                         SEQUENCE LISTING

<110>  Northwestern University
       Jewett, Michael Christopher
       Kightlinger, Weston
 
<120>  MODULAR PLATFORM FOR PRODUCING GLYCOPROTEINS AND IDENTIFYING 
       GLYCOSYLATION PATHWAYS

<130>  5369-00600

<160>  20    

<170>  PatentIn version 3.5

<210>  1
<211>  620
<212>  PRT
<213>  Actinobacillus pleuropneumoniae

<400>  1

Met Glu Asn Glu Asn Lys Pro Asn Val Ala Asn Phe Glu Ala Ala Val 
1               5                   10                  15      


Ala Ala Lys Asp Tyr Glu Lys Ala Cys Ser Glu Leu Leu Leu Ile Leu 
            20                  25                  30          


Ser Gln Leu Asp Ser Asn Phe Gly Gly Ile His Glu Ile Glu Phe Glu 
        35                  40                  45              


Tyr Pro Ala Gln Leu Gln Asp Leu Glu Gln Glu Lys Ile Val Tyr Phe 
    50                  55                  60                  


Cys Thr Arg Met Ala Thr Ala Ile Thr Thr Leu Phe Ser Asp Pro Val 
65                  70                  75                  80  


Leu Glu Ile Ser Asp Leu Gly Val Gln Arg Phe Leu Val Tyr Gln Arg 
                85                  90                  95      


Trp Leu Ala Leu Ile Phe Ala Ser Ser Pro Phe Val Asn Ala Asp His 
            100                 105                 110         


Ile Leu Gln Thr Tyr Asn Arg Glu Pro Asn Arg Lys Asn Ser Leu Glu 
        115                 120                 125             


Ile His Leu Asp Ser Ser Lys Ser Ser Leu Ile Lys Phe Cys Ile Leu 
    130                 135                 140                 


Tyr Leu Pro Glu Ser Asn Val Asn Leu Asn Leu Asp Val Met Trp Asn 
145                 150                 155                 160 


Ile Ser Pro Glu Leu Cys Ala Ser Leu Cys Phe Ala Leu Gln Ser Pro 
                165                 170                 175     


Arg Phe Val Gly Thr Ser Thr Ala Phe Asn Lys Arg Ala Thr Ile Leu 
            180                 185                 190         


Gln Trp Phe Pro Arg His Leu Asp Gln Leu Lys Asn Leu Asn Asn Ile 
        195                 200                 205             


Pro Ser Ala Ile Ser His Asp Val Tyr Met His Cys Ser Tyr Asp Thr 
    210                 215                 220                 


Ser Val Asn Lys His Asp Val Lys Arg Ala Leu Asn His Val Ile Arg 
225                 230                 235                 240 


Arg His Ile Glu Ser Glu Tyr Gly Trp Lys Asp Arg Asp Val Ala His 
                245                 250                 255     


Ile Gly Tyr Arg Asn Asn Lys Pro Val Met Val Val Leu Leu Glu His 
            260                 265                 270         


Phe His Ser Ala His Ser Ile Tyr Arg Thr His Ser Thr Ser Met Ile 
        275                 280                 285             


Ala Ala Arg Glu His Phe Tyr Leu Ile Gly Leu Gly Ser Pro Ser Val 
    290                 295                 300                 


Asp Gln Ala Gly Gln Glu Val Phe Asp Glu Phe His Leu Val Ala Gly 
305                 310                 315                 320 


Asp Asn Met Lys Gln Lys Leu Glu Phe Ile Arg Ser Val Cys Glu Ser 
                325                 330                 335     


Asn Gly Ala Ala Ile Phe Tyr Met Pro Ser Ile Gly Met Asp Met Thr 
            340                 345                 350         


Thr Ile Phe Ala Ser Asn Thr Arg Leu Ala Pro Ile Gln Ala Ile Ala 
        355                 360                 365             


Leu Gly His Pro Ala Thr Thr His Ser Asp Phe Ile Glu Tyr Val Ile 
    370                 375                 380                 


Val Glu Asp Asp Tyr Val Gly Ser Glu Glu Cys Phe Ser Glu Thr Leu 
385                 390                 395                 400 


Leu Arg Leu Pro Lys Asp Ala Leu Pro Tyr Val Pro Ser Ala Leu Ala 
                405                 410                 415     


Pro Glu Lys Val Asp Tyr Leu Leu Arg Glu Asn Pro Glu Val Val Asn 
            420                 425                 430         


Ile Gly Ile Ala Ser Thr Thr Met Lys Leu Asn Pro Tyr Phe Leu Glu 
        435                 440                 445             


Ala Leu Lys Ala Ile Arg Asp Arg Ala Lys Val Lys Val His Phe His 
    450                 455                 460                 


Phe Ala Leu Gly Gln Ser Asn Gly Ile Thr His Pro Tyr Val Glu Arg 
465                 470                 475                 480 


Phe Ile Lys Ser Tyr Leu Gly Asp Ser Ala Thr Ala His Pro His Ser 
                485                 490                 495     


Pro Tyr His Gln Tyr Leu Arg Ile Leu His Asn Cys Asp Met Met Val 
            500                 505                 510         


Asn Pro Phe Pro Phe Gly Asn Thr Asn Gly Ile Ile Asp Met Val Thr 
        515                 520                 525             


Leu Gly Leu Val Gly Val Cys Lys Thr Gly Ala Glu Val His Glu His 
    530                 535                 540                 


Ile Asp Glu Gly Leu Phe Lys Arg Leu Gly Leu Pro Glu Trp Leu Ile 
545                 550                 555                 560 


Ala Asn Thr Val Asp Glu Tyr Val Glu Arg Ala Val Arg Leu Ala Glu 
                565                 570                 575     


Asn His Gln Glu Arg Leu Glu Leu Arg Arg Tyr Ile Ile Glu Asn Asn 
            580                 585                 590         


Gly Leu Asn Thr Leu Phe Thr Gly Asp Pro Arg Pro Met Gly Gln Val 
        595                 600                 605             


Phe Leu Glu Lys Leu Asn Ala Phe Leu Lys Glu Asn 
    610                 615                 620 


<210>  2
<211>  620
<212>  PRT
<213>  Artificial

<220>
<223>  Modified Actinobacillus pleuropneumoniae NGT

<400>  2

Met Glu Asn Glu Asn Lys Pro Asn Val Ala Asn Phe Glu Ala Ala Val 
1               5                   10                  15      


Ala Ala Lys Asp Tyr Glu Lys Ala Cys Ser Glu Leu Leu Leu Ile Leu 
            20                  25                  30          


Ser Gln Leu Asp Ser Asn Phe Gly Gly Ile His Glu Ile Glu Phe Glu 
        35                  40                  45              


Tyr Pro Ala Gln Leu Gln Asp Leu Glu Gln Glu Lys Ile Val Tyr Phe 
    50                  55                  60                  


Cys Thr Arg Met Ala Thr Ala Ile Thr Thr Leu Phe Ser Asp Pro Val 
65                  70                  75                  80  


Leu Glu Ile Ser Asp Leu Gly Val Gln Arg Phe Leu Val Tyr Gln Arg 
                85                  90                  95      


Trp Leu Ala Leu Ile Phe Ala Ser Ser Pro Phe Val Asn Ala Asp His 
            100                 105                 110         


Ile Leu Gln Thr Tyr Asn Arg Glu Pro Asn Arg Lys Asn Ser Leu Glu 
        115                 120                 125             


Ile His Leu Asp Ser Ser Lys Ser Ser Leu Ile Lys Phe Cys Ile Leu 
    130                 135                 140                 


Tyr Leu Pro Glu Ser Asn Val Asn Leu Asn Leu Asp Val Met Trp Asn 
145                 150                 155                 160 


Ile Ser Pro Glu Leu Cys Ala Ser Leu Cys Phe Ala Leu Gln Ser Pro 
                165                 170                 175     


Arg Phe Val Gly Thr Ser Thr Ala Phe Asn Lys Arg Ala Thr Ile Leu 
            180                 185                 190         


Gln Trp Phe Pro Arg His Leu Asp Gln Leu Lys Asn Leu Asn Asn Ile 
        195                 200                 205             


Pro Ser Ala Ile Ser His Asp Val Tyr Met His Cys Ser Tyr Asp Thr 
    210                 215                 220                 


Ser Val Asn Lys His Asp Val Lys Arg Ala Leu Asn His Val Ile Arg 
225                 230                 235                 240 


Arg His Ile Glu Ser Glu Tyr Gly Trp Lys Asp Arg Asp Val Ala His 
                245                 250                 255     


Ile Gly Tyr Arg Asn Asn Lys Pro Val Met Val Val Leu Leu Glu His 
            260                 265                 270         


Phe His Ser Ala His Ser Ile Tyr Arg Thr His Ser Thr Ser Met Ile 
        275                 280                 285             


Ala Ala Arg Glu His Phe Tyr Leu Ile Gly Leu Gly Ser Pro Ser Val 
    290                 295                 300                 


Asp Gln Ala Gly Gln Glu Val Phe Asp Glu Phe His Leu Val Ala Gly 
305                 310                 315                 320 


Asp Asn Met Lys Gln Lys Leu Glu Phe Ile Arg Ser Val Cys Glu Ser 
                325                 330                 335     


Asn Gly Ala Ala Ile Phe Tyr Met Pro Ser Ile Gly Met Asp Met Thr 
            340                 345                 350         


Thr Ile Phe Ala Ser Asn Thr Arg Leu Ala Pro Ile Gln Ala Ile Ala 
        355                 360                 365             


Leu Gly His Pro Ala Thr Thr His Ser Asp Phe Ile Glu Tyr Val Ile 
    370                 375                 380                 


Val Glu Asp Asp Tyr Val Gly Ser Glu Glu Cys Phe Ser Glu Thr Leu 
385                 390                 395                 400 


Leu Arg Leu Pro Lys Asp Ala Leu Pro Tyr Val Pro Ser Ala Leu Ala 
                405                 410                 415     


Pro Glu Lys Val Asp Tyr Leu Leu Arg Glu Asn Pro Glu Val Val Asn 
            420                 425                 430         


Ile Gly Ile Ala Ser Thr Thr Met Lys Leu Asn Pro Tyr Phe Leu Glu 
        435                 440                 445             


Ala Leu Lys Ala Ile Arg Asp Arg Ala Lys Val Lys Val His Phe His 
    450                 455                 460                 


Phe Ala Leu Gly Ala Ser Asn Gly Ile Thr His Pro Tyr Val Glu Arg 
465                 470                 475                 480 


Phe Ile Lys Ser Tyr Leu Gly Asp Ser Ala Thr Ala His Pro His Ser 
                485                 490                 495     


Pro Tyr His Gln Tyr Leu Arg Ile Leu His Asn Cys Asp Met Met Val 
            500                 505                 510         


Asn Pro Phe Pro Phe Gly Asn Thr Asn Gly Ile Ile Asp Met Val Thr 
        515                 520                 525             


Leu Gly Leu Val Gly Val Cys Lys Thr Gly Ala Glu Val His Glu His 
    530                 535                 540                 


Ile Asp Glu Gly Leu Phe Lys Arg Leu Gly Leu Pro Glu Trp Leu Ile 
545                 550                 555                 560 


Ala Asn Thr Val Asp Glu Tyr Val Glu Arg Ala Val Arg Leu Ala Glu 
                565                 570                 575     


Asn His Gln Glu Arg Leu Glu Leu Arg Arg Tyr Ile Ile Glu Asn Asn 
            580                 585                 590         


Gly Leu Asn Thr Leu Phe Thr Gly Asp Pro Arg Pro Met Gly Gln Val 
        595                 600                 605             


Phe Leu Glu Lys Leu Asn Ala Phe Leu Lys Glu Asn 
    610                 615                 620 


<210>  3
<211>  637
<212>  PRT
<213>  Escherichia coli

<400>  3

Met Met Ser His Lys Thr Asp Thr Ala Pro Val Gln Glu Gln Ala Gly 
1               5                   10                  15      


Leu Thr Phe Arg Leu Glu Thr Phe Glu Trp Gln Val His Gln Gly Leu 
            20                  25                  30          


Asn Glu Glu Ala Ala Arg Ser Leu Ile Ser Leu Leu Gln Leu Leu Asp 
        35                  40                  45              


Arg His Tyr Ala Gln Trp Gly Glu Ser Phe Ser Ala Trp Ala Pro Gly 
    50                  55                  60                  


Met Thr Ala Glu Glu Ile Asn Pro His Leu Cys Thr Arg Ile Ala Gly 
65                  70                  75                  80  


Ala Ile Thr Ala Leu Phe Ser Arg Pro Gly Phe Arg Val Ser Asp Gly 
                85                  90                  95      


Gly Phe Ala Glu Leu Met Asp Tyr His Arg Trp Leu Ala Ile Ile Phe 
            100                 105                 110         


Ala Val Ser Asp Tyr Arg His Gly Asp His Ile Ile Arg Asn Ile Asn 
        115                 120                 125             


Ala Ala Gly Gly Gly Val Val Ala Pro Leu Thr Leu Asn Ala Asp Asn 
    130                 135                 140                 


Leu Gln Leu Phe Cys Leu Ser Tyr Tyr Pro Asp Ser Gln Ile Ala Leu 
145                 150                 155                 160 


Gln Pro Glu Pro Leu Trp Gln Tyr Asp Arg Gln Thr Val Val Arg Leu 
                165                 170                 175     


Phe Phe Ala Leu Leu Ser Gly Arg Ala Leu Pro Thr Pro Ala Ala His 
            180                 185                 190         


Gln Lys Arg Glu His Leu Leu Ala Trp Leu Pro Glu Arg Leu Lys Glu 
        195                 200                 205             


Ile Asp Ser Leu Glu Phe Leu Pro Gly Lys Val Leu His Asp Val Tyr 
    210                 215                 220                 


Met His Cys Ser Tyr Ala Asp Leu Pro Glu Lys His Arg Ile Lys Gln 
225                 230                 235                 240 


Glu Ile Asn Arg Leu Thr Ala Arg Ala Leu Glu Gln Thr Tyr Ala Asp 
                245                 250                 255     


Cys Leu Pro Val Arg Ala Pro Glu Ala Ala Arg Gln Lys Pro Val Leu 
            260                 265                 270         


Ala Val Val Leu Glu Trp Phe Thr Cys Gln His Ser Ile Tyr Arg Thr 
        275                 280                 285             


His Ser Thr Ser Met Arg Ala Leu Arg Glu His Phe His Leu Leu Gly 
    290                 295                 300                 


Ile Ala Gln Pro Gly Ala Thr Asp Glu Ile Thr Arg Glu Val Phe Asp 
305                 310                 315                 320 


Glu Phe Arg Glu Leu Ser Ala Glu Asn Val Val Gly Asp Ala Ile Arg 
                325                 330                 335     


