                         SEQUENCE LISTING

<110>  Deutsches Krebsforschungszentrum
 
<120>  Use of BMMF Rep Protein as Biomarker for Breast Cancer

<130>  K 3654EP

<150> 
<151>  

<160>  15    

<170>  PatentIn version 3.5

<210>  1
<211>  324
<212>  PRT
<213>  artificial sequence

<220>
<223>  MSBI1 Rep protein

<400>  1

Met Ser Asp Leu Ile Val Lys Asp Asn Ala Leu Met Asn Ala Ser Tyr 
1               5                   10                  15      


Asn Leu Ala Leu Val Glu Gln Arg Leu Ile Leu Leu Ala Ile Ile Glu 
            20                  25                  30          


Ala Arg Glu Thr Gly Lys Gly Ile Asn Ala Asn Asp Pro Leu Thr Val 
        35                  40                  45              


His Ala Ser Ser Tyr Ile Asn Gln Phe Asn Val Glu Arg His Thr Ala 
    50                  55                  60                  


Tyr Gln Ala Leu Lys Asp Ala Cys Lys Asp Leu Phe Ala Arg Gln Phe 
65                  70                  75                  80  


Ser Tyr Gln Glu Lys Arg Glu Arg Gly Arg Ile Asn Ile Thr Ser Arg 
                85                  90                  95      


Trp Val Ser Gln Ile Gly Tyr Met Asp Asp Thr Ala Thr Val Glu Ile 
            100                 105                 110         


Ile Phe Ala Pro Ala Val Val Pro Leu Ile Thr Arg Leu Glu Glu Gln 
        115                 120                 125             


Phe Thr Gln Tyr Asp Ile Glu Gln Ile Ser Gly Leu Ser Ser Ala Tyr 
    130                 135                 140                 


Ala Val Arg Met Tyr Glu Leu Leu Ile Cys Trp Arg Ser Thr Gly Lys 
145                 150                 155                 160 


Thr Pro Ile Ile Glu Leu Asp Glu Phe Arg Lys Arg Ile Gly Val Leu 
                165                 170                 175     


Asp Thr Glu Tyr Thr Arg Thr Asp Asn Leu Lys Met Arg Val Ile Glu 
            180                 185                 190         


Leu Ala Leu Lys Gln Ile Asn Glu His Thr Asp Ile Thr Ala Ser Tyr 
        195                 200                 205             


Glu Gln His Lys Lys Gly Arg Val Ile Thr Gly Phe Ser Phe Lys Phe 
    210                 215                 220                 


Lys His Lys Lys Gln Asn Ser Asp Lys Thr Pro Lys Asn Ser Asp Ser 
225                 230                 235                 240 


Ser Pro Arg Ile Val Lys His Ser Gln Ile Pro Thr Asn Ile Val Lys 
                245                 250                 255     


Gln Pro Glu Asn Ala Lys Met Ser Asp Leu Glu His Arg Ala Ser Arg 
            260                 265                 270         


Val Thr Gly Glu Ile Met Arg Asn Arg Leu Ser Asp Arg Phe Lys Gln 
        275                 280                 285             


Gly Asp Glu Ser Ala Ile Asp Met Met Lys Arg Ile Gln Ser Glu Ile 
    290                 295                 300                 


Ile Thr Asp Ala Ile Ala Asp Gln Trp Glu Ser Lys Leu Glu Glu Phe 
305                 310                 315                 320 


Gly Val Val Phe 
                


<210>  2
<211>  18
<212>  PRT
<213>  artificial sequence

<220>
<223>  Rep peptide

<400>  2

Glu Ala Arg Glu Thr Gly Lys Gly Ile Asn Ala Asn Asp Pro Leu Thr 
1               5                   10                  15      


Val His 
        


<210>  3
<211>  20
<212>  PRT
<213>  artificial sequence

<220>
<223>  Rep peptide

<400>  3

Lys Gln Ile Asn Glu His Thr Asp Ile Thr Ala Ser Tyr Glu His Lys 
1               5                   10                  15      


