                         SEQUENCE LISTING

<110>  THE UNITED STATES OF AMERICA, AS REPRESENTED BY THE 
       SECRETARY, DEPARTMENT OF HEALTH AND HUMAN SERVICES
 
<120>  DETECTION OF BCL-2 FAMILY HETERODIMER COMPLEXES AND USE THEREOF

<130>  4239-101599-02

<150>  US 62/798,615
<151>  2019-01-30

<160>  5     

<170>  PatentIn version 3.5

<210>  1
<211>  442
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Fusion protein (BIM-Bcl-2)

<400>  1

Gly Ala Lys Gln Pro Ser Asp Val Ser Ser Glu Cys Asp Arg Glu Gly 
1               5                   10                  15      


Arg Gln Leu Gln Pro Ala Glu Arg Pro Pro Gln Leu Arg Pro Gly Ala 
            20                  25                  30          


Pro Thr Ser Leu Gln Thr Glu Pro Gln Gly Asn Pro Glu Gly Asn His 
        35                  40                  45              


Gly Gly Glu Gly Asp Ser Cys Pro His Gly Ser Pro Gln Gly Pro Leu 
    50                  55                  60                  


Ala Pro Pro Ala Ser Pro Gly Pro Phe Ala Thr Arg Ser Pro Leu Phe 
65                  70                  75                  80  


Ile Phe Met Arg Arg Ser Ser Leu Leu Ser Arg Ser Ser Ser Gly Tyr 
                85                  90                  95      


Phe Ser Phe Asp Thr Asp Arg Ser Pro Ala Pro Met Ser Cys Asp Lys 
            100                 105                 110         


Ser Thr Gln Thr Pro Ser Pro Pro Cys Gln Ala Phe Asn His Tyr Leu 
        115                 120                 125             


Ser Ala Met Ala Ser Met Arg Gln Ala Glu Pro Ala Asp Met Arg Pro 
    130                 135                 140                 


Glu Ile Trp Ile Ala Gln Glu Leu Arg Arg Ile Gly Asp Glu Phe Asn 
145                 150                 155                 160 


Ala Tyr Tyr Ala Arg Arg Val Phe Leu Asn Asn Tyr Gln Ala Ala Glu 
                165                 170                 175     


Asp His Pro Arg Met Val Ile Leu Arg Leu Leu Arg Tyr Ile Val Arg 
            180                 185                 190         


Leu Val Trp Arg Met His Gly Ser Gly Ala Gly Gly Ser Ala Gly Gly 
        195                 200                 205             


Ser Gly Ala Gly Ser Gly Ala Gly Ser Gly Ala His Ala Gly Arg Thr 
    210                 215                 220                 


Gly Tyr Asp Asn Arg Glu Ile Val Met Lys Tyr Ile His Tyr Lys Leu 
225                 230                 235                 240 


Ser Gln Arg Gly Tyr Glu Trp Asp Ala Gly Asp Val Gly Ala Ala Pro 
                245                 250                 255     


Pro Gly Ala Ala Pro Ala Pro Gly Ile Phe Ser Ser Gln Pro Gly His 
            260                 265                 270         


Thr Pro His Pro Ala Ala Ser Arg Asp Pro Val Ala Arg Thr Ser Pro 
        275                 280                 285             


Leu Gln Thr Pro Ala Ala Pro Gly Ala Ala Ala Gly Pro Ala Leu Ser 
    290                 295                 300                 


Pro Val Pro Pro Val Val His Leu Thr Leu Arg Gln Ala Gly Asp Asp 
305                 310                 315                 320 


Phe Ser Arg Arg Tyr Arg Arg Asp Phe Ala Glu Met Ser Ser Gln Leu 
                325                 330                 335     


His Leu Thr Pro Phe Thr Ala Arg Gly Arg Phe Ala Thr Val Val Glu 
            340                 345                 350         


Glu Leu Phe Arg Asp Gly Val Asn Trp Gly Arg Ile Val Ala Phe Phe 
        355                 360                 365             


Glu Phe Gly Gly Val Met Cys Val Glu Ser Val Asn Arg Glu Met Ser 
    370                 375                 380                 


Pro Leu Val Asp Asn Ile Ala Leu Trp Met Thr Glu Tyr Leu Asn Arg 
385                 390                 395                 400 


His Leu His Thr Trp Ile Gln Asp Asn Gly Gly Trp Asp Ala Phe Val 
                405                 410                 415     


Glu Leu Tyr Gly Pro Ser Met Arg Pro Leu Phe Asp Phe Ser Gly Gly 
            420                 425                 430         


