                         SEQUENCE LISTING

<110>  ONCOSIMIS BIOTECH PRIVATE LIMITED
 
<120>  METHOD FOR CONTINOUS PRODUCTION OF RECOMBINANT GLP-1 PEPTIDE BY 
       BACTERIA

<130>  Sudarshan -GLP1

<160>  6     

<170>  PatentIn version 3.5

<210>  1
<211>  93
<212>  DNA
<213>  Homo sapiens

<400>  1
cacgcggaag gcaccttcac cagcgatgtg agcagctacc tggagggtca ggcggcgaaa       60

gaatttatcg cgtggctggt tcgtggtcgt ggc                                    93


<210>  2
<211>  99
<212>  DNA
<213>  Escherichia coli

<400>  2
gcgaacaacg aaaccagcaa gagcgtgacc tttccgaaat gcgaagatct ggatgcggcg       60

ggtattgcgg cgagcgttaa gcgtgactac cagcaaaac                              99


<210>  3
<211>  99
<212>  DNA
<213>  Escherichia coli

<400>  3
gcgaataatg agaccagcaa aagcgtgacc tttccgaagg cggaggacct ggatgcggcg       60

ggtattgcgg cgagcgttaa acgtgactac cagcaaaac                              99


<210>  4
<211>  33
<212>  PRT
<213>  Escherichia coli

<400>  4

Ala Asn Asn Glu Thr Ser Lys Ser Val Thr Phe Pro Lys Cys Glu Asp 
1               5                   10                  15      


Leu Asp Ala Ala Gly Ile Ala Ala Ser Val Lys Arg Asp Tyr Gln Gln 
            20                  25                  30          


Asn 
    


<210>  5
<211>  33
<212>  PRT
<213>  Escherichia coli

<400>  5

Ala Asn Asn Glu Thr Ser Lys Ser Val Thr Phe Pro Lys Ala Glu Asp 
1               5                   10                  15      


Leu Asp Ala Ala Gly Ile Ala Ala Ser Val Lys Arg Asp Tyr Gln Gln 
            20                  25                  30          


Asn 
    


<210>  6
<211>  6793
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Bacterial Expression vector encoding recombinant GLP-1 peptide in
       conjugation with secretory sequence

<400>  6
tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg       60

cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc      120

ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg      180

gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc      240

acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt      300

ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc      360

ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta      420

acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt      480

tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta      540

tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat      600

tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa      660

actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc      720

gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga      780

aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc      840

agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac      900

cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac      960

aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat     1020

tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag     1080

tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca     1140

taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac     1200

ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg     1260

tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca     1320

tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcataacac     1380

cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa aatcccttaa     1440

cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga     1500

gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg     1560

gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc     1620

agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag     1680

aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc     1740

agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg     1800

cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac     1860

accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga     1920

aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt     1980

ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag     2040

cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg     2100

gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta     2160

tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc     2220

agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg     2280

tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta     2340

caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg     2400

ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct     2460

gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag     2520

gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc     2580

gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag     2640

aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt     2700

ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa     2760

acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg     2820

ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg     2880

tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc     2940

tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta     3000

cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca     3060

gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc     3120

ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc     3180

catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa     3240

ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc     3300

gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac     3360

gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca     3420

ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta     3480

atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa     3540

cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat     3600

tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca     3660

ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa     3720

aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt     3780

atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg     3840

cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca     3900

gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta     3960

tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg     4020

agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat     4080

gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct     4140

ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg     4200

catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat     4260

tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc     4320

tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca     4380

gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg     4440

ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt     4500

tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg     4560

catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct     4620

cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga     4680

tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg     4740

ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc     4800

ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg     4860

cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg     4920

gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatcta tacgaaacgg     4980

gaatgcggta attacgcttt gtttttataa gtcagatttt aatttttatt ggttaacata     5040

acgaaaggta aaatacataa ggcttactaa aagccagata acagtatgcg tatttgcgcg     5100

ctgatttttg cggtataaga atatatactg atatgtatac ccgaagtatg tcaaaaagag     5160

gtgtgctatg aagcagcgta ttacagtgac agttgacagc gacagctatc agttgctcaa     5220

ggcatatgat gtcaatatct ccggtctggt aagcacaacc atgcagaatg aagcccgtcg     5280

tctgcgtgcc gaacgctgga aagcggaaaa tcaggaaggg atggctgagg tcgcccggtt     5340

tattgaaatg aacggctctt ttgctgacga gaacagggac tggtgaaatg cagtttaagg     5400

tttacaccta taaaagagag agccgttatc gtctgtttgt ggatgtacag agtgatatta     5460

ttgacacgcc cgggcgacgg atggtgatcc ccctggccag tgcacgtctg ctgtcagata     5520

aagtctcccg tgaactttac ccggtggtgc atatcgggga tgaaagctgg cgcatgatga     5580

ccaccgatat ggccagtgtg ccggtctccg ttatcgggga agaagtggct gatctcagcc     5640

accgcgaaaa tgacatcaaa aacgccatta acctgatgtt ctggggaata taaatgtcag     5700

gctccgttat acacagccag tctgcagcga tcccgcgaaa tttgacaatt aatcatcggc     5760

tcgtataatg tgtggaattg tgagcggata acaattcccc tctagaaata attttgttta     5820

actttaagaa ggagatatac atatgatgaa acgtaatatc ctggcggtga ttgttccggc     5880

gctgctggtt gcgggcaccg cgaatgcggc gaataatgag accagcaaaa gcgtgacctt     5940

tccgaaggcg gaggacctgg atgcggcggg tattgcggcg agcgttaaac gtgactacca     6000

gcaaaacggt ggcagcggtg gcagcggtag ccaccatcat catcaccaca gcagcggtgg     6060

cagcggtacc gactataagg acgatgacga taaacacgcg gaaggcacct ttaccagcga     6120

tgtgagcagc tacctggagg gtcaagcggc gaaggagttc attgcgtggc tggtgcgtgg     6180

tcgtggctaa tagtgagcgg ccgcggctgt tttggcggat gagagaagat tttcagcctg     6240

atacagatta aatcagaacg cagaagcggt ctgataaaac agaatttgcc tggcggcagt     6300

agcgcggtgg tcccacctga ccccatgccg aactcagaag tgaaacgccg tagcgccgat     6360

ggtagtgtgg ggtctcccca tgcgagagta gggaactgcc aggcatcaaa taaaacgaaa     6420

ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcct     6480

gagtaggaca aatccgccgg gagcggattt gaacgttgcg aagcaacggc ccggagggtg     6540

gcgggcagga cgcccgccat aaactgccag gcatcaaatt aagcagaagg ccatcctgac     6600

ggatggcctt tttgcgtttc tacaaactct ctcgagcacc accaccacca ccactgagat     6660

ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa     6720

ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga     6780

actatatccg gat                                                        6793


