               SEQUENCE LISTING

<110> IDT Biologika GmbH

<120> Inactivated ApxIA, ApxIIA and ApxIIIA toxins

<130> P46559-WO

<150> 19154466.7
<151> 2019-01-30

<160> 34

<170> BiSSAP 1.3.6

<210> 1
<211> 1022
<212> PRT
<213> Actonbacillus pleuropneumoniae


<220> 
<223> ApxIA wild type

<400> 1
Met Ala Asn Ser Gln Leu Asp Arg Val Lys Gly Leu Ile Asp Ser Leu 
1               5                   10                  15      
Asn Gln His Thr Lys Ser Ala Ala Lys Ser Gly Ala Gly Ala Leu Lys 
            20                  25                  30          
Asn Gly Leu Gly Gln Val Lys Gln Ala Gly Gln Lys Leu Ile Leu Tyr 
        35                  40                  45              
Ile Pro Lys Asp Tyr Gln Ala Ser Thr Gly Ser Ser Leu Asn Asp Leu 
    50                  55                  60                  
Val Lys Ala Ala Glu Ala Leu Gly Ile Glu Val His Arg Ser Glu Lys 
65                  70                  75                  80  
Asn Gly Thr Ala Leu Ala Lys Glu Leu Phe Gly Thr Thr Glu Lys Leu 
                85                  90                  95      
Leu Gly Phe Ser Glu Arg Gly Ile Ala Leu Phe Ala Pro Gln Phe Asp 
            100                 105                 110         
Lys Leu Leu Asn Lys Asn Gln Lys Leu Ser Lys Ser Leu Gly Gly Ser 
        115                 120                 125             
Ser Glu Ala Leu Gly Gln Arg Leu Asn Lys Thr Gln Thr Ala Leu Ser 
    130                 135                 140                 
Ala Leu Gln Ser Phe Leu Gly Thr Ala Ile Ala Gly Met Asp Leu Asp 
145                 150                 155                 160 
Ser Leu Leu Arg Arg Arg Arg Asn Gly Glu Asp Val Ser Gly Ser Glu 
                165                 170                 175     
Leu Ala Lys Ala Gly Val Asp Leu Ala Ala Gln Leu Val Asp Asn Ile 
            180                 185                 190         
Ala Ser Ala Thr Gly Thr Val Asp Ala Phe Ala Glu Gln Leu Gly Lys 
        195                 200                 205             
Leu Gly Asn Ala Leu Ser Asn Thr Arg Leu Ser Gly Leu Ala Ser Lys 
    210                 215                 220                 
Leu Asn Asn Leu Pro Asp Leu Ser Leu Ala Gly Pro Gly Phe Asp Ala 
225                 230                 235                 240 
Val Ser Gly Ile Leu Ser Val Val Ser Ala Ser Phe Ile Leu Ser Asn 
                245                 250                 255     
Lys Asp Ala Asp Ala Gly Thr Lys Ala Ala Ala Gly Ile Glu Ile Ser 
            260                 265                 270         
Thr Lys Ile Leu Gly Asn Ile Gly Lys Ala Val Ser Gln Tyr Ile Ile 
        275                 280                 285             
Ala Gln Arg Val Ala Ala Gly Leu Ser Thr Thr Ala Ala Thr Gly Gly 
    290                 295                 300                 
Leu Ile Gly Ser Val Val Ala Leu Ala Ile Ser Pro Leu Ser Phe Leu 
305                 310                 315                 320 
Asn Val Ala Asp Lys Phe Glu Arg Ala Lys Gln Leu Glu Gln Tyr Ser 
                325                 330                 335     
Glu Arg Phe Lys Lys Phe Gly Tyr Glu Gly Asp Ser Leu Leu Ala Ser 
            340                 345                 350         
Phe Tyr Arg Glu Thr Gly Ala Ile Glu Ala Ala Leu Thr Thr Ile Asn 
        355                 360                 365             
Ser Val Leu Ser Ala Ala Ser Ala Gly Val Gly Ala Ala Ala Thr Gly 
    370                 375                 380                 
Ser Leu Val Gly Ala Pro Val Ala Ala Leu Val Ser Ala Ile Thr Gly 
385                 390                 395                 400 
Ile Ile Ser Gly Ile Leu Asp Ala Ser Lys Gln Ala Ile Phe Glu Arg 
                405                 410                 415     
Val Ala Thr Lys Leu Ala Asn Lys Ile Asp Glu Trp Glu Lys Lys His 
            420                 425                 430         
Gly Lys Asn Tyr Phe Glu Asn Gly Tyr Asp Ala Arg His Ser Ala Phe 
        435                 440                 445             
Leu Glu Asp Thr Phe Glu Leu Leu Ser Gln Tyr Asn Lys Glu Tyr Ser 
    450                 455                 460                 
Val Glu Arg Val Val Ala Ile Thr Gln Gln Arg Trp Asp Val Asn Ile 
465                 470                 475                 480 
Gly Glu Leu Ala Gly Ile Thr Arg Lys Gly Ala Asp Ala Lys Ser Gly 
                485                 490                 495     
Lys Ala Tyr Val Asp Phe Phe Glu Glu Gly Lys Leu Leu Glu Lys Asp 
            500                 505                 510         
Pro Asp Arg Phe Asp Lys Lys Val Phe Asp Pro Leu Glu Gly Lys Ile 
        515                 520                 525             
Asp Leu Ser Ser Ile Asn Lys Thr Thr Leu Leu Lys Phe Ile Thr Pro 
    530                 535                 540                 
Val Phe Thr Ala Gly Glu Glu Ile Arg Glu Arg Lys Gln Thr Gly Lys 
545                 550                 555                 560 
Tyr Glu Tyr Met Thr Glu Leu Phe Val Lys Gly Lys Glu Lys Trp Val 
                565                 570                 575     
Val Thr Gly Val Gln Ser His Asn Ala Ile Tyr Asp Tyr Thr Asn Leu 
            580                 585                 590         
Ile Gln Leu Ala Ile Asp Lys Lys Gly Glu Lys Arg Gln Val Thr Ile 
        595                 600                 605             
Glu Ser His Leu Gly Glu Lys Asn Asp Arg Ile Tyr Leu Ser Ser Gly 
    610                 615                 620                 
Ser Ser Ile Val Tyr Ala Gly Asn Gly His Asp Val Ala Tyr Tyr Asp 
625                 630                 635                 640 
Lys Thr Asp Thr Gly Tyr Leu Thr Phe Asp Gly Gln Ser Ala Gln Lys 
                645                 650                 655     
Ala Gly Glu Tyr Ile Val Thr Lys Glu Leu Lys Ala Asp Val Lys Val 
            660                 665                 670         
Leu Lys Glu Val Val Lys Thr Gln Asp Ile Ser Val Gly Lys Arg Ser 
        675                 680                 685             
Glu Lys Leu Glu Tyr Arg Asp Tyr Glu Leu Ser Pro Phe Glu Leu Gly 
    690                 695                 700                 
Asn Gly Ile Arg Ala Lys Asp Glu Leu His Ser Val Glu Glu Ile Ile 
705                 710                 715                 720 
Gly Ser Asn Arg Lys Asp Lys Phe Phe Gly Ser Arg Phe Thr Asp Ile 
                725                 730                 735     
Phe His Gly Ala Lys Gly Asp Asp Glu Ile Tyr Gly Asn Asp Gly His 
            740                 745                 750         
Asp Ile Leu Tyr Gly Asp Asp Gly Asn Asp Val Ile His Gly Gly Asp 
        755                 760                 765             
Gly Asn Asp His Leu Val Gly Gly Asn Gly Asn Asp Arg Leu Ile Gly 
    770                 775                 780                 
Gly Lys Gly Asn Asn Phe Leu Asn Gly Gly Asp Gly Asp Asp Glu Leu 
785                 790                 795                 800 
Gln Val Phe Glu Gly Gln Tyr Asn Val Leu Leu Gly Gly Ala Gly Asn 
                805                 810                 815     
Asp Ile Leu Tyr Gly Ser Asp Gly Thr Asn Leu Phe Asp Gly Gly Val 
            820                 825                 830         
Gly Asn Asp Lys Ile Tyr Gly Gly Leu Gly Lys Asp Ile Tyr Arg Tyr 
        835                 840                 845             
Ser Lys Glu Tyr Gly Arg His Ile Ile Ile Glu Lys Gly Gly Asp Asp 
    850                 855                 860                 
Asp Thr Leu Leu Leu Ser Asp Leu Ser Phe Lys Asp Val Gly Phe Ile 
865                 870                 875                 880 
Arg Ile Gly Asp Asp Leu Leu Val Asn Lys Arg Ile Gly Gly Thr Leu 
                885                 890                 895     
Tyr Tyr His Glu Asp Tyr Asn Gly Asn Ala Leu Thr Ile Lys Asp Trp 
            900                 905                 910         
Phe Lys Glu Gly Lys Glu Gly Gln Asn Asn Lys Ile Glu Lys Ile Val 
        915                 920                 925             
Asp Lys Asp Gly Ala Tyr Val Leu Ser Gln Tyr Leu Thr Glu Leu Thr 
    930                 935                 940                 
Ala Pro Gly Arg Gly Ile Asn Tyr Phe Asn Gly Leu Glu Glu Lys Leu 
945                 950                 955                 960 
Tyr Tyr Gly Glu Gly Tyr Asn Ala Leu Pro Gln Leu Arg Lys Asp Ile 
                965                 970                 975     
Glu Gln Ile Ile Ser Ser Thr Gly Ala Leu Thr Gly Glu His Gly Gln 
            980                 985                 990         
Val Leu Val Gly Ala Gly Gly Pro Leu Ala Tyr Ser Asn Ser Pro Asn 
        995                 1000                1005            
Ser Ile Pro Asn Ala Phe Ser Asn Tyr Leu Thr Gln Ser Ala 
    1010                1015                1020        