Lys Gly Arg Thr 
            20  


<210>  4
<211>  8
<212>  PRT
<213>  artificial sequence

<220>
<223>  His tag

<400>  4

Gly Ala His His His His His His 
1               5               


<210>  5
<211>  11
<212>  PRT
<213>  artificial sequence

<220>
<223>  T7 tag

<400>  5

Met Ala Ser Met Thr Gly Gly Gln Gln Met Gly 
1               5                   10      


<210>  6
<211>  8
<212>  PRT
<213>  artificial sequence

<220>
<223>  FLAG tag

<400>  6

Asp Tyr Lys Asp Asp Asp Asp Lys 
1               5               


<210>  7
<211>  8
<212>  PRT
<213>  artificial sequence

<220>
<223>  Trep II tag

<400>  7

Trp Ser His Pro Gln Phe Glu Lys 
1               5               


<210>  8
<211>  319
<212>  PRT
<213>  artificial sequence

<220>
<223>  MSBI2.176

<400>  8

Met Ser Lys Leu Val Val Lys Asp Asn Ala Leu Met Asn Ala Ser Tyr 
1               5                   10                  15      


Asn Leu Asp Leu Val Glu Gln Arg Leu Ile Leu Leu Ala Ile Ile Glu 
            20                  25                  30          


Ala Arg Glu Ser Gly Lys Gly Ile Asn Ala Asn Asp Pro Leu Thr Val 
        35                  40                  45              


His Ala Glu Ser Tyr Ile Asn Gln Phe Gly Val His Arg Val Thr Ala 
    50                  55                  60                  


Tyr Gln Ala Leu Lys Asp Ala Cys Asp Asn Leu Phe Ala Arg Gln Phe 
65                  70                  75                  80  


Ser Tyr Gln Ser Lys Ser Glu Lys Gly Asn Ile Gln Asn His Arg Ser 
                85                  90                  95      


Arg Trp Val Ser Glu Ile Ile Tyr Ile Asp Thr Glu Ala Thr Val Lys 
            100                 105                 110         


Ile Ile Phe Ala Pro Ala Ile Val Pro Leu Ile Thr Arg Leu Glu Glu 
        115                 120                 125             


Gln Phe Thr Lys Tyr Asp Ile Glu Gln Ile Ser Asp Leu Ser Ser Ala 
    130                 135                 140                 


Tyr Ala Ile Arg Leu Tyr Glu Leu Leu Ile Cys Trp Arg Ser Thr Gly 
145                 150                 155                 160 


Lys Thr Pro Ile Ile Gly Leu Gly Glu Phe Arg Asn Arg Val Gly Val 
                165                 170                 175     


Leu Asp Ser Glu Tyr His Arg Ile Ala His Leu Lys Glu Arg Val Ile 
            180                 185                 190         


Glu His Ser Ile Lys Gln Ile Asn Glu His Thr Asp Ile Thr Ala Thr 
        195                 200                 205             


Tyr Glu Gln His Lys Lys Gly Arg Thr Ile Thr Gly Phe Ser Phe Lys 
    210                 215                 220                 


Phe Lys Gln Lys Lys Pro Lys Gln Ala Glu Ile Ala Thr Glu Thr Pro 
225                 230                 235                 240 


Lys Thr Ala Thr Asn Asp Pro Asp Thr Thr Lys Pro Leu Thr Glu Pro 
                245                 250                 255     


Gln Ile Ala Lys Tyr Ser Met Ile Leu Cys Lys Leu Gly Ser Ile Ser 
            260                 265                 270         


Asp Leu Ser Asn Phe Pro Asp Tyr Pro Ala Phe Ala Asn Trp Ile Gly 
        275                 280                 285             


Asn Ile Leu Arg Asn Pro Glu Lys Ala Asp Glu Gln Ile Ala Lys Arg 
    290                 295                 300                 


Ile Phe Thr Ala Leu Lys Thr Glu Thr Asp Tyr Ser Lys Lys Asn 
305                 310                 315                 


<210>  9
<211>  7
<212>  PRT
<213>  artificial sequence

<220>
<223>  MSBI1 epitope

<400>  9

Asn Arg Leu Ser Asp Arg Phe 
1               5           


<210>  10
<211>  326
<212>  PRT
<213>  artificial

<220>
<223>  CMI1.252

<400>  10

Met Ser Asp Leu Ile Val Lys Asp Asn Ala Leu Met Asn Ala Ser Tyr 
1               5                   10                  15      


