                               SEQUENCE LISTING

<110> THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
 
<120> EXERCISE-INDUCED CIRCULATORY FACTORS FOR AMELIORATION OF 
      COGNITIVE, NEUROLOGICAL, AND REGENERATIVE DYSFUNCTION DURING 
      AGING

<130> 081906-1175384-233110WO

<140> PCT/US2020/016549
<141> 2020-02-04

<150> 62/800,944
<151> 2019-02-04

<160> 15    

<170> PatentIn version 3.5

<210> 1
<211> 840
<212> PRT
<213> Homo sapiens

<400> 1
Met Ser Ala Phe Arg Leu Trp Pro Gly Leu Leu Ile Met Leu Gly Ser 
1               5                   10                  15      


Leu Cys His Arg Gly Ser Pro Cys Gly Leu Ser Thr His Val Glu Ile 
            20                  25                  30          


Gly His Arg Ala Leu Glu Phe Leu Gln Leu His Asn Gly Arg Val Asn 
        35                  40                  45              


Tyr Arg Glu Leu Leu Leu Glu His Gln Asp Ala Tyr Gln Ala Gly Ile 
    50                  55                  60                  


Val Phe Pro Asp Cys Phe Tyr Pro Ser Ile Cys Lys Gly Gly Lys Phe 
65                  70                  75                  80  


His Asp Val Ser Glu Ser Thr His Trp Thr Pro Phe Leu Asn Ala Ser 
                85                  90                  95      


Val His Tyr Ile Arg Glu Asn Tyr Pro Leu Pro Trp Glu Lys Asp Thr 
            100                 105                 110         


Glu Lys Leu Val Ala Phe Leu Phe Gly Ile Thr Ser His Met Ala Ala 
        115                 120                 125             


Asp Val Ser Trp His Ser Leu Gly Leu Glu Gln Gly Phe Leu Arg Thr 
    130                 135                 140                 


Met Gly Ala Ile Asp Phe His Gly Ser Tyr Ser Glu Ala His Ser Ala 
145                 150                 155                 160 


Gly Asp Phe Gly Gly Asp Val Leu Ser Gln Phe Glu Phe Asn Phe Asn 
                165                 170                 175     


Tyr Leu Ala Arg Arg Trp Tyr Val Pro Val Lys Asp Leu Leu Gly Ile 
            180                 185                 190         


Tyr Glu Lys Leu Tyr Gly Arg Lys Val Ile Thr Glu Asn Val Ile Val 
        195                 200                 205             


Asp Cys Ser His Ile Gln Phe Leu Glu Met Tyr Gly Glu Met Leu Ala 
    210                 215                 220                 


Val Ser Lys Leu Tyr Pro Thr Tyr Ser Thr Lys Ser Pro Phe Leu Val 
225                 230                 235                 240 


Glu Gln Phe Gln Glu Tyr Phe Leu Gly Gly Leu Asp Asp Met Ala Phe 
                245                 250                 255     


Trp Ser Thr Asn Ile Tyr His Leu Thr Ser Phe Met Leu Glu Asn Gly 
            260                 265                 270         


Thr Ser Asp Cys Asn Leu Pro Glu Asn Pro Leu Phe Ile Ala Cys Gly 
        275                 280                 285             


Gly Gln Gln Asn His Thr Gln Gly Ser Lys Met Gln Lys Asn Asp Phe 
    290                 295                 300                 


His Arg Asn Leu Thr Thr Ser Leu Thr Glu Ser Val Asp Arg Asn Ile 
305                 310                 315                 320 


Asn Tyr Thr Glu Arg Gly Val Phe Phe Ser Val Asn Ser Trp Thr Pro 
                325                 330                 335     


Asp Ser Met Ser Phe Ile Tyr Lys Ala Leu Glu Arg Asn Ile Arg Thr 
            340                 345                 350         


Met Phe Ile Gly Gly Ser Gln Leu Ser Gln Lys His Val Ser Ser Pro 
        355                 360                 365             


Leu Ala Ser Tyr Phe Leu Ser Phe Pro Tyr Ala Arg Leu Gly Trp Ala 
    370                 375                 380                 


Met Thr Ser Ala Asp Leu Asn Gln Asp Gly His Gly Asp Leu Val Val 
385                 390                 395                 400 


Gly Ala Pro Gly Tyr Ser Arg Pro Gly His Ile His Ile Gly Arg Val 
                405                 410                 415     


