                         SEQUENCE LISTING

<110>  Bar Ilan University
       Yissum Research Development Company of the Hebrew 
       University of Jerusalem Ltd.
 
<120>  NEOANTIGENS CREATED BY ABERRANT-INDUCED SPLICING AND USES THEREOF
        IN ENHANCING IMMUNOTHERAPY

<130>  2690533

<150>  US 62/799,377
<151>  2019-01-31

<160>  89    

<170>  PatentIn version 3.5

<210>  1
<211>  182
<212>  DNA
<213>  Homo sapiens


<220>
<221>  misc_feature
<223>  TYR exon 4, of length 182bp from transcript NM_000372.4_3 (HG38)

<400>  1
tatttttgag cagtggctcc gaaggcaccg tcctcttcaa gaagtttatc cagaagccaa       60

tgcacccatt ggacataacc gggaatccta catggttcct tttataccac tgtacagaaa      120

tggtgatttc tttatttcat ccaaagatct gggctatgac tatagctatc tacaagattc      180

ag                                                                     182


<210>  2
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  New amino acids at the C-terminus of aberrant TYR isoform 
       (derived from human)

<400>  2

Arg Pro Arg Leu Phe Ser Arg Leu His 
1               5                   


<210>  3
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Last 17 residues of the new aberrant TYR isoform (derived from 
       human)

<400>  3

Leu Leu His His Ala Phe Val Asp Arg Pro Arg Leu Phe Ser Arg Leu 
1               5                   10                  15      


His 
    


<210>  4
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  TYR oligo 9 (designed for mouse)

<400>  4
gagggcggug ccuucgcagc                                                   20


<210>  5
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  TYR oligo 13, (designed for mouse)

<400>  5
ucuuggaggu agcuguaguc                                                   20


<210>  6
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligo 9 target sequence in mouse TYR

<400>  6
gctgcgaagg caccgccctc                                                   20


<210>  7
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligos 13 target sequence in mouse TYR

<400>  7
gactacagct acctccaaga                                                   20


<210>  8
<211>  182
<212>  DNA
<213>  Mus musculus


<220>
<221>  misc_feature
<223>  TYR exon 4, of length 182bp from transcript NM_001317397.1_2

<400>  8
tatttttgaa caatggctgc gaaggcaccg ccctcttttg gaagtttacc cagaagccaa       60

tgcacctatc ggccataaca gagactctta catggttcct ttcataccgc tctatagaaa      120

tggtgatttc ttcataacat ccaaggatct gggatatgac tacagctacc tccaagagtc      180

ag                                                                     182


<210>  9
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  New amino acids at the C-terminus of the aberrant TYR isoform 
       (derived from mouse)

<400>  9

Arg Ser Arg Leu Leu Gln Lys Leu Tyr 
1               5                   


<210>  10
<211>  2065
<212>  DNA
<213>  Homo sapiens


<220>
<221>  misc_feature
<223>  TYR transcript NM_000372.4 (mRNA including UTRs) (HG38)

<400>  10
atcactgtag tagtagctgg aaagagaaat ctgtgactcc aattagccag ttcctgcaga       60

