                               SEQUENCE LISTING

<110> UNIVERSITY OF GEORGIA RESEARCH FOUNDATION
 
<120> BROADLY REACTIVE IMMUNOGENS OF H5 INFLUENZA VIRUS, COMPOSITIONS 
      AND METHODS OF USE THEREOF

<130> 173093.011001/PCT

<140> PCT/US2020/018970
<141> 2020-02-20

<150> 62/808,341
<151> 2019-02-21

<160> 5     

<170> PatentIn version 3.5

<210> 1
<211> 575
<212> PRT
<213> Influenza A virus


<220>
<221> MOD_RES
<222> (77)..(77)
<223> Any amino acid

<400> 1
Met Glu Lys Ile Val Leu Leu Leu Ala Thr Val Ser Leu Val Lys Ser 
1               5                   10                  15      


Asp Gln Ile Cys Ile Gly Tyr His Ala Asn Asn Ser Thr Glu Gln Val 
            20                  25                  30          


Asp Thr Ile Met Glu Lys Asn Val Thr Val Thr His Ala Gln Asp Ile 
        35                  40                  45              


Leu Glu Arg Thr His Asn Gly Lys Leu Cys Asp Leu Asn Gly Val Lys 
    50                  55                  60                  


Pro Leu Ile Leu Arg Asp Cys Ser Val Ala Gly Trp Xaa Leu Gly Asn 
65                  70                  75                  80  


Pro Met Cys Asp Glu Phe Leu Asn Val Pro Glu Trp Ser Tyr Ile Val 
                85                  90                  95      


Glu Lys Thr Ser Pro Ala Asn Asp Leu Cys Tyr Pro Gly His Phe Asn 
            100                 105                 110         


Asp Tyr Glu Glu Leu Lys His Leu Leu Ser Arg Ile Asn His Phe Glu 
        115                 120                 125             


Lys Ile Gln Ile Ile Pro Lys Ser Ser Trp Ser Asn His Asp Ala Ser 
    130                 135                 140                 


Ser Gly Val Ser Ser Ala Cys Pro Tyr Leu Gly Arg Ser Ser Phe Phe 
145                 150                 155                 160 


Arg Asn Val Val Trp Leu Ile Lys Lys Asn Ser Ala Tyr Pro Thr Ile 
                165                 170                 175     


Lys Arg Ser Tyr Asn Asn Thr Asn Gln Glu Asp Leu Leu Val Leu Trp 
            180                 185                 190         


Gly Ile His His Pro Asn Asp Ala Ala Glu Gln Ile Lys Leu Tyr Gln 
        195                 200                 205             


Asn Pro Thr Ser Tyr Ile Ser Val Gly Thr Ser Thr Leu Asn Gln Arg 
    210                 215                 220                 


Leu Val Pro Glu Ile Ala Thr Arg Pro Lys Val Asn Gly Gln Ser Gly 
225                 230                 235                 240 


Arg Met Glu Phe Phe Trp Thr Ile Leu Lys Pro Asn Asp Ala Ile Asn 
                245                 250                 255     


Phe Glu Ser Asn Gly Asn Phe Ile Ala Pro Glu Tyr Ala Tyr Lys Ile 
            260                 265                 270         


Val Lys Lys Gly Asp Ser Thr Ile Met Lys Ser Glu Leu Glu Tyr Gly 
        275                 280                 285             


Asn Cys Asn Thr Lys Cys Gln Thr Pro Met Gly Ala Ile Asn Ser Ser 
    290                 295                 300                 


Met Pro Phe His Asn Ile His Pro Leu Thr Ile Gly Glu Cys Pro Lys 
305                 310                 315                 320 


Tyr Val Lys Ser Asn Arg Leu Val Leu Ala Thr Gly Leu Arg Asn Thr 
                325                 330                 335     


Pro Gln Arg Glu Arg Arg Arg Lys Lys Arg Gly Leu Phe Gly Ala Ile 
            340                 345                 350         


Ala Gly Phe Ile Glu Gly Gly Trp Gln Gly Met Val Asp Gly Trp Tyr 
        355                 360                 365             


Gly Tyr His His Ser Asn Glu Gln Gly Ser Gly Tyr Ala Ala Asp Lys 
    370                 375                 380                 


