                         SEQUENCE LISTING

<110>  CU
 
<120>  BISPECIFIC IMMUNOTOXINS TARGETING HUMAN CD25+CCR4+ TUMORS AND 
       TREGS

<130>  104780.0052

<150>  62/807,676
<151>  2019-02-19

<160>  20    

<170>  PatentIn version 3.5

<210>  1
<211>  2391
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CCR4-IL2 bispecific immunotoxin DNA sequence

<400>  1
gctggtgctg acgacgtcgt cgactcctcc aagtccttcg tcatggagaa cttcgcttcc       60

taccacggga ccaagccagg ttacgtcgac tccatccaga agggtatcca gaagccaaag      120

tccggcaccc aaggtaacta cgacgacgac tggaaggggt tctactccac cgacaacaag      180

tacgacgctg cgggatactc tgtagataat gaaaacccgc tctctggaaa agctggaggc      240

gtggtcaagg tcacctaccc aggtctgact aaggtcttgg ctttgaaggt cgacaacgct      300

gagaccatca agaaggagtt gggtttgtcc ttgactgagc cattgatgga gcaagtcggt      360

accgaagagt tcatcaagag attcggtgac ggtgcttcca gagtcgtctt gtccttgcca      420

ttcgctgagg gttcttctag cgttgaatat attaataact gggaacaggc taaggctttg      480

tctgttgaat tggagattaa cttcgaaacc agaggtaaga gaggtcaaga tgcgatgtat      540

gagtatatgg ctcaagcctg tgctggtaac agagtcagac gttctgttgg ttcctctttg      600

tcctgtatca acctagactg ggacgtcatc agagacaaga ctaagaccaa gatcgagtct      660

ttgaaagagc atggcccaat caagaacaag atgtccgaat cccccgctaa gaccgtctcc      720

gaggaaaagg ccaagcaata cctagaagag ttccaccaaa ccgccttgga gcatcctgaa      780

ttgtcagaac ttaaaaccgt tactgggacc aatcctgtat tcgctggggc taactatgcg      840

gcgtgggcag taaacgttgc gcaagttatc gatagcgaaa cagctgataa tttggaaaag      900

acaactgctg ctctttcgat acttcctggt atcggtagcg taatgggcat tgcagacggt      960

gccgttcacc acaatacaga agagatagtg gcacaatcca tcgctttgtc ctctttgatg     1020

gttgctcaag ctatcccatt ggtcggtgag ttggttgaca tcggtttcgc tgcctacaac     1080

ttcgtcgagt ccatcatcaa cttgttccaa gtcgtccaca actcctacaa ccgtccggct     1140

tactccccag gtcacaagac ccaaccattc ttgccatggg gtggtggtgg ttctgacatt     1200

gagttgactc aatctccatc ttccttggct gtttctgctg gtgagaaggt tactatgtct     1260

tgtaagtctt cccaatctat tttgtactct tccaaccaaa agaactactt ggcttggtac     1320

caacaaaagc caggtcaatc tccaaagttg ttgatttact gggcttctac tagagagtct     1380

ggtgttccag acagattcac tggttctggt tctggtactg acttcacttt gactatttct     1440

tccgttcaag ctgaggactt ggctgtttac tactgtcacc aatacttgtc ttcctacact     1500

ttcggtggtg gtactaagtt ggagattaag ggtggtggtg gttctggtgg tggtggatct     1560

ggtggtggtg gttctcaagt tcaattgcaa caatctggtc cagagttggt tagaccaggt     1620

gcttctgtta gaatttcttg taaggcttct ggttacactt tcgcttctta ctacattcaa     1680

tggatgaagc aaagaccagg tcaaggtttg gagtggattg gttggattaa cccaggtaac     1740

gttaacacta agtacaacga gaagttcaag ggtaaggcta ctttgactgc tgacaagtct     1800

tccactaccg cttacatgca attgtcttcc ttgacttctg aggactctgc tgtttacttc     1860

tgtgctagat ccacttacta cagaccattg gactactggg gtcaaggtac taccgttact     1920

gtttcttccg gtggtggtgg ttctggtggt ggtggatccg gtggtggtgg ttctgctcca     1980

acttcttctt ctactaagaa gactcaattg caattggagc acttgttgtt ggacttgcaa     2040

atgattttga acggtattaa caactacaag aacccaaagt tgactagaat gttgactttc     2100

aagttctaca tgccaaagaa ggctactgag ttgaagcact tgcaatgttt ggaggaggaa     2160

ttgaagccat tggaggaagt tttgaacttg gctcaatcta agaacttcca cttgagacca     2220

agagacttga tttctaacat taacgttatt gttttggagt tgaagggttc tgagactact     2280

ttcatgtgtg agtacgctga cgagactgct actattgttg agttcttgaa cagatggatt     2340

actttctgtc aatctattat ctctactttg actcaccacc accaccacca c              2391


<210>  2
<211>  797
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CCR4-IL2 bispecific immunotoxin amino acid sequence

