                         SEQUENCE LISTING

<110>  Surrozen, Inc.
       Li, Yang
       Tu, Shengjiang
       Lee, Sungjin
       Yeh, Wen-Chen
 
<120>  MODULATION OF WNT SIGNALLING IN OCULAR DISORDERS

<130>  SRZN-013/01WO

<150>  US 62/803,835
<151>  2019-02-11

<160>  8     

<170>  PatentIn version 3.5

<210>  1
<211>  358
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Made in lab - synthesized WNT agonist

<400>  1

Met Asp Met Arg Val Pro Ala Gln Leu Leu Gly Leu Leu Leu Leu Trp 
1               5                   10                  15      


Leu Arg Gly Ala Arg Cys Asp Val Gln Leu Val Glu Ser Gly Gly Gly 
            20                  25                  30          


Leu Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Thr Ser Ser Ala 
        35                  40                  45              


Asn Ile Asn Ser Ile Glu Thr Leu Gly Trp Tyr Arg Gln Ala Pro Gly 
    50                  55                  60                  


Lys Gln Arg Glu Leu Ile Ala Asn Met Arg Gly Gly Gly Tyr Met Lys 
65                  70                  75                  80  


Tyr Ala Gly Ser Leu Lys Gly Arg Phe Thr Met Ser Thr Glu Ser Ala 
                85                  90                  95      


Lys Asn Thr Met Tyr Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr 
            100                 105                 110         


Ala Val Tyr Tyr Cys Tyr Val Lys Leu Arg Asp Asp Asp Tyr Val Tyr 
        115                 120                 125             


Arg Gly Gln Gly Thr Gln Val Thr Val Ser Ser Gly Gly Ser Gly Ser 
    130                 135                 140                 


Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
145                 150                 155                 160 


Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Gly Ile Ser Ser Tyr 
                165                 170                 175     


Leu Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
            180                 185                 190         


Tyr Ala Ala Ser Asn Leu Leu Gly Gly Val Pro Ser Arg Phe Ser Gly 
        195                 200                 205             


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
    210                 215                 220                 


Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Thr Tyr Ser Thr Pro Trp 
225                 230                 235                 240 


Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg Thr Val Ala Ala 
                245                 250                 255     


Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly 
            260                 265                 270         


Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala 
        275                 280                 285             


Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln 
    290                 295                 300                 


Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser 
305                 310                 315                 320 


Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val Tyr 
                325                 330                 335     


Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys Ser 
            340                 345                 350         


Phe Asn Arg Gly Glu Cys 
        355             


<210>  2
<211>  470
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Made in lab - synthesized WNT agonist

<400>  2

Met Asp Met Arg Val Pro Ala Gln Leu Leu Gly Leu Leu Leu Leu Trp 
1               5                   10                  15      


Leu Arg Gly Ala Arg Cys Glu Val Gln Leu Val Glu Ser Gly Gly Gly 
            20                  25                  30          


Leu Val Lys Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly 
        35                  40                  45              


Phe Asn Phe Gly Ile Tyr Ser Met Thr Trp Val Arg Gln Ala Pro Gly 
    50                  55                  60                  


Lys Gly Leu Glu Trp Ile Ser Tyr Ile Ser Gly Asp Ser Gly Tyr Thr 
65                  70                  75                  80  


Asn Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asp 
                85                  90                  95      


Ser Lys Asn Thr Leu Tyr Leu Gln Met Asn Ser Leu Lys Thr Glu Asp 
            100                 105                 110         


Thr Ala Val Tyr Tyr Cys Ala Arg Val Gly Pro Gly Gly Trp Phe Asp 
        115                 120                 125             


Pro Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Ala Ser Thr Lys 
    130                 135                 140                 


Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly 
145                 150                 155                 160 


Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro 
                165                 170                 175     


Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr 
            180                 185                 190         


Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val 
        195                 200                 205             


Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn 
    210                 215                 220                 


Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys Lys Val Glu Pro 
225                 230                 235                 240 


Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu 
                245                 250                 255     


Ala Ala Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp 
            260                 265                 270         


Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp 
        275                 280                 285             


Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly 
    290                 295                 300                 


Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn 
305                 310                 315                 320 


Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp 
                325                 330                 335     


Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Gly 
            340                 345                 350         


Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu 
        355                 360                 365             


Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn 
    370                 375                 380                 


Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile 
385                 390                 395                 400 


Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr 
                405                 410                 415     


Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys 
            420                 425                 430         


Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys 
        435                 440                 445             


Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu 
    450                 455                 460                 


Ser Leu Ser Pro Gly Lys 
465                 470 


<210>  3
<211>  360
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Made in lab - synthesized WNT agonist

<400>  3

Met Asp Met Arg Val Pro Ala Gln Leu Leu Gly Leu Leu Leu Leu Trp 
1               5                   10                  15      


Leu Arg Gly Ala Arg Cys Asp Val Gln Leu Val Glu Ser Gly Gly Gly 
            20                  25                  30          


Leu Val Gln Ala Gly Gly Ser Leu Arg Leu Ala Cys Ala Gly Ser Gly 
        35                  40                  45              


Arg Ile Phe Ala Ile Tyr Asp Ile Ala Trp Tyr Arg His Pro Pro Gly 
    50                  55                  60                  


Asn Gln Arg Glu Leu Val Ala Met Ile Arg Pro Val Val Thr Glu Ile 
65                  70                  75                  80  


Asp Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asn Asn 
                85                  90                  95      


Ala Met Lys Thr Val Tyr Leu Gln Met Asn Asn Leu Lys Pro Glu Asp 
            100                 105                 110         


Thr Ala Val Tyr Tyr Cys Asn Ala Lys Arg Pro Trp Gly Ser Arg Asp 
        115                 120                 125             


Glu Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser Gly Ser Gly 
    130                 135                 140                 


Gly Ser Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser 
145                 150                 155                 160 


Val Gly Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Gly Ile Ser 
                165                 170                 175     


Ser Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu 
            180                 185                 190         


Leu Ile Tyr Ala Ala Ser Asn Leu Leu Gly Gly Val Pro Ser Arg Phe 
        195                 200                 205             


Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu 
    210                 215                 220                 


Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Thr Tyr Ser Thr 
225                 230                 235                 240 


Pro Trp Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg Thr Val 
                245                 250                 255     


Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys 
            260                 265                 270         


Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg 
        275                 280                 285             


Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn 
    290                 295                 300                 


Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser 
305                 310                 315                 320 


Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys 
                325                 330                 335     


Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr 
            340                 345                 350         


Lys Ser Phe Asn Arg Gly Glu Cys 
        355                 360 


<210>  4
<211>  352
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Made in lab - synthesized WNT agonist

<400>  4

Met Asp Met Arg Val Pro Ala Gln Leu Leu Gly Leu Leu Leu Leu Trp 
1               5                   10                  15      


Leu Arg Gly Ala Arg Cys Gln Val Lys Leu Glu Glu Ser Gly Gly Gly 
            20                  25                  30          


Leu Val Gln Ala Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly 
        35                  40                  45              


Arg Ile Phe Ser Ile Tyr Asp Met Gly Trp Phe Arg Gln Ala Pro Gly 
    50                  55                  60                  


Lys Glu Arg Glu Phe Val Ser Gly Ile Arg Trp Ser Gly Gly Thr Ser 
65                  70                  75                  80  


Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Lys Asp Asn Ala 
                85                  90                  95      


Lys Asn Thr Ile Tyr Leu Gln Met Asn Asn Leu Lys Ala Glu Asp Thr 
            100                 105                 110         


Ala Val Tyr Tyr Cys Gly Ser Arg Gly Tyr Trp Gly Gln Gly Thr Leu 
        115                 120                 125             


Val Thr Val Ser Ser Gly Gly Ser Gly Ser Asp Ile Gln Met Thr Gln 
    130                 135                 140                 


Ser Pro Ser Ser Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr 
145                 150                 155                 160 


Cys Arg Ala Ser Gln Gly Ile Ser Ser Tyr Leu Ala Trp Tyr Gln Gln 
                165                 170                 175     


Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile Tyr Ala Ala Ser Asn Leu 
            180                 185                 190         


Leu Gly Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp 
        195                 200                 205             


Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr 
    210                 215                 220                 


Tyr Cys Gln Gln Thr Tyr Ser Thr Pro Trp Thr Phe Gly Gln Gly Thr 
225                 230                 235                 240 


Lys Val Glu Ile Lys Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe 
                245                 250                 255     


Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys 
            260                 265                 270         


Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val 
        275                 280                 285             


Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln Glu Ser Val Thr Glu Gln 
    290                 295                 300                 


Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser 
305                 310                 315                 320 


Lys Ala Asp Tyr Glu Lys His Lys Val Tyr Ala Cys Glu Val Thr His 
                325                 330                 335     