Cys Leu Ser Glu Val Arg Pro Asp Val Ile Tyr Tyr Pro Ser Val Gly 
            340                 345                 350         


Met Phe Pro Leu Thr Val Tyr Leu Thr Ala Leu Arg Leu Ala Pro Leu 
        355                 360                 365             


Gln Leu Met Ala Leu Gly His Pro Ala Thr Thr Trp Ser Glu His Ile 
    370                 375                 380                 


Asp Gly Val Leu Val Glu Glu Asp Tyr Leu Gly Asp Pro Ala Cys Phe 
385                 390                 395                 400 


Ser Glu Thr Val Cys Ala Val Pro Lys Asp Ala Ile Pro Tyr Ile Pro 
                405                 410                 415     


Pro Ala Ser Thr Glu Arg Val Leu Pro Glu Arg Thr Pro Phe Arg Asp 
            420                 425                 430         


Arg Ala Lys Ala Ala Trp Pro Ala Ala Leu Pro Val Arg Val Ala Val 
        435                 440                 445             


Cys Ala Ser Val Met Lys Ile Asn Pro Gly Phe Leu Asp Thr Leu Arg 
    450                 455                 460                 


Glu Ile Ser Asp Arg Ser Arg Val Pro Val Gln Phe Cys Phe Trp Met 
465                 470                 475                 480 


Gly Phe Ala Gln Gly Leu Thr Leu Asp Tyr Leu Arg Arg Ala Ile Arg 
                485                 490                 495     


Gln Ala Leu Pro Thr Ala Glu Val Asn Ala His Met Pro Val Gln Ala 
            500                 505                 510         


Tyr Gln Gln Ala Leu Asn Ser Cys Glu Leu Phe Val Asn Pro Phe Pro 
        515                 520                 525             


Phe Gly Asn Thr Asn Gly Leu Val Asp Thr Val Arg Gln Gly Leu Pro 
    530                 535                 540                 


Gly Val Cys Met Thr Gly Pro Glu Val His Thr His Ile Asp Glu Gly 
545                 550                 555                 560 


Leu Phe Arg Arg Leu Gly Leu Pro Glu Ala Leu Ile Ala Arg Asp Arg 
                565                 570                 575     


Glu Glu Tyr Ile Thr Ala Val Leu Ser Leu Thr Glu Thr Pro Arg Leu 
            580                 585                 590         


Arg Glu Arg Leu Gln Lys Tyr Leu Thr Glu Asn Asp Val Glu Lys Val 
        595                 600                 605             


Leu Phe Glu Gly Arg Pro Asp Lys Phe Ala Glu Arg Val Trp Gln Leu 
    610                 615                 620                 


Trp Glu Ala Arg Ser His Arg Gln Glu Glu Gly Ala Glu 
625                 630                 635         


<210>  4
<211>  637
<212>  PRT
<213>  Artificial

<220>
<223>  Modified Escherichia coli NGT

<400>  4

Met Met Ser His Lys Thr Asp Thr Ala Pro Val Gln Glu Gln Ala Gly 
1               5                   10                  15      


Leu Thr Phe Arg Leu Glu Thr Phe Glu Trp Gln Val His Gln Gly Leu 
            20                  25                  30          


Asn Glu Glu Ala Ala Arg Ser Leu Ile Ser Leu Leu Gln Leu Leu Asp 
        35                  40                  45              


Arg His Tyr Ala Gln Trp Gly Glu Ser Phe Ser Ala Trp Ala Pro Gly 
    50                  55                  60                  


Met Thr Ala Glu Glu Ile Asn Pro His Leu Cys Thr Arg Ile Ala Gly 
65                  70                  75                  80  


Ala Ile Thr Ala Leu Phe Ser Arg Pro Gly Phe Arg Val Ser Asp Gly 
                85                  90                  95      


Gly Phe Ala Glu Leu Met Asp Tyr His Arg Trp Leu Ala Ile Ile Phe 
            100                 105                 110         


Ala Val Ser Asp Tyr Arg His Gly Asp His Ile Ile Arg Asn Ile Asn 
        115                 120                 125             


Ala Ala Gly Gly Gly Val Val Ala Pro Leu Thr Leu Asn Ala Asp Asn 
    130                 135                 140                 


Leu Gln Leu Phe Cys Leu Ser Tyr Tyr Pro Asp Ser Gln Ile Ala Leu 
145                 150                 155                 160 


Gln Pro Glu Pro Leu Trp Gln Tyr Asp Arg Gln Thr Val Val Arg Leu 
                165                 170                 175     


Phe Phe Ala Leu Leu Ser Gly Arg Ala Leu Pro Thr Pro Ala Ala His 
            180                 185                 190         


Gln Lys Arg Glu His Leu Leu Ala Trp Leu Pro Glu Arg Leu Lys Glu 
        195                 200                 205             


Ile Asp Ser Leu Glu Phe Leu Pro Gly Lys Val Leu His Asp Val Tyr 
    210                 215                 220                 


Met His Cys Ser Tyr Ala Asp Leu Pro Glu Lys His Arg Ile Lys Gln 
225                 230                 235                 240 


Glu Ile Asn Arg Leu Thr Ala Arg Ala Leu Glu Gln Thr Tyr Ala Asp 
                245                 250                 255     


Cys Leu Pro Val Arg Ala Pro Glu Ala Ala Arg Gln Lys Pro Val Leu 
            260                 265                 270         


Ala Val Val Leu Glu Trp Phe Thr Cys Gln His Ser Ile Tyr Arg Thr 
        275                 280                 285             


His Ser Thr Ser Met Arg Ala Leu Arg Glu His Phe His Leu Leu Gly 
    290                 295                 300                 


Ile Ala Gln Pro Gly Ala Thr Asp Glu Ile Thr Arg Glu Val Phe Asp 
305                 310                 315                 320 


Glu Phe Arg Glu Leu Ser Ala Glu Asn Val Val Gly Asp Ala Ile Arg 
                325                 330                 335     


Cys Leu Ser Glu Val Arg Pro Asp Val Ile Tyr Tyr Pro Ser Val Gly 
            340                 345                 350         


Met Phe Pro Leu Thr Val Tyr Leu Thr Ala Leu Arg Leu Ala Pro Leu 
        355                 360                 365             


Gln Leu Met Ala Leu Gly His Pro Ala Thr Thr Trp Ser Glu His Ile 
    370                 375                 380                 


Asp Gly Val Leu Val Glu Glu Asp Tyr Leu Gly Asp Pro Ala Cys Phe 
385                 390                 395                 400 


Ser Glu Thr Val Cys Ala Val Pro Lys Asp Ala Ile Pro Tyr Ile Pro 
                405                 410                 415     


Pro Ala Ser Thr Glu Arg Val Leu Pro Glu Arg Thr Pro Phe Arg Asp 
            420                 425                 430         


Arg Ala Lys Ala Ala Trp Pro Ala Ala Leu Pro Val Arg Val Ala Val 
        435                 440                 445             


Cys Ala Ser Val Met Lys Ile Asn Pro Gly Phe Leu Asp Thr Leu Arg 
    450                 455                 460                 


Glu Ile Ser Asp Arg Ser Arg Val Pro Val Gln Phe Cys Phe Trp Met 
465                 470                 475                 480 


Gly Ala Ala Gln Gly Leu Thr Leu Asp Tyr Leu Arg Arg Ala Ile Arg 
                485                 490                 495     


Gln Ala Leu Pro Thr Ala Glu Val Asn Ala His Met Pro Val Gln Ala 
            500                 505                 510         


Tyr Gln Gln Ala Leu Asn Ser Cys Glu Leu Phe Val Asn Pro Phe Pro 
        515                 520                 525             


Phe Gly Asn Thr Asn Gly Leu Val Asp Thr Val Arg Gln Gly Leu Pro 
    530                 535                 540                 


Gly Val Cys Met Thr Gly Pro Glu Val His Thr His Ile Asp Glu Gly 
545                 550                 555                 560 


Leu Phe Arg Arg Leu Gly Leu Pro Glu Ala Leu Ile Ala Arg Asp Arg 
                565                 570                 575     


Glu Glu Tyr Ile Thr Ala Val Leu Ser Leu Thr Glu Thr Pro Arg Leu 
            580                 585                 590         


Arg Glu Arg Leu Gln Lys Tyr Leu Thr Glu Asn Asp Val Glu Lys Val 
        595                 600                 605             


Leu Phe Glu Gly Arg Pro Asp Lys Phe Ala Glu Arg Val Trp Gln Leu 
    610                 615                 620                 


Trp Glu Ala Arg Ser His Arg Gln Glu Glu Gly Ala Glu 
625                 630                 635         


<210>  5
<211>  650
<212>  PRT
<213>  Haemophilus influenza

<400>  5

Met Thr Lys Glu Asn Leu Gln Ser Val Pro Gln Asn Thr Thr Ala Ser 
1               5                   10                  15      


Leu Val Glu Ser Asn Asn Asp Gln Thr Ser Leu Gln Ile Leu Lys Gln 
            20                  25                  30          


Pro Pro Lys Pro Asn Leu Leu Arg Leu Glu Gln His Val Ala Lys Lys 
        35                  40                  45              


Asp Tyr Glu Leu Ala Cys Arg Glu Leu Met Ala Ile Leu Glu Lys Met 
    50                  55                  60                  


Asp Ala Asn Phe Gly Gly Val His Asp Ile Glu Phe Asp Ala Pro Ala 
65                  70                  75                  80  


Gln Leu Ala Tyr Leu Pro Glu Lys Leu Leu Ile His Phe Ala Thr Arg 
                85                  90                  95      


Leu Ala Asn Ala Ile Thr Thr Leu Phe Ser Asp Pro Glu Leu Ala Ile 
            100                 105                 110         


Ser Glu Glu Gly Ala Leu Lys Met Ile Ser Leu Gln Arg Trp Leu Thr 
        115                 120                 125             


Leu Ile Phe Ala Ser Ser Pro Tyr Val Asn Ala Asp His Ile Leu Asn 
    130                 135                 140                 


Lys Tyr Asn Ile Asn Pro Asp Ser Glu Gly Gly Phe His Leu Ala Thr 
145                 150                 155                 160 


Asp Asn Ser Ser Ile Ala Lys Phe Cys Ile Phe Tyr Leu Pro Glu Ser 
                165                 170                 175     


Asn Val Asn Met Ser Leu Asp Ala Leu Trp Ala Gly Asn Gln Gln Leu 
            180                 185                 190         


Cys Ala Ser Leu Cys Phe Ala Leu Gln Ser Ser Arg Phe Ile Gly Thr 
        195                 200                 205             


Ala Ser Ala Phe His Lys Arg Ala Val Val Leu Gln Trp Phe Pro Lys 
    210                 215                 220                 


Lys Leu Ala Glu Ile Ala Asn Leu Asp Glu Leu Pro Ala Asn Ile Leu 
225                 230                 235                 240 


His Asp Val Tyr Met His Cys Ser Tyr Asp Leu Ala Lys Asn Lys His 
                245                 250                 255     


Asp Val Lys Arg Pro Leu Asn Glu Leu Val Arg Lys His Ile Leu Thr 
            260                 265                 270         


Gln Gly Trp Gln Asp Arg Tyr Leu Tyr Thr Leu Gly Lys Lys Asp Gly 
        275                 280                 285             


Lys Pro Val Met Met Val Leu Leu Glu His Phe Asn Ser Gly His Ser 
    290                 295                 300                 


Ile Tyr Arg Thr His Ser Thr Ser Met Ile Ala Ala Arg Glu Lys Phe 
305                 310                 315                 320 


Tyr Leu Val Gly Leu Gly His Glu Gly Val Asp Asn Ile Gly Arg Glu 
                325                 330                 335     


Val Phe Asp Glu Phe Phe Glu Ile Ser Ser Asn Asn Ile Met Glu Arg 
            340                 345                 350         


Leu Phe Phe Ile Arg Lys Gln Cys Glu Thr Phe Gln Pro Ala Val Phe 
        355                 360                 365             


Tyr Met Pro Ser Ile Gly Met Asp Ile Thr Thr Ile Phe Val Ser Asn 
    370                 375                 380                 


Thr Arg Leu Ala Pro Ile Gln Ala Val Ala Leu Gly His Pro Ala Thr 
385                 390                 395                 400 


Thr His Ser Glu Phe Ile Asp Tyr Val Ile Val Glu Asp Asp Tyr Val 
                405                 410                 415     


Gly Ser Glu Asp Cys Phe Ser Glu Thr Leu Leu Arg Leu Pro Lys Asp 
            420                 425                 430         


Ala Leu Pro Tyr Val Pro Ser Ala Leu Ala Pro Gln Lys Val Asp Tyr 
        435                 440                 445             


Val Leu Arg Glu Asn Pro Glu Val Val Asn Ile Gly Ile Ala Ala Thr 
    450                 455                 460                 


Thr Met Lys Leu Asn Pro Glu Phe Leu Leu Thr Leu Gln Glu Ile Arg 
465                 470                 475                 480 


Asp Lys Ala Lys Val Lys Ile His Phe His Phe Ala Leu Gly Gln Ser 
                485                 490                 495     


Thr Gly Leu Thr His Pro Tyr Val Lys Trp Phe Ile Glu Ser Tyr Leu 
            500                 505                 510         


Gly Asp Asp Ala Thr Ala His Pro His Ala Pro Tyr His Asp Tyr Leu 
        515                 520                 525             


Ala Ile Leu Arg Asp Cys Asp Met Leu Leu Asn Pro Phe Pro Phe Gly 
    530                 535                 540                 


Asn Thr Asn Gly Ile Ile Asp Met Val Thr Leu Gly Leu Val Gly Val 
545                 550                 555                 560 


Cys Lys Thr Gly Asp Glu Val His Glu His Ile Asp Glu Gly Leu Phe 
                565                 570                 575     


Lys Arg Leu Gly Leu Pro Glu Trp Leu Ile Ala Asp Thr Arg Glu Thr 
            580                 585                 590         


Tyr Ile Glu Cys Ala Leu Arg Leu Ala Glu Asn His Gln Glu Arg Leu 
        595                 600                 605             


Glu Leu Arg Arg Tyr Ile Ile Glu Asn Asn Gly Leu Gln Lys Leu Phe 
    610                 615                 620                 


Thr Gly Asp Pro Arg Pro Leu Gly Lys Ile Leu Leu Lys Lys Thr Asn 
625                 630                 635                 640 


Glu Trp Lys Arg Lys His Leu Ser Lys Lys 
                645                 650 


<210>  6
<211>  650
<212>  PRT
<213>  Artificial

<220>
<223>  Modified Haemophilus influenza NGT

<400>  6

Met Thr Lys Glu Asn Leu Gln Ser Val Pro Gln Asn Thr Thr Ala Ser 
1               5                   10                  15      


