               SEQUENCE LISTING

<110> Synbionik Gmbh

<120> Production of plant-based active substances (e.g. cannabinoids) by recombinant microorganisms

<130> PCT

<160> 52

<170> BiSSAP 1.3.6

<210> 1
<211> 4377
<212> DNA
<213> plasmids


<220> 
<223> pSUZM2 (P1)

<400> 1
cggaaaggtc atcctgaaaa ttctattcgt attctgatca accagatcgt gaatgatcgg       60

gcgatgagcc ggaaacttgt cccgtgatca gaaccggcgc agcctcttcc cttttatcta      120

tttcaaattc aggcctgcca cagcggagaa tcttcgctgt ggcttttttg cgtccaagac      180

cggagcccgc acaatgaccg cctcttcctc ttcagcgccc ctctcggatg cctctgtctt      240

ttctttagag cctgtctctg cgtcttttga acaaacgacg cccgtaacca gtccttttat      300

cgacaggcta ggccgtttca agcgtggcac ttatcgaacc atgttggttg aaacgggtat      360

gccatttgac agcgcaaaga ttatggcaga tcaggccgca gaacaacata aaagagagcg      420

cacattctct aaattaatgc ccttggccaa tggcgctcat cccgaaattt cggcctcaaa      480

taccaatatt gaggctacag cctctgcatc agatttcgac gaaatcgaat attttgacca      540

gatggtatca aacggcgttg atcctagaga tgccgcagat attgctcgca aaacagctat      600

tgcgagacgt cgagaccctc tcgaacgagc cattcgctat aaaacccgcc gtcaaactcc      660

tgatcctgtg catcgggatt cttatgaggt gggcgaacgc gagaagtcgg tctggaagcc      720

ggtcaatccg caagagattg gtgcctatct ggaagcggtt gatcaatatt cgatcaaaac      780

caagggctta agcgataagg ccgttcgtct tctaaagatg ctgtttcgga tggtcgactt      840

taaaactggc cgtctggagc cgaccctcga cacaatatgc gatcgtgtcg gttatgcccg      900

tgcgacggtc gtcaaattac tgcggcaatt acaggattta ggcttcatcc gctggatacg      960

ccggtcaatc aagatcaagg cggacggcaa aggcccccgc cgcaagcaga ccagtaacgc     1020

ctacggcttc ttgtctccca aggcttggcc agatttggcc agacaggttt tcgagcgtgt     1080

gatgcgccgg aggcatgcgc ctattcctga cgatatcgac cacgcccaag aggcggacaa     1140

agccgaaacc aaggctatcg ttgaaaactt gccgcccgaa gatttcgtca aagcaatatc     1200

gggcgataaa gagccaacag agttaacaga ttcgttaata cagctcgcca aatctatcga     1260

gcgggcggaa caacgggaac aagaagaaca ggctaagaag gcagcacagg ccggtcagga     1320

agaggttaaa acatcttccc aaacacctaa ggaggatacc tcaaaaaagc ccccaatctc     1380

gggcaatcaa cgcgagttca attcttatac tctatcctgt tatacttatt tatctaatag     1440

cgaccataca aaactaaaaa ccaaagaaga agataaagac tgcactgctc ctgctaccgc     1500

aggcagtgca gaccctattt atgatcgaaa acctgacagc aatcctttcc tctcaactct     1560

caaagctcgc ggttatcgtc ctgatcaaat caacgcttgc ctcgaaaaca gagataaacg     1620

gaaaaagaaa ggatggatgc ctctgtcacc cgatgaaacc gcccgcctcc ttattcaaac     1680

aaaacagatg tgcccataac aacgaccact atccttctca aagcatatcg tccgttaatc     1740

tctcggcgct gctcgcttcg ctcacaggct cggcgagaaa gaaatttgcc tttcggggga     1800

ctatctaatt cctaacaatt cgcattatcg ataatgtaat aaaaggctaa tctttaaaaa     1860

ccaaattaca gcgattacat catttctatg aatgtgtatt atacataata tgagaagtcg     1920

tgatttgatc aaagaagtta aaaaagccaa tattaccagc ttgacagata taaaataggc     1980

cttggttgat ttcgtgaaag aactggaaga ttgcacaatt cttctttctg gtgatatctt     2040

tgacccgacg gccctcagtc aatgagtgtg cgccgcattt ctgcccattc aatcagggcc     2100

tgcattgaag ggccgagagc gtatcccata ccgctgagct cgtattctac ctttggcggg     2160

acctgcggat agaccgttcg gagcacgatg cgatcctttt ccagttgctt gagctgctgg     2220

atcagcatct tctggttgat gccgtcgatc tgcttttcca gtgaggagaa gcggattggg     2280

ccttttgcgg caaacaactg gcagatgata acaatcttcc attttccttc gagtaccctg     2340

agcgcttctg tcgtcgcctc aggaattcga gctcggtacc cggggatcct ctagagtcga     2400

cctgcaggca tgcaagcttg gatccataaa acgaaaggct cagtcgaaag actgggcctt     2460

tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgggagc     2520

ggatttgaac gttgcgaagc aacggcccgg agggtggcgg gcaggacgcc cgccataaac     2580

tgccaggcat caaattaagc agaaggccat cctgacggat ggcctttgta gaaaagatca     2640

aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac     2700

caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg     2760

taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag     2820

gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac     2880

cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt     2940

taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg     3000

agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc     3060

ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc     3120

gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc     3180

acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa     3240

acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatat     3300

gttcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa     3360

aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat     3420

tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc     3480

agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga     3540

gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg     3600

cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc     3660

agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag     3720

taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc     3780

tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg     3840

taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg     3900

acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac     3960

ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac     4020

cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg     4080

agcgtggttc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg     4140

tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg     4200

agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac     4260

tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg     4320

ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg cggccgc        4377


<210> 2
<211> 4753
<212> DNA
<213> plasmids


<220> 
<223> pSUZM1 (P2)

<400> 2
gcaccgcaaa ggacatgctg atcgcgagat cggcgcgcta ctcgacactg tcataacaga       60

tcacattcgg agatagctga tgaatactgc gcagaatcag actcttaaaa gagcgttttt      120

gtcatcagaa gccgcagcag ctgactcttc agctgactgc ggcttttcta tgtctgaaaa      180

agatatgcca gattttgaag aaatgacgcc agccagccct tttatcgaca ggctaggccg      240

tttcaagcgc ggcacttatc gaagcatgtt gatgcagacg gggctttctt atgaacgcgc      300

gaaaggcatg gcagacaatg cggcggaaga atttaaaaaa tcaaaagcaa agtcgaaatc      360

tttgccattg gccaagagcg aaatcgctga acttggggct tcagaagcaa cgattgaagt      420

aacagctcat caggtaacat tttctgaatc acaggcacaa ccagaacctt tcgatgctta      480

ctgctgcttg caacagttaa aagatcaggg cgtggacggt gccacagctg cgaagattgc      540

aaatcaggcc tacaagcatc acaaagaagc cgaaaatttt gacgaagcgg tatatttcaa      600

ggaattagtc gatactggca tagatgcggt gatggctgct aatatggctc gtaaaacagc      660

cgaagctaag cgccgcgacc cgctggaaag atacggtatc cgtttaaagc cgcgccgcac      720

gcctgatcct gttcaccgtg actcttatga tgttggcgag cgcgaaaata cggtctggaa      780

gccggtcaat ccgaatgaga tcggggctta tctggaagca gtcgatcaat attcaatcaa      840

gaccggcaat atcagtgata gagccgtccg gctgctcaag atgctgtttc ggatggtgga      900

cttcaagaca ggtcgattag agccgacgct tgatacaatc tgcgaatggg tcggctatgc      960

acgggcaacg gttgtccgct tgctaaaaca gcttcaagag caaggtttta tccgctggat     1020

acgccgttca atcaagatca agacggatgg ggctgggccc cgccgcaaac agaccagtaa     1080

cgcttacggt ttcttgtcgc ctaaatcttg gccggagatg gcaagaaagg ttttcgagcg     1140

cgtgatgcgc cggaagaatg cgccggtctc tgctgatctt gctcatgctc aagaagccga     1200

caaagtcgaa accggtgcca tgattgaaag ccagccgaat gacgactggg tgaaagaaat     1260

cttcgcacag aagcgcgcgc aacatccgac gagtgacgag cagaaaaaca aaaaagaaca     1320

ggccgatgat ctggcagaga ctcttgccag cttcgccaaa tctatcgagc gggcagaaca     1380

acgggaacaa gaagaacagg ccaagaaagc catttcggcc gagcaaaatg aggttaaaac     1440

accctacaaa acactcaagg aggacacctc aaaaacgccc caaatttcgg cagttgagcg     1500

cgagttcaat tttgatactc tatcccgtta tagttatttt aataataacc actccaatca     1560

atttaaaaac caaaaagaga ttaaggactg cactgctcct gctgccgcag gcagtgcaga     1620

cgttgttttg aaaacaaaac ctcaaaccga cactttccta aaaacccttc aaactcgcgg     1680

ttaccgtcaa gaccagacca acgcttgcct cgaaaacaga gataaacgga aaaagaatgg     1740

atggatgcct ctgtcagcgg acgaaaccgc ccgcctcctt atacaaacaa ggcagatgcg     1800

accgtgatac cgacagctat ccttctcaaa gcacaccgcc cattaatctc tcggcgctgc     1860

tcgcttcgct cacaggctcg gcgaaaaaga aatttgcctt ttggggggct attaacttct     1920

aacaactcgc attatcgaaa tgtctttaaa taggtttatt ctctaaaagc gtttcgacat     1980

ggcttttact gttatccatc atccaatctt cagtcggtct tgagcgatat ccaatcaaac     2040

gaacaattct atcccagtca gcttgtagat ctctttcaaa tataggctca tgatgggcta     2100

tccgattacg cagcatccga atttcaccca aatctctata gagactctgt ctctggattt     2160

taatctcttg ctgtggatca agattcggga acacgttcaa caaatagaga tcccaaagcc     2220

gtttatcatg gcgctttgtg aacatagcct gccagaatac aaaattcagt tctggtatta     2280

cctttccggt cgtttccgcc ttcaaacagg ctttgtttac atccttcgat gcgctgtatc     2340

ctgatttgac aactggaagg cttcgtcgga aaccttctga ccacggccac tgatctccat     2400

aggtatagga tagcgcttcc gccacagcat ttcgggtcgc tatttctgtt atatgaaggg     2460

gggctagcaa tgcgccggat agctgggcat tccacacata taaatccaga gcgacgagat     2520

catcttttcc caatgccctg actgtgtctt catatcttcc aagtcgggga gccgataagg     2580

tctttctgat agaggtaatc ttgtcttgaa ttgacagcat ggctacccat ttatatgatc     2640

aaccacatat tcgcctcggg tctttgttgg cttatgcctc cccctgagga caaaagatac     2700

aaaaaagacc tcgcttacgg cggggtcttt ttttatccat tttcctgtat tgtataaagt     2760

ctttgctagc ctgacggaaa acgtaatcct gcggttaatc ggaattcgag ctcggtaccc     2820

ggggatcctc tagagtcgac ctgcaggcat gcaagcttgg atccataaaa cgaaaggctc     2880

agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctcctgagta     2940

ggacaaatcc gccgggagcg gatttgaacg ttgcgaagca acggcccgga gggtggcggg     3000

caggacgccc gccataaact gccaggcatc aaattaagca gaaggccatc ctgacggatg     3060

gcctttgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc     3120

tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta     3180

ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt     3240

ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc     3300

gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg     3360

ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg     3420

tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag     3480

ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc     3540

agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat     3600

agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg     3660

gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc     3720

tggccttttg ctcacatatg ttcaaatatg tatccgctca tgagacaata accctgataa     3780

atgcttcaat aatattgaaa aaggaagagt atgattgaac aggatggcct gcatgcgggt     3840

agcccggcag cgtgggtgga acgtctgttt ggctatgatt gggcgcagca gaccattggc     3900

tgctctgatg cggcggtgtt tcgtctgagc gcgcagggtc gtccggtgct gtttgtgaaa     3960

accgatctga gcggtgcgct gaacgagctg caggatgaag cggcgcgtct gagctggctg     4020

gccaccaccg gtgttccgtg tgcggcggtg ctggatgtgg tgaccgaagc gggccgtgat     4080

tggctgctgc tgggcgaagt gccgggtcag gatctgctgt ctagccatct ggcgccggca     4140

gaaaaagtga gcattatggc ggatgccatg cgtcgtctgc ataccctgga cccggcgacc     4200

tgtccgtttg atcatcaggc gaaacatcgt attgaacgtg cgcgtacccg tatggaagcg     4260

ggcctggtgg atcaggatga tctggatgaa gaacatcagg gcctggcacc ggcagagctg     4320

tttgcgcgtc tgaaagcgag catgccggat ggcgaagatc tggtggtgac ccatggtgat     4380

gcgtgcctgc cgaacattat ggtggaaaat ggccgtttta gcggctttat tgattgcggc     4440

cgtctgggcg tggcggatcg ttatcaggat attgcgctgg ccacccgtga tattgcggaa     4500

gaactgggcg gcgaatgggc ggatcgtttt ctggtgctgt atggcattgc ggcaccggat     4560

agccagcgta ttgcgtttta tcgtctgctg gatgaatttt tctaataact gtcagaccaa     4620

gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag     4680

gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac     4740

tgagcgcggc cgc                                                        4753


<210> 3
<211> 4515
<212> DNA
<213> plasmids


<220> 
<223> pUCZM-7 (P3)