ccttgtgagg actagaggaa gaatgctcct ggctgttttg tactgcctgc tgtggagttt      120

ccagacctcc gctggccatt tccctagagc ctgtgtctcc tctaagaacc tgatggagaa      180

ggaatgctgt ccaccgtgga gcggggacag gagtccctgt ggccagcttt caggcagagg      240

ttcctgtcag aatatccttc tgtccaatgc accacttggg cctcaatttc ccttcacagg      300

ggtggatgac cgggagtcgt ggccttccgt cttttataat aggacctgcc agtgctctgg      360

caacttcatg ggattcaact gtggaaactg caagtttggc ttttggggac caaactgcac      420

agagagacga ctcttggtga gaagaaacat cttcgatttg agtgccccag agaaggacaa      480

attttttgcc tacctcactt tagcaaagca taccatcagc tcagactatg tcatccccat      540

agggacctat ggccaaatga aaaatggatc aacacccatg tttaacgaca tcaatattta      600

tgacctcttt gtctggatgc attattatgt gtcaatggat gcactgcttg ggggatctga      660

aatctggaga gacattgatt ttgcccatga agcaccagct tttctgcctt ggcatagact      720

cttcttgttg cggtgggaac aagaaatcca gaagctgaca ggagatgaaa acttcactat      780

tccatattgg gactggcggg atgcagaaaa gtgtgacatt tgcacagatg agtacatggg      840

aggtcagcac cccacaaatc ctaacttact cagcccagca tcattcttct cctcttggca      900

gattgtctgt agccgattgg aggagtacaa cagccatcag tctttatgca atggaacgcc      960

cgagggacct ttacggcgta atcctggaaa ccatgacaaa tccagaaccc caaggctccc     1020

ctcttcagct gatgtagaat tttgcctgag tttgacccaa tatgaatctg gttccatgga     1080

taaagctgcc aatttcagct ttagaaatac actggaagga tttgctagtc cacttactgg     1140

gatagcggat gcctctcaaa gcagcatgca caatgccttg cacatctata tgaatggaac     1200

aatgtcccag gtacagggat ctgccaacga tcctatcttc cttcttcacc atgcatttgt     1260

tgacagtatt tttgagcagt ggctccgaag gcaccgtcct cttcaagaag tttatccaga     1320

agccaatgca cccattggac ataaccggga atcctacatg gttcctttta taccactgta     1380

cagaaatggt gatttcttta tttcatccaa agatctgggc tatgactata gctatctaca     1440

agattcagac ccagactctt ttcaagacta cattaagtcc tatttggaac aagcgagtcg     1500

gatctggtca tggctccttg gggcggcgat ggtaggggcc gtcctcactg ccctgctggc     1560

agggcttgtg agcttgctgt gtcgtcacaa gagaaagcag cttcctgaag aaaagcagcc     1620

actcctcatg gagaaagagg attaccacag cttgtatcag agccatttat aaaaggctta     1680

ggcaatagag tagggccaaa aagcctgacc tcactctaac tcaaagtaat gtccaggttc     1740

ccagagaata tctgctggta tttttctgta aagaccattt gcaaaattgt aacctaatac     1800

aaagtgtagc cttcttccaa ctcaggtaga acacacctgt ctttgtcttg ctgttttcac     1860

tcagcccttt taacattttc ccctaagccc atatgtctaa ggaaaggatg ctatttggta     1920

atgaggaact gttatttgta tgtgaattaa agtgctctta ttttaaaaaa ttgaaataat     1980

tttgattttt gccttctgat tatttaaaga tctatatatg ttttattggc cccttcttta     2040

ttttaataaa acagtgagaa atcta                                           2065


<210>  11
<211>  6042
<212>  DNA
<213>  Mus musculus


<220>
<221>  misc_feature
<223>  TYR transcript NM_011661.5 (mRNA including UTRs)

<400>  11
aagcttagtg taaaacaggc tgagagtatt tgatgtaaga aggggagtgg ttatataggt       60