Asn Leu Ala Leu Val Glu Gln Arg Leu Ile Leu Leu Ala Ile Leu Glu 
            20                  25                  30          


Ala Arg Glu Thr Gly Lys Gly Ile Asn Ala Asn Asp Pro Leu Thr Val 
        35                  40                  45              


His Ala Ser Ser Tyr Ile Asn Gln Phe Asn Val Glu Arg His Thr Ala 
    50                  55                  60                  


Tyr Gln Ala Leu Lys Asp Ala Cys Lys Asp Leu Phe Ala Arg Gln Phe 
65                  70                  75                  80  


Ser Tyr Gln Glu Lys Arg Glu Arg Gly Arg Ile Asn Ile Thr Ser Arg 
                85                  90                  95      


Trp Val Ser Gln Ile Gly Tyr Met Asp Asp Thr Ala Thr Val Glu Ile 
            100                 105                 110         


Ile Phe Ala Pro Ala Val Val Pro Leu Ile Thr Arg Leu Glu Glu Gln 
        115                 120                 125             


Phe Thr Gln Tyr Asp Ile Glu Gln Ile Ser Glu Leu Ser Ser Ala Tyr 
    130                 135                 140                 


Ala Val Arg Leu Tyr Glu Leu Leu Ile Cys Trp Arg Ser Thr Gly Lys 
145                 150                 155                 160 


Thr Pro Ile Ile Asp Leu Thr Glu Phe Arg Lys Arg Leu Gly Val Leu 
                165                 170                 175     


Asp Thr Glu Tyr Thr Arg Thr Asp Asn Leu Lys Met Arg Val Ile Glu 
            180                 185                 190         


Leu Gly Leu Lys Gln Ile Asn Glu His Thr Asp Ile Thr Ala Ser Tyr 
        195                 200                 205             


Glu Gln His Lys Lys Gly Arg Thr Ile Thr Gly Phe Ser Phe Lys Phe 
    210                 215                 220                 


Lys Gln Lys Lys Lys Thr Gly Ala Glu Met Pro Lys Asn Ser Asp Ser 
225                 230                 235                 240 


Ser Pro His Ile Glu Lys Pro Ser Gln Ile Pro Ala Asn Ile Ala Lys 
                245                 250                 255     


Gln Pro Glu Asn Ala Lys Lys Asp Asp Leu Gly His Arg Ala Ser Lys 
            260                 265                 270         


Ile Thr Gly Leu Ile Met Ser Asn Gly Leu Ala Asp Arg Phe Lys Arg 
        275                 280                 285             


Gly Asp Glu Ser Val Ile Asp Met Met Lys Arg Ile Lys Glu Glu Ile 
    290                 295                 300                 


Thr Thr Asp Thr Thr Ala Asp Gln Trp Glu Asn Lys Leu Glu Glu Phe 
305                 310                 315                 320 


Gly Val Ile Phe Gln Ser 
                325     


<210>  11
<211>  324
<212>  PRT
<213>  artificial

<220>
<223>  CMI2.214

<400>  11

Met Ser Asp Leu Ile Val Lys Asp Asn Ala Leu Met Asn Ala Ser Tyr 
1               5                   10                  15      


Asn Leu Asp Leu Val Glu Gln Arg Leu Ile Leu Leu Ala Ile Leu Glu 
            20                  25                  30          


Ala Arg Glu Thr Gly Lys Gly Ile Asn Ala Asn Asp Pro Leu Thr Val 
        35                  40                  45              


His Ala Glu Ser Tyr Ile Asn Gln Phe Gly Val Ala Arg Gln Thr Ala 
    50                  55                  60                  


Tyr Gln Ala Leu Lys Asp Ala Cys Lys Asp Leu Phe Ala Arg Gln Phe 
65                  70                  75                  80  


Ser Tyr Gln Glu Lys Arg Glu Arg Gly Arg Ala Asn Ile Thr Ser Arg 
                85                  90                  95      