<400> 3
gtcgacggta ccatgattat cggcttctca aaacatggac agggcaaagc tgctccagcc       60

ataaaatacc tgctcgatca gcacagagag ggcagagaaa atcatattcc tgaagtggtg      120

agaggtaatc cagacttaac cgcatccatt atcgacagcc tgactttcaa acatcactat      180

actagcggag ttctttcttt cgctgccgaa gatcatgttt ctgccgagca ggaaaaagat      240

attatcaacg atttcgaaaa gctggctttt gctggcctgc aagcagacca gtttaatatc      300

ctttgggtgc gccattctca tgcgggtcat catgaacttc atttcatcac tccaaggata      360

gaactttcat ccaagaagtc cttcaacatc aagccccccg gtaagaatac ccaaaaggaa      420

tttgatgact tgcggagtgc catcaatgcc aaatatggct ttgctgaccc agaagacccg      480

atgagagcga gaaacgtcaa aacacctagc tatgaactaa aaatcgctag gaatgtcctt      540

agagagcaaa aagcgccctc tgaggacata agacagctta ttgatagcct tttaactgaa      600

agagccgtcc aaggcctgat acatgacaga aaaactcttc tcactaatgt tgaagagtta      660

ggcttcgaag ttactcgcgc aggcaagaat tatattaccg tcaaagaccc tgaaagctca      720

aaacgatttc gtctcaaagg ccaactctat gaacaagact ttacctctag ccgaacgatt      780

gaaacagcag cagctaaaag aacaagagat tattctcgac ctgactcaac agcagctaga      840

caatttgagg aacaagttaa actccacatt cgaaaacgag ctgaattcaa tagaaaacga      900

tatcgagcga ctgaaccgga gcttaggatg gaaattcctc ctatctcgct ggctgatacc      960

agtcgtgacc agcatcatga tattgtttgg cctgctcttg ggcgcagaag ggataatcaa     1020

gctctatacc caccagatca gagctttgca ggaggaaaaa gcagagatgg aagtgaccgt     1080

cgaacagctc aacaaagcag gtgggaagat gcaattcagc cattgcggag aaaaagaaag     1140

gctttgcatc agagtaaaca cacgagagat cttcgggata accagatatt atctgatcgt     1200

gaaagaacgg aaatagacaa tgacggaatt ggaacaggtt ttactgaacg ccttggaaaa     1260

tttacagcag agattcgatc aacaacagaa aagtttatta cacgcgcaaa gcgaattacg     1320

gcagatgttc gaaactacct caaccgagaa tacttattta cgacagcaag tagaaatctt     1380

ggtcgagcga ttgaacagtt ttcccagatg aaaaccttaa gtcgaaagct acaaaaacgg     1440

agaggtcgtt ctcgctctcg ataaacttga aaaggacgct aattccgaaa agttttttat     1500

ctttctgata gtcgatttta gttggcgatt tccgataaaa aagccctgaa cttattgaac     1560

tttcctatat ttcaataagt tcaataagtt cagggctttt ttattcaaaa aaatcaatgc     1620

cttacgactt taaaaaccac ttttggggag tgaaaaacca cttttgggga gtgaaaaacc     1680

acttttgggg agtgaaaaac cacttcaaaa aatatattaa gtggttaata aagttgtaat     1740

gagaaaaaca ctacaaataa ttaaagatag agccggcaat aaagatcaaa atttacatgc     1800

aggtgaaatt atagatggac atttttcaga ccttaaaaat ccgcctagcc tagctgcact     1860

aaaactctat gagttgctta ttaaatacgc agcatataat atagataaaa atatatggca     1920

taatatttct gtatcagaaa tgcaaaaaat caaaggaata aagaactata actcaacaga     1980

gctaactcaa ttattaaaag aacttagagc tgttgttatg gaatatagag caaaagatag     2040

aactattatt actggtcttt tagatattgc tgcggtcata tctaacccag acacaggata     2100

tccagagata caatggaaat ttggagaaag ttttagagat ttaatagcta agtcagaata     2160

ttgggcaatt gttgattata gaacaacgtt agagatgacg agccgatatt cccttcgttt     2220

atatgaaata ttagctctta gaataaattt aaaacacaaa aattcagata catttacttt     2280

agccgagctt agaggaaaac tgggagttcc tgaaggaaaa ttaaaaagat ggtctgattt     2340

aagagataaa gcgatcaatg ttgcggtaaa agaaattaac caacggacta ctttcgccgt     2400

aacagcacaa ccaaaaaaaa tgggaagatc cgttcaagcc gtagaattta aatgggaacg     2460

aaaagagatt aaacttcctc aaagtatacc attaaaaaca tctaaaagca ttgtttttcc     2520

attagatgga agtattagat atactcaatg ggaagatatt gcacgaaaaa atcttcctaa     2580

gcctttacct gatttagata tggtagcaca acaatttaga aaatggttaa aaaataaaaa     2640

catttcatca aacagtatta atgttttgtc catttttaag aaattttgtg aaaaatacag     2700

aatagaattt aataattaag aattcagtac tcccggggga tcctctagac tgcagaagct     2760

tataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt     2820

gaacgctctc ctgagtagga caaatccgcc gggagcggat ttgaacgttg cgaagcaacg     2880

gcccggaggg tggcgggcag gacgcccgcc ataaactgcc aggcatcaaa ttaagcagaa     2940

ggccatcctg acggatggcc tttgtagaaa agatcaaagg atcttcttga gatccttttt     3000

ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt     3060

tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga     3120

taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag     3180

caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata     3240

agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg     3300

gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga     3360

gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca     3420

ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa     3480

acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt     3540

tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac     3600

ggttcctggc cttttgctgg ccttttgctc acatatgttc aaatatgtat ccgctcatga     3660

gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg gaaaaaaaaa     3720

tcaccggcta taccaccgtg gatattagcc agtggcatcg taaagaacat tttgaagcgt     3780

ttcagagcgt ggcgcagtgc acctataacc agaccgtgca gctggatatc accgcgtttc     3840

tgaaaaccgt gaaaaaaaac aaacacaaat tctacccggc gtttattcat attctggccc     3900

gtctgatgaa cgcgcatccg gaatttcgta tggccatgaa agatggcgaa ctggtgattt     3960

gggatagcgt gcatccgtgc tataccgtgt ttcatgaaca gaccgaaacc tttagcagcc     4020

tgtggagcga atatcatgat gattttcgcc agttcctgca tatttatagc caggatgtgg     4080

cgtgctatgg cgaaaacctg gcctattttc cgaaaggctt catcgaaaac atgttctttg     4140

tgagcgcgaa tccgtgggtg agctttacca gcttcgatct gaacgtggcg aacatggata     4200

acttttttgc gccggtgttt accatgggca aatattatac ccagggcgat aaagtgctga     4260

tgccgctggc cattcaggtg catcatgcgg tgtgtgatgg ctttcatgtg ggccgtatgc     4320

tgaacgaact gcagcagtat tgtgatgaat ggcagggcgg tgcgtaataa ctgtcagacc     4380

aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct     4440

aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc     4500

actgagcgcg gccgc                                                      4515


<210> 4
<211> 8603
<212> DNA
<213> cassette


<220> 
<223> Gene cassette (G1)

<400> 4
tctagattca aggtgtcccg ttcctttttc ccctttttgg aggttggtta tgtcctataa       60