cttagccaaa acatgtgata gtcactccag gggttgctgg aaaagaagtc tgtgacactc      120

attaacctat tggtgcagat tttgtatgat ctaaaggaga aaatgttctt ggctgttttg      180

tattgccttc tgtggagttt ccagatctct gatggccatt ttcctcgagc ctgtgcctcc      240

tctaagaact tgttggcaaa agaatgctgc ccaccatgga tgggtgatgg gagtccctgc      300

ggccagcttt caggcagagg ttcctgccag gatatccttc tgtccagtgc accatctgga      360

cctcagttcc ccttcaaagg ggtggatgac cgtgagtcct ggccctctgt gttttataat      420

aggacctgcc agtgctcagg caacttcatg ggtttcaact gcggaaactg taagtttgga      480

tttgggggcc caaattgtac agagaagcga gtcttgatta gaagaaacat ttttgatttg      540

agtgtctccg aaaagaataa gttcttttct tacctcactt tagcaaaaca tactatcagc      600

tcagtctatg tcatccccac aggcacctat ggccaaatga acaatgggtc aacacccatg      660

tttaatgata tcaacatcta cgacctcttt gtatggatgc attactatgt gtcaagggac      720

acactgcttg ggggctctga aatatggagg gacattgatt ttgcccatga agcaccaggg      780

tttctgcctt ggcacagact tttcttgtta ttgtgggaac aagaaattcg agaactaact      840

ggggatgaga acttcactgt tccatactgg gattggagag atgcagaaaa ctgtgacatt      900

tgcacagatg agtacttggg aggtcgtcac cctgaaaatc ctaacttact cagcccagca      960

tccttcttct cctcctggca gatcatttgt agcagatcag aagagtataa tagccatcag     1020

gttttatgcg atggaacacc tgagggacca ctattacgta atcctggaaa ccatgacaaa     1080

gccaaaaccc ccaggctccc atcttcagca gatgtggaat tttgtctgag tttgacccag     1140

tatgaatctg gatcaatgga tagaactgcc aatttcagct ttagaaacac actggaagga     1200

tttgccagtc cactcacagg gatagcagat ccttctcaaa gtagcatgca caatgcctta     1260

catatcttta tgaatggaac aatgtcccaa gtacagggat cggccaacga tcccattttt     1320

cttcttcacc atgcttttgt ggacagtatt tttgaacaat ggctgcgaag gcaccgccct     1380

cttttggaag tttacccaga agccaatgca cctatcggcc ataacagaga ctcttacatg     1440

gttcctttca taccgctcta tagaaatggt gatttcttca taacatccaa ggatctggga     1500

tatgactaca gctacctcca agagtcagat ccaggctttt acagaaatta tattgagcct     1560

tacttggaac aagccagtcg tatctggcca tggcttcttg gggcagcact ggtgggagct     1620

gttattgctg cagctctctc tgggcttagc agtaggctat gccttcagaa gaagaagaag     1680

aagaagcaac cccaggagga aaggcagcca ctcctcatgg acaaagacga ctaccacagc     1740

ttgctgtatc agagccatct gtgaacatcc taggaaacag agtgggactg aaaggtttta     1800

cctcactcga cctatttgtt ggtgtttcta caaatttaaa ctagtataaa acatagacca     1860

tagctgtttg gctttttttc agacccatgt tttttcctaa gtcctagttt ctaagaaatg     1920

actgggattt gctaaaatat atatatatat ataaataata acttactaat agctaaataa     1980

aatttcctct tacaactaat tgagctggtt tttatgaatg tgtcttaatt atttaaactt     2040

gaggcacatt tttgttttcc ttacttcatt gtgaatttcc aagaaaaata ttctctctct     2100

ctctctctct cgtgtgtttg tgtgtatgtg tgtgttaact gattcaaaca attttgaaaa     2160

tcttggattg atagaaatga ttcattaatt tatgaaatta tttcattaat gattaggaaa     2220

gacgaataat tactaaatta gtaacagagg agaacatctg ccagctttta ttaattgtca     2280

tttaagttac cttatctacc ttctgtgact ggtggaaaaa tatcaggcaa gagatgggaa     2340

tgctctgcct aataggatag tggctcctgg aaggagtggg ttattactag agattattac     2400

ctgaagttta ccatagccag aaaattaatc aaaacagatg actcagtaac atctgaagct     2460

tcaagtcggc ttgactgcaa tctgaaatca tcaagcccaa gagccaaagg aatgggaaca     2520

gcgatgggaa actatctgaa tcagattcta gtgtgatagt gtcaggggca catgggtcat     2580

ctttgagacc ttcacacctg ttgagtcacc aaaatttgct gtgaatgtaa atttttactg     2640

taaattaatt ttttcttttc tttttaaaaa gatttattta ttattataca taagtacact     2700

gtagctgtct tcagacacac cagaagaggg tgtcagatct cattacagat ggttgtgagc     2760

caccatgtgg ttgctgggat ttgaactcag gacctctgga agaacagtca gtgctcttac     2820

ccgctgagcc atctcgccag cccagtaaat ttttacttta gtgaaagtaa aatttaagtt     2880

ttagttttta gtttagtaaa attttaggaa gcaaattttt agttttctaa actaattttt     2940

ttttctagta ctggacatca acccagtgcc ttgtatatgc aatgcaagca ttttcttgta     3000

ctctgctacc tagcatgtat atataaatct acccaacaaa tgttcattac agctgacaag     3060

ggtctttata aactcagtgt ttccctttat cacaatacaa ttccctcctt tgccacttca     3120

tgtcatcata gaatattgtt tttttctcta gcggttcaag gtatgtattt gtatagcagt     3180

cacacctttg ataaaagtta ccatctcttt gattatatat tctcattatg gtaacaaaat     3240

tatattatga ctatttcaat atatctgaaa gtttcattaa attctcatta actttgtata     3300

tttcagtctt gcttattgtg aagcttttat aaattgcttc acttttttct gaaattgtcc     3360

tgttgctaca tcattctgtt aagaaataaa taagtggcaa tattttcccc ctttcttctt     3420

cctctaggct tttaaaatta attttagtat tcatatattt ttatcaaatc aaacatgcaa     3480

ttctctatct gtagagatta acctgtgatc gatacaagtc attttaacta gtgtccttat     3540

gaggctgaca gcaagcataa atcattgatg ttcagcattt atataagctt tcatttatga     3600

gtagagtttg tcagtttata caatagaata taaattataa acaagctatt aataccacaa     3660

aagccttaac attatttagc tgttgcaaac tacatatgaa caactttagt aattttcatg     3720

ttctatttaa gttttatatg cttcaaattt attatttggg gcctcttatt gtttttttat     3780

atggcagcat tctaggatgg agtgtgaaga gatttcttag cctcaatccc catctgatgc     3840

cctatgggca gtgatggctt ctgtttgagg gacactcagt tttctattcg agtcctggta     3900

gagttatcat gttctggtag aaggattcaa gcccagtagt ataggtacac catgccttat     3960

aatgactgag ctattacatt taaaaaagaa taaaagcatg aagctgagga ggcagtgagg     4020

tgggggtgga tctgcggaga gtcacatgga gaagtggagg gcagatttac attatatcaa     4080

attttcaaaa aataataaaa tatttataaa atgtaattga acgaacaata tgtgggttgg     4140

aatcaaaact catgtagaac tttcttcagt cactagatct gattgattgt gaattgatta     4200

ttagccattt aaactagtag actcgagact taccttaatt atttagttgc caaatttggg     4260

atcacaattg aatatgtctc ctcaaaaaat atcttaaaaa ttcatagtaa tcactttcac     4320

tttccttttg cctattttaa aatatatgca ttctctttaa ttaaacagct ctgcatcctg     4380

tttgtcccat aggtaaataa aatgttgagt gtgaatattt agtgaaatat agttcttaat     4440

aaatttgctt tactaaaatt atttttactt tcttagtctt tttttttttt agaaaattaa     4500

aagtaaatgt atagaagtaa aaagtttgtt ttctaaatca gtgttctata tgttgcataa     4560

actggttcct actgctggat tttgttggct ttccagtctc agagcagtgt ctggattaca     4620

ggtagatatt gctgtactag gatgaaagta gactgtttta atgagagata ctttcaattt     4680

cacaggaaag gtgatttcta ttatgttagg ccaataatcc taactttaat ggcatttcat     4740

aatgggtgct gagttaactg tctgatgctg aaattatact aaaaataatg tggtgatcat     4800

ggaacctcaa aataaaacaa aataaactaa aaatttatca ccttttaaaa atctagtatt     4860

atcttttcat taactagaaa acaaatgttg aaatgtttaa aaagaaatat ttagcaatat     4920

acacaataat tgattaattt cagtttaatt tttaaaatat tttattcttt cttaatgaca     4980

actactgtat gatctggctc ataagtagta gcggtagcag cttttgcaga gcatataccc     5040