<400>  2

Ala Gly Ala Asp Asp Val Val Asp Ser Ser Lys Ser Phe Val Met Glu 
1               5                   10                  15      


Asn Phe Ala Ser Tyr His Gly Thr Lys Pro Gly Tyr Val Asp Ser Ile 
            20                  25                  30          


Gln Lys Gly Ile Gln Lys Pro Lys Ser Gly Thr Gln Gly Asn Tyr Asp 
        35                  40                  45              


Asp Asp Trp Lys Gly Phe Tyr Ser Thr Asp Asn Lys Tyr Asp Ala Ala 
    50                  55                  60                  


Gly Tyr Ser Val Asp Asn Glu Asn Pro Leu Ser Gly Lys Ala Gly Gly 
65                  70                  75                  80  


Val Val Lys Val Thr Tyr Pro Gly Leu Thr Lys Val Leu Ala Leu Lys 
                85                  90                  95      


Val Asp Asn Ala Glu Thr Ile Lys Lys Glu Leu Gly Leu Ser Leu Thr 
            100                 105                 110         


Glu Pro Leu Met Glu Gln Val Gly Thr Glu Glu Phe Ile Lys Arg Phe 
        115                 120                 125             


Gly Asp Gly Ala Ser Arg Val Val Leu Ser Leu Pro Phe Ala Glu Gly 
    130                 135                 140                 


Ser Ser Ser Val Glu Tyr Ile Asn Asn Trp Glu Gln Ala Lys Ala Leu 
145                 150                 155                 160 


Ser Val Glu Leu Glu Ile Asn Phe Glu Thr Arg Gly Lys Arg Gly Gln 
                165                 170                 175     


Asp Ala Met Tyr Glu Tyr Met Ala Gln Ala Cys Ala Gly Asn Arg Val 
            180                 185                 190         


Arg Arg Ser Val Gly Ser Ser Leu Ser Cys Ile Asn Leu Asp Trp Asp 
        195                 200                 205             


Val Ile Arg Asp Lys Thr Lys Thr Lys Ile Glu Ser Leu Lys Glu His 
    210                 215                 220                 


Gly Pro Ile Lys Asn Lys Met Ser Glu Ser Pro Ala Lys Thr Val Ser 
225                 230                 235                 240 


Glu Glu Lys Ala Lys Gln Tyr Leu Glu Glu Phe His Gln Thr Ala Leu 
                245                 250                 255     


Glu His Pro Glu Leu Ser Glu Leu Lys Thr Val Thr Gly Thr Asn Pro 
            260                 265                 270         


Val Phe Ala Gly Ala Asn Tyr Ala Ala Trp Ala Val Asn Val Ala Gln 
        275                 280                 285             


Val Ile Asp Ser Glu Thr Ala Asp Asn Leu Glu Lys Thr Thr Ala Ala 
    290                 295                 300                 


Leu Ser Ile Leu Pro Gly Ile Gly Ser Val Met Gly Ile Ala Asp Gly 
305                 310                 315                 320 


Ala Val His His Asn Thr Glu Glu Ile Val Ala Gln Ser Ile Ala Leu 
                325                 330                 335     


Ser Ser Leu Met Val Ala Gln Ala Ile Pro Leu Val Gly Glu Leu Val 
            340                 345                 350         


Asp Ile Gly Phe Ala Ala Tyr Asn Phe Val Glu Ser Ile Ile Asn Leu 
        355                 360                 365             


Phe Gln Val Val His Asn Ser Tyr Asn Arg Pro Ala Tyr Ser Pro Gly 
    370                 375                 380                 


His Lys Thr Gln Pro Phe Leu Pro Trp Gly Gly Gly Gly Ser Asp Ile 
385                 390                 395                 400 


Glu Leu Thr Gln Ser Pro Ser Ser Leu Ala Val Ser Ala Gly Glu Lys 
                405                 410                 415     


Val Thr Met Ser Cys Lys Ser Ser Gln Ser Ile Leu Tyr Ser Ser Asn 
            420                 425                 430         


Gln Lys Asn Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ser Pro 
        435                 440                 445             


Lys Leu Leu Ile Tyr Trp Ala Ser Thr Arg Glu Ser Gly Val Pro Asp 
    450                 455                 460                 


