                         SEQUENCE LISTING

<110>  European Molecular Biology Laboratory
       Julius-Maximilians-Universitt Wrzburg	
 
<120>  CELL PENETRATING TRANSPOSASE

<130>  E31996WO

<150>  EP19158066.1
<151>  2019-02-19

<160>  3     

<170>  PatentIn version 3.5

<210>  1
<211>  340
<212>  PRT
<213>  artificial

<220>
<223>  high solubility transposase

<400>  1

Met Gly Lys Ser Lys Glu Ile Ser Gln Asp Leu Arg Lys Arg Ile Val 
1               5                   10                  15      


Asp Leu His Lys Ser Gly Ser Ser Leu Gly Ala Ile Ser Lys Arg Leu 
            20                  25                  30          


Ala Val Pro Arg Ser Ser Val Gln Thr Ile Val Arg Lys Tyr Lys His 
        35                  40                  45              


His Gly Thr Thr Gln Pro Ser Tyr Arg Ser Gly Arg Arg Arg Val Leu 
    50                  55                  60                  


Ser Pro Arg Asp Glu Arg Thr Leu Val Arg Lys Val Gln Ile Asn Pro 
65                  70                  75                  80  


Arg Thr Thr Ala Lys Asp Leu Val Lys Met Leu Glu Glu Thr Gly Thr 
                85                  90                  95      


Lys Val Ser Ile Ser Thr Val Lys Arg Val Leu Tyr Arg His Asn Leu 
            100                 105                 110         


Lys Gly His Ser Ala Arg Lys Lys Pro Leu Leu Gln Asn Arg His Lys 
        115                 120                 125             


Lys Ala Arg Leu Arg Phe Ala Thr Ala His Gly Asp Lys Asp Arg Thr 
    130                 135                 140                 


Phe Trp Arg Asn Val Leu Trp Ser Asp Glu Thr Lys Ile Glu Leu Phe 
145                 150                 155                 160 


Gly His Asn Asp His Arg Tyr Val Trp Arg Lys Lys Gly Glu Ala Ser 
                165                 170                 175     


Lys Pro Lys Asn Thr Ile Pro Thr Val Lys His Gly Gly Gly Ser Ile 
            180                 185                 190         


Met Leu Trp Gly Cys Phe Ala Ala Gly Gly Thr Gly Ala Leu His Lys 
        195                 200                 205             


Ile Asp Gly Ser Met Asp Ala Val Gln Tyr Val Asp Ile Leu Lys Gln 
    210                 215                 220                 


His Leu Lys Thr Ser Val Arg Lys Leu Lys Leu Gly Arg Lys Trp Val 
225                 230                 235                 240 


Phe Gln His Asp Asn Asp Pro Lys His Thr Ser Lys Val Val Ala Lys 
                245                 250                 255     


Trp Leu Lys Asp Asn Lys Val Lys Val Leu Glu Trp Pro Ser Gln Ser 
            260                 265                 270         


Pro Asp Leu Asn Pro Ile Glu Asn Leu Trp Ala Glu Leu Lys Lys Arg 
        275                 280                 285             


Val Arg Ala Arg Arg Pro Thr Asn Leu Thr Gln Leu His Gln Leu Cys 
    290                 295                 300                 


Gln Glu Glu Trp Ala Lys Ile His Pro Asn Tyr Cys Gly Lys Leu Val 
305                 310                 315                 320 


Glu Gly Tyr Pro Lys Arg Leu Thr Gln Val Lys Gln Phe Lys Gly Asn 
                325                 330                 335     


Ala Thr Lys Tyr 
            340 


<210>  2
<211>  340
<212>  PRT
<213>  artificial

<220>
<223>  SB100X

<400>  2

Met Gly Lys Ser Lys Glu Ile Ser Gln Asp Leu Arg Lys Arg Ile Val 
1               5                   10                  15      


Asp Leu His Lys Ser Gly Ser Ser Leu Gly Ala Ile Ser Lys Arg Leu 
            20                  25                  30          


Ala Val Pro Arg Ser Ser Val Gln Thr Ile Val Arg Lys Tyr Lys His 
        35                  40                  45              


His Gly Thr Thr Gln Pro Ser Tyr Arg Ser Gly Arg Arg Arg Val Leu 
    50                  55                  60                  


Ser Pro Arg Asp Glu Arg Thr Leu Val Arg Lys Val Gln Ile Asn Pro 
65                  70                  75                  80  


Arg Thr Thr Ala Lys Asp Leu Val Lys Met Leu Glu Glu Thr Gly Thr 
                85                  90                  95      


Lys Val Ser Ile Ser Thr Val Lys Arg Val Leu Tyr Arg His Asn Leu 
            100                 105                 110         


Lys Gly His Ser Ala Arg Lys Lys Pro Leu Leu Gln Asn Arg His Lys 
        115                 120                 125             


Lys Ala Arg Leu Arg Phe Ala Thr Ala His Gly Asp Lys Asp Arg Thr 
    130                 135                 140                 