cctaaaatct gtgtcaatat actatttgag ttccctgaac atctgtttat tacagtgcta     5100

ctcacaacaa ctaggttgtg gagacacccc agatgtctat ccacagacaa atggatagag     5160

aaaccatggc atgtatacat ggtggaggtt tgttcagcca taaagaagaa ttaaattatg     5220

tcatttgcaa gaaaactaat gtatttataa ttcatgatga tgaatgcaat aggtcacact     5280

aaaaatcacg tttttctata ttatatatac atataagtgt atacatgcac attttcagga     5340

ggaactattt gttttttttt taacgcattc tagaatgtag caggaaagac aatattgtgg     5400

gtagtttggg gttgcatcag tataagcaaa gaaaaagtta tacaagtatg aaaattttat     5460

aatgagtaca ttataaaatt tgtaatgtgt acaataaata tataccaata tgctgtataa     5520

taaatatata ccaatgatta aagaaaaaat ccaagtagtt atgtatttta cttataaaga     5580

caaaatgaaa tcagcattaa cttatttcac agatatttcc tagaatttgg gggtataaag     5640

atgattaaga cacataaacc atatgctaca ttgaaaaatg caagtggggt aataggaaag     5700

tagataacta cttataaaat tatgatggaa ataatcattc tagagtaata ttctaaaaca     5760

caatttaaaa agtaaggtca taaaatagaa aaataattac ccatctcaat tctaagacat     5820

aattttttag attagattaa tctcttcttt aacaattata atagaacctt ttcatcattt     5880

atatactgga tgcatgtcta agcactactc tatataattt ctgcttgtca tatgaacact     5940

gagtatggtt atattacttt attttaatga tgaagaagct tgaataacaa agatatttta     6000

agaagtcaaa aaccctatta aattgtatgt atcacaattt tt                        6042


<210>  12
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  20 amino acids at the C-terminus of the aberrant TYR isoform 
       (derived from mouse)

<400>  12

Pro Ile Phe Leu Leu His His Ala Phe Val Asp Arg Ser Arg Leu Leu 
1               5                   10                  15      


Gln Lys Leu Tyr 
            20  


<210>  13
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic immunogenic 9mer in Tyr mouse (derived from mouse) used
       for vaccination

<400>  13

His Ala Phe Val Asp Arg Ser Arg Leu 
1               5                   


<210>  14
<211>  9
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<223>  Synthetic immunogenic 9mer derived from human Tyr

<400>  14

Ala Phe Val Asp Arg Pro Arg Leu Phe 
1               5                   


<210>  15
<211>  8
<212>  PRT
<213>  Gallus gallus


<220>
<221>  MISC_FEATURE
<223>  OVA - 8 amino acids from the ovalbumin protein

<400>  15

Ser Ile Ile Asn Phe Glu Lys Leu 
1               5               


<210>  16
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CRISPR  mTYR 5' guide 1-1 (mouse target)

<400>  16
caccgattgt tcaaaaatac tacag                                             25


<210>  17
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CRISPR mTYR 5' guide 1-2 (mouse target)

<400>  17
aaacctgtag tatttttgaa caatc                                             25


<210>  18
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CRISPR mTYR 3' guide 1-1 (mouse target)

<400>  18
caccgtacag ctacctccaa gagtc                                             25


<210>  19
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CRISPR mTYR 3' guide 1-2 (mouse target)

<400>  19
aaacgactct tggaggtagc tgtac                                             25


<210>  20
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  hTYR 5' guide 1-1, (human target)

<400>  20
caccgtcctc tgcagtattt ttgag                                             25


<210>  21
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  hTYR 5' guide 1-2 (human target)

<400>  21
aaacctcaaa aatactgcag aggac                                             25


<210>  22
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  hTYR 5' guide 2-1 (human target)

<400>  22
caccgactgc tcaaaaatac tgcag                                             25


<210>  23
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  hTYR 5' guide 2-2 (human target)

<400>  23
aaacctgcag tatttttgag cagtc                                             25


<210>  24
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  hTYR 3' guide 3-1, (human target)

<400>  24
caccgtatag ctatctacaa gattc                                             25


<210>  25
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  hTYR 3' guide 3-2, (human target)

<400>  25
aaacgaatct tgtagatagc tatac                                             25


<210>  26
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  hTYR 3' guide 4-1, (human target)

<400>  26
caccgctatc tacaagattc aggta                                             25


<210>  27
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  hTYR 3' guide 4-2, (human target)

<400>  27
aaactacctg aatcttgtag atagc                                             25


<210>  28
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  hTYR oligo 1 (designed for human)

<400>  28
cuuauuuuac uuuaaaaauu                                                   20


<210>  29
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  hTYR oligo 2 (designed for human)

<400>  29
uucaaauguu ucuuuuauac                                                   20


<210>  30
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  hTYR oligo 3 (designed for human)

<400>  30
acaauauguu ucuuagucug                                                   20


<210>  31
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  hTYR oligo 4 (designed for human)

<400>  31
aauaaccuuu uccucugcag                                                   20


<210>  32
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  hTYR oligo 5 (designed for human)

<400>  32
ccuuuuccuc ugcaguauuu                                                   20


<210>  33
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  hTYR oligo 6 (designed for human)

<400>  33
uccucugcag uauuuuugag                                                   20


<210>  34
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  hTYR oligo 7 (designed for human)

<400>  34
ugcaguauuu uugagcagug                                                   20


<210>  35
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  hTYR oligo 8 (designed for human)

<400>  35
uugagcagug gcuccgaagg                                                   20


<210>  36
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  hTYR oligo 9 (designed for human)

<400>  36
gcuccgaagg caccguccuc                                                   20


<210>  37
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  hTYR oligo 10 (designed for human)

