                         SEQUENCE LISTING

<110>  Pioneer Biolabs, LLC
 
<120>  METHODS AND COMPOSITIONS FOR GENERATING CRISPR GUIDE RNA 
       LIBRARIES

<130>  PIO100

<160>  597   

<170>  PatentIn version 3.5

<210>  1
<211>  81
<212>  DNA
<213>  Streptococcus pyogenes

<400>  1
gttttagagc tagaaatagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt       60

ggcaccgagt cggtgctttt t                                                 81


<210>  2
<211>  81
<212>  DNA
<213>  Streptococcus pyogenes

<400>  2
aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct tattttaact       60

tgctatttct agctctaaaa c                                                 81


<210>  3
<211>  81
<212>  RNA
<213>  Streptococcus pyogenes

<400>  3
guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc cguuaucaac uugaaaaagu       60

ggcaccgagu cggugcuuuu u                                                 81


<210>  4
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  4
gtyggagagc tagaaatagc aagttccaat aaggctagtc cgttatcaac ttgaaaaagt       60

ggcaccgagt cggtgctttt t                                                 81


<210>  5
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  5
aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct tattggaact       60

tgctatttct agctctccra c                                                 81


<210>  6
<211>  101
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  n is a, c, g, or t

<400>  6
nnnnnnnnnn nnnnnnnnnn gtyggagagc tagaaatagc aagttccaat aaggctagtc       60

cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt t                          101


<210>  7
<211>  101
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (82)..(101)
<223>  n is a, c, g, or t

<400>  7
aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct tattggaact       60

tgctatttct agctctccra cnnnnnnnnn nnnnnnnnnn n                          101


<210>  8
<211>  101
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  n is a, c, g, or u

<400>  8
nnnnnnnnnn nnnnnnnnnn guyggagagc uagaaauagc aaguuccaau aaggcuaguc       60

cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu u                          101


<210>  9
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  9
ggtyggaagc tagaaatagc aagtccaact aaggctagtc cgttatcaac ttgaaaaagt       60

ggcaccgagt cggtgctttt t                                                 81


<210>  10
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  10
aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct tagttggact       60

tgctatttct agcttccrac c                                                 81


<210>  11
<211>  100
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (1)..(19)
<223>  n is a, c, g, or t

<400>  11
nnnnnnnnnn nnnnnnnnng gtyggaagct agaaatagca agtccaacta aggctagtcc       60

gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt                            100


<210>  12
<211>  100
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (82)..(100)
<223>  n is a, c, g, or t

<400>  12
aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct tagttggact       60

tgctatttct agcttccrac cnnnnnnnnn nnnnnnnnnn                            100


<210>  13
<211>  100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (1)..(19)
<223>  n is a, c, g, or u

<400>  13
nnnnnnnnnn nnnnnnnnng guyggaagcu agaaauagca aguccaacua aggcuagucc       60

guuaucaacu ugaaaaagug gcaccgaguc ggugcuuuuu                            100


<210>  14
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  14
gtgtyggagc tagaaatagc aagtcaacat aaggctagtc cgttatcaac ttgaaaaagt       60

ggcaccgagt cggtgctttt t                                                 81


<210>  15
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  15
aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct tatgttgact       60

tgctatttct agctccraca c                                                 81


<210>  16
<211>  99
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (1)..(18)
<223>  n is a, c, g, or t

<400>  16
nnnnnnnnnn nnnnnnnngt gtyggagcta gaaatagcaa gtcaacataa ggctagtccg       60

ttatcaactt gaaaaagtgg caccgagtcg gtgcttttt                              99


<210>  17
<211>  99
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (82)..(99)
<223>  n is a, c, g, or t

<400>  17
aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct tatgttgact       60

tgctatttct agctccraca cnnnnnnnnn nnnnnnnnn                              99


<210>  18
<211>  99
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (1)..(18)
<223>  n is a, c, g, or u

<400>  18
nnnnnnnnnn nnnnnnnngu guyggagcua gaaauagcaa gucaacauaa ggcuaguccg       60

uuaucaacuu gaaaaagugg caccgagucg gugcuuuuu                              99


<210>  19
<211>  82
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  19
ccggtyggag ctagaaatag caagtcaacc taaggctagt ccgttatcaa cttgaaaaag       60

tggcaccgag tcggtgcttt tt                                                82


<210>  20
<211>  82
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  20
aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct taggttgact       60

tgctatttct agctccracc gg                                                82


<210>  21
<211>  99
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (1)..(18)
<223>  n is a, c, g, or t

<400>  21
nnnnnnnnnn nnnnnnnncg gtyggagcta gaaatagcaa gtcaacctaa ggctagtccg       60

ttatcaactt gaaaaagtgg caccgagtcg gtgcttttt                              99


<210>  22
<211>  99
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (82)..(99)
<223>  n is a, c, g, or t

<400>  22
aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct taggttgact       60

tgctatttct agctccracc gnnnnnnnnn nnnnnnnnn                              99


<210>  23
<211>  99
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (1)..(18)
<223>  n is a, c, g, or u

<400>  23
nnnnnnnnnn nnnnnnnncg guyggagcua gaaauagcaa gucaaccuaa ggcuaguccg       60

uuaucaacuu gaaaaagugg caccgagucg gugcuuuuu                              99


<210>  24
<211>  83
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  n is a, c, g, or t

<400>  24
ccnggtygga gctagaaata gcaagtcaac ctaaggctag tccgttatca acttgaaaaa       60

gtggcaccga gtcggtgctt ttt                                               83


<210>  25
<211>  83
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (81)..(81)
<223>  n is a, c, g, or t

<400>  25
aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct taggttgact       60

tgctatttct agctccracc ngg                                               83


<210>  26
<211>  99
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (1)..(19)
<223>  n is a, c, g, or t

<400>  26
nnnnnnnnnn nnnnnnnnng gtyggagcta gaaatagcaa gtcaacctaa ggctagtccg       60

ttatcaactt gaaaaagtgg caccgagtcg gtgcttttt                              99


<210>  27
<211>  99
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (81)..(99)
<223>  n is a, c, g, or t

<400>  27
aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct taggttgact       60

tgctatttct agctccracc nnnnnnnnnn nnnnnnnnn                              99


<210>  28
<211>  99
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (1)..(19)
<223>  n is a, c, g, or u

<400>  28
nnnnnnnnnn nnnnnnnnng guyggagcua gaaauagcaa gucaaccuaa ggcuaguccg       60

uuaucaacuu gaaaaagugg caccgagucg gugcuuuuu                              99


<210>  29
<211>  82
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  29
ctagtyggag ctagaaatag caagtcaacc taaggctagt ccgttatcaa cttgaaaaag       60

tggcaccgag tcggtgcttt tt                                                82


<210>  30
<211>  82
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  30
aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct taggttgact       60

tgctatttct agctccract ag                                                82


<210>  31
<211>  99
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (1)..(18)
<223>  n is a, c, g, or t

<400>  31
nnnnnnnnnn nnnnnnnnta gtyggagcta gaaatagcaa gtcaacctaa ggctagtccg       60

ttatcaactt gaaaaagtgg caccgagtcg gtgcttttt                              99


<210>  32
<211>  99
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (82)..(99)
<223>  n is a, c, g, or t

<400>  32
aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct taggttgact       60

tgctatttct agctccract annnnnnnnn nnnnnnnnn                              99


<210>  33
<211>  99
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (1)..(18)
<223>  n is a, c, g, or u

<400>  33
nnnnnnnnnn nnnnnnnnua guyggagcua gaaauagcaa gucaaccuaa ggcuaguccg       60

uuaucaacuu gaaaaagugg caccgagucg gugcuuuuu                              99


<210>  34
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  34
ctcggcgagc tagaaatagc aagtgccgat aaggctagtc cgttatcaac ttgaaaaagt       60

ggcaccgagt cggtgctttt t                                                 81


<210>  35
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  35
aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct tatcggcact       60

tgctatttct agctcgccga g                                                 81


<210>  36
<211>  101
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  n is a, c, g, or t

<400>  36
nnnnnnnnnn nnnnnnnnnn ctcggcgagc tagaaatagc aagtgccgat aaggctagtc       60

cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt t                          101


<210>  37
<211>  101
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (82)..(101)
<223>  n is a, c, g, or t

<400>  37
aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct tatcggcact       60

tgctatttct agctcgccga gnnnnnnnnn nnnnnnnnnn n                          101


<210>  38
<211>  101
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  n is a, c, g, or u

<400>  38
nnnnnnnnnn nnnnnnnnnn cucggcgagc uagaaauagc aagugccgau aaggcuaguc       60

cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu u                          101


<210>  39
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  39
gctcggcagc tagaaatagc aagtccgagt aaggctagtc cgttatcaac ttgaaaaagt       60

ggcaccgagt cggtgctttt t                                                 81


<210>  40
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  40
aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct tactcggact       60

tgctatttct agctgccgag c                                                 81


<210>  41
<211>  100
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (1)..(19)
<223>  n is a, c, g, or t

<400>  41
nnnnnnnnnn nnnnnnnnng ctcggcagct agaaatagca agtccgagta aggctagtcc       60

gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt                            100


<210>  42
<211>  100
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (82)..(100)
<223>  n is a, c, g, or t

<400>  42
aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct tactcggact       60

tgctatttct agctgccgag cnnnnnnnnn nnnnnnnnnn                            100


<210>  43
<211>  100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (1)..(19)
<223>  n is a, c, g, or u

<400>  43
nnnnnnnnnn nnnnnnnnng cucggcagcu agaaauagca aguccgagua aggcuagucc       60

guuaucaacu ugaaaaagug gcaccgaguc ggugcuuuuu                            100


<210>  44
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  44
gtctcggcgc tagaaatagc aagtcgagat aaggctagtc cgttatcaac ttgaaaaagt       60

ggcaccgagt cggtgctttt t                                                 81


<210>  45
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  45
aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct tatctcgact       60

tgctatttct agcgccgaga c                                                 81


<210>  46
<211>  99
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (1)..(18)
<223>  n is a, c, g, or t

<400>  46
nnnnnnnnnn nnnnnnnngt ctcggcgcta gaaatagcaa gtcgagataa ggctagtccg       60

ttatcaactt gaaaaagtgg caccgagtcg gtgcttttt                              99


<210>  47
<211>  99
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (82)..(99)
<223>  n is a, c, g, or t

<400>  47
aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct tatctcgact       60

tgctatttct agcgccgaga cnnnnnnnnn nnnnnnnnn                              99


<210>  48
<211>  99
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (1)..(18)
<223>  n is a, c, g, or u

<400>  48
nnnnnnnnnn nnnnnnnngu cucggcgcua gaaauagcaa gucgagauaa ggcuaguccg       60

uuaucaacuu gaaaaagugg caccgagucg gugcuuuuu                              99


<210>  49
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  49
gtcgatgagc tagaaatagc aagtatcgat aaggctagtc cgttatcaac ttgaaaaagt       60

ggcaccgagt cggtgctttt t                                                 81


<210>  50
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  50
aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct tatcgatact       60

tgctatttct agctcatcga c                                                 81


<210>  51
<211>  101
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  n is a, c, g, or t

<400>  51
nnnnnnnnnn nnnnnnnnnn gtcgatgagc tagaaatagc aagtatcgat aaggctagtc       60

cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt t                          101


<210>  52
<211>  101
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (82)..(101)
<223>  n is a, c, g, or t

<400>  52
aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct tatcgatact       60

tgctatttct agctcatcga cnnnnnnnnn nnnnnnnnnn n                          101


<210>  53
<211>  101
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  n is a, c, g, or u

<400>  53
nnnnnnnnnn nnnnnnnnnn gucgaugagc uagaaauagc aaguaucgau aaggcuaguc       60

cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu u                          101


<210>  54
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (28)..(28)
<223>  n is a, c, g, or t

<400>  54
gtncggcagc tagaaatagc aaggccgnat aaggctagtc cgttatcaac ttgaaaaagt       60

ggcaccgagt cggtgctttt t                                                 81


<210>  55
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (54)..(54)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (79)..(79)
<223>  n is a, c, g, or t

<400>  55
aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct tatncggcct       60

tgctatttct agctgccgna c                                                 81


<210>  56
<211>  101
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (23)..(23)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (48)..(48)
<223>  n is a, c, g, or t

<400>  56
nnnnnnnnnn nnnnnnnnnn gtncggcagc tagaaatagc aaggccgnat aaggctagtc       60

cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt t                          101


<210>  57
<211>  101
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (54)..(54)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (79)..(79)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (82)..(101)
<223>  n is a, c, g, or t

<400>  57
aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct tatncggcct       60

tgctatttct agctgccgna cnnnnnnnnn nnnnnnnnnn n                          101


<210>  58
<211>  101
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (23)..(23)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (48)..(48)
<223>  n is a, c, g, or u

<400>  58
nnnnnnnnnn nnnnnnnnnn guncggcagc uagaaauagc aaggccgnau aaggcuaguc       60

cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu u                          101


<210>  59
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  59
ctcctcgagc tagaaatagc aagtgaggat aaggctagtc cgttatcaac ttgaaaaagt       60

ggcaccgagt cggtgctttt t                                                 81


<210>  60
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  60
aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct tatcctcact       60

tgctatttct agctcgagga g                                                 81


<210>  61
<211>  101
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  n is a, c, g, or t

<400>  61
nnnnnnnnnn nnnnnnnnnn ctcctcgagc tagaaatagc aagtgaggat aaggctagtc       60

cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt t                          101


<210>  62
<211>  101
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (82)..(101)
<223>  n is a, c, g, or t

<400>  62
aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct tatcctcact       60

tgctatttct agctcgagga gnnnnnnnnn nnnnnnnnnn n                          101


<210>  63
<211>  101
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  n is a, c, g, or u

<400>  63
nnnnnnnnnn nnnnnnnnnn cuccucgagc uagaaauagc aagugaggau aaggcuaguc       60

cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu u                          101


<210>  64
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  64
cttccycagc tagaaatagc aaggrggaat aaggctagtc cgttatcaac ttgaaaaagt       60

ggcaccgagt cggtgctttt t                                                 81


<210>  65
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  65
aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct tattccycct       60

tgctatttct agctgrggaa g                                                 81


<210>  66
<211>  101
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  n is a, c, g, or t

<400>  66
nnnnnnnnnn nnnnnnnnnn cttccycagc tagaaatagc aaggrggaat aaggctagtc       60

cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt t                          101


<210>  67
<211>  101
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (82)..(101)
<223>  n is a, c, g, or t

<400>  67
aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct tattccycct       60

tgctatttct agctgrggaa gnnnnnnnnn nnnnnnnnnn n                          101


<210>  68
<211>  101
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  n is a, c, g, or u

<400>  68
nnnnnnnnnn nnnnnnnnnn cuuccycagc uagaaauagc aaggrggaau aaggcuaguc       60

cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu u                          101


<210>  69
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  69
ctccgcgagc tagaaatagc aagtgcggat aaggctagtc cgttatcaac ttgaaaaagt       60

ggcaccgagt cggtgctttt t                                                 81


<210>  70
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  70
aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct tatccgcact       60

tgctatttct agctcgcgga g                                                 81


<210>  71
<211>  101
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  n is a, c, g, or t