Gln Gly Leu Ser Ser Pro Val Thr Lys Ser Phe Asn Arg Gly Glu Cys 
            340                 345                 350         


<210>  5
<211>  358
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Made in lab - synthesized WNT agonist

<400>  5

Met Asp Met Arg Val Pro Ala Gln Leu Leu Gly Leu Leu Leu Leu Trp 
1               5                   10                  15      


Leu Arg Gly Ala Arg Cys Asp Val Gln Leu Val Glu Ser Gly Gly Gly 
            20                  25                  30          


Leu Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Thr Ser Ser Ala 
        35                  40                  45              


Asn Ile Asn Ser Ile Glu Thr Leu Gly Trp Tyr Arg Gln Ala Pro Gly 
    50                  55                  60                  


Lys Gln Arg Glu Leu Ile Ala Asn Met Arg Gly Gly Gly Tyr Met Lys 
65                  70                  75                  80  


Tyr Ala Gly Ser Leu Lys Gly Arg Phe Thr Met Ser Thr Glu Ser Ala 
                85                  90                  95      


Lys Asn Thr Met Tyr Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr 
            100                 105                 110         


Ala Val Tyr Tyr Cys Tyr Val Lys Leu Arg Asp Asp Asp Tyr Val Tyr 
        115                 120                 125             


Arg Gly Gln Gly Thr Gln Val Thr Val Ser Ser Gly Gly Ser Gly Ser 
    130                 135                 140                 


Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
145                 150                 155                 160 


Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Ser Ile Ser Ser Tyr 
                165                 170                 175     


Leu Asn Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
            180                 185                 190         


Tyr Ala Ala Ser Ser Leu Gln Ser Gly Val Pro Ser Arg Phe Ser Gly 
        195                 200                 205             


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
    210                 215                 220                 


Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Ser Tyr Ser Thr Pro Leu 
225                 230                 235                 240 


Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys Arg Thr Val Ala Ala 
                245                 250                 255     


Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly 
            260                 265                 270         


Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala 
        275                 280                 285             


Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln 
    290                 295                 300                 


Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser 
305                 310                 315                 320 


Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val Tyr 
                325                 330                 335     


Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys Ser 
            340                 345                 350         


Phe Asn Arg Gly Glu Cys 
        355             


<210>  6
<211>  472
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Made in lab - synthesized WNT agonist

<400>  6

Met Asp Met Arg Val Pro Ala Gln Leu Leu Gly Leu Leu Leu Leu Trp 
1               5                   10                  15      


Leu Arg Gly Ala Arg Cys Glu Val Gln Leu Val Glu Ser Gly Gly Gly 
            20                  25                  30          


Leu Val Lys Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly 
        35                  40                  45              


Phe Thr Phe Thr Asn Tyr Ala Met Ser Trp Val Arg Gln Ala Pro Gly 
    50                  55                  60                  


Lys Gly Leu Glu Trp Val Ser Ala Ile Ser Gly Ser Gly Gly Ser Thr 
65                  70                  75                  80  


Tyr Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asp 
                85                  90                  95      


Ser Lys Asn Thr Leu Tyr Leu Gln Met Asn Ser Leu Lys Thr Glu Asp 
            100                 105                 110         


Thr Ala Val Tyr Tyr Cys Ala Arg Ala Thr Gly Phe Gly Thr Val Val 
        115                 120                 125             


Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Ala Ser 
    130                 135                 140                 


Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr 
145                 150                 155                 160 


Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro 
                165                 170                 175     


Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val 
            180                 185                 190         


His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser 
        195                 200                 205             


Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile 
    210                 215                 220                 


Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys Lys Val 
225                 230                 235                 240 


Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala 
                245                 250                 255     


Pro Glu Ala Ala Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro 
            260                 265                 270         


Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val 
        275                 280                 285             


Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val 
    290                 295                 300                 


Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln 
305                 310                 315                 320 


Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln 
                325                 330                 335     


Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala 
            340                 345                 350         


Leu Gly Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro 
        355                 360                 365             


Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr 
    370                 375                 380                 


Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser 
385                 390                 395                 400 


Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr 
                405                 410                 415     


Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr 
            420                 425                 430         


Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe 
        435                 440                 445             


Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys 
    450                 455                 460                 


Ser Leu Ser Leu Ser Pro Gly Lys 
465                 470         


<210>  7
<211>  360
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Made in lab - synthesized WNT agonist