Leu Val Glu Ser Asn Asn Asp Gln Thr Ser Leu Gln Ile Leu Lys Gln 
            20                  25                  30          


Pro Pro Lys Pro Asn Leu Leu Arg Leu Glu Gln His Val Ala Lys Lys 
        35                  40                  45              


Asp Tyr Glu Leu Ala Cys Arg Glu Leu Met Ala Ile Leu Glu Lys Met 
    50                  55                  60                  


Asp Ala Asn Phe Gly Gly Val His Asp Ile Glu Phe Asp Ala Pro Ala 
65                  70                  75                  80  


Gln Leu Ala Tyr Leu Pro Glu Lys Leu Leu Ile His Phe Ala Thr Arg 
                85                  90                  95      


Leu Ala Asn Ala Ile Thr Thr Leu Phe Ser Asp Pro Glu Leu Ala Ile 
            100                 105                 110         


Ser Glu Glu Gly Ala Leu Lys Met Ile Ser Leu Gln Arg Trp Leu Thr 
        115                 120                 125             


Leu Ile Phe Ala Ser Ser Pro Tyr Val Asn Ala Asp His Ile Leu Asn 
    130                 135                 140                 


Lys Tyr Asn Ile Asn Pro Asp Ser Glu Gly Gly Phe His Leu Ala Thr 
145                 150                 155                 160 


Asp Asn Ser Ser Ile Ala Lys Phe Cys Ile Phe Tyr Leu Pro Glu Ser 
                165                 170                 175     


Asn Val Asn Met Ser Leu Asp Ala Leu Trp Ala Gly Asn Gln Gln Leu 
            180                 185                 190         


Cys Ala Ser Leu Cys Phe Ala Leu Gln Ser Ser Arg Phe Ile Gly Thr 
        195                 200                 205             


Ala Ser Ala Phe His Lys Arg Ala Val Val Leu Gln Trp Phe Pro Lys 
    210                 215                 220                 


Lys Leu Ala Glu Ile Ala Asn Leu Asp Glu Leu Pro Ala Asn Ile Leu 
225                 230                 235                 240 


His Asp Val Tyr Met His Cys Ser Tyr Asp Leu Ala Lys Asn Lys His 
                245                 250                 255     


Asp Val Lys Arg Pro Leu Asn Glu Leu Val Arg Lys His Ile Leu Thr 
            260                 265                 270         


Gln Gly Trp Gln Asp Arg Tyr Leu Tyr Thr Leu Gly Lys Lys Asp Gly 
        275                 280                 285             


Lys Pro Val Met Met Val Leu Leu Glu His Phe Asn Ser Gly His Ser 
    290                 295                 300                 


Ile Tyr Arg Thr His Ser Thr Ser Met Ile Ala Ala Arg Glu Lys Phe 
305                 310                 315                 320 


Tyr Leu Val Gly Leu Gly His Glu Gly Val Asp Asn Ile Gly Arg Glu 
                325                 330                 335     


Val Phe Asp Glu Phe Phe Glu Ile Ser Ser Asn Asn Ile Met Glu Arg 
            340                 345                 350         


Leu Phe Phe Ile Arg Lys Gln Cys Glu Thr Phe Gln Pro Ala Val Phe 
        355                 360                 365             


Tyr Met Pro Ser Ile Gly Met Asp Ile Thr Thr Ile Phe Val Ser Asn 
    370                 375                 380                 


Thr Arg Leu Ala Pro Ile Gln Ala Val Ala Leu Gly His Pro Ala Thr 
385                 390                 395                 400 


Thr His Ser Glu Phe Ile Asp Tyr Val Ile Val Glu Asp Asp Tyr Val 
                405                 410                 415     


Gly Ser Glu Asp Cys Phe Ser Glu Thr Leu Leu Arg Leu Pro Lys Asp 
            420                 425                 430         


Ala Leu Pro Tyr Val Pro Ser Ala Leu Ala Pro Gln Lys Val Asp Tyr 
        435                 440                 445             


Val Leu Arg Glu Asn Pro Glu Val Val Asn Ile Gly Ile Ala Ala Thr 
    450                 455                 460                 


Thr Met Lys Leu Asn Pro Glu Phe Leu Leu Thr Leu Gln Glu Ile Arg 
465                 470                 475                 480 


Asp Lys Ala Lys Val Lys Ile His Phe His Phe Ala Leu Gly Ala Ser 
                485                 490                 495     


Thr Gly Leu Thr His Pro Tyr Val Lys Trp Phe Ile Glu Ser Tyr Leu 
            500                 505                 510         


Gly Asp Asp Ala Thr Ala His Pro His Ala Pro Tyr His Asp Tyr Leu 
        515                 520                 525             


Ala Ile Leu Arg Asp Cys Asp Met Leu Leu Asn Pro Phe Pro Phe Gly 
    530                 535                 540                 


Asn Thr Asn Gly Ile Ile Asp Met Val Thr Leu Gly Leu Val Gly Val 
545                 550                 555                 560 


Cys Lys Thr Gly Asp Glu Val His Glu His Ile Asp Glu Gly Leu Phe 
                565                 570                 575     


Lys Arg Leu Gly Leu Pro Glu Trp Leu Ile Ala Asp Thr Arg Glu Thr 
            580                 585                 590         


Tyr Ile Glu Cys Ala Leu Arg Leu Ala Glu Asn His Gln Glu Arg Leu 
        595                 600                 605             


Glu Leu Arg Arg Tyr Ile Ile Glu Asn Asn Gly Leu Gln Lys Leu Phe 
    610                 615                 620                 


Thr Gly Asp Pro Arg Pro Leu Gly Lys Ile Leu Leu Lys Lys Thr Asn 
625                 630                 635                 640 


Glu Trp Lys Arg Lys His Leu Ser Lys Lys 
                645                 650 


<210>  7
<211>  669
<212>  PRT
<213>  Mannheimia haemolytica

<400>  7

Met Ser Ala Glu Asn Met Pro Ser Val Ile Arg Phe Glu Gln Ala Val 
1               5                   10                  15      


Ala Lys Lys Asp Tyr Glu Ser Ala Cys Thr Glu Leu Leu Ser Ile Leu 
            20                  25                  30          


Ser Lys Leu Asp Ser Asn Phe Gly Gly Ile Ser Asn Ile Glu Leu Asn 
        35                  40                  45              


Met Pro Glu Gln Ile Glu Asn Leu Glu Asn Asp Lys Ala Ile Tyr Phe 
    50                  55                  60                  


Cys Thr Arg Met Ala Val Ala Ile Thr Arg Leu Phe Glu Asp Pro Ala 
65                  70                  75                  80  


Leu Glu Ile Ser Glu His Gly Ala Met Arg Phe Leu Thr Leu Gln Arg 
                85                  90                  95      


Trp Ile Ala Leu Ile Phe Ala Ser Ser Pro Tyr Val Asn Ala Asp His 
            100                 105                 110         


Ile Leu Arg Thr Tyr Asn Arg Asn Lys Glu Ser Ala Asn Pro Asn Thr 
        115                 120                 125             


Val Asp Leu Asp Ala Thr Leu Gln Ala Leu Ile Lys Phe Cys Ile Leu 
    130                 135                 140                 


Tyr Leu Pro Glu Ser Asn Ile Leu Leu Asn Leu Asp Ala Ala Trp Asn 
145                 150                 155                 160 


Ala Ser Ser Asp Leu Thr Ala Ser Leu Cys Phe Ala Leu Gln Ser Pro 
                165                 170                 175     


Arg Phe Ile Gly Thr Ser Ser Ala Phe Ala Lys Arg Ala Ala Ile Leu 
            180                 185                 190         


Gln Trp Phe Pro Glu Lys Leu Ala Gln Ile Glu Asn Leu Asn Lys Leu 
        195                 200                 205             


Pro Ser Ala Ile Ser His Asp Val Tyr Met His Cys Ser Tyr Asp Ile 
    210                 215                 220                 


Glu Ala Asn Lys His Asn Val Lys Arg Ser Leu Asn Ala Val Ile Arg 
225                 230                 235                 240 


Arg His Leu Leu Ser Val Gly Trp Glu Asp Arg Lys Ile Glu Gln Leu 
                245                 250                 255     


Gly Thr Arg Asn Asn Lys Pro Val Met Val Val Leu Leu Glu His Phe 
            260                 265                 270         


His Ser Ser His Ser Ile Tyr Arg Thr His Ser Thr Ser Met Val Ala 
        275                 280                 285             


Ala Arg Glu His Phe His Leu Ile Gly Leu Gly Ser Asp Ala Val Asp 
    290                 295                 300                 


Glu Met Gly Gln Gln Val Phe Asp Glu Phe His Leu Leu Pro Gln Asp 
305                 310                 315                 320 


Gly Ser Leu Phe Asp Arg Leu Ser Phe Leu Lys Asp Ile Cys Asp Lys 
                325                 330                 335     


Asn Asn Pro Ala Val Phe Tyr Met Pro Ser Ile Gly Met Asp Leu Thr 
            340                 345                 350         


Thr Ile Phe Ala Ser Asn Thr Arg Leu Ala Pro Ile Gln Ala Val Ala 
        355                 360                 365             


Leu Gly His Pro Ala Thr Thr His Ser Asp Phe Ile Glu Tyr Val Ile 
    370                 375                 380                 


Val Glu Asp Asp Tyr Val Gly Ser Glu Ser Cys Phe Ser Glu Gln Leu 
385                 390                 395                 400 


Leu Arg Leu Pro Lys Asp Ala Leu Pro Tyr Val Pro Ser Ala Leu Ala 
                405                 410                 415     


Pro Gln Asn Val Val Tyr Asn Leu Arg Glu Asn Pro Glu Val Ile His 
            420                 425                 430         


Ile Gly Ile Ala Ser Thr Thr Met Lys Leu Asn Pro Tyr Phe Leu Glu 
        435                 440                 445             


Ala Leu Lys Ala Ile Arg Asp Arg Ala Lys Val Lys Thr His Phe His 
    450                 455                 460                 


Phe Ala Leu Gly Gln Ser Ser Gly Ile Thr His Pro Tyr Val Glu Arg 
465                 470                 475                 480 


Phe Ile Lys Ser Tyr Leu Gly Asn Asp Ala Thr Ala His Pro His Ser 
                485                 490                 495     


Pro Tyr Asp Glu Tyr Leu Asn Ile Leu His Asn Cys Asp Met Met Leu 
            500                 505                 510         


Asn Pro Phe Pro Phe Gly Asn Thr Asn Gly Ile Ile Asp Met Val Thr 
        515                 520                 525             


Leu Gly Leu Val Gly Val Cys Lys Thr Gly Pro Glu Val His Glu His 
    530                 535                 540                 


Ile Asp Glu Gly Leu Phe Lys Arg Leu Gly Leu Pro Asn Trp Leu Ile 
545                 550                 555                 560 


Thr Gln Thr Ala Glu Glu Tyr Val Thr Gln Ala Ile Arg Leu Ala Glu 
                565                 570                 575     


Asn His Glu Glu Arg Leu Ala Ile Arg Arg Asp Ile Ile Glu Asn Asn 
            580                 585                 590         


Lys Leu Gln Thr Leu Phe Ser Gly Asp Pro Arg Pro Met Gly Gln Ile 
        595                 600                 605             


Phe Leu Ala Lys Val Gln Ala Trp Leu Ala Asp Lys Asn Pro Lys Asn 
    610                 615                 620                 


Ala Glu Val Glu Val Lys Thr Lys Lys Val Arg Lys Ala Ala Thr Ala 
625                 630                 635                 640 


Ser Gln Ser Ala Lys Lys Gln Thr Thr Ser Lys Thr Gln Thr Ala Lys 
                645                 650                 655     


Ala Glu Lys Asp Asn Ala Ala Lys Thr Glu Thr Lys Ser 
            660                 665                 


<210>  8
<211>  669
<212>  PRT
<213>  Artificial

<220>
<223>  Modified Mannheimia haemolytica NGT

<400>  8

Met Ser Ala Glu Asn Met Pro Ser Val Ile Arg Phe Glu Gln Ala Val 
1               5                   10                  15      


Ala Lys Lys Asp Tyr Glu Ser Ala Cys Thr Glu Leu Leu Ser Ile Leu 
            20                  25                  30          


Ser Lys Leu Asp Ser Asn Phe Gly Gly Ile Ser Asn Ile Glu Leu Asn 
        35                  40                  45              


Met Pro Glu Gln Ile Glu Asn Leu Glu Asn Asp Lys Ala Ile Tyr Phe 
    50                  55                  60                  


Cys Thr Arg Met Ala Val Ala Ile Thr Arg Leu Phe Glu Asp Pro Ala 
65                  70                  75                  80  


Leu Glu Ile Ser Glu His Gly Ala Met Arg Phe Leu Thr Leu Gln Arg 
                85                  90                  95      


Trp Ile Ala Leu Ile Phe Ala Ser Ser Pro Tyr Val Asn Ala Asp His 
            100                 105                 110         


Ile Leu Arg Thr Tyr Asn Arg Asn Lys Glu Ser Ala Asn Pro Asn Thr 
        115                 120                 125             


Val Asp Leu Asp Ala Thr Leu Gln Ala Leu Ile Lys Phe Cys Ile Leu 
    130                 135                 140                 


Tyr Leu Pro Glu Ser Asn Ile Leu Leu Asn Leu Asp Ala Ala Trp Asn 
145                 150                 155                 160 


Ala Ser Ser Asp Leu Thr Ala Ser Leu Cys Phe Ala Leu Gln Ser Pro 
                165                 170                 175     


Arg Phe Ile Gly Thr Ser Ser Ala Phe Ala Lys Arg Ala Ala Ile Leu 
            180                 185                 190         


Gln Trp Phe Pro Glu Lys Leu Ala Gln Ile Glu Asn Leu Asn Lys Leu 
        195                 200                 205             


Pro Ser Ala Ile Ser His Asp Val Tyr Met His Cys Ser Tyr Asp Ile 
    210                 215                 220                 


Glu Ala Asn Lys His Asn Val Lys Arg Ser Leu Asn Ala Val Ile Arg 
225                 230                 235                 240 


Arg His Leu Leu Ser Val Gly Trp Glu Asp Arg Lys Ile Glu Gln Leu 
                245                 250                 255     


Gly Thr Arg Asn Asn Lys Pro Val Met Val Val Leu Leu Glu His Phe 
            260                 265                 270         


His Ser Ser His Ser Ile Tyr Arg Thr His Ser Thr Ser Met Val Ala 
        275                 280                 285             


Ala Arg Glu His Phe His Leu Ile Gly Leu Gly Ser Asp Ala Val Asp 
    290                 295                 300                 


Glu Met Gly Gln Gln Val Phe Asp Glu Phe His Leu Leu Pro Gln Asp 
305                 310                 315                 320 


