                         SEQUENCE LISTING



<110>  IRTA, Institut de Recerca i Transferncia Agroalimentries

 

<120>  Recombinant antimicrobial multidomain polypeptide, methods of 

       producing and uses thereof



<130>  PD10206PC00

<150>  EP19382115.4
<151>  2019-02-19



<160>  18    



<170>  PatentIn version 3.5



<210>  1

<211>  8

<212>  PRT

<213>  Artificial Sequence



<220>

<223>  Linker sequence



<400>  1



Ser Gly Gly Gly Ser Gly Gly Ser 

1               5               





<210>  2

<211>  62

<212>  PRT

<213>  Homo sapiens



<400>  2



Arg Lys Arg Met Arg Asn Arg Ile Ala Ala Ser Lys Cys Arg Lys Arg 

1               5                   10                  15      





Lys Leu Glu Arg Ile Ala Arg Leu Glu Glu Lys Val Lys Thr Leu Lys 

            20                  25                  30          





Ala Gln Asn Ser Glu Leu Ala Ser Thr Ala Asn Met Leu Arg Glu Gln 

        35                  40                  45              





Val Ala Gln Leu Lys Gln Lys Val Met Asn His Val Asn Ser 

    50                  55                  60          





<210>  3

<211>  94

<212>  PRT

<213>  Homo sapiens



<400>  3



Met Arg Thr Ile Ala Ile Leu Ala Ala Ile Leu Leu Val Ala Leu Gln 

1               5                   10                  15      





Ala Gln Ala Glu Ser Leu Gln Glu Arg Ala Asp Glu Ala Thr Thr Gln 

            20                  25                  30          





Lys Gln Ser Gly Glu Asp Asn Gln Asp Leu Ala Ile Ser Phe Ala Gly 

        35                  40                  45              





Asn Gly Leu Ser Ala Leu Arg Thr Ser Gly Ser Gln Ala Arg Ala Thr 

    50                  55                  60                  





Cys Tyr Cys Arg Thr Gly Arg Cys Ala Thr Arg Glu Ser Leu Ser Gly 

65                  70                  75                  80  





Val Cys Glu Ile Ser Gly Arg Leu Tyr Arg Leu Cys Cys Arg 

                85                  90                  





<210>  4

<211>  10

<212>  PRT

<213>  Homo sapiens



<400>  4



Gln Arg Leu Phe Gln Val Lys Gly Arg Arg 

1               5                   10  





<210>  5

<211>  189

<212>  PRT

<213>  Homo sapiens



<400>  5



Met Ala Leu Leu Ser Arg Pro Ala Leu Thr Leu Leu Leu Leu Leu Met 

1               5                   10                  15      





Ala Ala Val Val Arg Cys Gln Glu Gln Ala Gln Thr Thr Asp Trp Arg 

            20                  25                  30          





Ala Thr Leu Lys Thr Ile Arg Asn Gly Val His Lys Ile Asp Thr Tyr 

        35                  40                  45              





Leu Asn Ala Ala Leu Asp Leu Leu Gly Gly Glu Asp Gly Leu Cys Gln 

    50                  55                  60                  





Tyr Lys Cys Ser Asp Gly Ser Lys Pro Phe Pro Arg Tyr Gly Tyr Lys 

65                  70                  75                  80  





Pro Ser Pro Pro Asn Gly Cys Gly Ser Pro Leu Phe Gly Val His Leu 

                85                  90                  95      





Asn Ile Gly Ile Pro Ser Leu Thr Lys Cys Cys Asn Gln His Asp Arg 

            100                 105                 110         





Cys Tyr Glu Thr Cys Gly Lys Ser Lys Asn Asp Cys Asp Glu Glu Phe 

        115                 120                 125             





Gln Tyr Cys Leu Ser Lys Ile Cys Arg Asp Val Gln Lys Thr Leu Gly 

    130                 135                 140                 





Leu Thr Gln His Val Gln Ala Cys Glu Thr Thr Val Glu Leu Leu Phe 

145                 150                 155                 160 





Asp Ser Val Ile His Leu Gly Cys Lys Pro Tyr Leu Asp Ser Gln Arg 

                165                 170                 175     





Ala Ala Cys Arg Cys His Tyr Glu Glu Lys Thr Asp Leu 

            180                 185                 





<210>  6

<211>  93

<212>  PRT

<213>  Homo sapiens



<400>  6



Gly Arg Ala Gln Ser Ile Gly Arg Arg Gly Lys Val Glu Gln Leu Ser 

1               5                   10                  15      





