                         SEQUENCE LISTING

<110>  Research Institute at Nationwide Children's Hospital
 
<120>  ADENO-ASSOCIATED VIRUS DELIVERY OF CLN6 POLYNUCLEOTIDE

<130>  33057/53894

<150>  US 62/800,915
<151>  2019-02-04

<150>  US 62/880,641
<151>  2019-07-30

<150>  US 62/881,151
<151>  2019-07-31

<150>  US 62/912,977
<151>  2019-10-09

<150>  US 62/923,125
<151>  2019-10-18

<160>  11    

<170>  PatentIn version 3.5

<210>  1
<211>  311
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<223>  CLN6 Polypeptide Sequence

<400>  1

Met Glu Ala Thr Arg Arg Arg Gln His Leu Gly Ala Thr Gly Gly Pro 
1               5                   10                  15      


Gly Ala Gln Leu Gly Ala Ser Phe Leu Gln Ala Arg His Gly Ser Val 
            20                  25                  30          


Ser Ala Asp Glu Ala Ala Arg Thr Ala Pro Phe His Leu Asp Leu Trp 
        35                  40                  45              


Phe Tyr Phe Thr Leu Gln Asn Trp Val Leu Asp Phe Gly Arg Pro Ile 
    50                  55                  60                  


Ala Met Leu Val Phe Pro Leu Glu Trp Phe Pro Leu Asn Lys Pro Ser 
65                  70                  75                  80  


Val Gly Asp Tyr Phe His Met Ala Tyr Asn Val Ile Thr Pro Phe Leu 
                85                  90                  95      


Leu Leu Lys Leu Ile Glu Arg Ser Pro Arg Thr Leu Pro Arg Ser Ile 
            100                 105                 110         


Thr Tyr Val Ser Ile Ile Ile Phe Ile Met Gly Ala Ser Ile His Leu 
        115                 120                 125             


Val Gly Asp Ser Val Asn His Arg Leu Leu Phe Ser Gly Tyr Gln His 
    130                 135                 140                 


His Leu Ser Val Arg Glu Asn Pro Ile Ile Lys Asn Leu Lys Pro Glu 
145                 150                 155                 160 


Thr Leu Ile Asp Ser Phe Glu Leu Leu Tyr Tyr Tyr Asp Glu Tyr Leu 
                165                 170                 175     


Gly His Cys Met Trp Tyr Ile Pro Phe Phe Leu Ile Leu Phe Met Tyr 
            180                 185                 190         


Phe Ser Gly Cys Phe Thr Ala Ser Lys Ala Glu Ser Leu Ile Pro Gly 
        195                 200                 205             


Pro Ala Leu Leu Leu Val Ala Pro Ser Gly Leu Tyr Tyr Trp Tyr Leu 
    210                 215                 220                 


Val Thr Glu Gly Gln Ile Phe Ile Leu Phe Ile Phe Thr Phe Phe Ala 
225                 230                 235                 240 


Met Leu Ala Leu Val Leu His Gln Lys Arg Lys Arg Leu Phe Leu Asp 
                245                 250                 255     


Ser Asn Gly Leu Phe Leu Phe Ser Ser Phe Ala Leu Thr Leu Leu Leu 
            260                 265                 270         


Val Ala Leu Trp Val Ala Trp Leu Trp Asn Asp Pro Val Leu Arg Lys 
        275                 280                 285             


Lys Tyr Pro Gly Val Ile Tyr Val Pro Glu Pro Trp Ala Phe Tyr Thr 
    290                 295                 300                 


Leu His Val Ser Ser Arg His 
305                 310     


<210>  2
<211>  936
<212>  DNA
<213>  Homo sapiens


<220>
<221>  misc_feature
<223>  CLN6  polynucleotide sequence

<400>  2
atggaggcga cgcggaggcg gcagcacctg ggagcgacgg gcggcccagg cgcgcagctg       60