Ser Trp Ser Gly Pro Gln Phe Glu Lys Gly 
        435                 440         


<210>  2
<211>  441
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Fusion protein (BIM-Bcl-xL)

<400>  2

Gly Ala Lys Gln Pro Ser Asp Val Ser Ser Glu Cys Asp Arg Glu Gly 
1               5                   10                  15      


Arg Gln Leu Gln Pro Ala Glu Arg Pro Pro Gln Leu Arg Pro Gly Ala 
            20                  25                  30          


Pro Thr Ser Leu Gln Thr Glu Pro Gln Gly Asn Pro Glu Gly Asn His 
        35                  40                  45              


Gly Gly Glu Gly Asp Ser Cys Pro His Gly Ser Pro Gln Gly Pro Leu 
    50                  55                  60                  


Ala Pro Pro Ala Ser Pro Gly Pro Phe Ala Thr Arg Ser Pro Leu Phe 
65                  70                  75                  80  


Ile Phe Met Arg Arg Ser Ser Leu Leu Ser Arg Ser Ser Ser Gly Tyr 
                85                  90                  95      


Phe Ser Phe Asp Thr Asp Arg Ser Pro Ala Pro Met Ser Cys Asp Lys 
            100                 105                 110         


Ser Thr Gln Thr Pro Ser Pro Pro Cys Gln Ala Phe Asn His Tyr Leu 
        115                 120                 125             


Ser Ala Met Ala Ser Met Arg Gln Ala Glu Pro Ala Asp Met Arg Pro 
    130                 135                 140                 


Glu Ile Trp Ile Ala Gln Glu Leu Arg Arg Ile Gly Asp Glu Phe Asn 
145                 150                 155                 160 


Ala Tyr Tyr Ala Arg Arg Val Phe Leu Asn Asn Tyr Gln Ala Ala Glu 
                165                 170                 175     


Asp His Pro Arg Met Val Ile Leu Arg Leu Leu Arg Tyr Ile Val Arg 
            180                 185                 190         


Leu Val Trp Arg Met His Gly Ser Gly Ala Gly Gly Ser Ala Gly Gly 
        195                 200                 205             


Ser Gly Ala Gly Ser Gly Ala Gly Ser Gly Ser Gln Ser Asn Arg Glu 
    210                 215                 220                 


Leu Val Val Asp Phe Leu Ser Tyr Lys Leu Ser Gln Lys Gly Tyr Ser 
225                 230                 235                 240 


Trp Ser Gln Phe Ser Asp Val Glu Glu Asn Arg Thr Glu Ala Pro Glu 
                245                 250                 255     


Gly Thr Glu Ser Glu Met Glu Thr Pro Ser Ala Ile Asn Gly Asn Pro 
            260                 265                 270         


Ser Trp His Leu Ala Asp Ser Pro Ala Val Asn Gly Ala Thr Gly His 
        275                 280                 285             


Ser Ser Ser Leu Asp Ala Arg Glu Val Ile Pro Met Ala Ala Val Lys 
    290                 295                 300                 


Gln Ala Leu Arg Glu Ala Gly Asp Glu Phe Glu Leu Arg Tyr Arg Arg 
305                 310                 315                 320 


Ala Phe Ser Asp Leu Thr Ser Gln Leu His Ile Thr Pro Gly Thr Ala 
                325                 330                 335     


Tyr Gln Ser Phe Glu Gln Val Val Asn Glu Leu Phe Arg Asp Gly Val 
            340                 345                 350         


Asn Trp Gly Arg Ile Val Ala Phe Phe Ser Phe Gly Gly Ala Leu Cys 
        355                 360                 365             


Val Glu Ser Val Asp Lys Glu Met Gln Val Leu Val Ser Arg Ile Ala 
    370                 375                 380                 


Ala Trp Met Ala Thr Tyr Leu Asn Asp His Leu Glu Pro Trp Ile Gln 
385                 390                 395                 400 


Glu Asn Gly Gly Trp Asp Thr Phe Val Glu Leu Tyr Gly Asn Asn Ala 
                405                 410                 415     


Ala Ala Glu Ser Arg Lys Gly Gln Glu Arg Phe Asn Arg Gly Gly Ser 
            420                 425                 430         


Trp Ser Gly Pro Gln Phe Glu Lys Gly 
        435                 440     


<210>  3
<211>  579
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Fusion protein (BIM-Mcl-1)

<400>  3

Gly Ala Lys Gln Pro Ser Asp Val Ser Ser Glu Cys Asp Arg Glu Gly 
1               5                   10                  15      