Trp Val Ser Gln Ile Ala Tyr Ile Asp Glu Thr Ala Thr Val Glu Val 
            100                 105                 110         


Ile Phe Ala Pro Ala Val Val Pro Leu Ile Thr Arg Leu Glu Glu Gln 
        115                 120                 125             


Phe Thr Gln Tyr Asp Ile Glu Gln Ile Ser Gly Leu Ser Ser Ala Tyr 
    130                 135                 140                 


Ala Val Arg Leu Tyr Glu Leu Leu Ile Cys Trp Arg Ser Thr Gly Lys 
145                 150                 155                 160 


Thr Pro Val Ile Glu Leu Ala Glu Phe Arg Lys Arg Leu Gly Val Leu 
                165                 170                 175     


Asn Asp Glu Tyr Thr Arg Ser Asp Asn Phe Lys Lys Trp Ile Ile Glu 
            180                 185                 190         


Asn Pro Ile Lys Gln Ile Asn Glu His Thr Asp Ile Thr Ala Ser Tyr 
        195                 200                 205             


Glu Gln His Lys Lys Gly Arg Thr Ile Thr Gly Phe Ser Phe Lys Phe 
    210                 215                 220                 


Lys Gln Lys Lys Lys Thr Glu Pro Glu Thr Pro Lys Asn Ser Asp Ser 
225                 230                 235                 240 


Ser Gln Arg Ile Glu Lys Pro Ser Gln Ile Pro Ala Asn Ile Val Lys 
                245                 250                 255     


Gln Pro Glu Asn Ala Asn Leu Ser Asp Leu Gln His Arg Ala Ser Lys 
            260                 265                 270         


Ile Thr Gly Leu Ile Met Ser Asn Arg Leu Ser Asp Arg Phe Lys Gln 
        275                 280                 285             


Gly Asp Glu Ser Ile Met Gln Met Met Ala Arg Ile Gln Ser Glu Ile 
    290                 295                 300                 


Thr Thr Asp Ser Ile Ala Asp Gln Trp Gln Ser Lys Leu Glu Glu Phe 
305                 310                 315                 320 


Gly Val Val Phe 
                


<210>  12
<211>  324
<212>  PRT
<213>  artificial

<220>
<223>  CMI3.168

<400>  12

Met Ser Asp Leu Ile Val Lys Asp Asn Ala Leu Met Asn Ala Ser Tyr 
1               5                   10                  15      


Asn Leu Ala Leu Val Glu Gln Arg Leu Ile Leu Leu Ala Ile Leu Glu 
            20                  25                  30          


Ala Arg Glu Thr Gly Lys Gly Ile Asn Ala Asn Asp Pro Leu Thr Val 
        35                  40                  45              


His Ala Ser Ser Tyr Ile Asn Gln Phe Asn Val Glu Arg His Thr Ala 
    50                  55                  60                  


Tyr Gln Ala Leu Lys Asp Ala Cys Lys Asp Leu Phe Ala Arg Gln Phe 
65                  70                  75                  80  


Ser Tyr Gln Glu Lys Arg Glu Arg Gly Arg Ala Asn Ile Thr Ser Arg 
                85                  90                  95      


Trp Val Ser Gln Ile Ala Tyr Ile Asp Glu Thr Ala Thr Val Glu Val 
            100                 105                 110         


Ile Phe Ala Pro Ala Val Val Pro Leu Ile Thr Arg Leu Glu Glu Gln 
        115                 120                 125             


Phe Thr Gln Tyr Asp Ile Glu Gln Ile Ser Gly Leu Ser Ser Ala Tyr 
    130                 135                 140                 


Ala Val Arg Leu Tyr Glu Leu Leu Ile Cys Trp Arg Thr Thr Gly Lys 
145                 150                 155                 160 


Thr Pro Val Leu Asp Leu Thr Glu Phe Arg Lys Arg Leu Gly Val Leu 
                165                 170                 175     


Asp Thr Glu Tyr Thr Arg Thr Asp Asn Leu Lys Met Arg Val Ile Glu 
            180                 185                 190         


