggattg     2940

atgcggcttc tacgctgcgc atgaaagatg atgccattat tattctcgac ccggtcaacc     3000

aggacgtgat taccgacggc ctgaacaatg gcgtgaagac ctttgtgggc ggtaactgta     3060

ccgttagcct gatgttgatg tcgctgggcg gtctctttgc ccataatctc gttgactggg     3120

tatccgtcgc gacctatcag gccgcctccg gcggcggcgc gcgccatatg cgcgagctgt     3180

taacccagat gggtcagttg tatggccatg tcgccgatga actggcgacg ccgtcttccg     3240

caattcttga tattgaacgc aaagttacgg cattgacccg cagcggcgag ctgccggttg     3300

ataactttgg cgtaccgctg gcgggaagcc tgatcccctg gatcgacaaa cagctcgata     3360

acggccagag ccgcgaagag tggaaaggcc aggcggaaac caacaagatt ctcaatactg     3420

cctctgtgat tccggttgat ggtttgtgtg tgcgcgtcgg cgcgctgcgc tgtcacagcc     3480

aggcgttcac catcaagctg aaaaaagagg tatccattcc gacggtggaa gaactgctgg     3540

cggcacataa tccgtgggcg aaagtggtgc cgaacgatcg tgatatcact atgcgcgaat     3600

taaccccggc ggcggtgacc ggcacgttga ctacgccggt tggtcgtctg cgtaagctga     3660

acatggggcc agagttcttg tcggcgttta ccgtaggcga ccagttgtta tggggcgccg     3720

ccgagccgct gcgtcgaatg ctgcgccagt tggcgtagtc tagctgcacg ataccgtcga     3780

cttgtacata gactcgctcc gaaattaaag aacacttaaa ttatctacta aaggaatctt     3840

tagtcaagtt tatttaagat gacttaacta tgaatacaca attgatgggt gagcgtagga     3900

gcatgcttat gcgaaaggcc atcctgacgg atggcctttt tggatcttcc ggaagacctt     3960

ccattctgaa atgagctgtt gacaattaat catccggctc gtataatgtg tggaattgtg     4020

agcggataac aatttcacac aggaaacaga ccatggctat gatggaggtc caggggggac     4080

ccagcctggg acagacctgc gtgctgatcg tgatcttcac agtgctcctg cagtctctct     4140

gtgtggctgt aacttacgtg tactttacca acgagctgaa gcagatgcag gacaagtact     4200

ccaaaagtgg cattgcttgt ttcttaaaag aagatgacag ttattgggac cccaatgacg     4260

aagagagtat gaacagcccc tgctggcaag tcaagtggca actccgtcag ctcgttagaa     4320

agatgatttt gagaacctct gaggaaacca tttctacagt tcaagaaaag caacaaaata     4380

tttctcccct agtgagagaa agaggtcctc agagagtagc agctcacata actgggacca     4440

gaggaagaag caacacattg tcttctccaa actccaagaa tgaaaaggct ctgggccgca     4500

aaataaactc ctgggaatca tcaaggagtg ggcattcatt cctgagcaac ttgcacttga     4560

ggaatggtga actggtcatc catgaaaaag ggttttacta catctattcc caaacatact     4620

ttcgatttca ggaggaaata aaagaaaaca caaagaacga caaacaaatg gtccaatata     4680

tttacaaata cacaagttat cctgacccta tattgttgat gaaaagtgct agaaatagtt     4740

gttggtctaa agatgcagaa tatggactct attccatcta tcaaggggga atatttgagc     4800

ttaaggaaaa tgacagaatt tttgtttctg taacaaatga gcacttgata gacatggacc     4860

atgaagccag ttttttcggg gcctttttag ttggctgacc cggggatccg tcgacctgca     4920

gccaagctcc caagcttggc tgttttggcg gatgagagaa gattttcagc ctgatacaga     4980

ttaaatcaga acgcagaagc ggtctgataa aacagaattt gcctggcggc agtagcgcgg     5040

tggtcccacc tgaccccatg ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg     5100

tggggtctcc ccatgcgaga gtagggaact gccaggcatc aaataaaacg aaaggctcag     5160

tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg     5220

acaaatccgc cgggagcgga tttgaacgtt gcgaagcaac ggcccggagg gtggcgggca     5280

ggacgcccgc cataaactgc caggcatcaa attaagcaga aggccatcct gacggatggc     5340

ctttttgcgt ttctacaaac tcttttgttt atttttctaa atacattcaa atatgtatcc     5400

gctcatgaga caataaccct gataaatgct tcaataatgg aagatcttcc aacatcacag     5460

gtaaacagaa acgtcgggtc gatcgggaaa ttctttcccg gacggcgcgg ggttgggcaa     5520

gccgcaggcg cgtcagtgct tttagcgggt gtcggggcgc agccatgacc cagtcacgta     5580

gcgatagcgg agtgtatact ggcttaacta tgcggcatca gagcagattg tactgagagt     5640

gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg     5700

ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt     5760

atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa     5820

gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc     5880

gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag     5940

gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt     6000

gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg     6060

aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg     6120

ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg     6180

taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac     6240

tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg     6300

gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt     6360

taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg     6420

tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc     6480

tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt     6540

ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt     6600

taaatcaatc taaagtatat atgagtaaac ttggtctgac agtctaga                  6648


<210>  8
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- C-Nco I TRAIL 3681 5' primer for construction of 
       pK5079 plasmid

<400>  8
gacgtcccat ggctatgatg gaggtccagg gg                                     32


<210>  9
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- C-Xma I TRAIL 3681 3' primer for construction of 
       pK5079 plasmid

<400>  9
ctgcagcccg ggctagccaa ctaaaaaggc ccc                                    33


<210>  10
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- tar/cheM Primer 1 for construction of pK4946 plasmid

<400>  10
catcgccaat acaccggcct ttataaa                                           27


<210>  11
<211>  75
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- tar/cheM Primer 2 for construction of pK4946 plasmid

<400>  11
ctacacatta tacgagccgg atgattaatt gtcaacagct catttcagaa tcgcgggcga       60

tgaagaggca ctctc                                                        75


<210>  12
<211>  87
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- tar/cheM Primer 3 for construction of pK4946 plasmid

<400>  12
ggctcgtata atgtgtagat gcgtaggcac ctgttacgac gaaccacaca ggaaacagac       60

catgtttaac cgtatccgcg ttgtcac                                           87


<210>  13
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- tar/cheM Primer 4 for construction of pK4946 plasmid