<210> 2
<211> 956
<212> PRT
<213> Actonbacillus pleuropneumoniae


<220> 
<223> ApxIIA wild type

<400> 2
Met Ser Lys Ile Thr Leu Ser Ser Leu Lys Ser Ser Leu Gln Gln Gly 
1               5                   10                  15      
Leu Lys Asn Gly Lys Asn Lys Leu Asn Gln Ala Gly Thr Thr Leu Lys 
            20                  25                  30          
Asn Gly Leu Thr Gln Thr Gly His Ser Leu Gln Asn Gly Ala Lys Lys 
        35                  40                  45              
Leu Ile Leu Tyr Ile Pro Gln Gly Tyr Asp Ser Gly Gln Gly Asn Gly 
    50                  55                  60                  
Val Gln Asp Leu Val Lys Ala Ala Asn Asp Leu Gly Ile Glu Val Trp 
65                  70                  75                  80  
Arg Glu Glu Arg Ser Asn Leu Asp Ile Ala Lys Thr Ser Phe Asp Thr 
                85                  90                  95      
Thr Gln Lys Ile Leu Gly Phe Thr Asp Arg Gly Ile Val Leu Phe Ala 
            100                 105                 110         
Pro Gln Leu Asp Asn Leu Leu Lys Lys Asn Pro Lys Ile Gly Asn Thr 
        115                 120                 125             
Leu Gly Ser Ala Ser Ser Ile Ser Gln Asn Ile Gly Lys Ala Asn Thr 
    130                 135                 140                 
Val Leu Gly Gly Ile Gln Ser Ile Leu Gly Ser Val Leu Ser Gly Val 
145                 150                 155                 160 
Asn Leu Asn Glu Leu Leu Gln Asn Lys Asp Pro Asn Gln Leu Glu Leu 
                165                 170                 175     
Ala Lys Ala Gly Leu Glu Leu Thr Asn Glu Leu Val Gly Asn Ile Ala 
            180                 185                 190         
Ser Ser Val Gln Thr Val Asp Ala Phe Ala Glu Gln Ile Ser Lys Leu 
        195                 200                 205             
Gly Ser His Leu Gln Asn Val Lys Gly Leu Gly Gly Leu Ser Asn Lys 
    210                 215                 220                 
Leu Gln Asn Leu Pro Asp Leu Gly Lys Ala Ser Leu Gly Leu Asp Ile 
225                 230                 235                 240 
Ile Ser Gly Leu Leu Ser Gly Ala Ser Ala Gly Leu Ile Leu Ala Asp 
                245                 250                 255     
Lys Glu Ala Ser Thr Glu Lys Lys Ala Ala Ala Gly Val Glu Phe Ala 
            260                 265                 270         
Asn Gln Ile Ile Gly Asn Val Thr Lys Ala Val Ser Ser Tyr Ile Leu 
        275                 280                 285             
Ala Gln Arg Val Ala Ser Gly Leu Ser Ser Thr Gly Pro Val Ala Ala 
    290                 295                 300                 
Leu Ile Ala Ser Thr Val Ala Leu Ala Val Ser Pro Leu Ser Phe Leu 
305                 310                 315                 320 
Asn Val Ala Asp Lys Phe Lys Gln Ala Asp Leu Ile Lys Ser Tyr Ser 
                325                 330                 335     
Glu Arg Phe Gln Lys Leu Gly Tyr Asp Gly Asp Arg Leu Leu Ala Asp 
            340                 345                 350         
Phe His Arg Glu Thr Gly Thr Ile Asp Ala Ser Val Thr Thr Ile Asn 
        355                 360                 365             
Thr Ala Leu Ala Ala Ile Ser Gly Gly Val Gly Ala Ala Ser Ala Gly 
    370                 375                 380                 
Ser Leu Val Gly Ala Pro Val Ala Leu Leu Val Ala Gly Val Thr Gly 
385                 390                 395                 400 
Leu Ile Thr Thr Ile Leu Glu Tyr Ser Lys Gln Ala Met Phe Glu His 
                405                 410                 415     
Val Ala Asn Lys Val His Asp Arg Ile Val Glu Trp Glu Lys Lys His 
            420                 425                 430         
Asn Lys Asn Tyr Phe Glu Gln Gly Tyr Asp Ser Arg His Leu Ala Asp 
        435                 440                 445             
Leu Gln Asp Asn Met Lys Phe Leu Ile Asn Leu Asn Lys Glu Leu Gln 
    450                 455                 460                 
Ala Glu Arg Val Val Ala Ile Thr Gln Gln Arg Trp Asp Asn Gln Ile 
465                 470                 475                 480 
Gly Asp Leu Ala Ala Ile Ser Arg Arg Thr Asp Lys Ile Ser Ser Gly 
                485                 490                 495     
Lys Ala Tyr Val Asp Ala Phe Glu Glu Gly Gln His Gln Ser Tyr Asp 
            500                 505                 510         
Ser Ser Val Gln Leu Asp Asn Lys Asn Gly Ile Ile Asn Ile Ser Asn 
        515                 520                 525             
Thr Asn Arg Lys Thr Gln Ser Val Leu Phe Arg Thr Pro Leu Leu Thr 
    530                 535                 540                 
Pro Gly Glu Glu Asn Arg Glu Arg Ile Gln Glu Gly Lys Asn Ser Tyr 
545                 550                 555                 560 
Ile Thr Lys Leu His Ile Gln Arg Val Asp Ser Trp Thr Val Thr Asp 
                565                 570                 575     
Gly Asp Ala Ser Ser Ser Val Asp Phe Thr Asn Val Val Gln Arg Ile 
            580                 585                 590         
Ala Val Lys Phe Asp Asp Ala Gly Asn Ile Ile Glu Ser Lys Asp Thr 
        595                 600                 605             
Lys Ile Ile Ala Asn Leu Gly Ala Gly Asn Asp Asn Val Phe Val Gly 
    610                 615                 620                 
Ser Ser Thr Thr Val Ile Asp Gly Gly Asp Gly His Asp Arg Val His 
625                 630                 635                 640 
Tyr Ser Arg Gly Glu Tyr Gly Ala Leu Val Ile Asp Ala Thr Ala Glu 
                645                 650                 655     
Thr Glu Lys Gly Ser Tyr Ser Val Lys Arg Tyr Val Gly Asp Ser Lys 
            660                 665                 670         
Ala Leu His Glu Thr Ile Ala Thr His Gln Thr Asn Val Gly Asn Arg 
        675                 680                 685             
Glu Glu Lys Ile Glu Tyr Arg Arg Glu Asp Asp Arg Phe His Thr Gly 
    690                 695                 700                 
Tyr Thr Val Thr Asp Ser Leu Lys Ser Val Glu Glu Ile Ile Gly Ser 
705                 710                 715                 720 
Gln Phe Asn Asp Ile Phe Lys Gly Ser Gln Phe Asp Asp Val Phe His 
                725                 730                 735     
Gly Gly Asn Gly Val Asp Thr Ile Asp Gly Asn Asp Gly Asp Asp His 
            740                 745                 750         
Leu Phe Gly Gly Ala Gly Asp Asp Val Ile Asp Gly Gly Asn Gly Asn 
        755                 760                 765             
Asn Phe Leu Val Gly Gly Thr Gly Asn Asp Ile Ile Ser Gly Gly Lys 
    770                 775                 780                 
Asp Asn Asp Ile Tyr Val His Lys Thr Gly Asp Gly Asn Asp Ser Ile 
785                 790                 795                 800 
Thr Asp Ser Gly Gly Gln Asp Lys Leu Ala Phe Ser Asp Val Asn Leu 
                805                 810                 815     
Lys Asp Leu Thr Phe Lys Lys Val Asp Ser Ser Leu Glu Ile Ile Asn 
            820                 825                 830         
Gln Lys Gly Glu Lys Val Arg Ile Gly Asn Trp Phe Leu Glu Asp Asp 
        835                 840                 845             
Leu Ala Ser Thr Val Ala Asn Tyr Lys Ala Thr Asn Asp Arg Lys Ile 
    850                 855                 860                 
Glu Glu Ile Ile Gly Lys Gly Gly Glu Arg Ile Thr Ser Glu Gln Val 
865                 870                 875                 880 
Asp Lys Leu Ile Lys Glu Gly Asn Asn Gln Ile Ser Ala Glu Ala Leu 
                885                 890                 895     
Ser Lys Val Val Asn Asp Tyr Asn Thr Ser Lys Asp Arg Gln Asn Val 
            900                 905                 910         
Ser Asn Ser Leu Ala Lys Leu Ile Ser Ser Val Gly Ser Phe Thr Ser 
        915                 920                 925             
Ser Ser Asp Phe Arg Asn Asn Leu Gly Thr Tyr Val Pro Ser Ser Ile 
    930                 935                 940                 
Asp Val Ser Asn Asn Ile Gln Leu Ala Arg Ala Ala 
945                 950                 955     

<210> 3
<211> 1052
<212> PRT
<213> Actonbacillus pleuropneumoniae


<220> 
<223> ApxIIIA wild type

<400> 3
Met Ser Thr Trp Ser Ser Met Leu Ala Asp Leu Lys Lys Arg Ala Glu 
1               5                   10                  15      
Glu Ala Lys Arg Gln Ala Lys Lys Gly Tyr Asp Val Thr Lys Asn Gly 
            20                  25                  30          
Leu Gln Tyr Gly Val Ser Gln Ala Lys Leu Gln Ala Leu Ala Ala Gly 
        35                  40                  45              
Lys Ala Val Gln Lys Tyr Gly Asn Lys Leu Val Leu Val Ile Pro Lys 
    50                  55                  60                  
Glu Tyr Asp Gly Ser Val Gly Asn Gly Phe Phe Asp Leu Val Lys Ala 
65                  70                  75                  80  
Ala Glu Glu Leu Gly Ile Gln Val Lys Tyr Val Asn Arg Asn Glu Leu 
                85                  90                  95      
Glu Val Ala His Lys Ser Leu Gly Thr Ala Asp Gln Phe Leu Gly Leu 
            100                 105                 110         
Thr Glu Arg Gly Leu Thr Leu Phe Ala Pro Gln Leu Asp Gln Phe Leu 
        115                 120                 125             
Gln Lys His Ser Lys Ile Ser Asn Val Val Gly Ser Ser Thr Gly Asp 
    130                 135                 140                 
Ala Val Ser Lys Leu Ala Lys Ser Gln Thr Ile Ile Ser Gly Ile Gln 
145                 150                 155                 160 
Ser Val Leu Gly Thr Val Leu Ala Gly Ile Asn Leu Asn Glu Ala Ile 
                165                 170                 175     
Ile Ser Gly Gly Ser Glu Leu Glu Leu Ala Glu Ala Gly Val Ser Leu 
            180                 185                 190         
Ala Ser Glu Leu Val Ser Asn Ile Ala Lys Gly Thr Thr Thr Ile Asp 
        195                 200                 205             
Ala Phe Thr Thr Gln Ile Gln Asn Phe Gly Lys Leu Val Glu Asn Ala 
    210                 215                 220                 
Lys Gly Leu Gly Gly Val Gly Arg Gln Leu Gln Asn Ile Ser Gly Ser 
225                 230                 235                 240 
Ala Leu Ser Lys Thr Gly Leu Gly Leu Asp Ile Ile Ser Ser Leu Leu 
                245                 250                 255     
Ser Gly Val Thr Ala Ser Phe Ala Leu Ala Asn Lys Asn Ala Ser Thr 
            260                 265                 270         
Ser Thr Lys Val Ala Ala Gly Phe Glu Leu Ser Asn Gln Val Ile Gly 
        275                 280                 285             
Gly Ile Thr Lys Ala Val Ser Ser Tyr Ile Leu Ala Gln Arg Leu Ala 
    290                 295                 300                 
Ala Gly Leu Ser Thr Thr Gly Pro Ala Ala Ala Leu Ile Ala Ser Ser 
305                 310                 315                 320 
Ile Ser Leu Ala Ile Ser Pro Leu Ala Phe Leu Arg Val Ala Asp Asn 
                325                 330                 335     
Phe Asn Arg Ser Lys Glu Ile Gly Glu Phe Ala Glu Arg Phe Lys Lys 
            340                 345                 350         
Leu Gly Tyr Asp Gly Asp Lys Leu Leu Ser Glu Phe Tyr His Glu Ala 
        355                 360                 365             
Gly Thr Ile Asp Ala Ser Ile Thr Thr Ile Ser Thr Ala Leu Ser Ala 
    370                 375                 380                 
Ile Ala Ala Gly Thr Ala Ala Ala Ser Ala Gly Ala Leu Val Gly Ala 
385                 390                 395                 400 
Pro Ile Thr Leu Leu Val Thr Gly Ile Thr Gly Leu Ile Ser Gly Ile 
                405                 410                 415     
Leu Glu Phe Ser Lys Gln Pro Met Leu Asp His Val Ala Ser Lys Ile 
            420                 425                 430         
Gly Asn Lys Ile Asp Glu Trp Glu Lys Lys Tyr Gly Lys Asn Tyr Phe 
        435                 440                 445             
Glu Asn Gly Tyr Asp Ala Arg His Lys Ala Phe Leu Glu Asp Ser Phe 
    450                 455                 460                 
Ser Leu Leu Ser Ser Phe Asn Lys Gln Tyr Glu Thr Glu Arg Ala Val 
465                 470                 475                 480 
Leu Ile Thr Gln Gln Arg Trp Asp Glu Tyr Ile Gly Glu Leu Ala Gly 
                485                 490                 495     
Ile Thr Gly Lys Gly Asp Lys Leu Ser Ser Gly Lys Ala Tyr Val Asp 
            500                 505                 510         
Tyr Phe Gln Glu Gly Lys Leu Leu Glu Lys Lys Pro Asp Asp Phe Ser 
        515                 520                 525             
Lys Val Val Phe Asp Pro Thr Lys Gly Glu Ile Asp Ile Ser Asn Ser 
    530                 535                 540                 
Gln Thr Ser Thr Leu Leu Lys Phe Val Thr Pro Leu Leu Thr Pro Gly 
545                 550                 555                 560 
Thr Glu Ser Arg Glu Arg Thr Gln Thr Gly Lys Tyr Glu Tyr Ile Thr 
                565                 570                 575     
Lys Leu Val Val Lys Gly Lys Asp Lys Trp Val Val Asn Gly Val Lys 
            580                 585                 590         
Asp Lys Gly Ala Val Tyr Asp Tyr Thr Asn Leu Ile Gln His Ala His 
        595                 600                 605             
Ile Ser Ser Ser Val Ala Arg Gly Glu Glu Tyr Arg Glu Val Arg Leu 
    610                 615                 620                 
Val Ser His Leu Gly Asn Gly Asn Asp Lys Val Phe Leu Ala Ala Gly 
625                 630                 635                 640 
Ser Ala Glu Ile His Ala Gly Glu Gly His Asp Val Val Tyr Tyr Asp 
                645                 650                 655     
Lys Thr Asp Thr Gly Leu Leu Val Ile Asp Gly Thr Lys Ala Thr Glu 
            660                 665                 670         
Gln Gly Arg Tyr Ser Val Thr Arg Glu Leu Ser Gly Ala Thr Lys Ile 
        675                 680                 685             
Leu Arg Glu Val Ile Lys Asn Gln Lys Ser Ala Val Gly Lys Arg Glu 
    690                 695                 700                 
Glu Thr Leu Glu Tyr Arg Asp Tyr Glu Leu Thr Gln Ser Gly Asn Ser 
705                 710                 715                 720 
Asn Leu Lys Ala His Asp Glu Leu His Ser Val Glu Glu Ile Ile Gly 
                725                 730                 735     
Ser Asn Gln Arg Asp Glu Phe Lys Gly Ser Lys Phe Arg Asp Ile Phe 
            740                 745                 750         
His Gly Ala Asp Gly Asp Asp Leu Leu Asn Gly Asn Asp Gly Asp Asp 
        755                 760                 765             
Ile Leu Tyr Gly Asp Lys Gly Asn Asp Glu Leu Arg Gly Asp Asn Gly 
    770                 775                 780                 
Asn Asp Gln Leu Tyr Gly Gly Glu Gly Asn Asp Lys Leu Leu Gly Gly 
785                 790                 795                 800 
Asn Gly Asn Asn Tyr Leu Ser Gly Gly Asp Gly Asn Asp Glu Leu Gln 
                805                 810                 815     
Val Leu Gly Asn Gly Phe Asn Val Leu Arg Gly Gly Lys Gly Asp Asp 
            820                 825                 830         
Lys Leu Tyr Gly Ser Ser Gly Ser Asp Leu Leu Asp Gly Gly Glu Gly 
        835                 840                 845             
Asn Asp Tyr Leu Glu Gly Gly Asp Gly Ser Asp Phe Tyr Val Tyr Arg 
    850                 855                 860                 
Ser Thr Ser Gly Asn His Thr Ile Tyr Asp Gln Gly Lys Ser Ser Asp 
865                 870                 875                 880 
Leu Asp Lys Leu Tyr Leu Ser Asp Phe Ser Phe Asp Arg Leu Leu Val 
                885                 890                 895     
Glu Lys Val Asp Asp Asn Leu Val Leu Arg Ser Asn Glu Ser Ser His 
            900                 905                 910         
Asn Asn Gly Val Leu Thr Ile Lys Asp Trp Phe Lys Glu Gly Asn Lys 
        915                 920                 925             
Tyr Asn His Lys Ile Glu Gln Ile Val Asp Lys Asn Gly Arg Lys Leu 
    930                 935                 940                 
Thr Ala Glu Asn Leu Gly Thr Tyr Phe Lys Asn Ala Pro Lys Ala Asp 
945                 950                 955                 960 
Asn Leu Leu Asn Tyr Ala Thr Lys Glu Asp Gln Asn Glu Ser Asn Leu 
                965                 970                 975     
Ser Ser Leu Lys Thr Glu Leu Ser Lys Ile Ile Thr Asn Ala Gly Asn 
            980                 985                 990         
Phe Gly Val Ala Lys Gln Gly Asn Thr Gly Ile Asn Thr Ala Ala Leu 
        995                 1000                1005            
Asn Asn Glu Val Asn Lys Ile Ile Ser Ser Ala Asn Thr Phe Ala Thr 
    1010                1015                1020                
Ser Gln Leu Gly Gly Ser Gly Met Gly Thr Leu Pro Ser Thr Asn Val 
1025                1030                1035                1040
Asn Ser Met Met Leu Gly Asn Leu Ala Arg Ala Ala 
                1045                1050        