Gly Ser Leu Phe Asp Arg Leu Ser Phe Leu Lys Asp Ile Cys Asp Lys 
                325                 330                 335     


Asn Asn Pro Ala Val Phe Tyr Met Pro Ser Ile Gly Met Asp Leu Thr 
            340                 345                 350         


Thr Ile Phe Ala Ser Asn Thr Arg Leu Ala Pro Ile Gln Ala Val Ala 
        355                 360                 365             


Leu Gly His Pro Ala Thr Thr His Ser Asp Phe Ile Glu Tyr Val Ile 
    370                 375                 380                 


Val Glu Asp Asp Tyr Val Gly Ser Glu Ser Cys Phe Ser Glu Gln Leu 
385                 390                 395                 400 


Leu Arg Leu Pro Lys Asp Ala Leu Pro Tyr Val Pro Ser Ala Leu Ala 
                405                 410                 415     


Pro Gln Asn Val Val Tyr Asn Leu Arg Glu Asn Pro Glu Val Ile His 
            420                 425                 430         


Ile Gly Ile Ala Ser Thr Thr Met Lys Leu Asn Pro Tyr Phe Leu Glu 
        435                 440                 445             


Ala Leu Lys Ala Ile Arg Asp Arg Ala Lys Val Lys Thr His Phe His 
    450                 455                 460                 


Phe Ala Leu Gly Ala Ser Ser Gly Ile Thr His Pro Tyr Val Glu Arg 
465                 470                 475                 480 


Phe Ile Lys Ser Tyr Leu Gly Asn Asp Ala Thr Ala His Pro His Ser 
                485                 490                 495     


Pro Tyr Asp Glu Tyr Leu Asn Ile Leu His Asn Cys Asp Met Met Leu 
            500                 505                 510         


Asn Pro Phe Pro Phe Gly Asn Thr Asn Gly Ile Ile Asp Met Val Thr 
        515                 520                 525             


Leu Gly Leu Val Gly Val Cys Lys Thr Gly Pro Glu Val His Glu His 
    530                 535                 540                 


Ile Asp Glu Gly Leu Phe Lys Arg Leu Gly Leu Pro Asn Trp Leu Ile 
545                 550                 555                 560 


Thr Gln Thr Ala Glu Glu Tyr Val Thr Gln Ala Ile Arg Leu Ala Glu 
                565                 570                 575     


Asn His Glu Glu Arg Leu Ala Ile Arg Arg Asp Ile Ile Glu Asn Asn 
            580                 585                 590         


Lys Leu Gln Thr Leu Phe Ser Gly Asp Pro Arg Pro Met Gly Gln Ile 
        595                 600                 605             


Phe Leu Ala Lys Val Gln Ala Trp Leu Ala Asp Lys Asn Pro Lys Asn 
    610                 615                 620                 


Ala Glu Val Glu Val Lys Thr Lys Lys Val Arg Lys Ala Ala Thr Ala 
625                 630                 635                 640 


Ser Gln Ser Ala Lys Lys Gln Thr Thr Ser Lys Thr Gln Thr Ala Lys 
                645                 650                 655     


Ala Glu Lys Asp Asn Ala Ala Lys Thr Glu Thr Lys Ser 
            660                 665                 


<210>  9
<211>  654
<212>  PRT
<213>  Haemophilus dureyi

<400>  9

Met Glu Leu His Ser Pro Ser Leu Glu Lys Phe Glu Ala Ala Val Ile 
1               5                   10                  15      


Glu Lys Asp Tyr Glu Leu Ala Cys Thr Glu Leu Leu Ala Ile Leu Asp 
            20                  25                  30          


Lys Leu Asp Asn Asn Phe Gly Thr Leu Gln Asp Ile Glu Phe Ala Tyr 
        35                  40                  45              


Pro Pro Gln Leu Glu Asp Leu Glu Gln Asp Lys Val Val Tyr Phe Cys 
    50                  55                  60                  


Thr Arg Met Ala Thr Val Ile Thr Thr Leu Phe Thr Asp Val Glu Phe 
65                  70                  75                  80  


Ala Ile Ser Ser Ala Gly Ala Gln Arg Phe Leu Val Phe Gln Arg Trp 
                85                  90                  95      


Leu Ser Phe Ile Phe Ala Ser Ser Pro Phe Ile Asn Ala Asp His Ile 
            100                 105                 110         


Leu Gln Ser Tyr Asn Cys Asn Pro Asp Arg Asp Ile Glu Asp Asp Ile 
        115                 120                 125             


His Leu Ala Ala Thr Lys Glu Ala Leu Ile Lys Phe Cys Val Met Tyr 
    130                 135                 140                 


Leu Pro Glu Ser Asn Leu Lys Leu Asn Leu Asp Ala Ala Trp Asn Val 
145                 150                 155                 160 


Asp Pro Glu Leu Cys Ala Ser Leu Cys Phe Ala Leu Gln Ser Pro Arg 
                165                 170                 175     


Phe Leu Gly Thr Val Ala Ala Tyr Ser Lys Arg Ser Ala Ile Leu Gln 
            180                 185                 190         


Trp Phe Pro Glu His Leu Ala Gln Leu Ala Asn Leu Asp Asn Ile Pro 
        195                 200                 205             


Ser Ala Ile Ser His Asp Val Tyr Met His Cys Ser Tyr Asp Ile Ala 
    210                 215                 220                 


Glu Asn Lys His Ala Val Lys Lys Ala Leu Asn Gln Val Ile Arg Arg 
225                 230                 235                 240 


His Val Val Asn Glu Tyr Gly Trp Gln Asp Arg Asp Thr Thr Arg Ile 
                245                 250                 255     


Gly Tyr Arg Asn Asp Lys Pro Val Met Val Val Leu Leu Glu His Phe 
            260                 265                 270         


His Ser Ala His Ser Ile Tyr Arg Thr His Ser Thr Ser Met Ile Ala 
        275                 280                 285             


Ala Arg Glu His Phe Tyr Leu Ile Gly Leu Gly Ser Lys Ala Val Asp 
    290                 295                 300                 


Ala Asn Gly Gln Ala Val Phe Asp Glu Phe His Leu Leu Glu Asp Asp 
305                 310                 315                 320 


Asn Met Lys Asp Lys Leu Asp His Ile Arg Ser Ile Cys Glu Gln Asn 
                325                 330                 335     


Gly Ala Ala Ile Leu Tyr Met Pro Ser Val Gly Met Asp Leu Ser Thr 
            340                 345                 350         


Ile Phe Val Ser Asn Thr Arg Leu Ala Pro Ile Gln Val Ile Ala Leu 
        355                 360                 365             


Gly His Pro Ala Thr Thr Tyr Ser Glu Phe Ile Asp Tyr Val Ile Val 
    370                 375                 380                 


Glu Glu Asp Tyr Ile Gly Ser Glu Ala Cys Phe Ser Glu Thr Leu Leu 
385                 390                 395                 400 


Pro Leu Pro Lys Asp Ala Leu Pro Tyr Val Pro Ser Ala Leu Ala Pro 
                405                 410                 415     


Glu Lys Val Glu Tyr Leu Leu Arg Glu Asn Pro Glu Val Val Asn Ile 
            420                 425                 430         


Gly Ile Ala Ala Thr Thr Met Lys Leu Asn Pro Tyr Phe Leu Asp Ala 
        435                 440                 445             


Leu Lys Val Ile Arg Asp Arg Ala Lys Val Lys Ile His Phe His Phe 
    450                 455                 460                 


Ala Leu Gly Gln Ser Thr Gly Val Thr His Pro His Ile Ala Arg Phe 
465                 470                 475                 480 


Ile Lys Ser Tyr Leu Gly Asp Ser Ala Thr Ala Tyr Pro His Ala Pro 
                485                 490                 495     


Tyr His Gln Tyr Leu Thr Val Leu His Asn Cys Asp Met Met Leu Asn 
            500                 505                 510         


Pro Phe Pro Phe Gly Asn Thr Asn Gly Ile Ile Asp Met Val Thr Leu 
        515                 520                 525             


Gly Leu Val Gly Ile Cys Lys Thr Gly Asp Glu Val His Glu His Ile 
    530                 535                 540                 


Asp Glu Gly Leu Phe Lys Arg Leu Gly Leu Pro Glu Trp Leu Ile Ala 
545                 550                 555                 560 


Asp Thr Val Asp Glu Tyr Ile Glu Cys Ala Leu Arg Leu Ala Glu Asn 
                565                 570                 575     


His Thr Glu Arg Leu Ala Leu Arg Arg His Ile Ile Glu Asn Asn Gly 
            580                 585                 590         


Leu Ala Thr Leu Phe Thr Gly Asp Pro Ser Pro Met Gly Ser Val Leu 
        595                 600                 605             


Leu Ala Lys Leu Asn Glu Trp Arg Glu Gln Gln Lys Thr Val Ala Pro 
    610                 615                 620                 


Leu Lys Lys Thr Lys Lys Val Ala Lys Lys Ala Thr Glu Thr Asn Lys 
625                 630                 635                 640 


Ser Val Thr Lys Lys Pro Val Ala Lys Lys Lys Arg Ser Ser 
                645                 650                 


<210>  10
<211>  654
<212>  PRT
<213>  Artificial

<220>
<223>  Modified Haemophilus dureyi NGT

<400>  10

Met Glu Leu His Ser Pro Ser Leu Glu Lys Phe Glu Ala Ala Val Ile 
1               5                   10                  15      


Glu Lys Asp Tyr Glu Leu Ala Cys Thr Glu Leu Leu Ala Ile Leu Asp 
            20                  25                  30          


Lys Leu Asp Asn Asn Phe Gly Thr Leu Gln Asp Ile Glu Phe Ala Tyr 
        35                  40                  45              


Pro Pro Gln Leu Glu Asp Leu Glu Gln Asp Lys Val Val Tyr Phe Cys 
    50                  55                  60                  


Thr Arg Met Ala Thr Val Ile Thr Thr Leu Phe Thr Asp Val Glu Phe 
65                  70                  75                  80  


Ala Ile Ser Ser Ala Gly Ala Gln Arg Phe Leu Val Phe Gln Arg Trp 
                85                  90                  95      


Leu Ser Phe Ile Phe Ala Ser Ser Pro Phe Ile Asn Ala Asp His Ile 
            100                 105                 110         


Leu Gln Ser Tyr Asn Cys Asn Pro Asp Arg Asp Ile Glu Asp Asp Ile 
        115                 120                 125             


His Leu Ala Ala Thr Lys Glu Ala Leu Ile Lys Phe Cys Val Met Tyr 
    130                 135                 140                 


Leu Pro Glu Ser Asn Leu Lys Leu Asn Leu Asp Ala Ala Trp Asn Val 
145                 150                 155                 160 


Asp Pro Glu Leu Cys Ala Ser Leu Cys Phe Ala Leu Gln Ser Pro Arg 
                165                 170                 175     


Phe Leu Gly Thr Val Ala Ala Tyr Ser Lys Arg Ser Ala Ile Leu Gln 
            180                 185                 190         


Trp Phe Pro Glu His Leu Ala Gln Leu Ala Asn Leu Asp Asn Ile Pro 
        195                 200                 205             


Ser Ala Ile Ser His Asp Val Tyr Met His Cys Ser Tyr Asp Ile Ala 
    210                 215                 220                 


Glu Asn Lys His Ala Val Lys Lys Ala Leu Asn Gln Val Ile Arg Arg 
225                 230                 235                 240 


His Val Val Asn Glu Tyr Gly Trp Gln Asp Arg Asp Thr Thr Arg Ile 
                245                 250                 255     


Gly Tyr Arg Asn Asp Lys Pro Val Met Val Val Leu Leu Glu His Phe 
            260                 265                 270         


His Ser Ala His Ser Ile Tyr Arg Thr His Ser Thr Ser Met Ile Ala 
        275                 280                 285             


Ala Arg Glu His Phe Tyr Leu Ile Gly Leu Gly Ser Lys Ala Val Asp 
    290                 295                 300                 


Ala Asn Gly Gln Ala Val Phe Asp Glu Phe His Leu Leu Glu Asp Asp 
305                 310                 315                 320 


Asn Met Lys Asp Lys Leu Asp His Ile Arg Ser Ile Cys Glu Gln Asn 
                325                 330                 335     


Gly Ala Ala Ile Leu Tyr Met Pro Ser Val Gly Met Asp Leu Ser Thr 
            340                 345                 350         


Ile Phe Val Ser Asn Thr Arg Leu Ala Pro Ile Gln Val Ile Ala Leu 
        355                 360                 365             


Gly His Pro Ala Thr Thr Tyr Ser Glu Phe Ile Asp Tyr Val Ile Val 
    370                 375                 380                 


Glu Glu Asp Tyr Ile Gly Ser Glu Ala Cys Phe Ser Glu Thr Leu Leu 
385                 390                 395                 400 


Pro Leu Pro Lys Asp Ala Leu Pro Tyr Val Pro Ser Ala Leu Ala Pro 
                405                 410                 415     


Glu Lys Val Glu Tyr Leu Leu Arg Glu Asn Pro Glu Val Val Asn Ile 
            420                 425                 430         


Gly Ile Ala Ala Thr Thr Met Lys Leu Asn Pro Tyr Phe Leu Asp Ala 
        435                 440                 445             


Leu Lys Val Ile Arg Asp Arg Ala Lys Val Lys Ile His Phe His Phe 
    450                 455                 460                 


Ala Leu Gly Ala Ser Thr Gly Val Thr His Pro His Ile Ala Arg Phe 
465                 470                 475                 480 


Ile Lys Ser Tyr Leu Gly Asp Ser Ala Thr Ala Tyr Pro His Ala Pro 
                485                 490                 495     


Tyr His Gln Tyr Leu Thr Val Leu His Asn Cys Asp Met Met Leu Asn 
            500                 505                 510         


Pro Phe Pro Phe Gly Asn Thr Asn Gly Ile Ile Asp Met Val Thr Leu 
        515                 520                 525             


Gly Leu Val Gly Ile Cys Lys Thr Gly Asp Glu Val His Glu His Ile 
    530                 535                 540                 


Asp Glu Gly Leu Phe Lys Arg Leu Gly Leu Pro Glu Trp Leu Ile Ala 
545                 550                 555                 560 


Asp Thr Val Asp Glu Tyr Ile Glu Cys Ala Leu Arg Leu Ala Glu Asn 
                565                 570                 575     


His Thr Glu Arg Leu Ala Leu Arg Arg His Ile Ile Glu Asn Asn Gly 
            580                 585                 590         


Leu Ala Thr Leu Phe Thr Gly Asp Pro Ser Pro Met Gly Ser Val Leu 
        595                 600                 605             


Leu Ala Lys Leu Asn Glu Trp Arg Glu Gln Gln Lys Thr Val Ala Pro 
    610                 615                 620                 