<400>  71
nnnnnnnnnn nnnnnnnnnn ctccgcgagc tagaaatagc aagtgcggat aaggctagtc       60

cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt t                          101


<210>  72
<211>  101
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (82)..(101)
<223>  n is a, c, g, or t

<400>  72
aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct tatccgcact       60

tgctatttct agctcgcgga gnnnnnnnnn nnnnnnnnnn n                          101


<210>  73
<211>  101
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  n is a, c, g, or u

<400>  73
nnnnnnnnnn nnnnnnnnnn cuccgcgagc uagaaauagc aagugcggau aaggcuaguc       60

cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu u                          101


<210>  74
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  74
ctccttgagc tagaaatagc aagtaaggat aaggctagtc cgttatcaac ttgaaaaagt       60

ggcaccgagt cggtgctttt t                                                 81


<210>  75
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  75
aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct tatccttact       60

tgctatttct agctcaagga g                                                 81


<210>  76
<211>  101
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  n is a, c, g, or t

<400>  76
nnnnnnnnnn nnnnnnnnnn ctccttgagc tagaaatagc aagtaaggat aaggctagtc       60

cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt t                          101


<210>  77
<211>  101
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (82)..(101)
<223>  n is a, c, g, or t

<400>  77
aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct tatccttact       60

tgctatttct agctcaagga gnnnnnnnnn nnnnnnnnnn n                          101


<210>  78
<211>  101
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  n is a, c, g, or u

<400>  78
nnnnnnnnnn nnnnnnnnnn cuccuugagc uagaaauagc aaguaaggau aaggcuaguc       60

cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu u                          101


<210>  79
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (28)..(28)
<223>  n is a, c, g, or t

<400>  79
gtncttgagc tagaaatagc aagcaagnat aaggctagtc cgttatcaac ttgaaaaagt       60

ggcaccgagt cggtgctttt t                                                 81


<210>  80
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (54)..(54)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (79)..(79)
<223>  n is a, c, g, or t

<400>  80
aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct tatncttgct       60

tgctatttct agctcaagna c                                                 81


<210>  81
<211>  101
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (23)..(23)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (48)..(48)
<223>  n is a, c, g, or t

<400>  81
nnnnnnnnnn nnnnnnnnnn gtncttgagc tagaaatagc aagcaagnat aaggctagtc       60

cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt t                          101


<210>  82
<211>  101
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (54)..(54)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (79)..(79)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (82)..(101)
<223>  n is a, c, g, or t

<400>  82
aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct tatncttgct       60

tgctatttct agctcaagna cnnnnnnnnn nnnnnnnnnn n                          101


<210>  83
<211>  101
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (23)..(23)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (48)..(48)
<223>  n is a, c, g, or u

<400>  83
nnnnnnnnnn nnnnnnnnnn guncuugagc uagaaauagc aagcaagnau aaggcuaguc       60

cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu u                          101


<210>  84
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  84
gtcgtagagc tagaaatagc aagttacgat aaggctagtc cgttatcaac ttgaaaaagt       60

ggcaccgagt cggtgctttt t                                                 81


<210>  85
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  85
aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct tatcgtaact       60

tgctatttct agctctacga c                                                 81


<210>  86
<211>  101
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  n is a, c, g, or t

<400>  86
nnnnnnnnnn nnnnnnnnnn gtcgtagagc tagaaatagc aagttacgat aaggctagtc       60

cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt t                          101


<210>  87
<211>  101
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (82)..(101)
<223>  n is a, c, g, or t

<400>  87
aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct tatcgtaact       60

tgctatttct agctctacga cnnnnnnnnn nnnnnnnnnn n                          101


<210>  88
<211>  101
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  n is a, c, g, or u

<400>  88
nnnnnnnnnn nnnnnnnnnn gucguagagc uagaaauagc aaguuacgau aaggcuaguc       60

cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu u                          101


<210>  89
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  89
gtggtcgagc tagaaatagc aagtgaccat aaggctagtc cgttatcaac ttgaaaaagt       60

ggcaccgagt cggtgctttt t                                                 81


<210>  90
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  90
aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct tatggtcact       60

tgctatttct agctcgacca c                                                 81


<210>  91
<211>  101
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  n is a, c, g, or t

<400>  91
nnnnnnnnnn nnnnnnnnnn gtggtcgagc tagaaatagc aagtgaccat aaggctagtc       60

cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt t                          101


<210>  92
<211>  101
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (82)..(101)
<223>  n is a, c, g, or t

<400>  92
aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct tatggtcact       60

tgctatttct agctcgacca cnnnnnnnnn nnnnnnnnnn n                          101


<210>  93
<211>  101
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  n is a, c, g, or u

<400>  93
nnnnnnnnnn nnnnnnnnnn guggucgagc uagaaauagc aagugaccau aaggcuaguc       60

cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu u                          101


<210>  94
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  94
gtcaaygagc tagaaatagc aagcrttgat aaggctagtc cgttatcaac ttgaaaaagt       60

ggcaccgagt cggtgctttt t                                                 81


<210>  95
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  95
aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct tatcaaygct       60

tgctatttct agctcrttga c                                                 81


<210>  96
<211>  101
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  n is a, c, g, or t

<400>  96
nnnnnnnnnn nnnnnnnnnn gtcaaygagc tagaaatagc aagcrttgat aaggctagtc       60

cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt t                          101


<210>  97
<211>  101
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (82)..(101)
<223>  n is a, c, g, or t

<400>  97
aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct tatcaaygct       60

tgctatttct agctcrttga cnnnnnnnnn nnnnnnnnnn n                          101


<210>  98
<211>  101
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  n is a, c, g, or u

<400>  98
nnnnnnnnnn nnnnnnnnnn gucaaygagc uagaaauagc aagcruugau aaggcuaguc       60

cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu u                          101


<210>  99
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  99
ctycttgagc tagaaatagc aagcaagrat aaggctagtc cgttatcaac ttgaaaaagt       60

ggcaccgagt cggtgctttt t                                                 81


<210>  100
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  100
aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct tatycttgct       60

tgctatttct agctcaagra g                                                 81


<210>  101
<211>  101
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  n is a, c, g, or t

<400>  101
nnnnnnnnnn nnnnnnnnnn ctycttgagc tagaaatagc aagcaagrat aaggctagtc       60

cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt t                          101


<210>  102
<211>  101
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (82)..(101)
<223>  n is a, c, g, or t

<400>  102
aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct tatycttgct       60

tgctatttct agctcaagra gnnnnnnnnn nnnnnnnnnn n                          101


<210>  103
<211>  101
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  n is a, c, g, or u

<400>  103
nnnnnnnnnn nnnnnnnnnn curcuugagc uagaaauagc aagcaagrau aaggcuaguc       60

cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu u                          101


<210>  104
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  104
gtgatggagc tagaaatagc aagtcatcat aaggctagtc cgttatcaac ttgaaaaagt       60

ggcaccgagt cggtgctttt t                                                 81


<210>  105
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  105
aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct tatgatgact       60

tgctatttct agctccatca c                                                 81


<210>  106
<211>  100
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (1)..(19)
<223>  n is a, c, g, or t

<400>  106
nnnnnnnnnn nnnnnnnnng tgatggagct agaaatagca agtcatcata aggctagtcc       60

gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt                            100


<210>  107
<211>  100
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (82)..(100)
<223>  n is a, c, g, or t

<400>  107
aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct tatgatgact       60

tgctatttct agctccatca cnnnnnnnnn nnnnnnnnnn                            100


<210>  108
<211>  100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (1)..(19)
<223>  n is a, c, g, or u

<400>  108
nnnnnnnnnn nnnnnnnnng ugauggagcu agaaauagca agucaucaua aggcuagucc       60

guuaucaacu ugaaaaagug gcaccgaguc ggugcuuuuu                            100


<210>  109
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  109
ctgatggagc tagaaatagc aagtcatcat aaggctagtc cgttatcaac ttgaaaaagt       60

ggcaccgagt cggtgctttt t                                                 81


<210>  110
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  110
aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct tatgatgact       60

tgctatttct agctccatca g                                                 81


<210>  111
<211>  102
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (1)..(21)
<223>  n is a, c, g, or t

<400>  111
nnnnnnnnnn nnnnnnnnnn nctgatggag ctagaaatag caagtcatca taaggctagt       60

ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt tt                         102


<210>  112
<211>  102
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (82)..(102)
<223>  n is a, c, g, or t

<400>  112
aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct tatgatgact       60

tgctatttct agctccatca gnnnnnnnnn nnnnnnnnnn nn                         102


<210>  113
<211>  102
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (1)..(21)
<223>  n is a, c, g, or u

<400>  113
nnnnnnnnnn nnnnnnnnnn ncugauggag cuagaaauag caagucauca uaaggcuagu       60

ccguuaucaa cuugaaaaag uggcaccgag ucggugcuuu uu                         102


<210>  114
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  114
ytgggcgagc tagaaatagc aagcgcccat aaggctagtc cgttatcaac ttgaaaaagt       60

ggcaccgagt cggtgctttt t                                                 81


<210>  115
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  115
aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct tatgggcgct       60

tgctatttct agctcgccca r                                                 81


<210>  116
<211>  101
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  n is a, c, g, or t

<400>  116
nnnnnnnnnn nnnnnnnnnn ytgggcgagc tagaaatagc aagcgcccat aaggctagtc       60

cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt t                          101


<210>  117
<211>  101
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (82)..(101)
<223>  n is a, c, g, or t

<400>  117
aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct tatgggcgct       60

tgctatttct agctcgccca rnnnnnnnnn nnnnnnnnnn n                          101


<210>  118
<211>  101
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  n is a, c, g, or u

<400>  118
nnnnnnnnnn nnnnnnnnnn yugggcgagc uagaaauagc aagcgcccau aaggcuaguc       60

cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu u                          101


<210>  119
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  119
ctgrgggagc tagaaatagc aagtccycat aaggctagtc cgttatcaac ttgaaaaagt       60

ggcaccgagt cggtgctttt t                                                 81


<210>  120
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  120
aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct tatgrggact       60

tgctatttct agctcccyca g                                                 81


<210>  121
<211>  102
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (1)..(21)
<223>  n is a, c, g, or t

<400>  121
nnnnnnnnnn nnnnnnnnnn nctgrgggag ctagaaatag caagtccyca taaggctagt       60

ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt tt                         102


<210>  122
<211>  102
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (82)..(102)
<223>  n is a, c, g, or t

<400>  122
aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct tatgrggact       60

tgctatttct agctcccyca gnnnnnnnnn nnnnnnnnnn nn                         102


<210>  123
<211>  101
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (1)..(21)
<223>  n is a, c, g, or u

<400>  123
nnnnnnnnnn nnnnnnnnnn ncugrgggag cuagaaauag caagucccau aaggcuaguc       60

cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu u                          101


<210>  124
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  124
gtcgatgagc tagaaatagc aagcatcgat aaggctagtc cgttatcaac ttgaaaaagt       60

ggcaccgagt cggtgctttt t                                                 81


<210>  125
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  125
aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct tatcgatgct       60

tgctatttct agctcatcga c                                                 81


<210>  126
<211>  101
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  n is a, c, g, or t

<400>  126
nnnnnnnnnn nnnnnnnnnn gtcgatgagc tagaaatagc aagcatcgat aaggctagtc       60

cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt t                          101


<210>  127
<211>  101
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (82)..(101)
<223>  n is a, c, g, or t

<400>  127
aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct tatcgatgct       60

tgctatttct agctcatcga cnnnnnnnnn nnnnnnnnnn n                          101


<210>  128
<211>  101
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (82)..(101)
<223>  n is a, c, g, or u

<400>  128
gucgaugagc uagaaauagc aagcaucgau aaggcuaguc cguuaucaac uugaaaaagu       60

ggcaccgagu cggugcuuuu unnnnnnnnn nnnnnnnnnn n                          101


<210>  129
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  129
gtccycgagc tagaaatagc aagcgrggat aaggctagtc cgttatcaac ttgaaaaagt       60

ggcaccgagt cggtgctttt t                                                 81


<210>  130
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  130
aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct tatccycgct       60

tgctatttct agctcgrgga c                                                 81


<210>  131
<211>  101
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  n is a, c, g, or t

<400>  131
nnnnnnnnnn nnnnnnnnnn gtccycgagc tagaaatagc aagcgrggat aaggctagtc       60

cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt t                          101


<210>  132
<211>  101
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (82)..(101)
<223>  n is a, c, g, or t

<400>  132
aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct tatccycgct       60

tgctatttct agctcgrgga cnnnnnnnnn nnnnnnnnnn n                          101


<210>  133
<211>  101
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  n is a, c, g, or u

<400>  133
nnnnnnnnnn nnnnnnnnnn guccycgagc uagaaauagc aagcgrggau aaggcuaguc       60

cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu u                          101


<210>  134
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  134
ctyctggagc tagaaatagc aagtcagrat aaggctagtc cgttatcaac ttgaaaaagt       60

ggcaccgagt cggtgctttt t                                                 81


<210>  135
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  135
aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct tatyctgact       60

tgctatttct agctccagra g                                                 81


<210>  136
<211>  102
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (1)..(21)
<223>  n is a, c, g, or t

<400>  136
nnnnnnnnnn nnnnnnnnnn nctyctggag ctagaaatag caagtcagra taaggctagt       60

ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt tt                         102


<210>  137
<211>  102
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (82)..(102)
<223>  n is a, c, g, or t

<400>  137
aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct tatyctgact       60

tgctatttct agctccagra gnnnnnnnnn nnnnnnnnnn nn                         102


<210>  138
<211>  101
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (1)..(21)
<223>  n is a, c, g, or u

<400>  138
nnnnnnnnnn nnnnnnnnnn ncuycuggag cuagaaauag caagucagau aaggcuaguc       60

cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu u                          101


<210>  139
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  139
ctyccgcagc tagaaatagc aaggcggrat aaggctagtc cgttatcaac ttgaaaaagt       60

ggcaccgagt cggtgctttt t                                                 81


<210>  140
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  140
aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct tatyccgcct       60

tgctatttct agctgcggra g                                                 81


<210>  141
<211>  101
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  n is a, c, g, or t

<400>  141
nnnnnnnnnn nnnnnnnnnn ctyccgcagc tagaaatagc aaggcggrat aaggctagtc       60

cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt t                          101


<210>  142
<211>  101
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (82)..(101)
<223>  n is a, c, g, or t

<400>  142
aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct tatyccgcct       60

tgctatttct agctgcggra gnnnnnnnnn nnnnnnnnnn n                          101


<210>  143
<211>  101
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  n is a, c, g, or u

<400>  143
nnnnnnnnnn nnnnnnnnnn cuyccgcagc uagaaauagc aaggcggrau aaggcuaguc       60

cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu u                          101


<210>  144
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  144
gtgttggagc tagaaatagc aagtcaacat aaggctagtc cgttatcaac ttgaaaaagt       60

ggcaccgagt cggtgctttt t                                                 81


<210>  145
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  145
aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct tatgttgact       60

tgctatttct agctccaaca c                                                 81


<210>  146
<211>  102
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (1)..(21)
<223>  n is a, c, g, or t