<400>  37
caccguccuc uucaagaagu                                                   20


<210>  38
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  hTYR oligo 11 (designed for human)

<400>  38
cauccaaaga ucugggcuau                                                   20


<210>  39
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  hTYR oligo 12 (designed for human)

<400>  39
ucugggcuau gacuauagcu                                                   20


<210>  40
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  hTYR oligo 13 (designed for human)

<400>  40
gacuauagcu aucuacaaga                                                   20


<210>  41
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  hTYR oligo 14 (designed for human)

<400>  41
aucuacaaga uucagguaaa                                                   20


<210>  42
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  hTYR oligo 15 (designed for human)

<400>  42
caagauucag guaaaguuua                                                   20


<210>  43
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  hTYR oligo 16 (designed for human)

<400>  43
uucagguaaa guuuacuuuc                                                   20


<210>  44
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  hTYR oligo 17 (designed for human)

<400>  44
guaaaguuua cuuucuuuca                                                   20


<210>  45
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  hTYR oligo 18 (designed for human)

<400>  45
gaggaauugc ugaaucuagu                                                   20


<210>  46
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  hTYR oligo 19 (designed for human)

<400>  46
guuaccaauu uauuuugaga                                                   20


<210>  47
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  hTYR oligo 20 (designed for human)

<400>  47
uaacacaaaa cuuuaugcuu                                                   20


<210>  48
<211>  103
<212>  DNA
<213>  Mus musculus


<220>
<221>  misc_feature
<223>  Hnrnpab exon 6 in transcript NM_010448.3, mm10_dna 
       range=chr11:51602593-51602695

<400>  48
tgtgaaatca aggttgccca gcccaaagag gtgtatcagc aacagcagta tggctctggg       60

ggcagaggaa atcgcaatcg agggaaccga ggcagtggtg gag                        103


<210>  49
<211>  2404
<212>  DNA
<213>  Mus musculus


<220>
<221>  misc_feature
<223>  Hnrnpab transcript, mm10_ncbiRefSeq_NM_010448.3 
       range=chr11:51600100-51606881

<400>  49
ggcattataa agagctccac gagttggcat tgtcaggcgg tggcaccgcg cgggacggag       60