Arg Gln Leu Gln Pro Ala Glu Arg Pro Pro Gln Leu Arg Pro Gly Ala 
            20                  25                  30          


Pro Thr Ser Leu Gln Thr Glu Pro Gln Gly Asn Pro Glu Gly Asn His 
        35                  40                  45              


Gly Gly Glu Gly Asp Ser Cys Pro His Gly Ser Pro Gln Gly Pro Leu 
    50                  55                  60                  


Ala Pro Pro Ala Ser Pro Gly Pro Phe Ala Thr Arg Ser Pro Leu Phe 
65                  70                  75                  80  


Ile Phe Met Arg Arg Ser Ser Leu Leu Ser Arg Ser Ser Ser Gly Tyr 
                85                  90                  95      


Phe Ser Phe Asp Thr Asp Arg Ser Pro Ala Pro Met Ser Cys Asp Lys 
            100                 105                 110         


Ser Thr Gln Thr Pro Ser Pro Pro Cys Gln Ala Phe Asn His Tyr Leu 
        115                 120                 125             


Ser Ala Met Ala Ser Met Arg Gln Ala Glu Pro Ala Asp Met Arg Pro 
    130                 135                 140                 


Glu Ile Trp Ile Ala Gln Glu Leu Arg Arg Ile Gly Asp Glu Phe Asn 
145                 150                 155                 160 


Ala Tyr Tyr Ala Arg Arg Val Phe Leu Asn Asn Tyr Gln Ala Ala Glu 
                165                 170                 175     


Asp His Pro Arg Met Val Ile Leu Arg Leu Leu Arg Tyr Ile Val Arg 
            180                 185                 190         


Leu Val Trp Arg Met His Gly Ser Gly Ala Gly Gly Ser Ala Gly Gly 
        195                 200                 205             


Ser Gly Ala Gly Ser Gly Ala Gly Ser Gly Phe Gly Leu Lys Arg Asn 
    210                 215                 220                 


Ala Val Ile Gly Leu Asn Leu Tyr Cys Gly Gly Ala Gly Leu Gly Ala 
225                 230                 235                 240 


Gly Ser Gly Gly Ala Thr Arg Pro Gly Gly Arg Leu Leu Ala Thr Glu 
                245                 250                 255     


Lys Glu Ala Ser Ala Arg Arg Glu Ile Gly Gly Gly Glu Ala Gly Ala 
            260                 265                 270         


Val Ile Gly Gly Ser Ala Gly Ala Ser Pro Pro Ser Thr Leu Thr Pro 
        275                 280                 285             


Asp Ser Arg Arg Val Ala Arg Pro Pro Pro Ile Gly Ala Glu Val Pro 
    290                 295                 300                 


Asp Val Thr Ala Thr Pro Ala Arg Leu Leu Phe Phe Ala Pro Thr Arg 
305                 310                 315                 320 


Arg Ala Ala Pro Leu Glu Glu Met Glu Ala Pro Ala Ala Asp Ala Ile 
                325                 330                 335     


Met Ser Pro Glu Glu Glu Leu Asp Gly Tyr Glu Pro Glu Pro Leu Gly 
            340                 345                 350         


Lys Arg Pro Ala Val Leu Pro Leu Leu Glu Leu Val Gly Glu Ser Gly 
        355                 360                 365             


Asn Asn Thr Ser Thr Asp Gly Ser Leu Pro Ser Thr Pro Pro Pro Ala 
    370                 375                 380                 


Glu Glu Glu Glu Asp Glu Leu Tyr Arg Gln Ser Leu Glu Ile Ile Ser 
385                 390                 395                 400 


Arg Tyr Leu Arg Glu Gln Ala Thr Gly Ala Lys Asp Thr Lys Pro Met 
                405                 410                 415     


Gly Arg Ser Gly Ala Thr Ser Arg Lys Ala Leu Glu Thr Leu Arg Arg 
            420                 425                 430         


Val Gly Asp Gly Val Gln Arg Asn His Glu Thr Ala Phe Gln Gly Met 
        435                 440                 445             


Leu Arg Lys Leu Asp Ile Lys Asn Glu Asp Asp Val Lys Ser Leu Ser 
    450                 455                 460                 


Arg Val Met Ile His Val Phe Ser Asp Gly Val Thr Asn Trp Gly Arg 
465                 470                 475                 480 


Ile Val Thr Leu Ile Ser Phe Gly Ala Phe Val Ala Lys His Leu Lys 
                485                 490                 495     


Thr Ile Asn Gln Glu Ser Cys Ile Glu Pro Leu Ala Glu Ser Ile Thr 
            500                 505                 510         