<400>  13
gaagtaggca tccatattgc cattgtc                                           27


<210>  14
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- tsr Primer 1 for construction of pK4947 plasmid

<400>  14
gtcgttattg ataaccgccg gcgtcgc                                           27


<210>  15
<211>  77
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- tsr Primer 2 for construction of pK4947 plasmid

<400>  15
ctacacatta tacgagccgg atgattaatt gtcaacagct catttcagaa tatcacataa       60

aatagcccac gccctcc                                                      77


<210>  16
<211>  88
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- tsr Primer 3 for construction of pK4947 plasmid

<400>  16
ggctcgtata atgtgtagat gcgtaggcac ctgttacgac gaaccacaca ggaaacagac       60

catgttaaag cgaattaaaa ttgttacc                                          88


<210>  17
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- tsr Primer 4 for construction of pK4947 plasmid

<400>  17
ctcgttaatc ttacccgcgc caagaag                                           27


<210>  18
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- D-Trg Up 5' primer for construction of pK4948 plasmid

<400>  18
gacgttattg ttgagcttac tgatggc                                           27


<210>  19
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- D-Trg Up 3' primer for construction of pK4948 plasmid

<400>  19
gctggcgcga cgcttacaca ttttcacaac attcattc                               38


<210>  20
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- D-Trg Down 5' primer for construction of pK4948 
       plasmid

<400>  20
taagcgtcgc gccagcgggc tatttta                                           27


<210>  21
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- D-Trg Down 3' primer for construction of pK4948 
       plasmid

<400>  21
ttagtggtac gccccggtca tcccctg                                           27


<210>  22
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- Tar plus Flag Primer 1 for construction of pK4949 
       plasmid

<400>  22
cgggagagca ggggcgcggt tt                                                22


<210>  23
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- Tar plus Flag Primer 2 for construction of pK4949 
       plasmid

<400>  23
tcgtcatcgt ctttataatc gaaggtttcc cagttcgcat ca                          42


<210>  24
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- Tar plus Flag Primer 3 for construction of pK4949 
       plasmid

<400>  24
gattataaag acgatgacga taagtgatcg acgtgcgctg tcggtta                     47


<210>  25
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- Tsr plus c-Myc Primer 1 for construction of pK4950 
       plasmid

<400>  25
cgcgcgcagg cgagcaggga cgc                                               23


<210>  26
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- Tsr plus c-Myc Primer 2 for construction of pK4950 
       plasmid

<400>  26
cttcggagat cagtttctgt tcaaatgttt cccaattatc gc                          42


<210>  27
<211>  52
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- Tsr plus c-Myc Primer 3 for construction of pK4950 
       plasmid

<400>  27
gaacagaaac tgatctccga agaggatctg taagggcgta gtggtgaaca gt               52


<210>  28
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- Tsr plus c-Myc Primer 4 for construction of pK4950 
       plasmid

<400>  28
gattttgcag agcacgccct gcg                                               23


<210>  29
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- sseL Primer 1 for construction of pK4951 plasmid

<400>  29
cccaagcttg ctgcggcatt gccggtacgt atggattta                              39


<210>  30
<211>  49
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- sseL Primer 2 for construction of pK4951 plasmid

<400>  30
gggataggct ctaagtactc accactcttc tgtatataag ctgtgaaat                   49


<210>  31
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- sseL Primer 3 for construction of pK4951 plasmid

<400>  31
tttcacagct tatatacaga agagtggtga gtacttagag cctatccc                    48


<210>  32
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- sseL Primer 4 for construction of pK4951 plasmid

<400>  32
cgggaattcg gggagaatcg acaactatat ggc                                    33


<210>  33
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- spvD Primer 1 for construction of pK4952 plasmid

<400>  33
cccaagcttc tcagggcaaa tttgccggtg aca                                    33


<210>  34
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- spvD Primer 2 for construction of pK4952 plasmid

<400>  34
taaaatgaat atttaaaaaa gttaagttac actacctcaa taaaatgc                    48


<210>  35
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- spvD Primer 3 for construction of pK4952 plasmid

<400>  35
gcattttatt gaggtagtgt aacttaactt ttttaaatat tcatttta                    48


<210>  36
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- spvD Primer 4 for construction of pK4952 plasmid

<400>  36
cccaagcttg ctgtacacaa aacggactgc acc                                    33


<210>  37
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- ssrAB Primer 1 for construction of pK4953 plasmid

<400>  37
cgggaattcg ctactacttg tggtataata acc                                    33


<210>  38
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- ssrAB Primer 2 for construction of pK4953 plasmid

<400>  38
cttaatacca tcggacgccc ctggaatgct tccctccagt tgcctgtt                    48


<210>  39
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- ssrAB Primer 3 for construction of pK4953 plasmid

<400>  39
aacaggcaac tggagggaag cattccaggg gcgtccgatg gtattaag                    48


<210>  40
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- ssrAB Primer 4 for construction of pK4953 plasmid

<400>  40
cgggaattct gatccgagag attccatccg cta                                    33


<210>  41
<211>  6645
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- plasmid pK4951: suicide vector for construction of 
       sseL deletion mutation

<400>  41
cgcgtttccg agaaccgcgc gaacgacatg gagcggcacg cgggcgtgga aagcctggtc       60