Tyr Leu Ile Tyr Gly Asn Asp Leu Gly Leu Pro Pro Val Asp Leu Asp 
            420                 425                 430         


Leu Asp Lys Glu Ala His Arg Ile Leu Glu Gly Phe Gln Pro Ser Gly 
        435                 440                 445             


Arg Phe Gly Ser Ala Leu Ala Val Leu Asp Phe Asn Val Asp Gly Val 
    450                 455                 460                 


Pro Asp Leu Ala Val Gly Ala Pro Ser Val Gly Ser Glu Gln Leu Thr 
465                 470                 475                 480 


Tyr Lys Gly Ala Val Tyr Val Tyr Phe Gly Ser Lys Gln Gly Gly Met 
                485                 490                 495     


Ser Ser Ser Pro Asn Ile Thr Ile Ser Cys Gln Asp Ile Tyr Cys Asn 
            500                 505                 510         


Leu Gly Trp Thr Leu Leu Ala Ala Asp Val Asn Gly Asp Ser Glu Pro 
        515                 520                 525             


Asp Leu Val Ile Gly Ser Pro Phe Ala Pro Gly Gly Gly Lys Gln Lys 
    530                 535                 540                 


Gly Ile Val Ala Ala Phe Tyr Ser Gly Pro Ser Leu Ser Asp Lys Glu 
545                 550                 555                 560 


Lys Leu Asn Val Glu Ala Ala Asn Trp Thr Val Arg Gly Glu Glu Asp 
                565                 570                 575     


Phe Ser Trp Phe Gly Tyr Ser Leu His Gly Val Thr Val Asp Asn Arg 
            580                 585                 590         


Thr Leu Leu Leu Val Gly Ser Pro Thr Trp Lys Asn Ala Ser Arg Leu 
        595                 600                 605             


Gly His Leu Leu His Ile Arg Asp Glu Lys Lys Ser Leu Gly Arg Val 
    610                 615                 620                 


Tyr Gly Tyr Phe Pro Pro Asn Gly Gln Ser Trp Phe Thr Ile Ser Gly 
625                 630                 635                 640 


Asp Lys Ala Met Gly Lys Leu Gly Thr Ser Leu Ser Ser Gly His Val 
                645                 650                 655     


Leu Met Asn Gly Thr Leu Lys Gln Val Leu Leu Val Gly Ala Pro Thr 
            660                 665                 670         


Tyr Asp Asp Val Ser Lys Val Ala Phe Leu Thr Val Thr Leu His Gln 
        675                 680                 685             


Gly Gly Ala Thr Arg Met Tyr Ala Leu Thr Ser Asp Ala Gln Pro Leu 
    690                 695                 700                 


Leu Leu Ser Thr Phe Ser Gly Asp Arg Arg Phe Ser Arg Phe Gly Gly 
705                 710                 715                 720 


Val Leu His Leu Ser Asp Leu Asp Asp Asp Gly Leu Asp Glu Ile Ile 
                725                 730                 735     


Met Ala Ala Pro Leu Arg Ile Ala Asp Val Thr Ser Gly Leu Ile Gly 
            740                 745                 750         


Gly Glu Asp Gly Arg Val Tyr Val Tyr Asn Gly Lys Glu Thr Thr Leu 
        755                 760                 765             


Gly Asp Met Thr Gly Lys Cys Lys Ser Trp Ile Thr Pro Cys Pro Glu 
    770                 775                 780                 


Glu Lys Ala Gln Tyr Val Leu Ile Ser Pro Glu Ala Ser Ser Arg Phe 
785                 790                 795                 800 


Gly Ser Ser Leu Ile Thr Val Arg Ser Lys Ala Lys Asn Gln Val Val 
                805                 810                 815     


Ile Ala Ala Gly Arg Ser Ser Leu Gly Ala Arg Leu Ser Gly Ala Leu 
            820                 825                 830         


His Val Tyr Ser Leu Gly Ser Asp 
        835                 840 


<210> 2
<211> 837
<212> PRT
<213> Mus musculus

<400> 2
Met Ser Ala Gly Arg Leu Trp Ser Ser Leu Leu Leu Leu Leu Pro Leu 
1               5                   10                  15      


Phe Cys Ser Lys Ser Ser Ser Cys Gly Leu Ser Thr His Val Glu Ile 
            20                  25                  30          


Gly His Arg Ala Leu Glu Phe Leu Arg Leu Gln Asp Gly Arg Ile Asn 
        35                  40                  45              