<210> 4
<211> 1022
<212> PRT
<213> Actonbacillus pleuropneumoniae


<220> 
<223> ApxIA K560A K686A

<400> 4
Met Ala Asn Ser Gln Leu Asp Arg Val Lys Gly Leu Ile Asp Ser Leu 
1               5                   10                  15      
Asn Gln His Thr Lys Ser Ala Ala Lys Ser Gly Ala Gly Ala Leu Lys 
            20                  25                  30          
Asn Gly Leu Gly Gln Val Lys Gln Ala Gly Gln Lys Leu Ile Leu Tyr 
        35                  40                  45              
Ile Pro Lys Asp Tyr Gln Ala Ser Thr Gly Ser Ser Leu Asn Asp Leu 
    50                  55                  60                  
Val Lys Ala Ala Glu Ala Leu Gly Ile Glu Val His Arg Ser Glu Lys 
65                  70                  75                  80  
Asn Gly Thr Ala Leu Ala Lys Glu Leu Phe Gly Thr Thr Glu Lys Leu 
                85                  90                  95      
Leu Gly Phe Ser Glu Arg Gly Ile Ala Leu Phe Ala Pro Gln Phe Asp 
            100                 105                 110         
Lys Leu Leu Asn Lys Asn Gln Lys Leu Ser Lys Ser Leu Gly Gly Ser 
        115                 120                 125             
Ser Glu Ala Leu Gly Gln Arg Leu Asn Lys Thr Gln Thr Ala Leu Ser 
    130                 135                 140                 
Ala Leu Gln Ser Phe Leu Gly Thr Ala Ile Ala Gly Met Asp Leu Asp 
145                 150                 155                 160 
Ser Leu Leu Arg Arg Arg Arg Asn Gly Glu Asp Val Ser Gly Ser Glu 
                165                 170                 175     
Leu Ala Lys Ala Gly Val Asp Leu Ala Ala Gln Leu Val Asp Asn Ile 
            180                 185                 190         
Ala Ser Ala Thr Gly Thr Val Asp Ala Phe Ala Glu Gln Leu Gly Lys 
        195                 200                 205             
Leu Gly Asn Ala Leu Ser Asn Thr Arg Leu Ser Gly Leu Ala Ser Lys 
    210                 215                 220                 
Leu Asn Asn Leu Pro Asp Leu Ser Leu Ala Gly Pro Gly Phe Asp Ala 
225                 230                 235                 240 
Val Ser Gly Ile Leu Ser Val Val Ser Ala Ser Phe Ile Leu Ser Asn 
                245                 250                 255     
Lys Asp Ala Asp Ala Gly Thr Lys Ala Ala Ala Gly Ile Glu Ile Ser 
            260                 265                 270         
Thr Lys Ile Leu Gly Asn Ile Gly Lys Ala Val Ser Gln Tyr Ile Ile 
        275                 280                 285             
Ala Gln Arg Val Ala Ala Gly Leu Ser Thr Thr Ala Ala Thr Gly Gly 
    290                 295                 300                 
Leu Ile Gly Ser Val Val Ala Leu Ala Ile Ser Pro Leu Ser Phe Leu 
305                 310                 315                 320 
Asn Val Ala Asp Lys Phe Glu Arg Ala Lys Gln Leu Glu Gln Tyr Ser 
                325                 330                 335     
Glu Arg Phe Lys Lys Phe Gly Tyr Glu Gly Asp Ser Leu Leu Ala Ser 
            340                 345                 350         
Phe Tyr Arg Glu Thr Gly Ala Ile Glu Ala Ala Leu Thr Thr Ile Asn 
        355                 360                 365             
Ser Val Leu Ser Ala Ala Ser Ala Gly Val Gly Ala Ala Ala Thr Gly 
    370                 375                 380                 
Ser Leu Val Gly Ala Pro Val Ala Ala Leu Val Ser Ala Ile Thr Gly 
385                 390                 395                 400 
Ile Ile Ser Gly Ile Leu Asp Ala Ser Lys Gln Ala Ile Phe Glu Arg 
                405                 410                 415     
Val Ala Thr Lys Leu Ala Asn Lys Ile Asp Glu Trp Glu Lys Lys His 
            420                 425                 430         
Gly Lys Asn Tyr Phe Glu Asn Gly Tyr Asp Ala Arg His Ser Ala Phe 
        435                 440                 445             
Leu Glu Asp Thr Phe Glu Leu Leu Ser Gln Tyr Asn Lys Glu Tyr Ser 
    450                 455                 460                 
Val Glu Arg Val Val Ala Ile Thr Gln Gln Arg Trp Asp Val Asn Ile 
465                 470                 475                 480 
Gly Glu Leu Ala Gly Ile Thr Arg Lys Gly Ala Asp Ala Lys Ser Gly 
                485                 490                 495     
Lys Ala Tyr Val Asp Phe Phe Glu Glu Gly Lys Leu Leu Glu Lys Asp 
            500                 505                 510         
Pro Asp Arg Phe Asp Lys Lys Val Phe Asp Pro Leu Glu Gly Lys Ile 
        515                 520                 525             
Asp Leu Ser Ser Ile Asn Lys Thr Thr Leu Leu Lys Phe Ile Thr Pro 
    530                 535                 540                 
Val Phe Thr Ala Gly Glu Glu Ile Arg Glu Arg Lys Gln Thr Gly Ala 
545                 550                 555                 560 
Tyr Glu Tyr Met Thr Glu Leu Phe Val Lys Gly Lys Glu Lys Trp Val 
                565                 570                 575     
Val Thr Gly Val Gln Ser His Asn Ala Ile Tyr Asp Tyr Thr Asn Leu 
            580                 585                 590         
Ile Gln Leu Ala Ile Asp Lys Lys Gly Glu Lys Arg Gln Val Thr Ile 
        595                 600                 605             
Glu Ser His Leu Gly Glu Lys Asn Asp Arg Ile Tyr Leu Ser Ser Gly 
    610                 615                 620                 
Ser Ser Ile Val Tyr Ala Gly Asn Gly His Asp Val Ala Tyr Tyr Asp 
625                 630                 635                 640 
Lys Thr Asp Thr Gly Tyr Leu Thr Phe Asp Gly Gln Ser Ala Gln Lys 
                645                 650                 655     
Ala Gly Glu Tyr Ile Val Thr Lys Glu Leu Lys Ala Asp Val Lys Val 
            660                 665                 670         
Leu Lys Glu Val Val Lys Thr Gln Asp Ile Ser Val Gly Ala Arg Ser 
        675                 680                 685             
Glu Lys Leu Glu Tyr Arg Asp Tyr Glu Leu Ser Pro Phe Glu Leu Gly 
    690                 695                 700                 
Asn Gly Ile Arg Ala Lys Asp Glu Leu His Ser Val Glu Glu Ile Ile 
705                 710                 715                 720 
Gly Ser Asn Arg Lys Asp Lys Phe Phe Gly Ser Arg Phe Thr Asp Ile 
                725                 730                 735     
Phe His Gly Ala Lys Gly Asp Asp Glu Ile Tyr Gly Asn Asp Gly His 
            740                 745                 750         
Asp Ile Leu Tyr Gly Asp Asp Gly Asn Asp Val Ile His Gly Gly Asp 
        755                 760                 765             
Gly Asn Asp His Leu Val Gly Gly Asn Gly Asn Asp Arg Leu Ile Gly 
    770                 775                 780                 
Gly Lys Gly Asn Asn Phe Leu Asn Gly Gly Asp Gly Asp Asp Glu Leu 
785                 790                 795                 800 
Gln Val Phe Glu Gly Gln Tyr Asn Val Leu Leu Gly Gly Ala Gly Asn 
                805                 810                 815     
Asp Ile Leu Tyr Gly Ser Asp Gly Thr Asn Leu Phe Asp Gly Gly Val 
            820                 825                 830         
Gly Asn Asp Lys Ile Tyr Gly Gly Leu Gly Lys Asp Ile Tyr Arg Tyr 
        835                 840                 845             
Ser Lys Glu Tyr Gly Arg His Ile Ile Ile Glu Lys Gly Gly Asp Asp 
    850                 855                 860                 
Asp Thr Leu Leu Leu Ser Asp Leu Ser Phe Lys Asp Val Gly Phe Ile 
865                 870                 875                 880 
Arg Ile Gly Asp Asp Leu Leu Val Asn Lys Arg Ile Gly Gly Thr Leu 
                885                 890                 895     
Tyr Tyr His Glu Asp Tyr Asn Gly Asn Ala Leu Thr Ile Lys Asp Trp 
            900                 905                 910         
Phe Lys Glu Gly Lys Glu Gly Gln Asn Asn Lys Ile Glu Lys Ile Val 
        915                 920                 925             
Asp Lys Asp Gly Ala Tyr Val Leu Ser Gln Tyr Leu Thr Glu Leu Thr 
    930                 935                 940                 
Ala Pro Gly Arg Gly Ile Asn Tyr Phe Asn Gly Leu Glu Glu Lys Leu 
945                 950                 955                 960 
Tyr Tyr Gly Glu Gly Tyr Asn Ala Leu Pro Gln Leu Arg Lys Asp Ile 
                965                 970                 975     
Glu Gln Ile Ile Ser Ser Thr Gly Ala Leu Thr Gly Glu His Gly Gln 
            980                 985                 990         
Val Leu Val Gly Ala Gly Gly Pro Leu Ala Tyr Ser Asn Ser Pro Asn 
        995                 1000                1005            
Ser Ile Pro Asn Ala Phe Ser Asn Tyr Leu Thr Gln Ser Ala 
    1010                1015                1020        