<400>  146
nnnnnnnnnn nnnnnnnnnn ngtgttggag ctagaaatag caagtcaaca taaggctagt       60

ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt tt                         102


<210>  147
<211>  102
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (82)..(102)
<223>  n is a, c, g, or t

<400>  147
aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct tatgttgact       60

tgctatttct agctccaaca cnnnnnnnnn nnnnnnnnnn nn                         102


<210>  148
<211>  102
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (1)..(21)
<223>  n is a, c, g, or u

<400>  148
nnnnnnnnnn nnnnnnnnnn nguguuggag cuagaaauag caagucaaca uaaggcuagu       60

ccguuaucaa cuugaaaaag uggcaccgag ucggugcuuu uu                         102


<210>  149
<211>  19
<212>  DNA
<213>  Francisella novicida

<400>  149
aatttctact gttgtagat                                                    19


<210>  150
<211>  19
<212>  DNA
<213>  Francisella novicida

<400>  150
atctacaaca gtagaaatt                                                    19


<210>  151
<211>  19
<212>  RNA
<213>  Francisella novicida

<400>  151
aauuucuacu guuguagau                                                    19


<210>  152
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  152
aattgttggt gttccract                                                    19


<210>  153
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  153
agtyggaaca ccaacaatt                                                    19


<210>  154
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (20)..(38)
<223>  n is a, c, g, or t

<400>  154
aattgttggt gttccractn nnnnnnnnnn nnnnnnnn                               38


<210>  155
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (1)..(19)
<223>  n is a, c, g, or t

<400>  155
nnnnnnnnnn nnnnnnnnna gtyggaacac caacaatt                               38


<210>  156
<211>  38
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (20)..(38)
<223>  n is a, c, g, or u

<400>  156
aauuguuggu guuccracun nnnnnnnnnn nnnnnnnn                               38


<210>  157
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  157
aatttgttgt gtccaacat                                                    19


<210>  158
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  158
atgttggaca caacaaatt                                                    19


<210>  159
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (20)..(37)
<223>  n is a, c, g, or t

<400>  159
aatttgttgt gtccaacatn nnnnnnnnnn nnnnnnn                                37


<210>  160
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (1)..(18)
<223>  n is a, c, g, or t

<400>  160
nnnnnnnnnn nnnnnnnnat gttggacaca acaaatt                                37


<210>  161
<211>  37
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (20)..(37)
<223>  n is a, c, g, or u

<400>  161
aauuuguugu guccaacaun nnnnnnnnnn nnnnnnn                                37


<210>  162
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  162
aattgttggt gttccracta t                                                 21


<210>  163
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  163
atagtyggaa caccaacaat t                                                 21


<210>  164
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (22)..(40)
<223>  n is a, c, g, or t

<400>  164
aattgttggt gttccracta tnnnnnnnnn nnnnnnnnnn                             40


<210>  165
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (1)..(19)
<223>  n is a, c, g, or t

<400>  165
nnnnnnnnnn nnnnnnnnna tagtyggaac accaacaatt                             40


<210>  166
<211>  40
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (22)..(40)
<223>  n is a, c, g, or u

<400>  166
aauuguuggu guuccracua unnnnnnnnn nnnnnnnnnn                             40


<210>  167
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  167
aatttgttgt gtccaacat                                                    19


<210>  168
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  168
atgttggaca caacaaatt                                                    19


<210>  169
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (20)..(37)
<223>  n is a, c, g, or t

<400>  169
aatttgttgt gtccaacatn nnnnnnnnnn nnnnnnn                                37


<210>  170
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (1)..(18)
<223>  n is a, c, g, or t

<400>  170
nnnnnnnnnn nnnnnnnnat gttggacaca acaaatt                                37


<210>  171
<211>  37
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (20)..(37)
<223>  n is a, c, g, or u

<400>  171
aauuuguugu guccaacaun nnnnnnnnnn nnnnnnn                                37


<210>  172
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  172
aattctcggt gtgccgagt                                                    19


<210>  173
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  173
actcggcaca ccgagaatt                                                    19


<210>  174
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (20)..(38)
<223>  n is a, c, g, or t

<400>  174
aattctcggt gtgccgagtn nnnnnnnnnn nnnnnnnn                               38


<210>  175
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (1)..(19)
<223>  n is a, c, g, or t

<400>  175
nnnnnnnnnn nnnnnnnnna ctcggcacac cgagaatt                               38


<210>  176
<211>  38
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (20)..(38)
<223>  n is a, c, g, or u

<400>  176
aauucucggu gugccgagun nnnnnnnnnn nnnnnnnn                               38


<210>  177
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  177
aatttctcgt ggccgagat                                                    19


<210>  178
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  178
atctcggcca cgagaaatt                                                    19


<210>  179
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (20)..(37)
<223>  n is a, c, g, or t

<400>  179
aatttctcgt ggccgagatn nnnnnnnnnn nnnnnnn                                37


<210>  180
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (1)..(18)
<223>  n is a, c, g, or t

<400>  180
nnnnnnnnnn nnnnnnnnat ctcggccacg agaaatt                                37


<210>  181
<211>  37
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (20)..(37)
<223>  n is a, c, g, or u

<400>  181
aauuucucgu ggccgagaun nnnnnnnnnn nnnnnnn                                37


<210>  182
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  182
aattctcggt gtgccgagta t                                                 21


<210>  183
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  183
atactcggca caccgagaat t                                                 21


<210>  184
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (22)..(40)
<223>  n is a, c, g, or t

<400>  184
aattctcggt gtgccgagta tnnnnnnnnn nnnnnnnnnn                             40


<210>  185
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (1)..(19)
<223>  n is a, c, g, or t

<400>  185
nnnnnnnnnn nnnnnnnnna tactcggcac accgagaatt                             40


<210>  186
<211>  40
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (22)..(40)
<223>  n is a, c, g, or u

<400>  186
aauucucggu gugccgagua unnnnnnnnn nnnnnnnnnn                             40


<210>  187
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  187
aatttctcgt ggccgagata t                                                 21


<210>  188
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  188
atatctcggc cacgagaaat t                                                 21


<210>  189
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (22)..(39)
<223>  n is a, c, g, or t

<400>  189
aatttctcgt ggccgagata tnnnnnnnnn nnnnnnnnn                              39


<210>  190
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (1)..(18)
<223>  n is a, c, g, or t

<400>  190
nnnnnnnnnn nnnnnnnnat atctcggcca cgagaaatt                              39


<210>  191
<211>  39
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (22)..(39)
<223>  n is a, c, g, or u

<400>  191
aauuucucgu ggccgagaua unnnnnnnnn nnnnnnnnn                              39


<210>  192
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  192
aattgtcgat gtatcgact                                                    19


<210>  193
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  193
agtcgataca tcgacaatt                                                    19


<210>  194
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (20)..(38)
<223>  n is a, c, g, or t

<400>  194
aattgtcgat gtatcgactn nnnnnnnnnn nnnnnnnn                               38


<210>  195
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (1)..(19)
<223>  n is a, c, g, or t

<400>  195
nnnnnnnnnn nnnnnnnnna gtcgatacat cgacaatt                               38


<210>  196
<211>  38
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (20)..(38)
<223>  n is a, c, g, or u

<400>  196
aauugucgau guaucgacun nnnnnnnnnn nnnnnnnn                               38


<210>  197
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (16)..(16)
<223>  n is a, c, g, or t

<400>  197
aattgttcgt ggccgnact                                                    19


<210>  198
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (4)..(4)
<223>  n is a, c, g, or t

<400>  198
agtncggcca cgaacaatt                                                    19


<210>  199
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (16)..(16)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (20)..(38)
<223>  n is a, c, g, or t

<400>  199
aattgttcgt ggccgnactn nnnnnnnnnn nnnnnnnn                               38


<210>  200
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (1)..(19)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (23)..(23)
<223>  n is a, c, g, or t

<400>  200
nnnnnnnnnn nnnnnnnnna gtncggccac gaacaatt                               38


<210>  201
<211>  38
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (16)..(16)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (20)..(38)
<223>  n is a, c, g, or u

<400>  201
aauuguucgu ggccgnacun nnnnnnnnnn nnnnnnnn                               38


<210>  202
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  202
aattctcctt gtgaggagt                                                    19


<210>  203
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  203
actcctcaca aggagaatt                                                    19


<210>  204
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (20)..(38)
<223>  n is a, c, g, or t

<400>  204
aattctcctt gtgaggagtn nnnnnnnnnn nnnnnnnn                               38


<210>  205
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (1)..(19)
<223>  n is a, c, g, or t

<400>  205
nnnnnnnnnn nnnnnnnnna ctcctcacaa ggagaatt                               38


<210>  206
<211>  38
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (20)..(38)
<223>  n is a, c, g, or u

<400>  206
aauucuccuu gugaggagun nnnnnnnnnn nnnnnnnn                               38


<210>  207
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  207
aattcttcct ggrggaag                                                     18


<210>  208
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  208
cttccyccag gaagaatt                                                     18


<210>  209
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (19)..(37)
<223>  n is a, c, g, or t

<400>  209
aattcttcct ggrggaagnn nnnnnnnnnn nnnnnnn                                37


<210>  210
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (1)..(19)
<223>  n is a, c, g, or t

<400>  210
nnnnnnnnnn nnnnnnnnnc ttccyccagg aagaatt                                37


<210>  211
<211>  37
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (19)..(37)
<223>  n is a, c, g, or u

<400>  211
aauucuuccu ggrggaagnn nnnnnnnnnn nnnnnnn                                37


<210>  212
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  212
aattctccgt gtgcggagt                                                    19


<210>  213
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  213
actccgcaca cggagaatt                                                    19


<210>  214
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (20)..(38)
<223>  n is a, c, g, or t

<400>  214
aattctccgt gtgcggagtn nnnnnnnnnn nnnnnnnn                               38


<210>  215
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (1)..(19)
<223>  n is a, c, g, or t

<400>  215
nnnnnnnnnn nnnnnnnnna ctccgcacac ggagaatt                               38


<210>  216
<211>  38
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (20)..(38)
<223>  n is a, c, g, or u

<400>  216
aauucuccgu gugcggagun nnnnnnnnnn nnnnnnnn                               38


<210>  217
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  217
aattctcctt gtaaggagt                                                    19


<210>  218
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  218
actccttaca aggagaatt                                                    19


<210>  219
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (20)..(38)
<223>  n is a, c, g, or t

<400>  219
aattctcctt gtaaggagtn nnnnnnnnnn nnnnnnnn                               38


<210>  220
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (1)..(19)
<223>  n is a, c, g, or t

<400>  220
nnnnnnnnnn nnnnnnnnna ctccttacaa ggagaatt                               38


<210>  221
<211>  38
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (20)..(38)
<223>  n is a, c, g, or u

<400>  221
aauucuccuu guaaggagun nnnnnnnnnn nnnnnnnn                               38


<210>  222
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (16)..(16)
<223>  n is a, c, g, or t

<400>  222
aattgttctt gcaagnact                                                    19


<210>  223
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (4)..(4)
<223>  n is a, c, g, or t

<400>  223
agtncttgca agaacaatt                                                    19


<210>  224
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (16)..(16)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (20)..(38)
<223>  n is a, c, g, or t

<400>  224
aattgttctt gcaagnactn nnnnnnnnnn nnnnnnnn                               38


<210>  225
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (1)..(19)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (23)..(23)
<223>  n is a, c, g, or t

<400>  225
nnnnnnnnnn nnnnnnnnna gtncttgcaa gaacaatt                               38


<210>  226
<211>  38
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (16)..(16)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (20)..(38)
<223>  n is a, c, g, or u

<400>  226
aauuguucuu gcaagnacun nnnnnnnnnn nnnnnnnn                               38


<210>  227
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  227
aattgtcgtt gttacgact                                                    19


<210>  228
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  228
agtcgtaaca acgacaatt                                                    19


<210>  229
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (20)..(38)
<223>  n is a, c, g, or t

<400>  229
aattgtcgtt gttacgactn nnnnnnnnnn nnnnnnnn                               38


<210>  230
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (1)..(19)
<223>  n is a, c, g, or t

<400>  230
nnnnnnnnnn nnnnnnnnna gtcgtaacaa cgacaatt                               38


<210>  231
<211>  38
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (20)..(38)
<223>  n is a, c, g, or u

<400>  231
aauugucguu guuacgacun nnnnnnnnnn nnnnnnnn                               38


<210>  232
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  232
aattgtggtt gtgaccact                                                    19


<210>  233
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  233
agtggtcaca accacaatt                                                    19


<210>  234
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (20)..(38)
<223>  n is a, c, g, or t

<400>  234
aattgtggtt gtgaccactn nnnnnnnnnn nnnnnnnn                               38


<210>  235
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (1)..(19)
<223>  n is a, c, g, or t

<400>  235
nnnnnnnnnn nnnnnnnnna gtggtcacaa ccacaatt                               38


<210>  236
<211>  38
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (20)..(38)
<223>  n is a, c, g, or u

<400>  236
aauugugguu gugaccacun nnnnnnnnnn nnnnnnnn                               38


<210>  237
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  237
aattgtcaat gcrttgact                                                    19


<210>  238
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  238
agtcaaygca ttgacaatt                                                    19


<210>  239
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (20)..(38)
<223>  n is a, c, g, or t

<400>  239
aattgtcaat gcrttgactn nnnnnnnnnn nnnnnnnn                               38


<210>  240
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (1)..(19)
<223>  n is a, c, g, or t

<400>  240
nnnnnnnnnn nnnnnnnnna gtcaaygcat tgacaatt                               38


<210>  241
<211>  38
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (20)..(38)
<223>  n is a, c, g, or u

<400>  241
aauugucaau gcruugacun nnnnnnnnnn nnnnnnnn                               38


<210>  242
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  242
aattcttctt gcaagragt                                                    19


<210>  243
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  243
actycttgca agaagaatt                                                    19


<210>  244
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (20)..(38)
<223>  n is a, c, g, or t

<400>  244
aattcttctt gcaagragtn nnnnnnnnnn nnnnnnnn                               38


<210>  245
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (1)..(19)
<223>  n is a, c, g, or t

<400>  245
nnnnnnnnnn nnnnnnnnna ctycttgcaa gaagaatt                               38


<210>  246
<211>  38
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (20)..(38)
<223>  n is a, c, g, or u

<400>  246
aauucuucuu gcaagragun nnnnnnnnnn nnnnnnnn                               38


<210>  247
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  247
aattgtgatt gtcatcact                                                    19


<210>  248
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  248
agtgatgaca atcacaatt                                                    19


<210>  249
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (20)..(37)
<223>  n is a, c, g, or t

<400>  249
aattgtgatt gtcatcactn nnnnnnnnnn nnnnnnn                                37


<210>  250
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (1)..(18)
<223>  n is a, c, g, or t

<400>  250
nnnnnnnnnn nnnnnnnnag tgatgacaat cacaatt                                37


<210>  251
<211>  37
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (20)..(37)
<223>  n is a, c, g, or u

<400>  251
aauugugauu gucaucacun nnnnnnnnnn nnnnnnn                                37


<210>  252
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  252
aattctgatt gtcatcagt                                                    19


<210>  253
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  253
actgatgaca atcagaatt                                                    19