Leu Lys Lys Thr Lys Lys Val Ala Lys Lys Ala Thr Glu Thr Asn Lys 
625                 630                 635                 640 


Ser Val Thr Lys Lys Pro Val Ala Lys Lys Lys Arg Ser Ser 
                645                 650                 


<210>  11
<211>  690
<212>  PRT
<213>  Bibersteinia trehalosi

<400>  11

Met Ser Gln Glu Gln Lys Thr Pro Ser Val Ile Arg Phe Glu Gln Ala 
1               5                   10                  15      


Val Lys Ala Lys Gln Tyr Glu Ser Ala Cys Asn Glu Leu Leu Asp Ile 
            20                  25                  30          


Leu Ser Gln Ile Asp Ser Asn Phe Gly Gly Ile Asn Gly Ile Glu Phe 
        35                  40                  45              


Asn Cys Pro Glu Gln Leu Asn Asn Pro Asn Leu Ser Lys Glu Lys Thr 
    50                  55                  60                  


Ile Tyr Phe Ser Thr Arg Met Ala Asp Leu Ile Thr Glu Leu Phe Ser 
65                  70                  75                  80  


Asp Glu Ser Leu Ser Leu Thr Val Gly Gly Ala Val Arg Phe Phe Ser 
                85                  90                  95      


Tyr Gln Arg Trp Ile Ala Leu Leu Phe Ala Cys Ser Pro Tyr Ile Asn 
            100                 105                 110         


Ser Asp His Ile Leu Gln Val Tyr Asn Arg Asn Pro Asp Lys Ser Asn 
        115                 120                 125             


Pro Asn Ser Val His Leu Ser Ala Asn Pro Asn Asp Leu Val Lys Phe 
    130                 135                 140                 


Cys Ile Met Tyr Leu Pro Glu Ser Asn Ile Ser Leu Asn Leu Asp Ala 
145                 150                 155                 160 


Ile Trp Gln Leu Asn Pro Thr Leu Cys Ala Ser Met Cys Phe Ala Leu 
                165                 170                 175     


Gln Ser Pro Arg Phe Ile Gly Thr Lys Glu Ala Phe Gly Lys Arg Gly 
            180                 185                 190         


Ala Ile Leu Gln Trp Phe Pro Glu Lys Leu Ala Gln Leu Pro Asn Leu 
        195                 200                 205             


Asp Asn Leu Pro Ser Ser Ile Ser His Asp Val Tyr Met His Cys Ser 
    210                 215                 220                 


Tyr Asp Val Ala Ala Asn Lys His Asp Val Lys Arg Ala Leu Asn Gln 
225                 230                 235                 240 


Val Met Arg Arg His Leu Val Thr Ser Gly Trp Val Asp Arg Asp Ile 
                245                 250                 255     


Ser Lys Ile Gly Lys Thr Asn Gly Lys Pro Val Met Val Val Leu Leu 
            260                 265                 270         


Glu His Phe His Ser Ala His Ser Ile Tyr Arg Thr His Ser Thr Ser 
        275                 280                 285             


Met Arg Ala Ala Arg Glu His Phe His Leu Ile Gly Ile Gly Gly Ser 
    290                 295                 300                 


Ala Val Asp Lys Ala Gly Gln Glu Val Phe Asp Asp Phe Arg Leu Val 
305                 310                 315                 320 


Glu Gly Asn Thr Ile Phe Glu Lys Leu Ser Phe Val Lys Arg Leu Cys 
                325                 330                 335     


Glu Glu Tyr Gly Ala Ala Ile Phe Tyr Met Pro Ser Ile Gly Met Asp 
            340                 345                 350         


Leu Thr Thr Ile Phe Ala Ser Asn Thr Arg Leu Ala Pro Ile Gln Ala 
        355                 360                 365             


Ile Ala Leu Gly His Pro Gly Thr Thr His Ser Glu Phe Ile Glu Tyr 
    370                 375                 380                 


Val Val Val Glu Asp Asp Tyr Val Gly Ser Glu Ala Cys Phe Ser Glu 
385                 390                 395                 400 


Lys Leu Leu Arg Leu Pro Lys Asp Ala Leu Pro Tyr Val Pro Ser Ala 
                405                 410                 415     


Leu Ala Pro Ala Ser Val Glu Tyr Arg Leu Arg Glu Asn Pro Glu Val 
            420                 425                 430         


Val Asn Ile Gly Ile Ala Ser Thr Thr Met Lys Leu Asn Pro Tyr Phe 
        435                 440                 445             


Leu Asp Ala Leu Lys Ala Ile Arg Asp Arg Ala Lys Val Lys Val His 
    450                 455                 460                 


Phe His Phe Ala Leu Gly Gln Ser Ser Gly Ile Thr His Pro Tyr Val 
465                 470                 475                 480 


Glu Arg Phe Ile Lys Ser His Leu Gly Asp Ser Ala Thr Ala His Pro 
                485                 490                 495     


His Ser Pro Tyr His Gln Tyr Met Gln Ile Leu His Asn Cys Asp Met 
            500                 505                 510         


Leu Val Asn Pro Phe Pro Phe Gly Asn Thr Asn Gly Ile Ile Asp Met 
        515                 520                 525             


Val Thr Leu Gly Leu Val Gly Ile Cys Lys Thr Gly Pro Glu Val His 
    530                 535                 540                 


Glu His Ile Asp Glu Gly Leu Phe Lys Arg Leu Gly Leu Pro Glu Trp 
545                 550                 555                 560 


Leu Ile Ala Asn Thr Val Asp Glu Tyr Val Glu Arg Ala Val Arg Leu 
                565                 570                 575     


Ala Glu Asn His Ala Glu Arg Leu Ala Leu Arg Arg His Ile Ile Glu 
            580                 585                 590         


Asn Asn Gly Leu Gln Thr Leu Phe Thr Gly Asp Pro Lys Pro Met Gly 
        595                 600                 605             


Gln Val Phe Val Gln Lys Leu Asn Glu Trp Ala Gly Leu His Asn Ile 
    610                 615                 620                 


Asp Val Ser Asp Phe Ala Phe Ala Gln Ser Ser Gly Lys Lys Val Thr 
625                 630                 635                 640 


Lys Ser Ala Lys Thr Ala Ala Lys Lys Thr Val Lys Val Thr Val Lys 
                645                 650                 655     


Lys Ser Ala Gln Pro Lys Glu Ser Thr Lys Thr Lys Ser Lys Thr Glu 
            660                 665                 670         


Lys Lys Lys Thr Ser Ser Val Lys Asp Ala Ala Lys Thr Ser Lys Lys 
        675                 680                 685             


Lys Ala 
    690 


<210>  12
<211>  690
<212>  PRT
<213>  Artificial

<220>
<223>  Modified Bibersteinia trehalosi NGT

<400>  12

Met Ser Gln Glu Gln Lys Thr Pro Ser Val Ile Arg Phe Glu Gln Ala 
1               5                   10                  15      


Val Lys Ala Lys Gln Tyr Glu Ser Ala Cys Asn Glu Leu Leu Asp Ile 
            20                  25                  30          


Leu Ser Gln Ile Asp Ser Asn Phe Gly Gly Ile Asn Gly Ile Glu Phe 
        35                  40                  45              


Asn Cys Pro Glu Gln Leu Asn Asn Pro Asn Leu Ser Lys Glu Lys Thr 
    50                  55                  60                  


Ile Tyr Phe Ser Thr Arg Met Ala Asp Leu Ile Thr Glu Leu Phe Ser 
65                  70                  75                  80  


Asp Glu Ser Leu Ser Leu Thr Val Gly Gly Ala Val Arg Phe Phe Ser 
                85                  90                  95      


Tyr Gln Arg Trp Ile Ala Leu Leu Phe Ala Cys Ser Pro Tyr Ile Asn 
            100                 105                 110         


Ser Asp His Ile Leu Gln Val Tyr Asn Arg Asn Pro Asp Lys Ser Asn 
        115                 120                 125             


Pro Asn Ser Val His Leu Ser Ala Asn Pro Asn Asp Leu Val Lys Phe 
    130                 135                 140                 


Cys Ile Met Tyr Leu Pro Glu Ser Asn Ile Ser Leu Asn Leu Asp Ala 
145                 150                 155                 160 


Ile Trp Gln Leu Asn Pro Thr Leu Cys Ala Ser Met Cys Phe Ala Leu 
                165                 170                 175     


Gln Ser Pro Arg Phe Ile Gly Thr Lys Glu Ala Phe Gly Lys Arg Gly 
            180                 185                 190         


Ala Ile Leu Gln Trp Phe Pro Glu Lys Leu Ala Gln Leu Pro Asn Leu 
        195                 200                 205             


Asp Asn Leu Pro Ser Ser Ile Ser His Asp Val Tyr Met His Cys Ser 
    210                 215                 220                 


Tyr Asp Val Ala Ala Asn Lys His Asp Val Lys Arg Ala Leu Asn Gln 
225                 230                 235                 240 


Val Met Arg Arg His Leu Val Thr Ser Gly Trp Val Asp Arg Asp Ile 
                245                 250                 255     


Ser Lys Ile Gly Lys Thr Asn Gly Lys Pro Val Met Val Val Leu Leu 
            260                 265                 270         


Glu His Phe His Ser Ala His Ser Ile Tyr Arg Thr His Ser Thr Ser 
        275                 280                 285             


Met Arg Ala Ala Arg Glu His Phe His Leu Ile Gly Ile Gly Gly Ser 
    290                 295                 300                 


Ala Val Asp Lys Ala Gly Gln Glu Val Phe Asp Asp Phe Arg Leu Val 
305                 310                 315                 320 


Glu Gly Asn Thr Ile Phe Glu Lys Leu Ser Phe Val Lys Arg Leu Cys 
                325                 330                 335     


Glu Glu Tyr Gly Ala Ala Ile Phe Tyr Met Pro Ser Ile Gly Met Asp 
            340                 345                 350         


Leu Thr Thr Ile Phe Ala Ser Asn Thr Arg Leu Ala Pro Ile Gln Ala 
        355                 360                 365             


Ile Ala Leu Gly His Pro Gly Thr Thr His Ser Glu Phe Ile Glu Tyr 
    370                 375                 380                 


Val Val Val Glu Asp Asp Tyr Val Gly Ser Glu Ala Cys Phe Ser Glu 
385                 390                 395                 400 


Lys Leu Leu Arg Leu Pro Lys Asp Ala Leu Pro Tyr Val Pro Ser Ala 
                405                 410                 415     


Leu Ala Pro Ala Ser Val Glu Tyr Arg Leu Arg Glu Asn Pro Glu Val 
            420                 425                 430         


Val Asn Ile Gly Ile Ala Ser Thr Thr Met Lys Leu Asn Pro Tyr Phe 
        435                 440                 445             


Leu Asp Ala Leu Lys Ala Ile Arg Asp Arg Ala Lys Val Lys Val His 
    450                 455                 460                 


Phe His Phe Ala Leu Gly Ala Ser Ser Gly Ile Thr His Pro Tyr Val 
465                 470                 475                 480 


Glu Arg Phe Ile Lys Ser His Leu Gly Asp Ser Ala Thr Ala His Pro 
                485                 490                 495     


His Ser Pro Tyr His Gln Tyr Met Gln Ile Leu His Asn Cys Asp Met 
            500                 505                 510         


Leu Val Asn Pro Phe Pro Phe Gly Asn Thr Asn Gly Ile Ile Asp Met 
        515                 520                 525             


Val Thr Leu Gly Leu Val Gly Ile Cys Lys Thr Gly Pro Glu Val His 
    530                 535                 540                 


Glu His Ile Asp Glu Gly Leu Phe Lys Arg Leu Gly Leu Pro Glu Trp 
545                 550                 555                 560 


Leu Ile Ala Asn Thr Val Asp Glu Tyr Val Glu Arg Ala Val Arg Leu 
                565                 570                 575     


Ala Glu Asn His Ala Glu Arg Leu Ala Leu Arg Arg His Ile Ile Glu 
            580                 585                 590         


Asn Asn Gly Leu Gln Thr Leu Phe Thr Gly Asp Pro Lys Pro Met Gly 
        595                 600                 605             


Gln Val Phe Val Gln Lys Leu Asn Glu Trp Ala Gly Leu His Asn Ile 
    610                 615                 620                 


Asp Val Ser Asp Phe Ala Phe Ala Gln Ser Ser Gly Lys Lys Val Thr 
625                 630                 635                 640 


Lys Ser Ala Lys Thr Ala Ala Lys Lys Thr Val Lys Val Thr Val Lys 
                645                 650                 655     


Lys Ser Ala Gln Pro Lys Glu Ser Thr Lys Thr Lys Ser Lys Thr Glu 
            660                 665                 670         


Lys Lys Lys Thr Ser Ser Val Lys Asp Ala Ala Lys Thr Ser Lys Lys 
        675                 680                 685             


Lys Ala 
    690 


<210>  13
<211>  621
<212>  PRT
<213>  Aggregatibacter aphrophilus

<400>  13

Met Ser Glu Lys Lys Asn Pro Ser Val Ile Gln Phe Glu Lys Ala Ile 
1               5                   10                  15      


Arg Glu Lys Asn Tyr Glu Ala Ala Cys Thr Glu Leu Leu Asp Ile Leu 
            20                  25                  30          


Asn Lys Ile Asp Thr Asn Phe Gly Asp Ile Glu Gly Ile Asp Phe Asp 
        35                  40                  45              


Tyr Pro Gln Gln Leu Lys Thr Leu Met Gln Glu Arg Ile Val Tyr Phe 
    50                  55                  60                  


Cys Thr Arg Met Ala Asn Ala Ile Thr Gln Leu Phe Cys Asp Pro Gln 
65                  70                  75                  80  


Phe Ser Leu Ser Glu Ser Gly Ala Asn Arg Phe Phe Val Val Gln Arg 
                85                  90                  95      


Trp Leu Asn Leu Ile Phe Ala Ser Ser Pro Tyr Ile Asn Ala Asp His 
            100                 105                 110         


Ile Leu Gln Thr Tyr Asn Cys Asn Pro Glu Arg Asp Ser Ile Tyr Asp 
        115                 120                 125             


Ile Tyr Leu Glu Pro Asn Lys Asn Val Leu Met Lys Phe Ala Val Leu 
    130                 135                 140                 


Tyr Leu Pro Glu Ser Asn Val Asn Leu Asn Leu Asp Thr Met Trp Glu 
145                 150                 155                 160 


Thr Asp Lys Asn Ile Cys Gly Ser Leu Cys Phe Ala Leu Gln Ser Pro 
                165                 170                 175     


Arg Phe Ile Gly Thr Pro Ala Ala Phe Ser Lys Arg Ser Thr Ile Leu 
            180                 185                 190         


Gln Trp Phe Pro Ala Lys Leu Glu Gln Phe His Val Leu Asp Asp Leu 
        195                 200                 205             