ggctggatcg gcacgatgcg tccggcgtag aggatctgaa gatccagcag ttcaacctgt      120

tgatagtacg tactaagctc tcatgtttca cgtactaagc tctcatgttt aacgtactaa      180

gctctcatgt ttaacgaact aaaccctcat ggctaacgta ctaagctctc atggctaacg      240

tactaagctc tcatgtttca cgtactaagc tctcatgttt gaacaataaa attaatataa      300

atcagcaact taaatagcct ctaaggtttt aagttttata agaaaaaaaa gaatatataa      360

ggcttttaaa gcttttaagg tttaacggtt gtggacaaca agccagggat gtaacgcact      420

gagaagccct tagagcctct caaagcaatt ttcagtgaca caggaacact taacggctga      480

catgggaatt ctgatccttt ttaacccatc acatatacct gccgttcact attatttagt      540

gaaatgagat attatgatat tttctgaatt gtgattaaaa aggcaacttt atgcccatgc      600

aacagaaact ataaaaaata cagagaatga aaagaaacag atagattttt tagttcttta      660

ggcccgtagt ctgcaaatcc ttttatgatt ttctatcaaa caaaagagga aaatagacca      720

gttgcaatcc aaacgagagt ctaatagaat gaggtcgaaa agtaaatcgc gcgggtttgt      780

tactgataaa gcaggcaaga cctaaaatgt gtaaagggca aagtgtatac tttggcgtca      840

ccccttacat attttaggtc tttttttatt gtgcgtaact aacttgccat cttcaaacag      900

gagggctgga agaagcagac cgctaacaca gtacataaaa aaggagacat gaacgatgaa      960

catcaaaaag tttgcaaaac aagcaacagt attaaccttt actaccgcac tgctggcagg     1020

aggcgcaact caagcgtttg cgaaagaaac gaaccaaaag ccatataagg aaacatacgg     1080

catttcccat attacacgcc atgatatgct gcaaatccct gaacagcaaa aaaatgaaaa     1140

atatcaagtt cctgagttcg attcgtccac aattaaaaat atctcttctg caaaaggcct     1200

ggacgtttgg gacagctggc cattacaaaa cgctgacggc actgtcgcaa actatcacgg     1260

ctaccacatc gtctttgcat tagccggaga tcctaaaaat gcggatgaca catcgattta     1320

catgttctat caaaaagtcg gcgaaacttc tattgacagc tggaaaaacg ctggccgcgt     1380

ctttaaagac agcgacaaat tcgatgcaaa tgattctatc ctaaaagacc aaacacaaga     1440

atggtcaggt tcagccacat ttacatctga cggaaaaatc cgtttattct acactgattt     1500

ctccggtaaa cattacggca aacaaacact gacaactgca caagttaacg tatcagcatc     1560

agacagctct ttgaacatca acggtgtaga ggattataaa tcaatctttg acggtgacgg     1620

aaaaacgtat caaaatgtac agcagttcat cgatgaaggc aactacagct caggcgacaa     1680

ccatacgctg agagatcctc actacgtaga agataaaggc cacaaatact tagtatttga     1740

agcaaacact ggaactgaag atggctacca aggcgaagaa tctttattta acaaagcata     1800

ctatggcaaa agcacatcat tcttccgtca agaaagtcaa aaacttctgc aaagcgataa     1860

aaaacgcacg gctgagttag caaacggcgc tctcggtatg attgagctaa acgatgatta     1920

cacactgaaa aaagtgatga aaccgctgat tgcatctaac acagtaacag atgaaattga     1980

acgcgcgaac gtctttaaaa tgaacggcaa atggtatctg ttcactgact cccgcggatc     2040

aaaaatgacg attgacggca ttacgtctaa cgatatttac atgcttggtt atgtttctaa     2100

ttctttaact ggcccataca agccgctgaa caaaactggc cttgtgttaa aaatggatct     2160

tgatcctaac gatgtaacct ttacttactc acacttcgct gtacctcaag cgaaaggaaa     2220

caatgtcgtg attacaagct atatgacaaa cagaggattc tacgcagaca aacaatcaac     2280

gtttgcgccc agcttcctgc tgaacatcaa aggcaagaaa acatctgttg tcaaagacag     2340

catccttgaa caaggacaat taacagttaa caaataaaaa cgcaaaagaa aatgccgata     2400

tcctattggc attttctttt atttcttatc aacataaagg tgaatcccat atgaactata     2460

taaaagcagg caaatggcta accgtattcc taaccttttg gtaatgactc caattattga     2520

tagtgtttta tgttcagata atgcccgatg actttgtcat gcagctccac cgattttgag     2580

aacgacagcg acttccgtcc cagccgtgcc aggtgctgcc tcagattcag gttatgccgc     2640

tcaattcgct gcgtatatcg cttgctgatt acgtgcagct ttcccttcag gcgggattca     2700

tacagcggcc agccatccgt catccatatc accacgtcaa agggtgacag caggctcata     2760

agacgcccca gcgtcgccat agtgcgttca ccgaatacgt gcgcaacaac cgtcttccgg     2820

agactgtcat acgcgtaaaa cagccagcgc tggcgcgatt tagccccgac atagccccac     2880

tgttcgtcca tttccgcgca gacgatgacg tcactgcccg gctgtatgcg cgaggttacc     2940

gactgcggcc tgagtttttt aagtgacgta aaatcgtgtt gaggccaacg cccataatgc     3000

gggctgttgc ccggcatcca acgccattca tggccatatc aatgattttc tggtgcgtac     3060

cgggttgaga agcggtgtaa gtgaactgca tgaattcccg ggagagctcg atatcgcatg     3120

cggtacctct agaagaagct tgctgcggca ttgccggtac gtatggattt aaaaaggaaa     3180

actatccgac atcacagtcc atcggcgcgc cgctgttccg ccagattgaa gagagcggcg     3240

ccgacattgt cgtcaccgat tgtgaaacct gtaagtggca aattgagatg tctacaagca     3300

aacgctgcga acaccccatt acgctactgg cccaggcgct cggctaagta aaaagcgccg     3360

gagcccctcc ggcgccatta ccctactcac cagggacgat tttccatcag acatataccc     3420

ttcatacttc aagttgctta tgtgttggct acggattatt ttgggtataa acgcggatgt     3480