tcacttaatc cagaaacggg cgtttagctt tgtccatcat ggttgtttat cgctcatgat      120

cgcggcatgt tctgatattt ttcctctaaa aaagataaaa agtcttttcg cttcggcaga      180

agaggttcat catgaacaaa aattcggcat ttttaaaaat gcctatagct aaatccggaa      240

cgacacttta gaggtttctg ggtcatcctg attcagacat agtgttttga atatatggag      300

taagcaatgg cgctggtttc tatgcgtcag ctgctggacc acgcggcgga aaactcttac      360

ggtctgcctg cgttcaacgt taacaacctg gaacagatgc gtgcgatcat ggaagcggcg      420

gaccaggtta acgcgccggt tatcgttcag gcgtctgcgg gtgcgcgtaa atacgcgggt      480

gcgccgttcc tgcgtcacct gatcctggcg gcggttgaag aatttccgca catcccggtt      540

gttatgcacc aggaccacgg tgcgtctccg gacgtttgcc agcgttctat ccagctgggt      600

ttctcttctg ttatgatgga cggttctctg ctggaagacg gtaaaacccc gtctacctac      660

gaatacaacg ttaacgcgac ccgtaccgtt gttaacttct ctcacgcgtg cggtgtttct      720

gttgaaggtg aaatcggtgt tctgggcaac ctggaaaccg gtgaagcggg tgaagaagac      780

ggtgttggtg cggcgggtaa actgtctcac gaccagatgc tgacctctgt tgaagacgcg      840

gttcgtttcg ttaaagacac cggtgttgac gcgctggcga ttgcggttgg cacctctcac      900

ggtgcgtaca aattcacccg tccgccgacc ggtgacgttc tgcgtatcga ccgtatcaaa      960

gaaatccacc aggcgctgcc gaacacccac atcgttatgc acggttcttc ttctgttccg     1020

caggaatggc tgaaagttat caacgaatac ggtggtaaaa tcggtgaaac ctacggtgtt     1080

ccggttgaag aaatcgttga aggtatcaaa cacggtgttc gtaaagttaa catcgacacc     1140

gacctgcgtc tggcgtctac cggtgcgatc cgtcgtttca tggcggaaaa cccgtctgaa     1200

tttgacccgc gtaaatacct gtctaaaacc gttgaagcga tgaaacagat ttgcctggac     1260

cgttacctgg cgttcggttg cgaaggtcag gcgggtaaaa tcaaaccggt ttctctggaa     1320

aaaatggcga accgttacgc gaaaggtgaa ctgaaccaga tcatcaaata atttaccaac     1380

actactacgt ataaggagag cgttgttatg cgtcgtaaac tggttgcggg taactggaaa     1440

atgaacggtt ctcgtgcgat gctggttgaa ctggacgcga tcgcggcggc ggcgtctgcg     1500

gctccggaaa tcgacgtttc tgttgcggtt ccgttcaccc tgatcgcggc ggcgtctgaa     1560

cgtgttccga ccctggcgat cggtgcggaa gacgttcacg aagcggaatc tggtgcgcac     1620

accggttgcg tttctgcgtc tatggttaaa gaagttggtg cgtctttcac catcgttggt     1680

cactctgaac gtcgttctga ccagcacgaa acctctcacg acgcgtgggc gaaagcggcg     1740

gcggcgcaca aacagggtct gaacgttatc ctgtgctgcg gtgaaaccga agcggaacgt     1800

gacgcggacc gtgcggaacg tgttgttcag gcgcagatcg aaaaatctgt tccggacaac     1860

gcggacggtt cttggttcac cctggcgtac gaaccgcgtt gggcgatcgg caccggtcgt     1920

accccgaccc tggaagaaat cgcggcgatc cacgcgatcg cgcgtgcgaa actgcgtatg     1980

ctgatcggtg acaaagcggc gggtgttcgt atcctgtacg gtggttctgt taccggtgcg     2040

aacgcggcgt ctatcctgga caccccgaac gttgacggtg cgctggttgg tggtgcgtct     2100

ctgaccgcgg cgaaattcgt tccgatcatc gaagcggcgg cgtctctgta atttaccaac     2160

actactacgt ataaggagag cgttgttatg gaaatcgttg cgatcgacat cggtggcacc     2220

cacgcgcgtt tctctatcgc ggaagtttct aacggtcgtg ttctgtctct gggtgaagaa     2280

accaccttca aaaccgcgga acacgcgtct cttcagctgg cgtgggaacg tttcggtgaa     2340

aaactgggtc gtccgctgcc gcgtgcggcg gcgatcgcgt gggcgggtcc ggttcacggt     2400

gaagttctga aactgaccaa caacccgtgg gttctgcgtc cggcgaccct gaacgaaaaa     2460

ctggacatcg acacccacgt tctgatcaac gacttcggtg cggttgcgca cgcggttgcg     2520

cacatggact cttcttacct ggaccacatc tgcggtccgg acgaagcgct gccgtctgac     2580

ggtgttatca ccatcctggg tccgggcacc ggtctgggtg ttgcgcacct gctgcgtacc     2640

gaaggtcgtt acttcgttat cgaaaccgaa ggtggtcaca tcgacttcgc gccgctggac     2700

cgtctggaag acaaaatcct ggcgcgtctg cgtgaacgtt tccgtcgtgt ttctatcgaa     2760

cgtatcatct ctggtccggg tctgggtaac atctacgaag cgctggcggc gatcgaaggt     2820

gttccgttct ctctgctgga cgacatcaaa ctgtggcaga tggcgctgga aggtaaagac     2880

aacctggcgg aagcggcgct ggaccgtttc tgcctgtctc tgggtgcgat cgcgggtgac     2940

ctggcgctgg cgcagggtgc gacctctgtt gttatcggtg gtggtgttgg tctgcgtatc     3000

gcgtctcacc tgccggaatc tggtttccgt cagcgtttcg tttctaaagg tcgtttcgaa     3060

cgtgttatgt ctaaaatccc ggttaaactg atcacctacc cgcagccggg tctgctgggt     3120

gcggcggcgg cgtacgcgaa caaatactct gaagttgaat aatttaccaa cactactacg     3180

tataaggaga gcgttgttat ggcggacctg ccggcgaccc cgctgctgga caccgtttct     3240

accccggacg acctgcgtaa actgcgtccg gaccagctgc gtcagctggc ggacgaactg     3300

cgtaccgaaa ccatctctgc ggttggcacc accggtggtc acctgggttc tggtctgggt     3360

gttgttgaac tgaccaccgc gatccactac gttttcgaca ccccgcgtga ccgtctggtt     3420

tgggacgttg gtcaccagtg ctacccgcac aaaatcctga ccggtcgtcg tgaccgtatc     3480

cgtaccctgc gtcagggtgg tggtctgtct ggtttcacca aacgttctga atctgaatac     3540

gacccgttcg gtgcggcgca ctcttctacc tctatctctg cggcgctggg tttcgcggtt     3600

gcgaacaaaa tcgcgggcac ccctggtaaa ggtatcgcgg ttatcggtga cggtgcgatg     3660

tctgcgggta tggcgtacga agcgatgaac aacgcggcgc aggcgggtaa ccgtctggtt     3720

gttatcctga acgacaacga catgtctatc gcgccgccgg ttggtggtct gtctgcgtac     3780

ctgtctcgta tcgtttcttc tcgtgaattt ctgggtttcc gtgacctggc gcgtaaaatc     3840

gcgaaacgtc tgccgcgtcc gatcgcgtct ggtatgcgta aaaccgacga atttgcgcgt     3900

ggtatgacca tgggtggcac cctgttcgaa gaactgggtt tctactacgt tggtccgatc     3960

gacggtcaca acctggacca cctgatcccg gttctggaaa acgttcgtga cgcggaagaa     4020

ggtccgatcc tggttcacgt tgttaccaaa aaaggtaaag gttacgcgcc ggcggaagcg     4080

gcggcggaca aataccacgg tgttcagaaa ttcgacgtta tctctggtgt tcagaccaaa     4140

gcgccgccgg gtccgccgca gtaccagaac gttttcggtg cgcagctggt tgcggaagcg     4200

gcgaaagacg cgcgtatctg cgcgatcacc gcggcgatgc cgtctggcac cggtctggac     4260

gcgttcgcga aagcgtaccc ggaccgtttc ttcgacgttg gtatcgcgga acagcacggt     4320

gttaccttcg cggcgggtct ggcggcgcag ggtctgcgtc cgttctgcgc gatctactct     4380

accttccttc agcgtgcgta cgaccaggtt gttcacgacg ttgcgatcca gaacctgccg     4440

gttcgtttcg cgatcgaccg tgcgggtctg gttggtgcgg acggtgcgac ccacgcgggt     4500

tctttcgacg ttacctacct ggcgaccctg ccgaacttcg ttgttatggc ggcggcggac     4560

gaagcggaac tggttcacat gacccacacc gcggttcagt acgacgacgg tccgatcgcg     4620

ctgcgttacc cgcgtggtaa cggcaccggt gttgcgctgc cggaaacccc gcagctgctg     4680

gaaatcggta aaggtcgtgt tgttcgtgaa ggtaaaaccg ttgcgatcct gtctctgggc     4740

acccgtctgg aagaagcgct gaaagcggcg gcgatcctgg aagcgaaagg tctgtctacc     4800

accgttgcgg acctgcgttt cgcgaaaccg ctggacgttg acctgatccg taaactgctg     4860

accacccacg aagttgcggt taccatcgaa gaaaactctg ttggtggttt cggtgcgcac     4920

gttctgacca tggcgtctga cgaaggtctg atcgacggtg gtctgaaact gcgtaccctg     4980

cgtctgccgg acatgtacca ggaccaggac aaaccggaag ttcagtacgc ggaagcgggt     5040

ctggacgcga ccgcgctggt tgacaccgtt ctgaaagcgc tgcgtcacaa ctctggtggt     5100

gttgcggaag cgcgtgcgta atttaccaac actactacgt ataaggagag cgttgttatg     5160

gtttcttacc tggacttcga aaaaccggtt gcggaacttc agacccgtat cgcggaactg     5220

cgtaaagcga cctctgacga aatggacctg gaccgtgaaa tctctgacct ggaagttaaa     5280

gttcagaaac tgctgcgtga cacctacgcg cacctgaccc cgtggcagaa aacccaggtt     5340

gcgcgtcacc cggaacgtcc gcacttccac gactacatct ctgcgctgtg cgaagaattt     5400

atcccgctgg cgggtgaccg tctgttcggt gaagacgaag cgatcatcgg tggtctggcg     5460

cgtatcgacg gtcagcgtgt tatgatcatc ggtcaggaaa aaggtaacga caccgcgtct     5520

cgtctgaaac acaacttcgg tatggcgcgt ccggaaggtt accgtaaagc gatccgtctg     5580

atgaaactgg cggaccgttt cggtctgccg gttatcaccc tggttgacac ctctggtgcg     5640

ttccctggta tcgacgcgga agaacgtggt caggcggaag cgatcgcgcg ttctaccgaa     5700

acctgcctgt ctctgggtgt tccgctgatc tctgttatcg ttggtgaagg tggttctggt     5760

ggtgcgatcg cgatcgcggc ggcgaaccgt ctgctgatgt tcgaacacgc ggtttactct     5820

gttatctctc cggaaggttg cgcgtctatc ctgtggcgtg acgcgggtaa agcgtctgac     5880

gcggcgaccg cgatgaaact gaccgcgacc gacctgtacg gtctgggtat cgttgaccgt     5940

atcgttctgg aaccggttgg tggtgcgcac cgtgacccga aaaccgcgat cgacgcgctg     6000

aaaatggcgc tgcttcagga actggcgttc ctgaaaccga tggaccgtga cgcgctgcgt     6060

tctatgcgtc gtaaaaaatt cctggacatc ggtggttaat ttaccaacac tactacgtat     6120

aaggagagcg ttgttatgtc ttggctggaa aaagttcgta acaaaatccc gttcatcgtt     6180

aaacgtgaaa ccccggacaa cctgtggcac cgttgcccgc actgccagac catgatcttc     6240

aacaaagact acgaagaaaa cctgtctgtt tgcccgaaat gcggtttcca cggtcgtatc     6300

ggttctcgtg cgcgtttcga ctctctgttc gacgaaggtg actactctgt tctgccgccg     6360

ccggttgttc cggaagaccc gctgaaattc cgtgacacca aacgttacgt tgaccgtctg     6420

aaagcggcgc gtctgaaatc tggtgaaaac gacgcgctgc tgaacgcgaa aggtcgtctg     6480

gcgggtcgtc agaccgttat cggtgttcag gacttctctt tcatgggtgg ttctatgggt     6540

ctggcggttg gttctgcgtt cgttgcgggt gttaaagcgg cgatcgaatg ccactctccg     6600

tacatcatct tcaccgcggc gggtggtgcg cgtatgcagg aaggtatcct gtctctgatg     6660

cagatgccgc gttctaccgc ggcgatcgcg gaactgcgtg aagcgggtct gccgtacatc     6720

gttgttctga ccgacccgac caccggtggt gttaccgcgt cttacgcgat gctgggtgac     6780

gttcagatcg cggaaccggg tgcgctgatc ggtttcgcgg gtcagcgtgt tatcgaatct     6840

accatccgtg aaaaactgcc ggaaggtttc cagcgtgcgg aatacctgct ggaacacggt     6900

atgatcgaca tggttgttcc gcgtgcggaa atgcgtgacc gtctgatctc tctggttgac     6960

tacctgctgc cggcgaacga agcggcgtaa tttaccaaca ctactacgta taaggagagc     7020

gttgttatga accacctgcg tgcggaaggt ccggcgtctg ttctggcgat cggcaccgcg     7080

aacccggaaa acatcctgct tcaggacgaa tttccggact actacttccg tgttaccaaa     7140

tctgaacaca tgacccagct gaaagaaaaa ttccgtaaaa tctgcgacaa atctatgatc     7200

cgtaaacgta actgcttcct gaacgaagaa cacctgaaac agaacccgcg tctggttgaa     7260

cacgaaatgc agaccctgga cgcgcgtcag gacatgctgg ttgttgaagt tccgaaactg     7320

ggtaaagacg cgtgcgcgaa agcgatcaaa gaatggggtc agccgaaatc taaaatcacc     7380

cacctgatct tcacctctgc gtctaccacc gacatgccgg gtgcggacta ccactgcgcg     7440

aaactgctgg gtctgtctcc gtctgttaaa cgtgttatga tgtaccagct gggttgctac     7500

ggtggtggca ccgttctgcg tatcgcgaaa gacatcgcgg aaaacaacaa aggtgcgcgt     7560

gttctggcgg tttgctgcga catcatggcg tgcctgttcc gtggtccgtc tgaatctgac     7620

ctggaactgc tggttggtca ggcgatcttc ggtgacggtg cggcggcggt tatcgttggt     7680

gcggaaccgg acgaatctgt tggtgaacgt ccgatcttcg aactggtttc taccggtcag     7740

accatcctgc cgaactctga aggcaccatc ggtggtcaca tccgtgaagc gggtctgatc     7800

ttcgacctgc acaaagacgt tccgatgctg atctctaaca acatcgaaaa atgcctgatc     7860

gaagcgttca ccccgatcgg tatctctgac tggaactcta tcttctggat cacccaccct     7920

ggtggtaaag cgatcctgga caaagttgaa gaaaaactgc acctgaaatc tgacaaattc     7980

gttgactctc gtcacgttct gtctgaacac ggtaacatgt cttcttctac cgttctgttc     8040

gttatggacg aactgcgtaa acgttctctg gaagaaggta aatctaccac cggtgacggt     8100

ttcgaatggg gtgttctgtt cggtttcggt ccgggtctga ccgttgaacg tgttgttgtt     8160

cgttctgttc cgatcaaata ctaatttacc aacactacta cgtataagga gagcgttgtt     8220

atggcggtta aacacctgat cgttctgaaa ttcaaagacg aaatcaccga agcgcagaaa     8280

gaagaatttt tcaaaaccta cgttaacctg gttaacatca tcccggcgat gaaagacgtt     8340

tactggggta aagacgttac ccagaaaaac aaagaagaag gttacaccca catcgttgaa     8400

gttaccttcg aatctgttga aaccatccag gactacatca tccacccggc gcacgttggt     8460

ttcggtgacg tttaccgttc tttctgggaa aaactgctga tcttcgacta caccccgcgt     8520

aaataatatt ttttaatatt atgaactgaa tttaagaggc tgcctcccga ttttatcgga     8580

aggcagcctt ttttatactg cag                                             8603


<210> 5
<211> 8033
<212> DNA
<213> cassette


<220> 
<223> Gene cassette (G2)

<400> 5
tctagaatca acaacccgaa tcctatcgta atgatgtttt gcccgatcag cctcaatcga       60