Tyr Lys Glu Leu Ile Leu Glu His Gln Asp Ala Tyr Gln Ala Gly Thr 
    50                  55                  60                  


Val Phe Pro Asp Ala Phe Tyr Pro Ser Ile Cys Lys Arg Gly Lys Tyr 
65                  70                  75                  80  


His Asp Val Ser Glu Arg Thr His Trp Thr Pro Phe Leu Asn Ala Ser 
                85                  90                  95      


Ile His Tyr Ile Arg Glu Asn Tyr Pro Leu Pro Trp Glu Lys Asp Thr 
            100                 105                 110         


Glu Lys Leu Val Ala Phe Leu Phe Gly Ile Thr Ser His Met Val Ala 
        115                 120                 125             


Asp Leu Ser Trp His Asn Leu Gly Phe Leu Arg Thr Met Gly Ala Ile 
    130                 135                 140                 


Asp Phe Tyr Asn Ser Tyr Ser Asp Ala His Ser Ala Gly Asp Phe Gly 
145                 150                 155                 160 


Gly Asp Val Leu Ser Gln Phe Glu Phe Asn Phe Asn Tyr Leu Ser Arg 
                165                 170                 175     


Arg Trp Tyr Val Pro Val Arg Asp Leu Leu Arg Ile Tyr Asp Asn Leu 
            180                 185                 190         


Tyr Gly Arg Lys Val Ile Thr Lys Asp Val Leu Val Asp Cys Thr Tyr 
        195                 200                 205             


Leu Gln Phe Leu Glu Met His Gly Glu Met Phe Ala Val Ser Lys Leu 
    210                 215                 220                 


Tyr Ser Thr Tyr Ser Thr Lys Ser Pro Phe Leu Val Glu Gln Phe Gln 
225                 230                 235                 240 


Asp Tyr Phe Leu Gly Gly Leu Asp Asp Met Ala Phe Trp Ser Thr Asn 
                245                 250                 255     


Ile Tyr Arg Leu Thr Ser Phe Met Leu Glu Asn Gly Thr Ser Asp Cys 
            260                 265                 270         


Asn Leu Pro Glu Asn Pro Leu Phe Ile Ser Cys Asp Gly Arg Asn His 
        275                 280                 285             


Thr Leu Ser Gly Ser Lys Val Gln Lys Asn Asp Phe His Arg Asn Leu 
    290                 295                 300                 


Thr Met Phe Ile Ser Arg Asp Ile Arg Lys Asn Leu Asn Tyr Thr Glu 
305                 310                 315                 320 


Arg Gly Val Phe Tyr Ser Thr Gly Ser Trp Ala Arg Pro Glu Ser Val 
                325                 330                 335     


Thr Phe Met Tyr Gln Thr Leu Glu Arg Asn Leu Arg Leu Met Leu Ala 
            340                 345                 350         


Gly Ser Ser Gln Lys Asn Leu Asn His Val Ser Ser Pro Ser Ala Ser 
        355                 360                 365             


Tyr Thr Leu Ser Val Pro Tyr Ala Arg Leu Gly Trp Val Met Thr Ser 
    370                 375                 380                 


Ala Asp Leu Asn Gln Asp Gly His Gly Asp Leu Val Val Gly Ala Pro 
385                 390                 395                 400 


Gly Tyr Ser His Pro Gly Arg Phe Gln Ile Gly Arg Val Tyr Ile Ile 
                405                 410                 415     


Tyr Gly Asn Asp Leu Gly Leu Pro Pro Ile Asp Leu Asp Leu Asn Lys 
            420                 425                 430         


Glu Gly Ile Leu Glu Gly Phe Gln Pro Ser Gly Arg Phe Gly Ser Ala 
        435                 440                 445             


Leu Ala Val Leu Asp Phe Asn Gln Asp Gly Leu Pro Asp Leu Ala Val 
    450                 455                 460                 


Gly Ala Pro Ser Val Gly Ser Gly Gln Leu Thr Tyr Asn Gly Ser Val 
465                 470                 475                 480 


Tyr Val Tyr Tyr Gly Ser Gln Gln Gly Arg Leu Ser Ser Ser Pro Asn 
                485                 490                 495     


Val Thr Ile Ser Cys Lys Asp Thr Tyr Cys Asn Leu Gly Trp Thr Leu 
            500                 505                 510         


Leu Ala Thr Asp Ala Asp Gly Asp Gly Arg His Asp Leu Val Ile Ser 
        515                 520                 525             