ctctttgcaa taacctgccc tatttggtta accatgaccg ctaacccacg gtggcatgac     3540

agataacgac gttactgttt atagagcaat atctcttgta tcgacgcgtt accagccacc     3600

tttaaagagg gggataactg aatatcccca ataataattg agtgttatgt gaataataag     3660

aaaatcaggt ctatgcctga tttaatatat cccccgctaa taataatatt tttacaaata     3720

attatacatt acatcatatt cgctactttc acttaccagg aaacagagca aaatgaatat     3780

atgtgtaaat tcactttacc gattgagcat accgcaattt cacagcttat atacagaaga     3840

gtggtgagta cttagagcct atcccattag gctcttatcc tgaaatgatg ctctgcgaag     3900

aatttataag tatataaggg tgggaaggcc aggccttccc actaaaagtt ctatgccgtc     3960

aatgattcaa ccacgtctat ccagccatgc tcgctggtaa tgtcctcgcc attaagccaa     4020

cggcgcagca tatttagcgc catcaccgca caaacctcct gacgtattgc cagactatac     4080

cgataggcgc taaaacgcat gcgtaaggca tacgtgccat ccggcatttt tctcaataac     4140

tacgttttgc cgtgcttact gactgaacag atttaaacat cgccaacctg ctgtccagcg     4200

cctcggtgta gagcatcgta tctaccccga ccgcgacaaa attcgcgccc cacgccagac     4260

atttctgcgc cattgccgga tcgaccgcca aaaaacccgc cgcttttccg gcggcgcgaa     4320

tacgataaat acacgcttca atgattcgct gcacttccgg gtgtccggca ttatcggggt     4380

aacccaatga agcagagaga tctgccggac cgataaagac gccatcaatg ccttcaacct     4440

ccagaatcgc gtcaaggttt tccagcgcca ctttgctttc aacctgcacc aacaggcaga     4500

gcgactcatt ggcctgtgcc atatagttgt cgattctccc cgaattccgg atgagcattc     4560

atcaggcggg caagaatgtg aataaaggcc ggataaaact tgtgcttatt tttctttacg     4620

gtctttaaaa aggccgtaat atccagctga acggtctggt tataggtaca ttgagcaact     4680

gactgaaatg cctcaaaatg ttctttacga tgccattggg atatatcaac ggtggtatat     4740

ccagtgattt ttttctccat tttagcttcc ttagctcctg aaaatctcga taactcaaaa     4800

aatacgcccg gtagtgatct tatttcatta tggtgaaagt tggaacctct tacgtgccga     4860

tcaacgtctc attttcgcca aaagttggcc cagggcttcc cggtatcaac agggacacca     4920

ggatttattt attctgcgaa gtgatcttcc gtcacaggta ttagggcccg atcctttttg     4980

tccggtgttg ggttgaaggt gaagccggtc ggggccgcag cgggggccgg cttttcagcc     5040

ttgcccccct gcttcggccg ccgtggctcc ggcgtcttgg gtgccggcgc gggttccgca     5100

gccttggcct gcggtgcggg cacatcggcg ggcttggcct tgatgtgccg cctggcgtgc     5160

gagcggaacg tctcgtagga gaacttgacc ttccccgttt cccgcatgtg ctcccaaatg     5220

gtgacgagcg catagccgga cgctaacgcc gcctcgacat ccgccctcac cgccaggaac     5280

gcaaccgcag cctcatcacg ccggcgcttc ttggccgcgc gggattcaac ccactcggcc     5340

agctcgtcgg tgtagctctt tggcatcgtc tctcgcctgt cccctcagtt cagtaatttc     5400

ctgcatttgc ctgtttccag tcggtagata ttccacaaaa cagcagggaa gcagcgcttt     5460

tccgctgcat aaccctgctt cggggtcatt atagcgattt tttcggtata tccatccttt     5520

ttcgcacgat atacaggatt ttgccaaagg gttcgtgtag actttccttg gtgtatccaa     5580

cggcgtcagc cgggcaggat aggtgaagta ggcccacccg cgagcgggtg ttccttcttc     5640

actgtccctt attcgcacct ggcggtgctc aacgggaatc ctgctctgcg aggctggccg     5700

gctaccgccg gcgtaacaga tgagggcaag cggatggctg atgaaaccaa gccaaccagg     5760

aagggcagcc cacctatcaa ggtgtactgc cttccagacg aacgaagagc gattgaggaa     5820

aaggcggcgg cggccggcat gagcctgtcg gcctacctgc tggccgtcgg ccagggctac     5880

aaaatcacgg gcgtcgtgga ctatgagcac gtccgcgagc tggcccgcat caatggcgac     5940

ctgggccgcc tgggcggcct gctgaaactc tggctcaccg acgacccgcg cacggcgcgg     6000

ttcggtgatg ccacgatcct cgccctgctg gcgaagatcg aagagaagca ggacgagctt     6060

ggcaaggtca tgatgggcgt ggtccgcccg agggcagagc catgactttt ttagccgcta     6120

aaacggccgg ggggtgcgcg tgattgccaa gcacgtcccc atgcgctcca tcaagaagag     6180

cgacttcgcg gagctggtga agtacatcac cgacgagcaa ggcaagaccg agcgcctggg     6240

tcacgtgcgc gtcacgaact gcgaggcaaa caccctgccc gctgtcatgg ccgaggtgat     6300

ggcgacccag cacggcaaca cccgttccga ggccgacaag acctatcacc tgctggttag     6360

cttccgcgcg ggagagaagc ccgacgcgga gacgttgcgc gcgattgagg accgcatctg     6420

cgctgggctt ggcttcgccg agcatcagcg cgtcagtgcc gtgcatcacg acaccgacaa     6480

cctgcacatc catatcgcca tcaacaagat tcacccgacc cgaaacacca tccatgagcc     6540

gtatcgggcc taccgcgccc tcgctgacct ctgcgcgacg ctcgaacggg actacgggct     6600

tgagcgtgac aatcacgaaa cgcggcagcg cgtttccgag aaccg                     6645


<210>  42
<211>  7165
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- plasmid pK4952: suicide vector for construction of 
       spvD deletion mutation

<400>  42
cgcgtttccg agaaccgcgc gaacgacatg gagcggcacg cgggcgtgga aagcctggtc       60