<400>  7

Met Asp Met Arg Val Pro Ala Gln Leu Leu Gly Leu Leu Leu Leu Trp 
1               5                   10                  15      


Leu Arg Gly Ala Arg Cys Asp Val Gln Leu Val Glu Ser Gly Gly Gly 
            20                  25                  30          


Leu Val Gln Ala Gly Gly Ser Leu Arg Leu Ala Cys Ala Gly Ser Gly 
        35                  40                  45              


Arg Ile Phe Ala Ile Tyr Asp Ile Ala Trp Tyr Arg His Pro Pro Gly 
    50                  55                  60                  


Asn Gln Arg Glu Leu Val Ala Met Ile Arg Pro Val Val Thr Glu Ile 
65                  70                  75                  80  


Asp Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asn Asn 
                85                  90                  95      


Ala Met Lys Thr Val Tyr Leu Gln Met Asn Asn Leu Lys Pro Glu Asp 
            100                 105                 110         


Thr Ala Val Tyr Tyr Cys Asn Ala Lys Arg Pro Trp Gly Ser Arg Asp 
        115                 120                 125             


Glu Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser Gly Gly Ser 
    130                 135                 140                 


Gly Ser Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser 
145                 150                 155                 160 


Val Gly Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Ser Ile Ser 
                165                 170                 175     


Ser Tyr Leu Asn Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu 
            180                 185                 190         


Leu Ile Tyr Ala Ala Ser Ser Leu Gln Ser Gly Val Pro Ser Arg Phe 
        195                 200                 205             


Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu 
    210                 215                 220                 


Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Ser Tyr Ser Thr 
225                 230                 235                 240 


Pro Leu Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys Arg Thr Val 
                245                 250                 255     


Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys 
            260                 265                 270         


Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg 
        275                 280                 285             


Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn 
    290                 295                 300                 


Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser 
305                 310                 315                 320 


Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys 
                325                 330                 335     


Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr 
            340                 345                 350         


Lys Ser Phe Asn Arg Gly Glu Cys 
        355                 360 


<210>  8
<211>  352
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Made in lab - synthesized WNT agonist

<400>  8

Met Asp Met Arg Val Pro Ala Gln Leu Leu Gly Leu Leu Leu Leu Trp 
1               5                   10                  15      


Leu Arg Gly Ala Arg Cys Gln Val Lys Leu Glu Glu Ser Gly Gly Gly 
            20                  25                  30          


Leu Val Gln Ala Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly 
        35                  40                  45              


Arg Ile Phe Ser Ile Tyr Asp Met Gly Trp Phe Arg Gln Ala Pro Gly 
    50                  55                  60                  


Lys Glu Arg Glu Phe Val Ser Gly Ile Arg Trp Ser Gly Gly Thr Ser 
65                  70                  75                  80  


Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Lys Asp Asn Ala 
                85                  90                  95      


Lys Asn Thr Ile Tyr Leu Gln Met Asn Asn Leu Lys Ala Glu Asp Thr 
            100                 105                 110         


Ala Val Tyr Tyr Cys Gly Ser Arg Gly Tyr Trp Gly Gln Gly Thr Leu 
        115                 120                 125             


Val Thr Val Ser Ser Gly Gly Ser Gly Ser Asp Ile Gln Met Thr Gln 
    130                 135                 140                 


Ser Pro Ser Ser Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr 
145                 150                 155                 160 


Cys Arg Ala Ser Gln Ser Ile Ser Ser Tyr Leu Asn Trp Tyr Gln Gln 
                165                 170                 175     


Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile Tyr Ala Ala Ser Ser Leu 
            180                 185                 190         


Gln Ser Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp 
        195                 200                 205             


Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr 
    210                 215                 220                 


Tyr Cys Gln Gln Ser Tyr Ser Thr Pro Leu Thr Phe Gly Gly Gly Thr 
225                 230                 235                 240 


Lys Val Glu Ile Lys Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe 
                245                 250                 255     


Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys 
            260                 265                 270         


Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val 
        275                 280                 285             


Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln Glu Ser Val Thr Glu Gln 
    290                 295                 300                 


Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser 
305                 310                 315                 320 


Lys Ala Asp Tyr Glu Lys His Lys Val Tyr Ala Cys Glu Val Thr His 
                325                 330                 335     


Gln Gly Leu Ser Ser Pro Val Thr Lys Ser Phe Asn Arg Gly Glu Cys 
            340                 345                 350         