<210> 5
<211> 956
<212> PRT
<213> Actonbacillus pleuropneumoniae


<220> 
<223> ApxIIA S148G K557A N687A

<400> 5
Met Ser Lys Ile Thr Leu Ser Ser Leu Lys Ser Ser Leu Gln Gln Gly 
1               5                   10                  15      
Leu Lys Asn Gly Lys Asn Lys Leu Asn Gln Ala Gly Thr Thr Leu Lys 
            20                  25                  30          
Asn Gly Leu Thr Gln Thr Gly His Ser Leu Gln Asn Gly Ala Lys Lys 
        35                  40                  45              
Leu Ile Leu Tyr Ile Pro Gln Gly Tyr Asp Ser Gly Gln Gly Asn Gly 
    50                  55                  60                  
Val Gln Asp Leu Val Lys Ala Ala Asn Asp Leu Gly Ile Glu Val Trp 
65                  70                  75                  80  
Arg Glu Glu Arg Ser Asn Leu Asp Ile Ala Lys Thr Ser Phe Asp Thr 
                85                  90                  95      
Thr Gln Lys Ile Leu Gly Phe Thr Asp Arg Gly Ile Val Leu Phe Ala 
            100                 105                 110         
Pro Gln Leu Asp Asn Leu Leu Lys Lys Asn Pro Lys Ile Gly Asn Thr 
        115                 120                 125             
Leu Gly Ser Ala Ser Ser Ile Ser Gln Asn Ile Gly Lys Ala Asn Thr 
    130                 135                 140                 
Val Leu Gly Gly Ile Gln Ser Ile Leu Gly Ser Val Leu Ser Gly Val 
145                 150                 155                 160 
Asn Leu Asn Glu Leu Leu Gln Asn Lys Asp Pro Asn Gln Leu Glu Leu 
                165                 170                 175     
Ala Lys Ala Gly Leu Glu Leu Thr Asn Glu Leu Val Gly Asn Ile Ala 
            180                 185                 190         
Ser Ser Val Gln Thr Val Asp Ala Phe Ala Glu Gln Ile Ser Lys Leu 
        195                 200                 205             
Gly Ser His Leu Gln Asn Val Lys Gly Leu Gly Gly Leu Ser Asn Lys 
    210                 215                 220                 
Leu Gln Asn Leu Pro Asp Leu Gly Lys Ala Ser Leu Gly Leu Asp Ile 
225                 230                 235                 240 
Ile Ser Gly Leu Leu Ser Gly Ala Ser Ala Gly Leu Ile Leu Ala Asp 
                245                 250                 255     
Lys Glu Ala Ser Thr Glu Lys Lys Ala Ala Ala Gly Val Glu Phe Ala 
            260                 265                 270         
Asn Gln Ile Ile Gly Asn Val Thr Lys Ala Val Ser Ser Tyr Ile Leu 
        275                 280                 285             
Ala Gln Arg Val Ala Ser Gly Leu Ser Ser Thr Gly Pro Val Ala Ala 
    290                 295                 300                 
Leu Ile Ala Ser Thr Val Ala Leu Ala Val Ser Pro Leu Ser Phe Leu 
305                 310                 315                 320 
Asn Val Ala Asp Lys Phe Lys Gln Ala Asp Leu Ile Lys Ser Tyr Ser 
                325                 330                 335     
Glu Arg Phe Gln Lys Leu Gly Tyr Asp Gly Asp Arg Leu Leu Ala Asp 
            340                 345                 350         
Phe His Arg Glu Thr Gly Thr Ile Asp Ala Ser Val Thr Thr Ile Asn 
        355                 360                 365             
Thr Ala Leu Ala Ala Ile Ser Gly Gly Val Gly Ala Ala Ser Ala Gly 
    370                 375                 380                 
Ser Leu Val Gly Ala Pro Val Ala Leu Leu Val Ala Gly Val Thr Gly 
385                 390                 395                 400 
Leu Ile Thr Thr Ile Leu Glu Tyr Ser Lys Gln Ala Met Phe Glu His 
                405                 410                 415     
Val Ala Asn Lys Val His Asp Arg Ile Val Glu Trp Glu Lys Lys His 
            420                 425                 430         
Asn Lys Asn Tyr Phe Glu Gln Gly Tyr Asp Ser Arg His Leu Ala Asp 
        435                 440                 445             
Leu Gln Asp Asn Met Lys Phe Leu Ile Asn Leu Asn Lys Glu Leu Gln 
    450                 455                 460                 
Ala Glu Arg Val Val Ala Ile Thr Gln Gln Arg Trp Asp Asn Gln Ile 
465                 470                 475                 480 
Gly Asp Leu Ala Ala Ile Ser Arg Arg Thr Asp Lys Ile Ser Ser Gly 
                485                 490                 495     
Lys Ala Tyr Val Asp Ala Phe Glu Glu Gly Gln His Gln Ser Tyr Asp 
            500                 505                 510         
Ser Ser Val Gln Leu Asp Asn Lys Asn Gly Ile Ile Asn Ile Ser Asn 
        515                 520                 525             
Thr Asn Arg Lys Thr Gln Ser Val Leu Phe Arg Thr Pro Leu Leu Thr 
    530                 535                 540                 
Pro Gly Glu Glu Asn Arg Glu Arg Ile Gln Glu Gly Ala Asn Ser Tyr 
545                 550                 555                 560 
Ile Thr Lys Leu His Ile Gln Arg Val Asp Ser Trp Thr Val Thr Asp 
                565                 570                 575     
Gly Asp Ala Ser Ser Ser Val Asp Phe Thr Asn Val Val Gln Arg Ile 
            580                 585                 590         
Ala Val Lys Phe Asp Asp Ala Gly Asn Ile Ile Glu Ser Lys Asp Thr 
        595                 600                 605             
Lys Ile Ile Ala Asn Leu Gly Ala Gly Asn Asp Asn Val Phe Val Gly 
    610                 615                 620                 
Ser Ser Thr Thr Val Ile Asp Gly Gly Asp Gly His Asp Arg Val His 
625                 630                 635                 640 
Tyr Ser Arg Gly Glu Tyr Gly Ala Leu Val Ile Asp Ala Thr Ala Glu 
                645                 650                 655     
Thr Glu Lys Gly Ser Tyr Ser Val Lys Arg Tyr Val Gly Asp Ser Lys 
            660                 665                 670         
Ala Leu His Glu Thr Ile Ala Thr His Gln Thr Asn Val Gly Ala Arg 
        675                 680                 685             
Glu Glu Lys Ile Glu Tyr Arg Arg Glu Asp Asp Arg Phe His Thr Gly 
    690                 695                 700                 
Tyr Thr Val Thr Asp Ser Leu Lys Ser Val Glu Glu Ile Ile Gly Ser 
705                 710                 715                 720 
Gln Phe Asn Asp Ile Phe Lys Gly Ser Gln Phe Asp Asp Val Phe His 
                725                 730                 735     
Gly Gly Asn Gly Val Asp Thr Ile Asp Gly Asn Asp Gly Asp Asp His 
            740                 745                 750         
Leu Phe Gly Gly Ala Gly Asp Asp Val Ile Asp Gly Gly Asn Gly Asn 
        755                 760                 765             
Asn Phe Leu Val Gly Gly Thr Gly Asn Asp Ile Ile Ser Gly Gly Lys 
    770                 775                 780                 
Asp Asn Asp Ile Tyr Val His Lys Thr Gly Asp Gly Asn Asp Ser Ile 
785                 790                 795                 800 
Thr Asp Ser Gly Gly Gln Asp Lys Leu Ala Phe Ser Asp Val Asn Leu 
                805                 810                 815     
Lys Asp Leu Thr Phe Lys Lys Val Asp Ser Ser Leu Glu Ile Ile Asn 
            820                 825                 830         
Gln Lys Gly Glu Lys Val Arg Ile Gly Asn Trp Phe Leu Glu Asp Asp 
        835                 840                 845             
Leu Ala Ser Thr Val Ala Asn Tyr Lys Ala Thr Asn Asp Arg Lys Ile 
    850                 855                 860                 
Glu Glu Ile Ile Gly Lys Gly Gly Glu Arg Ile Thr Ser Glu Gln Val 
865                 870                 875                 880 
Asp Lys Leu Ile Lys Glu Gly Asn Asn Gln Ile Ser Ala Glu Ala Leu 
                885                 890                 895     
Ser Lys Val Val Asn Asp Tyr Asn Thr Ser Lys Asp Arg Gln Asn Val 
            900                 905                 910         
Ser Asn Ser Leu Ala Lys Leu Ile Ser Ser Val Gly Ser Phe Thr Ser 
        915                 920                 925             
Ser Ser Asp Phe Arg Asn Asn Leu Gly Thr Tyr Val Pro Ser Ser Ile 
    930                 935                 940                 
Asp Val Ser Asn Asn Ile Gln Leu Ala Arg Ala Ala 
945                 950                 955     

<210> 6
<211> 1052
<212> PRT
<213> Actonbacillus pleuropneumoniae


<220> 
<223> ApxIIIA K571A K702A

<400> 6
Met Ser Thr Trp Ser Ser Met Leu Ala Asp Leu Lys Lys Arg Ala Glu 
1               5                   10                  15      
Glu Ala Lys Arg Gln Ala Lys Lys Gly Tyr Asp Val Thr Lys Asn Gly 
            20                  25                  30          
Leu Gln Tyr Gly Val Ser Gln Ala Lys Leu Gln Ala Leu Ala Ala Gly 
        35                  40                  45              
Lys Ala Val Gln Lys Tyr Gly Asn Lys Leu Val Leu Val Ile Pro Lys 
    50                  55                  60                  
Glu Tyr Asp Gly Ser Val Gly Asn Gly Phe Phe Asp Leu Val Lys Ala 
65                  70                  75                  80  
Ala Glu Glu Leu Gly Ile Gln Val Lys Tyr Val Asn Arg Asn Glu Leu 
                85                  90                  95      
Glu Val Ala His Lys Ser Leu Gly Thr Ala Asp Gln Phe Leu Gly Leu 
            100                 105                 110         
Thr Glu Arg Gly Leu Thr Leu Phe Ala Pro Gln Leu Asp Gln Phe Leu 
        115                 120                 125             
Gln Lys His Ser Lys Ile Ser Asn Val Val Gly Ser Ser Thr Gly Asp 
    130                 135                 140                 
Ala Val Ser Lys Leu Ala Lys Ser Gln Thr Ile Ile Ser Gly Ile Gln 
145                 150                 155                 160 
Ser Val Leu Gly Thr Val Leu Ala Gly Ile Asn Leu Asn Glu Ala Ile 
                165                 170                 175     
Ile Ser Gly Gly Ser Glu Leu Glu Leu Ala Glu Ala Gly Val Ser Leu 
            180                 185                 190         
Ala Ser Glu Leu Val Ser Asn Ile Ala Lys Gly Thr Thr Thr Ile Asp 
        195                 200                 205             
Ala Phe Thr Thr Gln Ile Gln Asn Phe Gly Lys Leu Val Glu Asn Ala 
    210                 215                 220                 
Lys Gly Leu Gly Gly Val Gly Arg Gln Leu Gln Asn Ile Ser Gly Ser 
225                 230                 235                 240 
Ala Leu Ser Lys Thr Gly Leu Gly Leu Asp Ile Ile Ser Ser Leu Leu 
                245                 250                 255     
Ser Gly Val Thr Ala Ser Phe Ala Leu Ala Asn Lys Asn Ala Ser Thr 
            260                 265                 270         
Ser Thr Lys Val Ala Ala Gly Phe Glu Leu Ser Asn Gln Val Ile Gly 
        275                 280                 285             
Gly Ile Thr Lys Ala Val Ser Ser Tyr Ile Leu Ala Gln Arg Leu Ala 
    290                 295                 300                 
Ala Gly Leu Ser Thr Thr Gly Pro Ala Ala Ala Leu Ile Ala Ser Ser 
305                 310                 315                 320 
Ile Ser Leu Ala Ile Ser Pro Leu Ala Phe Leu Arg Val Ala Asp Asn 
                325                 330                 335     
Phe Asn Arg Ser Lys Glu Ile Gly Glu Phe Ala Glu Arg Phe Lys Lys 
            340                 345                 350         
Leu Gly Tyr Asp Gly Asp Lys Leu Leu Ser Glu Phe Tyr His Glu Ala 
        355                 360                 365             
Gly Thr Ile Asp Ala Ser Ile Thr Thr Ile Ser Thr Ala Leu Ser Ala 
    370                 375                 380                 
Ile Ala Ala Gly Thr Ala Ala Ala Ser Ala Gly Ala Leu Val Gly Ala 
385                 390                 395                 400 
Pro Ile Thr Leu Leu Val Thr Gly Ile Thr Gly Leu Ile Ser Gly Ile 
                405                 410                 415     
Leu Glu Phe Ser Lys Gln Pro Met Leu Asp His Val Ala Ser Lys Ile 
            420                 425                 430         
Gly Asn Lys Ile Asp Glu Trp Glu Lys Lys Tyr Gly Lys Asn Tyr Phe 
        435                 440                 445             
Glu Asn Gly Tyr Asp Ala Arg His Lys Ala Phe Leu Glu Asp Ser Phe 
    450                 455                 460                 
Ser Leu Leu Ser Ser Phe Asn Lys Gln Tyr Glu Thr Glu Arg Ala Val 
465                 470                 475                 480 
Leu Ile Thr Gln Gln Arg Trp Asp Glu Tyr Ile Gly Glu Leu Ala Gly 
                485                 490                 495     
Ile Thr Gly Lys Gly Asp Lys Leu Ser Ser Gly Lys Ala Tyr Val Asp 
            500                 505                 510         
Tyr Phe Gln Glu Gly Lys Leu Leu Glu Lys Lys Pro Asp Asp Phe Ser 
        515                 520                 525             
Lys Val Val Phe Asp Pro Thr Lys Gly Glu Ile Asp Ile Ser Asn Ser 
    530                 535                 540                 
Gln Thr Ser Thr Leu Leu Lys Phe Val Thr Pro Leu Leu Thr Pro Gly 
545                 550                 555                 560 
Thr Glu Ser Arg Glu Arg Thr Gln Thr Gly Ala Tyr Glu Tyr Ile Thr 
                565                 570                 575     
Lys Leu Val Val Lys Gly Lys Asp Lys Trp Val Val Asn Gly Val Lys 
            580                 585                 590         
Asp Lys Gly Ala Val Tyr Asp Tyr Thr Asn Leu Ile Gln His Ala His 
        595                 600                 605             
Ile Ser Ser Ser Val Ala Arg Gly Glu Glu Tyr Arg Glu Val Arg Leu 
    610                 615                 620                 
Val Ser His Leu Gly Asn Gly Asn Asp Lys Val Phe Leu Ala Ala Gly 
625                 630                 635                 640 
Ser Ala Glu Ile His Ala Gly Glu Gly His Asp Val Val Tyr Tyr Asp 
                645                 650                 655     
Lys Thr Asp Thr Gly Leu Leu Val Ile Asp Gly Thr Lys Ala Thr Glu 
            660                 665                 670         
Gln Gly Arg Tyr Ser Val Thr Arg Glu Leu Ser Gly Ala Thr Lys Ile 
        675                 680                 685             
Leu Arg Glu Val Ile Lys Asn Gln Lys Ser Ala Val Gly Ala Arg Glu 
    690                 695                 700                 
Glu Thr Leu Glu Tyr Arg Asp Tyr Glu Leu Thr Gln Ser Gly Asn Ser 
705                 710                 715                 720 
Asn Leu Lys Ala His Asp Glu Leu His Ser Val Glu Glu Ile Ile Gly 
                725                 730                 735     
Ser Asn Gln Arg Asp Glu Phe Lys Gly Ser Lys Phe Arg Asp Ile Phe 
            740                 745                 750         
His Gly Ala Asp Gly Asp Asp Leu Leu Asn Gly Asn Asp Gly Asp Asp 
        755                 760                 765             
Ile Leu Tyr Gly Asp Lys Gly Asn Asp Glu Leu Arg Gly Asp Asn Gly 
    770                 775                 780                 
Asn Asp Gln Leu Tyr Gly Gly Glu Gly Asn Asp Lys Leu Leu Gly Gly 
785                 790                 795                 800 
Asn Gly Asn Asn Tyr Leu Ser Gly Gly Asp Gly Asn Asp Glu Leu Gln 
                805                 810                 815     
Val Leu Gly Asn Gly Phe Asn Val Leu Arg Gly Gly Lys Gly Asp Asp 
            820                 825                 830         
Lys Leu Tyr Gly Ser Ser Gly Ser Asp Leu Leu Asp Gly Gly Glu Gly 
        835                 840                 845             
Asn Asp Tyr Leu Glu Gly Gly Asp Gly Ser Asp Phe Tyr Val Tyr Arg 
    850                 855                 860                 
Ser Thr Ser Gly Asn His Thr Ile Tyr Asp Gln Gly Lys Ser Ser Asp 
865                 870                 875                 880 
Leu Asp Lys Leu Tyr Leu Ser Asp Phe Ser Phe Asp Arg Leu Leu Val 
                885                 890                 895     
Glu Lys Val Asp Asp Asn Leu Val Leu Arg Ser Asn Glu Ser Ser His 
            900                 905                 910         
Asn Asn Gly Val Leu Thr Ile Lys Asp Trp Phe Lys Glu Gly Asn Lys 
        915                 920                 925             
Tyr Asn His Lys Ile Glu Gln Ile Val Asp Lys Asn Gly Arg Lys Leu 
    930                 935                 940                 
Thr Ala Glu Asn Leu Gly Thr Tyr Phe Lys Asn Ala Pro Lys Ala Asp 
945                 950                 955                 960 
Asn Leu Leu Asn Tyr Ala Thr Lys Glu Asp Gln Asn Glu Ser Asn Leu 
                965                 970                 975     
Ser Ser Leu Lys Thr Glu Leu Ser Lys Ile Ile Thr Asn Ala Gly Asn 
            980                 985                 990         
Phe Gly Val Ala Lys Gln Gly Asn Thr Gly Ile Asn Thr Ala Ala Leu 
        995                 1000                1005            
Asn Asn Glu Val Asn Lys Ile Ile Ser Ser Ala Asn Thr Phe Ala Thr 
    1010                1015                1020                
Ser Gln Leu Gly Gly Ser Gly Met Gly Thr Leu Pro Ser Thr Asn Val 
1025                1030                1035                1040
Asn Ser Met Met Leu Gly Asn Leu Ala Arg Ala Ala 
                1045                1050        