Pro Glu Glu Glu Glu Lys Arg Arg Ile Arg Arg Glu Arg Asn Lys Met 

            20                  25                  30          





Ala Ala Ala Lys Cys Arg Asn Arg Arg Arg Glu Leu Thr Asp Thr Leu 

        35                  40                  45              





Gln Ala Glu Thr Asp Gln Leu Glu Asp Glu Lys Ser Ala Leu Gln Thr 

    50                  55                  60                  





Glu Ile Ala Asn Leu Leu Lys Glu Lys Glu Lys Leu Glu Phe Ile Leu 

65                  70                  75                  80  





Ala Ala His Arg Pro Ala Cys Lys Ile Pro Asp Asp Leu 

                85                  90              





<210>  7

<211>  6

<212>  PRT

<213>  Artificial Sequence



<220>

<223>  His Tag H6



<400>  7



His His His His His His 

1               5       





<210>  8

<211>  32

<212>  PRT

<213>  Homo sapiens



<400>  8



Ala Thr Cys Tyr Cys Arg Thr Gly Arg Cys Ala Thr Arg Glu Ser Leu 

1               5                   10                  15      





Ser Gly Val Cys Glu Ile Ser Gly Arg Leu Tyr Arg Leu Cys Cys Arg 

            20                  25                  30          





<210>  9

<211>  167

<212>  PRT

<213>  Homo sapiens



<400>  9



Gln Glu Gln Ala Gln Thr Thr Asp Trp Arg Ala Thr Leu Lys Thr Ile 

1               5                   10                  15      





Arg Asn Gly Val His Lys Ile Asp Thr Tyr Leu Asn Ala Ala Leu Asp 

            20                  25                  30          





Leu Leu Gly Gly Glu Asp Gly Leu Cys Gln Tyr Lys Cys Ser Asp Gly 

        35                  40                  45              





Ser Lys Pro Phe Pro Arg Tyr Gly Tyr Lys Pro Ser Pro Pro Asn Gly 

    50                  55                  60                  





Cys Gly Ser Pro Leu Phe Gly Val His Leu Asn Ile Gly Ile Pro Ser 

65                  70                  75                  80  





Leu Thr Lys Cys Cys Asn Gln His Asp Arg Cys Tyr Glu Thr Cys Gly 

                85                  90                  95      





Lys Ser Lys Asn Asp Cys Asp Glu Glu Phe Gln Tyr Cys Leu Ser Lys 

            100                 105                 110         





Ile Cys Arg Asp Val Gln Lys Thr Leu Gly Leu Thr Gln His Val Gln 

        115                 120                 125             





Ala Cys Glu Thr Thr Val Glu Leu Leu Phe Asp Ser Val Ile His Leu 

    130                 135                 140                 





Gly Cys Lys Pro Tyr Leu Asp Ser Gln Arg Ala Ala Cys Arg Cys His 

145                 150                 155                 160 





Tyr Glu Glu Lys Thr Asp Leu 

                165         





<210>  10

<211>  486

<212>  PRT

<213>  Artificial Sequence



<220>

<223>  JAMF1



<400>  10



Arg Lys Arg Met Arg Asn Arg Ile Ala Ala Ser Lys Cys Arg Lys Arg 

1               5                   10                  15      





Lys Leu Glu Arg Ile Ala Arg Leu Glu Glu Lys Val Lys Thr Leu Lys 

            20                  25                  30          





Ala Gln Asn Ser Glu Leu Ala Ser Thr Ala Asn Met Leu Arg Glu Gln 

        35                  40                  45              





Val Ala Gln Leu Lys Gln Lys Val Met Asn His Val Asn Ser Ser Gly 

    50                  55                  60                  





Gly Gly Ser Gly Gly Ser Met Arg Thr Ile Ala Ile Leu Ala Ala Ile 

65                  70                  75                  80  





Leu Leu Val Ala Leu Gln Ala Gln Ala Glu Ser Leu Gln Glu Arg Ala 

                85                  90                  95      





Asp Glu Ala Thr Thr Gln Lys Gln Ser Gly Glu Asp Asn Gln Asp Leu 

            100                 105                 110         





Ala Ile Ser Phe Ala Gly Asn Gly Leu Ser Ala Leu Arg Thr Ser Gly 

        115                 120                 125             





Ser Gln Ala Arg Ala Thr Cys Tyr Cys Arg Thr Gly Arg Cys Ala Thr 

    130                 135                 140                 