Pro Ser Asn Ile Ser His Asp Val Tyr Met His Cys Ser Tyr Asp Thr 
    210                 215                 220                 


Ala Glu Asn Lys His Asn Val Lys Lys Ala Leu Asn Gln Val Ile Arg 
225                 230                 235                 240 


Ser His Leu Leu Lys Cys Gly Trp Gln Asp Arg Gln Ile Thr Gln Ile 
                245                 250                 255     


Gly Met Arg Asn Gly Lys Pro Val Met Val Val Val Leu Glu His Phe 
            260                 265                 270         


His Ser Ser His Ser Ile Tyr Arg Thr His Ser Thr Ser Met Ile Ala 
        275                 280                 285             


Ala Arg Glu Gln Phe Tyr Leu Ile Gly Leu Gly Asn Asn Ala Val Asp 
    290                 295                 300                 


Gln Ala Gly Arg Asp Val Phe Asp Glu Phe His Glu Phe Asp Asp Ser 
305                 310                 315                 320 


Asn Ile Leu Lys Lys Leu Ala Phe Leu Lys Glu Met Cys Glu Lys Asn 
                325                 330                 335     


Asp Ala Ala Val Leu Tyr Met Pro Ser Ile Gly Met Asp Leu Ala Thr 
            340                 345                 350         


Ile Phe Val Ser Asn Ala Arg Phe Ala Pro Ile Gln Val Ile Ala Leu 
        355                 360                 365             


Gly His Pro Ala Thr Thr His Ser Glu Phe Ile Glu Tyr Val Ile Val 
    370                 375                 380                 


Glu Asp Asp Tyr Val Gly Ser Val Ser Cys Phe Ser Glu Thr Leu Leu 
385                 390                 395                 400 


Arg Leu Pro Lys Asp Ala Leu Pro Tyr Val Pro Ser Ser Leu Ala Pro 
                405                 410                 415     


Thr Asp Val Gln Tyr Val Leu Gln Glu Thr Pro Glu Val Val Asn Ile 
            420                 425                 430         


Gly Ile Ala Ala Thr Thr Met Lys Leu Asn Pro Tyr Phe Leu Glu Thr 
        435                 440                 445             


Leu Lys Thr Ile Arg Asp Arg Ala Lys Val Lys Val His Phe His Phe 
    450                 455                 460                 


Ala Leu Gly Gln Ser Ile Gly Ile Thr His Pro Tyr Val Ala Arg Phe 
465                 470                 475                 480 


Ile Arg Ser Tyr Leu Gly Asn Asp Ala Thr Ala His Pro His Ser Pro 
                485                 490                 495     


Tyr Asn Arg Tyr Leu Asp Ile Leu His Asn Cys Asp Met Met Leu Asn 
            500                 505                 510         


Pro Phe Pro Phe Gly Asn Thr Asn Gly Ile Ile Asp Met Val Thr Leu 
        515                 520                 525             


Gly Leu Val Gly Val Cys Lys Thr Gly Pro Glu Val His Glu His Ile 
    530                 535                 540                 


Asp Glu Gly Leu Phe Lys Arg Leu Gly Leu Pro Glu Trp Leu Ile Ala 
545                 550                 555                 560 


Asp Ser Val Glu Asp Tyr Ile Glu Arg Ala Ile Arg Leu Ala Glu Asn 
                565                 570                 575     


His Gln Glu Arg Leu Ala Leu Arg Arg His Ile Ile Glu Asn Asn Gly 
            580                 585                 590         


Leu Lys Thr Leu Phe Ser Gly Asp Pro Ser Pro Met Gly Lys Met Leu 
        595                 600                 605             


Phe Ala Lys Leu Thr Glu Trp Arg Gln Thr Asn Gly Ile 
    610                 615                 620     


<210>  14
<211>  621
<212>  PRT
<213>  Artificial

<220>
<223>  Modified Aggregatibacter aphrophilus NGT

<400>  14

Met Ser Glu Lys Lys Asn Pro Ser Val Ile Gln Phe Glu Lys Ala Ile 
1               5                   10                  15      


Arg Glu Lys Asn Tyr Glu Ala Ala Cys Thr Glu Leu Leu Asp Ile Leu 
            20                  25                  30          


Asn Lys Ile Asp Thr Asn Phe Gly Asp Ile Glu Gly Ile Asp Phe Asp 
        35                  40                  45              


Tyr Pro Gln Gln Leu Lys Thr Leu Met Gln Glu Arg Ile Val Tyr Phe 
    50                  55                  60                  


Cys Thr Arg Met Ala Asn Ala Ile Thr Gln Leu Phe Cys Asp Pro Gln 
65                  70                  75                  80  


Phe Ser Leu Ser Glu Ser Gly Ala Asn Arg Phe Phe Val Val Gln Arg 
                85                  90                  95      


Trp Leu Asn Leu Ile Phe Ala Ser Ser Pro Tyr Ile Asn Ala Asp His 
            100                 105                 110         


Ile Leu Gln Thr Tyr Asn Cys Asn Pro Glu Arg Asp Ser Ile Tyr Asp 
        115                 120                 125             


Ile Tyr Leu Glu Pro Asn Lys Asn Val Leu Met Lys Phe Ala Val Leu 
    130                 135                 140                 


Tyr Leu Pro Glu Ser Asn Val Asn Leu Asn Leu Asp Thr Met Trp Glu 
145                 150                 155                 160 


Thr Asp Lys Asn Ile Cys Gly Ser Leu Cys Phe Ala Leu Gln Ser Pro 
                165                 170                 175     


Arg Phe Ile Gly Thr Pro Ala Ala Phe Ser Lys Arg Ser Thr Ile Leu 
            180                 185                 190         


Gln Trp Phe Pro Ala Lys Leu Glu Gln Phe His Val Leu Asp Asp Leu 
        195                 200                 205             


Pro Ser Asn Ile Ser His Asp Val Tyr Met His Cys Ser Tyr Asp Thr 
    210                 215                 220                 


Ala Glu Asn Lys His Asn Val Lys Lys Ala Leu Asn Gln Val Ile Arg 
225                 230                 235                 240 


Ser His Leu Leu Lys Cys Gly Trp Gln Asp Arg Gln Ile Thr Gln Ile 
                245                 250                 255     


Gly Met Arg Asn Gly Lys Pro Val Met Val Val Val Leu Glu His Phe 
            260                 265                 270         


His Ser Ser His Ser Ile Tyr Arg Thr His Ser Thr Ser Met Ile Ala 
        275                 280                 285             


Ala Arg Glu Gln Phe Tyr Leu Ile Gly Leu Gly Asn Asn Ala Val Asp 
    290                 295                 300                 


Gln Ala Gly Arg Asp Val Phe Asp Glu Phe His Glu Phe Asp Asp Ser 
305                 310                 315                 320 


Asn Ile Leu Lys Lys Leu Ala Phe Leu Lys Glu Met Cys Glu Lys Asn 
                325                 330                 335     


Asp Ala Ala Val Leu Tyr Met Pro Ser Ile Gly Met Asp Leu Ala Thr 
            340                 345                 350         


Ile Phe Val Ser Asn Ala Arg Phe Ala Pro Ile Gln Val Ile Ala Leu 
        355                 360                 365             


Gly His Pro Ala Thr Thr His Ser Glu Phe Ile Glu Tyr Val Ile Val 
    370                 375                 380                 


Glu Asp Asp Tyr Val Gly Ser Val Ser Cys Phe Ser Glu Thr Leu Leu 
385                 390                 395                 400 


Arg Leu Pro Lys Asp Ala Leu Pro Tyr Val Pro Ser Ser Leu Ala Pro 
                405                 410                 415     


Thr Asp Val Gln Tyr Val Leu Gln Glu Thr Pro Glu Val Val Asn Ile 
            420                 425                 430         


Gly Ile Ala Ala Thr Thr Met Lys Leu Asn Pro Tyr Phe Leu Glu Thr 
        435                 440                 445             


Leu Lys Thr Ile Arg Asp Arg Ala Lys Val Lys Val His Phe His Phe 
    450                 455                 460                 


Ala Leu Gly Ala Ser Ile Gly Ile Thr His Pro Tyr Val Ala Arg Phe 
465                 470                 475                 480 


Ile Arg Ser Tyr Leu Gly Asn Asp Ala Thr Ala His Pro His Ser Pro 
                485                 490                 495     


Tyr Asn Arg Tyr Leu Asp Ile Leu His Asn Cys Asp Met Met Leu Asn 
            500                 505                 510         


Pro Phe Pro Phe Gly Asn Thr Asn Gly Ile Ile Asp Met Val Thr Leu 
        515                 520                 525             


Gly Leu Val Gly Val Cys Lys Thr Gly Pro Glu Val His Glu His Ile 
    530                 535                 540                 


Asp Glu Gly Leu Phe Lys Arg Leu Gly Leu Pro Glu Trp Leu Ile Ala 
545                 550                 555                 560 


Asp Ser Val Glu Asp Tyr Ile Glu Arg Ala Ile Arg Leu Ala Glu Asn 
                565                 570                 575     


His Gln Glu Arg Leu Ala Leu Arg Arg His Ile Ile Glu Asn Asn Gly 
            580                 585                 590         


Leu Lys Thr Leu Phe Ser Gly Asp Pro Ser Pro Met Gly Lys Met Leu 
        595                 600                 605             


Phe Ala Lys Leu Thr Glu Trp Arg Gln Thr Asn Gly Ile 
    610                 615                 620     


<210>  15
<211>  619
<212>  PRT
<213>  Yersinia enterocolitica

<400>  15

Met Val Asp Lys Thr Val Glu Val Ser Gln Glu Ala Glu Asn Leu Thr 
1               5                   10                  15      


Ala Phe Ser Leu Pro Tyr Phe Glu Phe Leu Val Cys Val Arg Arg Tyr 
            20                  25                  30          


Glu Glu Ala Gly Arg Leu Leu Ile Leu Met Leu Glu Gln Leu Asp Thr 
        35                  40                  45              


Gln Tyr Gly Arg Trp Asp Val Phe Ser Leu Lys Gln Gln Ser Ile Gln 
    50                  55                  60                  


Gln Gln Glu His Tyr Cys Asn Arg Leu Ala Ala Ala Ile Gly Asn Leu 
65                  70                  75                  80  


Phe Ser Asp Pro Gly Phe Val Leu Ser Glu Lys Gly Phe Leu Gln Leu 
                85                  90                  95      


Ile Asn Phe His Arg Trp Ile Ala Leu Ile Phe Ala Ala Ser Pro Phe 
            100                 105                 110         


Gly His Ala Asp His Val Ile Thr Asn Leu Asn Gln Val Gly Glu Gly 
        115                 120                 125             


Cys Ala His Pro Leu Arg Phe Glu Gln Asn Asn Phe Leu Lys Phe Cys 
    130                 135                 140                 


Val Met Tyr Leu Pro Glu Ser Gly Ile Pro Leu Gln Pro Asp Ile Leu 
145                 150                 155                 160 


Trp Gln Phe Asn Pro Asn Ala Ala Ala Ala Leu Phe Leu Ala Leu Leu 
                165                 170                 175     


Ser Pro Arg Ile Leu Pro Ser Thr Val Gly His Ala Lys Arg Glu Leu 
            180                 185                 190         


Leu Leu Arg Trp Leu Pro Glu Arg Leu Leu Thr Leu Asp Ser Leu Glu 
        195                 200                 205             


His Leu Pro Glu Arg Ile Leu His Asp Val Tyr Met His Cys Ser Tyr 
    210                 215                 220                 


Ala Asp Met Ala Glu Lys His Ala Ile Lys Arg Ser Ile Asn Phe His 
225                 230                 235                 240 


Leu Arg Asn Thr Leu Leu His Asn Gly Leu Ser Asp Asn His Leu Ser 
                245                 250                 255     


Pro Pro Ser Arg Asp Lys Pro Leu Met Leu Val Ile Leu Glu Trp Phe 
            260                 265                 270         


Asn Ser Gly His Ser Ile Tyr Arg Thr His Ser Ser Thr Leu Arg Ala 
        275                 280                 285             


Ala Arg Glu Gln Phe Ser Thr His Gly Ala Thr Ile Ile Asp Ala Thr 
    290                 295                 300                 


Asp Ala Ile Thr Gln Ala Val Phe Asp Asp Phe Thr Glu Val Asn Arg 
305                 310                 315                 320 


Ala Gly Ala Val Glu Ala Ile Val Ala Leu Thr Gln Gln Leu Leu Pro 
                325                 330                 335     


Asp Val Ile Tyr Phe Pro Ser Val Gly Met Phe Pro Leu Thr Ile Ala 
            340                 345                 350         


Leu Thr Asn Leu Arg Leu Ala Pro Leu Gln Val Met Ala Leu Gly His 
        355                 360                 365             


Pro Ala Thr Thr His Ser Asp Tyr Ile Asp Ala Val Leu Val Glu Glu 
    370                 375                 380                 


Asp Tyr Leu Gly Asp Ile Ala Cys Phe Ser Glu Lys Val Val Ser Leu 
385                 390                 395                 400 


Pro Lys Asp Cys Leu Pro Tyr Val Pro Pro Ala Asn Ile Ser Gln Pro 
                405                 410                 415     


Glu Pro Ile Leu His Phe Ala Glu Arg Pro Ala Val His Ile Ala Val 
            420                 425                 430         


Cys Ala Ser Ala Met Lys Ile Asn Pro Arg Phe Leu Ala Thr Cys Ala 
        435                 440                 445             


Glu Ile Thr Arg Gln Thr Ser Thr Ser Val Val Phe His Phe Leu Val 
    450                 455                 460                 


Gly Phe Cys Trp Gly Ile Thr His Arg Val Met Glu Lys Ala Val Asn 
465                 470                 475                 480 


Asp Ile Leu Pro Gln Ala Arg Val Tyr Glu His Leu Gly Tyr Leu Asp 
                485                 490                 495     


Tyr Leu Gln Val Ile Asn Gln Cys Asp Leu Phe Ile Asn Pro Phe Pro 
            500                 505                 510         


Phe Gly Asn Thr Asn Gly Ile Val Asp Thr Val Arg Gln Gly Leu Pro 
        515                 520                 525             


Gly Val Cys Leu Ser Gly Thr Glu Val His Glu His Ile Asp Glu Gly 
    530                 535                 540                 


Leu Phe Arg Arg Leu Gly Leu Asp Glu Glu Leu Ile Ala His Asp Leu 
545                 550                 555                 560 


Ala Glu Tyr Ile Ala Val Thr Val Arg Leu Ile Ser Asp Lys Glu Trp 
                565                 570                 575     


Arg Gln Ser Leu Arg Gln Arg Leu Leu Gln Ile Gln Pro Asp Asn Val 
            580                 585                 590         