ttaaggtctt ctttatcggc agtgtacggc ggcggccgag cggagcgata gtcagtggct      120

gcggacgagc cctcgggggt gcgagcgtga ccgcggccaa gcccgcgcgt cgccttctgc      180

ggcgagtagg gcctcgcggc gtcatcggcg gcgagtagcc gctgcggcct ggccgaccat      240

gtcggacgcg gctgaggagc agcccatgga gacgacgggc gccaccgaga acggacacga      300

ggccgccccc gaaggcgagg ccccggtcga gcccagcgcg gctgccgcgg cccccgcggc      360

ctcggccggg tctgggggcg ggaccacgac cgcaccgagc gggaaccaga acggcgcgga      420

gggcgaccag atcaacgcca gcaagaacga ggaggacgcg ggaaaaatgt tcgttggtgg      480

tctgagctgg gataccagca aaaaagactt aaaggattat tttactaagt ttggagaggt      540

tgttgactgt acaataaaaa tggatcccaa cactggacga tcaagagggt ttgggtttat      600

tctcttcaaa gattcttcta gtgtggaaaa ggtcttagat cagaaggagc acaggctgga      660

tggtcgtgtc attgacccta aaaaggctat ggctatgaag aaggaccctg tgaagaaaat      720

ctttgtggga ggtctaaacc ctgaagccac agaggaaaag atcagagaat acttcggcca      780

gtttggggag attgaggcca ttgagcttcc aatagatccc aagttgaaca aaagacgggg      840

ttttgttttt attacattta aagaggaaga tcctgtgaag aaagttctgg agaagaaatt      900

tcatactgtc agtggaagca agtgtgaaat caaggttgcc cagcccaaag aggtgtatca      960

gcaacagcag tatggctctg ggggcagagg aaatcgcaat cgagggaacc gaggcagtgg     1020

tggaggtcag ggtagtacaa attacggaaa gagccagcga cgtggtggtc atcagaataa     1080

ctacaagcca tactgagagg cagcaggagc ggccgagtga cgaccgcaca cgctttgttt     1140

ggacgcggag tgaacacaat tatgtaccaa atttaacttg gcaaactttt atggcctgtc     1200

ccatgtgcat cttatttaaa atttccccca gaaatcactc tcctgtttac tattccagag     1260

ctctagttgt aggcagcgtg tggcgtctca gaggccagag cggcactagg gggctgattt     1320

tattaccagg ttaccctgtt ctagatcagg agggtctatt tcctgttgcc gctctgcagc     1380

ctggacctgt ggaccctggt tgtaaagagt aaaactgtat cttaggaaac cagtgtcacc     1440

tttttcacct tttaatttta tattatttgt gtcatacatt tccagtaatg gaagtgttaa     1500

ttttactgta ctttttggta ccttttggga atctaatgta ttgtaaggta ttttacacgt     1560

gtcctgattt tgccacgacc tggatattga agctatccaa gcttttgaaa taaaaatgaa     1620

aaacaaaacc cccaagcctg ggtaaatgtg ggatgctctg tgagaccttg cccagtgttg     1680

gagatgccag gtctcctgta gcaaagcaga ggtcctttag ttatcccatc tgatcttttt     1740

agctgccctt ttgtgcccat tgtctccata gcagagacca tcttgccatg tattatatat     1800

attccatgtt cttagtttca gtcaagtaca agttgaaatg ttggggatct gctgatggag     1860

gtggggccaa gtctggctta cctggggggt gcttgatcct cagggtttca caacttctta     1920

ctttttcttc catgtctgga aaatgatcac agatcgtttc tttttccccc caagacaggg     1980

tttctctgta gccctggtta ggttatcctg gaactctgta gaccaggctg gccttgaact     2040

cagaaatcca cctggctctg cctcccaagt gctgggatta aaggcgtgcg ccaccacgcc     2100

tggcctaccc tcagtttcta agatgcttgg gatagtttag agattggctt atgtctcatc     2160

atttccctag cacagatggt ccctttgtaa gcccagtcac cactcactgt tctgtacaca     2220

tagaaccctc taagaaatgt atcaattcca aaacgcagta ggggtggcta acaagcagga     2280

ataggaaggg atccttggta gcttgttttt tgtctgttgg gatctgagct ttgtataaca     2340

accactttgg tgtatagtca tttctagttc ggtagttttc ttaaataaat tgagggagac     2400

cttg                                                                  2404


<210>  50
<211>  118
<212>  DNA
<213>  Homo sapiens


<220>
<221>  misc_feature
<223>  HNRNPAB exon 6 in transcript, hg38_ncbiRefSeq_NM_004499.3_5 
       range=chr5:178209330-178209447

<400>  50
tgtgagatca aggtggccca gcccaaagaa gtctatcagc agcagcagta tggctctggg       60

ggccgtggaa accgcaaccg agggaaccga ggcagcggag gtggtggtgg aggtggag        118


<210>  51
<211>  1675
<212>  DNA
<213>  Homo sapiens


<220>
<221>  misc_feature
<223>  HNRNPAB transcript, hg38_ncbiRefSeq_NM_004499.3 
       chr5:178204507-178211183

<400>  51
aaagggcgcc acgagtcggc attgtcaggc ggcggcaccg cgcgggacgg agcttggctg       60

ttggtcggtg ggttcccgtg cggcggcggc caaggaggag gagacacagt tggagcagct      120

ccgtgggctg actggggcga ggcctcagca gcgcgagctt gagtgcggcc gagcctgcgg      180

cgccttcccc tgcgggtggg gacgagcggg ccccgcggcg tcatcggcgg cgaggagccg      240

ccgcgcctcg gcctagcatg tcggaagcgg gcgaggagca gcccatggag acgacgggcg      300

ccaccgagaa cggacatgag gccgtccccg aaggcgagtc gccggccggg gctggcacgg      360

gcgccgcggc gggggctgga ggcgcgaccg cggcgccccc gagcgggaat cagaacggcg      420

ccgagggcga ccagatcaac gccagcaaga acgaggagga cgcgggaaaa atgttcgttg      480

gtggcctgag ctgggatact agcaaaaaag atttaaaaga ctattttact aaatttggag      540

aggtcgttga ctgtacaata aaaatggatc ccaacactgg acggtcaaga gggtttgggt      600

ttatcctgtt caaagatgca gccagtgtgg agaaggtcct agaccagaag gagcacaggc      660

tggatggccg tgtcattgac cctaaaaagg ccatggctat gaagaaggac ccggtgaaga      720

aaatcttcgt tgggggtctg aatcctgaag ccactgagga aaagatcagg gagtactttg      780

gcgagtttgg ggagattgag gccattgaat tgccaatgga tccaaagttg aacaaaagac      840

gaggttttgt gtttatcacc tttaaagaag aagaacccgt gaagaaggtt ctggagaaaa      900

agttccatac tgtcagtgga agcaagtgtg agatcaaggt ggcccagccc aaagaagtct      960

atcagcagca gcagtatggc tctgggggcc gtggaaaccg caaccgaggg aaccgaggca     1020

gcggaggtgg tggtggaggt ggaggtcagg gtagtacaaa ctacggcaag agccagcgac     1080

gtggtggcca tcagaataac tacaagccat actgaggcgg cagcaggagc gaccaactga     1140

tcgcacacat gctttgtttg gatatggagt gaacacaatt atgtaccaaa tttaacttgg     1200

caaactttct attgcctgtc ccatgtgcat cttatttaaa atttccccca tggaaatcac     1260

tctcctgttg actatttcca gagctctagg tgtttaggca gcgtgtggtg tctgagaggc     1320

catagcgcca tcatgggctg atttttatta ccaggtcccc cagaagcagg tgggaggctc     1380

tgcttcctgc tgccgctctg cagcctggac ctgtggaccc tggttgtaaa gagtaaattg     1440

tatcttagga aaccagtgtc accttttttt caccttttaa ttttatatta tttgcgtcat     1500

acatttcctg taacggaagt gttaatttta ctgtactttt tggtaccttt tgggaatcta     1560

atgtattgta aggtatttta cacgtgtcct gattttgcca caacctggat attgaagcta     1620

tccaagcttt tgaaataaaa tttaaaaacc cccaagcctg ggtgagtgtg ggata          1675


<210>  52
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Immunogenic 9mer 1 of aberrant Hnrnpab isoform (derived from 
       mouse)

<400>  52

Val Pro Asn Leu Thr Trp Gln Thr Phe 
1               5                   


<210>  53
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Immunogenic 9mer 2 of aberrant Hnrnpab isoform (derived from 
       mouse)