ggcgcctcct tcctgcaggc caggcatggc tctgtgagcg ctgatgaggc tgcccgcacg      120

gctcccttcc acctcgacct ctggttctac ttcacactgc agaactgggt tctggacttt      180

gggcgtccca ttgccatgct ggtattccct ctcgagtggt ttccactcaa caagcccagt      240

gttggggact acttccacat ggcctacaac gtcatcacgc cctttctctt gctcaagctc      300

atcgagcggt ccccccgcac cctgccacgc tccatcacgt acgtgagcat catcatcttc      360

atcatgggtg ccagcatcca cctggtgggt gactctgtca accaccgcct gctcttcagt      420

ggctaccagc accacctgtc tgtccgtgag aaccccatca tcaagaatct caagccggag      480

acgctgatcg actcctttga gctgctctac tattatgatg agtacctggg tcactgcatg      540

tggtacatcc ccttcttcct catcctcttc atgtacttca gcggctgctt tactgcctct      600

aaagctgaga gcttgattcc agggcctgcc ctgctcctgg tggcacccag tggcctgtac      660

tactggtacc tggtcaccga gggccagatc ttcatcctct tcatcttcac cttcttcgcc      720

atgctggccc tcgtcctgca ccagaagcgc aagcgcctct tcctggacag caacggcctc      780

ttcctcttct cctccttcgc actgaccctc ttgcttgtgg cgctctgggt cgcctggctg      840

tggaatgacc ctgttctcag gaagaagtac ccgggtgtca tctacgtccc tgagccctgg      900

gctttctaca cccttcacgt cagcagtcgg cactga                                936


<210>  3
<211>  270
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleotide


<220>
<221>  misc_feature
<223>  hybrid chicken B -Actin promoter

<400>  3
ccacgttctg cttcactctc cccatctccc ccccctcccc acccccaatt ttgtatttat       60

ttatttttta attattttgt gcagcgatgg gggcgggggg gggggggggg cgcgcgccag      120

gcggggcggg gcggggcgag gggcggggcg gggcgaggcg gagaggtgcg gcggcagcca      180

atcagagcgg cgcgctccga aagtttcctt ttatggcgag gcggcggcgg cggcggccct      240

ataaaaagcg aagcgcgcgg cgggcgggag                                       270


<210>  4
<211>  2343
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleotide


<220>
<221>  misc_feature
<223>  Gene Cassette

<400>  4
ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt       60

ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggaatt cacgcgtgga      120

tctgaattca attcacgcgt ggtacctctg gtcgttacat aacttacggt aaatggcccg      180

cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata      240

gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc      300

cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac      360

ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg      420

cagtacatct actcgaggcc acgttctgct tcactctccc catctccccc ccctccccac      480

ccccaatttt gtatttattt attttttaat tattttgtgc agcgatgggg gcgggggggg      540

ggggggggcg cgcgccaggc ggggcggggc ggggcgaggg gcggggcggg gcgaggcgga      600

gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa gtttcctttt atggcgaggc      660

ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagcg ggatcagcca      720

ccgcggtggc ggcctagagt cgacgaggaa ctgaaaaacc agaaagttaa ctggtaagtt      780

tagtcttttt gtcttttatt tcaggtcccg gatccggtgg tggtgcaaat caaagaactg      840

ctcctcagtg gatgttgcct ttacttctag gcctgtacgg aagtgttact tctgctctaa      900

aagctgcgga attgtacccg cggccgatcc accggtctta agggccgagg cggccagatc      960

tttcgaagat atcggcgccg ctagcgcggc cgcatggagg cgacgcggag gcggcagcac     1020

ctgggagcga cgggcggccc aggcgcgcag ctgggcgcct ccttcctgca ggccaggcat     1080

ggctctgtga gcgctgatga ggctgcccgc acggctccct tccacctcga cctctggttc     1140

tacttcacac tgcagaactg ggttctggac tttgggcgtc ccattgccat gctggtattc     1200

cctctcgagt ggtttccact caacaagccc agtgttgggg actacttcca catggcctac     1260

aacgtcatca cgccctttct cttgctcaag ctcatcgagc ggtccccccg caccctgcca     1320

cgctccatca cgtacgtgag catcatcatc ttcatcatgg gtgccagcat ccacctggtg     1380

ggtgactctg tcaaccaccg cctgctcttc agtggctacc agcaccacct gtctgtccgt     1440

gagaacccca tcatcaagaa tctcaagccg gagacgctga tcgactcctt tgagctgctc     1500

tactattatg atgagtacct gggtcactgc atgtggtaca tccccttctt cctcatcctc     1560

ttcatgtact tcagcggctg ctttactgcc tctaaagctg agagcttgat tccagggcct     1620

gccctgctcc tggtggcacc cagtggcctg tactactggt acctggtcac cgagggccag     1680

atcttcatcc tcttcatctt caccttcttc gccatgctgg ccctcgtcct gcaccagaag     1740

cgcaagcgcc tcttcctgga cagcaacggc ctcttcctct tctcctcctt cgcactgacc     1800

ctcttgcttg tggcgctctg ggtcgcctgg ctgtggaatg accctgttct caggaagaag     1860

tacccgggtg tcatctacgt ccctgagccc tgggctttct acacccttca cgtcagcagt     1920

cggcactgag tctctagaaa gcttatcgat accgtcgact agagctcgct gatcagcctc     1980

gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac     2040

cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg     2100

tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga     2160

ttgggaagac aatagcaggc atgctgggga gagatcgatc tgaggaaccc ctagtgatgg     2220

agttggccac tccctctctg cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg     2280

cccgacgccc gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc agagagggag     2340

tgg                                                                   2343


<210>  5
<211>  6028
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleotide


<220>
<221>  misc_feature
<223>  AAV.CB.CLN6

<400>  5
ctgattctaa cgaggaaagc acgttatacg tgctcgtcaa agcaaccata gtacgcgccc       60

tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt      120

gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc      180

ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta      240

cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc      300

tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg      360

ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt      420

ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat      480

tttaacaaaa tattaacgct tacaatttaa atatttgctt atacaatctt cctgtttttg      540

gggcttttct gattatcaac cggggtacat atgattgaca tgctagtttt acgattaccg      600

ttcatcgccc tgcgcgctcg ctcgctcact gaggccgccc gggcaaagcc cgggcgtcgg      660

gcgacctttg gtcgcccggc ctcagtgagc gagcgagcgc gcagagaggg agtggaattc      720

acgcgtggat ctgaattcaa ttcacgcgtg gtacctctgg tcgttacata acttacggta      780

aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat      840

gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg      900

taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac      960

gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt     1020

cctacttggc agtacatcta ctcgaggcca cgttctgctt cactctcccc atctcccccc     1080

cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca gcgatggggg     1140

cggggggggg gggggggcgc gcgccaggcg gggcggggcg gggcgagggg cggggcgggg     1200

cgaggcggag aggtgcggcg gcagccaatc agagcggcgc gctccgaaag tttcctttta     1260

tggcgaggcg gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg gcgggagcgg     1320

gatcagccac cgcggtggcg gcctagagtc gacgaggaac tgaaaaacca gaaagttaac     1380

tggtaagttt agtctttttg tcttttattt caggtcccgg atccggtggt ggtgcaaatc     1440

aaagaactgc tcctcagtgg atgttgcctt tacttctagg cctgtacgga agtgttactt     1500

ctgctctaaa agctgcggaa ttgtacccgc ggccgatcca ccggtcttaa gggccgaggc     1560

ggccagatct ttcgaagata tcggcgccgc tagcgcggcc gcatggaggc gacgcggagg     1620

cggcagcacc tgggagcgac gggcggccca ggcgcgcagc tgggcgcctc cttcctgcag     1680

gccaggcatg gctctgtgag cgctgatgag gctgcccgca cggctccctt ccacctcgac     1740

ctctggttct acttcacact gcagaactgg gttctggact ttgggcgtcc cattgccatg     1800

ctggtattcc ctctcgagtg gtttccactc aacaagccca gtgttgggga ctacttccac     1860

atggcctaca acgtcatcac gccctttctc ttgctcaagc tcatcgagcg gtccccccgc     1920

accctgccac gctccatcac gtacgtgagc atcatcatct tcatcatggg tgccagcatc     1980

cacctggtgg gtgactctgt caaccaccgc ctgctcttca gtggctacca gcaccacctg     2040

tctgtccgtg agaaccccat catcaagaat ctcaagccgg agacgctgat cgactccttt     2100

gagctgctct actattatga tgagtacctg ggtcactgca tgtggtacat ccccttcttc     2160

ctcatcctct tcatgtactt cagcggctgc tttactgcct ctaaagctga gagcttgatt     2220

ccagggcctg ccctgctcct ggtggcaccc agtggcctgt actactggta cctggtcacc     2280

gagggccaga tcttcatcct cttcatcttc accttcttcg ccatgctggc cctcgtcctg     2340

caccagaagc gcaagcgcct cttcctggac agcaacggcc tcttcctctt ctcctccttc     2400

gcactgaccc tcttgcttgt ggcgctctgg gtcgcctggc tgtggaatga ccctgttctc     2460

aggaagaagt acccgggtgt catctacgtc cctgagccct gggctttcta cacccttcac     2520

gtcagcagtc ggcactgagt ctctagaaag cttatcgata ccgtcgacta gagctcgctg     2580

atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc     2640

ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc     2700

atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa     2760

gggggaggat tgggaagaca atagcaggca tgctggggag agatcgatct gaggaacccc     2820

tagtgatgga gttggccact ccctctctgc gcgctcgctc gctcactgag gccgggcgac     2880

caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc agtgagcgag cgagcgcgca     2940