<210> 7
<211> 3078
<212> DNA
<213> Actonbacillus pleuropneumoniae


<220> 
<223> ApxIA wild type

<400> 7
ggatccgcaa attctcaact ggaccgcgta aagggcctca ttgacagcct caaccaacac      60

accaaatcgg ctgcgaaatc cggtgcgggt gcgctgaaaa atggtctggg tcaggtgaaa     120

caggcgggtc agaaactgat cttatacatc ccgaaagatt atcaggcttc aaccggctcc     180

agtcttaatg atttggtaaa agccgccgaa gcactgggta ttgaagtaca tcgcagcgaa     240

aaaaatggga ctgcgttagc gaaagagctg tttggcacaa cggaaaagct gctgggcttt     300

agtgaacgtg gcattgcgct gtttgcacca cagttcgata aattgttaaa taagaatcag     360

aaactgagca agtccttagg cggctcatca gaagccctgg gtcagcgtct gaacaaaact     420

cagacagccc tgagtgcatt acagtcattt ctcgggacgg caattgcagg catggatctg     480

gatagcctgc ttcgccgtcg gcgtaacggt gaggatgtct cgggcagcga gctggcaaaa     540

gccggcgttg acctggcagc ccagttggtt gacaacatcg catctgccac cggcactgtt     600

gacgcttttg ccgaacaact gggcaaatta gggaacgcgc tgtctaacac gcgcttatct     660

gggctcgcat ccaaactcaa caacttgccg gacttgtcgc tggcaggacc tggctttgac     720

gcggtttcag gcattctgtc cgtggttagt gcttcgttca ttcttagcaa caaagacgcc     780

gatgcgggta ccaaggcggc cgctgggatt gaaatcagca cgaaaatctt gggtaatatt     840

ggcaaggcag tgtcgcagta tatcatcgct caacgtgtgg ccgcaggcct cagcaccacc     900

gcggccacag gtggcctgat tggtagtgtc gtcgctctgg ccatttctcc cctgtcgttc     960

ctgaacgttg cggacaaatt tgaacgcgct aaacagctgg aacagtattc tgaacgcttt    1020

aaaaaatttg gatatgaagg cgatagcctg ctggcatcct tttaccgcga aaccggtgcg    1080

attgaagccg cattgaccac cattaactcg gtactcagtg ctgccagtgc aggggtggga    1140

gcggcagcca caggttctct ggtcggagcc ccggttgctg ccttagtgtc tgccattacc    1200

gggattatct cgggcatcct tgatgcgtct aaacaggcga ttttcgaacg cgtggcgacg    1260

aaactggcga acaaaattga tgaatgggaa aagaaacacg gtaagaacta ctttgagaac    1320

ggctatgatg cccgtcatag tgcgtttctc gaagatacct ttgaactttt gtcgcaatac    1380

aacaaagagt atagcgtgga gcgcgtagtt gctattacac agcagcgttg ggatgtgaat    1440

attggggaat tggcggggat cacccgtaaa ggcgcggatg caaaatccgg taaagcttac    1500

gtcgatttct tcgaggaagg gaaactgctg gaaaaggacc cggatcgctt tgataagaaa    1560

gtgttcgacc cgcttgaggg taaaattgac ttgtcatcca ttaacaaaac gacgttgctg    1620

aaattcatta cgcccgtgtt tacggcggga gaagaaattc gcgaacgcaa acagactggg    1680

aaatacgagt acatgactga actgtttgtt aaagggaagg aaaagtgggt agtcaccggt    1740

gttcagtcgc acaatgcgat ttacgattat actaacctga ttcagctggc aatcgacaag    1800

aaaggtgaaa aacgccaagt gaccatcgag agtcaccttg gcgaaaagaa cgatcgtatc    1860

tatctgagca gtggcagctc aatcgtctat gccggcaacg gtcatgacgt tgcctattac    1920

gataaaacgg ataccggata tctgacgttt gacggtcaaa gcgcgcagaa agcgggtgaa    1980

tatatcgtca ctaaggaatt gaaagccgac gttaaagtcc tgaaagaggt ggtcaaaacc    2040

caagatatct cagtaggcaa acgcagcgag aaactggagt accgcgatta tgagctgagc    2100

ccgtttgaac ttggtaacgg gattcgtgcg aaagatgaac tgcatagcgt ggaagagatt    2160

attggatcga atcggaaaga caaattcttt ggtagccgct tcaccgatat ctttcatggc    2220

gccaaaggcg acgacgagat ttatgggaat gatgggcatg atatcctgta tggcgacgat    2280

ggcaacgatg tgattcatgg aggcgacggt aatgatcact tagtgggcgg caatgggaat    2340

gaccgtctga tcggcgggaa aggcaataat ttcctgaatg gcggtgatgg cgatgatgaa    2400

cttcaggtgt tcgaggggca atataatgtg ctgttaggcg gcgccggcaa cgatatttta    2460

tacggtagtg atggtaccaa cctcttcgat ggtggcgtgg gtaatgataa aatctatggt    2520

ggcctgggca aagatatcta tcgctattct aaagaatacg gtcggcatat tatcattgaa    2580

aaaggtggtg acgacgacac cctgctgttg agcgatcttt ccttcaagga cgtcggtttc    2640

attcgtattg gagatgatct gctggtgaac aaacggatcg gcggcaccct gtattaccac    2700

gaggattaca acggtaatgc gcttacaatc aaagattggt ttaaagaggg taaggaaggt    2760

cagaataaca aaattgaaaa gatcgttgac aaagatggag cttatgtcct gtcccagtat    2820

ttgaccgaac tcacggcgcc tggtcgtggc attaattact tcaacggatt agaagagaag    2880

ttatactacg gtgaaggcta caacgcgctc ccgcaactgc gcaaagatat tgaacaaatc    2940

atctccagca ccggagcttt aacgggcgaa catggccaag ttctggtagg agcgggtggc    3000

ccactggcgt attcaaattc tccgaacagc attccaaacg cgttttcgaa ttatctgact    3060

cagagcgctt aagagctc                                                  3078


<210> 8
<211> 2880
<212> DNA
<213> Actonbacillus pleuropneumoniae


<220> 
<223> ApxIIA wild type

<400> 8
ggatcctcga aaattacctt gtccagcctg aaatccagct tacagcaagg tctgaagaat      60

gggaagaaca aactgaacca agccggtacc acgctgaaga atggattgac gcaaacaggt     120

catagtctgc agaatggtgc gaagaaatta atcctctaca ttccgcaggg ctatgactca     180

ggccaaggca acggggtcca ggacctcgta aaagccgcga atgatctggg cattgaggtg     240

tggcgcgaag aacgtagtaa cctggatatt gcgaaaactt cgttcgatac cacccagaaa     300

attctgggat tcaccgaccg gggcatcgtg ttgttcgcac cccagctgga taatctcctg     360

aagaagaacc cgaaaatcgg caacacgctg ggcagcgcat caagcatctc gcagaacatc     420

ggcaaagcca ataccgtctt aggtggaatc cagagcattc tgggtagcgt gctttctggc     480

gtgaacttaa acgaattact tcagaacaaa gacccgaatc agctggaact ggcgaaagct     540

ggactggaac ttacgaacga actggtaggt aacattgcca gctctgtaca aaccgttgat     600

gcgtttgcgg agcagatcag taaactgggt tctcatttgc aaaatgttaa agggctgggt     660

ggtctgagta acaaattgca gaatctgccg gacctgggca aggcttcttt ggggctggat     720

attatctcgg ggctgttgtc tggcgcaagc gctggcctga ttttagcgga taaggaggct     780

tcgaccgaaa agaaggccgc tgccggcgta gaatttgcga accagattat tgggaacgtg     840

accaaagcgg tgagtagcta tattttggcg caacgtgttg cctccggttt gagtagtact     900

ggtccagtcg cagcactgat tgccagtact gtggcactgg cggttagtcc actgtccttt     960

ctgaatgtcg cggataaatt taaacaagcc gatctcatca aatcttatag tgaacgtttc    1020

cagaaattag gttatgatgg cgatcgctta ctggcggact ttcaccggga aacaggcacc    1080

attgatgcaa gcgtgaccac gattaatacc gcgcttgcag caatcagcgg tggtgttggg    1140

gcagcgagtg ctggaagcct ggttggcgcg cctgttgctc tgcttgtagc gggtgtaacg    1200

ggcttaatca caacgatcct cgaatattcc aaacaagcga tgtttgagca tgtcgctaac    1260

aaagtccacg accgcatcgt ggaatgggaa aaaaaacaca ataaaaacta ctttgagcag    1320

ggctacgaca gccgtcattt ggcagatctg caggacaata tgaaattcct catcaactta    1380

aacaaagaac ttcaggctga acgtgtcgtg gcgattacgc aacaacgttg ggataaccag    1440

attggcgacc tggcggccat ttcccgtcgc actgacaaaa tttcctcggg taaagcatat    1500

gtggacgcgt ttgaggaagg tcagcaccag agctatgata gttcagtgca gttagacaat    1560

aaaaatggta ttattaacat ttcaaacaca aaccgcaaga cccaatcagt actgtttcgc    1620

acgccgttac tgaccccggg tgaagaaaac cgcgagcgca ttcaggaggg taagaactcc    1680

tacattacca aacttcacat tcagcgcgta gattcatgga cggtgacgga tggcgatgcg    1740

tcatcgagcg tagatttcac caatgtggtt cagcgtattg cggtgaaatt cgatgatgca    1800

ggaaacatta tcgagtcaaa agatactaaa attatcgcca atctcggtgc cggtaatgac    1860

aatgtctttg taggcagcag cacgacggtg attgacggcg gtgacggcca tgatcgcgtt    1920

cattattcgc gtggcgaata cggcgctctg gtcattgacg caactgccga gacagagaaa    1980

ggtagctact ccgttaaacg ttatgttggt gactcaaaag cgctgcacga aacgattgcc    2040

actcatcaga ccaatgtggg gaatcgcgaa gagaaaatcg agtatcgccg cgaagatgat    2100

cggttccata ccgggtatac tgtgaccgat tcgctgaaaa gcgtcgaaga aattatcgga    2160

tctcaattca atgacatctt caaaggttcc cagtttgatg acgtgtttca tggcggtaat    2220

ggcgttgata ccattgacgg caacgatggc gacgaccatc tgtttggcgg ggctggtgat    2280

gacgtgatcg atggcggcaa cggcaacaac tttctggtgg gtggtaccgg gaacgatatc    2340

atttcaggcg gcaaagataa cgacatctac gttcacaaga caggggatgg gaatgatagc    2400

attactgatt cgggtggcca agataagctg gccttttctg acgttaatct gaaagatctg    2460

actttcaaaa aggtggattc tagcctggaa atcattaatc agaaaggaga gaaggtccgc    2520

attggaaatt ggtttctcga agatgatctt gcctcgaccg tggcaaacta caaagcgacc    2580

aatgaccgca aaatcgaaga aatcattggg aaaggcggtg aacgcatcac ctctgaacag    2640

gtggataaac tgatcaaaga aggcaataac cagatttccg ctgaagcgtt gtctaaagtc    2700

gtcaacgatt acaacacgtc caaagatcgc caaaacgtta gcaattcact tgccaaactg    2760

atctcgagcg ttggatcttt tacatcctcg agcgatttcc gtaacaacct cggcacctat    2820

gttccttctt cgattgatgt cagtaataat atccaactgg cccgtgccgc ctaagagctc    2880


<210> 9
<211> 3168
<212> DNA
<213> Actonbacillus pleuropneumoniae


<220> 
<223> ApxIIIA wild type

<400> 9
ggatcctcta cctggtcctc catgctggct gatctgaaga agcgtgcgga agaagccaaa       60