Phe Trp Arg Asn Val Leu Trp Ser Asp Glu Thr Lys Ile Glu Leu Phe 
145                 150                 155                 160 


Gly His Asn Asp His Arg Tyr Val Trp Arg Lys Lys Gly Glu Ala Cys 
                165                 170                 175     


Lys Pro Lys Asn Thr Ile Pro Thr Val Lys His Gly Gly Gly Ser Ile 
            180                 185                 190         


Met Leu Trp Gly Cys Phe Ala Ala Gly Gly Thr Gly Ala Leu His Lys 
        195                 200                 205             


Ile Asp Gly Ile Met Asp Ala Val Gln Tyr Val Asp Ile Leu Lys Gln 
    210                 215                 220                 


His Leu Lys Thr Ser Val Arg Lys Leu Lys Leu Gly Arg Lys Trp Val 
225                 230                 235                 240 


Phe Gln His Asp Asn Asp Pro Lys His Thr Ser Lys Val Val Ala Lys 
                245                 250                 255     


Trp Leu Lys Asp Asn Lys Val Lys Val Leu Glu Trp Pro Ser Gln Ser 
            260                 265                 270         


Pro Asp Leu Asn Pro Ile Glu Asn Leu Trp Ala Glu Leu Lys Lys Arg 
        275                 280                 285             


Val Arg Ala Arg Arg Pro Thr Asn Leu Thr Gln Leu His Gln Leu Cys 
    290                 295                 300                 


Gln Glu Glu Trp Ala Lys Ile His Pro Asn Tyr Cys Gly Lys Leu Val 
305                 310                 315                 320 


Glu Gly Tyr Pro Lys Arg Leu Thr Gln Val Lys Gln Phe Lys Gly Asn 
                325                 330                 335     


Ala Thr Lys Tyr 
            340 


<210>  3
<211>  343
<212>  PRT
<213>  artificial

<220>
<223>  hsSB for recombinant expression

<400>  3

Gly Pro Met Met Gly Lys Ser Lys Glu Ile Ser Gln Asp Leu Arg Lys 
1               5                   10                  15      


Arg Ile Val Asp Leu His Lys Ser Gly Ser Ser Leu Gly Ala Ile Ser 
            20                  25                  30          


Lys Arg Leu Ala Val Pro Arg Ser Ser Val Gln Thr Ile Val Arg Lys 
        35                  40                  45              


Tyr Lys His His Gly Thr Thr Gln Pro Ser Tyr Arg Ser Gly Arg Arg 
    50                  55                  60                  


Arg Val Leu Ser Pro Arg Asp Glu Arg Thr Leu Val Arg Lys Val Gln 
65                  70                  75                  80  


Ile Asn Pro Arg Thr Thr Ala Lys Asp Leu Val Lys Met Leu Glu Glu 
                85                  90                  95      


Thr Gly Thr Lys Val Ser Ile Ser Thr Val Lys Arg Val Leu Tyr Arg 
            100                 105                 110         


His Asn Leu Lys Gly His Ser Ala Arg Lys Lys Pro Leu Leu Gln Asn 
        115                 120                 125             


Arg His Lys Lys Ala Arg Leu Arg Phe Ala Thr Ala His Gly Asp Lys 
    130                 135                 140                 


Asp Arg Thr Phe Trp Arg Asn Val Leu Trp Ser Asp Glu Thr Lys Ile 
145                 150                 155                 160 


Glu Leu Phe Gly His Asn Asp His Arg Tyr Val Trp Arg Lys Lys Gly 
                165                 170                 175     


Glu Ala Ser Lys Pro Lys Asn Thr Ile Pro Thr Val Lys His Gly Gly 
            180                 185                 190         


Gly Ser Ile Met Leu Trp Gly Cys Phe Ala Ala Gly Gly Thr Gly Ala 
        195                 200                 205             


Leu His Lys Ile Asp Gly Ser Met Asp Ala Val Gln Tyr Val Asp Ile 
    210                 215                 220                 


Leu Lys Gln His Leu Lys Thr Ser Val Arg Lys Leu Lys Leu Gly Arg 
225                 230                 235                 240 


Lys Trp Val Phe Gln His Asp Asn Asp Pro Lys His Thr Ser Lys Val 
                245                 250                 255     


Val Ala Lys Trp Leu Lys Asp Asn Lys Val Lys Val Leu Glu Trp Pro 
            260                 265                 270         


Ser Gln Ser Pro Asp Leu Asn Pro Ile Glu Asn Leu Trp Ala Glu Leu 
        275                 280                 285             


Lys Lys Arg Val Arg Ala Arg Arg Pro Thr Asn Leu Thr Gln Leu His 
    290                 295                 300                 


Gln Leu Cys Gln Glu Glu Trp Ala Lys Ile His Pro Asn Tyr Cys Gly 
305                 310                 315                 320 


Lys Leu Val Glu Gly Tyr Pro Lys Arg Leu Thr Gln Val Lys Gln Phe 
                325                 330                 335     


Lys Gly Asn Ala Thr Lys Tyr 
            340             