gagagggagt ggcccccccc cccccccccc cggcgattct cttgtttgct ccagactctc     3000

aggcaatgac ctgatagcct ttgtagagac ctctcaaaaa tagctaccct ctccggcatg     3060

aatttatcag ctagaacggt tgaatatcat attgatggtg atttgactgt ctccggcctt     3120

tctcacccgt ttgaatcttt acctacacat tactcaggca ttgcatttaa aatatatgag     3180

ggttctaaaa atttttatcc ttgcgttgaa ataaaggctt ctcccgcaaa agtattacag     3240

ggtcataatg tttttggtac aaccgattta gctttatgct ctgaggcttt attgcttaat     3300

tttgctaatt ctttgccttg cctgtatgat ttattggatg ttggaatcgc ctgatgcggt     3360

attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa     3420

tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacacta tggtgcactc     3480

tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg     3540

ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg     3600

tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa     3660

agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga     3720

cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa     3780

tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt     3840

gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg     3900

cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag     3960

atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg     4020

agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg     4080

gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt     4140

ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga     4200

cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac     4260

ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc     4320

atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc     4380

gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac     4440

tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag     4500

gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg     4560

gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta     4620

tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg     4680

ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata     4740

tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt     4800

ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc     4860

ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct     4920

tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa     4980

ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag     5040

tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc     5100

tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg     5160

actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca     5220

cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat     5280

gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg     5340

tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc     5400

ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc     5460

ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc     5520

cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg     5580

cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga     5640

gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc     5700

attaatgcag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc     5760

gcagcctgaa tggcgaatgg cgattccgtt gcaatggctg gcggtaatat tgttctggat     5820

attaccagca aggccgatag tttgagttct tctactcagg caagtgatgt tattactaat     5880

caaagaagta ttgcgacaac ggttaatttg cgtgatggac agactctttt actcggtggc     5940

ctcactgatt ataaaaacac ttctcaggat tctggcgtac cgttcctgtc taaaatccct     6000

ttaatcggcc tcctgtttag ctcccgct                                        6028


<210>  6
<211>  280
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleotide


<220>
<221>  misc_feature
<223>  CMV Sequence

<400>  6
cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt       60

gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca      120

atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc      180

aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta      240

catgacctta tgggactttc ctacttggca gtacatctac                            280


<210>  7
<211>  736
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<223>  scAAV9 seuqence

<400>  7

Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser 
1               5                   10                  15      


Glu Gly Ile Arg Glu Trp Trp Ala Leu Lys Pro Gly Ala Pro Gln Pro 
            20                  25                  30          


Lys Ala Asn Gln Gln His Gln Asp Asn Ala Arg Gly Leu Val Leu Pro 
        35                  40                  45              


Gly Tyr Lys Tyr Leu Gly Pro Gly Asn Gly Leu Asp Lys Gly Glu Pro 
    50                  55                  60                  


Val Asn Ala Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp 
65                  70                  75                  80  


Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala 
                85                  90                  95      


Asp Ala Glu Phe Gln Glu Arg Leu Lys Glu Asp Thr Ser Phe Gly Gly 
            100                 105                 110         


Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Leu Leu Glu Pro 
        115                 120                 125             


Leu Gly Leu Val Glu Glu Ala Ala Lys Thr Ala Pro Gly Lys Lys Arg 
    130                 135                 140                 


Pro Val Glu Gln Ser Pro Gln Glu Pro Asp Ser Ser Ala Gly Ile Gly 
145                 150                 155                 160 


Lys Ser Gly Ala Gln Pro Ala Lys Lys Arg Leu Asn Phe Gly Gln Thr 
                165                 170                 175     


Gly Asp Thr Glu Ser Val Pro Asp Pro Gln Pro Ile Gly Glu Pro Pro 
            180                 185                 190         