caattttacg cgtttcgatc gaagcaggga cgacaattgg ctgggaacgg tatactggaa      120

taaatggtct tcgttatggt attgatgttt ttggtgcatc ggccccggcg aatgatctat      180

atgctcattt cggcttgacc gcagtcggca tcacgaacaa ggtgttggcc gcgatcgccg      240

gtaagtcggc acgttaaaaa atagctatgg aatatagtag ctacttaata agttaggaga      300

ataaacatga aatctgttac catcctgggt gcgaccggtt ctgttggttc ttctaccctg      360

gacctgatcg aacgtgaacc ggaccgtttc cgtgttgttg cgctgaccgc gaactgcgac      420

gttgcgaaac tggcggcggc ggcgatccgt accaaagcgg aatttgcggt tgttgcggac      480

gcgggttgcc tgccggacct gcgtgacgcg ctggcgggca ccggtatccg tgcgggtggt      540

ggtgcggaag cgatcgcgga agcggcggcg tctggtgcgg actggaccat gggtgcgatc      600

gttggttgcg cgggtctgaa accggttatg gcggcgatcg aacagggtgg caccgttgtt      660

atcgcgaaca aagaaccgct ggtttctgcg ggtgacgtta tcctggcggc ggcgaaacgt      720

accggtgcga ccctgctgcc tgcggactct gaacacaacg cgatcttcca gtgcttcgac      780

gcgtctcgtc cggaacgtgt tcgtcgtatc atcctgacct gctctggtgg tccgttccgt      840

gactggacca ccgaacagat gcgtggtgtt accctggaac aggcggttaa acacccgaac      900

tggtctatgg gtgcgaaaat ctctgttgac tctgcgacct gcatgaacaa aggtctggaa      960

ctgatcgaag cggcgcgtct gttcccgatc ccgcacgacc gtatcgaaat cgttatccac     1020

ccgcagtctg ttgttcactc tctggttgac tacgttgacg gttctgttct ggcgcagctg     1080

ggtccgccgg acatgcgtac cccgatcgcg cacaccctgg cgtggccgga ccgtatggcg     1140

accccgatgg cgccgctgga cctggttgcg atcggtcgtc tggacttcga agcgccggac     1200

ccggttcgtt tcccggcgct gcgtctggcg cgtgaagcgc tggacgcggg tggtgcgcgt     1260

ccggcgatcc tgaacgcggc gaacgaagtt gcggttgaag cgttcctgaa acgtcgtatc     1320

cgtttcctgg aaatcgcggc gatcgttgaa gacaccctgg aacgttacaa cccggcggcg     1380

ccggactctg ttgacgcggt tctggcgatc gacgcggaag cgcgtgttct ggcgggtgaa     1440

cgtgttaaag actgcgcggt ttaatttacc aacactacta cgtataagga gagcgttgtt     1500

atggcgcacg gtcagcgtat cgttgcgatc atcgttgcgg cgggcaccgg tgttcgtgcg     1560

ggtggtgcgg ttccgaaaca gtacgcgcgt atcggtggtc gtccgatgat cgcgtactct     1620

tacgacgcgt tcgcggcgca cccggcgatt gttgaaacca tcgttgttgt tgcggacggt     1680

cagcaggaca tggcggttga cgcgctgggt ggtgacgcga ccatcgttgt tggtggtgcg     1740

acccgtcgtg aatctgttgc gaacggtgtt gcggcggttg gtgcggcgac ccacgttctg     1800

atccacgacg cggcgcgtcc gttcctgtct gcggcggtta tcgactctct ggttgttgcg     1860

ctgaaaaccc gtgacgcggc gatcccggtt ctgccggtta ccgacaccct ggcgcgtggt     1920

tctgttctgc tgggtgacat cgttccgcgt gcggacctga accgtgttca gaccccgcag     1980

gcgttcgcgg ttgaaggtct gcgtgcggcg cacgcggttt ggccggcgga cgaagaagcg     2040

accgacgacg cgcagatggt tcgtcgtctg ggtgttggtg ttgcgctggt tcagggttct     2100

gcgatgctgg aaaaagttac ccacccggaa gacttcgcgg cggcggaagc gcgtgttgcg     2160

gcgaaaacct cttacgaaac ccgtaccgcg atgggtttcg acgttcaccg tctggaaacc     2220

ggtgaagaac tgtggctggg tggtgttctg atcccgcacg acaaaggtct gtctggtcac     2280

tctgacgcgg acgttgcgct gcacgcgctg accgacgcgc tgctgggcac catcgcggcg     2340

ggtgacatcg gcacccactt cccgccgtct gacccgcagt ggaaaggtgc ggactctgcg     2400

cagttccttc agcacgcggc gaaactgatc gcggacaaac acggtcgtat cgacttcgtt     2460

gacctgacca tcatgtgcga agcgccgaaa atcggtccgc accgtgcggc gatgaccgcg     2520

cgtatcgcgg acctgctggg tctgaccctg gaccgtgttt ctgttaaagc gaccaccacc     2580

gaacgtctgg gtttcaccgg tcgtggtgaa ggtatcgcgg cgcaggcggt tgcgaccgtt     2640

cgtgttccgg gtgcgccggc gtaatttacc aacactacta cgtataagga gagcgttgtt     2700

atgccggcga ccaccgaacc ggcgccggcg aaaatcaacc tggcgctgca cgttcgtcgt     2760

cgtcgtgacg acggttacca cgaactggaa accctgttcg cgttcgcgac cgacggtgac     2820

atcgttaccg ttgaaccggc ggcggcgaac tctttcacca tcaccggtcc gttcgcggac     2880

gcgctgctgc cggcgccgaa acacaacacc accccggcgt ctgcgggtgt tgcggcgtgg     2940

ggtggtaacc tggttaccgc ggcggcggac gcgttcaccg cggcgttcgc gccggacacc     3000

caccacgcga tcaccctgga aaaacacctg ccgatcgcgt ctggtatcgg tggtggttct     3060

gcggacgcgg cggcgaccct gcgtgcgctg gcgcgtctgc actgcgttgg tccgaccgac     3120

ccgcgtctgc acgcgatcgc ggcgaccctg ggtgcggacg ttccggcgtg cctggcgaac     3180

cgtaccaccc tgggcaccgg taaaggtgac accctggcga acctggaagg tctgtctggc     3240

accccgctgc tgctggttaa ccctggtgtt gcggtttcta ccgcggcggt tttcaaaacc     3300

tgggacggtc aggaccgtgg tccgatcccg gaaggtgacc tgctgacccg tgcgaccgcg     3360

ggtcgtaacg acctggaacc gccggcgctg gcgctggcgc cggttatcgc ggacgttcgt     3420

accctgctgg acgcggcgca cgaagttctg ctgtctcgta tgtctggttc tggtgcgacc     3480

tgcttcgcgc tgttcgaaac cgcggaagcg cgtgacgcgg cggcgaccgc ggcgctggcg     3540

cgtggttggt ggaccctggc gaccaccctg gcgtaattta ccaacactac tacgtataag     3600

gagagcgttg ttatgtctct gcgtccgtgg cgtgacatca cccgtcgtac ctctcgtcag     3660

atcatggttg gcaccgttcc ggttggtggt gacgcgccgg ttaccgttca gaccatgacc     3720

aacaccccga ccgacgacgt taaagcgacc gttgaccaga tccgtcgttg cgaagaagcg     3780

ggtgttgaca tcatccgtgt ttcttgcccg gacgttgaat ctaccaccgc gctgaaacag     3840

atcgttcgtg cgtctcgtgt tccgatcgtt gcggacatcc acttccacta caaacgtgcg     3900

ctggaagcgg cggacgcggg tgcggcgtgc ctgcgtatca accctggtaa catcggttct     3960

gcggcgcgtg ttaaagaagt tgttgacgcg gcgaaatcta acggttgcgc gatccgtatc     4020

ggtgttaacg gtggttctct ggaacgtcac ctgctggaaa aatacggtga accgtgcccg     4080

gacgcgctgg ttgaatctgc gatggaccac atcaaactgc ttcaggacca cgacttccac     4140

gaatttaaag ttgcggttaa agcgtctgac ctgttcctgg cggttgcggc gtaccagcag     4200

ctggcggaac aggttgactg cccgctgcac ctgggtatca ccgaagcggg tggtttcgtt     4260

ggtggcaccg ttaaatctgc gatcggtatg ggttctctgc tgtggtacgg tatcggtgac     4320

accatccgtg tttctctgtc tgcggaaccg gaagaagaag ttcgtgttgg tttcgaaatc     4380

ctgaaagcgc tgggtatccg taaccgtggt gttcgtgttg tttcttgccc gtcttgcgcg     4440

cgtcagggtt tcgacgttat ccgtaccgtt caggcgctgg aagaacgtct tcagcacatc     4500

cgtaccccga tgtctctgtc tgttctgggt tgcgttgtta acggtccggg tgaagcgcgt     4560

gaaaccgaca tcggtatcac cggtggtggt aacggtaaac acatggttta cctgtctggt     4620

gttaccgacc accacgttca ggacgcggac atggttgacc acatcgttcg tctggttgaa     4680

gcgaaagcgg cggaaatcga agcggcggac gcggcgatcc tggcgtctgt tgcggcgtaa     4740

tttaccaaca ctactacgta taaggagagc gttgttatgc agatcctgct ggcgaacccg     4800

cgtggtttct gcgcgggtgt tgaccgtgcg atctctatcg ttgaaaacgc gctggcgatc     4860

tacggtgcgc cgatctacgt tcgtcacgaa gttgttcaca accgttacgt tgttgactct     4920

ctgcgtgaac gtggtgcgat cttcatcgaa cagatctctg aagttccgga cggtgcgatc     4980

ctgatcttct ctgcgcacgg tgtttctcag gcggttcgta acgaagcgaa atctcgtgac     5040

ctgaccgttt tcgacgcgac ctgcccgctg gttaccaaag ttcacatgga agttgcgcgt     5100

gcgtctcgtc gtggtgaaga atctatcctg atcggtcacg cgggtcaccc ggaagttgaa     5160

ggcaccatgg gtcagtactc taacccggaa ggtggtatgt acctggttga atctccggac     5220

gacgtttgga aactgaccgt taaaaacgaa gaaaaactgt ctttcatgac ccagaccacc     5280

ctgtctgttg acgacacctc tgacgttatc gacgcgctgc gtaaacgttt cccgaaaatc     5340

gttggtccgc gtaaagacga catctgctac gcgaccacca accgtcagga agcggttcgt     5400

gcgctggcgg aacaggcgga agttgttctg gttgttggtt ctaaaaactc ttctaactct     5460

aaccgtctgg cggaactggc gcagcgtatg ggtaaacgtg cgttcctgat cgacgacgcg     5520

aaagacatcc aggaagaatg ggttaaaggt gttaactgcg ttggtgttac cgcgggtgcg     5580

tctgcgccgg acatcctggt tcagaacgtt gttgcgcgtc tgcaccagct gggtggtggt     5640

gaagcgatcc cgctggaagg tcgtgaagaa aacatcgttt tcgaagttcc gaaagaactg     5700

cgtgttgaca tccgtgaagt tgactaattt accaacacta ctacgtataa ggagagcgtt     5760

gttatgttct acctgggtga atttggtctg gaccgtaaag accagcacgt tggtctggcg     5820

aaccagcagt actctgcgac cccggcgaaa gacttcaccg aaaccctgtt cgttcaccac     5880

tctctgccgc agaccaaagt tgacgaagtt gacatctcta cctctgttgc gggtctggac     5940

ttcgcgttcc cgttcttcat caacgcgatg accggtggtt ctaaaaaaac ccgtgaaatc     6000

aaccgtctgc tgggtatcat gggtcacttc ggtaaaatcg cgctggcgtc tggttctgtt     6060

tctgcggcga tcaaagaccc gtctgttgcg gaaaccttct ctgttatgcg tcgtgaaaac     6120

ccgtacggta tcatcttcgc gaacctgggt gcgcaccact ctgttgaaaa cgcgaaacgt     6180

gcggttgacc tgctggaagc gaacgcgatc cagatccacg ttaacgcgcc gcaggaaatc     6240

gttatgccgg aaggtgaccg tgacttcacc atgtggctga aaaacatcga aaccctggtt     6300

cgtgaaatgg aagttccggt tatcgttaaa gaagttggtt tcggtatgtc tcgtgaaacc     6360

gttgcgcagc tggcgtctgc gggtgttcag accatcgacg tttctggcac cggtggcacc     6420

gacttcgcga aaatcgaaaa cgcgcgtcgt accttcaacg actacgcgta cctggaaggt     6480

tggggtcagt ctaccgttac ctctctggtt gaagcgatgt ctgtttctga agaagtttgc     6540

ccgtctctga tcgcgtctgg tggtatcaaa accccgctgg acatcgttaa atctctggcg     6600

ctgggtgcgg acctggttgg tatgtctaac cacttccttc agtacgttaa agacggtaaa     6660

ggtcaccgtt tcgacgacgg tcttcaggcg atcaaagttt accagtggca gatcgcggaa     6720

atcatgacca tgctgggtgc gaaaaacatc gcggaactgc gtcagaaaga cctggttctg     6780

gcgccgaacg ttcagaactg gtgcgaagcg cgtggtatcg actggaaagc gtacgcgcgt     6840

cgttctgcga acgtttctta atttaccaac actactacgt ataaggagag cgttgttatg     6900

gcgggtgcga tcaccgacca gctgcgtcgt tacctgcacg gtcgtcgtcg tgcggcggcg     6960

cacatgggtt ctgactacga cggtctgatc gcggacctgg aagacttcgt tctgggtggt     7020

ggtaaacgtc tgcgtccgct gttcgcgtac tggggttggc acgcggttgc gtctcgtgaa     7080

ccggacccgg acgttctgct gctgttctct gcgctggaac tgctgcacgc gtgggcgctg     7140

gttcacgacg acctgatcga ccgttctgcg acccgtcgtg gtcgtccgac cgcgcagctg     7200

cgttacgcgg cgctgcaccg tgaccgtgac tggcgtggtt ctccggacca gttcggtatg     7260

tctgcggcga tcctgctggg tgacctggcg caggtttggg cggacgacat cgtttctaaa     7320

gtttgccagt ctgcgctggc gccggacgcg cagcgtcgtg ttcaccgtgt ttgggcggac     7380

atccgtaacg aagttctggg tggtcagtac ctggacatcg ttgcggaagc gtctgcggcg     7440

gaatctatcg aatctgcgat gaacgttgcg accctgaaaa ccgcgtgcta caccgtttct     7500

cgtccgcttc agctgggcac cgcggcggcg gcggaccgtt ctgacgttgc ggcgatcttc     7560

gaacacttcg gtgcggacct gggtgttgcg ttccagctgc gtgacgacgt tctgggtgtt     7620

ttcggtgacc cggcggttac cggtaaaccg tctggtgacg acctgaaatc tggtaaacgt     7680

accgttctgg ttgcggaagc ggttgaactg gcggaccgtt ctgacccgct ggcggcgaaa     7740

ctgctgcgta cctctatcgg cacccgtctg accgacgcgc aggttcgtga actgcgtacc     7800

gttatcgaag cggttggtgc gcgtgcggcg gcggaatctc gtatcgcggc gctgacccag     7860

cgtgcgctgg cgaccctggc gtctgcgccg atcaacgcga ccgcgaaagc gggtctgtct     7920

gaactggcga tgatggcggc gaaccgttct gcgtaatatt ttttaatatt atgaactgaa     7980

tttaagaggc tgcctcccga ttttatcgga aggcagcctt ttttataaag ctt            8033


<210> 6
<211> 8531
<212> DNA
<213> cassette


<220> 
<223> Gene cassette (G3)

<400> 6
cccgggtgtc tatactccag ttactcaata cgtaacaata atcagtttat cctaactata       60