ggctggatcg gcacgatgcg tccggcgtag aggatctgaa gatccagcag ttcaacctgt      120

tgatagtacg tactaagctc tcatgtttca cgtactaagc tctcatgttt aacgtactaa      180

gctctcatgt ttaacgaact aaaccctcat ggctaacgta ctaagctctc atggctaacg      240

tactaagctc tcatgtttca cgtactaagc tctcatgttt gaacaataaa attaatataa      300

atcagcaact taaatagcct ctaaggtttt aagttttata agaaaaaaaa gaatatataa      360

ggcttttaaa gcttttaagg tttaacggtt gtggacaaca agccagggat gtaacgcact      420

gagaagccct tagagcctct caaagcaatt ttcagtgaca caggaacact taacggctga      480

catgggaatt ctgatccttt ttaacccatc acatatacct gccgttcact attatttagt      540

gaaatgagat attatgatat tttctgaatt gtgattaaaa aggcaacttt atgcccatgc      600

aacagaaact ataaaaaata cagagaatga aaagaaacag atagattttt tagttcttta      660

ggcccgtagt ctgcaaatcc ttttatgatt ttctatcaaa caaaagagga aaatagacca      720

gttgcaatcc aaacgagagt ctaatagaat gaggtcgaaa agtaaatcgc gcgggtttgt      780

tactgataaa gcaggcaaga cctaaaatgt gtaaagggca aagtgtatac tttggcgtca      840

ccccttacat attttaggtc tttttttatt gtgcgtaact aacttgccat cttcaaacag      900

gagggctgga agaagcagac cgctaacaca gtacataaaa aaggagacat gaacgatgaa      960

catcaaaaag tttgcaaaac aagcaacagt attaaccttt actaccgcac tgctggcagg     1020

aggcgcaact caagcgtttg cgaaagaaac gaaccaaaag ccatataagg aaacatacgg     1080

catttcccat attacacgcc atgatatgct gcaaatccct gaacagcaaa aaaatgaaaa     1140

atatcaagtt cctgagttcg attcgtccac aattaaaaat atctcttctg caaaaggcct     1200

ggacgtttgg gacagctggc cattacaaaa cgctgacggc actgtcgcaa actatcacgg     1260

ctaccacatc gtctttgcat tagccggaga tcctaaaaat gcggatgaca catcgattta     1320

catgttctat caaaaagtcg gcgaaacttc tattgacagc tggaaaaacg ctggccgcgt     1380

ctttaaagac agcgacaaat tcgatgcaaa tgattctatc ctaaaagacc aaacacaaga     1440

atggtcaggt tcagccacat ttacatctga cggaaaaatc cgtttattct acactgattt     1500

ctccggtaaa cattacggca aacaaacact gacaactgca caagttaacg tatcagcatc     1560

agacagctct ttgaacatca acggtgtaga ggattataaa tcaatctttg acggtgacgg     1620

aaaaacgtat caaaatgtac agcagttcat cgatgaaggc aactacagct caggcgacaa     1680

ccatacgctg agagatcctc actacgtaga agataaaggc cacaaatact tagtatttga     1740

agcaaacact ggaactgaag atggctacca aggcgaagaa tctttattta acaaagcata     1800

ctatggcaaa agcacatcat tcttccgtca agaaagtcaa aaacttctgc aaagcgataa     1860

aaaacgcacg gctgagttag caaacggcgc tctcggtatg attgagctaa acgatgatta     1920

cacactgaaa aaagtgatga aaccgctgat tgcatctaac acagtaacag atgaaattga     1980

acgcgcgaac gtctttaaaa tgaacggcaa atggtatctg ttcactgact cccgcggatc     2040

aaaaatgacg attgacggca ttacgtctaa cgatatttac atgcttggtt atgtttctaa     2100

ttctttaact ggcccataca agccgctgaa caaaactggc cttgtgttaa aaatggatct     2160

tgatcctaac gatgtaacct ttacttactc acacttcgct gtacctcaag cgaaaggaaa     2220

caatgtcgtg attacaagct atatgacaaa cagaggattc tacgcagaca aacaatcaac     2280

gtttgcgccc agcttcctgc tgaacatcaa aggcaagaaa acatctgttg tcaaagacag     2340

catccttgaa caaggacaat taacagttaa caaataaaaa cgcaaaagaa aatgccgata     2400

tcctattggc attttctttt atttcttatc aacataaagg tgaatcccat atgaactata     2460

taaaagcagg caaatggcta accgtattcc taaccttttg gtaatgactc caattattga     2520

tagtgtttta tgttcagata atgcccgatg actttgtcat gcagctccac cgattttgag     2580

aacgacagcg acttccgtcc cagccgtgcc aggtgctgcc tcagattcag gttatgccgc     2640

tcaattcgct gcgtatatcg cttgctgatt acgtgcagct ttcccttcag gcgggattca     2700

tacagcggcc agccatccgt catccatatc accacgtcaa agggtgacag caggctcata     2760

agacgcccca gcgtcgccat agtgcgttca ccgaatacgt gcgcaacaac cgtcttccgg     2820

agactgtcat acgcgtaaaa cagccagcgc tggcgcgatt tagccccgac atagccccac     2880

tgttcgtcca tttccgcgca gacgatgacg tcactgcccg gctgtatgcg cgaggttacc     2940

gactgcggcc tgagtttttt aagtgacgta aaatcgtgtt gaggccaacg cccataatgc     3000

gggctgttgc ccggcatcca acgccattca tggccatatc aatgattttc tggtgcgtac     3060

cgggttgaga agcggtgtaa gtgaactgca tgaattcccg ggagagctcg atatcgcatg     3120

cggtacctct agaagaagct tctcagggca aatttgccgg tgacaagttc cacatcagtg     3180

tgctcaggga tatggtgcca caagcatttc aagcgctgtc cggattgctg ttttcagagg     3240

acagtccggt agataagtgg aaagtgaccg atatggagaa ggtcgttcaa caagcccgtg     3300

ttagcctggg cgctcagttc acgttgtata taaaaccaga ccaggaaaat tcgcagtaca     3360

gtgcgtcgtt tctccacaag acacggcaat ttatagagtg tctggaatcc agactatccg     3420

aaaatggggt tatttcagga cagtgtcctg agtcagacgt tcatcctgaa aattggaaat     3480

atctcagtta tcgtaatgaa ctacgaagtg ggcgtgatgg tggcgaaatg cagagacagg     3540

ctttacgtga ggaaccgttt tatcgtttga tgacagagta agtatgggtt tggggagcaa     3600

cggaacagta aacgccgtta aacagctatt ttaaatgctc attaatttat taatcaataa     3660

attacaaatt ttcattgaag gctcccccct tactgacgaa ttccggcacc gtaaaggaat     3720

