
                                SEQUENCE LISTING

<110> Shipman, Seth
      The J. David Gladstone Institutes, a testamentary trust
      established under the Will of J. David Gladstone

<120> MODIFIED MITOCHONDRION AND METHODS OF USE THEREOF

<130> 3730.048WO1

<140> PCT/US2020/017462   
<141> 2020-02-10  

<150> US 62/803,546       
<151> 2019-02-10  

<160> 12

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 14
<212> PRT
<213> Artificial Sequence

<220> 
<223> A synthetic polypeptide

<400> 1
Lys Leu Ala Lys Leu Ala Lys Lys Leu Ala Lys Leu Ala Lys
 1               5                  10

<210> 2
<211> 7
<212> PRT
<213> Artificial Sequence

<220> 
<223> A synthetic polypeptide

<400> 2
Gly Asp Pro Pro Val Ala Thr
 1               5

<210> 3
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> A synthetic oligonucleotide

<400> 3
acgctcagga aatagaaacc gtc                                              23

<210> 4
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> A synthetic oligonucleotide

<400> 4
gtggtttgct ccacagattt cag                                              23

<210> 5
<211> 22
<212> DNA
<213> Artificial Sequence

<220> 
<223> A synthetic oligonucleotide

<400> 5
ctcccctgaa ctctacacaa ca                                               22

<210> 6
<211> 19
<212> DNA
<213> Artificial Sequence

<220> 
<223> A synthetic oligonucleotide

<400> 6
ggatgcggtt gcttgcgtg                                                   19

<210> 7
<211> 24
<212> DNA
<213> Artificial Sequence

<220> 
<223> A synthetic oligonucleotide

<400> 7
catccccttc tgtgaatgtt agac                                             24

<210> 8
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> A synthetic oligonucleotide

<400> 8
gattggagac acggagagca g                                                21

<210> 9
<211> 22
<212> DNA
<213> Artificial Sequence

<220> 
<223> A synthetic oligonucleotide

<400> 9
gagctggtct gttggagaag tc                                               22

<210> 10
<211> 24
<212> DNA
<213> Artificial Sequence

<220> 
<223> A synthetic oligonucleotide

<400> 10
caatccataa tcccacgttg catg                                             24