Arg Phe Thr Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser 
465                 470                 475                 480 


Ser Val Gln Ala Glu Asp Leu Ala Val Tyr Tyr Cys His Gln Tyr Leu 
                485                 490                 495     


Ser Ser Tyr Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Gly Gly 
            500                 505                 510         


Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Val Gln 
        515                 520                 525             


Leu Gln Gln Ser Gly Pro Glu Leu Val Arg Pro Gly Ala Ser Val Arg 
    530                 535                 540                 


Ile Ser Cys Lys Ala Ser Gly Tyr Thr Phe Ala Ser Tyr Tyr Ile Gln 
545                 550                 555                 560 


Trp Met Lys Gln Arg Pro Gly Gln Gly Leu Glu Trp Ile Gly Trp Ile 
                565                 570                 575     


Asn Pro Gly Asn Val Asn Thr Lys Tyr Asn Glu Lys Phe Lys Gly Lys 
            580                 585                 590         


Ala Thr Leu Thr Ala Asp Lys Ser Ser Thr Thr Ala Tyr Met Gln Leu 
        595                 600                 605             


Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys Ala Arg Ser 
    610                 615                 620                 


Thr Tyr Tyr Arg Pro Leu Asp Tyr Trp Gly Gln Gly Thr Thr Val Thr 
625                 630                 635                 640 


Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
                645                 650                 655     


Gly Ser Ala Pro Thr Ser Ser Ser Thr Lys Lys Thr Gln Leu Gln Leu 
            660                 665                 670         


Glu His Leu Leu Leu Asp Leu Gln Met Ile Leu Asn Gly Ile Asn Asn 
        675                 680                 685             


Tyr Lys Asn Pro Lys Leu Thr Arg Met Leu Thr Phe Lys Phe Tyr Met 
    690                 695                 700                 


Pro Lys Lys Ala Thr Glu Leu Lys His Leu Gln Cys Leu Glu Glu Glu 
705                 710                 715                 720 


Leu Lys Pro Leu Glu Glu Val Leu Asn Leu Ala Gln Ser Lys Asn Phe 
                725                 730                 735     


His Leu Arg Pro Arg Asp Leu Ile Ser Asn Ile Asn Val Ile Val Leu 
            740                 745                 750         


Glu Leu Lys Gly Ser Glu Thr Thr Phe Met Cys Glu Tyr Ala Asp Glu 
        755                 760                 765             


Thr Ala Thr Ile Val Glu Phe Leu Asn Arg Trp Ile Thr Phe Cys Gln 
    770                 775                 780                 


Ser Ile Ile Ser Thr Leu Thr His His His His His His 
785                 790                 795         


<210>  3
<211>  2391
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IL2-CCR4 bispecific immunotoxin DNA sequence

<400>  3
gctggtgctg acgacgtcgt cgactcctcc aagtccttcg tcatggagaa cttcgcttcc       60