<400>  53

Val Tyr Tyr Ser Arg Ala Leu Val Val 
1               5                   


<210>  54
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Immunogenic peptide in Balb/c mice (Viral)

<400>  54

Arg Gly Pro Gly Arg Ala Phe Val Thr Ile 
1               5                   10  


<210>  55
<211>  356
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  New protein excluding exon 6 from Hnrnpab transcript NM_010448.3,
       (derived from mouse)

<400>  55

Met Ser Asp Ala Ala Glu Glu Gln Pro Met Glu Thr Thr Gly Ala Thr 
1               5                   10                  15      


Glu Asn Gly His Glu Ala Ala Pro Glu Gly Glu Ala Pro Val Glu Pro 
            20                  25                  30          


Ser Ala Ala Ala Ala Ala Pro Ala Ala Ser Ala Gly Ser Gly Gly Gly 
        35                  40                  45              


Thr Thr Thr Ala Pro Ser Gly Asn Gln Asn Gly Ala Glu Gly Asp Gln 
    50                  55                  60                  


Ile Asn Ala Ser Lys Asn Glu Glu Asp Ala Gly Lys Met Phe Val Gly 
65                  70                  75                  80  


Gly Leu Ser Trp Asp Thr Ser Lys Lys Asp Leu Lys Asp Tyr Phe Thr 
                85                  90                  95      


Lys Phe Gly Glu Val Val Asp Cys Thr Ile Lys Met Asp Pro Asn Thr 
            100                 105                 110         


Gly Arg Ser Arg Gly Phe Gly Phe Ile Leu Phe Lys Asp Ser Ser Ser 
        115                 120                 125             


Val Glu Lys Val Leu Asp Gln Lys Glu His Arg Leu Asp Gly Arg Val 
    130                 135                 140                 


Ile Asp Pro Lys Lys Ala Met Ala Met Lys Lys Asp Pro Val Lys Lys 
145                 150                 155                 160 


Ile Phe Val Gly Gly Leu Asn Pro Glu Ala Thr Glu Glu Lys Ile Arg 
                165                 170                 175     


Glu Tyr Phe Gly Gln Phe Gly Glu Ile Glu Ala Ile Glu Leu Pro Ile 
            180                 185                 190         


Asp Pro Lys Leu Asn Lys Arg Arg Gly Phe Val Phe Ile Thr Phe Lys 
        195                 200                 205             


Glu Glu Asp Pro Val Lys Lys Val Leu Glu Lys Lys Phe His Thr Val 
    210                 215                 220                 


Ser Gly Ser Lys Val Arg Val Val Gln Ile Thr Glu Arg Ala Ser Asp 
225                 230                 235                 240 


Val Val Val Ile Arg Ile Thr Thr Ser His Thr Glu Arg Gln Gln Glu 
                245                 250                 255     


Arg Pro Ser Asp Asp Arg Thr Arg Phe Val Trp Thr Arg Ser Glu His 
            260                 265                 270         


Asn Tyr Val Pro Asn Leu Thr Trp Gln Thr Phe Met Ala Cys Pro Met 
        275                 280                 285             


Cys Ile Leu Phe Lys Ile Ser Pro Arg Asn His Ser Pro Val Tyr Tyr 
    290                 295                 300                 


Ser Arg Ala Leu Val Val Gly Ser Val Trp Arg Leu Arg Gly Gln Ser 
305                 310                 315                 320 


Gly Thr Arg Gly Leu Ile Leu Leu Pro Gly Tyr Pro Val Leu Asp Gln 
                325                 330                 335     


Glu Gly Leu Phe Pro Val Ala Ala Leu Gln Pro Gly Pro Val Asp Pro 
            340                 345                 350         


Gly Cys Lys Glu 
        355     


<210>  56
<211>  254
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  New protein excluding exon 6 from Hnrnpab transcript NM_004499.3,
       (derived from HUNAN)

<400>  56

Met Ser Glu Ala Gly Glu Glu Gln Pro Met Glu Thr Thr Gly Ala Thr 
1               5                   10                  15      


Glu Asn Gly His Glu Ala Val Pro Glu Gly Glu Ser Pro Ala Gly Ala 
            20                  25                  30          


Gly Thr Gly Ala Ala Ala Gly Ala Gly Gly Ala Thr Ala Ala Pro Pro 
        35                  40                  45              


Ser Gly Asn Gln Asn Gly Ala Glu Gly Asp Gln Ile Asn Ala Ser Lys 
    50                  55                  60                  


Asn Glu Glu Asp Ala Gly Lys Met Phe Val Gly Gly Leu Ser Trp Asp 
65                  70                  75                  80  


Thr Ser Lys Lys Asp Leu Lys Asp Tyr Phe Thr Lys Phe Gly Glu Val 
                85                  90                  95      


Val Asp Cys Thr Ile Lys Met Asp Pro Asn Thr Gly Arg Ser Arg Gly 
            100                 105                 110         


Phe Gly Phe Ile Leu Phe Lys Asp Ala Ala Ser Val Glu Lys Val Leu 
        115                 120                 125             


Asp Gln Lys Glu His Arg Leu Asp Gly Arg Val Ile Asp Pro Lys Lys 
    130                 135                 140                 


Ala Met Ala Met Lys Lys Asp Pro Val Lys Lys Ile Phe Val Gly Gly 
145                 150                 155                 160 


Leu Asn Pro Glu Ala Thr Glu Glu Lys Ile Arg Glu Tyr Phe Gly Glu 
                165                 170                 175     