gaatcgcatg agaagcgata acgtttcacc ataagcaata tattcattgc aacagtggaa      120

ttgccttatg cgtcaaggaa ggatagatca ttgacggact gagttcaaaa agagactcgt      180

ctaaaagatt ttaagaaagg tttcgatatg acccgtgaag ttgttgttgt ttctggtgtt      240

cgtaccgcga tcggcacctt cggtggttct ctgaaagacg ttgcgccggc ggaactgggt      300

gcgctggttg ttcgtgaagc gctggcgcgt gcgcaggttt ctggtgacga cgttggtcac      360

gttgttttcg gtaacgttat ccagaccgaa ccgcgtgaca tgtacctggg tcgtgttgcg      420

gcggttaacg gtggtgttac cgttaacgcg ccggcgctga ccgttaaccg tctgtgcggt      480

tctggtcttc aggcgatcgt ttctgcggcg cagaccatcc tgctgggtga caccgacgtt      540

gcgatcggtg gtggtgcgga atctatgtct cgtgcgccgt acctggcgcc ggcggcgcgt      600

tggggtgcgc gtatgggtga cgcgggtctg gttgacatga tgctgggtgc gctgcacgac      660

ccgttccacc gtatccacat gggtgttacc gcggaaaacg ttgcgaaaga atacgacatc      720

tctcgtaccc agcaggacga agcggcgctg gaatctcacc gtcgtgcgtc tgcggcgatc      780

aaagcgggtt acttcaaaga ccagatcgtt ccggttgttt ctaaaggtcg taaaggtgac      840

gttaccttcg acaccgacga acacgttcgt cacgacgcga ccatcgacga catgaccaaa      900

ctgcgtccgg ttttcgttaa agaaaacggc accgttaccg cgggtaactc ttctggtctg      960

aacgacgcgg cggcggcggt tgttatgatg gaacgtgcgg aagcggaacg tcgtggtctg     1020

aaaccgctgg cgcgtctggt ttcttacggt cacgcgggtg ttgacccgaa aaccatgggt     1080

atcggtccgg ttccggcgac caaaatcgcg ctggaacgtg cgggtcttca ggtttctgac     1140

ctggacgtta tcgaagcgaa cgaagcgttc gcggcgcagg cgtgcgcggt taccaaagcg     1200

ctgggtctgg acccggcgaa agttaacccg aacggttctg gtatctctct gggtcacccg     1260

atcggtgcga ccggtgcgct gatcaccgtt aaagcgctgc acgaactgaa ccgtgttcag     1320

ggtcgttacg cgctggttac catgtgcatc ggtggtggtc agggtatcgc ggcgatcttc     1380

gaacgtatct aatttaccaa cactactacg tataaggaga gcgttgttat gctgtacaaa     1440

ggtgacaccc tgtacctgga ctggctggaa gacggtatcg cggaactggt tttcgacgcg     1500

ccgggttctg ttaacaaact ggacaccgcg accgttgcgt ctctgggtga agcgatcggt     1560

gttctggaac agcagtctga cctgaaaggt ctgctgctgc gttctaacaa agcggcgttc     1620

atcgttggtg cggacatcac cgaatttctg tctctgttcc tggttccgga agaacagctg     1680

tctcagtggc tgcacttcgc gaactctgtt ttcaaccgtc tggaagacct gccggttccg     1740

accatcgcgg cggttaacgg ttacgcgctg ggtggtggtt gcgaatgcgt tctggcgacc     1800

gactaccgtc tggcgacccc ggacctgcgt atcggtctgc cggaaaccaa actgggtatc     1860

atgccgggtt tcggtggttc tgttcgtatg ccgcgtatgc tgggtgcgga ctctgcgctg     1920

gaaatcatcg cggcgggtaa agacgttggt gcggaccagg cgctgaaaat cggtctggtt     1980

gacggtgttg ttaaagcgga aaaactggtt gaaggtgcga aagcggttct gcgtcaggcg     2040

atcaacggtg acctggactg gaaagcgaaa cgtcagccga aactggaacc gctgaaactg     2100

tctaaaatcg aagcgaccat gtctttcacc atcgcgaaag gtatggttgc gcagaccgcg     2160

ggtaaacact acccggcgcc gatcaccgcg gttaaaacca tcgaagcggc ggcgcgtttc     2220

ggtcgtgaag aagcgctgaa cctggaaaac aaatctttcg ttccgctggc gcacaccaac     2280

gaagcgcgtg cgctggttgg tatcttcctg aacgaccagt acgttaaagg taaagcgaaa     2340

aaactgacca aagacgttga aaccccgaaa caggcggcgg ttctgggtgc gggtatcatg     2400

ggtggtggta tcgcgtacca gtctgcgtgg aaaggtgttc cggttgttat gaaagacatc     2460

aacgacaaat ctctgaccct gggtatgacc gaagcggcga aactgctgaa caaacagctg     2520

gaacgtggta aaatcgacgg tctgaaactg gcgggtgtta tctctaccat ccacccgacc     2580

ctggactacg cgggtttcga ccgtgttgac atcgttgttg aagcggttgt tgaaaacccg     2640

aaagttaaaa aagcggttct ggcggaaacc gaacagaaag ttcgtcagga caccgttctg     2700

gcgtctaaca cctctaccat cccgatctct gaactggcga acgcgctgga acgtccggaa     2760

aacttctgcg gtatgcactt cttcaacccg gttcaccgta tgccgctggt tgaaatcatc     2820

cgtggtgaaa aatcttctga cgaaaccatc gcgaaagttg ttgcgtgggc gtctaaaatg     2880

ggtaaaaccc cgatcgttgt taacgactgc cctggtttct tcgttaaccg tgttctgttc     2940

ccgtacttcg cgggtttctc tcagctgctg cgtgacggtg cggacttccg taaaatcgac     3000

aaagttatgg aaaaacagtt cggttggccg atgggtccgg cgtacctgct ggacgttgtt     3060

ggtatcgaca ccgcgcacca cgcgcaggcg gttatggcgg cgggtttccc gcagcgtatg     3120

cagaaagact accgtgacgc gatcgacgcg ctgttcgacg cgaaccgttt cggtcagaaa     3180

aacggtctgg gtttctggcg ttacaaagaa gactctaaag gtaaaccgaa aaaagaagaa     3240

gacgcggcgg ttgaagacct gctggcggaa gtttctcagc cgaaacgtga cttctctgaa     3300

gaagaaatca tcgcgcgtat gatgatcccg atggttaacg aagttgttcg ttgcctggaa     3360

gaaggtatca tcgcgacccc ggcggaagcg gacatggcgc tggtttacgg tctgggtttc     3420

ccgccgttcc acggtggtgc gttccgttgg ctggacaccc tgggttctgc gaaatacctg     3480

gacatggcgc agcagtacca gcacctgggt ccgctgtacg aagttccgga aggtctgcgt     3540

aacaaagcgc gtcacaacga accgtactac ccgccggttg aaccggcgcg tccggttggt     3600

gacctgaaaa ccgcgtaatt taccaacact actacgtata aggagagcgt tgttatgctg     3660

taccagtctg aaacccttca gctgcactgg ctggaaaacg gtatcgcgga actggttttc     3720

gacgcgccgg gttctgttaa caaactggac acccagaccg ttgcgaacct gggtgaagcg     3780

ctggttgttc tggaaaaaca gtctgaactg aaaggtctgc tgctgcgttc tgcgaaagcg     3840

gcgttcatcg ttggtgcgga catcaccgaa tttctgtctc tgttcaacga accgccggaa     3900

aaactgcacc agtggctggt tttcgcgaac gacatcttca accgtctgga agacctgccg     3960

gttccgacca tcgcggcgat caacggttac gcgctgggtg gtggttgcga atgcatcctg     4020

gcgaccgact tccgtgttgc gtctccggaa gcgcgtatcg gtctgccgga aaccaaactg     4080

ggtatcatgc cgggtttcgg tggttctgtt cgtctgccgc gtctgctggg tgcggactct     4140

gcgctggaaa tcatcgcggc gggtaaagac atcatcgcga aagacgcgct gaaagttggt     4200

ctggttgacg cggttgttgc gccggaaaaa ctggttgacg cggcgctgtc tatcctgaac     4260

caggcgatcg acggtaaact ggactggcag gcggcgcgtc gtccgaaact ggaaccgctg     4320

aaactgaacc cgaccgaagc ggcgatgtgc ttcaccatcg cgaaaggtat ggttatgcag     4380

gttgcgggta aacactaccc ggcgccgctg accgcggtta aaaccatcga agcggcggcg     4440

aaattcggtc gtgcggaagc gctgatcctg gaaaccaact ctttcgttcc gctggcgggt     4500

tctaacgaag cgcgtgcgct ggttggtatc ttcctgaacg accagtacgt taaaggtcag     4560

gcgaaaaaac tgtctaaagg tgtttctgcg ccgaaacagg cggcggttct gggtgcgggt     4620

atcatgggtg gtggtatcgc gtaccagtct gcgctgaaag gtgttccggt tatcatgaaa     4680

gacatcaacg acaaatctct gaccctgggt atgaacgaag cggcgaaact gctgaacaaa     4740

cagctggaac gtggtaaaat cgacggtctg aaaatggcga acatcctggc gaccatccag     4800

ccgaccctgg actacgcggg tatcgaacgt gcgcaggtta tcgttgaagc ggttgttgaa     4860

aacccgaaag ttaaagcggc ggttctggcg gaagttgaaa ccctgatcgg tgaagaaacc     4920

gttctggcgt ctaacacctc taccatcccg atcgaccagc tggcgaaatc tctgaaacgt     4980

ccggaaaact tctgcggtat gcacttcttc aacccggttc accgtatgcc gctggttgaa     5040

atcatccgtg gcaccaaaac ctctgacaaa accatcgcgg cggttgttgc gtacgcgacc     5100

cagatgggta aaaccccgat cgttgttaac gactgccctg gtttcttcgt taaccgtgtt     5160

ctgttcccgt acctggcggg tttcggtatg ctggttcgtg acggtgcgga cttccgtcag     5220

atcgacaaag ttatggaaaa acagttcggt tggccgatgg gtccggcgta cctgctggac     5280

gttgttggta tcgacaccgc gcaccacgcg caggcggtta tggcggttgg tttcccggaa     5340

cgtatgaaca aagactaccg tgacgcggtt gacgttatgt tcgacaacca gcgtttcggt     5400

cagaaaaacg gtcagggttt ctaccgttac acccaggaca ccaaaggtaa accgcgtaaa     5460

gaaaacgacg aacaggttga cgcgctgctg gcgcaggttt ctcagccgct tcagaaattc     5520

tctgacgacg acatcatcgc gcgtaccatg atcccgatga tcaacgaagt tgttcgttgc     5580

ctggaagaag gtatcatcgc gtctccggcg gaaggtgaca tggcgctggt ttacggtctg     5640

ggtttcccgc cgttccacgg tggtgttttc cgttacctgg acaccatcgg ttctgcgaac     5700

tacgttgaaa tggcgcagcg ttacacccac ctgggtgcgc tgtaccaggt tccgccgggt     5760

ctgcgtgcga aagcggaaca caacgaatct tactacccgg ttgcggcggc gctgctggac     5820

gtttctatct ctcagccggc gtaatttacc aacactacta cgtataagga gagcgttgtt     5880

atgtcttgcc cggcgtctcc gtctgcggcg gttgtttctg cgggtgcgct gtgcctgtgc     5940

gttgcgaccg ttctgctggc gaccggttct aacccgaccg cgctgtctac cgcgtctacc     6000

cgttctccga cctctctggt tcgtggtgtt gaccgtggtc tgatgcgtcc gaccaccgcg     6060

gcggcgctga ccaccatgcg tgaagttccg cagatggcgg aaggtttctc tggtgaagcg     6120

acctctgcgt gggcggcggc gggtccgcag tgggcggcgc cgctggttgc ggcggcgtct     6180

tctgcgctgg cgctgtggtg gtgggcggcg cgtcgttctg ttcgtcgtcc gctggcggcg     6240

ctggcggaac tgccgaccgc ggttacccac ctggcgccgc cgatggcgat gttcaccacc     6300

accgcgaaag ttatccagcc gaaaatccgt ggtttcatct gcaccaccac ccacccgatc     6360

ggttgcgaaa aacgtgttca ggaagaaatc gcgtacgcgc gtgcgcaccc gccgacctct     6420

ccgggtccga aacgtgttct ggttatcggt tgctctaccg gttacggtct gtctacccgt     6480

atcaccgcgg cgttcggtta ccaggcggcg accctgggtg ttttcctggc gggtccgccg     6540

accaaaggtc gtccggcggc ggcgggttgg tacaacaccg ttgcgttcga aaaagcggcg     6600

ctggaagcgg gtctgtacgc gcgttctctg aacggtgacg cgttcgactc taccaccaaa     6660

gcgcgtaccg ttgaagcgat caaacgtgac ctgggcaccg ttgacctggt tgtttactct     6720

atcgcggcgc cgaaacgtac cgacccggcg accggtgttc tgcacaaagc gtgcctgaaa     6780

ccgatcggtg cgacctacac caaccgtacc gttaacaccg acaaagcgga agttaccgac     6840

gtttctatcg aaccggcgtc tccggaagaa atcgcggaca ccgttaaagt tatgggtggt     6900

gaagactggg aactgtggat tcaggcgctg tctgaagcgg gtgttctggc ggaaggtgcg     6960

aaaaccgttg cgtactctta catcggtccg gaaatgacct ggccggttta ctggtctggc     7020

accatcggtg aagcgaaaaa agacgttgaa aaagcggcga aacgtatcac ccagcagtac     7080

ggttgcccgg cgtacccggt tgttgcgaaa gcgctggtta cccaggcgtc ttctgcgatc     7140

ccggttgttc cgctgtacat ctgcctgctg taccgtgtta tgaaagaaaa aggcacccac     7200

gaaggttgca tcgaacagat ggttcgtctg ctgaccacca aactgtaccc ggaaaacggt     7260

gcgccgatcg ttgacgaagc gggtcgtgtt cgtgttgacg actgggaaat ggcggaagac     7320

gttcagcagg cggttaaaga cctgtggtct caggtttcta ccgcgaacct gaaagacatc     7380

tctgacttcg cgggttacca gaccgaattt ctgcgtctgt tcggtttcgg tatcgacggt     7440

gttgactacg accagccggt tgacgttgaa gcggacctgc cgtctgcggc gcagcagtaa     7500

tttaccaaca ctactacgta taaggagagc gttgttatgt ctggtgcggc ggacgttgaa     7560

cgtgtttacg cggcgatgga agaagcggcg ggtctgctgg acgtttcttg cgcgcgtgaa     7620

aaaatctacc cgctgctgac cgttttccag gacaccctga ccgacggtgt tgttgttttc     7680

tctatggcgt ctggtcgtcg ttctaccgaa ctggacttct ctatctctgt tccggtttct     7740

cagggtgacc cgtacgcgac cgttgttaaa gaaggtctgt tccaggcgac cggttctccg     7800

gttgacgaac tgctggcgga caccgttgcg cacctgccgg tttctatgtt cgcgatcgac     7860

ggtgaagtta ccggtggttt caaaaaaacc tacgcgttct tcccgaccga cgacatgccg     7920

ggtgttgcgc agctggcggc gatcccgtct atgccggcgt ctgttgcgga aaacgcggaa     7980

ctgttcgcgc gttacggtct ggacaaagtt cagatgacct ctatggacta caaaaaacgt     8040

caggttaacc tgtacttctc tgacctgaaa caggaatacc ttcagccgga atctgttgtt     8100

gcgctggcgc gtgaactggg tctgcgtgtt ccgggtgaac tgggtctgga attttgcaaa     8160

cgttctttcg cggtttaccc gaccctgaac tgggacaccg gtaaaatcga ccgtctgtgc     8220

ttcgcggcga tctctaccga cccgaccctg gttccgtctg aagacgaacg tgacatcgaa     8280

atgttccgta actacgcgac caaagcgccg tacgcgtacg ttggtgaaaa acgtaccctg     8340

gtttacggtc tgaccctgtc ttctaccgaa gaatactaca aactgggtgc gtactaccac     8400

atcaccgaca tccagcgtca gctgctgaaa gcgttcgacg cgctggaaga ctaatatttt     8460

ttaatattat gaactgaatt taagaggctg cctcccgatt ttatcggaag gcagcctttt     8520

ttataggatc c                                                          8531


<210> 7
<211> 4723
<212> DNA
<213> plasmids


<220> 
<223> CRISRP_Loci-pUCZM-7

<400> 7
ccgggaattt gacccttatt ttgaccctct tttttcgagg gtataaaaaa tcctttcgat       60