Arg Glu Ser Leu Ser Gly Val Cys Glu Ile Ser Gly Arg Leu Tyr Arg 

145                 150                 155                 160 





Leu Cys Cys Arg Ser Gly Gly Gly Ser Gly Gly Ser Gln Arg Leu Phe 

                165                 170                 175     





Gln Val Lys Gly Arg Arg Ser Gly Gly Gly Ser Gly Gly Ser Met Ala 

            180                 185                 190         





Leu Leu Ser Arg Pro Ala Leu Thr Leu Leu Leu Leu Leu Met Ala Ala 

        195                 200                 205             





Val Val Arg Cys Gln Glu Gln Ala Gln Thr Thr Asp Trp Arg Ala Thr 

    210                 215                 220                 





Leu Lys Thr Ile Arg Asn Gly Val His Lys Ile Asp Thr Tyr Leu Asn 

225                 230                 235                 240 





Ala Ala Leu Asp Leu Leu Gly Gly Glu Asp Gly Leu Cys Gln Tyr Lys 

                245                 250                 255     





Cys Ser Asp Gly Ser Lys Pro Phe Pro Arg Tyr Gly Tyr Lys Pro Ser 

            260                 265                 270         





Pro Pro Asn Gly Cys Gly Ser Pro Leu Phe Gly Val His Leu Asn Ile 

        275                 280                 285             





Gly Ile Pro Ser Leu Thr Lys Cys Cys Asn Gln His Asp Arg Cys Tyr 

    290                 295                 300                 





Glu Thr Cys Gly Lys Ser Lys Asn Asp Cys Asp Glu Glu Phe Gln Tyr 

305                 310                 315                 320 





Cys Leu Ser Lys Ile Cys Arg Asp Val Gln Lys Thr Leu Gly Leu Thr 

                325                 330                 335     





Gln His Val Gln Ala Cys Glu Thr Thr Val Glu Leu Leu Phe Asp Ser 

            340                 345                 350         





Val Ile His Leu Gly Cys Lys Pro Tyr Leu Asp Ser Gln Arg Ala Ala 

        355                 360                 365             





Cys Arg Cys His Tyr Glu Glu Lys Thr Asp Leu Ser Gly Gly Gly Ser 

    370                 375                 380                 





Gly Gly Ser Gly Arg Ala Gln Ser Ile Gly Arg Arg Gly Lys Val Glu 

385                 390                 395                 400 





Gln Leu Ser Pro Glu Glu Glu Glu Lys Arg Arg Ile Arg Arg Glu Arg 

                405                 410                 415     





Asn Lys Met Ala Ala Ala Lys Cys Arg Asn Arg Arg Arg Glu Leu Thr 

            420                 425                 430         





Asp Thr Leu Gln Ala Glu Thr Asp Gln Leu Glu Asp Glu Lys Ser Ala 

        435                 440                 445             





Leu Gln Thr Glu Ile Ala Asn Leu Leu Lys Glu Lys Glu Lys Leu Glu 

    450                 455                 460                 





Phe Ile Leu Ala Ala His Arg Pro Ala Cys Lys Ile Pro Asp Asp Leu 

465                 470                 475                 480 





His His His His His His 

                485     





<210>  11

<211>  402

<212>  PRT

<213>  Artificial Sequence



<220>

<223>  JAMF2



<400>  11



Arg Lys Arg Met Arg Asn Arg Ile Ala Ala Ser Lys Cys Arg Lys Arg 

1               5                   10                  15      





Lys Leu Glu Arg Ile Ala Arg Leu Glu Glu Lys Val Lys Thr Leu Lys 

            20                  25                  30          





Ala Gln Asn Ser Glu Leu Ala Ser Thr Ala Asn Met Leu Arg Glu Gln 

        35                  40                  45              





Val Ala Gln Leu Lys Gln Lys Val Met Asn His Val Asn Ser Ser Gly 

    50                  55                  60                  





Gly Gly Ser Gly Gly Ser Ala Thr Cys Tyr Cys Arg Thr Gly Arg Cys 

65                  70                  75                  80  





Ala Thr Arg Glu Ser Leu Ser Gly Val Cys Glu Ile Ser Gly Arg Leu 

                85                  90                  95      





Tyr Arg Leu Cys Cys Arg Ser Gly Gly Gly Ser Gly Gly Ser Gln Arg 

            100                 105                 110         





Leu Phe Gln Val Lys Gly Arg Arg Ser Gly Gly Gly Ser Gly Gly Ser 