taccacggga ccaagccagg ttacgtcgac tccatccaga agggtatcca gaagccaaag      120

tccggcaccc aaggtaacta cgacgacgac tggaaggggt tctactccac cgacaacaag      180

tacgacgctg cgggatactc tgtagataat gaaaacccgc tctctggaaa agctggaggc      240

gtggtcaagg tcacctaccc aggtctgact aaggtcttgg ctttgaaggt cgacaacgct      300

gagaccatca agaaggagtt gggtttgtcc ttgactgagc cattgatgga gcaagtcggt      360

accgaagagt tcatcaagag attcggtgac ggtgcttcca gagtcgtctt gtccttgcca      420

ttcgctgagg gttcttctag cgttgaatat attaataact gggaacaggc taaggctttg      480

tctgttgaat tggagattaa cttcgaaacc agaggtaaga gaggtcaaga tgcgatgtat      540

gagtatatgg ctcaagcctg tgctggtaac agagtcagac gttctgttgg ttcctctttg      600

tcctgtatca acctagactg ggacgtcatc agagacaaga ctaagaccaa gatcgagtct      660

ttgaaagagc atggcccaat caagaacaag atgtccgaat cccccgctaa gaccgtctcc      720

gaggaaaagg ccaagcaata cctagaagag ttccaccaaa ccgccttgga gcatcctgaa      780

ttgtcagaac ttaaaaccgt tactgggacc aatcctgtat tcgctggggc taactatgcg      840

gcgtgggcag taaacgttgc gcaagttatc gatagcgaaa cagctgataa tttggaaaag      900

acaactgctg ctctttcgat acttcctggt atcggtagcg taatgggcat tgcagacggt      960

gccgttcacc acaatacaga agagatagtg gcacaatcca tcgctttgtc ctctttgatg     1020

gttgctcaag ctatcccatt ggtcggtgag ttggttgaca tcggtttcgc tgcctacaac     1080

ttcgtcgagt ccatcatcaa cttgttccaa gtcgtccaca actcctacaa ccgtccggct     1140

tactccccag gtcacaagac ccaaccattc ttgccatggg gtggtggtgg ttctgctcca     1200

acttcttctt ctactaagaa gactcaattg caattggagc acttgttgtt ggacttgcaa     1260

atgattttga acggtattaa caactacaag aacccaaagt tgactagaat gttgactttc     1320

aagttctaca tgccaaagaa ggctactgag ttgaagcact tgcaatgttt ggaggaggaa     1380

ttgaagccat tggaggaagt tttgaacttg gctcaatcta agaacttcca cttgagacca     1440

agagacttga tttctaacat taacgttatt gttttggagt tgaagggttc tgagactact     1500

ttcatgtgtg agtacgctga cgagactgct actattgttg agttcttgaa cagatggatt     1560

actttctgtc aatctattat ctctactttg actggtggtg gtggttctgg tggtggtgga     1620

tccggtggtg gtggttctga cattgagttg actcaatctc catcttcctt ggctgtttct     1680

gctggtgaga aggttactat gtcttgtaag tcttcccaat ctattttgta ctcttccaac     1740

caaaagaact acttggcttg gtaccaacaa aagccaggtc aatctccaaa gttgttgatt     1800

tactgggctt ctactagaga gtctggtgtt ccagacagat tcactggttc tggttctggt     1860

actgacttca ctttgactat ttcttccgtt caagctgagg acttggctgt ttactactgt     1920

caccaatact tgtcttccta cactttcggt ggtggtacta agttggagat taagggtggt     1980

ggtggttctg gtggtggtgg atctggtggt ggtggttctc aagttcaatt gcaacaatct     2040

ggtccagagt tggttagacc aggtgcttct gttagaattt cttgtaaggc ttctggttac     2100

actttcgctt cttactacat tcaatggatg aagcaaagac caggtcaagg tttggagtgg     2160

attggttgga ttaacccagg taacgttaac actaagtaca acgagaagtt caagggtaag     2220

gctactttga ctgctgacaa gtcttccact accgcttaca tgcaattgtc ttccttgact     2280

tctgaggact ctgctgttta cttctgtgct agatccactt actacagacc attggactac     2340

tggggtcaag gtactaccgt tactgtttct tcccaccacc accaccacca c              2391


<210>  4
<211>  797
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IL2-CCR4 bispecific immunotoxin amino acid sequence

<400>  4

Ala Gly Ala Asp Asp Val Val Asp Ser Ser Lys Ser Phe Val Met Glu 
1               5                   10                  15      


Asn Phe Ala Ser Tyr His Gly Thr Lys Pro Gly Tyr Val Asp Ser Ile 
            20                  25                  30          


Gln Lys Gly Ile Gln Lys Pro Lys Ser Gly Thr Gln Gly Asn Tyr Asp 
        35                  40                  45              


Asp Asp Trp Lys Gly Phe Tyr Ser Thr Asp Asn Lys Tyr Asp Ala Ala 
    50                  55                  60                  


Gly Tyr Ser Val Asp Asn Glu Asn Pro Leu Ser Gly Lys Ala Gly Gly 
65                  70                  75                  80  


Val Val Lys Val Thr Tyr Pro Gly Leu Thr Lys Val Leu Ala Leu Lys 
                85                  90                  95      


Val Asp Asn Ala Glu Thr Ile Lys Lys Glu Leu Gly Leu Ser Leu Thr 
            100                 105                 110         


Glu Pro Leu Met Glu Gln Val Gly Thr Glu Glu Phe Ile Lys Arg Phe 
        115                 120                 125             


Gly Asp Gly Ala Ser Arg Val Val Leu Ser Leu Pro Phe Ala Glu Gly 
    130                 135                 140                 


Ser Ser Ser Val Glu Tyr Ile Asn Asn Trp Glu Gln Ala Lys Ala Leu 
145                 150                 155                 160 


Ser Val Glu Leu Glu Ile Asn Phe Glu Thr Arg Gly Lys Arg Gly Gln 
                165                 170                 175     


Asp Ala Met Tyr Glu Tyr Met Ala Gln Ala Cys Ala Gly Asn Arg Val 
            180                 185                 190         


Arg Arg Ser Val Gly Ser Ser Leu Ser Cys Ile Asn Leu Asp Trp Asp 
        195                 200                 205             