<210>  254
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (20)..(39)
<223>  n is a, c, g, or t

<400>  254
aattctgatt gtcatcagtn nnnnnnnnnn nnnnnnnnn                              39


<210>  255
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  n is a, c, g, or t

<400>  255
nnnnnnnnnn nnnnnnnnnn actgatgaca atcagaatt                              39


<210>  256
<211>  39
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (20)..(39)
<223>  n is a, c, g, or u

<400>  256
aauucugauu gucaucagun nnnnnnnnnn nnnnnnnnn                              39


<210>  257
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  257
aattytgggt gcgcccart                                                    19


<210>  258
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  258
aytgggcgca cccaraatt                                                    19


<210>  259
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (20)..(38)
<223>  n is a, c, g, or t

<400>  259
aattytgggt gcgcccartn nnnnnnnnnn nnnnnnnn                               38


<210>  260
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (1)..(19)
<223>  n is a, c, g, or t

<400>  260
nnnnnnnnnn nnnnnnnnna ytgggcgcac ccaraatt                               38


<210>  261
<211>  38
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (20)..(38)
<223>  n is a, c, g, or u

<400>  261
aauuyugggu gcgcccarun nnnnnnnnnn nnnnnnnn                               38


<210>  262
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  262
aattgtgggt gtccycagt                                                    19


<210>  263
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  263
actgrggaca cccacaatt                                                    19


<210>  264
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (20)..(39)
<223>  n is a, c, g, or t

<400>  264
aattgtgggt gtccycagtn nnnnnnnnnn nnnnnnnnn                              39


<210>  265
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  n is a, c, g, or t

<400>  265
nnnnnnnnnn nnnnnnnnnn actgrggaca cccacaatt                              39


<210>  266
<211>  39
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (20)..(39)
<223>  n is a, c, g, or u

<400>  266
aauugugggu guccycagun nnnnnnnnnn nnnnnnnnn                              39


<210>  267
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  267
aattgtcgat gcatcgact                                                    19


<210>  268
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  268
agtcgatgca tcgacaatt                                                    19


<210>  269
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (20)..(38)
<223>  n is a, c, g, or t

<400>  269
aattgtcgat gcatcgactn nnnnnnnnnn nnnnnnnn                               38


<210>  270
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (1)..(19)
<223>  n is a, c, g, or t

<400>  270
nnnnnnnnnn nnnnnnnnna gtcgatgcat cgacaatt                               38


<210>  271
<211>  38
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (20)..(38)
<223>  n is a, c, g, or u

<400>  271
aauugucgau gcaucgacun nnnnnnnnnn nnnnnnnn                               38


<210>  272
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  272
aattgtcctt gcgrggact                                                    19


<210>  273
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  273
agtccycgca aggacaatt                                                    19


<210>  274
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (20)..(38)
<223>  n is a, c, g, or t

<400>  274
aattgtcctt gcgrggactn nnnnnnnnnn nnnnnnnn                               38


<210>  275
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (1)..(19)
<223>  n is a, c, g, or t

<400>  275
nnnnnnnnnn nnnnnnnnna gtccycgcaa ggacaatt                               38


<210>  276
<211>  38
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (20)..(38)
<223>  n is a, c, g, or u

<400>  276
aauuguccuu gcgrggacun nnnnnnnnnn nnnnnnnn                               38


<210>  277
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  277
aattcttctt gtcagragt                                                    19


<210>  278
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  278
actyctgaca agaagaatt                                                    19


<210>  279
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (20)..(38)
<223>  n is a, c, g, or t

<400>  279
aattcttctt gtcagragtn nnnnnnnnnn nnnnnnnn                               38


<210>  280
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (1)..(19)
<223>  n is a, c, g, or t

<400>  280
nnnnnnnnnn nnnnnnnnna ctyctgacaa gaagaatt                               38


<210>  281
<211>  38
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (20)..(38)
<223>  n is a, c, g, or u

<400>  281
aauucuucuu gucagragun nnnnnnnnnn nnnnnnnn                               38


<210>  282
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  282
aattcttcct ggcggragt                                                    19


<210>  283
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  283
actyccgcca ggaagaatt                                                    19


<210>  284
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (20)..(38)
<223>  n is a, c, g, or t

<400>  284
aattcttcct ggcggragtn nnnnnnnnnn nnnnnnnn                               38


<210>  285
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (1)..(19)
<223>  n is a, c, g, or t

<400>  285
nnnnnnnnnn nnnnnnnnna ctyccgccag gaagaatt                               38


<210>  286
<211>  38
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (20)..(38)
<223>  n is a, c, g, or u

<400>  286
aauucuuccu ggcggragun nnnnnnnnnn nnnnnnnn                               38


<210>  287
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  287
aattctccct ggcgggagt                                                    19


<210>  288
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  288
actcccgcca gggagaatt                                                    19


<210>  289
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (20)..(39)
<223>  n is a, c, g, or t

<400>  289
aattctccct ggcgggagtn nnnnnnnnnn nnnnnnnnn                              39


<210>  290
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  n is a, c, g, or t

<400>  290
nnnnnnnnnn nnnnnnnnnn actcccgcca gggagaatt                              39


<210>  291
<211>  39
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (20)..(39)
<223>  n is a, c, g, or u

<400>  291
aauucucccu ggcgggagun nnnnnnnnnn nnnnnnnnn                              39


<210>  292
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  292
aatttgtcgt gatcgacat                                                    19


<210>  293
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  293
atgtcgatca cgacaaatt                                                    19


<210>  294
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (20)..(37)
<223>  n is a, c, g, or t

<400>  294
aatttgtcgt gatcgacatn nnnnnnnnnn nnnnnnn                                37


<210>  295
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (1)..(18)
<223>  n is a, c, g, or t

<400>  295
nnnnnnnnnn nnnnnnnnat gtcgatcacg acaaatt                                37


<210>  296
<211>  37
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (20)..(37)
<223>  n is a, c, g, or u

<400>  296
aauuugucgu gaucgacaun nnnnnnnnnn nnnnnnn                                37


<210>  297
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (15)..(15)
<223>  n is a, c, g, or t

<400>  297
aatttgttct gccgnacat                                                    19


<210>  298
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  n is a, c, g, or t

<400>  298
atgtncggca gaacaaatt                                                    19


<210>  299
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (15)..(15)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (20)..(37)
<223>  n is a, c, g, or t

<400>  299
aatttgttct gccgnacatn nnnnnnnnnn nnnnnnn                                37


<210>  300
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (1)..(18)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (23)..(23)
<223>  n is a, c, g, or t

<400>  300
nnnnnnnnnn nnnnnnnnat gtncggcaga acaaatt                                37


<210>  301
<211>  37
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (15)..(15)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (20)..(37)
<223>  n is a, c, g, or u

<400>  301
aauuuguucu gccgnacaun nnnnnnnnnn nnnnnnn                                37


<210>  302
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  302
aatttctcct ggaggagat                                                    19


<210>  303
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  303
atctcctcca ggagaaatt                                                    19


<210>  304
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (20)..(37)
<223>  n is a, c, g, or t

<400>  304
aatttctcct ggaggagatn nnnnnnnnnn nnnnnnn                                37


<210>  305
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (1)..(18)
<223>  n is a, c, g, or t

<400>  305
nnnnnnnnnn nnnnnnnnat ctcctccagg agaaatt                                37


<210>  306
<211>  37
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (20)..(37)
<223>  n is a, c, g, or u

<400>  306
aauuucuccu ggaggagaun nnnnnnnnnn nnnnnnn                                37


<210>  307
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  307
aatttcttct grggaagat                                                    19


<210>  308
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  308
atcttccyca gaagaaatt                                                    19


<210>  309
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (20)..(37)
<223>  n is a, c, g, or t

<400>  309
aatttcttct grggaagatn nnnnnnnnnn nnnnnnn                                37


<210>  310
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (1)..(18)
<223>  n is a, c, g, or t

<400>  310
nnnnnnnnnn nnnnnnnnat cttccycaga agaaatt                                37


<210>  311
<211>  37
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (20)..(37)
<223>  n is a, c, g, or u

<400>  311
aauuucuucu grggaagaun nnnnnnnnnn nnnnnnn                                37


<210>  312
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  312
aatttctcct ggcggagat                                                    19


<210>  313
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  313
atctccgcca ggagaaatt                                                    19


<210>  314
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (20)..(37)
<223>  n is a, c, g, or t

<400>  314
aatttctcct ggcggagatn nnnnnnnnnn nnnnnnn                                37


<210>  315
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (1)..(18)
<223>  n is a, c, g, or t

<400>  315
nnnnnnnnnn nnnnnnnnat ctccgccagg agaaatt                                37


<210>  316
<211>  37
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (20)..(37)
<223>  n is a, c, g, or u

<400>  316
aauuucuccu ggcggagaun nnnnnnnnnn nnnnnnn                                37


<210>  317
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  317
aatttctcct gaaggagat                                                    19


<210>  318
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  318
atctccttca ggagaaatt                                                    19


<210>  319
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (20)..(37)
<223>  n is a, c, g, or t

<400>  319
aatttctcct gaaggagatn nnnnnnnnnn nnnnnnn                                37


<210>  320
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (1)..(18)
<223>  n is a, c, g, or t

<400>  320
nnnnnnnnnn nnnnnnnnat ctccttcagg agaaatt                                37


<210>  321
<211>  37
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (20)..(37)
<223>  n is a, c, g, or u

<400>  321
aauuucuccu gaaggagaun nnnnnnnnnn nnnnnnn                                37


<210>  322
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (15)..(15)
<223>  n is a, c, g, or t

<400>  322
aatttgttct caagnacat                                                    19


<210>  323
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  n is a, c, g, or t

<400>  323
atgtncttga gaacaaatt                                                    19


<210>  324
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (15)..(15)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (20)..(37)
<223>  n is a, c, g, or t

<400>  324
aatttgttct caagnacatn nnnnnnnnnn nnnnnnn                                37


<210>  325
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (1)..(18)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (23)..(23)
<223>  n is a, c, g, or t

<400>  325
nnnnnnnnnn nnnnnnnnat gtncttgaga acaaatt                                37


<210>  326
<211>  37
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (15)..(15)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (20)..(37)
<223>  n is a, c, g, or u

<400>  326
aauuuguucu caagnacaun nnnnnnnnnn nnnnnnn                                37


<210>  327
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  327
aatttgtcgt gtacgacat                                                    19


<210>  328
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  328
atgtcgtaca cgacaaatt                                                    19


<210>  329
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (20)..(37)
<223>  n is a, c, g, or t

<400>  329
aatttgtcgt gtacgacatn nnnnnnnnnn nnnnnnn                                37


<210>  330
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (1)..(18)
<223>  n is a, c, g, or t

<400>  330
nnnnnnnnnn nnnnnnnnat gtcgtacacg acaaatt                                37


<210>  331
<211>  37
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (20)..(37)
<223>  n is a, c, g, or u

<400>  331
aauuugucgu guacgacaun nnnnnnnnnn nnnnnnn                                37


<210>  332
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  332
aatttgtggt ggaccacat                                                    19


<210>  333
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  333
atgtggtcca ccacaaatt                                                    19


<210>  334
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (20)..(37)
<223>  n is a, c, g, or t

<400>  334
aatttgtggt ggaccacatn nnnnnnnnnn nnnnnnn                                37


<210>  335
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (1)..(18)
<223>  n is a, c, g, or t

<400>  335
nnnnnnnnnn nnnnnnnnat gtggtccacc acaaatt                                37


<210>  336
<211>  37
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (20)..(37)
<223>  n is a, c, g, or u

<400>  336
aauuuguggu ggaccacaun nnnnnnnnnn nnnnnnn                                37


<210>  337
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  337
aatttgtcat crttgacat                                                    19


<210>  338
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  338
atgtcaayga tgacaaatt                                                    19


<210>  339
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (20)..(37)
<223>  n is a, c, g, or t

<400>  339
aatttgtcat crttgacatn nnnnnnnnnn nnnnnnn                                37


<210>  340
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (1)..(18)
<223>  n is a, c, g, or t

<400>  340
nnnnnnnnnn nnnnnnnnat gtcaaygatg acaaatt                                37


<210>  341
<211>  37
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (20)..(37)
<223>  n is a, c, g, or u

<400>  341
aauuugucau cruugacaun nnnnnnnnnn nnnnnnn                                37


<210>  342
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  342
aatttcttct caagragat                                                    19


<210>  343
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  343
atctycttga gaagaaatt                                                    19


<210>  344
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (20)..(37)
<223>  n is a, c, g, or t

<400>  344
aatttcttct caagragatn nnnnnnnnnn nnnnnnn                                37


<210>  345
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (1)..(18)
<223>  n is a, c, g, or t

<400>  345
nnnnnnnnnn nnnnnnnnat ctycttgaga agaaatt                                37


<210>  346
<211>  37
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (20)..(37)
<223>  n is a, c, g, or u

<400>  346
aauuucuucu caagragaun nnnnnnnnnn nnnnnnn                                37


<210>  347
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  347
aatttctgat gcatcagat                                                    19


<210>  348
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  348
atctgatgca tcagaaatt                                                    19


<210>  349
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (20)..(38)
<223>  n is a, c, g, or t

<400>  349
aatttctgat gcatcagatn nnnnnnnnnn nnnnnnnn                               38


<210>  350
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (1)..(19)
<223>  n is a, c, g, or t

<400>  350
nnnnnnnnnn nnnnnnnnna tctgatgcat cagaaatt                               38


<210>  351
<211>  38
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (20)..(38)
<223>  n is a, c, g, or u

<400>  351
aauuucugau gcaucagaun nnnnnnnnnn nnnnnnnn                               38


<210>  352
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  352
aattaytggt cgcccarat                                                    19


<210>  353
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  353
atytgggcga ccartaatt                                                    19


<210>  354
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (20)..(37)
<223>  n is a, c, g, or t

<400>  354
aattaytggt cgcccaratn nnnnnnnnnn nnnnnnn                                37


<210>  355
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (1)..(18)
<223>  n is a, c, g, or t

<400>  355
nnnnnnnnnn nnnnnnnnat ytgggcgacc artaatt                                37


<210>  356
<211>  37
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (20)..(37)
<223>  n is a, c, g, or u

<400>  356
aauuayucca gcgggaraun nnnnnnnnnn nnnnnnn                                37


<210>  357
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  357
aatttgtgrt gccycacat                                                    19


<210>  358
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  358
atgtgrggca ycacaaatt                                                    19


<210>  359
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (20)..(38)
<223>  n is a, c, g, or t

<400>  359
aatttgtgrt gccycacatn nnnnnnnnnn nnnnnnnn                               38


<210>  360
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (1)..(19)
<223>  n is a, c, g, or t

<400>  360
nnnnnnnnnn nnnnnnnnna tgtgrggcay cacaaatt                               38


<210>  361
<211>  38
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (20)..(38)
<223>  n is a, c, g, or u

<400>  361
aauuugugru gccycacaun nnnnnnnnnn nnnnnnnn                               38


<210>  362
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  362
aatttgtcgt catcgacat                                                    19


<210>  363
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  363
atgtcgatga cgacaaatt                                                    19


<210>  364
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (20)..(37)
<223>  n is a, c, g, or t