<210> 11
<211> 649
<212> PRT
<213> Artificial Sequence

<220> 
<223> A synthetic polypeptide

<400> 11
Met Val Gly Arg Asn Ser Ala Ile Ala Ala Gly Val Cys Gly Ala Leu
 1               5                  10                  15
Phe Ile Gly Tyr Cys Ile Tyr Phe Asp Arg Lys Arg Arg Ser Asp Pro
            20                  25                  30
Asn Phe Lys Asn Arg Leu Arg Glu Arg Arg Lys Lys Gln Lys Leu Ala
        35                  40                  45
Lys Glu Arg Ala Gly Leu Ser Lys Leu Pro Asp Leu Lys Asp Ala Glu
    50                  55                  60
Ala Val Gln Lys Phe Phe Leu Glu Glu Ile Gln Leu Gly Glu Glu Leu
65                  70                  75                  80
Leu Ala Gln Gly Glu Tyr Glu Lys Gly Val Asp His Leu Thr Asn Ala
                85                  90                  95
Ile Ala Val Cys Gly Gln Pro Gln Gln Leu Leu Gln Val Leu Gln Gln
            100                 105                 110
Thr Leu Pro Pro Pro Val Phe Gln Met Leu Leu Thr Lys Leu Pro Thr
        115                 120                 125
Ile Ser Gln Arg Ile Val Ser Ala Gln Ser Leu Ala Glu Asp Asp Val
    130                 135                 140
Glu Gly Asp Pro Pro Val Ala Thr Asn Ser Phe Thr Leu Ser Val Thr
145                 150                 155                 160
Val Gln Gln Pro Gln Leu Thr Leu Thr Ala Ala Val Ile Gly Asp Gly
                165                 170                 175
Ala Pro Ala Asn Gly Lys Thr Ala Ile Thr Val Glu Phe Thr Val Ala
            180                 185                 190
Asp Phe Glu Gly Lys Pro Leu Ala Gly Gln Glu Val Val Ile Thr Thr
        195                 200                 205
Asn Asn Gly Ala Leu Pro Asn Lys Ile Thr Glu Lys Thr Asp Ala Asn
    210                 215                 220
Gly Val Ala Arg Ile Ala Leu Thr Asn Thr Thr Asp Gly Val Thr Val
225                 230                 235                 240
Val Thr Ala Glu Val Glu Gly Gln Arg Gln Ser Val Asp Thr His Phe
                245                 250                 255
Val Lys Gly Thr Ile Thr Ala Asp Lys Ser Thr Leu Thr Ala Val Pro
            260                 265                 270
Thr Ser Ile Ile Ala Asp Gly Leu Met Ala Ser Thr Ile Thr Leu Glu
        275                 280                 285
Leu Lys Asp Thr Tyr Gly Asp Pro Gln Ala Gly Ala Asn Val Ala Phe
    290                 295                 300
Asp Thr Thr Leu Gly Asn Met Gly Val Ile Thr Asp His Asn Asp Gly
305                 310                 315                 320
Thr Tyr Ser Ala Pro Leu Thr Ser Thr Thr Leu Gly Val Ala Thr Val
                325                 330                 335
Thr Val Lys Val Asp Gly Ala Ala Phe Ser Val Pro Ser Val Thr Val
            340                 345                 350
Asn Phe Thr Ala Asp Pro Ile Pro Asp Ala Gly Arg Ser Ser Phe Thr
        355                 360                 365
Val Ser Thr Pro Asp Ile Leu Ala Asp Gly Thr Met Ser Ser Thr Leu
    370                 375                 380
Ser Phe Val Pro Val Asp Lys Asn Gly His Phe Ile Ser Gly Met Gln
385                 390                 395                 400
Gly Leu Ser Phe Thr Gln Asn Gly Val Pro Val Ser Ile Ser Pro Ile
                405                 410                 415
Thr Glu Gln Pro Asp Ser Tyr Thr Ala Thr Val Val Gly Asn Thr Ala
            420                 425                 430
Gly Asp Val Thr Ile Thr Pro Gln Val Asp Thr Leu Ile Leu Ser Thr
        435                 440                 445
Leu Gln Lys Lys Ile Ser Leu Phe Pro Val Pro Thr Leu Thr Gly Ile
    450                 455                 460
Leu Val Asn Gly Gln Asn Phe Ala Thr Asp Lys Gly Phe Pro Lys Thr
465                 470                 475                 480
Ile Phe Lys Asn Ala Thr Phe Gln Leu Gln Met Asp Asn Asp Val Ala
                485                 490                 495
Asn Asn Thr Gln Tyr Glu Trp Ser Ser Ser Phe Thr Pro Asn Val Ser
            500                 505                 510
Val Asn Asp Gln Gly Gln Val Thr Ile Thr Tyr Gln Thr Tyr Ser Glu
        515                 520                 525
Val Ala Val Thr Ala Lys Ser Lys Lys Phe Pro Ser Tyr Ser Val Ser
    530                 535                 540
Tyr Arg Phe Tyr Pro Asn Arg Trp Ile Tyr Asp Gly Gly Thr Ser Leu
545                 550                 555                 560
Val Ser Ser Leu Glu Ala Ser Arg Gln Cys Gln Gly Ser Asp Met Ser
                565                 570                 575
Ala Val Leu Glu Ser Ser Arg Ala Thr Asn Gly Thr Arg Ala Pro Asp
            580                 585                 590
Gly Thr Leu Trp Gly Glu Trp Gly Ser Leu Thr Ala Tyr Ser Ser Asp
        595                 600                 605
Trp Gln Ser Gly Glu Tyr Trp Val Lys Lys Thr Ser Thr Asp Phe Glu
    610                 615                 620
Thr Met Asn Met Asp Thr Gly Ala Leu Val Gln Gly Pro Ala Tyr Leu
625                 630                 635                 640
Ala Phe Pro Leu Cys Ala Leu Ala Ile
                645