Glu Ser Thr Gln Lys Ala Ile Asp Gly Val Thr Asn Lys Val Asn Ser 
385                 390                 395                 400 


Ile Ile Asn Lys Met Asn Thr Gln Phe Glu Ala Val Gly Arg Glu Phe 
                405                 410                 415     


Asn Asn Leu Glu Arg Arg Ile Glu Asn Leu Asn Lys Lys Met Glu Asp 
            420                 425                 430         


Gly Phe Leu Asp Val Trp Thr Tyr Asn Ala Glu Leu Leu Val Leu Met 
        435                 440                 445             


Glu Asn Glu Arg Thr Leu Asp Phe His Asp Ser Asn Val Lys Asn Leu 
    450                 455                 460                 


Tyr Asp Lys Val Arg Leu Gln Leu Arg Asp Asn Ala Lys Glu Leu Gly 
465                 470                 475                 480 


Asn Gly Cys Phe Glu Phe Tyr His Lys Cys Asp Asn Glu Cys Met Glu 
                485                 490                 495     


Ser Val Lys Asn Gly Thr Tyr Asp Tyr Pro Gln Tyr Ser Glu Glu Ala 
            500                 505                 510         


Arg Leu Asn Arg Glu Glu Ile Ser Gly Val Lys Leu Glu Ser Met Gly 
        515                 520                 525             


Thr Tyr Gln Ile Leu Ser Ile Tyr Ser Thr Val Ala Ser Ser Leu Ala 
    530                 535                 540                 


Leu Ala Ile Met Val Ala Gly Leu Ser Leu Trp Met Cys Ser Asn Gly 
545                 550                 555                 560 


Ser Leu Gln Cys Arg Ile Cys Ile Tyr Phe Cys Glu Phe Arg Leu 
                565                 570                 575 


<210> 2
<211> 1741
<212> DNA
<213> Influenza A virus

<400> 2
ctgtcaaaat ggagaaaata gtgcttcttc ttgcaacagt cagtcttgtt aaaagtgatc       60