Val Ile Arg Asp Lys Thr Lys Thr Lys Ile Glu Ser Leu Lys Glu His 
    210                 215                 220                 


Gly Pro Ile Lys Asn Lys Met Ser Glu Ser Pro Ala Lys Thr Val Ser 
225                 230                 235                 240 


Glu Glu Lys Ala Lys Gln Tyr Leu Glu Glu Phe His Gln Thr Ala Leu 
                245                 250                 255     


Glu His Pro Glu Leu Ser Glu Leu Lys Thr Val Thr Gly Thr Asn Pro 
            260                 265                 270         


Val Phe Ala Gly Ala Asn Tyr Ala Ala Trp Ala Val Asn Val Ala Gln 
        275                 280                 285             


Val Ile Asp Ser Glu Thr Ala Asp Asn Leu Glu Lys Thr Thr Ala Ala 
    290                 295                 300                 


Leu Ser Ile Leu Pro Gly Ile Gly Ser Val Met Gly Ile Ala Asp Gly 
305                 310                 315                 320 


Ala Val His His Asn Thr Glu Glu Ile Val Ala Gln Ser Ile Ala Leu 
                325                 330                 335     


Ser Ser Leu Met Val Ala Gln Ala Ile Pro Leu Val Gly Glu Leu Val 
            340                 345                 350         


Asp Ile Gly Phe Ala Ala Tyr Asn Phe Val Glu Ser Ile Ile Asn Leu 
        355                 360                 365             


Phe Gln Val Val His Asn Ser Tyr Asn Arg Pro Ala Tyr Ser Pro Gly 
    370                 375                 380                 


His Lys Thr Gln Pro Phe Leu Pro Trp Gly Gly Gly Gly Ser Ala Pro 
385                 390                 395                 400 


Thr Ser Ser Ser Thr Lys Lys Thr Gln Leu Gln Leu Glu His Leu Leu 
                405                 410                 415     


Leu Asp Leu Gln Met Ile Leu Asn Gly Ile Asn Asn Tyr Lys Asn Pro 
            420                 425                 430         


Lys Leu Thr Arg Met Leu Thr Phe Lys Phe Tyr Met Pro Lys Lys Ala 
        435                 440                 445             


Thr Glu Leu Lys His Leu Gln Cys Leu Glu Glu Glu Leu Lys Pro Leu 
    450                 455                 460                 


Glu Glu Val Leu Asn Leu Ala Gln Ser Lys Asn Phe His Leu Arg Pro 
465                 470                 475                 480 


Arg Asp Leu Ile Ser Asn Ile Asn Val Ile Val Leu Glu Leu Lys Gly 
                485                 490                 495     


Ser Glu Thr Thr Phe Met Cys Glu Tyr Ala Asp Glu Thr Ala Thr Ile 
            500                 505                 510         


Val Glu Phe Leu Asn Arg Trp Ile Thr Phe Cys Gln Ser Ile Ile Ser 
        515                 520                 525             


Thr Leu Thr Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
    530                 535                 540                 


Gly Ser Asp Ile Glu Leu Thr Gln Ser Pro Ser Ser Leu Ala Val Ser 
545                 550                 555                 560 


Ala Gly Glu Lys Val Thr Met Ser Cys Lys Ser Ser Gln Ser Ile Leu 
                565                 570                 575     


Tyr Ser Ser Asn Gln Lys Asn Tyr Leu Ala Trp Tyr Gln Gln Lys Pro 
            580                 585                 590         


Gly Gln Ser Pro Lys Leu Leu Ile Tyr Trp Ala Ser Thr Arg Glu Ser 
        595                 600                 605             


Gly Val Pro Asp Arg Phe Thr Gly Ser Gly Ser Gly Thr Asp Phe Thr 
    610                 615                 620                 


Leu Thr Ile Ser Ser Val Gln Ala Glu Asp Leu Ala Val Tyr Tyr Cys 
625                 630                 635                 640 


His Gln Tyr Leu Ser Ser Tyr Thr Phe Gly Gly Gly Thr Lys Leu Glu 
                645                 650                 655     


Ile Lys Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
            660                 665                 670         


Ser Gln Val Gln Leu Gln Gln Ser Gly Pro Glu Leu Val Arg Pro Gly 
        675                 680                 685             


Ala Ser Val Arg Ile Ser Cys Lys Ala Ser Gly Tyr Thr Phe Ala Ser 
    690                 695                 700                 


Tyr Tyr Ile Gln Trp Met Lys Gln Arg Pro Gly Gln Gly Leu Glu Trp 
705                 710                 715                 720 