Leu Phe Ala Gly Lys Pro Glu Gln Phe Gly Leu Ile Val Arg Gly Leu 
        595                 600                 605             


Leu Ala Asp Lys Lys Ala Ser Asp Lys Gly Gly 
    610                 615                 


<210>  16
<211>  619
<212>  PRT
<213>  Artificial

<220>
<223>  Modified Yersinia enterocolitica NGT

<400>  16

Met Val Asp Lys Thr Val Glu Val Ser Gln Glu Ala Glu Asn Leu Thr 
1               5                   10                  15      


Ala Phe Ser Leu Pro Tyr Phe Glu Phe Leu Val Cys Val Arg Arg Tyr 
            20                  25                  30          


Glu Glu Ala Gly Arg Leu Leu Ile Leu Met Leu Glu Gln Leu Asp Thr 
        35                  40                  45              


Gln Tyr Gly Arg Trp Asp Val Phe Ser Leu Lys Gln Gln Ser Ile Gln 
    50                  55                  60                  


Gln Gln Glu His Tyr Cys Asn Arg Leu Ala Ala Ala Ile Gly Asn Leu 
65                  70                  75                  80  


Phe Ser Asp Pro Gly Phe Val Leu Ser Glu Lys Gly Phe Leu Gln Leu 
                85                  90                  95      


Ile Asn Phe His Arg Trp Ile Ala Leu Ile Phe Ala Ala Ser Pro Phe 
            100                 105                 110         


Gly His Ala Asp His Val Ile Thr Asn Leu Asn Gln Val Gly Glu Gly 
        115                 120                 125             


Cys Ala His Pro Leu Arg Phe Glu Gln Asn Asn Phe Leu Lys Phe Cys 
    130                 135                 140                 


Val Met Tyr Leu Pro Glu Ser Gly Ile Pro Leu Gln Pro Asp Ile Leu 
145                 150                 155                 160 


Trp Gln Phe Asn Pro Asn Ala Ala Ala Ala Leu Phe Leu Ala Leu Leu 
                165                 170                 175     


Ser Pro Arg Ile Leu Pro Ser Thr Val Gly His Ala Lys Arg Glu Leu 
            180                 185                 190         


Leu Leu Arg Trp Leu Pro Glu Arg Leu Leu Thr Leu Asp Ser Leu Glu 
        195                 200                 205             


His Leu Pro Glu Arg Ile Leu His Asp Val Tyr Met His Cys Ser Tyr 
    210                 215                 220                 


Ala Asp Met Ala Glu Lys His Ala Ile Lys Arg Ser Ile Asn Phe His 
225                 230                 235                 240 


Leu Arg Asn Thr Leu Leu His Asn Gly Leu Ser Asp Asn His Leu Ser 
                245                 250                 255     


Pro Pro Ser Arg Asp Lys Pro Leu Met Leu Val Ile Leu Glu Trp Phe 
            260                 265                 270         


Asn Ser Gly His Ser Ile Tyr Arg Thr His Ser Ser Thr Leu Arg Ala 
        275                 280                 285             


Ala Arg Glu Gln Phe Ser Thr His Gly Ala Thr Ile Ile Asp Ala Thr 
    290                 295                 300                 


Asp Ala Ile Thr Gln Ala Val Phe Asp Asp Phe Thr Glu Val Asn Arg 
305                 310                 315                 320 


Ala Gly Ala Val Glu Ala Ile Val Ala Leu Thr Gln Gln Leu Leu Pro 
                325                 330                 335     


Asp Val Ile Tyr Phe Pro Ser Val Gly Met Phe Pro Leu Thr Ile Ala 
            340                 345                 350         


Leu Thr Asn Leu Arg Leu Ala Pro Leu Gln Val Met Ala Leu Gly His 
        355                 360                 365             


Pro Ala Thr Thr His Ser Asp Tyr Ile Asp Ala Val Leu Val Glu Glu 
    370                 375                 380                 


Asp Tyr Leu Gly Asp Ile Ala Cys Phe Ser Glu Lys Val Val Ser Leu 
385                 390                 395                 400 


Pro Lys Asp Cys Leu Pro Tyr Val Pro Pro Ala Asn Ile Ser Gln Pro 
                405                 410                 415     


Glu Pro Ile Leu His Phe Ala Glu Arg Pro Ala Val His Ile Ala Val 
            420                 425                 430         


Cys Ala Ser Ala Met Lys Ile Asn Pro Arg Phe Leu Ala Thr Cys Ala 
        435                 440                 445             


Glu Ile Thr Arg Gln Thr Ser Thr Ser Val Val Phe His Phe Leu Val 
    450                 455                 460                 


Gly Ala Cys Trp Gly Ile Thr His Arg Val Met Glu Lys Ala Val Asn 
465                 470                 475                 480 


Asp Ile Leu Pro Gln Ala Arg Val Tyr Glu His Leu Gly Tyr Leu Asp 
                485                 490                 495     


Tyr Leu Gln Val Ile Asn Gln Cys Asp Leu Phe Ile Asn Pro Phe Pro 
            500                 505                 510         


Phe Gly Asn Thr Asn Gly Ile Val Asp Thr Val Arg Gln Gly Leu Pro 
        515                 520                 525             


Gly Val Cys Leu Ser Gly Thr Glu Val His Glu His Ile Asp Glu Gly 
    530                 535                 540                 


Leu Phe Arg Arg Leu Gly Leu Asp Glu Glu Leu Ile Ala His Asp Leu 
545                 550                 555                 560 


Ala Glu Tyr Ile Ala Val Thr Val Arg Leu Ile Ser Asp Lys Glu Trp 
                565                 570                 575     


Arg Gln Ser Leu Arg Gln Arg Leu Leu Gln Ile Gln Pro Asp Asn Val 
            580                 585                 590         


Leu Phe Ala Gly Lys Pro Glu Gln Phe Gly Leu Ile Val Arg Gly Leu 
        595                 600                 605             


Leu Ala Asp Lys Lys Ala Ser Asp Lys Gly Gly 
    610                 615                 


<210>  17
<211>  617
<212>  PRT
<213>  Yersinia pestis

<400>  17

Met Ala Asp Lys Ser Val Glu Leu Thr Pro Val Val Glu Ala Pro Val 
1               5                   10                  15      


Val Phe Ser Leu Pro Tyr Phe Glu Phe Leu Val Cys Thr Arg Arg Tyr 
            20                  25                  30          


Glu Asp Ala Gly Arg Leu Leu Ile Leu Met Leu Glu Lys Leu Asp Thr 
        35                  40                  45              


Gln Tyr Gly Arg Trp Asp Val Phe Ser Leu Asn Lys Gln Pro Ile Gln 
    50                  55                  60                  


Gln Gln Glu Tyr Tyr Cys Asn Arg Leu Ala Ala Ala Ile Gly Cys Leu 
65                  70                  75                  80  


Phe Ser Asp Pro Gly Phe Val Ile Ser Glu Thr Gly Phe Leu Gln Leu 
                85                  90                  95      


Ile Asn Phe His Arg Trp Ile Ala Leu Ile Phe Ala Ala Ser Thr Phe 
            100                 105                 110         


Gly His Ala Asp His Val Ile Thr Asn Leu Asn Glu Ala Gly Asn Gly 
        115                 120                 125             


Cys Ser His Pro Leu Arg Phe Glu Arg Asn Asn Phe Leu Lys Phe Cys 
    130                 135                 140                 


Val Met Tyr Leu Pro Glu Ser Gly Ile Pro Leu Gln Pro Asp Ile Leu 
145                 150                 155                 160 


Trp Gln Phe Asn Pro Gln Ala Thr Ala Ala Leu Phe Leu Ala Leu Leu 
                165                 170                 175     


Ser Pro Arg Ile Leu Pro Ser Ala Ala Gly His Glu Lys Arg Glu Thr 
            180                 185                 190         


Leu Leu Ala Trp Leu Pro Glu Lys Leu Leu Thr Leu Ile Ser Leu Glu 
        195                 200                 205             


Gly Leu Pro Glu Arg Ile Leu His Asp Val Tyr Met His Cys Ser Tyr 
    210                 215                 220                 


Ala Asp Met Ala Lys Lys His Thr Ile Lys Arg Ser Ile Asn Phe His 
225                 230                 235                 240 


Leu Arg Lys Thr Met Leu Lys Asn Gly Leu Ser Asp Met Asn Glu Leu 
                245                 250                 255     


Pro Pro Leu Arg Ser Lys Pro Leu Met Leu Val Ile Leu Glu Trp Phe 
            260                 265                 270         


Asn Ser Gly His Ser Ile Tyr Arg Thr His Ser Ser Thr Leu Arg Ala 
        275                 280                 285             


Ala Arg Asp Gln Phe Ser Thr His Gly Val Ala Ile Ala Glu Ala Thr 
    290                 295                 300                 


Asp Asp Ile Thr Arg Lys Val Phe Asp Asp Phe Thr Glu Val Ser Arg 
305                 310                 315                 320 


Thr Gly Ala Val Glu Thr Ile Met Ala Leu Ala Gln Gln Leu Arg Pro 
                325                 330                 335     


Asp Val Ile Tyr Phe Pro Ser Val Gly Met Phe Pro Met Thr Val Ala 
            340                 345                 350         


Leu Thr Asn Leu Arg Leu Ala Pro Leu Gln Val Met Ala Leu Gly His 
        355                 360                 365             


Pro Ala Thr Thr His Ser Asp Tyr Ile Asp Ala Val Leu Val Glu Glu 
    370                 375                 380                 


Asp Tyr Leu Gly Asp Ile Ala Cys Phe Ser Glu Lys Val Val Ser Leu 
385                 390                 395                 400 


Pro Lys Asp Cys Leu Pro Tyr Val Pro Pro Ala Asn Ile Thr Gln Pro 
                405                 410                 415     


Glu Pro Ile Gln Gln Phe Val Gln Arg Glu Ala Val His Ile Ala Val 
            420                 425                 430         


Cys Ala Ser Ala Met Lys Ile Asn Pro Arg Phe Leu Ala Ala Cys Ala 
        435                 440                 445             


Glu Ile Ala Leu Arg Ser Pro Leu Pro Ile Ile Phe His Phe Leu Val 
    450                 455                 460                 


Gly Phe Cys Trp Gly Ile Thr His Arg Val Met Glu Lys Ala Val Asn 
465                 470                 475                 480 


Glu Met Val Thr Ser Ala Lys Val Tyr Glu His Leu Asn Tyr Gln Asn 
                485                 490                 495     


Tyr Leu Gln Val Ile Asn Gln Cys Asp Leu Phe Ile Asn Pro Phe Pro 
            500                 505                 510         


Phe Gly Asn Thr Asn Gly Ile Val Asp Thr Val Arg Gln Gly Leu Pro 
        515                 520                 525             


Gly Val Cys Leu Ser Gly Glu Glu Val His Glu His Ile Asp Glu Gly 
    530                 535                 540                 


Leu Phe Arg Arg Leu Gly Leu Ala Glu Glu Leu Ile Thr His Asn Val 
545                 550                 555                 560 


Glu Gln Tyr Ile Thr Ala Thr Val Arg Leu Ile Thr Asp Thr Asn Trp 
                565                 570                 575     


Arg Asn Gly Leu Arg Arg Gln Leu Leu Gln Thr Gln Pro Asp Asn Val 
            580                 585                 590         


Leu Phe Thr Gly Lys Pro Glu Gln Phe Gly Gln Ile Val Arg Ala Leu 
        595                 600                 605             


Leu Asp Asn Gly His Gln Asp Val Asn 
    610                 615         


<210>  18
<211>  617
<212>  PRT
<213>  Artificial

<220>
<223>  Modified Yersinia pestis NGT

<400>  18

Met Ala Asp Lys Ser Val Glu Leu Thr Pro Val Val Glu Ala Pro Val 
1               5                   10                  15      


Val Phe Ser Leu Pro Tyr Phe Glu Phe Leu Val Cys Thr Arg Arg Tyr 
            20                  25                  30          


Glu Asp Ala Gly Arg Leu Leu Ile Leu Met Leu Glu Lys Leu Asp Thr 
        35                  40                  45              


Gln Tyr Gly Arg Trp Asp Val Phe Ser Leu Asn Lys Gln Pro Ile Gln 
    50                  55                  60                  


Gln Gln Glu Tyr Tyr Cys Asn Arg Leu Ala Ala Ala Ile Gly Cys Leu 
65                  70                  75                  80  


Phe Ser Asp Pro Gly Phe Val Ile Ser Glu Thr Gly Phe Leu Gln Leu 
                85                  90                  95      


Ile Asn Phe His Arg Trp Ile Ala Leu Ile Phe Ala Ala Ser Thr Phe 
            100                 105                 110         


Gly His Ala Asp His Val Ile Thr Asn Leu Asn Glu Ala Gly Asn Gly 
        115                 120                 125             


Cys Ser His Pro Leu Arg Phe Glu Arg Asn Asn Phe Leu Lys Phe Cys 
    130                 135                 140                 


Val Met Tyr Leu Pro Glu Ser Gly Ile Pro Leu Gln Pro Asp Ile Leu 
145                 150                 155                 160 


Trp Gln Phe Asn Pro Gln Ala Thr Ala Ala Leu Phe Leu Ala Leu Leu 
                165                 170                 175     


Ser Pro Arg Ile Leu Pro Ser Ala Ala Gly His Glu Lys Arg Glu Thr 
            180                 185                 190         


Leu Leu Ala Trp Leu Pro Glu Lys Leu Leu Thr Leu Ile Ser Leu Glu 
        195                 200                 205             


Gly Leu Pro Glu Arg Ile Leu His Asp Val Tyr Met His Cys Ser Tyr 
    210                 215                 220                 


Ala Asp Met Ala Lys Lys His Thr Ile Lys Arg Ser Ile Asn Phe His 
225                 230                 235                 240 


Leu Arg Lys Thr Met Leu Lys Asn Gly Leu Ser Asp Met Asn Glu Leu 
                245                 250                 255     


Pro Pro Leu Arg Ser Lys Pro Leu Met Leu Val Ile Leu Glu Trp Phe 
            260                 265                 270         


Asn Ser Gly His Ser Ile Tyr Arg Thr His Ser Ser Thr Leu Arg Ala 
        275                 280                 285             


Ala Arg Asp Gln Phe Ser Thr His Gly Val Ala Ile Ala Glu Ala Thr 
    290                 295                 300                 


Asp Asp Ile Thr Arg Lys Val Phe Asp Asp Phe Thr Glu Val Ser Arg 
305                 310                 315                 320 


Thr Gly Ala Val Glu Thr Ile Met Ala Leu Ala Gln Gln Leu Arg Pro 
                325                 330                 335     


Asp Val Ile Tyr Phe Pro Ser Val Gly Met Phe Pro Met Thr Val Ala 
            340                 345                 350         