cgtcaggcga aaaaaggtta tgacgtcacg aagaacggcc tgcagtacgg tgttagtcaa      120

gccaaactgc aggctctggc agccggtaaa gctgttcaga aatacggtaa caaacttgtg      180

ttagtaattc cgaaagaata tgatgggtcg gtgggtaatg gcttcttcga cctggtcaaa      240

gccgcggaag aactgggcat tcaggtgaaa tatgtcaacc gcaacgaact cgaggttgcg      300

cataaatctc tgggaacggc ggatcagttt ctgggtctga ctgagcgtgg cctgaccctg      360

tttgcaccgc agcttgacca gttcttacag aaacacagca aaatctcaaa tgtggttggt      420

tcttcaactg gtgacgcggt gagcaaactg gcaaagtctc agacaatcat ttctggaatc      480

cagtcggtgt tgggcactgt actcgctggg atcaacttaa atgaagccat catttcaggc      540

ggctctgaat tggagctggc ggaagctggc gtaagcctgg cgtcggaact ggtgtcgaat      600

atcgccaaag gcaccacgac aattgatgcg tttaccaccc aaattcagaa ttttggaaaa      660

ctggtcgaaa acgcgaaggg cctgggtggc gtgggtcgtc agctgcaaaa catttccggt      720

agcgccttga gcaaaactgg tttgggctta gacatcatca gcagcttgct gagtggagtc      780

acagcgagct ttgcgctggc aaacaagaat gcctctacct ccaccaaagt tgctgccggt      840

tttgaactgt cgaatcaagt tattggaggc attaccaaag cggtcagctc atatatcctg      900

gcccaacgtc tggcggcggg tttgagcacg actggacccg ctgcagcgct gattgcatct      960

agtattagtc tggccatcag cccgctggcg tttctgcgcg tagctgataa tttcaaccgc     1020

agcaaagaaa tcggtgagtt cgcggaacgc tttaagaaat taggctatga tggggataag     1080

ctgctgtctg aattttacca tgaggctggc accattgacg catctatcac gacaatttcg     1140

acagccctgt cagcgattgc cgcagggact gcagccgctt cagccggcgc cctggttggt     1200

gcaccgatta cgcttcttgt cacgggtatt accggcttga ttagcggcat cctggaattt     1260

tccaaacagc cgatgctgga ccatgtagca agtaaaattg gcaacaaaat cgacgagtgg     1320

gaaaagaaat atggtaagaa ctacttcgag aatggttatg atgctcgcca taaagccttc     1380

ttggaagatt cgttcagcct gctcagttcc ttcaataagc aatatgaaac cgaacgcgcc     1440

gtgctgatca cccaacagcg ctgggatgag tacattgggg aactggcggg tattacgggt     1500

aagggtgaca aattatcaag cgggaaggca tacgttgatt attttcagga gggtaaactt     1560

ctggagaaga aacccgacga tttcagcaaa gtagtgtttg atccgacaaa aggcgagatc     1620

gatatcagca attcccagac ctctaccctg ctgaaatttg tgacccctct cctgacccca     1680

ggaactgaaa gccgtgaacg cactcaaacg ggaaagtacg aatacattac taaactcgtg     1740

gtaaaaggga aagataaatg ggtcgtgaac ggcgtgaaag ataaaggcgc ggtatacgat     1800

tacaccaact taattcagca tgctcacatt tcctcttccg tggcgcgtgg cgaagagtat     1860

cgggaagtcc gcctggttag ccatttgggt aatggcaatg ataaagtctt tctggctgcg     1920

ggctcggcag aaattcacgc cggcgaaggc catgatgtcg tatattacga taaaacggac     1980

accggactcc tggtgatcga tggcacaaaa gcaaccgagc aagggcgcta tagtgttacg     2040

cgtgagctga gtggcgcgac caaaattttg cgtgaagtga tcaaaaatca gaaatcggca     2100

gttggcaagc gcgaagaaac gttagagtat cgcgattacg aactcacgca aagcggtaac     2160

agtaatctga aagcacacga cgaactgcat tctgttgaag aaattattgg cagcaatcag     2220

cgcgatgaat tcaaaggctc gaagtttcgt gatatctttc atggtgcaga cggggatgac     2280

ctcctgaacg gcaatgatgg ggatgacatt ttatatggag acaaagggaa cgatgaactg     2340

cgcggcgata acgggaacga ccaactgtac ggtggtgagg ggaatgataa actgttgggc     2400

gggaacggca ataactattt atcgggcgga gatggtaacg atgaacttca ggttctgggg     2460

aacggtttca acgtccttcg tggaggcaaa ggtgacgata aactttatgg tagcagcggc     2520

tccgacctcc ttgatggtgg agaaggcaac gattacttag aaggtggtga tgggagtgat     2580

ttctatgtgt atcggagcac cagtggcaat cacaccattt atgatcaggg taaatcctcc     2640

gatctggaca aattatacct gtcagatttc tcgtttgacc gcctcttggt ggagaaagtg     2700

gatgacaacc tcgttttgcg ttccaatgag agtagccaca ataatggcgt tttaaccatc     2760

aaagactggt ttaaagaagg gaacaagtac aaccacaaaa tcgagcagat tgtcgataag     2820

aatggccgca aactgacggc cgaaaatctg ggtacctatt ttaaaaacgc cccaaaagcg     2880

gacaaccttc tgaactatgc gaccaaagag gaccagaacg aatcaaattt atcttcattg     2940

aaaacggaac tgagtaaaat tatcactaat gcgggtaatt ttggcgttgc caaacaaggt     3000

aacaccggca ttaataccgc agcgcttaac aacgaagtga acaaaatcat ttcgtcagcg     3060

aacaccttcg ctacgagtca actcggtggc tcggggatgg gcacactgcc ttccacgaac     3120

gtgaatagca tgatgctggg caatcttgcc cgggcggcgt aagagctc                  3168


<210> 10
<211> 3078
<212> DNA
<213> Actonbacillus pleuropneumoniae


<220> 
<223> ApxIA K560A K686A

<400> 10
ggatccgcta attctcagtt agatcgtgtg aaaggattaa tcgatagcct gaaccagcac      60

accaaatccg cagcaaaatc aggagctggc gctctgaaga atggactggg ccaggtgaaa     120

caggcagggc agaaactgat tctctatatt ccgaaagact atcaagcttc caccggttcc     180

agcttgaatg atttggtgaa agcagcggaa gctctgggaa ttgaagtcca tcgtagcgag     240

aagaacggaa ccgcattggc gaaagagtta tttgggacca cggaaaaact gttaggcttt     300

agtgaacggg gtattgcact gtttgcacct cagtttgaca aactgcttaa caaaaaccag     360

aagttaagca aatcactggg tggcagctcc gaagccttgg gccagcgcct gaacaaaacc     420

cagacggcat tgagtgcact gcaaagtttt ctgggcacgg ctatcgccgg aatggatctg     480

gattctctcc tgcgtcggcg tcgcaacggt gaagatgtaa gtggatcgga gctggcgaaa     540

gctggtgtcg atctcgcggc acaattggtt gacaatattg cgtcggccac aggaacggtg     600

gatgcgtttg cggaacaact cggtaaactg ggcaacgcgt tatctaacac acgcttatcc     660

ggcttagcct ctaagctgaa taaccttccg gatctgtcct tggccggccc cggctttgat     720

gcggttagtg gcattcttag cgttgtgtcg gcgagtttca ttctgtccaa caaagatgca     780

gatgcgggca ccaaagccgc cgcgggcatt gaaatttcga cgaaaattct tggtaacatc     840

ggcaaggctg tgtctcagta tatcattgct caacgtgtgg ccgccgggtt aagcactaca     900

gcagcgacag gtggcctgat tggctctgtg gtagcactcg caattagtcc gctgtcattt     960

ctgaatgtcg cggataaatt tgaacgtgcc aaacaactgg aacagtatag cgaacgcttc    1020

aagaaatttg gttatgaagg ggatagtttg cttgcgagct tctaccgcga aaccggtgcc    1080

attgaagcgg ccctgaccac gatcaactcg gtgttgtcag ccgccagcgc cggggtcggt    1140

gcggcagcga cgggttcctt ggtaggtgca ccagttgcgg cgcttgtatc agccattact    1200

ggcatcatct ccggcattct ggatgccagc aagcaagcta tcttcgaacg tgtggcgacg    1260

aaactggcga acaaaattga tgaatgggag aaaaagcacg gcaaaaatta cttcgaaaac    1320

ggttatgatg cgcgccattc ggcgtttctg gaggatacat ttgaacttct gagccagtac    1380

aacaaagaat actctgtgga gcgtgtcgta gcgatcaccc agcaacggtg ggatgttaac    1440

attggtgaac tggccggcat tacccgcaaa ggcgctgacg cgaaatcagg caaagcatac    1500

gtcgacttct ttgaagaggg gaaactgttg gaaaaggacc cggaccgttt tgataagaaa    1560

gtctttgatc cgctggaagg gaaaattgat ctgtcctcta tcaacaaaac tacgttgctg    1620

aaattcatca cgcccgtttt cacagcgggt gaggaaattc gcgagcgtaa gcagactggg    1680

gcgtatgaat acatgaccga actgttcgtc aaaggtaaag agaaatgggt ggtgaccggc    1740

gttcagtcgc ataacgccat ctacgattat accaacttaa tccagctggc gattgacaaa    1800

aaaggtgaaa agcgtcaggt gaccattgag agccatctgg gcgagaagaa tgaccgcatc    1860

tatttaagca gtggtagttc gattgtctat gctggcaatg ggcatgatgt cgcctattat    1920

gacaaaaccg atacgggcta tttgaccttt gacggtcaga gcgcccagaa agcaggcgaa    1980

tatatcgtaa ccaaagaact gaaagctgat gtgaaagtcc tgaaagaggt tgtgaaaacg    2040

caagatatta gcgttggcgc ccgctcggag aaattagaat atcgcgatta tgagctgagc    2100

ccattcgaac tcggtaacgg cattcgcgcg aaagatgaac tccactctgt ggaagaaatt    2160

attggttcta atcgtaaaga taaattcttc gggtcacgct ttaccgatat ctttcatggc    2220

gcgaaagggg acgatgaaat ctatggcaac gacggccacg acattctgta tggtgatgat    2280

gggaacgacg ttatccatgg aggggatggc aatgatcatc tggttggcgg caatggaaat    2340

gaccgtctta tcggcggcaa aggtaataac tttcttaacg gtggtgatgg ggacgacgaa    2400

cttcaggtct ttgaaggtca gtataacgta ctgctgggcg gtgctgggaa cgacatcctt    2460

tatggtagtg atggtactaa tctgttcgac ggcggcgtgg gtaacgacaa gatctacgga    2520

gggttgggta aggacattta ccgctactcg aaagaatacg gccggcacat cattattgag    2580

aaaggcggag atgacgatac cctcctgctg tcggatttaa gctttaaaga cgtgggcttc    2640

attcgcatcg gggatgatct gttagtgaac aaacgcattg gaggcacgct gtactaccac    2700

gaagattaca atggcaatgc tctgaccatt aaggactggt tcaaagaagg caaggaaggt    2760

caaaacaata agatcgagaa aatcgtagat aaagacggcg cctatgttct cagccagtat    2820

ctcactgaac tgactgcacc tggccgcggt attaattact tcaatggtct ggaagagaaa    2880

ctgtactatg gcgagggtta taacgccctg ccacaactgc gcaaagacat tgaacagatt    2940

atcagctcta ccggggcgct gactggtgag catggtcaag ttctcgttgg tgcgggtggt    3000

ccgctggcgt actcaaattc gccgaatagc atcccgaatg ccttttccaa ttacctgacc    3060

cagtcagcat aagagctc                                                  3078


<210> 11
<211> 2880
<212> DNA
<213> Actonbacillus pleuropneumoniae


<220> 
<223> ApxIIA S148A K557A K686A

<400> 11
ggatccagca aaattaccct ctcatccctc aaatcaagcc tgcagcaggg gctgaagaac      60