Ile Gly Trp Ile Asn Pro Gly Asn Val Asn Thr Lys Tyr Asn Glu Lys 
                725                 730                 735     


Phe Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Thr Thr Ala 
            740                 745                 750         


Tyr Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe 
        755                 760                 765             


Cys Ala Arg Ser Thr Tyr Tyr Arg Pro Leu Asp Tyr Trp Gly Gln Gly 
    770                 775                 780                 


Thr Thr Val Thr Val Ser Ser His His His His His His 
785                 790                 795         


<210>  5
<211>  230
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Rearranged mouse variable heavy (VH) and variable light (VL) K 
       domains of mAb1567

<400>  5

Gln Val Gln Leu Gln Gln Ser Gly Pro Glu Leu Val Arg Pro Gly Ala 
1               5                   10                  15      


Ser Val Arg Ile Ser Cys Lys Ala Ser Gly Tyr Thr Phe Ala Ser Tyr 
            20                  25                  30          


Tyr Ile Gln Trp Met Lys Gln Arg Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Trp Ile Asn Pro Gly Asn Val Asn Thr Lys Tyr Asn Glu Lys Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Thr Thr Ala Tyr 
65                  70                  75                  80  


Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys 
                85                  90                  95      


Ala Arg Ser Thr Tyr Tyr Arg Pro Leu Asp Tyr Trp Gly Gln Gly Thr 
            100                 105                 110         


Thr Val Thr Val Ser Ser Asp Ile Glu Leu Thr Gln Ser Pro Ser Ser 
        115                 120                 125             


Leu Ala Val Ser Ala Gly Glu Lys Val Thr Met Ser Cys Lys Ser Ser 
    130                 135                 140                 


Gln Ser Ile Leu Tyr Ser Ser Asn Gln Lys Asn Tyr Leu Ala Trp Tyr 
145                 150                 155                 160 


Gln Gln Lys Pro Gly Gln Ser Pro Lys Leu Leu Ile Tyr Trp Ala Ser 
                165                 170                 175     


Thr Arg Glu Ser Gly Val Pro Asp Arg Phe Thr Gly Ser Gly Ser Gly 
            180                 185                 190         


Thr Asp Phe Thr Leu Thr Ile Ser Ser Val Gln Ala Glu Asp Leu Ala 
        195                 200                 205             


Val Tyr Tyr Cys His Gln Tyr Leu Ser Ser Tyr Thr Phe Gly Gly Gly 
    210                 215                 220                 


Thr Lys Leu Glu Ile Lys 
225                 230 


<210>  6
<211>  230
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Humanized variable heavy (VH) and variable light (VK) K domains 
       of mAb1567

<400>  6

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Ala Ser Tyr 
            20                  25                  30          


Tyr Met His Trp Met Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Trp Ile Asn Pro Gly Asn Val Asn Thr Lys Tyr Asn Glu Lys Phe 
    50                  55                  60                  


Lys Gly Arg Ala Thr Leu Thr Val Asp Thr Ser Thr Asn Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Ser Thr Tyr Tyr Arg Pro Leu Asp Tyr Trp Gly Gln Gly Thr 
            100                 105                 110         


Leu Val Thr Val Ser Ser Asp Ile Val Met Thr Gln Ser Pro Asp Ser 
        115                 120                 125             


Leu Ala Val Ser Leu Gly Glu Arg Ala Thr Ile Asn Cys Lys Ser Ser 
    130                 135                 140                 


Gln Ser Ile Leu Tyr Ser Ser Asn Gln Lys Asn Tyr Leu Ala Trp Tyr 
145                 150                 155                 160 


Gln Gln Lys Pro Gly Gln Ser Pro Lys Leu Leu Ile Tyr Trp Ala Ser 
                165                 170                 175     


Thr Arg Glu Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly 
            180                 185                 190         


Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Ala Glu Asp Val Ala 
        195                 200                 205             


Val Tyr Tyr Cys His Gln Tyr Leu Ser Ser Tyr Thr Phe Gly Gln Gly 
    210                 215                 220                 


Thr Lys Leu Glu Ile Lys 
225                 230 


<210>  7
<211>  1179
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DT 390 DNA sequence