aacgctcatg catattgata tgtccgcact gtaatggtga aaattacata agcaagagcg     3780

ttttttgaaa aatattatat ttaatgtttt gtaatatgca ttttattgag gtagtgtaac     3840

ttaacttttt taaatattca ttttatgaat ggtaagttaa tagagagcct tagcaagatc     3900

cgctgtaaac cctcgcccaa tgcgggcggg gatataaggc gaaaagcccg aagggcttta     3960

gggcaggcta ctccggcgta ttctgtctga gcacgtatgc tttaagcgtt tctatcgtgg     4020

caccaccggc gctacaggca aagtaagaac gagaccacag cagcccggtt ttgctctgca     4080

tccgcaagtg ggtattctgc tgacggagaa gccgcgacga taccgacttt aaattattta     4140

ccatcacgct gacccccagt tttagcggat acgctatcag cagatggacg tgatcttgtt     4200

caccatccat ctcgataatt tcgcactcca actttgccgc agccgaacca aaagcatcac     4260

gtaactgagc gataatctgc ccgtcaaaca gcttgcagcg gtattttgtc gtaaagatta     4320

atgcacaacc agcttactta cactatgccg tttacgaaga aagccttcca gtgattcgtg     4380

gtgattactc aattgaattt ttcacttaac atgttaaaat aaatacaata tattaatgag     4440

cgctgaatat gttaagagcc acgaaagtat gcatatatcc gacaccggaa caggcggagc     4500

accttaacgc ccagttcggt gcagtccgtt ttgtgtacag caagcttggg atcgggccct     4560

atcacttatt caggcgtagc aaccaggcgt ttaagggcac caataactgc cttaaaaaaa     4620

ttacgccccg ccctgccact catcgcagta ctgttgtaat tcattaagca ttctgccgac     4680

atggaagcca tcacagacgg catgatgaac ctgaatcgcc agcggcatca gcaccttgtc     4740

gccttgcgta taatatttgc ccatggtgaa aacgggggcg aagaagttgt ccatattggc     4800

cacgtttaaa tcaaaactgg tgaaactcac ccagggattg gctgagacga aaaacatatt     4860

ctcaataaac cctttaggga aataggccag gttttcaccg taacacgcca catcttgcga     4920

atatatgtgt agaaactgcc ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt     4980

ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca ctatcccata tcaccagctc     5040

accgtctttc attgccatac ggaattccgg atgagcattc atcaggcggg caagaatgtg     5100

aataaaggcc ggataaaact tgtgcttatt tttctttacg gtctttaaaa aggccgtaat     5160

atccagctga acggtctggt tataggtaca ttgagcaact gactgaaatg cctcaaaatg     5220

ttctttacga tgccattggg atatatcaac ggtggtatat ccagtgattt ttttctccat     5280

tttagcttcc ttagctcctg aaaatctcga taactcaaaa aatacgcccg gtagtgatct     5340

tatttcatta tggtgaaagt tggaacctct tacgtgccga tcaacgtctc attttcgcca     5400

aaagttggcc cagggcttcc cggtatcaac agggacacca ggatttattt attctgcgaa     5460

gtgatcttcc gtcacaggta ttagggcccg atcctttttg tccggtgttg ggttgaaggt     5520

gaagccggtc ggggccgcag cgggggccgg cttttcagcc ttgcccccct gcttcggccg     5580

ccgtggctcc ggcgtcttgg gtgccggcgc gggttccgca gccttggcct gcggtgcggg     5640

cacatcggcg ggcttggcct tgatgtgccg cctggcgtgc gagcggaacg tctcgtagga     5700

gaacttgacc ttccccgttt cccgcatgtg ctcccaaatg gtgacgagcg catagccgga     5760

cgctaacgcc gcctcgacat ccgccctcac cgccaggaac gcaaccgcag cctcatcacg     5820

ccggcgcttc ttggccgcgc gggattcaac ccactcggcc agctcgtcgg tgtagctctt     5880

tggcatcgtc tctcgcctgt cccctcagtt cagtaatttc ctgcatttgc ctgtttccag     5940

tcggtagata ttccacaaaa cagcagggaa gcagcgcttt tccgctgcat aaccctgctt     6000

cggggtcatt atagcgattt tttcggtata tccatccttt ttcgcacgat atacaggatt     6060

ttgccaaagg gttcgtgtag actttccttg gtgtatccaa cggcgtcagc cgggcaggat     6120

aggtgaagta ggcccacccg cgagcgggtg ttccttcttc actgtccctt attcgcacct     6180

ggcggtgctc aacgggaatc ctgctctgcg aggctggccg gctaccgccg gcgtaacaga     6240

tgagggcaag cggatggctg atgaaaccaa gccaaccagg aagggcagcc cacctatcaa     6300

ggtgtactgc cttccagacg aacgaagagc gattgaggaa aaggcggcgg cggccggcat     6360

gagcctgtcg gcctacctgc tggccgtcgg ccagggctac aaaatcacgg gcgtcgtgga     6420

ctatgagcac gtccgcgagc tggcccgcat caatggcgac ctgggccgcc tgggcggcct     6480

gctgaaactc tggctcaccg acgacccgcg cacggcgcgg ttcggtgatg ccacgatcct     6540

cgccctgctg gcgaagatcg aagagaagca ggacgagctt ggcaaggtca tgatgggcgt     6600

ggtccgcccg agggcagagc catgactttt ttagccgcta aaacggccgg ggggtgcgcg     6660

tgattgccaa gcacgtcccc atgcgctcca tcaagaagag cgacttcgcg gagctggtga     6720

agtacatcac cgacgagcaa ggcaagaccg agcgcctggg tcacgtgcgc gtcacgaact     6780

gcgaggcaaa caccctgccc gctgtcatgg ccgaggtgat ggcgacccag cacggcaaca     6840

cccgttccga ggccgacaag acctatcacc tgctggttag cttccgcgcg ggagagaagc     6900

ccgacgcgga gacgttgcgc gcgattgagg accgcatctg cgctgggctt ggcttcgccg     6960

agcatcagcg cgtcagtgcc gtgcatcacg acaccgacaa cctgcacatc catatcgcca     7020

tcaacaagat tcacccgacc cgaaacacca tccatgagcc gtatcgggcc taccgcgccc     7080

tcgctgacct ctgcgcgacg ctcgaacggg actacgggct tgagcgtgac aatcacgaaa     7140

cgcggcagcg cgtttccgag aaccg                                           7165


<210>  43
<211>  7165
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- plasmid pK4953: suicide vector for construction of 
       ssrAB deletion mutation