Ala Ala Pro Ser Gly Val Gly Ser Leu Thr Met Ala Ser Gly Gly Gly 
        195                 200                 205             


Ala Pro Val Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Ser Ser 
    210                 215                 220                 


Ser Gly Asn Trp His Cys Asp Ser Gln Trp Leu Gly Asp Arg Val Ile 
225                 230                 235                 240 


Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu 
                245                 250                 255     


Tyr Lys Gln Ile Ser Asn Ser Thr Ser Gly Gly Ser Ser Asn Asp Asn 
            260                 265                 270         


Ala Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg 
        275                 280                 285             


Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn 
    290                 295                 300                 


Asn Trp Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn Ile 
305                 310                 315                 320 


Gln Val Lys Glu Val Thr Asp Asn Asn Gly Val Lys Thr Ile Ala Asn 
                325                 330                 335     


Asn Leu Thr Ser Thr Val Gln Val Phe Thr Asp Ser Asp Tyr Gln Leu 
            340                 345                 350         


Pro Tyr Val Leu Gly Ser Ala His Glu Gly Cys Leu Pro Pro Phe Pro 
        355                 360                 365             


Ala Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asp 
    370                 375                 380                 


Gly Ser Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe 
385                 390                 395                 400 


Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Gln Phe Ser Tyr Glu 
                405                 410                 415     


Phe Glu Asn Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu 
            420                 425                 430         


Asp Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Ser 
        435                 440                 445             


Lys Thr Ile Asn Gly Ser Gly Gln Asn Gln Gln Thr Leu Lys Phe Ser 
    450                 455                 460                 


Val Ala Gly Pro Ser Asn Met Ala Val Gln Gly Arg Asn Tyr Ile Pro 
465                 470                 475                 480 


Gly Pro Ser Tyr Arg Gln Gln Arg Val Ser Thr Thr Val Thr Gln Asn 
                485                 490                 495     


Asn Asn Ser Glu Phe Ala Trp Pro Gly Ala Ser Ser Trp Ala Leu Asn 
            500                 505                 510         


Gly Arg Asn Ser Leu Met Asn Pro Gly Pro Ala Met Ala Ser His Lys 
        515                 520                 525             


Glu Gly Glu Asp Arg Phe Phe Pro Leu Ser Gly Ser Leu Ile Phe Gly 
    530                 535                 540                 


Lys Gln Gly Thr Gly Arg Asp Asn Val Asp Ala Asp Lys Val Met Ile 
545                 550                 555                 560 


Thr Asn Glu Glu Glu Ile Lys Thr Thr Asn Pro Val Ala Thr Glu Ser 
                565                 570                 575     


Tyr Gly Gln Val Ala Thr Asn His Gln Ser Ala Gln Ala Gln Ala Gln 
            580                 585                 590         


Thr Gly Trp Val Gln Asn Gln Gly Ile Leu Pro Gly Met Val Trp Gln 
        595                 600                 605             


Asp Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro His 
    610                 615                 620                 


Thr Asp Gly Asn Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly Met 
625                 630                 635                 640 


Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro Ala 
                645                 650                 655     


Asp Pro Pro Thr Ala Phe Asn Lys Asp Lys Leu Asn Ser Phe Ile Thr 
            660                 665                 670         


Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu Gln 
        675                 680                 685             


Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn 
    690                 695                 700                 


Tyr Tyr Lys Ser Asn Asn Val Glu Phe Ala Val Asn Thr Glu Gly Val 
705                 710                 715                 720 


Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn Leu 
                725                 730                 735     


<210>  8
<211>  141
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleotide


<220>
<221>  misc_feature
<223>  AAV2 3' ITR

<400>  8
aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg ctcactgagg       60

ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc      120

gagcgcgcag agagggagtg g                                                141


<210>  9
<211>  106
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleotide


<220>
<221>  misc_feature
<223>  AAV2 5' ITR

<400>  9
ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt       60

ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtgg                     106


<210>  10
<211>  147
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polyucleotide


<220>
<221>  misc_feature
<223>  BGH Poly A

<400>  10
cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct       60

tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc      120

attgtctgag taggtgtcat tctattc                                          147


<210>  11
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleotide


<220>
<221>  misc_feature
<223>  SV40 intron

<400>  11
gtaagtttag tctttttgtc ttttatttca ggtcccggat ccggtggtgg tgcaaatcaa       60