tcaatatgtt acatatgagc atatttttta gggttatttt gcctttttgg cgagatatcc      120

ctttatttta ggggcaattc tatcttttgc ctctagttca ctgccgcaca ggcagcttag      180

aaaggagacc gaggtctcag ttcactgccg cacaggcagc ttagaaagga tcctctagac      240

tgcagaagct tataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt      300

gtttgtcggt gaacgctctc ctgagtagga caaatccgcc gggagcggat ttgaacgttg      360

cgaagcaacg gcccggaggg tggcgggcag gacgcccgcc ataaactgcc aggcatcaaa      420

ttaagcagaa ggccatcctg acggatggcc tttgtagaaa agatcaaagg atcttcttga      480

gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg      540

gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc      600

agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag      660

aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc      720

agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg      780

cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac      840

accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga      900

aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt      960

ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag     1020

cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg     1080

gcctttttac ggttcctggc cttttgctgg ccttttgctc attcaaatat gtatccgctc     1140

atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatggaaaaa     1200

aaaatcaccg gctataccac cgtggatatt agccagtggc atcgtaaaga acattttgaa     1260

gcgtttcaga gcgtggcgca gtgcacctat aaccagaccg tgcagctgga tatcaccgcg     1320

tttctgaaaa ccgtgaaaaa aaacaaacac aaattctacc cggcgtttat tcatattctg     1380

gcccgtctga tgaacgcgca tccggaattt cgtatggcca tgaaagatgg cgaactggtg     1440

atttgggata gcgtgcatcc gtgctatacc gtgtttcatg aacagaccga aacctttagc     1500

agcctgtgga gcgaatatca tgatgatttt cgccagttcc tgcatattta tagccaggat     1560

gtggcgtgct atggcgaaaa cctggcctat tttccgaaag gcttcatcga aaacatgttc     1620

tttgtgagcg cgaatccgtg ggtgagcttt accagcttcg atctgaacgt ggcgaacatg     1680

gataactttt ttgcgccggt gtttaccatg ggcaaatatt atacccaggg cgataaagtg     1740

ctgatgccgc tggccattca ggtgcatcat gcggtgtgtg atggctttca tgtgggccgt     1800

atgctgaacg aactgcagca gtattgtgat gaatggcagg gcggtgcgta ataactgtca     1860

gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg     1920

atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg     1980

ttccactgag cgtcgacggt accatgatta tcggcttctc aaaacatgga cagggcaaag     2040

ctgctccagc cataaaatac ctgctcgatc agcacagaga gggcagagaa aatcatattc     2100

ctgaagtggt gagaggtaat ccagacttaa ccgcatccat tatcgacagc ctgactttca     2160

aacatcacta tactagcgga gttctttctt tcgctgccga agatcatgtt tctgccgagc     2220

aggaaaaaga tattatcaac gatttcgaaa agctggcttt tgctggcctg caagcagacc     2280

agtttaatat cctttgggtg cgccattctc atgcgggtca tcatgaactt catttcatca     2340

ctccaaggat agaactttca tccaagaagt ccttcaacat caagcccccc ggtaagaata     2400

cccaaaagga atttgatgac ttgcggagtg ccatcaatgc caaatatggc tttgctgacc     2460

cagaagaccc gatgagagcg agaaacgtca aaacacctag ctatgaacta aaaatcgcta     2520

ggaatgtcct tagagagcaa aaagcgccct ctgaggacat aagacagctt attgatagcc     2580

ttttaactga aagagccgtc caaggcctga tacatgacag aaaaactctt ctcactaatg     2640

ttgaagagtt aggcttcgaa gttactcgcg caggcaagaa ttatattacc gtcaaagacc     2700

ctgaaagctc aaaacgattt cgtctcaaag gccaactcta tgaacaagac tttacctcta     2760

gccgaacgat tgaaacagca gcagctaaaa gaacaagaga ttattctcga cctgactcaa     2820

cagcagctag acaatttgag gaacaagtta aactccacat tcgaaaacga gctgaattca     2880

atagaaaacg atatcgagcg actgaaccgg agcttaggat ggaaattcct cctatctcgc     2940

tggctgatac cagtcgtgac cagcatcatg atattgtttg gcctgctctt gggcgcagaa     3000

gggataatca agctctatac ccaccagatc aaagctttgc aggaggaaaa agcagagatg     3060

gaagtgaccg tcgaacagct caacaaagca ggtgggaaga tgcaattcag ccattgcgga     3120

gaaaaagaaa ggctttgcat cagagtaaac acacgagaga tcttcgggat aaccagatat     3180

tatctgatcg tgaaagaacg gaaatagaca atgacggaat tggaacaggt tttactgaac     3240

gccttggaaa atttacagca gagattcgat caacaacaga aaagtttatt acacgcgcaa     3300

agcgaattac ggcagatgtt cgaaactacc tcaaccgaga atacttattt acgacagcaa     3360

gtagaaatct tggtcgagcg attgaacagt tttcccagat gaaaacctta agtcgaaagc     3420

tacaaaaacg gagaggtcgt tctcgctctc gataaacttg aaaaggacgc taattccgaa     3480

aagtttttta tctttctgat agtcgatttt agttggcgat ttccgataaa aaagccctga     3540

acttattgaa ctttcctata tttcaataag ttcaataagt tcagggcttt tttattcaaa     3600

aaaatcaatg ccttacgact ttaaaaacca cttttgggga gtgaaaaacc acttttgggg     3660

agtgaaaaac cacttttggg gagtgaaaaa ccacttcaaa aaatatatta agtggttaat     3720

aaagttgtaa tgagaaaaac actacaaata attaaagata gagccggcaa taaagatcaa     3780

aatttacatg caggtgaaat tatagatgga catttttcag accttaaaaa tccgcctagc     3840

ctagctgcac taaaactcta tgagttgctt attaaatacg cagcatataa tatagataaa     3900

aatatatggc ataatatttc tgtatcagaa atgcaaaaaa tcaaaggaat aaagaactat     3960

aactcaacag agctaactca attattaaaa gaacttagag ctgttgttat ggaatataga     4020

gcaaaagata gaactattat tactggtctt ttagatattg ctgcggtcat atctaaccca     4080

gacacaggat atctagagat acaatggaaa tttggagaaa gttttagaga tttaatagct     4140

aagtcagaat attgggcaat tgttgattat agaacaacgt tagagatgac gagccgatat     4200

tcccttcgtt tatatgaaat attagctctt agaataaatt taaaacacaa aaattcagat     4260

acatttactt tagccgagct tagaggaaaa ctgggagttc ctgaaggaaa attaaaaaga     4320

tggtctgatt taagagataa agcgatcaat gttgcggtaa aagaaattaa ccaacggact     4380

actttcgccg taacagcaca accaaaaaaa atgggaagat ccgttcaagc cgtagaattt     4440

aaatgggaac gaaaagagat taaacttcct caaagtatac cattaaaaac atctaaaagc     4500

attgtttttc cattagatgg aagtattaga tatactcaat gggaagatat tgcacgaaaa     4560

aatcttccta agcctttacc tgatttagat atggtagcac aacaatttag aaaatggtta     4620

aaaaataaaa acatttcatc aaacagtatt aatgttttgt ccatttttaa gaaattttgt     4680

gaaaaataca gaatagaatt taataattaa gaattcagta ctc                       4723


<210> 8
<211> 28
<212> DNA
<213> plasmids


<220> 
<223> CRISPR Plasmid Repeat Sequences

<400> 8
gttcactgcc gcacaggcag cttagaaa                                       28


<210> 9
<211> 2362
<212> DNA
<213> Zymomonas mobilis


<220> 
<223> Origen of replication 1 Zymomonas mobilis (Ori1)

<400> 9
cggaaaggtc atcctgaaaa ttctattcgt attctgatca accagatcgt gaatgatcgg      60

gcgatgagcc ggaaacttgt cccgtgatca gaaccggcgc agcctcttcc cttttatcta     120

tttcaaattc aggcctgcca cagcggagaa tcttcgctgt ggcttttttg cgtccaagac     180

cggagcccgc acaatgaccg cctcttcctc ttcagcgccc ctctcggatg cctctgtctt     240

ttctttagag cctgtctctg cgtcttttga acaaacgacg cccgtaacca gtccttttat     300

cgacaggcta ggccgtttca agcgtggcac ttatcgaacc atgttggttg aaacgggtat     360

gccatttgac agcgcaaaga ttatggcaga tcaggccgca gaacaacata aaagagagcg     420

cacattctct aaattaatgc ccttggccaa tggcgctcat cccgaaattt cggcctcaaa     480

taccaatatt gaggctacag cctctgcatc agatttcgac gaaatcgaat attttgacca     540

gatggtatca aacggcgttg atcctagaga tgccgcagat attgctcgca aaacagctat     600

tgcgagacgt cgagaccctc tcgaacgagc cattcgctat aaaacccgcc gtcaaactcc     660

tgatcctgtg catcgggatt cttatgaggt gggcgaacgc gagaagtcgg tctggaagcc     720

ggtcaatccg caagagattg gtgcctatct ggaagcggtt gatcaatatt cgatcaaaac     780

caagggctta agcgataagg ccgttcgtct tctaaagatg ctgtttcgga tggtcgactt     840

taaaactggc cgtctggagc cgaccctcga cacaatatgc gatcgtgtcg gttatgcccg     900

tgcgacggtc gtcaaattac tgcggcaatt acaggattta ggcttcatcc gctggatacg     960

ccggtcaatc aagatcaagg cggacggcaa aggcccccgc cgcaagcaga ccagtaacgc    1020

ctacggcttc ttgtctccca aggcttggcc agatttggcc agacaggttt tcgagcgtgt    1080

gatgcgccgg aggcatgcgc ctattcctga cgatatcgac cacgcccaag aggcggacaa    1140

agccgaaacc aaggctatcg ttgaaaactt gccgcccgaa gatttcgtca aagcaatatc    1200

gggcgataaa gagccaacag agttaacaga ttcgttaata cagctcgcca aatctatcga    1260

gcgggcggaa caacgggaac aagaagaaca ggctaagaag gcagcacagg ccggtcagga    1320

agaggttaaa acatcttccc aaacacctaa ggaggatacc tcaaaaaagc ccccaatctc    1380

gggcaatcaa cgcgagttca attcttatac tctatcctgt tatacttatt tatctaatag    1440

cgaccataca aaactaaaaa ccaaagaaga agataaagac tgcactgctc ctgctaccgc    1500

aggcagtgca gaccctattt atgatcgaaa acctgacagc aatcctttcc tctcaactct    1560

caaagctcgc ggttatcgtc ctgatcaaat caacgcttgc ctcgaaaaca gagataaacg    1620

gaaaaagaaa ggatggatgc ctctgtcacc cgatgaaacc gcccgcctcc ttattcaaac    1680

aaaacagatg tgcccataac aacgaccact atccttctca aagcatatcg tccgttaatc    1740

tctcggcgct gctcgcttcg ctcacaggct cggcgagaaa gaaatttgcc tttcggggga    1800

ctatctaatt cctaacaatt cgcattatcg ataatgtaat aaaaggctaa tctttaaaaa    1860

ccaaattaca gcgattacat catttctatg aatgtgtatt atacataata tgagaagtcg    1920

tgatttgatc aaagaagtta aaaaagccaa tattaccagc ttgacagata taaaataggc    1980

cttggttgat ttcgtgaaag aactggaaga ttgcacaatt cttctttctg gtgatatctt    2040

tgacccgacg gccctcagtc aatgagtgtg cgccgcattt ctgcccattc aatcagggcc    2100

tgcattgaag ggccgagagc gtatcccata ccgctgagct cgtattctac ctttggcggg    2160

acctgcggat agaccgttcg gagcacgatg cgatcctttt ccagttgctt gagctgctgg    2220

atcagcatct tctggttgat gccgtcgatc tgcttttcca gtgaggagaa gcggattggg    2280

ccttttgcgg caaacaactg gcagatgata acaatcttcc attttccttc gagtaccctg    2340

agcgcttctg tcgtcgcctc ag                                             2362


<210> 10
<211> 2801
<212> DNA
<213> Zymomonas mobilis


<220> 
<223> Origen of replication 2 Zymomonas mobilis (Ori2)

<400> 10
gcaccgcaaa ggacatgctg atcgcgagat cggcgcgcta ctcgacactg tcataacaga      60

tcacattcgg agatagctga tgaatactgc gcagaatcag actcttaaaa gagcgttttt     120

gtcatcagaa gccgcagcag ctgactcttc agctgactgc ggcttttcta tgtctgaaaa     180

agatatgcca gattttgaag aaatgacgcc agccagccct tttatcgaca ggctaggccg     240

tttcaagcgc ggcacttatc gaagcatgtt gatgcagacg gggctttctt atgaacgcgc     300

gaaaggcatg gcagacaatg cggcggaaga atttaaaaaa tcaaaagcaa agtcgaaatc     360

tttgccattg gccaagagcg aaatcgctga acttggggct tcagaagcaa cgattgaagt     420

aacagctcat caggtaacat tttctgaatc acaggcacaa ccagaacctt tcgatgctta     480

ctgctgcttg caacagttaa aagatcaggg cgtggacggt gccacagctg cgaagattgc     540

aaatcaggcc tacaagcatc acaaagaagc cgaaaatttt gacgaagcgg tatatttcaa     600

ggaattagtc gatactggca tagatgcggt gatggctgct aatatggctc gtaaaacagc     660

cgaagctaag cgccgcgacc cgctggaaag atacggtatc cgtttaaagc cgcgccgcac     720

gcctgatcct gttcaccgtg actcttatga tgttggcgag cgcgaaaata cggtctggaa     780

gccggtcaat ccgaatgaga tcggggctta tctggaagca gtcgatcaat attcaatcaa     840

gaccggcaat atcagtgata gagccgtccg gctgctcaag atgctgtttc ggatggtgga     900

cttcaagaca ggtcgattag agccgacgct tgatacaatc tgcgaatggg tcggctatgc     960

acgggcaacg gttgtccgct tgctaaaaca gcttcaagag caaggtttta tccgctggat    1020

acgccgttca atcaagatca agacggatgg ggctgggccc cgccgcaaac agaccagtaa    1080

cgcttacggt ttcttgtcgc ctaaatcttg gccggagatg gcaagaaagg ttttcgagcg    1140

cgtgatgcgc cggaagaatg cgccggtctc tgctgatctt gctcatgctc aagaagccga    1200

caaagtcgaa accggtgcca tgattgaaag ccagccgaat gacgactggg tgaaagaaat    1260

cttcgcacag aagcgcgcgc aacatccgac gagtgacgag cagaaaaaca aaaaagaaca    1320

ggccgatgat ctggcagaga ctcttgccag cttcgccaaa tctatcgagc gggcagaaca    1380

acgggaacaa gaagaacagg ccaagaaagc catttcggcc gagcaaaatg aggttaaaac    1440

accctacaaa acactcaagg aggacacctc aaaaacgccc caaatttcgg cagttgagcg    1500

cgagttcaat tttgatactc tatcccgtta tagttatttt aataataacc actccaatca    1560

atttaaaaac caaaaagaga ttaaggactg cactgctcct gctgccgcag gcagtgcaga    1620

cgttgttttg aaaacaaaac ctcaaaccga cactttccta aaaacccttc aaactcgcgg    1680

ttaccgtcaa gaccagacca acgcttgcct cgaaaacaga gataaacgga aaaagaatgg    1740

atggatgcct ctgtcagcgg acgaaaccgc ccgcctcctt atacaaacaa ggcagatgcg    1800

accgtgatac cgacagctat ccttctcaaa gcacaccgcc cattaatctc tcggcgctgc    1860

tcgcttcgct cacaggctcg gcgaaaaaga aatttgcctt ttggggggct attaacttct    1920

aacaactcgc attatcgaaa tgtctttaaa taggtttatt ctctaaaagc gtttcgacat    1980

ggcttttact gttatccatc atccaatctt cagtcggtct tgagcgatat ccaatcaaac    2040

gaacaattct atcccagtca gcttgtagat ctctttcaaa tataggctca tgatgggcta    2100

tccgattacg cagcatccga atttcaccca aatctctata gagactctgt ctctggattt    2160

taatctcttg ctgtggatca agattcggga acacgttcaa caaatagaga tcccaaagcc    2220

gtttatcatg gcgctttgtg aacatagcct gccagaatac aaaattcagt tctggtatta    2280

cctttccggt cgtttccgcc ttcaaacagg ctttgtttac atccttcgat gcgctgtatc    2340

ctgatttgac aactggaagg cttcgtcgga aaccttctga ccacggccac tgatctccat    2400

aggtatagga tagcgcttcc gccacagcat ttcgggtcgc tatttctgtt atatgaaggg    2460

gggctagcaa tgcgccggat agctgggcat tccacacata taaatccaga gcgacgagat    2520

catcttttcc caatgccctg actgtgtctt catatcttcc aagtcgggga gccgataagg    2580

tctttctgat agaggtaatc ttgtcttgaa ttgacagcat ggctacccat ttatatgatc    2640

aaccacatat tcgcctcggg tctttgttgg cttatgcctc cccctgagga caaaagatac    2700

aaaaaagacc tcgcttacgg cggggtcttt ttttatccat tttcctgtat tgtataaagt    2760

ctttgctagc ctgacggaaa acgtaatcct gcggttaatc g                        2801


<210> 11
<211> 2707
<212> DNA
<213> Zymomonas mobilis


<220> 
<223> Origen of replication 3 Zymomonas mobilis (Ori3)