<400>  43
cgcgtttccg agaaccgcgc gaacgacatg gagcggcacg cgggcgtgga aagcctggtc       60

ggctggatcg gcacgatgcg tccggcgtag aggatctgaa gatccagcag ttcaacctgt      120

tgatagtacg tactaagctc tcatgtttca cgtactaagc tctcatgttt aacgtactaa      180

gctctcatgt ttaacgaact aaaccctcat ggctaacgta ctaagctctc atggctaacg      240

tactaagctc tcatgtttca cgtactaagc tctcatgttt gaacaataaa attaatataa      300

atcagcaact taaatagcct ctaaggtttt aagttttata agaaaaaaaa gaatatataa      360

ggcttttaaa gcttttaagg tttaacggtt gtggacaaca agccagggat gtaacgcact      420

gagaagccct tagagcctct caaagcaatt ttcagtgaca caggaacact taacggctga      480

catgggaatt ctgatccttt ttaacccatc acatatacct gccgttcact attatttagt      540

gaaatgagat attatgatat tttctgaatt gtgattaaaa aggcaacttt atgcccatgc      600

aacagaaact ataaaaaata cagagaatga aaagaaacag atagattttt tagttcttta      660

ggcccgtagt ctgcaaatcc ttttatgatt ttctatcaaa caaaagagga aaatagacca      720

gttgcaatcc aaacgagagt ctaatagaat gaggtcgaaa agtaaatcgc gcgggtttgt      780

tactgataaa gcaggcaaga cctaaaatgt gtaaagggca aagtgtatac tttggcgtca      840

ccccttacat attttaggtc tttttttatt gtgcgtaact aacttgccat cttcaaacag      900

gagggctgga agaagcagac cgctaacaca gtacataaaa aaggagacat gaacgatgaa      960

catcaaaaag tttgcaaaac aagcaacagt attaaccttt actaccgcac tgctggcagg     1020

aggcgcaact caagcgtttg cgaaagaaac gaaccaaaag ccatataagg aaacatacgg     1080

catttcccat attacacgcc atgatatgct gcaaatccct gaacagcaaa aaaatgaaaa     1140

atatcaagtt cctgagttcg attcgtccac aattaaaaat atctcttctg caaaaggcct     1200

ggacgtttgg gacagctggc cattacaaaa cgctgacggc actgtcgcaa actatcacgg     1260

ctaccacatc gtctttgcat tagccggaga tcctaaaaat gcggatgaca catcgattta     1320

catgttctat caaaaagtcg gcgaaacttc tattgacagc tggaaaaacg ctggccgcgt     1380

ctttaaagac agcgacaaat tcgatgcaaa tgattctatc ctaaaagacc aaacacaaga     1440

atggtcaggt tcagccacat ttacatctga cggaaaaatc cgtttattct acactgattt     1500

ctccggtaaa cattacggca aacaaacact gacaactgca caagttaacg tatcagcatc     1560

agacagctct ttgaacatca acggtgtaga ggattataaa tcaatctttg acggtgacgg     1620

aaaaacgtat caaaatgtac agcagttcat cgatgaaggc aactacagct caggcgacaa     1680

ccatacgctg agagatcctc actacgtaga agataaaggc cacaaatact tagtatttga     1740

agcaaacact ggaactgaag atggctacca aggcgaagaa tctttattta acaaagcata     1800

ctatggcaaa agcacatcat tcttccgtca agaaagtcaa aaacttctgc aaagcgataa     1860

aaaacgcacg gctgagttag caaacggcgc tctcggtatg attgagctaa acgatgatta     1920

cacactgaaa aaagtgatga aaccgctgat tgcatctaac acagtaacag atgaaattga     1980

acgcgcgaac gtctttaaaa tgaacggcaa atggtatctg ttcactgact cccgcggatc     2040

aaaaatgacg attgacggca ttacgtctaa cgatatttac atgcttggtt atgtttctaa     2100

ttctttaact ggcccataca agccgctgaa caaaactggc cttgtgttaa aaatggatct     2160

tgatcctaac gatgtaacct ttacttactc acacttcgct gtacctcaag cgaaaggaaa     2220

caatgtcgtg attacaagct atatgacaaa cagaggattc tacgcagaca aacaatcaac     2280

gtttgcgccc agcttcctgc tgaacatcaa aggcaagaaa acatctgttg tcaaagacag     2340

catccttgaa caaggacaat taacagttaa caaataaaaa cgcaaaagaa aatgccgata     2400

tcctattggc attttctttt atttcttatc aacataaagg tgaatcccat atgaactata     2460

taaaagcagg caaatggcta accgtattcc taaccttttg gtaatgactc caattattga     2520

tagtgtttta tgttcagata atgcccgatg actttgtcat gcagctccac cgattttgag     2580

aacgacagcg acttccgtcc cagccgtgcc aggtgctgcc tcagattcag gttatgccgc     2640

tcaattcgct gcgtatatcg cttgctgatt acgtgcagct ttcccttcag gcgggattca     2700

tacagcggcc agccatccgt catccatatc accacgtcaa agggtgacag caggctcata     2760

agacgcccca gcgtcgccat agtgcgttca ccgaatacgt gcgcaacaac cgtcttccgg     2820

agactgtcat acgcgtaaaa cagccagcgc tggcgcgatt tagccccgac atagccccac     2880

tgttcgtcca tttccgcgca gacgatgacg tcactgcccg gctgtatgcg cgaggttacc     2940

gactgcggcc tgagtttttt aagtgacgta aaatcgtgtt gaggccaacg cccataatgc     3000

gggctgttgc ccggcatcca acgccattca tggccatatc aatgattttc tggtgcgtac     3060

cgggttgaga agcggtgtaa gtgaactgca tgaattcccg ggagagctcg atatcgcatg     3120

cggtacctct agaagaagct tgggatcggg ccctatcact tattcaggcg tagcaaccag     3180

gcgtttaagg gcaccaataa ctgccttaaa aaaattacgc cccgccctgc cactcatcgc     3240

agtactgttg taattcatta agcattctgc cgacatggaa gccatcacag acggcatgat     3300

gaacctgaat cgccagcggc atcagcacct tgtcgccttg cgtataatat ttgcccatgg     3360

tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac     3420

tcacccaggg attggctgag acgaaaaaca tattctcaat aaacccttta gggaaatagg     3480

ccaggttttc accgtaacac gccacatctt gcgaatatat gtgtagaaac tgccggaaat     3540

cgtcgtggta ttcactccag agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt     3600