<400>  364
aatttgtcgt catcgacatn nnnnnnnnnn nnnnnnn                                37


<210>  365
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (1)..(18)
<223>  n is a, c, g, or t

<400>  365
nnnnnnnnnn nnnnnnnnat gtcgatgacg acaaatt                                37


<210>  366
<211>  37
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (20)..(37)
<223>  n is a, c, g, or u

<400>  366
aauuugucgu caucgacaun nnnnnnnnnn nnnnnnn                                37


<210>  367
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  367
aatttgtcct cgrggacat                                                    19


<210>  368
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  368
atgtccycga ggacaaatt                                                    19


<210>  369
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (20)..(37)
<223>  n is a, c, g, or t

<400>  369
aatttgtcct cgrggacatn nnnnnnnnnn nnnnnnn                                37


<210>  370
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (1)..(18)
<223>  n is a, c, g, or t

<400>  370
nnnnnnnnnn nnnnnnnnat gtccycgagg acaaatt                                37


<210>  371
<211>  37
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (20)..(37)
<223>  n is a, c, g, or u

<400>  371
aauuuguccu cgrggacaun nnnnnnnnnn nnnnnnn                                37


<210>  372
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  372
aatttcttct gcagragat                                                    19


<210>  373
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  373
atctyctgca gaagaaatt                                                    19


<210>  374
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (20)..(38)
<223>  n is a, c, g, or t

<400>  374
aatttcttct gcagragatn nnnnnnnnnn nnnnnnnn                               38


<210>  375
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (1)..(19)
<223>  n is a, c, g, or t

<400>  375
nnnnnnnnnn nnnnnnnnna tctyctgcag aagaaatt                               38


<210>  376
<211>  38
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (20)..(38)
<223>  n is a, c, g, or u

<400>  376
aauuucuucu gcagragaun nnnnnnnnnn nnnnnnnn                               38


<210>  377
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  377
aatttcttct gcggragat                                                    19


<210>  378
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  378
atctyccgca gaagaaatt                                                    19


<210>  379
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (20)..(37)
<223>  n is a, c, g, or t

<400>  379
aatttcttct gcggragatn nnnnnnnnnn nnnnnnn                                37


<210>  380
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (1)..(18)
<223>  n is a, c, g, or t

<400>  380
nnnnnnnnnn nnnnnnnnat ctyccgcaga agaaatt                                37


<210>  381
<211>  37
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (20)..(37)
<223>  n is a, c, g, or u

<400>  381
aauuucuucu gcggragaun nnnnnnnnnn nnnnnnn                                37


<210>  382
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  382
aatttgtgtt gcaacacat                                                    19


<210>  383
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  383
atgtgttgca acacaaatt                                                    19


<210>  384
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (20)..(38)
<223>  n is a, c, g, or t

<400>  384
aatttgtgtt gcaacacatn nnnnnnnnnn nnnnnnnn                               38


<210>  385
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (1)..(19)
<223>  n is a, c, g, or t

<400>  385
nnnnnnnnnn nnnnnnnnna tgtgttgcaa cacaaatt                               38


<210>  386
<211>  38
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (20)..(38)
<223>  n is a, c, g, or u

<400>  386
aauuuguguu gcaacacaun nnnnnnnnnn nnnnnnnn                               38


<210>  387
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  387
aatttcctgt gcatcaggat                                                   20


<210>  388
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  388
atcctgatgc acaggaaatt                                                   20


<210>  389
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (21)..(38)
<223>  n is a, c, g, or t

<400>  389
aatttcctgt gcatcaggat nnnnnnnnnn nnnnnnnn                               38


<210>  390
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (1)..(18)
<223>  n is a, c, g, or t

<400>  390
nnnnnnnnnn nnnnnnnnat cctgatgcac aggaaatt                               38


<210>  391
<211>  38
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (21)..(38)
<223>  n is a, c, g, or u

<400>  391
aauuuccugu gcaucaggau nnnnnnnnnn nnnnnnnn                               38


<210>  392
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  392
aatttcctgt gccycaggat                                                   20


<210>  393
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  393
atcctgrggc acaggaaatt                                                   20


<210>  394
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (21)..(38)
<223>  n is a, c, g, or t

<400>  394
aatttcctgt gccycaggat nnnnnnnnnn nnnnnnnn                               38


<210>  395
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (1)..(18)
<223>  n is a, c, g, or t

<400>  395
nnnnnnnnnn nnnnnnnnat cctgrggcac aggaaatt                               38


<210>  396
<211>  38
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (21)..(38)
<223>  n is a, c, g, or u

<400>  396
aauuuccugu gccycaggau nnnnnnnnnn nnnnnnnn                               38


<210>  397
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  397
aatttccttt gcagraggat                                                   20


<210>  398
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  398
atcctyctgc aaaggaaatt                                                   20


<210>  399
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (21)..(38)
<223>  n is a, c, g, or t

<400>  399
aatttccttt gcagraggat nnnnnnnnnn nnnnnnnn                               38


<210>  400
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (1)..(18)
<223>  n is a, c, g, or t

<400>  400
nnnnnnnnnn nnnnnnnnat cctyctgcaa aggaaatt                               38


<210>  401
<211>  38
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (21)..(38)
<223>  n is a, c, g, or u

<400>  401
aauuuccuuu gcagraggau nnnnnnnnnn nnnnnnnn                               38


<210>  402
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  402
aatttcgtgt gcaacacgat                                                   20


<210>  403
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.

<400>  403
atcgtgttgc acacgaaatt                                                   20


<210>  404
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (21)..(38)
<223>  n is a, c, g, or t

<400>  404
aatttcgtgt gcaacacgat nnnnnnnnnn nnnnnnnn                               38


<210>  405
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (1)..(18)
<223>  n is a, c, g, or t

<400>  405
nnnnnnnnnn nnnnnnnnat cgtgttgcac acgaaatt                               38


<210>  406
<211>  38
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding constant region of sgRNA/crRNA with 
       non-palindromic recognition site for restriction enzyme (sense or
       antisense), polynucleotide encoding for constant and variable 
       regions of sgRNA/crRNA (sense or antisense), or sgRNA/crRNA.


<220>
<221>  misc_feature
<222>  (21)..(38)
<223>  n is a, c, g, or u

<400>  406
aauuucgugu gcaacacgau nnnnnnnnnn nnnnnnnn                               38


<210>  407
<211>  1653
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide containing EGFP Transgene sequence

<400>  407
agctcggatc caaaagctta aatcagttgt gttaaataag agacattcaa aataaatgta       60

aatgagctct ccaaatcagc agacttaaca ttctttaaaa tgattgattc aatagtgata      120

aaaatcaggc atagccagtt gtaactttag ataaattaca gaaaatgtca aatacagaga      180

accgattctt ttttatgata catccaagca cacatttaac acaatccagg caaaccccga      240

atttcacagt cacaagcact gtttgtacaa gagctttgcc taaggacaca cagtctctat      300

aagtccaggt cgttggtttc actcttattt taaacatgtg acatttttcc tgccatcctg      360

tcttaggctg ctgtttgctt cattccatgt cacattaaat tcctcagtag caccttttac      420

acacacagcc aatcttttcc agaaaattca attgctttga agagataatg tgtgaacaaa      480

tccatttaga aaaggaaaat taagaatttg taaaatcatc tgtaaattgt tggcattctt      540

ctgtatatga acatcacatc atttacaggt aaaggtctgg tcattaatta tatgacaatt      600

tactggtatt attttgtgaa aggggctatt ttcaatgcgt tcatccatcc ttttcatccc      660

tcaaatctct cattcacgtc cccctcccca tctgcacact ttatctcatt ttccaccctg      720

ctggaatctg agcacttgtg cagttatcag ggctcctgta tttaggaggc tctgggtgtc      780

catgtagggg acgaacagaa acactgcaga cctttataga agaacaattg ataagagtcc      840

tcatacataa agactccatt agtaagccag tgacccagga gcccagacca acagcaaagc      900

agacagtgac catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg      960

tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg     1020

atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc     1080

cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg     1140

accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc     1200

gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg     1260

gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca     1320

tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca     1380

agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg     1440

tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc     1500

ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg     1560

atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc     1620

tgtacaagta gtaaacgttg cagcaagatt aca                                  1653


<210>  408
<211>  100
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Product

<400>  408
cagcaaagca gacagtgacc atggtgagca agggcgagga gctgttcacc ggggtggtgc       60

ccatcctggt cgagctggac ggcgacgtaa acggccacaa                            100


<210>  409
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward Primer

<400>  409
cagcaaagca gacagtgacc                                                   20


<210>  410
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse Primer

<400>  410
ttgtggccgt ttacgtcg                                                     18


<210>  411
<211>  49
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Digested PCR Product(1)

<400>  411
cagcaaagca gacagtgacc atggtgagca agggcgagga gctgttcac                   49


<210>  412
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Digested PCR Product(2)

<400>  412
cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca a                51


<210>  413
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MmeI digested product(1)

<400>  413
gggcgaggag ctgttcac                                                     18


<210>  414
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MmeI digested product(2)

<400>  414
gggtggtgcc catcctgg                                                     18


<210>  415
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7 Promoter


<220>
<221>  misc_feature
<222>  (28)..(29)
<223>  n is a, c, g, or t

<400>  415
cggcgaaatt aatacgactc actatagnn                                         29


<210>  416
<211>  102
<212>  RNA
<213>  Streptococcus pyogenes


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  n is a, c, g, or u

<400>  416
nnnnnnnnnn nnnnnnnnnn guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc       60

cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu uu                         102


<210>  417
<211>  102
<212>  DNA
<213>  Streptococcus pyogenes


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  n is a, c, g, or t

<400>  417
nnnnnnnnnn nnnnnnnnnn gttttagagc tagaaatagc aagttaaaat aaggctagtc       60

cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt tt                         102


<210>  418
<211>  102
<212>  DNA
<213>  Streptococcus pyogenes


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  n is a, c, g, or t

<400>  418
nnnnnnnnnn nnnnnnnnnn caaaatctcg atctttatcg ttcaatttta ttccgatcag       60

gcaatagttg aactttttca ccgtggctca gccacgaaaa aa                         102


<210>  419
<211>  102
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  polynucleotides encoding for a sgRNA and having non-palindromic 
       recognition sites


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  n is a, c, g, or t

<400>  419
nnnnnnnnnn nnnnnnnnnn gttggagagc tagaaatagc aagttccaat aaggctagtc       60

cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt tt                         102


<210>  420
<211>  102
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  polynucleotides encoding for a sgRNA and having non-palindromic 
       recognition sites


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  n is a, c, g, or t

<400>  420
nnnnnnnnnn nnnnnnnnnn caacctctcg atctttatcg ttcaaggtta ttccgatcag       60

gcaatagttg aactttttca ccgtggctca gccacgaaaa aa                         102


<210>  421
<211>  102
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  polynucleotides encoding for a sgRNA and having non-palindromic 
       recognition sites


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  n is a, c, g, or t

<400>  421
nnnnnnnnnn nnnnnnnnnn cggttggagc tagaaatagc aagtcaacct aaggctagtc       60

cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt tt                         102


<210>  422
<211>  102
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  polynucleotides encoding for a sgRNA and having non-palindromic 
       recognition sites


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  n is a, c, g, or t

<400>  422
nnnnnnnnnn nnnnnnnnnn gccaacctcg atctttatcg ttcagttgga ttccgatcag       60

gcaatagttg aactttttca ccgtggctca gccacgaaaa aa                         102


<210>  423
<211>  101
<212>  RNA
<213>  Streptococcus pyogenes


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  n is a, c, g, or u

<400>  423
nnnnnnnnnn nnnnnnnnnn guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc       60

cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu u                          101


<210>  424
<211>  101
<212>  DNA
<213>  Streptococcus pyogenes


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  n is a, c, g, or t

<400>  424
nnnnnnnnnn nnnnnnnnnn gttttagagc tagaaatagc aagttaaaat aaggctagtc       60

cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt t                          101


<210>  425
<211>  101
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  polynucleotides encoding for an sgRNA or crRNA recognized by a 
       CRIPSR Cas9 protein and having non-palindromic recognition site


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  n is a, c, g, or t

<400>  425
nnnnnnnnnn nnnnnnnnnn gttttagagc tagaaatagc aagttaaaat aaggctagtc       60

cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt t                          101


<210>  426
<211>  100
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  polynucleotides encoding for an sgRNA or crRNA recognized by a 
       CRIPSR Cas9 protein and having non-palindromic recognition site


<220>
<221>  misc_feature
<222>  (1)..(19)
<223>  n is a, c, g, or t

<400>  426
nnnnnnnnnn nnnnnnnnng gttggaagct agaaatagca agtccaacta aggctagtcc       60

gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt                            100


<210>  427
<211>  99
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  polynucleotides encoding for an sgRNA or crRNA recognized by a 
       CRIPSR Cas9 protein and having non-palindromic recognition site


<220>
<221>  misc_feature
<222>  (1)..(18)
<223>  n is a, c, g, or t

<400>  427
nnnnnnnnnn nnnnnnnngt gttggagcta gaaatagcaa gtcaacataa ggctagtccg       60

ttatcaactt gaaaaagtgg caccgagtcg gtgcttttt                              99


<210>  428
<211>  99
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  polynucleotides encoding for an sgRNA or crRNA recognized by a 
       CRIPSR Cas9 protein and having non-palindromic recognition site


<220>
<221>  misc_feature
<222>  (1)..(17)
<223>  n is a, c, g, or t

<400>  428
nnnnnnnnnn nnnnnnnccg gttggagcta gaaatagcaa gtcaacctaa ggctagtccg       60

ttatcaactt gaaaaagtgg caccgagtcg gtgcttttt                              99


<210>  429
<211>  99
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  polynucleotides encoding for an sgRNA or crRNA recognized by a 
       CRIPSR Cas9 protein and having non-palindromic recognition site


<220>
<221>  misc_feature
<222>  (1)..(16)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (19)..(19)
<223>  n is a, c, g, or t

<400>  429
nnnnnnnnnn nnnnnnccng gttggagcta gaaatagcaa gtcaacctaa ggctagtccg       60

ttatcaactt gaaaaagtgg caccgagtcg gtgcttttt                              99


<210>  430
<211>  99
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  polynucleotides encoding for an sgRNA or crRNA recognized by a 
       CRIPSR Cas9 protein and having non-palindromic recognition site


<220>
<221>  misc_feature
<222>  (1)..(17)
<223>  n is a, c, g, or t

<400>  430
nnnnnnnnnn nnnnnnncta gttggagcta gaaatagcaa gtcaacctaa ggctagtccg       60

ttatcaactt gaaaaagtgg caccgagtcg gtgcttttt                              99


<210>  431
<211>  101
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  polynucleotides encoding for an sgRNA or crRNA recognized by a 
       CRIPSR Cas9 protein and having non-palindromic recognition site


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  n is a, c, g, or t

<400>  431
nnnnnnnnnn nnnnnnnnnn gtcggagagc tagaaatagc aagttccgat aaggctagtc       60

cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt t                          101


<210>  432
<211>  100
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  polynucleotides encoding for an sgRNA or crRNA recognized by a 
       CRIPSR Cas9 protein and having non-palindromic recognition site