<210> 12
<211> 537
<212> PRT
<213> Artificial Sequence

<220> 
<223> A synthetic polypeptide

<400> 12
Met Val Gly Arg Asn Ser Ala Ile Ala Ala Gly Val Cys Gly Ala Leu
 1               5                  10                  15
Phe Ile Gly Tyr Cys Ile Tyr Phe Asp Arg Lys Arg Arg Ser Asp Pro
            20                  25                  30
Asn Gly Asp Pro Pro Val Ala Thr Asn Ser Phe Thr Leu Ser Val Thr
        35                  40                  45
Val Gln Gln Pro Gln Leu Thr Leu Thr Ala Ala Val Ile Gly Asp Gly
    50                  55                  60
Ala Pro Ala Asn Gly Lys Thr Ala Ile Thr Val Glu Phe Thr Val Ala
65                  70                  75                  80
Asp Phe Glu Gly Lys Pro Leu Ala Gly Gln Glu Val Val Ile Thr Thr
                85                  90                  95
Asn Asn Gly Ala Leu Pro Asn Lys Ile Thr Glu Lys Thr Asp Ala Asn
            100                 105                 110
Gly Val Ala Arg Ile Ala Leu Thr Asn Thr Thr Asp Gly Val Thr Val
        115                 120                 125
Val Thr Ala Glu Val Glu Gly Gln Arg Gln Ser Val Asp Thr His Phe
    130                 135                 140
Val Lys Gly Thr Ile Thr Ala Asp Lys Ser Thr Leu Thr Ala Val Pro
145                 150                 155                 160
Thr Ser Ile Ile Ala Asp Gly Leu Met Ala Ser Thr Ile Thr Leu Glu
                165                 170                 175
Leu Lys Asp Thr Tyr Gly Asp Pro Gln Ala Gly Ala Asn Val Ala Phe
            180                 185                 190
Asp Thr Thr Leu Gly Asn Met Gly Val Ile Thr Asp His Asn Asp Gly
        195                 200                 205
Thr Tyr Ser Ala Pro Leu Thr Ser Thr Thr Leu Gly Val Ala Thr Val
    210                 215                 220
Thr Val Lys Val Asp Gly Ala Ala Phe Ser Val Pro Ser Val Thr Val
225                 230                 235                 240
Asn Phe Thr Ala Asp Pro Ile Pro Asp Ala Gly Arg Ser Ser Phe Thr
                245                 250                 255
Val Ser Thr Pro Asp Ile Leu Ala Asp Gly Thr Met Ser Ser Thr Leu
            260                 265                 270
Ser Phe Val Pro Val Asp Lys Asn Gly His Phe Ile Ser Gly Met Gln
        275                 280                 285
Gly Leu Ser Phe Thr Gln Asn Gly Val Pro Val Ser Ile Ser Pro Ile
    290                 295                 300
Thr Glu Gln Pro Asp Ser Tyr Thr Ala Thr Val Val Gly Asn Thr Ala
305                 310                 315                 320
Gly Asp Val Thr Ile Thr Pro Gln Val Asp Thr Leu Ile Leu Ser Thr
                325                 330                 335
Leu Gln Lys Lys Ile Ser Leu Phe Pro Val Pro Thr Leu Thr Gly Ile
            340                 345                 350
Leu Val Asn Gly Gln Asn Phe Ala Thr Asp Lys Gly Phe Pro Lys Thr
        355                 360                 365
Ile Phe Lys Asn Ala Thr Phe Gln Leu Gln Met Asp Asn Asp Val Ala
    370                 375                 380
Asn Asn Thr Gln Tyr Glu Trp Ser Ser Ser Phe Thr Pro Asn Val Ser
385                 390                 395                 400
Val Asn Asp Gln Gly Gln Val Thr Ile Thr Tyr Gln Thr Tyr Ser Glu
                405                 410                 415
Val Ala Val Thr Ala Lys Ser Lys Lys Phe Pro Ser Tyr Ser Val Ser
            420                 425                 430
Tyr Arg Phe Tyr Pro Asn Arg Trp Ile Tyr Asp Gly Gly Thr Ser Leu
        435                 440                 445
Val Ser Ser Leu Glu Ala Ser Arg Gln Cys Gln Gly Ser Asp Met Ser
    450                 455                 460
Ala Val Leu Glu Ser Ser Arg Ala Thr Asn Gly Thr Arg Ala Pro Asp
465                 470                 475                 480
Gly Thr Leu Trp Gly Glu Trp Gly Ser Leu Thr Ala Tyr Ser Ser Asp
                485                 490                 495
Trp Gln Ser Gly Glu Tyr Trp Val Lys Lys Thr Ser Thr Asp Phe Glu
            500                 505                 510
Thr Met Asn Met Asp Thr Gly Ala Leu Val Gln Gly Pro Ala Tyr Leu
        515                 520                 525
Ala Phe Pro Leu Cys Ala Leu Ala Ile
    530                 535