<400>  7
gctggtgctg acgacgtcgt cgactcctcc aagtccttcg tcatggagaa cttcgcttcc       60

taccacggga ccaagccagg ttacgtcgac tccatccaga agggtatcca gaagccaaag      120

tccggcaccc aaggtaacta cgacgacgac tggaaggggt tctactccac cgacaacaag      180

tacgacgctg cgggatactc tgtagataat gaaaacccgc tctctggaaa agctggaggc      240

gtggtcaagg tcacctaccc aggtctgact aaggtcttgg ctttgaaggt cgacaacgct      300

gagaccatca agaaggagtt gggtttgtcc ttgactgagc cattgatgga gcaagtcggt      360

accgaagagt tcatcaagag attcggtgac ggtgcttcca gagtcgtctt gtccttgcca      420

ttcgctgagg gttcttctag cgttgaatat attaataact gggaacaggc taaggctttg      480

tctgttgaat tggagattaa cttcgaaacc agaggtaaga gaggtcaaga tgcgatgtat      540

gagtatatgg ctcaagcctg tgctggtaac agagtcagac gttctgttgg ttcctctttg      600

tcctgtatca acctagactg ggacgtcatc agagacaaga ctaagaccaa gatcgagtct      660

ttgaaagagc atggcccaat caagaacaag atgtccgaat cccccgctaa gaccgtctcc      720

gaggaaaagg ccaagcaata cctagaagag ttccaccaaa ccgccttgga gcatcctgaa      780

ttgtcagaac ttaaaaccgt tactgggacc aatcctgtat tcgctggggc taactatgcg      840

gcgtgggcag taaacgttgc gcaagttatc gatagcgaaa cagctgataa tttggaaaag      900

acaactgctg ctctttcgat acttcctggt atcggtagcg taatgggcat tgcagacggt      960

gccgttcacc acaatacaga agagatagtg gcacaatcca tcgctttgtc ctctttgatg     1020

gttgctcaag ctatcccatt ggtcggtgag ttggttgaca tcggtttcgc tgcctacaac     1080

ttcgtcgagt ccatcatcaa cttgttccaa gtcgtccaca actcctacaa ccgtccggct     1140

tactccccag gtcacaagac ccaaccattc ttgccatgg                            1179


<210>  8
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Linker sequence

<400>  8
ggtggtggtg gttct                                                        15


<210>  9
<211>  336
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  anti-human CCR4 scFv VL

<400>  9
gacattgagt tgactcaatc tccatcttcc ttggctgttt ctgctggtga gaaggttact       60

atgtcttgta agtcttccca atctattttg tactcttcca accaaaagaa ctacttggct      120

tggtaccaac aaaagccagg tcaatctcca aagttgttga tttactgggc ttctactaga      180

gagtctggtg ttccagacag attcactggt tctggttctg gtactgactt cactttgact      240

atttcttccg ttcaagctga ggacttggct gtttactact gtcaccaata cttgtcttcc      300

tacactttcg gtggtggtac taagttggag attaag                                336


<210>  10
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  linker sequence

<400>  10
ggtggtggtg gttctggtgg tggtggatct ggtggtggtg gttct                       45


<210>  11
<211>  354
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  anti-human CCR4 scFv VH

<400>  11
caagttcaat tgcaacaatc tggtccagag ttggttagac caggtgcttc tgttagaatt       60

tcttgtaagg cttctggtta cactttcgct tcttactaca ttcaatggat gaagcaaaga      120

ccaggtcaag gtttggagtg gattggttgg attaacccag gtaacgttaa cactaagtac      180

aacgagaagt tcaagggtaa ggctactttg actgctgaca agtcttccac taccgcttac      240

atgcaattgt cttccttgac ttctgaggac tctgctgttt acttctgtgc tagatccact      300

tactacagac cattggacta ctggggtcaa ggtactaccg ttactgtttc ttcc            354


<210>  12
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  linker sequence

<400>  12
ggtggtggtg gttctggtgg tggtggatcc ggtggtggtg gttct                       45


<210>  13
<211>  417
<212>  DNA
<213>  Homo sapiens

<400>  13
gctccaactt cttcttctac taagaagact caattgcaat tggagcactt gttgttggac       60

ttgcaaatga ttttgaacgg tattaacaac tacaagaacc caaagttgac tagaatgttg      120

actttcaagt tctacatgcc aaagaaggct actgagttga agcacttgca atgtttggag      180

gaggaattga agccattgga ggaagttttg aacttggctc aatctaagaa cttccacttg      240

agaccaagag acttgatttc taacattaac gttattgttt tggagttgaa gggttctgag      300

actactttca tgtgtgagta cgctgacgag actgctacta ttgttgagtt cttgaacaga      360

tggattactt tctgtcaatc tattatctct actttgactc accaccacca ccaccac         417


<210>  14
<211>  1179
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DT390

<400>  14
gctggtgctg acgacgtcgt cgactcctcc aagtccttcg tcatggagaa cttcgcttcc       60