<220>
<221>  misc_feature
<222>  (1)..(19)
<223>  n is a, c, g, or t

<400>  432
nnnnnnnnnn nnnnnnnnng gtcggaagct agaaatagca agtccgacta aggctagtcc       60

gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt                            100


<210>  433
<211>  99
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  polynucleotides encoding for an sgRNA or crRNA recognized by a 
       CRIPSR Cas9 protein and having non-palindromic recognition site


<220>
<221>  misc_feature
<222>  (1)..(18)
<223>  n is a, c, g, or t

<400>  433
nnnnnnnnnn nnnnnnnngt gtcggagcta gaaatagcaa gtcgacataa ggctagtccg       60

ttatcaactt gaaaaagtgg caccgagtcg gtgcttttt                              99


<210>  434
<211>  99
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  polynucleotides encoding for an sgRNA or crRNA recognized by a 
       CRIPSR Cas9 protein and having non-palindromic recognition site


<220>
<221>  misc_feature
<222>  (1)..(17)
<223>  n is a, c, g, or t

<400>  434
nnnnnnnnnn nnnnnnnccg gtcggagcta gaaatagcaa gtcgacctaa ggctagtccg       60

ttatcaactt gaaaaagtgg caccgagtcg gtgcttttt                              99


<210>  435
<211>  99
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  polynucleotides encoding for an sgRNA or crRNA recognized by a 
       CRIPSR Cas9 protein and having non-palindromic recognition site


<220>
<221>  misc_feature
<222>  (1)..(16)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (19)..(19)
<223>  n is a, c, g, or t

<400>  435
nnnnnnnnnn nnnnnnccng gtcggagcta gaaatagcaa gtcgacctaa ggctagtccg       60

ttatcaactt gaaaaagtgg caccgagtcg gtgcttttt                              99


<210>  436
<211>  99
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  polynucleotides encoding for an sgRNA or crRNA recognized by a 
       CRIPSR Cas9 protein and having non-palindromic recognition site


<220>
<221>  misc_feature
<222>  (1)..(17)
<223>  n is a, c, g, or t

<400>  436
nnnnnnnnnn nnnnnnncta gtcggagcta gaaatagcaa gtcgacctaa ggctagtccg       60

ttatcaactt gaaaaagtgg caccgagtcg gtgcttttt                              99


<210>  437
<211>  101
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  polynucleotides encoding for an sgRNA or crRNA recognized by a 
       CRIPSR Cas9 protein and having non-palindromic recognition site


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  n is a, c, g, or t

<400>  437
nnnnnnnnnn nnnnnnnnnn ctcggcgagc tagaaatagc aagtgccgat aaggctagtc       60

cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt t                          101


<210>  438
<211>  100
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  polynucleotides encoding for an sgRNA or crRNA recognized by a 
       CRIPSR Cas9 protein and having non-palindromic recognition site


<220>
<221>  misc_feature
<222>  (1)..(19)
<223>  n is a, c, g, or t

<400>  438
nnnnnnnnnn nnnnnnnnng ctcggcagct agaaatagca agtccgagta aggctagtcc       60

gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt                            100


<210>  439
<211>  99
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  polynucleotides encoding for an sgRNA or crRNA recognized by a 
       CRIPSR Cas9 protein and having non-palindromic recognition site


<220>
<221>  misc_feature
<222>  (1)..(18)
<223>  n is a, c, g, or t

<400>  439
nnnnnnnnnn nnnnnnnngt ctcggcgcta gaaatagcaa gtcgagataa ggctagtccg       60

ttatcaactt gaaaaagtgg caccgagtcg gtgcttttt                              99


<210>  440
<211>  39
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Francisella novicida


<220>
<221>  misc_feature
<222>  (20)..(39)
<223>  n is a, c, g, or u

<400>  440
aauuucuacu guuguagaun nnnnnnnnnn nnnnnnnnn                              39


<210>  441
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Francisella novicida cRNA for CRISPR Cpf1


<220>
<221>  misc_feature
<222>  (20)..(38)
<223>  n is a, c, g, or t

<400>  441
aatttctact gttgtagatn nnnnnnnnnn nnnnnnnn                               38


<210>  442
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  polynucleotides encoding for an sgRNA or crRNA recognized by a 
       CRISPR Cpf1 protein and having non-palindromic recognition site.


<220>
<221>  misc_feature
<222>  (20)..(38)
<223>  n is a, c, g, or t

<400>  442
aattgttggt gttccaactn nnnnnnnnnn nnnnnnnn                               38


<210>  443
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  polynucleotides encoding for an sgRNA or crRNA recognized by a 
       CRISPR Cpf1 protein and having non-palindromic recognition site.


<220>
<221>  misc_feature
<222>  (20)..(37)
<223>  n is a, c, g, or t

<400>  443
aatttgttgt gtccaacatn nnnnnnnnnn nnnnnnn                                37


<210>  444
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  polynucleotides encoding for an sgRNA or crRNA recognized by a 
       CRISPR Cpf1 protein and having non-palindromic recognition site.


<220>
<221>  misc_feature
<222>  (20)..(38)
<223>  n is a, c, g, or t

<400>  444
aattgttggt gttccgactn nnnnnnnnnn nnnnnnnn                               38


<210>  445
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  polynucleotides encoding for an sgRNA or crRNA recognized by a 
       CRISPR Cpf1 protein and having non-palindromic recognition site.


<220>
<221>  misc_feature
<222>  (20)..(37)
<223>  n is a, c, g, or t

<400>  445
aatttgttgt gtccgacatn nnnnnnnnnn nnnnnnn                                37


<210>  446
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  polynucleotides encoding for an sgRNA or crRNA recognized by a 
       CRISPR Cpf1 protein and having non-palindromic recognition site.


<220>
<221>  misc_feature
<222>  (20)..(38)
<223>  n is a, c, g, or t

<400>  446
aattctcggt gtgccgagtn nnnnnnnnnn nnnnnnnn                               38


<210>  447
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  polynucleotides encoding for an sgRNA or crRNA recognized by a 
       CRISPR Cpf1 protein and having non-palindromic recognition site.


<220>
<221>  misc_feature
<222>  (20)..(37)
<223>  n is a, c, g, or t

<400>  447
aatttctcgt ggccgagatn nnnnnnnnnn nnnnnnn                                37


<210>  448
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  polynucleotides encoding for an sgRNA or crRNA recognized by a 
       CRISPR Cpf1 protein and having non-palindromic recognition site.


<220>
<221>  misc_feature
<222>  (22)..(38)
<223>  n is a, c, g, or t

<400>  448
aattgttggt gttccaacta tnnnnnnnnn nnnnnnnn                               38


<210>  449
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  polynucleotides encoding for an sgRNA or crRNA recognized by a 
       CRISPR Cpf1 protein and having non-palindromic recognition site.


<220>
<221>  misc_feature
<222>  (22)..(37)
<223>  n is a, c, g, or t

<400>  449
aatttgttgt gtccaacata tnnnnnnnnn nnnnnnn                                37


<210>  450
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  polynucleotides encoding for an sgRNA or crRNA recognized by a 
       CRISPR Cpf1 protein and having non-palindromic recognition site.


<220>
<221>  misc_feature
<222>  (22)..(38)
<223>  n is a, c, g, or t

<400>  450
aattgttggt gttccgacta tnnnnnnnnn nnnnnnnn                               38


<210>  451
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  polynucleotides encoding for an sgRNA or crRNA recognized by a 
       CRISPR Cpf1 protein and having non-palindromic recognition site.


<220>
<221>  misc_feature
<222>  (22)..(37)
<223>  n is a, c, g, or t

<400>  451
aatttgttgt gtccgacata tnnnnnnnnn nnnnnnn                                37


<210>  452
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  polynucleotides encoding for an sgRNA or crRNA recognized by a 
       CRISPR Cpf1 protein and having non-palindromic recognition site.


<220>
<221>  misc_feature
<222>  (22)..(38)
<223>  n is a, c, g, or t

<400>  452
aattctcggt gtgccgagta tnnnnnnnnn nnnnnnnn                               38


<210>  453
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  polynucleotides encoding for an sgRNA or crRNA recognized by a 
       CRISPR Cpf1 protein and having non-palindromic recognition site.


<220>
<221>  misc_feature
<222>  (22)..(37)
<223>  n is a, c, g, or t

<400>  453
aatttctcgt ggccgagata tnnnnnnnnn nnnnnnn                                37


<210>  454
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward strand of a polynucleotide which encodes an RNA bound by 
       a cas enzyme


<220>
<221>  misc_feature
<222>  (1)..(11)
<223>  Sequence1 (Used in Kit)

<220>
<221>  misc_feature
<222>  (3)..(8)
<223>  Type IIS binding site

<220>
<221>  misc_feature
<222>  (12)..(81)
<223>  Sequence2 (In Plasmid)

<400>  454
ntgtyggagc tagaaatagc aagtcragat aaggctagtc cgttatcaac ttgaaaaagt       60

ggcaccgagt cggtgctttt t                                                 81


<210>  455
<211>  80
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse strand of a polynucleotide which encodes an RNA bound by 
       a cas enzyme


<220>
<221>  misc_feature
<222>  (1)..(74)
<223>  Sequence1 (Used in Kit)

<220>
<221>  misc_feature
<222>  (74)..(79)
<223>  Type IIS binding site

<220>
<221>  misc_feature
<222>  (75)..(80)
<223>  Sequence1 (Used in Kit)

<400>  455
aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct tatctygact       60

tgctatttct agctccraca                                                   80


<210>  456
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward strand of a polynucleotide which encodes an RNA bound by 
       a cas enzyme


<220>
<221>  misc_feature
<222>  (1)..(11)
<223>  Sequence1 (Used in Kit)

<220>
<221>  misc_feature
<222>  (3)..(8)
<223>  Type IIS binding site

<220>
<221>  misc_feature
<222>  (12)..(81)
<223>  Sequence2 (In Plasmid)

<400>  456
ntctyggagc tagaaatagc aagtcragat aaggctagtc cgttatcaac ttgaaaaagt       60

ggcaccgagt cggtgctttt t                                                 81


<210>  457
<211>  80
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse strand of a polynucleotide which encodes an RNA bound by 
       a cas enzyme


<220>
<221>  misc_feature
<222>  (1)..(74)
<223>  Sequence2 (In Plasmid)

<220>
<221>  misc_feature
<222>  (74)..(79)
<223>  Type IIS binding site

<220>
<221>  misc_feature
<222>  (75)..(80)
<223>  Sequence1 (Used in Kit)

<400>  457
aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct tatctygact       60

tgctatttct agctccraga                                                   80


<210>  458
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward strand of a polynucleotide which encodes an RNA bound by 
       a cas enzyme


<220>
<221>  misc_feature
<222>  (3)..(8)
<223>  Type IIS binding site

<400>  458
cgctyggagc tagaaatagc aagtcragct aaggctagtc cgttatcaac ttgaaaaagt       60

ggcaccgagt cggtgctttt t                                                 81


<210>  459
<211>  79
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse strand of a polynucleotide which encodes an RNA bound by 
       a cas enzyme


<220>
<221>  misc_feature
<222>  (74)..(79)
<223>  Type IIS binding site

<400>  459
aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct tagctygact       60

tgctatttct agctccrag                                                    79


<210>  460
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward strand of a polynucleotide which encodes an RNA bound by 
       a cas enzyme


<220>
<221>  misc_feature
<222>  (3)..(8)
<223>  Type IIS binding site

<400>  460
tactyggagc tagaaatagc aagtcragct aaggctagtc cgttatcaac ttgaaaaagt       60

ggcaccgagt cggtgctttt t                                                 81


<210>  461
<211>  79
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse strand of a polynucleotide which encodes an RNA bound by 
       a cas enzyme


<220>
<221>  misc_feature
<222>  (74)..(79)
<223>  Type IIS binding site

<400>  461
aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct tagctygact       60

tgctatttct agctccrag                                                    79


<210>  462
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward strand of a polynucleotide which encodes an RNA bound by 
       a cas enzyme


<220>
<221>  misc_feature
<222>  (3)..(8)
<223>  Type IIS binding site

<400>  462
gtctyggagc tagaaatagc aagtcragct aaggctagtc cgttatcaac ttgaaaaagt       60

ggcaccgagt cggtgctttt t                                                 81


<210>  463
<211>  83
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse strand of a polynucleotide which encodes an RNA bound by 
       a cas enzyme


<220>
<221>  misc_feature
<222>  (74)..(79)
<223>  Type IIS binding site

<400>  463
aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct tagctygact       60

tgctatttct agctccraga cat                                               83


<210>  464
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward strand of a polynucleotide which encodes an RNA bound by 
       a cas enzyme


<220>
<221>  misc_feature
<222>  (3)..(8)
<223>  Type IIS binding site

<400>  464
gtgtyggagc tagaaatagc aagtcragct aaggctagtc cgttatcaac ttgaaaaagt       60

ggcaccgagt cggtgctttt t                                                 81


<210>  465
<211>  83
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse strand of a polynucleotide which encodes an RNA bound by 
       a cas enzyme


<220>
<221>  misc_feature
<222>  (74)..(79)
<223>  Type IIS binding site

<400>  465
aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct tagctygact       60

tgctatttct agctccraca cat                                               83


<210>  466
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward strand of a polynucleotide which encodes an RNA bound by 
       a cas enzyme


<220>
<221>  misc_feature
<222>  (1)..(11)
<223>  Sequence1 (Used in Kit)

<220>
<221>  misc_feature
<222>  (2)..(7)
<223>  Type IIS binding site

<220>
<221>  misc_feature
<222>  (12)..(81)
<223>  Sequence2 (In Plasmid)

<400>  466
cgtyggaagc tagaaatagc aagaccract aaggctagtc cgttatcaac ttgaaaaagt       60

ggcaccgagt cggtgctttt t                                                 81


<210>  467
<211>  79
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse strand of a polynucleotide which encodes an RNA bound by 
       a cas enzyme


<220>
<221>  misc_feature
<222>  (1)..(74)
<223>  Sequence2 (In Plasmid)

<220>
<221>  misc_feature
<222>  (75)..(79)
<223>  Type IIS binding site

<220>
<221>  misc_feature
<222>  (75)..(79)
<223>  Sequence1 (Used in Kit)

<400>  467
aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct tagtyggtct       60

tgctatttct agcttccra                                                    79


<210>  468
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward strand of a polynucleotide which encodes an RNA bound by 
       a cas enzyme


<220>
<221>  misc_feature
<222>  (1)..(11)
<223>  Sequence1 (Used in Kit)

<220>
<221>  misc_feature
<222>  (2)..(7)
<223>  Type IIS binding site

<220>
<221>  misc_feature
<222>  (12)..(81)
<223>  Sequence2 (In Plasmid)

<400>  468
nctyggaagc tagaaatagc aagaccract aaggctagtc cgttatcaac ttgaaaaagt       60

ggcaccgagt cggtgctttt t                                                 81


<210>  469
<211>  80
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse strand of a polynucleotide which encodes an RNA bound by 
       a cas enzyme


<220>
<221>  misc_feature
<222>  (1)..(74)
<223>  Sequence2 (In Plasmid)