agatttgcat tggttaccat gcaaacaact cgacagagca ggttgacaca ataatggaaa      120

agaatgttac tgttacacat gcccaagaca tactggaaag gacacacaac gggaagctct      180

gcgatctaaa tggagtgaag cctctcattt tgagggattg tagtgtagct ggatggstcc      240

tcggaaaccc tatgtgtgac gaattcctca atgtgccgga atggtcttac atagtagaga      300

agaccagtcc agccaatgac ctctgttatc cagggcattt caacgactat gaagaactga      360

aacacctatt gagcagaata aaccattttg agaaaattca gatcatcccc aaaagttctt      420

ggtccaatca tgatgcctca tcaggggtga gctcagcatg tccatacctt gggaggtcct      480

cctttttcag aaatgtggta tggcttatca aaaagaacag tgcataccca acaataaaga      540

ggagctacaa taataccaac caagaagatc ttttggtact gtgggggatt caccatccta      600

atgatgcggc agagcagata aagctctatc aaaatccaac ctcctacatt tccgttggaa      660

catcaacact gaaccagaga ttggttccag aaatagctac tagacccaaa gtaaacgggc      720

aaagtggaag aatggagttc ttctggacaa ttttaaagcc gaatgatgcc atcaatttcg      780

agagtaatgg aaatttcatt gctccagaat atgcatacaa aattgtcaag aaaggggact      840

caacaattat gaaaagtgaa ttggaatatg gtaactgcaa caccaagtgt caaactccaa      900

tgggggcgat aaactctagt atgccattcc acaacataca ccccctcacc atcggggaat      960

gccccaaata tgtgaaatca aacagattag tccttgcgac tggactcaga aatacccctc     1020

aaagagagag aaggagaaaa aagagaggac tatttggagc tatagcaggt tttatagagg     1080

gaggatggca gggaatggta gatggttggt atgggtatca ccatagcaat gagcagggga     1140

gtggatacgc tgcagacaaa gaatccactc aaaaggcaat agatggagtc accaataagg     1200

tcaactcgat cattaacaaa atgaacactc agtttgaggc cgttggaagg gaatttaata     1260

acttggaaag gaggatagag aatttaaaca agaagatgga agacggattc ctagatgtct     1320

ggacctacaa tgctgaactt ctggttctca tggaaaatga gagaactctc gactttcatg     1380

actcaaacgt caagaacctt tacgacaagg tccgactaca gcttagggat aatgcaaagg     1440

agctgggtaa tggttgtttc gaattctatc acaaatgtga taatgaatgt atggaaagtg     1500

tgaaaaacgg aacgtatgac tacccgcagt attcagaaga agcaagacta aacagagagg     1560

aaataagtgg agtaaaattg gagtcaatgg gaacttacca aatactgtca atttattcaa     1620

cagtggcgag ttccctagca ctggcaatca tggtagctgg tctatcttta tggatgtgct     1680

ccaatggatc gttacaatgc agaatttgca tttatttttg tgagttcaga ttgtagttaa     1740

a                                                                     1741


<210> 3
<211> 568
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 3
Met Glu Lys Ile Val Leu Leu Phe Ala Ile Val Ser Leu Val Lys Ser 
1               5                   10                  15      


Asp Gln Ile Cys Ile Gly Tyr His Ala Asn Asn Ser Thr Glu Gln Val 
            20                  25                  30          


Asp Thr Ile Met Glu Lys Asn Val Thr Val Thr His Ala Gln Asp Ile 
        35                  40                  45              


Leu Glu Lys Thr His Asn Gly Lys Leu Cys Asp Leu Asn Gly Val Lys 
    50                  55                  60                  


Pro Leu Ile Leu Lys Asp Cys Ser Val Ala Gly Trp Leu Leu Gly Asn 
65                  70                  75                  80  


Pro Met Cys Asp Glu Phe Ile Asn Val Pro Glu Trp Ser Tyr Ile Val 
                85                  90                  95      


Glu Lys Ala Asn Pro Ala Asn Asp Leu Cys Tyr Pro Gly Asn Phe Asn 
            100                 105                 110         


Asp Tyr Glu Glu Leu Lys His Leu Leu Ser Arg Ile Asn His Phe Glu 
        115                 120                 125             


Lys Ile Gln Ile Ile Pro Lys Asp Ser Trp Ser Asp His Glu Ala Ser 
    130                 135                 140                 


Leu Gly Val Ser Ala Ala Cys Pro Tyr Gln Gly Asn Ser Ser Phe Phe 
145                 150                 155                 160 


Arg Asn Val Val Trp Leu Ile Lys Lys Asn Asp Ala Tyr Pro Thr Ile 
                165                 170                 175     


Lys Lys Ser Tyr Asn Asn Thr Asn Arg Glu Asp Leu Leu Val Leu Trp 
            180                 185                 190         


Gly Ile His His Pro Asn Asp Ala Ala Glu Gln Thr Arg Leu Tyr Gln 
        195                 200                 205             


Asn Pro Thr Thr Tyr Ile Ser Val Gly Thr Ser Thr Leu Asn Gln Arg 
    210                 215                 220                 


Leu Val Pro Lys Ile Ala Thr Arg Ser Lys Val Asn Gly Gln Ser Gly 
225                 230                 235                 240 


Arg Met Asp Phe Phe Trp Thr Ile Leu Lys Pro Asn Asp Ala Ile His 
                245                 250                 255     


Phe Glu Ser Asn Gly Asn Phe Ile Ala Pro Glu Tyr Ala Tyr Lys Ile 
            260                 265                 270         


Val Lys Lys Gly Asp Ser Thr Ile Met Lys Ser Glu Val Glu Tyr Gly 
        275                 280                 285             


Asn Cys Asn Thr Lys Cys Gln Thr Pro Ile Gly Ala Ile Asn Ser Ser 
    290                 295                 300                 


Met Pro Phe His Asn Ile His Pro Leu Thr Ile Gly Glu Cys Pro Lys 
305                 310                 315                 320 


Tyr Val Lys Ser Asn Lys Leu Val Leu Ala Thr Gly Leu Arg Asn Ser 
                325                 330                 335     


Pro Gln Asn Val Pro Gln Arg Glu Thr Arg Gly Leu Phe Gly Ala Ile 
            340                 345                 350         