<400> 11
atgattatcg gcttctcaaa acatggacag ggcaaagctg ctccagccat aaaatacctg      60

ctcgatcagc acagagaggg cagagaaaat catattcctg aagtggtgag aggtaatcca     120

gacttaaccg catccattat cgacagcctg actttcaaac atcactatac tagcggagtt     180

ctttctttcg ctgccgaaga tcatgtttct gccgagcagg aaaaagatat tatcaacgat     240

ttcgaaaagc tggcttttgc tggcctgcaa gcagaccagt ttaatatcct ttgggtgcgc     300

cattctcatg cgggtcatca tgaacttcat ttcatcactc caaggataga actttcatcc     360

aagaagtcct tcaacatcaa gccccccggt aagaataccc aaaaggaatt tgatgacttg     420

cggagtgcca tcaatgccaa atatggcttt gctgacccag aagacccgat gagagcgaga     480

aacgtcaaaa cacctagcta tgaactaaaa atcgctagga atgtccttag agagcaaaaa     540

gcgccctctg aggacataag acagcttatt gatagccttt taactgaaag agccgtccaa     600

ggcctgatac atgacagaaa aactcttctc actaatgttg aagagttagg cttcgaagtt     660

actcgcgcag gcaagaatta tattaccgtc aaagaccctg aaagctcaaa acgatttcgt     720

ctcaaaggcc aactctatga acaagacttt acctctagcc gaacgattga aacagcagca     780

gctaaaagaa caagagatta ttctcgacct gactcaacag cagctagaca atttgaggaa     840

caagttaaac tccacattcg aaaacgagct gaattcaata gaaaacgata tcgagcgact     900

gaaccggagc ttaggatgga aattcctcct atctcgctgg ctgataccag tcgtgaccag     960

catcatgata ttgtttggcc tgctcttggg cgcagaaggg ataatcaagc tctataccca    1020

ccagatcaga gctttgcagg aggaaaaagc agagatggaa gtgaccgtcg aacagctcaa    1080

caaagcaggt gggaagatgc aattcagcca ttgcggagaa aaagaaaggc tttgcatcag    1140

agtaaacaca cgagagatct tcgggataac cagatattat ctgatcgtga aagaacggaa    1200

atagacaatg acggaattgg aacaggtttt actgaacgcc ttggaaaatt tacagcagag    1260

attcgatcaa caacagaaaa gtttattaca cgcgcaaagc gaattacggc agatgttcga    1320

aactacctca accgagaata cttatttacg acagcaagta gaaatcttgg tcgagcgatt    1380

gaacagtttt cccagatgaa aaccttaagt cgaaagctac aaaaacggag aggtcgttct    1440

cgctctcgat aaacttgaaa aggacgctaa ttccgaaaag ttttttatct ttctgatagt    1500

cgattttagt tggcgatttc cgataaaaaa gccctgaact tattgaactt tcctatattt    1560

caataagttc aataagttca gggctttttt attcaaaaaa atcaatgcct tacgacttta    1620

aaaaccactt ttggggagtg aaaaaccact tttggggagt gaaaaaccac ttttggggag    1680

tgaaaaacca cttcaaaaaa tatattaagt ggttaataaa gttgtaatga gaaaaacact    1740

acaaataatt aaagatagag ccggcaataa agatcaaaat ttacatgcag gtgaaattat    1800

agatggacat ttttcagacc ttaaaaatcc gcctagccta gctgcactaa aactctatga    1860

gttgcttatt aaatacgcag catataatat agataaaaat atatggcata atatttctgt    1920

atcagaaatg caaaaaatca aaggaataaa gaactataac tcaacagagc taactcaatt    1980

attaaaagaa cttagagctg ttgttatgga atatagagca aaagatagaa ctattattac    2040

tggtctttta gatattgctg cggtcatatc taacccagac acaggatatc cagagataca    2100

atggaaattt ggagaaagtt ttagagattt aatagctaag tcagaatatt gggcaattgt    2160

tgattataga acaacgttag agatgacgag ccgatattcc cttcgtttat atgaaatatt    2220

agctcttaga ataaatttaa aacacaaaaa ttcagataca tttactttag ccgagcttag    2280

aggaaaactg ggagttcctg aaggaaaatt aaaaagatgg tctgatttaa gagataaagc    2340

gatcaatgtt gcggtaaaag aaattaacca acggactact ttcgccgtaa cagcacaacc    2400

aaaaaaaatg ggaagatccg ttcaagccgt agaatttaaa tgggaacgaa aagagattaa    2460

acttcctcaa agtataccat taaaaacatc taaaagcatt gtttttccat tagatggaag    2520

tattagatat actcaatggg aagatattgc acgaaaaaat cttcctaagc ctttacctga    2580

tttagatatg gtagcacaac aatttagaaa atggttaaaa aataaaaaca tttcatcaaa    2640

cagtattaat gttttgtcca tttttaagaa attttgtgaa aaatacagaa tagaatttaa    2700

taattaa                                                              2707


<210> 12
<211> 668
<212> DNA
<213> Escherichia coli


<220> 
<223> Origen of replication E. coli (pBR322)

<400> 12
gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg      60

caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact     120

ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg     180

tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg     240

ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac     300

tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca     360

cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga     420

gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc     480

ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct     540

gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg     600

agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct     660

tttgctca                                                              668


<210> 13
<211> 589
<212> DNA
<213> Escherichia coli


<220> 
<223> colE1

<400> 13
ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc      60

agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt     120

cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt     180

caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc     240

tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa     300

ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac     360

ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg     420

gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga     480

gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact     540

tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaa                 589


<210> 14
<211> 870
<212> DNA
<213> Escherichia coli


<220> 
<223> CamR

<400> 14
ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa      60

aaggaagagt atggaaaaaa aaatcaccgg ctataccacc gtggatatta gccagtggca     120

tcgtaaagaa cattttgaag cgtttcagag cgtggcgcag tgcacctata accagaccgt     180

gcagctggat atcaccgcgt ttctgaaaac cgtgaaaaaa aacaaacaca aattctaccc     240

ggcgtttatt catattctgg cccgtctgat gaacgcgcat ccggaatttc gtatggccat     300

gaaagatggc gaactggtga tttgggatag cgtgcatccg tgctataccg tgtttcatga     360

acagaccgaa acctttagca gcctgtggag cgaatatcat gatgattttc gccagttcct     420

gcatatttat agccaggatg tggcgtgcta tggcgaaaac ctggcctatt ttccgaaagg     480

cttcatcgaa aacatgttct ttgtgagcgc gaatccgtgg gtgagcttta ccagcttcga     540

tctgaacgtg gcgaacatgg ataacttttt tgcgccggtg tttaccatgg gcaaatatta     600

tacccagggc gataaagtgc tgatgccgct ggccattcag gtgcatcatg cggtgtgtga     660

tggctttcat gtgggccgta tgctgaacga actgcagcag tattgtgatg aatggcaggg     720

cggtgcgtaa taactgtcag accaagttta ctcatatata ctttagattg atttaaaact     780

tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat     840

cccttaacgt gagttttcgt tccactgagc                                      870


<210> 15
<211> 1068
<212> DNA
<213> Escherichia coli


<220> 
<223> AmpR

<400> 15
ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa      60

aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt     120

ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca     180

gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag     240

ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc     300

ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca     360

gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt     420

aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct     480

gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt     540

aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga     600

caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact     660

tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc     720

acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga     780

gcgtggttct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt     840

agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga     900

gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact     960

ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga    1020

taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagc                 1068


<210> 16
<211> 1005
<212> DNA
<213> Escherichia coli


<220> 
<223> KanR

<400> 16
ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa      60

aaggaagagt atgattgaac aggatggcct gcatgcgggt agcccggcag cgtgggtgga     120

acgtctgttt ggctatgatt gggcgcagca gaccattggc tgctctgatg cggcggtgtt     180

tcgtctgagc gcgcagggtc gtccggtgct gtttgtgaaa accgatctga gcggtgcgct     240

gaacgagctg caggatgaag cggcgcgtct gagctggctg gccaccaccg gtgttccgtg     300

tgcggcggtg ctggatgtgg tgaccgaagc gggccgtgat tggctgctgc tgggcgaagt     360

gccgggtcag gatctgctgt ctagccatct ggcgccggca gaaaaagtga gcattatggc     420

ggatgccatg cgtcgtctgc ataccctgga cccggcgacc tgtccgtttg atcatcaggc     480

gaaacatcgt attgaacgtg cgcgtacccg tatggaagcg ggcctggtgg atcaggatga     540

tctggatgaa gaacatcagg gcctggcacc ggcagagctg tttgcgcgtc tgaaagcgag     600

catgccggat ggcgaagatc tggtggtgac ccatggtgat gcgtgcctgc cgaacattat     660

ggtggaaaat ggccgtttta gcggctttat tgattgcggc cgtctgggcg tggcggatcg     720

ttatcaggat attgcgctgg ccacccgtga tattgcggaa gaactgggcg gcgaatgggc     780

ggatcgtttt ctggtgctgt atggcattgc ggcaccggat agccagcgta ttgcgtttta     840

tcgtctgctg gatgaatttt tctaataact gtcagaccaa gtttactcat atatacttta     900

gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa     960

tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagc                    1005


<210> 17
<211> 792
<212> DNA
<213> Escherichia coli


<220> 
<223> TetR

<400> 17
atgagggaag cggtgatcgc cgaagtatcg actcaactat cagaggtagt tggcgtcatc      60

gagcgccatc tcgaaccgac gttgctggcc gtacatttgt acggctccgc agtggatggc     120

ggcctgaagc cacacagtga tattgatttg ctggttacgg tgaccgtaag gcttgatgaa     180

acaacgcggc gagctttgat caacgacctt ttggaaactt cggcttcccc tggagagagc     240

gagattctcc gcgctgtaga agtcaccatt gttgtgcacg acgacatcat tccgtggcgt     300

tatccagcta agcgcgaact gcaatttgga gaatggcagc gcaatgacat tcttgcaggt     360

atcttcgagc cagccacgat cgacattgat ctggctatct tgctgacaaa agcaagagaa     420

catagcgttg ccttggtagg tccagcggcg gaggaactct ttgatccggt tcctgaacag     480

gatctatttg aggcgctaaa tgaaacctta acgctatgga actcgccgcc cgactgggct     540

ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc agtaaccggc     600

aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat     660

cagcccgtca tacttgaagc tagacaggct tatcttggac aagaagaaga tcgcttggcc     720

tcgcgcgcag atcagttgga agaatttgtc cactacgtga aaggcgagat caccaaggta     780

gtcggcaaat aa                                                         792


<210> 18
<211> 792
<212> DNA
<213> Escherichia coli


<220> 
<223> SpectinomycinR

<400> 18
atgagggaag cggtgatcgc cgaagtatcg actcaactat cagaggtagt tggcgtcatc      60

gagcgccatc tcgaaccgac gttgctggcc gtacatttgt acggctccgc agtggatggc     120

ggcctgaagc cacacagtga tattgatttg ctggttacgg tgaccgtaag gcttgatgaa     180

acaacgcggc gagctttgat caacgacctt ttggaaactt cggcttcccc tggagagagc     240

gagattctcc gcgctgtaga agtcaccatt gttgtgcacg acgacatcat tccgtggcgt     300

tatccagcta agcgcgaact gcaatttgga gaatggcagc gcaatgacat tcttgcaggt     360

atcttcgagc cagccacgat cgacattgat ctggctatct tgctgacaaa agcaagagaa     420

catagcgttg ccttggtagg tccagcggcg gaggaactct ttgatccggt tcctgaacag     480

gatctatttg aggcgctaaa tgaaacctta acgctatgga actcgccgcc cgactgggct     540

ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc agtaaccggc     600

aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat     660

cagcccgtca tacttgaagc tagacaggct tatcttggac aagaagaaga tcgcttggcc     720

tcgcgcgcag atcagttgga agaatttgtc cactacgtga aaggcgagat caccaaggta     780

gtcggcaaat aa                                                         792


<210> 19
<211> 147
<212> DNA
<213> Zymomonas mobilis


<220> 
<223> Leader sequence in the crispr plasmid

<400> 19
aatttgaccc ttattttgac cctctttttt cgagggtata aaaaatcctt tcgattcaat     60

atgttacata tgagcatatt ttttagggtt attttgcctt tttggcgaga tatcccttta    120

ttttaggggc aattctatct tttgcct                                        147


<210> 20
<211> 202
<212> DNA
<213> Escherichia coli


<220> 
<223> Terminator

<400> 20
ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg     60

aacgctctcc tgagtaggac aaatccgccg ggagcggatt tgaacgttgc gaagcaacgg    120

cccggagggt ggcgggcagg acgcccgcca taaactgcca ggcatcaaat taagcagaag    180

gccatcctga cggatggcct tt                                             202


<210> 21
<211> 417
<212> DNA
<213> Zymomonas mobilis


<220> 
<223> Lambda red gen 1

<400> 21
atggatatta atactgaaac tgagatcaag caaaagcatt cactaacccc ctttcctgtt      60

ttcctaatca gcccggcatt tcgcgggcga tattttcaca gctatttcag gagttcagcc     120

atgaacgctt attacattca ggatcgtctt gaggctcaga gctgggcgcg tcactaccag     180

cagctcgccc gtgaagagaa agaggcagaa ctggcagacg acatggaaaa aggcctgccc     240

cagcacctgt ttgaatcgct atgcatcgat catttgcaac gccacggggc cagcaaaaaa     300

tccattaccc gtgcgtttga tgacgatgtt gagtttcagg agcgcatggc agaacacatc     360

cggtacatgg ttgaaaccat tgctcaccac caggttgata ttgattcaga ggtataa        417


<210> 22
<211> 786
<212> DNA
<213> Zymomonas mobilis


<220> 
<223> Lambda red gen 2

<400> 22
atgagtactg cactcgcaac gctggctggg aagctggctg aacgtgtcgg catggattct      60

gtcgacccac aggaactgat caccactctt cgccagacgg catttaaagg tgatgccagc     120

gatgcgcagt tcatcgcatt actgatcgtt gccaaccagt acggccttaa tccgtggacg     180

aaagaaattt acgcctttcc tgataagcag aatggcatcg ttccggtggt gggcgttgat     240

ggctggtccc gcatcatcaa tgaaaaccag cagtttgatg gcatggactt tgagcaggac     300

aatgaatcct gtacatgccg gatttaccgc aaggaccgta atcatccgat ctgcgttacc     360

gaatggatgg atgaatgccg ccgcgaacca ttcaaaactc gcgaaggcag agaaatcacg     420

gggccgtggc agtcgcatcc caaacggatg ttacgtcata aagccatgat tcagtgtgcc     480

cgtctggcct tcggatttgc tggtatctat gacaaggatg aagccgagcg cattgtcgaa     540

aatactgcat acactgcaga acgtcagccg gaacgcgaca tcactccggt taacgatgaa     600

accatgcagg agattaacac tctgctgatc gccctggata aaacatggga tgacgactta     660

ttgccgctct gttcccagat atttcgccgc gacattcgtg catcgtcaga actgacacag     720

gccgaagcag taaaagctct tggattcctg aaacagaaag ccgcagagca gaaggtggca     780

gcatga                                                                786


<210> 23
<211> 681
<212> DNA
<213> Zymomonas mobilis


<220> 
<223> Lambda red gen 3

<400> 23
atgacaccgg acattatcct gcagcgtacc gggatcgatg tgagagctgt cgaacagggg      60