        115                 120                 125             





Gln Glu Gln Ala Gln Thr Thr Asp Trp Arg Ala Thr Leu Lys Thr Ile 

    130                 135                 140                 





Arg Asn Gly Val His Lys Ile Asp Thr Tyr Leu Asn Ala Ala Leu Asp 

145                 150                 155                 160 





Leu Leu Gly Gly Glu Asp Gly Leu Cys Gln Tyr Lys Cys Ser Asp Gly 

                165                 170                 175     





Ser Lys Pro Phe Pro Arg Tyr Gly Tyr Lys Pro Ser Pro Pro Asn Gly 

            180                 185                 190         





Cys Gly Ser Pro Leu Phe Gly Val His Leu Asn Ile Gly Ile Pro Ser 

        195                 200                 205             





Leu Thr Lys Cys Cys Asn Gln His Asp Arg Cys Tyr Glu Thr Cys Gly 

    210                 215                 220                 





Lys Ser Lys Asn Asp Cys Asp Glu Glu Phe Gln Tyr Cys Leu Ser Lys 

225                 230                 235                 240 





Ile Cys Arg Asp Val Gln Lys Thr Leu Gly Leu Thr Gln His Val Gln 

                245                 250                 255     





Ala Cys Glu Thr Thr Val Glu Leu Leu Phe Asp Ser Val Ile His Leu 

            260                 265                 270         





Gly Cys Lys Pro Tyr Leu Asp Ser Gln Arg Ala Ala Cys Arg Cys His 

        275                 280                 285             





Tyr Glu Glu Lys Thr Asp Leu Ser Gly Gly Gly Ser Gly Gly Ser Gly 

    290                 295                 300                 





Arg Ala Gln Ser Ile Gly Arg Arg Gly Lys Val Glu Gln Leu Ser Pro 

305                 310                 315                 320 





Glu Glu Glu Glu Lys Arg Arg Ile Arg Arg Glu Arg Asn Lys Met Ala 

                325                 330                 335     





Ala Ala Lys Cys Arg Asn Arg Arg Arg Glu Leu Thr Asp Thr Leu Gln 

            340                 345                 350         





Ala Glu Thr Asp Gln Leu Glu Asp Glu Lys Ser Ala Leu Gln Thr Glu 

        355                 360                 365             





Ile Ala Asn Leu Leu Lys Glu Lys Glu Lys Leu Glu Phe Ile Leu Ala 

    370                 375                 380                 





Ala His Arg Pro Ala Cys Lys Ile Pro Asp Asp Leu His His His His 

385                 390                 395                 400 





His His 

        





<210>  12

<211>  8

<212>  PRT

<213>  Artificial Sequence



<220>

<223>  Aggregation tag GFIL8



<400>  12



Gly Phe Ile Leu Gly Phe Ile Leu 

1               5               





<210>  13

<211>  16

<212>  PRT

<213>  Artificial Sequence



<220>

<223>  Aggregation tag  GFIL16



<400>  13



Gly Phe Ile Leu Gly Phe Ile Leu Gly Phe Ile Leu Gly Phe Ile Leu 

1               5                   10                  15      





<210>  14

<211>  18

<212>  PRT

<213>  Artificial Sequence



<220>

<223>  Aggregation tag alpha helical peptide 18A



<400>  14



Glu Trp Leu Lys Ala Phe Tyr Glu Lys Val Leu Glu Lys Leu Lys Glu 

1               5                   10                  15      





Leu Phe 

        





<210>  15

<211>  16

<212>  PRT

<213>  Artificial Sequence



<220>

<223>  Aggregation tag beta-strand peptide ELK16



<400>  15



Leu Glu Leu Glu Leu Lys Leu Lys Leu Glu Leu Glu Leu Lys Leu Lys 

1               5                   10                  15      





<210>  16

<211>  8

<212>  PRT

<213>  Artificial Sequence



<220>

<223>  Aggregation tag surfactant-like peptide L6KD



<400>  16



Leu Leu Leu Leu Leu Leu Lys Asp 

1               5               





<210>  17

<211>  8

<212>  PRT

<213>  Artificial Sequence



<220>

<223>  Aggregation tag L6K2



<400>  17



Leu Leu Leu Leu Leu Leu Lys Lys 

1               5               





<210>  18

<211>  8

<212>  PRT

<213>  Artificial Sequence



<220>

<223>  Aggregation tag DKL6



<400>  18



Asp Lys Leu Leu Leu Leu Leu Leu 

1               5               