<220>
<221>  misc_feature
<222>  (75)..(80)
<223>  Type IIS binding site

<220>
<221>  misc_feature
<222>  (75)..(80)
<223>  Sequence1 (Used in Kit)

<400>  469
aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct tagtyggtct       60

tgctatttct agcttccrag                                                   80


<210>  470
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward strand of a polynucleotide which encodes an RNA bound by 
       a cas enzyme


<220>
<221>  misc_feature
<222>  (1)..(11)
<223>  Sequence1 (Used in Kit)

<220>
<221>  misc_feature
<222>  (2)..(7)
<223>  Type IIS binding site

<220>
<221>  misc_feature
<222>  (12)..(81)
<223>  Sequence2 (In Plasmid)

<400>  470
ngtyggaagc tagaaatagc aagaccract aaggctagtc cgttatcaac ttgaaaaagt       60

ggcaccgagt cggtgctttt t                                                 81


<210>  471
<211>  80
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse strand of a polynucleotide which encodes an RNA bound by 
       a cas enzyme


<220>
<221>  misc_feature
<222>  (1)..(74)
<223>  Sequence2 (In Plasmid)

<220>
<221>  misc_feature
<222>  (75)..(80)
<223>  Type IIS binding site

<220>
<221>  misc_feature
<222>  (75)..(80)
<223>  Sequence1 (Used in Kit)

<400>  471
aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct tagtyggtct       60

tgctatttct agcttccrac                                                   80


<210>  472
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward strand of a polynucleotide which encodes an RNA bound by 
       a cas enzyme


<220>
<221>  misc_feature
<222>  (1)..(11)
<223>  Sequence1 (Used in Kit)

<220>
<221>  misc_feature
<222>  (2)..(7)
<223>  Type IIS binding site

<220>
<221>  misc_feature
<222>  (12)..(81)
<223>  Sequence2 (In Plasmid)

<400>  472
gctyggaagc tagaaatagc aagaccract aaggctagtc cgttatcaac ttgaaaaagt       60

ggcaccgagt cggtgctttt t                                                 81


<210>  473
<211>  83
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse strand of a polynucleotide which encodes an RNA bound by 
       a cas enzyme


<220>
<221>  misc_feature
<222>  (1)..(74)
<223>  Sequence2 (In Plasmid)

<220>
<221>  misc_feature
<222>  (75)..(80)
<223>  Type IIS binding site

<220>
<221>  misc_feature
<222>  (75)..(83)
<223>  Sequence1 (Used in Kit)

<400>  473
aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct tagtyggtct       60

tgctatttct agcttccrag cat                                               83


<210>  474
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward strand of a polynucleotide which encodes an RNA bound by 
       a cas enzyme


<220>
<221>  misc_feature
<222>  (1)..(11)
<223>  Sequence1 (Used in Kit)

<220>
<221>  misc_feature
<222>  (2)..(7)
<223>  Type IIS binding site

<220>
<221>  misc_feature
<222>  (12)..(81)
<223>  Sequence2 (In Plasmid)

<400>  474
ggtyggaagc tagaaatagc aagaccract aaggctagtc cgttatcaac ttgaaaaagt       60

ggcaccgagt cggtgctttt t                                                 81


<210>  475
<211>  83
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse strand of a polynucleotide which encodes an RNA bound by 
       a cas enzyme


<220>
<221>  misc_feature
<222>  (1)..(74)
<223>  Sequence2 (In Plasmid)

<220>
<221>  misc_feature
<222>  (75)..(80)
<223>  Type IIS binding site

<220>
<221>  misc_feature
<222>  (75)..(83)
<223>  Sequence1 (Used in Kit)

<400>  475
aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct tagtyggtct       60

tgctatttct agcttccrac cat                                               83


<210>  476
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward strand of a polynucleotide which encodes an RNA bound by 
       a cas enzyme


<220>
<221>  misc_feature
<222>  (1)..(6)
<223>  Type IIS binding site

<220>
<221>  misc_feature
<222>  (1)..(11)
<223>  Sequence1 (Used in Kit)

<220>
<221>  misc_feature
<222>  (12)..(81)
<223>  Sequence2 (In Plasmid)

<400>  476
ctyggagagc tagaaatagc aagaccract aaggctagtc cgttatcaac ttgaaaaagt       60

ggcaccgagt cggtgctttt t                                                 81


<210>  477
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse strand of a polynucleotide which encodes an RNA bound by 
       a cas enzyme


<220>
<221>  misc_feature
<222>  (1)..(74)
<223>  Sequence2 (In Plasmid)

<220>
<221>  misc_feature
<222>  (75)..(81)
<223>  Sequence1 (Used in Kit)

<220>
<221>  misc_feature
<222>  (76)..(81)
<223>  Type IIS binding site

<400>  477
aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct tagtyggtct       60

tgctatttct agctctccra g                                                 81


<210>  478
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward strand of a polynucleotide which encodes an RNA bound by 
       a cas enzyme


<220>
<221>  misc_feature
<222>  (1)..(6)
<223>  Type IIS binding site

<220>
<221>  misc_feature
<222>  (1)..(11)
<223>  Sequence1 (Used in Kit)

<220>
<221>  misc_feature
<222>  (12)..(81)
<223>  Sequence2 (In Plasmid)

<400>  478
gtyggagagc tagaaatagc aagaccract aaggctagtc cgttatcaac ttgaaaaagt       60

ggcaccgagt cggtgctttt t                                                 81


<210>  479
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse strand of a polynucleotide which encodes an RNA bound by 
       a cas enzyme


<220>
<221>  misc_feature
<222>  (1)..(74)
<223>  Sequence2 (In Plasmid)

<220>
<221>  misc_feature
<222>  (75)..(81)
<223>  Sequence1 (Used in Kit)

<220>
<221>  misc_feature
<222>  (76)..(81)
<223>  Type IIS binding site

<400>  479
aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct tagtyggtct       60

tgctatttct agctctccra c                                                 81


<210>  480
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward strand of a polynucleotide which encodes an RNA bound by 
       a cas enzyme


<220>
<221>  misc_feature
<222>  (4)..(9)
<223>  Type IIS binding site

<400>  480
cgtctcggcc tagaaatagg aagtgagaat aaggctagtc cgttatcaac ttgaaaaagt       60

ggcaccgagt cggtgctttt t                                                 81


<210>  481
<211>  79
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse strand of a polynucleotide which encodes an RNA bound by 
       a cas enzyme


<220>
<221>  misc_feature
<222>  (73)..(78)
<223>  Type IIS binding site

<400>  481
aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct tattctcact       60

tcctatttct aggccgaga                                                    79


<210>  482
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward strand of a polynucleotide which encodes an RNA bound by 
       a cas enzyme


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (4)..(9)
<223>  Type IIS binding site

<400>  482
nttctcggcc tagaaatagg aagtgagaat aaggctagtc cgttatcaac ttgaaaaagt       60

ggcaccgagt cggtgctttt t                                                 81


<210>  483
<211>  80
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse strand of a polynucleotide which encodes an RNA bound by 
       a cas enzyme


<220>
<221>  misc_feature
<222>  (73)..(78)
<223>  Type IIS binding site

<400>  483
aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct tattctcact       60

tcctatttct aggccgagaa                                                   80


<210>  484
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward strand of a polynucleotide which encodes an RNA bound by 
       a cas enzyme


<220>
<221>  misc_feature
<222>  (4)..(9)
<223>  Type IIS binding site

<400>  484
tatctcggcc tagaaatagg aagtgagaat aaggctagtc cgttatcaac ttgaaaaagt       60

ggcaccgagt cggtgctttt t                                                 81


<210>  485
<211>  79
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse strand of a polynucleotide which encodes an RNA bound by 
       a cas enzyme


<220>
<221>  misc_feature
<222>  (73)..(78)
<223>  Type IIS binding site

<400>  485
aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct tattctcact       60

tcctatttct aggccgaga                                                    79


<210>  486
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward strand of a polynucleotide which encodes an RNA bound by 
       a cas enzyme


<220>
<221>  misc_feature
<222>  (4)..(9)
<223>  Type IIS binding site

<400>  486
gttctcggcc tagaaatagg aagtgagaat aaggctagtc cgttatcaac ttgaaaaagt       60

ggcaccgagt cggtgctttt t                                                 81


<210>  487
<211>  83
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse strand of a polynucleotide which encodes an RNA bound by 
       a cas enzyme


<220>
<221>  misc_feature
<222>  (73)..(78)
<223>  Type IIS binding site

<400>  487
aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct tattctcact       60

tcctatttct aggccgagaa cat                                               83


<210>  488
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward strand of a polynucleotide which encodes an RNA bound by 
       a cas enzyme


<220>
<221>  misc_feature
<222>  (1)..(30)
<223>  Sequence1 (Used in Kit)

<220>
<221>  misc_feature
<222>  (3)..(8)
<223>  Type IIS binding site

<220>
<221>  misc_feature
<222>  (31)..(81)
<223>  Sequence2 (In Plasmid)

<400>  488
cgctcggcgc tagaaatagc aaatcgagct aaggctagtc cgttatcaac ttgaaaaagt       60

ggcaccgagt cggtgctttt t                                                 81


<210>  489
<211>  79
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse strand of a polynucleotide which encodes an RNA bound by 
       a cas enzyme


<220>
<221>  misc_feature
<222>  (1)..(55)
<223>  Sequence2 (In Plasmid)

<220>
<221>  misc_feature
<222>  (56)..(79)
<223>  Sequence1 (Used in Kit)

<220>
<221>  misc_feature
<222>  (74)..(79)
<223>  Type IIS binding site

<400>  489
aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct tagctcgatt       60

tgctatttct agcgccgag                                                    79


<210>  490
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward strand of a polynucleotide which encodes an RNA bound by 
       a cas enzyme


<220>
<221>  misc_feature
<222>  (1)..(30)
<223>  Sequence1 (Used in Kit)

<220>
<221>  misc_feature
<222>  (3)..(8)
<223>  Type IIS binding site

<220>
<221>  misc_feature
<222>  (31)..(81)
<223>  Sequence2 (In Plasmid)

<400>  490
ntctcggcgc tagaaatagc aaatcgagct aaggctagtc cgttatcaac ttgaaaaagt       60

ggcaccgagt cggtgctttt t                                                 81


<210>  491
<211>  80
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse strand of a polynucleotide which encodes an RNA bound by 
       a cas enzyme


<220>
<221>  misc_feature
<222>  (1)..(55)
<223>  Sequence2 (In Plasmid)

<220>
<221>  misc_feature
<222>  (56)..(80)
<223>  Sequence1 (Used in Kit)

<220>
<221>  misc_feature
<222>  (74)..(79)
<223>  Type IIS binding site

<400>  491
aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct tagctcgatt       60

tgctatttct agcgccgaga                                                   80


<210>  492
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward strand of a polynucleotide which encodes an RNA bound by 
       a cas enzyme


<220>
<221>  misc_feature
<222>  (1)..(30)
<223>  Sequence1 (Used in Kit)

<220>
<221>  misc_feature
<222>  (3)..(8)
<223>  Type IIS binding site

<220>
<221>  misc_feature
<222>  (31)..(81)
<223>  Sequence2 (In Plasmid)

<400>  492
tactcggcgc tagaaatagc aaatcgagct aaggctagtc cgttatcaac ttgaaaaagt       60

ggcaccgagt cggtgctttt t                                                 81


<210>  493
<211>  79
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse strand of a polynucleotide which encodes an RNA bound by 
       a cas enzyme


<220>
<221>  misc_feature
<222>  (1)..(55)
<223>  Sequence2 (In Plasmid)

<220>
<221>  misc_feature
<222>  (56)..(79)
<223>  Sequence1 (Used in Kit)

<220>
<221>  misc_feature
<222>  (74)..(79)
<223>  Type IIS binding site

<400>  493
aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct tagctcgatt       60

tgctatttct agcgccgag                                                    79


<210>  494
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward strand of a polynucleotide which encodes an RNA bound by 
       a cas enzyme


<220>
<221>  misc_feature
<222>  (1)..(30)
<223>  Sequence1 (Used in Kit)

<220>
<221>  misc_feature
<222>  (3)..(8)
<223>  Type IIS binding site

<220>
<221>  misc_feature
<222>  (31)..(81)
<223>  Sequence2 (In Plasmid)

<400>  494
gtctcggcgc tagaaatagc aaatcgagct aaggctagtc cgttatcaac ttgaaaaagt       60

ggcaccgagt cggtgctttt t                                                 81


<210>  495
<211>  83
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse strand of a polynucleotide which encodes an RNA bound by 
       a cas enzyme


<220>
<221>  misc_feature
<222>  (1)..(55)
<223>  Sequence2 (In Plasmid)

<220>
<221>  misc_feature
<222>  (56)..(83)
<223>  Sequence1 (Used in Kit)

<220>
<221>  misc_feature
<222>  (74)..(79)
<223>  Type IIS binding site

<400>  495
aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct tagctcgatt       60

tgctatttct agcgccgaga cat                                               83


<210>  496
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward strand of a polynucleotide which encodes an RNA bound by 
       a cas enzyme


<220>
<221>  misc_feature
<222>  (1)..(11)
<223>  Sequence1 (Used in Kit)

<220>
<221>  misc_feature
<222>  (2)..(7)
<223>  Type IIS binding site

<220>
<221>  misc_feature
<222>  (12)..(81)
<223>  Sequence2 (In Plasmid)

<400>  496
nctcggcagc tagaaatagc aagcccgagt aaggctagtc cgttatcaac ttgaaaaagt       60

ggcaccgagt cggtgctttt t                                                 81


<210>  497
<211>  80
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse strand of a polynucleotide which encodes an RNA bound by 
       a cas enzyme


<220>
<221>  misc_feature
<222>  (1)..(74)
<223>  Sequence2 (In Plasmid)

<220>
<221>  misc_feature
<222>  (75)..(80)
<223>  Type IIS binding site

<220>
<221>  misc_feature
<222>  (75)..(80)
<223>  Sequence1 (Used in Kit)

<400>  497
aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct tactcgggct       60

tgctatttct agctgccgag                                                   80


<210>  498
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward strand of a polynucleotide which encodes an RNA bound by 
       a cas enzyme


<220>
<221>  misc_feature
<222>  (1)..(11)
<223>  Sequence1 (Used in Kit)

<220>
<221>  misc_feature
<222>  (2)..(7)
<223>  Type IIS binding site

<220>
<221>  misc_feature
<222>  (12)..(81)
<223>  Sequence2 (In Plasmid)

<400>  498
gctcggcagc tagaaatagc aagcccgagt aaggctagtc cgttatcaac ttgaaaaagt       60

ggcaccgagt cggtgctttt t                                                 81


<210>  499
<211>  83
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse strand of a polynucleotide which encodes an RNA bound by 
       a cas enzyme


<220>
<221>  misc_feature
<222>  (1)..(74)
<223>  Sequence2 (In Plasmid)

<220>
<221>  misc_feature
<222>  (75)..(80)
<223>  Type IIS binding site

<220>
<221>  misc_feature
<222>  (75)..(83)
<223>  Sequence1 (Used in Kit)

<400>  499
aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct tactcgggct       60

tgctatttct agctgccgag cat                                               83


<210>  500
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward strand of a polynucleotide which encodes an RNA bound by 
       a cas enzyme