Phe Gly Glu Ile Glu Ala Ile Glu Leu Pro Met Asp Pro Lys Leu Asn 
            180                 185                 190         


Lys Arg Arg Gly Phe Val Phe Ile Thr Phe Lys Glu Glu Glu Pro Val 
        195                 200                 205             


Lys Lys Val Leu Glu Lys Lys Phe His Thr Val Ser Gly Ser Lys Val 
    210                 215                 220                 


Arg Val Val Gln Thr Thr Ala Arg Ala Ser Asp Val Val Ala Ile Arg 
225                 230                 235                 240 


Ile Thr Thr Ser His Thr Glu Ala Ala Ala Gly Ala Thr Asn 
                245                 250                 


<210>  57
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CRISPR mHnrnpab 3' guide 1-1 (mouse target)

<400>  57
caccggggaa ccgaggcagt ggtgg                                             25


<210>  58
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CRISPR mHnrnpab 3' guide 1-2 (mouse target)

<400>  58
aaacccacca ctgcctcggt tcccc                                             25


<210>  59
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  hHnrnpab 5' guide 1-1, (human target)

<400>  59
caccgtgact tttagtgtga gatca                                             25


<210>  60
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  hHnrnpab 5' guide 1-2, (human target)

<400>  60
aaactgatct cacactaaaa gtcac                                             25


<210>  61
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  hHnrnpab 5' guide 2-1, (human target)

<400>  61
caccggtcct cttgactttt agtgt                                             25


<210>  62
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  hHnrnpab 5' guide 2-2, (human target)

<400>  62
aaacacacta aaagtcaaga ggacc                                             25


<210>  63
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  hHnrnpab 3' guide 3-1, (human target)

<400>  63
caccgggagg tggtggtgga ggtgg                                             25


<210>  64
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  hHnrnpab 3' guide 3-2, (human target)

<400>  64
aaacccacct ccaccaccac ctccc                                             25


<210>  65
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  hHnrnpab 3' guide 4-1, (human target)

<400>  65
caccggtggt ggaggtggag gtgag                                             25


<210>  66
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  hHnrnpab 3' guide 4-2, (human target)

<400>  66
aaacctcacc tccacctcca ccacc                                             25


<210>  67
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  hHNRNPAB oligo 1 (designed for human)

<400>  67
uuugucgcag ugaagguguu                                                   20


<210>  68
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  hHNRNPAB oligo 2 (designed for human)

<400>  68
ugggcaguca cugcccugag                                                   20


<210>  69
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  hHNRNPAB oligo 3 (designed for human)

<400>  69
uuuguccuac uggccugacc                                                   20


<210>  70
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  hHNRNPAB oligo 4 (designed for human)

<400>  70
acuguccucu ugacuuuuag                                                   20


<210>  71
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  hHNRNPAB oligo 5 (designed for human)

<400>  71
ccucuugacu uuuaguguga                                                   20


<210>  72
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  hHNRNPAB oligo 6 (designed for human)

<400>  72
ugacuuuuag ugugagauca                                                   20


<210>  73
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  hHNRNPAB oligo 7 (designed for human)

<400>  73
uuuaguguga gaucaaggug                                                   20


<210>  74
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  hHNRNPAB oligo 8 (designed for human)

<400>  74
gaucaaggug gcccagccca                                                   20


<210>  75
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  hHNRNPAB oligo 9 (designed for human)

<400>  75
gcccagccca aagaagucua                                                   20


<210>  76
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  hHNRNPAB oligo 10 (designed for human)

<400>  76
aagaagucua ucagcagcag                                                   20


<210>  77
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  hHNRNPAB oligo 11 (designed for human)

<400>  77
gcaaccgagg gaaccgaggc                                                   20


<210>  78
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  hHNRNPAB oligo 12 (designed for human)

<400>  78
gaaccgaggc agcggaggug                                                   20


<210>  79
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  hHNRNPAB oligo 13 (designed for human)

<400>  79
agcggaggug gugguggagg                                                   20


<210>  80
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  hHNRNPAB oligo 14 (designed for human)

<400>  80
gugguggagg uggaggugag                                                   20


<210>  81
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  hHNRNPAB oligo 15 (designed for human)

<400>  81
ggagguggag gugaguggaa                                                   20


<210>  82
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  hHNRNPAB oligo 16 (designed for human)

<400>  82
uggaggugag uggaacgugg                                                   20


<210>  83
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  hHNRNPAB oligo 17 (designed for human)

<400>  83
gugaguggaa cguggaugaa                                                   20


<210>  84
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  hHNRNPAB oligo 18 (designed for human)

<400>  84
gauagguccu guuucccugc                                                   20


<210>  85
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  hHNRNPAB oligo 19 (designed for human)

<400>  85
cuacauggag ccuuccaagc                                                   20


<210>  86
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  hHNRNPAB oligo 20 (designed for human)

<400>  86
cugucuuggg gcucccucug                                                   20


<210>  87
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CRISPR mHnrnpab 5' guide 1-1 (mouse target)

<400>  87
tggcttttag tgtgaaatca                                                   20


<210>  88
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CRISPR mHnrnpab 5' guide 1-2 : (mouse target)

<400>  88
tgatttcaca ctaaaagcca                                                   20


<210>  89
<211>  9
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<223>  Synthetic immunogenic 9mer derived from human Tyr

<400>  89

His Ala Phe Val Asp Arg Pro Arg Leu 
1               5                   