ggcaagaaca agctgaatca ggcgggtacg actctgaaga atggcttaac gcaaacaggg     120

cactcattac agaacggtgc gaaaaaactg atcctgtaca tcccgcaagg ttacgactcg     180

gggcagggca atggggtcca agatcttgtt aaagcggcca atgacctggg aatcgaagtg     240

tggcgtgaag aacggagcaa cctggacatt gcgaaaacca gctttgatac gacccagaaa     300

atcctgggtt tcaccgaccg cggaattgta ctgtttgccc cgcaacttga taaccttctg     360

aaaaaaaacc cgaaaattgg aaatacattg ggttcggcaa gctctatcag tcagaacatt     420

ggcaaagcta acactgtgtt aggtggtatt cagtctattc tggggtcggt cttgtccggc     480

gttaacctga atgaactgct tcaaaacaaa gatccaaatc aactggaact ggccaaagcg     540

ggcctggagt taacgaacga gttggtagga aacattgcct cctctgtgca gacggtggat     600

gcatttgctg aacagattag caaattaggt tctcatctgc aaaacgtcaa aggactgggc     660

ggcctcagca acaaactgca gaatttaccg gatctgggca aagccagcct tgggttagat     720

attatctccg ggctcttgtc aggcgcgtca gccggtctta tcctggcgga caaagaagca     780

agcaccgaaa agaaagctgc cgcaggcgtc gagtttgcga accagattat tggaaatgtc     840

accaaagcgg tcagttccta catcctggcg cagcgtgtag cgtcgggcct gtcatctact     900

ggtccagttg ccgcgttgat tgcgtctacg gtcgccttag cggtttcgcc gctgtccttt     960

ctgaacgtgg cggataagtt caaacaagcg gatttgatca aaagctatag tgaacgcttt    1020

cagaaattag gttatgatgg tgaccgtctg ctggcggatt tccaccgcga aaccgggacc    1080

attgatgcct ctgtcactac catcaatacg gctttggcag cgatctccgg tggggtgggc    1140

gcggcgagcg ctggctcact ggtaggagcc cctgttgctc ttttggttgc aggcgttaca    1200

ggcctgatta cgaccatttt agagtatagc aaacaagcga tgtttgagca cgtcgcaaat    1260

aaggtacacg atcgtattgt agagtgggaa aagaaacata acaagaatta cttcgaacag    1320

gggtatgatt cacgccatct ggcagatctt caggacaaca tgaaatttct gatcaatctg    1380

aacaaagagc tgcaagcaga acgcgttgtg gcgattacac aacagcgttg ggataaccag    1440

attggcgatc tggcagcaat ctcgcgccgt acggacaaaa tttcttcggg taaagcctac    1500

gtggacgcct ttgaagaagg acagcatcag tcttatgatt cctcggtaca gctggacaac    1560

aaaaatggaa tcatcaacat tagtaatacc aaccgcaaga cccaaagtgt cctgtttcgc    1620

actcccctgc tcaccccggg cgaagagaac cgcgaacgca ttcaagaggg tgcaaactca    1680

tacattacca aattgcacat tcagcgtgtg gatagttgga ctgtcactga cggtgatgcg    1740

agcagcagtg ttgactttac taacgtggtt caacgcattg ccgtcaaatt cgacgacgct    1800

gggaatatta ttgaaagcaa agatacgaaa attatcgcta atttgggcgc tggtaatgat    1860

aatgtgttcg tgggttccag caccacggtt attgatggtg gcgatgggca tgaccgcgtg    1920

cattactcgc gtggcgaata tggcgccctt gtaatcgacg ccaccgcgga aacagagaaa    1980

ggtagctatt cggtgaaacg ctatgtgggt gattctaaag cgttacacga aaccattgcc    2040

acacatcaga ccaacgtggg cgctcgtgaa gagaaaatcg aatatcggcg tgaagatgat    2100

cgttttcata caggctacac cgtaacggac tctctcaaaa gcgttgaaga aattatcggc    2160

agccagttca atgatatctt caaaggctca caattcgacg atgtgttcca tggaggtaat    2220

ggggttgaca ccattgatgg caatgacggc gatgatcatc tgtttggcgg cgctggtgac    2280

gatgtgatcg atggtggtaa cggtaataat tttctcgtcg gcggtaccgg gaacgatatt    2340

atcagcggcg gtaaggacaa tgatatttat gtgcacaaga ctggtgatgg caacgactcc    2400

atcaccgata gcggtggcca ggataaactg gcatttagcg atgtgaacct caaagatctg    2460

acctttaaaa aagtggactc tagtctggaa atcattaacc agaagggcga gaaagtccgc    2520

attggtaact ggtttctgga agatgactta gctagcaccg tggcgaacta taaagccacg    2580

aatgatcgca aaattgaaga aatcatcggc aagggcggcg agcgtattac ttcggaacag    2640

gtggataaac tgatcaaaga gggtaacaat caaattagcg ccgaagcgct gtcgaaagta    2700

gtgaacgact acaatacgag taaagatcgc cagaatgttt ccaactcgct ggccaagctg    2760

atctccagtg ttggctcatt caccagttcg agtgatttcc ggaacaatct cggtacgtat    2820

gttccttctt ccatcgacgt tagtaacaat attcagttgg cacgcgcagc ataagagctc    2880


<210> 12
<211> 3168
<212> DNA
<213> Actonbacillus pleuropneumoniae


<220> 
<223> ApxIIIA K571A K702A

<400> 12
ggatccagca catggagcag tatgcttgcg gacttgaaga aacgggcgga agaagcgaaa       60

cgccaagcga agaaaggata tgatgtgact aagaacggcc tgcagtacgg tgtatcgcaa      120

gctaaactgc aggccctggc ggccgggaaa gcggttcaga agtatggtaa caaactcgtc      180

cttgtgattc ccaaagaata cgatggcagc gtgggtaatg gtttctttga tctggttaaa      240

gcggccgagg aactcggcat tcaggttaaa tatgtcaatc gtaacgagct tgaggttgcg      300

cacaaatccc tgggtactgc ggatcaattc ctcggcctca cggaacgtgg tctgactctg      360

ttcgccccac agctcgatca gttcttacag aagcacagca agatttccaa tgtagtcggg      420

agtagcactg gtgatgctgt cagcaaactt gctaaatcgc agactatcat ctcgggtatt      480

cagtcagtgc tgggcacggt tctggcgggc atcaacctga acgaagctat tatcagtggt      540

ggttccgaat tagaactggc cgaagccggg gtgagtcttg cctcagaact ggtgagcaat      600

attgcgaaag gcacgaccac gattgatgct ttcaccaccc aaatccagaa ttttgggaaa      660

ttggtggaaa atgcgaaagg cctgggaggc gtaggccgcc agctgcaaaa catttcgggg      720

agcgccctct ctaaaaccgg cctggggtta gacattatct cttcgctgct gtcaggagtt      780

accgcgagct ttgccctggc aaacaagaat gccagcacgt ctactaaagt ggcagcgggt      840

tttgaactct caaatcaggt cattggtggc attactaaag ccgtatcgag ttacattctg      900

gcgcaacgtc tggctgcagg cctgtctacg accggccctg cggcagcgct gattgcgtct      960

tctatctcgt tggcgatttc gcctctggcg tttctgcgtg ttgctgataa tttcaatcgg     1020

tccaaagaaa tcggggagtt tgcagagcgt tttaagaagc tgggatatga cggtgataaa     1080

ttgctgagcg aattttacca tgaggcgggc actatcgacg cctccattac caccatttca     1140

accgcactga gcgcgatcgc agcaggaacg gcggcagctt cggcgggtgc attagtggga     1200

gcaccgatta ccctgttggt taccgggatt accgggttaa ttagcggcat tctggaattc     1260

agtaaacaac cgatgctgga tcatgtcgca agcaaaatcg gtaacaaaat tgacgaatgg     1320

gaaaaaaaat acggcaaaaa ctacttcgaa aatggctatg acgcgcgcca caaggccttt     1380

ctcgaagatt cattttcact gttgagcagc ttcaataagc agtatgaaac ggaacgtgcc     1440

gttttgatca cgcagcagcg ttgggatgaa tacattggcg aattagccgg aattacgggc     1500

aaaggtgaca aactgtcctc tggcaaagcc tatgtggact acttccaaga aggtaaactg     1560

ctcgaaaaaa aaccagatga tttctcgaaa gtggtctttg atcccacgaa gggtgagatc     1620

gacatcagca attctcagac atctaccctg ctgaagtttg ttacgccgtt gttgaccccg     1680

ggtacggaat cacgcgaacg cacacagaca ggcgcctatg agtacattac caaattagtg     1740

gtaaaaggca aagataaatg ggtagttaac ggcgtgaaag acaaaggagc agtgtatgac     1800

tacacgaact taattcagca tgcacacatt agctcctccg tcgcacgcgg tgaagagtac     1860

cgtgaagttc gccttgtaag tcatttgggt aacggaaatg ataaagtgtt cctcgccgca     1920

ggaagtgcgg aaattcatgc tggcgaaggt catgacgtcg tgtactatga taaaaccgat     1980

acaggccttc tggttattga cggcactaaa gctaccgaac aggggcgtta cagtgtgacc     2040

cgcgaacttt ccggtgctac caaaatctta cgcgaagtca tcaagaatca gaaaagtgcc     2100

gtaggcgcgc gtgaagagac actggaatat cgggactatg agttaacgca aagcgggaac     2160

agtaatttaa aggctcacga tgaactgcat tccgtagagg agattatcgg cagcaaccag     2220

cgtgatgaat ttaaaggcag caaattccgc gatatcttcc atggtgccga cggtgacgac     2280

cttcttaacg ggaatgatgg ggatgacatc ctgtatggtg acaagggcaa tgatgaactg     2340

cgtggcgata acggcaatga ccagctgtac ggtggtgagg ggaatgacaa attgttaggt     2400

ggcaacggaa acaactatct gtccggcggc gacggtaatg atgagttgca agttcttggc     2460

aatggcttta acgtgctgcg cggcggcaaa ggtgatgata aactgtatgg tagttcaggc     2520

tcagatttgc tggatggcgg ggaaggtaac gattatctgg aaggcggtga tggctctgat     2580

ttctatgtgt atcgctcgac ctctggcaac cataccattt atgaccaggg aaaatcctcg     2640

gatctggata aactctatct gtccgatttt agttttgatc gcctgctggt cgagaaagtt     2700

gatgacaatc tggttctgcg ctcgaacgaa tctagccaca acaatggtgt gcttaccatc     2760

aaagattggt ttaaagaggg taacaaatac aatcacaaaa tcgagcaaat tgtggataag     2820

aatggtcgca agctgaccgc cgagaattta gggacttact ttaaaaatgc cccgaaagcg     2880

gataatctcc tgaactatgc aaccaaggag gaccagaacg aatcgaacct gtcctctctg     2940

aaaacggaac tgagcaaaat cattaccaac gcaggcaact ttggcgtggc taaacaaggg     3000

aacacaggga tcaataccgc ggcgctgaac aacgaagtca acaaaattat cagtagcgcc     3060

aacacatttg cgacctctca actgggtggt agcggtatgg gaaccttgcc gtcaacgaat     3120

gtcaacagca tgatgctggg caacttagcc cgcgctgcat aagagctc                  3168


<210> 13
<211> 527
<212> DNA
<213> Actonbacillus pleuropneumoniae


<220> 
<223> ApxIC

<400> 13
tcatgagtaa aaaaattaat ggatttgagg ttttaggaga ggtggcatgg ttatgggcaa      60

gttctccttt acatcgaaag tggccgcttt ctttgttagc aattaatgtg ctacctgcga     120

ttgagagtaa tcaatatgtt ttgttaaagc gtgacggttt tcctattgca ttttgtagct     180

gggcaaattt gaatttggaa aatgaaatta aataccttga tgatgttgcc tcgctagttg     240

cggatgattg gacttccggc gatcgtcgat ggtttataga ttggatagca ccgttcggag     300

acagtgccgc attatacaaa catatgcgag ataacttccc gaatgagctg tttagggcta     360

ttcgagttga tccggactct cgagtaggga aaatttcaga atttcatgga ggaaaaattg     420

ataagaaact ggcaagtaaa atttttcaac aatatcactt tgaattaatg agtgagctaa     480

aaaataaaca aaattttaaa ttttcattag taaatagcta aagatct                   527


<210> 14
<211> 488
<212> DNA
<213> Actonbacillus pleuropneumoniae


<220> 
<223> ApxIIC

<400> 14
acatgttaaa aaatgatttt aacgtattgg gacaaattgc ttggttatgg gcaaattctc      60