Ala Gly Phe Ile Glu Gly Gly Trp Gln Gly Met Val Asp Gly Trp Tyr 
        355                 360                 365             


Gly Tyr His His Ser Asn Glu Gln Gly Ser Gly Tyr Ala Ala Asp Lys 
    370                 375                 380                 


Glu Ser Thr Gln Lys Ala Ile Asp Gly Ile Thr Asn Lys Val Asn Ser 
385                 390                 395                 400 


Ile Ile Asp Lys Met Asn Thr Gln Phe Glu Ala Val Gly Lys Glu Phe 
                405                 410                 415     


Asn Asn Leu Glu Arg Arg Ile Glu Asn Leu Asn Lys Lys Met Glu Asp 
            420                 425                 430         


Gly Phe Leu Asp Val Trp Thr Tyr Asn Ala Glu Leu Leu Val Leu Met 
        435                 440                 445             


Glu Asn Glu Arg Thr Leu Asp Phe His Asp Ser Asn Val Lys Asn Leu 
    450                 455                 460                 


Tyr Asp Lys Val Arg Leu Gln Leu Arg Asp Asn Ala Lys Glu Leu Gly 
465                 470                 475                 480 


Asn Gly Cys Phe Glu Phe Tyr His Lys Cys Asp Asn Glu Cys Met Glu 
                485                 490                 495     


Ser Val Arg Asn Gly Thr Tyr Asp Tyr Pro Gln Tyr Ser Glu Glu Ala 
            500                 505                 510         


Arg Leu Asn Arg Glu Glu Ile Ser Gly Val Lys Leu Glu Ser Met Gly 
        515                 520                 525             


Thr Tyr Gln Ile Leu Ser Ile Tyr Ser Thr Val Ala Ser Ser Leu Ala 
    530                 535                 540                 


Leu Ala Ile Met Ile Ala Gly Leu Ser Phe Trp Met Cys Ser Asn Gly 
545                 550                 555                 560 


Ser Leu Gln Cys Arg Ile Cys Ile 
                565             


<210> 4
<211> 571
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 4
Met Glu Lys Ile Val Leu Leu Leu Ala Ile Val Ser Leu Val Lys Ser 
1               5                   10                  15      


Asp Gln Ile Cys Ile Gly Tyr His Ala Asn Asn Ser Thr Glu Gln Val 
            20                  25                  30          


Asp Thr Ile Met Glu Lys Asn Val Thr Val Thr His Ala Gln Asp Ile 
        35                  40                  45              


Leu Glu Lys Thr His Asn Gly Lys Leu Cys Ser Leu Asn Gly Val Lys 
    50                  55                  60                  


Pro Leu Ile Leu Lys Asp Cys Ser Val Ala Gly Trp Leu Leu Gly Asn 
65                  70                  75                  80  


Pro Met Cys Asp Glu Phe Leu Asn Val Pro Glu Trp Ser Tyr Ile Val 
                85                  90                  95      


Glu Lys Asp Asn Pro Ala Asn Gly Leu Cys Tyr Pro Gly Asn Phe Asn 
            100                 105                 110         


Asp Tyr Glu Glu Leu Lys His Leu Leu Ser Ser Thr Asn His Phe Glu 
        115                 120                 125             


Lys Ile Gln Ile Ile Pro Arg Ser Ser Trp Ser Asn His Asp Ala Ser 
    130                 135                 140                 


Ser Gly Val Ser Ser Ala Cys Pro Tyr Asn Gly Arg Ser Ser Phe Phe 
145                 150                 155                 160 


Arg Asn Val Val Trp Leu Ile Lys Lys Asn Asn Ala Tyr Pro Thr Ile 
                165                 170                 175     


Lys Arg Ser Tyr Asn Asn Thr Asn Gln Glu Asp Leu Leu Ile Leu Trp 
            180                 185                 190         


Gly Ile His His Pro Asn Asp Ala Ala Glu Gln Thr Lys Leu Tyr Gln 
        195                 200                 205             


Asn Pro Thr Thr Tyr Val Ser Val Gly Thr Ser Thr Leu Asn Gln Arg 
    210                 215                 220                 


Ser Val Pro Glu Ile Ala Thr Arg Pro Lys Val Asn Gly Gln Ser Gly 
225                 230                 235                 240 