taccacggga ccaagccagg ttacgtcgac tccatccaga agggtatcca gaagccaaag      120

tccggcaccc aaggtaacta cgacgacgac tggaaggggt tctactccac cgacaacaag      180

tacgacgctg cgggatactc tgtagataat gaaaacccgc tctctggaaa agctggaggc      240

gtggtcaagg tcacctaccc aggtctgact aaggtcttgg ctttgaaggt cgacaacgct      300

gagaccatca agaaggagtt gggtttgtcc ttgactgagc cattgatgga gcaagtcggt      360

accgaagagt tcatcaagag attcggtgac ggtgcttcca gagtcgtctt gtccttgcca      420

ttcgctgagg gttcttctag cgttgaatat attaataact gggaacaggc taaggctttg      480

tctgttgaat tggagattaa cttcgaaacc agaggtaaga gaggtcaaga tgcgatgtat      540

gagtatatgg ctcaagcctg tgctggtaac agagtcagac gttctgttgg ttcctctttg      600

tcctgtatca acctagactg ggacgtcatc agagacaaga ctaagaccaa gatcgagtct      660

ttgaaagagc atggcccaat caagaacaag atgtccgaat cccccgctaa gaccgtctcc      720

gaggaaaagg ccaagcaata cctagaagag ttccaccaaa ccgccttgga gcatcctgaa      780

ttgtcagaac ttaaaaccgt tactgggacc aatcctgtat tcgctggggc taactatgcg      840

gcgtgggcag taaacgttgc gcaagttatc gatagcgaaa cagctgataa tttggaaaag      900

acaactgctg ctctttcgat acttcctggt atcggtagcg taatgggcat tgcagacggt      960

gccgttcacc acaatacaga agagatagtg gcacaatcca tcgctttgtc ctctttgatg     1020

gttgctcaag ctatcccatt ggtcggtgag ttggttgaca tcggtttcgc tgcctacaac     1080

ttcgtcgagt ccatcatcaa cttgttccaa gtcgtccaca actcctacaa ccgtccggct     1140

tactccccag gtcacaagac ccaaccattc ttgccatgg                            1179


<210>  15
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  linker sequence

<400>  15
ggtggtggtg gttct                                                        15


<210>  16
<211>  399
<212>  DNA
<213>  Homo sapiens

<400>  16
gctccaactt cttcttctac taagaagact caattgcaat tggagcactt gttgttggac       60

ttgcaaatga ttttgaacgg tattaacaac tacaagaacc caaagttgac tagaatgttg      120

actttcaagt tctacatgcc aaagaaggct actgagttga agcacttgca atgtttggag      180

gaggaattga agccattgga ggaagttttg aacttggctc aatctaagaa cttccacttg      240

agaccaagag acttgatttc taacattaac gttattgttt tggagttgaa gggttctgag      300

actactttca tgtgtgagta cgctgacgag actgctacta ttgttgagtt cttgaacaga      360

tggattactt tctgtcaatc tattatctct actttgact                             399


<210>  17
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  linker sequence

<400>  17
ggtggtggtg gttctggtgg tggtggatcc ggtggtggtg gttct                       45


<210>  18
<211>  336
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  anti-human CCR4 scFv VL

<400>  18
gacattgagt tgactcaatc tccatcttcc ttggctgttt ctgctggtga gaaggttact       60

atgtcttgta agtcttccca atctattttg tactcttcca accaaaagaa ctacttggct      120

tggtaccaac aaaagccagg tcaatctcca aagttgttga tttactgggc ttctactaga      180

gagtctggtg ttccagacag attcactggt tctggttctg gtactgactt cactttgact      240

atttcttccg ttcaagctga ggacttggct gtttactact gtcaccaata cttgtcttcc      300

tacactttcg gtggtggtac taagttggag attaag                                336


<210>  19
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  linker sequence

<400>  19
ggtggtggtg gttctggtgg tggtggatct ggtggtggtg gttct                       45


<210>  20
<211>  372
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  anti-human CCR4 scFv VH

<400>  20
caagttcaat tgcaacaatc tggtccagag ttggttagac caggtgcttc tgttagaatt       60

tcttgtaagg cttctggtta cactttcgct tcttactaca ttcaatggat gaagcaaaga      120

ccaggtcaag gtttggagtg gattggttgg attaacccag gtaacgttaa cactaagtac      180

aacgagaagt tcaagggtaa ggctactttg actgctgaca agtcttccac taccgcttac      240

atgcaattgt cttccttgac ttctgaggac tctgctgttt acttctgtgc tagatccact      300

tactacagac cattggacta ctggggtcaa ggtactaccg ttactgtttc ttcccaccac      360

caccaccacc ac                                                          372