Asp Val Leu Val Arg Thr Lys Arg Asp Trp Leu Val Lys Gln Arg Gly 
        515                 520                 525             


Trp Asp Gly Phe Val Glu Phe Phe His Val Glu Asp Leu Glu Gly Gly 
    530                 535                 540                 


Ile Arg Asn Val Leu Leu Ala Phe Ala Gly Val Ala Gly Val Gly Ala 
545                 550                 555                 560 


Gly Leu Ala Tyr Leu Ile Arg Gly Gly Ser Trp Ser Gly Pro Gln Phe 
                565                 570                 575     


Glu Lys Gly 
            


<210>  4
<211>  383
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Fusion protein (BAX-BAK)

<400>  4

Gly Ala Ser Gly Gln Gly Pro Gly Pro Pro Arg Gln Glu Cys Gly Glu 
1               5                   10                  15      


Pro Ala Leu Pro Ser Ala Ser Glu Glu Gln Val Ala Gln Asp Thr Glu 
            20                  25                  30          


Glu Val Phe Arg Ser Tyr Val Phe Tyr Arg His Gln Gln Glu Gln Glu 
        35                  40                  45              


Ala Glu Gly Val Ala Ala Pro Ala Asp Pro Glu Met Val Thr Leu Pro 
    50                  55                  60                  


Leu Gln Pro Ser Ser Thr Met Gly Gln Val Gly Arg Gln Leu Ala Ile 
65                  70                  75                  80  


Ile Gly Asp Asp Ile Asn Arg Arg Tyr Asp Ser Glu Phe Gln Thr Met 
                85                  90                  95      


Leu Gln His Leu Gln Pro Thr Ala Glu Asn Ala Tyr Glu Tyr Phe Thr 
            100                 105                 110         


Lys Ile Ala Thr Ser Leu Phe Glu Ser Gly Ile Asn Trp Gly Arg Val 
        115                 120                 125             


Val Ala Leu Leu Gly Phe Gly Tyr Arg Leu Ala Leu His Val Tyr Gln 
    130                 135                 140                 


His Gly Leu Thr Gly Phe Leu Gly Gln Val Thr Arg Phe Val Val Asp 
145                 150                 155                 160 


Phe Met Leu His His Cys Ile Ala Arg Trp Ile Ala Gln Arg Gly Gly 
                165                 170                 175     


Trp Val Ala Ala Leu Asn Leu Gly Asn Gly Ser Gly Ala Gly Gly Ser 
            180                 185                 190         


Ala Gly Gly Ser Gly Ala Gly Ser Gly Ala Gly Ser Gly Asp Gly Ser 
        195                 200                 205             


Gly Glu Gln Pro Arg Gly Gly Gly Pro Thr Ser Ser Glu Gln Ile Met 
    210                 215                 220                 


Lys Thr Gly Ala Leu Leu Leu Gln Gly Phe Ile Gln Asp Arg Ala Gly 
225                 230                 235                 240 


Arg Met Gly Gly Glu Ala Pro Glu Leu Ala Leu Asp Pro Val Pro Gln 
                245                 250                 255     


Asp Ala Ser Thr Lys Lys Leu Ser Glu Cys Leu Lys Arg Ile Gly Asp 
            260                 265                 270         


Glu Leu Asp Ser Asn Met Glu Leu Gln Arg Met Ile Ala Ala Val Asp 
        275                 280                 285             


Thr Asp Ser Pro Arg Glu Val Phe Phe Arg Val Ala Ala Asp Met Phe 
    290                 295                 300                 


Ser Asp Gly Asn Phe Asn Trp Gly Arg Val Val Ala Leu Phe Tyr Phe 
305                 310                 315                 320 


Ala Ser Lys Leu Val Leu Lys Ala Leu Cys Thr Lys Val Pro Glu Leu 
                325                 330                 335     


Ile Arg Thr Ile Met Gly Trp Thr Leu Asp Phe Leu Arg Glu Arg Leu 
            340                 345                 350         


Leu Gly Trp Ile Gln Asp Gln Gly Gly Trp Asp Gly Leu Leu Ser Tyr 
        355                 360                 365             


Phe Gly Thr Gly Gly Ser Trp Ser Gly Pro Gln Phe Glu Lys Gly 
    370                 375                 380             


<210>  5
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic polypeptide

<400>  5

Gly Ser Gly Ala Gly Gly Ser Ala Gly Gly Ser Gly Ala Gly Ser Gly 
1               5                   10                  15      


Ala Gly Ser Gly 
            20  