Arg Met Glu Phe Phe Trp Thr Ile Leu Lys Pro Asn Asp Ala Ile Asn 
                245                 250                 255     


Phe Glu Ser Asn Gly Asn Phe Ile Ala Pro Glu Tyr Ala Tyr Lys Ile 
            260                 265                 270         


Val Lys Lys Gly Asp Ser Ala Ile Met Lys Ser Glu Leu Glu Tyr Gly 
        275                 280                 285             


Asn Cys Asn Thr Lys Cys Gln Thr Pro Val Gly Ala Ile Asn Ser Ser 
    290                 295                 300                 


Met Pro Phe His Asn Ile His Pro Leu Thr Ile Gly Glu Cys Pro Lys 
305                 310                 315                 320 


Tyr Val Lys Ser Asn Lys Leu Val Leu Ala Thr Gly Leu Arg Asn Ile 
                325                 330                 335     


Pro Gln Asn Ser Pro Gln Arg Glu Arg Arg Arg Lys Arg Gly Leu Phe 
            340                 345                 350         


Gly Ala Ile Ala Gly Phe Ile Glu Gly Gly Trp Gln Gly Met Val Asp 
        355                 360                 365             


Gly Trp Tyr Gly Tyr His His Ser Asn Glu Gln Gly Ser Gly Tyr Ala 
    370                 375                 380                 


Ala Asp Lys Glu Ser Thr Gln Lys Ala Ile Asp Gly Val Thr Asn Lys 
385                 390                 395                 400 


Val Asn Ser Ile Ile Asp Lys Met Asn Thr Gln Phe Glu Ala Val Gly 
                405                 410                 415     


Arg Glu Phe Asn Asn Leu Glu Arg Arg Ile Glu Asn Leu Asn Lys Lys 
            420                 425                 430         


Met Glu Asp Gly Phe Leu Asp Val Trp Thr Tyr Asn Ala Glu Leu Leu 
        435                 440                 445             


Val Leu Met Glu Asn Glu Arg Thr Leu Asp Phe His Asp Ser Asn Val 
    450                 455                 460                 


Lys Asn Leu Tyr Asp Lys Val Arg Leu Gln Leu Arg Asp Asn Ala Lys 
465                 470                 475                 480 


Glu Leu Gly Asn Gly Cys Phe Glu Phe Tyr His Lys Cys Asp Asn Glu 
                485                 490                 495     


Cys Met Glu Ser Val Arg Asn Gly Thr Tyr Asp Tyr Pro Gln Tyr Ser 
            500                 505                 510         


Glu Glu Ala Arg Leu Lys Arg Glu Glu Ile Ser Gly Val Lys Leu Glu 
        515                 520                 525             


Ser Ile Gly Thr Tyr Gln Ile Leu Ser Ile Tyr Ser Thr Val Ala Ser 
    530                 535                 540                 


Ser Leu Ala Leu Ala Ile Met Val Ala Gly Leu Ser Leu Trp Met Cys 
545                 550                 555                 560 


Ser Asn Gly Ser Leu Gln Cys Arg Ile Cys Ile 
                565                 570     


<210> 5
<211> 571
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 5
Met Glu Asn Ile Val Leu Leu Leu Ala Ile Val Ser Leu Val Lys Ser 
1               5                   10                  15      


Asp Gln Ile Cys Ile Gly Tyr His Ala Asn Asn Ser Thr Glu Gln Val 
            20                  25                  30          


Asp Thr Ile Met Glu Lys Asn Val Thr Val Thr His Ala Gln Asp Ile 
        35                  40                  45              


Leu Glu Lys Thr His Asn Gly Lys Leu Cys Asp Leu Asn Gly Val Lys 
    50                  55                  60                  


Pro Leu Ile Leu Lys Asp Cys Ser Val Ala Gly Trp Leu Leu Gly Asn 
65                  70                  75                  80  


Pro Met Cys Asp Glu Phe Ile Arg Val Pro Glu Trp Ser Tyr Ile Val 
                85                  90                  95      


Glu Arg Ala Asn Pro Ala Asn Asp Leu Cys Tyr Pro Gly Asn Leu Asn 
            100                 105                 110         