<220>
<221>  misc_feature
<222>  (1)..(6)
<223>  Type IIS binding site

<220>
<221>  misc_feature
<222>  (1)..(11)
<223>  Sequence1 (Used in Kit)

<220>
<221>  misc_feature
<222>  (12)..(81)
<223>  Sequence2 (In Plasmid)

<400>  500
ctcggcgagc tagaaatagc aagtgccgat aaggctagtc cgttatcaac ttgaaaaagt       60

ggcaccgagt cggtgctttt t                                                 81


<210>  501
<211>  83
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse strand of a polynucleotide which encodes an RNA bound by 
       a cas enzyme


<220>
<221>  misc_feature
<222>  (1)..(74)
<223>  Sequence2 (In Plasmid)

<220>
<221>  misc_feature
<222>  (75)..(83)
<223>  Sequence1 (Used in Kit)

<220>
<221>  misc_feature
<222>  (76)..(81)
<223>  Type IIS binding site

<400>  501
aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct tatcggcact       60

tgctatttct agctcgccga gat                                               83


<210>  502
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  crRNA


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  n is a, c, g, or t

<400>  502
ntgtyggagc tatgctgttt tg                                                22


<210>  503
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  crRNA

<400>  503
caaaacagca tagctccrac a                                                 21


<210>  504
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  crRNA


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  n is a, c, g, or t

<400>  504
ntctyggagc tatgctgttt tg                                                22


<210>  505
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  crRNA

<400>  505
caaaacagca tagctccrag a                                                 21


<210>  506
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  crRNA

<400>  506
cgctyggagc tatgctgttt tg                                                22


<210>  507
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  crRNA

<400>  507
caaaacagca tagctccrag                                                   20


<210>  508
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  crRNA

<400>  508
tactyggagc tatgctgttt tg                                                22


<210>  509
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  crRNA

<400>  509
caaaacagca tagctccrag                                                   20


<210>  510
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  crRNA

<400>  510
gtctyggagc tatgctgttt tg                                                22


<210>  511
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  crRNA

<400>  511
caaaacagca tagctccrag acat                                              24


<210>  512
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  crRNA

<400>  512
gtgtyggagc tatgctgttt tg                                                22


<210>  513
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  crRNA

<400>  513
caaaacagca tagctccrac acat                                              24


<210>  514
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  crRNA

<400>  514
cgtyggaagc tatgctgttt tg                                                22


<210>  515
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  crRNA

<400>  515
caaaacagca tagcttccra                                                   20


<210>  516
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  crRNA


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  n is a, c, g, or t

<400>  516
nctyggaagc tatgctgttt tg                                                22


<210>  517
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  crRNA

<400>  517
caaaacagca tagcttccra g                                                 21


<210>  518
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  crRNA


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  n is a, c, g, or t

<400>  518
ngtyggaagc tatgctgttt tg                                                22


<210>  519
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  crRNA

<400>  519
caaaacagca tagcttccra c                                                 21


<210>  520
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  crRNA

<400>  520
gctyggaagc tatgctgttt tg                                                22


<210>  521
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  crRNA

<400>  521
caaaacagca tagcttccra gcat                                              24


<210>  522
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  crRNA

<400>  522
ggtyggaagc tatgctgttt tg                                                22


<210>  523
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  crRNA

<400>  523
caaaacagca tagcttccra ccat                                              24


<210>  524
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  crRNA

<400>  524
ctyggagagc tatgctgttt tg                                                22


<210>  525
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  crRNA

<400>  525
caaaacagca tagctctccr agat                                              24


<210>  526
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  crRNA

<400>  526
gtyggagagc tatgctgttt tg                                                22


<210>  527
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  crRNA

<400>  527
caaaacagca tagctctccr acat                                              24


<210>  528
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  crRNA

<400>  528
cgtctcggcc tatgctgttt tg                                                22


<210>  529
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  crRNA

<400>  529
caaaacagca taggccgaga                                                   20


<210>  530
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  crRNA


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  n is a, c, g, or t

<400>  530
nttctcggcc tatgctgttt tg                                                22


<210>  531
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  crRNA

<400>  531
caaaacagca taggccgaga a                                                 21


<210>  532
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  crRNA

<400>  532
tatctcggcc tatgctgttt tg                                                22


<210>  533
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  crRNA

<400>  533
caaaacagca taggccgaga                                                   20


<210>  534
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  crRNA

<400>  534
gttctcggcc tatgctgttt tg                                                22


<210>  535
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  crRNA

<400>  535
caaaacagca taggccgaga acat                                              24


<210>  536
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  crRNA

<400>  536
cgctcggcgc tatgctgttt tg                                                22


<210>  537
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  crRNA

<400>  537
caaaacagca tagcgccgag                                                   20


<210>  538
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  crRNA


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  n is a, c, g, or t

<400>  538
ntctcggcgc tatgctgttt tg                                                22


<210>  539
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  crRNA

<400>  539
caaaacagca tagcgccgag a                                                 21


<210>  540
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  crRNA

<400>  540
tactcggcgc tatgctgttt tg                                                22


<210>  541
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  crRNA

<400>  541
caaaacagca tagcgccgag                                                   20


<210>  542
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  crRNA

<400>  542
gtctcggcgc tatgctgttt tg                                                22


<210>  543
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  crRNA

<400>  543
caaaacagca tagcgccgag acat                                              24


<210>  544
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  crRNA


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  n is a, c, g, or t

<400>  544
nctcggcagc tatgctgttt tg                                                22


<210>  545
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  crRNA

<400>  545
caaaacagca tagctgccga g                                                 21


<210>  546
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  crRNA

<400>  546
gctcggcagc tatgctgttt tg                                                22


<210>  547
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  crRNA

<400>  547
caaaacagca tagctgccga gcat                                              24


<210>  548
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  crRNA

<400>  548
ctcggcgagc tatgctgttt tg                                                22


<210>  549
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  crRNA

<400>  549
caaaacagca tagctcgccg agat                                              24


<210>  550
<211>  84
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  trascrRNA

<400>  550
ggaaccattc aaaacagcat agcaagtcra gataaggcta gtccgttatc aacttgaaaa       60

agtggcaccg agtcggtgct tttt                                              84


<210>  551
<211>  84
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  trascrRNA

<400>  551
aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct tatctygact       60

tgctatgctg ttttgaatgg ttcc                                              84


<210>  552
<211>  84
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  trascrRNA

<400>  552
ggaaccattc aaaacagcat agcaagtcra gataaggcta gtccgttatc aacttgaaaa       60

agtggcaccg agtcggtgct tttt                                              84


<210>  553
<211>  84
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  trascrRNA

<400>  553
aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct tatctygact       60

tgctatgctg ttttgaatgg ttcc                                              84


<210>  554
<211>  84
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  trascrRNA

<400>  554
ggaaccattc aaaacagcat agcaagtcra gctaaggcta gtccgttatc aacttgaaaa       60

agtggcaccg agtcggtgct tttt                                              84


<210>  555
<211>  84
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  trascrRNA

<400>  555
aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct tagctygact       60

tgctatgctg ttttgaatgg ttcc                                              84


<210>  556
<211>  84
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  trascrRNA

<400>  556
ggaaccattc aaaacagcat agcaagtcra gctaaggcta gtccgttatc aacttgaaaa       60

agtggcaccg agtcggtgct tttt                                              84


<210>  557
<211>  84
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  trascrRNA

<400>  557
aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct tagctygact       60

tgctatgctg ttttgaatgg ttcc                                              84


<210>  558
<211>  84
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  trascrRNA

<400>  558
ggaaccattc aaaacagcat agcaagtcra gctaaggcta gtccgttatc aacttgaaaa       60

agtggcaccg agtcggtgct tttt                                              84


<210>  559
<211>  84
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  trascrRNA

<400>  559
aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct tagctygact       60

tgctatgctg ttttgaatgg ttcc                                              84


<210>  560
<211>  84
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  trascrRNA

<400>  560
ggaaccattc aaaacagcat agcaagtcra gctaaggcta gtccgttatc aacttgaaaa       60

agtggcaccg agtcggtgct tttt                                              84


<210>  561
<211>  84
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  trascrRNA

<400>  561
aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct tagctygact       60

tgctatgctg ttttgaatgg ttcc                                              84


<210>  562
<211>  84
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  trascrRNA

<400>  562
ggaaccattc aaaacagcat agcaagaccr actaaggcta gtccgttatc aacttgaaaa       60

agtggcaccg agtcggtgct tttt                                              84


<210>  563
<211>  84
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  trascrRNA

<400>  563
aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct tagtyggtct       60

tgctatgctg ttttgaatgg ttcc                                              84


<210>  564
<211>  84
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  trascrRNA

<400>  564
ggaaccattc aaaacagcat agcaagaccr actaaggcta gtccgttatc aacttgaaaa       60

agtggcaccg agtcggtgct tttt                                              84


<210>  565
<211>  84
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  trascrRNA

<400>  565
aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct tagtyggtct       60

tgctatgctg ttttgaatgg ttcc                                              84


<210>  566
<211>  84
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  trascrRNA

<400>  566
ggaaccattc aaaacagcat agcaagaccr actaaggcta gtccgttatc aacttgaaaa       60

agtggcaccg agtcggtgct tttt                                              84


<210>  567
<211>  84
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  trascrRNA

<400>  567
aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct tagtyggtct       60

tgctatgctg ttttgaatgg ttcc                                              84


<210>  568
<211>  84
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  trascrRNA

<400>  568
ggaaccattc aaaacagcat agcaagaccr actaaggcta gtccgttatc aacttgaaaa       60

agtggcaccg agtcggtgct tttt                                              84


<210>  569
<211>  84
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  trascrRNA

<400>  569
aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct tagtyggtct       60

tgctatgctg ttttgaatgg ttcc                                              84


<210>  570
<211>  84
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  trascrRNA

<400>  570
ggaaccattc aaaacagcat agcaagaccr actaaggcta gtccgttatc aacttgaaaa       60

agtggcaccg agtcggtgct tttt                                              84


<210>  571
<211>  84
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  trascrRNA

<400>  571
aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct tagtyggtct       60

tgctatgctg ttttgaatgg ttcc                                              84


<210>  572
<211>  84
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  trascrRNA

<400>  572
ggaaccattc aaaacagcat agcaagaccr actaaggcta gtccgttatc aacttgaaaa       60

agtggcaccg agtcggtgct tttt                                              84


<210>  573
<211>  84
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  trascrRNA

<400>  573
aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct tagtyggtct       60

tgctatgctg ttttgaatgg ttcc                                              84


<210>  574
<211>  84
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  trascrRNA

<400>  574
ggaaccattc aaaacagcat agcaagaccr actaaggcta gtccgttatc aacttgaaaa       60

agtggcaccg agtcggtgct tttt                                              84


<210>  575
<211>  84
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  trascrRNA

<400>  575
aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct tagtyggtct       60

tgctatgctg ttttgaatgg ttcc                                              84


<210>  576
<211>  84
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  trascrRNA

<400>  576
ggaaccattc aaaacagcat aggaagtgag aataaggcta gtccgttatc aacttgaaaa       60

agtggcaccg agtcggtgct tttt                                              84


<210>  577
<211>  84
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  trascrRNA

<400>  577
aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct tattctcact       60

tcctatgctg ttttgaatgg ttcc                                              84


<210>  578
<211>  84
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  trascrRNA

<400>  578
ggaaccattc aaaacagcat aggaagtgag aataaggcta gtccgttatc aacttgaaaa       60

agtggcaccg agtcggtgct tttt                                              84


<210>  579
<211>  84
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  trascrRNA

<400>  579
aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct tattctcact       60

tcctatgctg ttttgaatgg ttcc                                              84


<210>  580
<211>  84
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  trascrRNA

<400>  580
ggaaccattc aaaacagcat aggaagtgag aataaggcta gtccgttatc aacttgaaaa       60

agtggcaccg agtcggtgct tttt                                              84


<210>  581
<211>  84
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  trascrRNA

<400>  581
aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct tattctcact       60

tcctatgctg ttttgaatgg ttcc                                              84


<210>  582
<211>  84
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  trascrRNA

<400>  582
ggaaccattc aaaacagcat aggaagtgag aataaggcta gtccgttatc aacttgaaaa       60

agtggcaccg agtcggtgct tttt                                              84


<210>  583
<211>  84
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  trascrRNA

<400>  583
aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct tattctcact       60

tcctatgctg ttttgaatgg ttcc                                              84


<210>  584
<211>  84
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  trascrRNA

<400>  584
ggaaccattc aaaacagcat agcaaatcga gctaaggcta gtccgttatc aacttgaaaa       60

agtggcaccg agtcggtgct tttt                                              84


<210>  585
<211>  84
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  trascrRNA

<400>  585
aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct tagctcgatt       60

tgctatgctg ttttgaatgg ttcc                                              84


<210>  586
<211>  84
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  trascrRNA

<400>  586
ggaaccattc aaaacagcat agcaaatcga gctaaggcta gtccgttatc aacttgaaaa       60

agtggcaccg agtcggtgct tttt                                              84


<210>  587
<211>  84
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  trascrRNA

<400>  587
aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct tagctcgatt       60

tgctatgctg ttttgaatgg ttcc                                              84


<210>  588
<211>  84
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  trascrRNA

<400>  588
ggaaccattc aaaacagcat agcaaatcga gctaaggcta gtccgttatc aacttgaaaa       60

agtggcaccg agtcggtgct tttt                                              84


<210>  589
<211>  84
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  trascrRNA

<400>  589
aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct tagctcgatt       60

tgctatgctg ttttgaatgg ttcc                                              84


<210>  590
<211>  84
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  trascrRNA

<400>  590
ggaaccattc aaaacagcat agcaaatcga gctaaggcta gtccgttatc aacttgaaaa       60

agtggcaccg agtcggtgct tttt                                              84


<210>  591
<211>  84
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  trascrRNA

<400>  591
aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct tagctcgatt       60

tgctatgctg ttttgaatgg ttcc                                              84


<210>  592
<211>  84
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  trascrRNA

<400>  592
ggaaccattc aaaacagcat agcaagcccg agtaaggcta gtccgttatc aacttgaaaa       60

agtggcaccg agtcggtgct tttt                                              84


<210>  593
<211>  84
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  trascrRNA

<400>  593
aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct tactcgggct       60

tgctatgctg ttttgaatgg ttcc                                              84


<210>  594
<211>  84
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  trascrRNA

<400>  594
ggaaccattc aaaacagcat agcaagcccg agtaaggcta gtccgttatc aacttgaaaa       60

agtggcaccg agtcggtgct tttt                                              84


<210>  595
<211>  84
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  trascrRNA

<400>  595
aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct tactcgggct       60

tgctatgctg ttttgaatgg ttcc                                              84


<210>  596
<211>  84
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  trascrRNA

<400>  596
ggaaccattc aaaacagcat agcaagtgcc gataaggcta gtccgttatc aacttgaaaa       60

agtggcaccg agtcggtgct tttt                                              84


<210>  597
<211>  84
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  trascrRNA

<400>  597
aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct tatcggcact       60

tgctatgctg ttttgaatgg ttcc                                              84