Ser Pro Phe Ala Pro Gly Gly Arg Lys Gln Lys Gly Ile Val Ala Thr 
    530                 535                 540                 


Phe Tyr Ser His Pro Arg Arg Asn Asp Lys Glu Leu Leu Thr Leu Glu 
545                 550                 555                 560 


Glu Ala Asp Trp Lys Val Asn Gly Glu Glu Asp Phe Ser Trp Phe Gly 
                565                 570                 575     


Tyr Ser Leu His Gly Val Thr Val Ala Asn Arg Ser Leu Leu Leu Ile 
            580                 585                 590         


Gly Ser Pro Thr Trp Lys Asn Val Ser Arg Met Ala Arg Ser Ser His 
        595                 600                 605             


Lys Lys Asn Gln Glu Glu Lys Ser Leu Gly Lys Val Tyr Gly Tyr Phe 
    610                 615                 620                 


Leu Pro Asn Arg Gln Ser Thr Ile Thr Ile Ser Gly Asp Lys Ala Met 
625                 630                 635                 640 


Gly Lys Leu Gly Thr Ser Leu Ser Ser Gly Tyr Val Arg Val Asn Gly 
                645                 650                 655     


Thr Leu Thr Gln Val Leu Leu Val Gly Ala Pro Thr His Asp Asp Val 
            660                 665                 670         


Ser Lys Met Ala Phe Leu Thr Met Thr Leu His Gln Gly Gly Ala Thr 
        675                 680                 685             


Arg Met Tyr Glu Leu Ala Pro Glu Lys Thr Gln Pro Ala Leu Leu Ser 
    690                 695                 700                 


Thr Phe Ser Gly Asp Arg Arg Phe Ser Arg Phe Gly Ser Val Leu His 
705                 710                 715                 720 


Leu Thr Asp Leu Asp Asp Asp Gly Leu Asp Glu Ile Ile Met Ala Ala 
                725                 730                 735     


Pro Leu Arg Ile Thr Asp Val Thr Ser Gly Leu Leu Gly Gly Glu Asp 
            740                 745                 750         


Gly Arg Val Tyr Ile Tyr Asn Gly Met Tyr Thr Thr Leu Gly Asp Met 
        755                 760                 765             


Thr Gly Lys Cys Lys Ser Trp Met Thr Pro Cys Pro Glu Glu Lys Ala 
    770                 775                 780                 


Gln Tyr Val Leu Thr Ser Pro Glu Ala Ser Ser Arg Phe Gly Ser Ser 
785                 790                 795                 800 


Leu Val Ser Val Arg Ser Lys Gly Arg Asn Gln Val Val Val Ala Ala 
                805                 810                 815     


Gly Arg Ser Ser Trp Gly Ala Arg Leu Ser Gly Ala Leu His Val Tyr 
            820                 825                 830         


Ser Phe Ser Ser Asp 
        835         


<210> 3
<211> 12
<212> PRT
<213> Homo sapiens

<400> 3
Thr Val Tyr Leu His Leu Leu Asn Phe Leu Val Val 
1               5                   10          


<210> 4
<211> 12
<212> PRT
<213> Homo sapiens

<400> 4
Gly Asp Val Leu Ser Gln Phe Glu Phe Asn Phe Asn 
1               5                   10          


<210> 5
<211> 16
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      His tag


<220>
<221> MISC_FEATURE
<222> (1)..(16)
<223> This sequence may encompass 4-16 residues

<400> 5
His His His His His His His His His His His His His His His His 
1               5                   10                  15      


<210> 6
<211> 24
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 6
caccggagtg accatcaact ggca                                              24


<210> 7
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 7
tcaagaaagc ggggctgaat                                                   20


<210> 8
<211> 18
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 8
caccagtgtt gctgcact                                                     18


<210> 9
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 9
cactttcgat gacgtgcgtg                                                   20


<210> 10
<211> 24
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 10
ggaaagtcat caccaaagac gtcc                                              24


<210> 11
<211> 21
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 11
cagggcagct cacctacaat g                                                 21


<210> 12
<211> 31
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 12
gctgacgtga gctggaatag cctgggtatt g                                      31


<210> 13
<211> 31
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 13
caatacccag gctattccag ctcacgtcag c                                      31


<210> 14
<211> 34
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 14
tacaactctt actctgacgc taactcggct ggtg                                   34


<210> 15
<211> 34
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 15
caccagccga gttagcgtca gagtaagagt tgta                                   34