Gln Ser Leu Lys Gln Ile Asn Lys His Thr Asp Ile Thr Ala Ser Tyr 
        195                 200                 205             


Glu Gln His Lys Lys Gly Arg Thr Ile Thr Gly Phe Ser Phe Lys Phe 
    210                 215                 220                 


Lys Gln Lys Lys Lys Thr Glu Pro Glu Thr Pro Lys Asn Asn Asp Ser 
225                 230                 235                 240 


Gly Val Ser Lys Pro Lys Thr Val Glu Ile Pro Ala Glu Val Val Lys 
                245                 250                 255     


Gln Pro Lys Asn Thr Asn Leu Ser Asp Leu Glu Lys Arg Val Arg Met 
            260                 265                 270         


Ile Thr Gly Ala Ile Ala Lys Asn Asn Leu Ala Ser Arg Phe Gln His 
        275                 280                 285             


Gly Asn Glu Ser Pro Leu Asp Met Met Lys Arg Ile Gln Ser Glu Ile 
    290                 295                 300                 


Thr Ser Asp Glu Thr Ala Asp Leu Trp Gln Asn Lys Leu Glu Ser Met 
305                 310                 315                 320 


Gly Val Val Phe 
                


<210>  13
<211>  999
<212>  DNA
<213>  artificial sequence

<220>
<223>  codon-optimized MSBI1

<400>  13
atgagcgacc tgatcgtgaa agacaatgcc ctgatgaacg cctcctacaa cctggcactg       60

gtcgaacaga gactgattct gctggctatc atcgaggcaa gggagaccgg caagggcatc      120

aacgccaatg accccctgac agtgcacgcc agctcctaca tcaaccagtt taatgtggag      180

cgccacaccg cctatcaggc cctgaaggac gcctgcaagg atctgtttgc ccggcagttc      240

agctaccagg agaagcggga gagaggcagg atcaacatca caagcagatg ggtgtcccag      300

atcggctata tggacgatac cgccacagtg gagatcatct ttgcaccagc agtggtgcct      360

ctgatcacca ggctggagga gcagttcaca cagtacgaca tcgagcagat ctccggactg      420

tctagcgcct acgccgtgcg catgtatgag ctgctgatct gttggcggtc taccggcaag      480

acacctatca tcgagctgga tgagttccgc aagcggatcg gcgtgctgga caccgagtac      540

accagaacag ataacctgaa gatgagagtg atcgagctgg ccctgaagca gatcaatgag      600

cacaccgata tcacagcctc ttatgagcag cacaagaagg gccgcgtgat caccggcttc      660

agctttaagt tcaagcacaa gaagcagaac tctgacaaga caccaaagaa tagcgattcc      720

tctccccgga tcgtgaagca cagccagatc cctaccaaca tcgtgaagca gccagagaat      780

gccaagatgt ccgacctgga gcacagggca tctagggtga caggcgagat catgagaaat      840

aggctgagcg atcggttcaa gcagggcgac gagtccgcca tcgatatgat gaagagaatc      900

cagtccgaga tcatcaccga cgccatcgcc gatcagtggg aatctaaact ggaagagttt      960

ggagtcgtgt ttggagcaca tcaccatcat catcactga                             999


<210>  14
<211>  326
<212>  PRT
<213>  artificial sequence

<220>
<223>  MSBI1 protein

<400>  14

Met Ser Asp Leu Ile Val Lys Asp Asn Ala Leu Met Asn Ala Ser Tyr 
1               5                   10                  15      


Asn Leu Ala Leu Val Glu Gln Arg Leu Ile Leu Leu Ala Ile Ile Glu 
            20                  25                  30          


Ala Arg Glu Thr Gly Lys Gly Ile Asn Ala Asn Asp Pro Leu Thr Val 
        35                  40                  45              


His Ala Ser Ser Tyr Ile Asn Gln Phe Asn Val Glu Arg His Thr Ala 
    50                  55                  60                  


Tyr Gln Ala Leu Lys Asp Ala Cys Lys Asp Leu Phe Ala Arg Gln Phe 
65                  70                  75                  80  


Ser Tyr Gln Glu Lys Arg Glu Arg Gly Arg Ile Asn Ile Thr Ser Arg 
                85                  90                  95      