Leu Thr Asn Leu Arg Leu Ala Pro Leu Gln Val Met Ala Leu Gly His 
        355                 360                 365             


Pro Ala Thr Thr His Ser Asp Tyr Ile Asp Ala Val Leu Val Glu Glu 
    370                 375                 380                 


Asp Tyr Leu Gly Asp Ile Ala Cys Phe Ser Glu Lys Val Val Ser Leu 
385                 390                 395                 400 


Pro Lys Asp Cys Leu Pro Tyr Val Pro Pro Ala Asn Ile Thr Gln Pro 
                405                 410                 415     


Glu Pro Ile Gln Gln Phe Val Gln Arg Glu Ala Val His Ile Ala Val 
            420                 425                 430         


Cys Ala Ser Ala Met Lys Ile Asn Pro Arg Phe Leu Ala Ala Cys Ala 
        435                 440                 445             


Glu Ile Ala Leu Arg Ser Pro Leu Pro Ile Ile Phe His Phe Leu Val 
    450                 455                 460                 


Gly Ala Cys Trp Gly Ile Thr His Arg Val Met Glu Lys Ala Val Asn 
465                 470                 475                 480 


Glu Met Val Thr Ser Ala Lys Val Tyr Glu His Leu Asn Tyr Gln Asn 
                485                 490                 495     


Tyr Leu Gln Val Ile Asn Gln Cys Asp Leu Phe Ile Asn Pro Phe Pro 
            500                 505                 510         


Phe Gly Asn Thr Asn Gly Ile Val Asp Thr Val Arg Gln Gly Leu Pro 
        515                 520                 525             


Gly Val Cys Leu Ser Gly Glu Glu Val His Glu His Ile Asp Glu Gly 
    530                 535                 540                 


Leu Phe Arg Arg Leu Gly Leu Ala Glu Glu Leu Ile Thr His Asn Val 
545                 550                 555                 560 


Glu Gln Tyr Ile Thr Ala Thr Val Arg Leu Ile Thr Asp Thr Asn Trp 
                565                 570                 575     


Arg Asn Gly Leu Arg Arg Gln Leu Leu Gln Thr Gln Pro Asp Asn Val 
            580                 585                 590         


Leu Phe Thr Gly Lys Pro Glu Gln Phe Gly Gln Ile Val Arg Ala Leu 
        595                 600                 605             


Leu Asp Asn Gly His Gln Asp Val Asn 
    610                 615         


<210>  19
<211>  684
<212>  PRT
<213>  Kingella kingae

<400>  19

Met Thr Gln Thr Thr Glu Gln Ser Ile Pro Ser Leu Thr Arg Phe Glu 
1               5                   10                  15      


Gln Ala Val Ser Ser Gln Asn Tyr Glu Ala Ala Cys Thr Glu Leu Leu 
            20                  25                  30          


Ser Ile Leu Gly Gln Leu Asp Ser Asn Phe Gly Glu Ile His Gly Ile 
        35                  40                  45              


Glu Phe Ala Tyr Pro Val Gln Leu Gln Asn Leu Gln Gln Asp Val Thr 
    50                  55                  60                  


Ile His Phe Cys Thr Arg Met Ala Thr Ala Ile Thr Thr Leu Phe Thr 
65                  70                  75                  80  


Asn Lys Met Trp Ser Leu Thr Asp Asp Gly Arg Thr Arg Phe Leu Thr 
                85                  90                  95      


Val Gln Arg Trp Ile Asn Met Ile Phe Ala Ser Ser Pro Tyr Val Asn 
            100                 105                 110         


Ala Asp His Val Leu Ala Thr Tyr Asn Thr Asn Pro Glu Pro Asp Ser 
        115                 120                 125             


Leu Trp Asn Asn Ile His Leu Asp Asn Asn Gln Ser Ala Phe Asn Lys 
    130                 135                 140                 


Phe Ala Val Met Tyr Leu Pro Glu Ser Asn Val Gln Val Asn Leu Asp 
145                 150                 155                 160 


Ser Leu Trp Ser Val Asn Pro Ser Leu Thr Ala Ser Leu Cys Phe Ala 
                165                 170                 175     


Trp Gln Ser Pro Arg Phe Ile Ala Thr Glu Ala Ala Phe Asn Arg Arg 
            180                 185                 190         


Ala Gln Val Leu Gln Trp Phe Pro Ala Lys Leu Ala Gln Phe Asn Asn 
        195                 200                 205             


Leu Asn Thr Leu Pro Ala Asn Ile Ser His Asp Val Tyr Met His Cys 
    210                 215                 220                 


Ser Tyr Asp Ile Glu Pro Asn Lys His Asp Val Lys Gly Ala Leu Asn 
225                 230                 235                 240 


Gln Val Ile Arg Arg His Ile Leu Glu Glu Tyr Gly Trp Gln Asp Cys 
                245                 250                 255     


Asp Val Thr Lys Ile Gly Asn Ala His Gly Lys Pro Val Met Leu Val 
            260                 265                 270         


Leu Leu Glu His Phe His Ser Gly His Ser Ile Tyr Arg Thr His Ser 
        275                 280                 285             


Thr Ser Met Ile Ala Ala Arg Glu Gln Phe Tyr Leu Ile Gly Ile Gly 
    290                 295                 300                 


Gly Ala Ala Val Asp Glu Ala Gly Arg Ala Val Phe Asp Glu Phe Val 
305                 310                 315                 320 


Glu Ile Asp Ala Lys Ala Ser Thr Met Glu Lys Leu Gln Ala Ile Arg 
                325                 330                 335     


Ala Ile Ala Thr Lys Glu Gln Pro Ala Val Phe Tyr Met Pro Ser Ile 
            340                 345                 350         


Gly Met Asp Leu Ile Thr Ile Phe Ala Ser Asn Thr Arg Ile Ala Pro 
        355                 360                 365             


Ile Gln Val Ile Ala Leu Gly His Pro Ala Thr Thr His Ser Lys Phe 
    370                 375                 380                 


Ile Glu Tyr Val Ile Val Glu Asp Asp Tyr Val Gly Ser Glu Glu Cys 
385                 390                 395                 400 


Phe Ser Glu Thr Leu Leu Arg Leu Pro Lys Asp Ala Leu Pro Tyr Val 
                405                 410                 415     


Pro Ser Ala Leu Ala Pro Ala Ser Val Glu Tyr Asn Leu Arg Glu Asn 
            420                 425                 430         


Pro Ser Val Val His Ile Gly Val Ala Ser Thr Thr Met Lys Leu Asn 
        435                 440                 445             


Pro Tyr Phe Leu Arg Ala Cys Ala Glu Ile Lys Ala Arg Ser Lys Val 
    450                 455                 460                 


Pro Val His Phe His Phe Ala Met Gly Gln Ala Ser Gly Val Thr Phe 
465                 470                 475                 480 


Ala Tyr Ile Glu Arg Phe Leu Lys Thr Tyr Leu Gly Lys Ala Val Thr 
                485                 490                 495     


Ala Tyr Pro His Gln Ser Tyr Thr Asp Tyr Leu Arg Thr Leu His Gln 
            500                 505                 510         


Cys Asp Met Met Ile Asn Pro Phe Pro Phe Gly Asn Thr Asn Gly Ile 
        515                 520                 525             


Ile Asp Met Val Thr Leu Gly Leu Val Gly Ile Cys Lys Thr Gly Ala 
    530                 535                 540                 


Glu Val His Glu His Ile Asp Glu Gly Leu Phe Lys Arg Leu Gly Leu 
545                 550                 555                 560 


Pro Glu Trp Leu Ile Thr Gln Thr Ala Asp Asp Tyr Val Asn Cys Ala 
                565                 570                 575     


Val Arg Leu Ala Glu Asn His Glu Glu Arg Leu Ala Leu Arg Arg His 
            580                 585                 590         


Ile Ile Glu Asn Asn Gly Leu Asn Thr Leu Phe Ser Gly Asp Pro Lys 
        595                 600                 605             


Pro Met Gly Gln Ile Leu Trp Ala Lys Val Gln Glu Lys Met Ala Lys 
    610                 615                 620                 


Pro Ala Lys Lys Ala Thr Ala Lys Val Ala Ser Lys Pro Ala Thr Ala 
625                 630                 635                 640 


Val Glu Pro Val Ala Glu Lys Pro Ala Thr Lys Thr Val Arg Lys Thr 
                645                 650                 655     


Ala Ser Lys Lys Ala Ala Ala Thr Glu Ala Thr Thr Glu Lys Ala Ala 
            660                 665                 670         


Pro Lys Thr Thr Arg Thr Arg Lys Lys Ala Ala Glu 
        675                 680                 


<210>  20
<211>  684
<212>  PRT
<213>  Artificial

<220>
<223>  Modified Kingella kingae NGT

<400>  20

Met Thr Gln Thr Thr Glu Gln Ser Ile Pro Ser Leu Thr Arg Phe Glu 
1               5                   10                  15      


Gln Ala Val Ser Ser Gln Asn Tyr Glu Ala Ala Cys Thr Glu Leu Leu 
            20                  25                  30          


Ser Ile Leu Gly Gln Leu Asp Ser Asn Phe Gly Glu Ile His Gly Ile 
        35                  40                  45              


Glu Phe Ala Tyr Pro Val Gln Leu Gln Asn Leu Gln Gln Asp Val Thr 
    50                  55                  60                  


Ile His Phe Cys Thr Arg Met Ala Thr Ala Ile Thr Thr Leu Phe Thr 
65                  70                  75                  80  


Asn Lys Met Trp Ser Leu Thr Asp Asp Gly Arg Thr Arg Phe Leu Thr 
                85                  90                  95      


Val Gln Arg Trp Ile Asn Met Ile Phe Ala Ser Ser Pro Tyr Val Asn 
            100                 105                 110         


Ala Asp His Val Leu Ala Thr Tyr Asn Thr Asn Pro Glu Pro Asp Ser 
        115                 120                 125             


Leu Trp Asn Asn Ile His Leu Asp Asn Asn Gln Ser Ala Phe Asn Lys 
    130                 135                 140                 


Phe Ala Val Met Tyr Leu Pro Glu Ser Asn Val Gln Val Asn Leu Asp 
145                 150                 155                 160 


Ser Leu Trp Ser Val Asn Pro Ser Leu Thr Ala Ser Leu Cys Phe Ala 
                165                 170                 175     


Trp Gln Ser Pro Arg Phe Ile Ala Thr Glu Ala Ala Phe Asn Arg Arg 
            180                 185                 190         


Ala Gln Val Leu Gln Trp Phe Pro Ala Lys Leu Ala Gln Phe Asn Asn 
        195                 200                 205             


Leu Asn Thr Leu Pro Ala Asn Ile Ser His Asp Val Tyr Met His Cys 
    210                 215                 220                 


Ser Tyr Asp Ile Glu Pro Asn Lys His Asp Val Lys Gly Ala Leu Asn 
225                 230                 235                 240 


Gln Val Ile Arg Arg His Ile Leu Glu Glu Tyr Gly Trp Gln Asp Cys 
                245                 250                 255     


Asp Val Thr Lys Ile Gly Asn Ala His Gly Lys Pro Val Met Leu Val 
            260                 265                 270         


Leu Leu Glu His Phe His Ser Gly His Ser Ile Tyr Arg Thr His Ser 
        275                 280                 285             


Thr Ser Met Ile Ala Ala Arg Glu Gln Phe Tyr Leu Ile Gly Ile Gly 
    290                 295                 300                 


Gly Ala Ala Val Asp Glu Ala Gly Arg Ala Val Phe Asp Glu Phe Val 
305                 310                 315                 320 


Glu Ile Asp Ala Lys Ala Ser Thr Met Glu Lys Leu Gln Ala Ile Arg 
                325                 330                 335     


Ala Ile Ala Thr Lys Glu Gln Pro Ala Val Phe Tyr Met Pro Ser Ile 
            340                 345                 350         


Gly Met Asp Leu Ile Thr Ile Phe Ala Ser Asn Thr Arg Ile Ala Pro 
        355                 360                 365             


Ile Gln Val Ile Ala Leu Gly His Pro Ala Thr Thr His Ser Lys Phe 
    370                 375                 380                 


Ile Glu Tyr Val Ile Val Glu Asp Asp Tyr Val Gly Ser Glu Glu Cys 
385                 390                 395                 400 


Phe Ser Glu Thr Leu Leu Arg Leu Pro Lys Asp Ala Leu Pro Tyr Val 
                405                 410                 415     


Pro Ser Ala Leu Ala Pro Ala Ser Val Glu Tyr Asn Leu Arg Glu Asn 
            420                 425                 430         


Pro Ser Val Val His Ile Gly Val Ala Ser Thr Thr Met Lys Leu Asn 
        435                 440                 445             


Pro Tyr Phe Leu Arg Ala Cys Ala Glu Ile Lys Ala Arg Ser Lys Val 
    450                 455                 460                 


Pro Val His Phe His Phe Ala Met Gly Ala Ala Ser Gly Val Thr Phe 
465                 470                 475                 480 


Ala Tyr Ile Glu Arg Phe Leu Lys Thr Tyr Leu Gly Lys Ala Val Thr 
                485                 490                 495     


Ala Tyr Pro His Gln Ser Tyr Thr Asp Tyr Leu Arg Thr Leu His Gln 
            500                 505                 510         


Cys Asp Met Met Ile Asn Pro Phe Pro Phe Gly Asn Thr Asn Gly Ile 
        515                 520                 525             


Ile Asp Met Val Thr Leu Gly Leu Val Gly Ile Cys Lys Thr Gly Ala 
    530                 535                 540                 


Glu Val His Glu His Ile Asp Glu Gly Leu Phe Lys Arg Leu Gly Leu 
545                 550                 555                 560 


Pro Glu Trp Leu Ile Thr Gln Thr Ala Asp Asp Tyr Val Asn Cys Ala 
                565                 570                 575     


Val Arg Leu Ala Glu Asn His Glu Glu Arg Leu Ala Leu Arg Arg His 
            580                 585                 590         


Ile Ile Glu Asn Asn Gly Leu Asn Thr Leu Phe Ser Gly Asp Pro Lys 
        595                 600                 605             


Pro Met Gly Gln Ile Leu Trp Ala Lys Val Gln Glu Lys Met Ala Lys 
    610                 615                 620                 


Pro Ala Lys Lys Ala Thr Ala Lys Val Ala Ser Lys Pro Ala Thr Ala 
625                 630                 635                 640 


Val Glu Pro Val Ala Glu Lys Pro Ala Thr Lys Thr Val Arg Lys Thr 
                645                 650                 655     


Ala Ser Lys Lys Ala Ala Ala Thr Glu Ala Thr Thr Glu Lys Ala Ala 
            660                 665                 670         


Pro Lys Thr Thr Arg Thr Arg Lys Lys Ala Ala Glu 
        675                 680                 