gaagatgcgt ggcacaaatt acggctcggc gtcatcaccg cttcagaagt tcacaacgtg     120

atagcaaaac cccgctccgg aaagaagtgg cctgacatga aaatgtccta cttccacacc     180

ctgcttgctg aggtttgcac cggtgtggct ccggaagtta acgctaaagc actggcctgg     240

ggaaaacagt acgagaacga cgccagaacc ctgtttgaat tcacttccgg cgtgaatgtt     300

actgaatccc cgatcatcta tcgcgacgaa agtatgcgta ccgcctgctc tcccgatggt     360

ttatgcagtg acggcaacgg ccttgaactg aaatgcccgt ttacctcccg ggatttcatg     420

aagttccggc tcggtggttt cgaggccata aagtcagctt acatggccca ggtgcagtac     480

agcatgtggg tgacgcgaaa aaatgcctgg tactttgcca actatgaccc gcgtatgaag     540

cgtgaaggcc tgcattatgt cgtgattgag cgggatgaaa agtacatggc gagttttgac     600

gagatcgtgc cggagttcat cgaaaaaatg gacgaggcac tggctgaaat tggttttgta     660

tttggggagc aatggcgatg a                                               681


<210> 24
<211> 4107
<212> DNA
<213> Streptococcus pyogenes


<220> 
<223> Cas9

<400> 24
atggataaga aatactcaat aggcttagat atcggcacaa atagcgtcgg atgggcggtg       60

atcactgatg aatataaggt tccgtctaaa aagttcaagg ttctgggaaa tacagaccgc      120

cacagtatca aaaaaaatct tataggggct cttttatttg acagtggaga gacagcggaa      180

gcgactcgtc tcaaacggac agctcgtaga aggtatacac gtcggaagaa tcgtatttgt      240

tatctacagg agattttttc aaatgagatg gcgaaagtag atgatagttt ctttcatcga      300

cttgaagagt cttttttggt ggaagaagac aagaagcatg aacgtcatcc tatttttgga      360

aatatagtag atgaagttgc ttatcatgag aaatatccaa ctatctatca tctgcgaaaa      420

aaattggtag attctactga taaagcggat ttgcgcttaa tctatttggc cttagcgcat      480

atgattaagt ttcgtggtca ttttttgatt gagggagatt taaatcctga taatagtgat      540

gtggacaaac tatttatcca gttggtacaa acctacaatc aattatttga agaaaaccct      600

attaacgcaa gtggagtaga tgctaaagcg attctttctg cacgattgag taaatcaaga      660

cgattagaaa atctcattgc tcagctcccc ggtgagaaga aaaatggctt atttgggaat      720

ctcattgctt tgtcattggg tttgacccct aattttaaat caaattttga tttggcagaa      780

gatgctaaat tacagctttc aaaagatact tacgatgatg atttagataa tttattggcg      840

caaattggag atcaatatgc tgatttgttt ttggcagcta agaatttatc agatgctatt      900

ttactttcag atatcctaag agtaaatact gaaataacta aggctcccct atcagcttca      960

atgattaaac gctacgatga acatcatcaa gacttgactc ttttaaaagc tttagttcga     1020

caacaacttc cagaaaagta taaagaaatc ttttttgatc aatcaaaaaa cggatatgca     1080

ggttatattg atgggggagc tagccaagaa gaattttata aatttatcaa accaatttta     1140

gaaaaaatgg atggtactga ggaattattg gtgaaactaa atcgtgaaga tttgctgcgc     1200

aagcaacgga cctttgacaa cggctctatt ccccatcaaa ttcacttggg tgagctgcat     1260

gctattttga gaagacaaga agacttttat ccatttttaa aagacaatcg tgagaagatt     1320

gaaaaaatct tgacttttcg aattccttat tatgttggtc cattggcgcg tggcaatagt     1380

cgttttgcat ggatgactcg gaagtctgaa gaaacaatta ccccatggaa ttttgaagaa     1440

gttgtcgata aaggtgcttc agctcaatca tttattgaac gcatgacaaa ctttgataaa     1500

aatcttccaa atgaaaaagt actaccaaaa catagtttgc tttatgagta ttttacggtt     1560

tataacgaat tgacaaaggt caaatatgtt actgaaggaa tgcgaaaacc agcatttctt     1620

tcaggtgaac agaagaaagc cattgttgat ttactcttca aaacaaatcg aaaagtaacc     1680

gttaagcaat taaaagaaga ttatttcaaa aaaatagaat gttttgatag tgttgaaatt     1740

tcaggagttg aagatagatt taatgcttca ttaggtacct accatgattt gctaaaaatt     1800

attaaagata aagatttttt ggataatgaa gaaaatgaag atatcttaga ggatattgtt     1860

ttaacattga ccttatttga agatagggag atgattgagg aaagacttaa aacatatgct     1920

cacctctttg atgataaggt gatgaaacag cttaaacgtc gccgttatac tggttgggga     1980

cgtttgtctc gaaaattgat taatggtatt agggataagc aatctggcaa aacaatatta     2040

gattttttga aatcagatgg ttttgccaat cgcaatttta tgcagctgat ccatgatgat     2100

agtttgacat ttaaagaaga cattcaaaaa gcacaagtgt ctggacaagg cgatagttta     2160

catgaacata ttgcaaattt agctggtagc cctgctatta aaaaaggtat tttacagact     2220

gtaaaagttg ttgatgaatt ggtcaaagta atggggcggc ataagccaga aaatatcgtt     2280

attgaaatgg cacgtgaaaa tcagacaact caaaagggcc agaaaaattc gcgagagcgt     2340

atgaaacgaa tcgaagaagg tatcaaagaa ttaggaagtc agattcttaa agagcatcct     2400

gttgaaaata ctcaattgca aaatgaaaag ctctatctct attatctcca aaatggaaga     2460

gacatgtatg tggaccaaga attagatatt aatcgtttaa gtgattatga tgtcgatcac     2520

attgttccac aaagtttcct taaagacgat tcaatagaca ataaggtctt aacgcgttct     2580

gataaaaatc gtggtaaatc ggataacgtt ccaagtgaag aagtagtcaa aaagatgaaa     2640

aactattgga gacaacttct aaacgccaag ttaatcactc aacgtaagtt tgataattta     2700

acgaaagctg aacgtggagg tttgagtgaa cttgataaag ctggttttat caaacgccaa     2760

ttggttgaaa ctcgccaaat cactaagcat gtggcacaaa ttttggatag tcgcatgaat     2820

actaaatacg atgaaaatga taaacttatt cgagaggtta aagtgattac cttaaaatct     2880

aaattagttt ctgacttccg aaaagatttc caattctata aagtacgtga gattaacaat     2940

taccatcatg cccatgatgc gtatctaaat gccgtcgttg gaactgcttt gattaagaaa     3000

tatccaaaac ttgaatcgga gtttgtctat ggtgattata aagtttatga tgttcgtaaa     3060

atgattgcta agtctgagca agaaataggc aaagcaaccg caaaatattt cttttactct     3120

aatatcatga acttcttcaa aacagaaatt acacttgcaa atggagagat tcgcaaacgc     3180

cctctaatcg aaactaatgg ggaaactgga gaaattgtct gggataaagg gcgagatttt     3240

gccacagtgc gcaaagtatt gtccatgccc caagtcaata ttgtcaagaa aacagaagta     3300

cagacaggcg gattctccaa ggagtcaatt ttaccaaaaa gaaattcgga caagcttatt     3360

gctcgtaaaa aagactggga tccaaaaaaa tatggtggtt ttgatagtcc aacggtagct     3420

tattcagtcc tagtggttgc taaggtggaa aaagggaaat cgaagaagtt aaaatccgtt     3480

aaagagttac tagggatcac aattatggaa agaagttcct ttgaaaaaaa tccgattgac     3540

tttttagaag ctaaaggata taaggaagtt aaaaaagact taatcattaa actacctaaa     3600

tatagtcttt ttgagttaga aaacggtcgt aaacggatgc tggctagtgc cggagaatta     3660

caaaaaggaa atgagctggc tctgccaagc aaatatgtga attttttata tttagctagt     3720

cattatgaaa agttgaaggg tagtccagaa gataacgaac aaaaacaatt gtttgtggag     3780

cagcataagc attatttaga tgagattatt gagcaaatca gtgaattttc taagcgtgtt     3840

attttagcag atgccaattt agataaagtt cttagtgcat ataacaaaca tagagacaaa     3900

ccaatacgtg aacaagcaga aaatattatt catttattta cgttgacgaa tcttggagct     3960

cccgctgctt ttaaatattt tgatacaaca attgatcgta aacgatatac gtctacaaaa     4020

gaagttttag atgccactct tatccatcaa tccatcactg gtctttatga aacacgcatt     4080

gatttgagtc agctaggagg tgactaa                                         4107


<210> 25
<211> 36
<212> DNA
<213> Synthetic plasmid


<220> 
<223> Pyruvate Decarboxylase_sgRNA_FW - BsaI

<400> 25
gaaatcaatg caggtgaact gaagaaagcc gctccg                               36


<210> 26
<211> 36
<212> DNA
<213> Synthetic plasmid


<220> 
<223> Pyruvate Decarboxylase_sgRNA_RV - Bsal

<400> 26
gaaccggagc ggctttcttc agttcacctg cattga                               36


<210> 27
<211> 27
<212> DNA
<213> Synthetic plasmid


<220> 
<223> Pyruvate Decarboxylase_HDR_FW_1 - SaII

<400> 27
cgtcgacgtt ggttatgtcc tataatc                                        27


<210> 28
<211> 30
<212> DNA
<213> Synthetic plasmid


<220> 
<223> Pyruvate Decarboxylase_HDR_RV_1 - Xhol/Sacl

<400> 28
ctcgaggagc tctgcttact ccatatattc                                     30


<210> 29
<211> 31
<212> DNA
<213> Synthetic plasmid


<220> 
<223> Pyruvate Decarboxylase_HDR_FW_2 -SacI/Xhol

<400> 29
gagctcctcg agtttttaaa taaacttaga g                                   31


<210> 30
<211> 23
<212> DNA
<213> Synthetic plasmid


<220> 
<223> Pyruvate Decarboxylase_HDR_RV_2 - KpNI

<400> 30
tggtacctta aggcactgac ttc                                            23


<210> 31
<211> 36
<212> DNA
<213> Synthetic plasmid


<220> 
<223> Lactatedehydrogenase_sgRNA_FW - Bsal

<400> 31
gaaagaaaca attttaagtc gtgttgaaaa aggcga                               36


<210> 32
<211> 36
<212> DNA
<213> Synthetic plasmid


<220> 
<223> Lactatedehydrogenase_sgRNA_RV - Bsal

<400> 32
gaactcgcct ttttcaacac gacttaaaat tgtttc                               36


<210> 33
<211> 27
<212> DNA
<213> Synthetic plasmid


<220> 
<223> Lactatedehydrogenase_HDR_FW_1 - SaII

<400> 33
cgtcgacgaa agatagctat tattatc                                        27


<210> 34
<211> 32
<212> DNA
<213> Synthetic plasmid


<220> 
<223> Lactatedehydrogenase_HDR_RV_1 - Xhol/Sacl

<400> 34
ctcgaggagc tctatatcga ccttttattt tc                                   32


<210> 35
<211> 29
<212> DNA
<213> Synthetic plasmid


<220> 
<223> Lactatedehydrogenase_HDR_FW_2 - SacI/Xhol

<400> 35
gagctcctcg agttaagctg ctataaata                                      29


<210> 36
<211> 24
<212> DNA
<213> Synthetic plasmid


<220> 
<223> Lactatedehydrogenase_HDR_RV_2 - KpNI

<400> 36
tggtacctct gtcgccagat cgac                                           24


<210> 37
<211> 36
<212> DNA
<213> Synthetic plasmid


<220> 
<223> Phosphoenolpyruvatecarboxylase_sgRNA_FW - Bsal

<400> 37
gaaaatgaaa tttttggccc tgaatccatt acgact                               36


<210> 38
<211> 36
<212> DNA
<213> Synthetic plasmid


<220> 
<223> Phosphoenolpyruvatecarboxylase_sgRNA_RV - Bsal

<400> 38
gaacagtcgt aatggattca gggccaaaaa tttcat                               36


<210> 39
<211> 22
<212> DNA
<213> Synthetic plasmid


<220> 
<223> Phosphoenolpyruvatecarboxylase_HDR_FW_1 - SaII

<400> 39
cgtcgacttt atcgggaagc tg                                             22


<210> 40
<211> 28
<212> DNA
<213> Synthetic plasmid


<220> 
<223> Phosphoenolpyruvatecarboxylase_HDR_RV_1 - Xhol/Sacl

<400> 40
ctcgaggagc tcaaaccatc cctttgcc                                       28


<210> 41
<211> 33
<212> DNA
<213> Synthetic plasmid


<220> 
<223> Phosphoenolpyruvatecarboxylase_HDR_FW_2 - SacI/Xhol

<400> 41
gagctcctcg agtacatcag ccctatttta aag                                  33


<210> 42
<211> 24
<212> DNA
<213> Synthetic plasmid


<220> 
<223> Phosphoenolpyruvatecarboxylase_HDR_RV_2 - KpNI

<400> 42
tggtaccccg ccttgggctg tctg                                           24


<210> 43
<211> 33
<212> DNA
<213> Synthetic plasmid


<220> 
<223> KnockIN_G1_FW - SacI

<400> 43
gtcatgagct catggcgctg gtttctatgc gtc                                  33


<210> 44
<211> 32
<212> DNA
<213> Synthetic plasmid


<220> 
<223> KnockIN_G1_RV - Xhol

<400> 44
cagtactcga gttatttacg cggggtgtag tc                                   32


<210> 45
<211> 33
<212> DNA
<213> Synthetic plasmid


<220> 
<223> KnockIN_G2_FW - Sac

<400> 45
gtcatgagct catgaaatct gttaccatcc tgg                                  33


<210> 46
<211> 31
<212> DNA
<213> Synthetic plasmid


<220> 
<223> KnockIN_G2_RV - Xhol

<400> 46
cagtactcga gttacgcaga acggttcgcc g                                   31


<210> 47
<211> 33
<212> DNA
<213> Synthetic plasmid


<220> 
<223> KnockIN_G3_FW - SacI

<400> 47
gtcatgagct catgacccgt gaagttgttg ttg                                  33


<210> 48
<211> 34
<212> DNA
<213> Synthetic plasmid


<220> 
<223> KnockIN_G3_RV - Xhol

<400> 48
cagtactcga gttagtcttc cagcgcgtcg aacg                                 34


<210> 49
<211> 25
<212> DNA
<213> Synthetic plasmid


<220> 
<223> SequencingPrimer_ ORF_FW

<400> 49
cttcctaagc ctttacctga tttag                                          25


<210> 50
<211> 20
<212> DNA
<213> Synthetic plasmid


<220> 
<223> SequencingPrimer_ ORF_RV

<400> 50
cgactgagcc tttcgtttta                                                20


<210> 51
<211> 24
<212> DNA
<213> Synthetic plasmid


<220> 
<223> SequencingPrimer_ HDR_FW

<400> 51
tcatgaccaa aatcccttaa cgtg                                           24


<210> 52
<211> 24
<212> DNA
<213> Synthetic plasmid


<220> 
<223> SequencingPrimer_HDR _RV

<400> 52
ccatgttttg agaagccgat aatc                                           24