Trp Val Ser Gln Ile Gly Tyr Met Asp Asp Thr Ala Thr Val Glu Ile 
            100                 105                 110         


Ile Phe Ala Pro Ala Val Val Pro Leu Ile Thr Arg Leu Glu Glu Gln 
        115                 120                 125             


Phe Thr Gln Tyr Asp Ile Glu Gln Ile Ser Gly Leu Ser Ser Ala Tyr 
    130                 135                 140                 


Ala Val Arg Met Tyr Glu Leu Leu Ile Cys Trp Arg Ser Thr Gly Lys 
145                 150                 155                 160 


Thr Pro Ile Ile Glu Leu Asp Glu Phe Arg Lys Arg Ile Gly Val Leu 
                165                 170                 175     


Asp Thr Glu Tyr Thr Arg Thr Asp Asn Leu Lys Met Arg Val Ile Glu 
            180                 185                 190         


Leu Ala Leu Lys Gln Ile Asn Glu His Thr Asp Ile Thr Ala Ser Tyr 
        195                 200                 205             


Glu Gln His Lys Lys Gly Arg Val Ile Thr Gly Phe Ser Phe Lys Phe 
    210                 215                 220                 


Lys His Lys Lys Gln Asn Ser Asp Lys Thr Pro Lys Asn Ser Asp Ser 
225                 230                 235                 240 


Ser Pro Arg Ile Val Lys His Ser Gln Ile Pro Thr Asn Ile Val Lys 
                245                 250                 255     


Gln Pro Glu Asn Ala Lys Met Ser Asp Leu Glu His Arg Ala Ser Arg 
            260                 265                 270         


Val Thr Gly Glu Ile Met Arg Asn Arg Leu Ser Asp Arg Phe Lys Gln 
        275                 280                 285             


Gly Asp Glu Ser Ala Ile Asp Met Met Lys Arg Ile Gln Ser Glu Ile 
    290                 295                 300                 


Ile Thr Asp Ala Ile Ala Asp Gln Trp Glu Ser Lys Leu Glu Glu Phe 
305                 310                 315                 320 


Gly Val Val Phe Gly Ala 
                325     


<210>  15
<211>  974
<212>  DNA
<213>  artificial sequence

<220>
<223>  MSBI1 wild type

<400>  15
atgagcgatt taatagtaaa agataacgcc ctaatgaatg ctagttataa cttagctttg       60

gttgaacaga ggttaattct attagcaatc atagaagcga gagaaacagg caaagggatt      120

aatgccaatg atcctttaac agttcatgca agtagctata tcaatcaatt taacgtagaa      180

aggcatacgg catatcaagc cctcaaagat gcttgtaaag acttgtttgc ccgtcaattc      240

agttaccaag aaaagcgaga acgaggacga attaatatta caagtcgatg ggtttcgcaa      300

attggctata tggacgatac agcaaccgtt gagattattt ttgcccctgc ggttgttcct      360

ctgattacac ggctagagga acagttcacc cagtacgata ttgagcaaat tagcggttta      420

tcgagtgcat atgctgttcg tatgtacgaa ctgctgattt gttggcgtag cacaggcaaa      480

acaccaatta ttgagctaga cgagtttaga aagcgaatag gtgttttaga tactgaatac      540

actagaacag ataatttaaa gatgcgagtt attgaattag ccctaaaaca aatcaacgaa      600

catacagaca tcacagcaag ctatgaacaa cacaaaaaag ggcgagtgat tacaggattc      660

tcattcaagt ttaagcacaa gaaacaaaac agcgataaaa cgccaaaaaa tagcgattct      720

agcccacgta tcgtaaaaca tagtcaaatc cctccaacat tgtaaaacag cctgaaaacg      780

ccaaaatgag cgatttagaa catagagcga gccgtgttac aggggaaata atgcgaaatc      840

gtctgtcaga tcggtttaaa caaggcgatg aatcagcaat cgacatgatg aaacgtattc      900

aaagtgaaat aataaccgat gcaatagcag accagtggga aagcaaactg gaggagtttg      960

gcgtggtttt ttag                                                        974