Asp Tyr Glu Glu Leu Lys His Leu Leu Ser Arg Ile Asn His Phe Glu 
        115                 120                 125             


Lys Ile Leu Ile Ile Pro Lys Ser Ser Trp Pro Asn His Glu Thr Ser 
    130                 135                 140                 


Leu Gly Val Ser Ala Ala Cys Pro Tyr Gln Gly Thr Pro Ser Phe Phe 
145                 150                 155                 160 


Arg Asn Val Val Trp Leu Ile Lys Lys Asn Asp Ala Tyr Pro Thr Ile 
                165                 170                 175     


Lys Ile Ser Tyr Asn Asn Thr Asn Arg Glu Asp Leu Leu Ile Leu Trp 
            180                 185                 190         


Gly Ile His His Ser Asn Asn Ala Ala Glu Gln Thr Asn Leu Tyr Lys 
        195                 200                 205             


Asn Pro Thr Thr Tyr Ile Ser Val Gly Thr Ser Thr Leu Asn Gln Arg 
    210                 215                 220                 


Leu Val Pro Lys Ile Ala Thr Arg Ser Gln Val Asn Gly Gln Arg Gly 
225                 230                 235                 240 


Arg Met Asp Phe Phe Trp Thr Ile Leu Lys Pro Asn Asp Ala Ile His 
                245                 250                 255     


Phe Glu Ser Asn Gly Asn Phe Ile Ala Pro Glu Tyr Ala Tyr Lys Ile 
            260                 265                 270         


Val Lys Lys Gly Asp Ser Thr Ile Met Lys Ser Glu Val Glu Tyr Gly 
        275                 280                 285             


His Cys Asn Thr Lys Cys Gln Thr Pro Ile Gly Ala Ile Asn Ser Ser 
    290                 295                 300                 


Met Pro Phe His Asn Ile His Pro Leu Thr Ile Gly Glu Cys Pro Lys 
305                 310                 315                 320 


Tyr Val Lys Ser Asn Lys Leu Val Leu Ala Thr Gly Leu Arg Asn Ser 
                325                 330                 335     


Pro Leu Asn Ser Pro Gln Arg Glu Arg Arg Arg Lys Arg Gly Leu Phe 
            340                 345                 350         


Gly Ala Ile Ala Gly Phe Ile Glu Gly Gly Trp Gln Gly Met Val Asp 
        355                 360                 365             


Gly Trp Tyr Gly Tyr His His Ser Asn Glu Gln Gly Ser Gly Tyr Ala 
    370                 375                 380                 


Ala Asp Lys Glu Ser Thr Gln Lys Ala Ile Asp Gly Val Thr Asn Lys 
385                 390                 395                 400 


Val Asn Ser Ile Ile Asp Lys Met Asn Thr Gln Phe Glu Ala Val Gly 
                405                 410                 415     


Arg Glu Phe Asn Asn Leu Glu Arg Arg Ile Glu Asn Leu Asn Lys Lys 
            420                 425                 430         


Met Glu Asp Gly Phe Leu Asp Val Trp Thr Tyr Asn Ala Glu Leu Leu 
        435                 440                 445             


Val Leu Met Glu Asn Glu Arg Thr Leu Asp Phe His Asp Ser Asn Val 
    450                 455                 460                 


Lys Asn Leu Tyr Asp Lys Val Arg Leu Gln Leu Lys Asp Asn Ala Lys 
465                 470                 475                 480 


Glu Leu Gly Asn Gly Cys Phe Glu Phe Tyr His Lys Cys Asn Asn Glu 
                485                 490                 495     


Cys Met Glu Ser Val Arg Asn Gly Thr Tyr Asp Tyr Pro Gln Tyr Ser 
            500                 505                 510         


Glu Glu Ala Arg Leu Lys Arg Glu Glu Ile Ser Gly Val Lys Leu Glu 
        515                 520                 525             


Ser Ile Gly Ile Tyr Gln Ile Leu Ser Ile Tyr Ser Thr Val Ala Ser 
    530                 535                 540                 


Ser Leu Val Leu Ala Ile Met Met Ala Gly Leu Ser Leu Trp Met Cys 
545                 550                 555                 560 


Ser Asn Gly Ser Leu Gln Cys Arg Ile Cys Ile 
                565                 570     