aacaagggtg aacactatcc catatcacca gctcaccgtc tttcattgcc atacggaatt     3660

cgctactact tgtggtataa taaccgttta accatccccc atccgctgtg agctgtatag     3720

cataatcatg gacgtccggg tgtgctgcaa gcagtagtgt cacataggca agacaaggct     3780

taggtaagct ttccaggtca tttaagaaca aagaaataga aaatgcttct gagaaaattt     3840

ctcctctggc aggatgccca tcaatagtca ttatccagga tcggctatta ccttcggccc     3900

tgatatcctg aattaatgga atgcctttta aaactgccag catgaatccc tcctcagaca     3960

taaatgggag tttctatcaa attcgctcac aaccacatcc gtaaaaagcc tgattcacat     4020

ttatttcgac tatactcttc ttgtacaata tcaggatgct gtctacatat accttgtcac     4080

aggcgattct atcattcgga ttttccgata aattcacaat tacattttca gcattgacat     4140

aaaaacttac aatttgaaaa attatttatt aaataaactg ttacgatgtt tttacatcgc     4200

catcttatta aaaagtaatt gtagtcatcg actgggttat atatgaagaa atttatcttc     4260

ctaatgataa caccatcgat taatcttctg atgaaactat atgtactgcg atagtgatca     4320

agtgccaaag attttgcaac aggcaactgg agggaagcat tccaggggcg tccgatggta     4380

ttaagcattg gtcatatttt gatgagcctt acgccacgca gtattgctca tcatcgacaa     4440

aatccatacg gatgccctgg tatgccgcac catttatcac taccttagtc ttcatttgat     4500

catgatatag tagaatcccc ttatttaacg ggctttacca tgtcgtattc tatcggcgaa     4560

tttgccagac tatgcggtat caatgccgcc acgctaaggg catggcagcg acgctatggt     4620

ttattgaaac cgcagcgtac tgatggcgga catcgcttat acagcgatga cgatattcga     4680

caagcgctta gcattctcga ctgggtgaga aaaggcgtac cgataagcca ggtcaaaccc     4740

ttactgtcgc gtccggttat tcgcctgggc gataactgga taacgatcca ggagacgatg     4800

cttcaacatc tccacgaagg gcgaattgac gcgctgcggc agttgattta tgactgtggc     4860

cgggaatatc cccgcgcaga actggtgacc catttattgc gtccgttgcg cagcaaagtg     4920

tccgcgcatc ttcccgccgt gatgacgctg cgcgaaatac tggatggcat cattattgcc     4980

tacacctctt tttgccttga aggcgacaga aaagcgcctg gcaacaatgc ctttattagc     5040

ggatggaatc tctcggatca cgaattccgg atgagcattc atcaggcggg caagaatgtg     5100

aataaaggcc ggataaaact tgtgcttatt tttctttacg gtctttaaaa aggccgtaat     5160

atccagctga acggtctggt tataggtaca ttgagcaact gactgaaatg cctcaaaatg     5220

ttctttacga tgccattggg atatatcaac ggtggtatat ccagtgattt ttttctccat     5280

tttagcttcc ttagctcctg aaaatctcga taactcaaaa aatacgcccg gtagtgatct     5340

tatttcatta tggtgaaagt tggaacctct tacgtgccga tcaacgtctc attttcgcca     5400

aaagttggcc cagggcttcc cggtatcaac agggacacca ggatttattt attctgcgaa     5460

gtgatcttcc gtcacaggta ttagggcccg atcctttttg tccggtgttg ggttgaaggt     5520

gaagccggtc ggggccgcag cgggggccgg cttttcagcc ttgcccccct gcttcggccg     5580

ccgtggctcc ggcgtcttgg gtgccggcgc gggttccgca gccttggcct gcggtgcggg     5640

cacatcggcg ggcttggcct tgatgtgccg cctggcgtgc gagcggaacg tctcgtagga     5700

gaacttgacc ttccccgttt cccgcatgtg ctcccaaatg gtgacgagcg catagccgga     5760

cgctaacgcc gcctcgacat ccgccctcac cgccaggaac gcaaccgcag cctcatcacg     5820

ccggcgcttc ttggccgcgc gggattcaac ccactcggcc agctcgtcgg tgtagctctt     5880

tggcatcgtc tctcgcctgt cccctcagtt cagtaatttc ctgcatttgc ctgtttccag     5940

tcggtagata ttccacaaaa cagcagggaa gcagcgcttt tccgctgcat aaccctgctt     6000

cggggtcatt atagcgattt tttcggtata tccatccttt ttcgcacgat atacaggatt     6060

ttgccaaagg gttcgtgtag actttccttg gtgtatccaa cggcgtcagc cgggcaggat     6120

aggtgaagta ggcccacccg cgagcgggtg ttccttcttc actgtccctt attcgcacct     6180

ggcggtgctc aacgggaatc ctgctctgcg aggctggccg gctaccgccg gcgtaacaga     6240

tgagggcaag cggatggctg atgaaaccaa gccaaccagg aagggcagcc cacctatcaa     6300

ggtgtactgc cttccagacg aacgaagagc gattgaggaa aaggcggcgg cggccggcat     6360

gagcctgtcg gcctacctgc tggccgtcgg ccagggctac aaaatcacgg gcgtcgtgga     6420

ctatgagcac gtccgcgagc tggcccgcat caatggcgac ctgggccgcc tgggcggcct     6480

gctgaaactc tggctcaccg acgacccgcg cacggcgcgg ttcggtgatg ccacgatcct     6540

cgccctgctg gcgaagatcg aagagaagca ggacgagctt ggcaaggtca tgatgggcgt     6600

ggtccgcccg agggcagagc catgactttt ttagccgcta aaacggccgg ggggtgcgcg     6660

tgattgccaa gcacgtcccc atgcgctcca tcaagaagag cgacttcgcg gagctggtga     6720

agtacatcac cgacgagcaa ggcaagaccg agcgcctggg tcacgtgcgc gtcacgaact     6780

gcgaggcaaa caccctgccc gctgtcatgg ccgaggtgat ggcgacccag cacggcaaca     6840

cccgttccga ggccgacaag acctatcacc tgctggttag cttccgcgcg ggagagaagc     6900

ccgacgcgga gacgttgcgc gcgattgagg accgcatctg cgctgggctt ggcttcgccg     6960

agcatcagcg cgtcagtgcc gtgcatcacg acaccgacaa cctgcacatc catatcgcca     7020

tcaacaagat tcacccgacc cgaaacacca tccatgagcc gtatcgggcc taccgcgccc     7080

tcgctgacct ctgcgcgacg ctcgaacggg actacgggct tgagcgtgac aatcacgaaa     7140

cgcggcagcg cgtttccgag aaccg                                           7165


<210>  44
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- Tar plus Flag Primer 4 for construction of pK4949 
       plasmid

<400>  44
ccgtggcggc gacgcgcatg ttc                                               23