caatgcaccg aaattggtca gtttcactgt taatgaagaa tgttattcct gcaattgaaa     120

atgaccaata tttgttacta gttgatgatg gttttcctat tgcatattgc agttgggcca     180

aattaactct agagagtgag gctcgctatg taaaggacac caattcatta aaaatagatg     240

attggaatgc aggagatcgt atatggatca ttgattggat tgccccattc ggggattcat     300

ctctattgta taaacatatg agacaacgtt ttccatacga tattggaagg gcaattagaa     360

tctatcctag caaaaaagat actggaaaaa tcatatattt aaaaggagga aaaataacaa     420

aaaaagtagc tgaaaagaca tttcttcagt atgagcaaga gttaataaca gctctacaat     480

aaagatct                                                              488


<210> 15
<211> 530
<212> DNA
<213> Actonbacillus pleuropneumoniae


<220> 
<223> ApxIIIC

<400> 15
tcatgagtta taaaaatgtt aaaaatttaa cagatgattt tacaacttta gggcatatcg      60

cttggttgtg ggctaattct ccgttacata aggagtggtc tatctctttg tttactaaga     120

atattttgcc agccattcaa catgatcaat atattttact tatgcgagat gagttccctg     180

tagcgttttg tagttgggca aatttaacgt taactaatga agtgaagtat gtacgtgatg     240

tgacgtcatt gacttttgaa gattggaatt caggagaacg aaaatggttg atcgactgga     300

ttgcgccatt tggggataac aatacgcttt atagatatat gcgtaaaaaa tttcctaatg     360

aagtattccg ggccattcga gtatatcctg gttctacaga agcgaaaatc attcatgttc     420

aaggaggaca aattaataaa tttacagcta aaaaattaat acaacaatat caggaagaac     480

ttattcaagt tcttaacaat cacaaaaaaa ttgtaagagg ataaagatct                530


<210> 16
<211> 639
<212> DNA
<213> Actonbacillus pleuropneumoniae


<220> 
<223> ApxIC with promoter/lac-operator and RBS

<400> 16
tctagaaaat cataaaaaat ttatttgctt tgtgagcgga taacaattat aatagattca      60

attgtgagcg gataacaatt tcacacagaa ttcattaaag aggagaaatt aaccatgagt     120

aaaaaaatta atggatttga ggttttagga gaggtggcat ggttatgggc aagttctcct     180

ttacatcgaa agtggccgct ttctttgtta gcaattaatg tgctacctgc gattgagagt     240

aatcaatatg ttttgttaaa gcgtgacggt tttcctattg cattttgtag ctgggcaaat     300

ttgaatttgg aaaatgaaat taaatacctt gatgatgttg cctcgctagt tgcggatgat     360

tggacttccg gcgatcgtcg atggtttata gattggatag caccgttcgg agacagtgcc     420

gcattataca aacatatgcg agataacttc ccgaatgagc tgtttagggc tattcgagtt     480

gatccggact ctcgagtagg gaaaatttca gaatttcatg gaggaaaaat tgataagaaa     540

ctggcaagta aaatttttca acaatatcac tttgaattaa tgagtgagct aaaaaataaa     600

caaaatttta aattttcatt agtaaatagc taatcgcga                            639


<210> 17
<211> 600
<212> DNA
<213> Actonbacillus pleuropneumoniae


<220> 
<223> ApxIIC with promoter/lac-operator and RBS

<400> 17
atcgataaat cataaaaaat ttatttgctt tgtgagcgga taacaattat aatagattca      60

attgtgagcg gataacaatt tcacacagaa ttcattaaag aggagaaatt aaccatgtta     120

aaaaatgatt ttaacgtatt gggacaaatt gcttggttat gggcaaattc tccaatgcac     180

cgaaattggt cagtttcact gttaatgaag aatgttattc ctgcaattga aaatgaccaa     240

tatttgttac tagttgatga tggttttcct attgcatatt gcagttgggc caaattaact     300

ctagagagtg aggctcgcta tgtaaaggac accaattcat taaaaataga tgattggaat     360

gcaggagatc gtatatggat cattgattgg attgccccat tcggggattc atctctattg     420

tataaacata tgagacaacg ttttccatac gatattggaa gggcaattag aatctatcct     480

agcaaaaaag atactggaaa aatcatatat ttaaaaggag gaaaaataac aaaaaaagta     540

gctgaaaaga catttcttca gtatgagcaa gagttaataa cagctctaca ataatcgcga     600


<210> 18
<211> 642
<212> DNA
<213> Actonbacillus pleuropneumoniae


<220> 
<223> ApxIIIC with promoter/lac-operator and RBS

<400> 18
tctagaaaat cataaaaaat ttatttgctt tgtgagcgga taacaattat aatagattca      60

attgtgagcg gataacaatt tcacacagaa ttcattaaag aggagaaatt aaccatgagt     120

tataaaaatg ttaaaaattt aacagatgat tttacaactt tagggcatat cgcttggttg     180

tgggctaatt ctccgttaca taaggagtgg tctatctctt tgtttactaa gaatattttg     240

ccagccattc aacatgatca atatatttta cttatgcgag atgagttccc tgtagcgttt     300

tgtagttggg caaatttaac gttaactaat gaagtgaagt atgtacgtga tgtgacgtca     360

ttgacttttg aagattggaa ttcaggagaa cgaaaatggt tgatcgattg gattgcgcca     420

tttggggata acaatacgct ttatagatat atgcgtaaaa aatttcctaa tgaagtattc     480

cgggccattc gagtatatcc tggttctaca gaagcgaaaa tcattcatgt tcaaggagga     540

caaattaata aatttacagc taaaaaatta atacaacaat atcaggaaga acttattcaa     600

gttcttaaca atcacaaaaa aattgtaaga ggataatcgc ga                        642


<210> 19
<211> 2446
<212> DNA
<213> Plasmid pEX-A258


<220> 
<223> pEX-A258

<400> 19
gtggcagctc tagagctagc gaattctttg gtgaaattgt tatccgctca caattccaca      60

caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact     120

cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct     180

gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc     240

ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca     300

ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg     360

agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca     420

taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa     480

cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc     540

tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc     600

gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct     660

gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg     720

tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag     780

gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta     840

cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg     900

aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt     960

tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt    1020

ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag    1080

attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat    1140

ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc    1200

tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat    1260

aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgcgaacc    1320

acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag    1380

aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag    1440

agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt    1500

ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg    1560

agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt    1620

tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc    1680

tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc    1740

attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa    1800

taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg    1860

aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc    1920

caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag    1980

gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt    2040

cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt    2100

tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc    2160

acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac    2220

gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct    2280

cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg    2340

cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat    2400

tgtactgaga gtttggcaat tggtcgacct cgagggcgcg cccgta                   2446


<210> 20
<211> 4751
<212> DNA
<213> Plasmid pQE-80L


<220> 
<223> pQE-80L

<400> 20
ctcgagaaat cataaaaaat ttatttgctt tgtgagcgga taacaattat aatagattca       60

attgtgagcg gataacaatt tcacacagaa ttcattaaag aggagaaatt aactatgaga      120

ggatcgcatc accatcacca tcacggatcc gcatgcgagc tcggtacccc gggtcgacct      180

gcagccaagc ttaattagct gagcttggac tcctgttgat agatccagta atgacctcag      240

aactccatct ggatttgttc agaacgctcg gttgccgccg ggcgtttttt attggtgaga      300

atccaagcta gcttggcgag attttcagga gctaaggaag ctaaaatgga gaaaaaaatc      360

actggatata ccaccgttga tatatcccaa tggcatcgta aagaacattt tgaggcattt      420

cagtcagttg ctcaatgtac ctataaccag accgttcagc tggatattac ggccttttta      480

aagaccgtaa agaaaaataa gcacaagttt tatccggcct ttattcacat tcttgcccgc      540

ctgatgaatg ctcatccgga atttcgtatg gcaatgaaag acggtgagct ggtgatatgg      600

gatagtgttc acccttgtta caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc      660

tggagtgaat accacgacga tttccggcag tttctacaca tatattcgca agatgtggcg      720

tgttacggtg aaaacctggc ctatttccct aaagggttta ttgagaatat gtttttcgtc      780

tcagccaatc cctgggtgag tttcaccagt tttgatttaa acgtggccaa tatggacaac      840

ttcttcgccc ccgttttcac catgggcaaa tattatacgc aaggcgacaa ggtgctgatg      900

ccgctggcga ttcaggttca tcatgccgtt tgtgatggct tccatgtcgg cagaatgctt      960

aatgaattac aacagtactg cgatgagtgg cagggcgggg cgtaattttt ttaaggcagt     1020

tattggtgcc cttaaacgcc tggggtaatg actctctagc ttgaggcatc aaataaaacg     1080

aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct     1140

cctgagtagg acaaatccgc cctctagatt acgtgcagtc gatgataagc tgtcaaacat     1200

gagaattgtg cctaatgagt gagctaactt acattaattg cgttgcgctc actgcccgct     1260

ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga     1320

ggcggtttgc gtattgggcg ccagggtggt ttttcttttc accagtgaga cgggcaacag     1380

ctgattgccc ttcaccgcct ggccctgaga gagttgcagc aagcggtcca cgctggtttg     1440

ccccagcagg cgaaaatcct gtttgatggt ggttaacggc gggatataac atgagctgtc     1500

ttcggtatcg tcgtatccca ctaccgagat atccgcacca acgcgcagcc cggactcggt     1560

aatggcgcgc attgcgccca gcgccatctg atcgttggca accagcatcg cagtgggaac     1620

gatgccctca ttcagcattt gcatggtttg ttgaaaaccg gacatggcac tccagtcgcc     1680

ttcccgttcc gctatcggct gaatttgatt gcgagtgaga tatttatgcc agccagccag     1740

acgcagacgc gccgagacag aacttaatgg gcccgctaac agcgcgattt gctggtgacc     1800

caatgcgacc agatgctcca cgcccagtcg cgtaccgtct tcatgggaga aaataatact     1860

gttgatgggt gtctggtcag agacatcaag aaataacgcc ggaacattag tgcaggcagc     1920

ttccacagca atggcatcct ggtcatccag cggatagtta atgatcagcc cactgacgcg     1980

ttgcgcgaga agattgtgca ccgccgcttt acaggcttcg acgccgcttc gttctaccat     2040

cgacaccacc acgctggcac ccagttgatc ggcgcgagat ttaatcgccg cgacaatttg     2100

cgacggcgcg tgcagggcca gactggaggt ggcaacgcca atcagcaacg actgtttgcc     2160

cgccagttgt tgtgccacgc ggttgggaat gtaattcagc tccgccatcg ccgcttccac     2220

tttttcccgc gttttcgcag aaacgtggct ggcctggttc accacgcggg aaacggtctg     2280

ataagagaca ccggcatact ctgcgacatc gtataacgtt actggtttca cattcaccac     2340

cctgaattga ctctcttccg ggcgctatca tgccataccg cgaaaggttt tgcaccattc     2400

gatggtgtcg gaatttcggg cagcgttggg tcctggccac gggtgcgcat gatctagagc     2460

tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg     2520

gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg     2580

ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat     2640

actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg     2700

aaataccgca cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc     2760

tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg     2820

cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag     2880

gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc     2940

gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag     3000

gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga     3060

ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc     3120

atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg     3180

tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt     3240

ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca     3300

gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca     3360

ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag     3420

ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca     3480

agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg     3540

ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa     3600

aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta     3660

tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag     3720

cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga     3780

tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac     3840

cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc     3900

ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta     3960

gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac     4020

gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat     4080

gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa     4140

gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg     4200

tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag     4260

aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc     4320

cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct     4380

caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat     4440

cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg     4500

ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc     4560

aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta     4620

tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg     4680

tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct     4740

ttcgtcttca c                                                          4751


<210> 21
<211> 3431
<212> DNA
<213> Plasmid pQE-60


<220> 
<223> pQE-60

<400> 21
ctcgagaaat cataaaaaat ttatttgctt tgtgagcgga taacaattat aatagattca       60

attgtgagcg gataacaatt tcacacagaa ttcattaaag aggagaaatt aaccatggga      120

ggatccagat ctcatcacca tcaccatcac taagcttaat tagctgagct tggactcctg      180

ttgatagatc cagtaatgac ctcagaactc catctggatt tgttcagaac gctcggttgc      240

cgccgggcgt tttttattgg tgagaatcca agctagcttg gcgagatttt caggagctaa      300

ggaagctaaa atggagaaaa aaatcactgg atataccacc gttgatatat cccaatggca      360

tcgtaaagaa cattttgagg catttcagtc agttgctcaa tgtacctata accagaccgt      420

tcagctggat attacggcct ttttaaagac cgtaaagaaa aataagcaca agttttatcc      480

ggcctttatt cacattcttg cccgcctgat gaatgctcat ccggaatttc gtatggcaat      540

gaaagacggt gagctggtga tatgggatag tgttcaccct tgttacaccg ttttccatga      600

gcaaactgaa acgttttcat cgctctggag tgaataccac gacgatttcc ggcagtttct      660

acacatatat tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg      720

gtttattgag aatatgtttt tcgtctcagc caatccctgg gtgagtttca ccagttttga      780

tttaaacgtg gccaatatgg acaacttctt cgcccccgtt ttcaccatgc atgggcaaat      840

attatacgca aggcgacaag gtgctgatgc cgctggcgat tcaggttcat catgccgtct      900

gtgatggctt ccatgtcggc agaatgctta atgaattaca acagtactgc gatgagtggc      960

agggcggggc gtaatttttt taaggcagtt attggtgccc ttaaacgcct ggggtaatga     1020

ctctctagct tgaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt     1080

tttatctgtt gtttgtcggt gaacgctctc ctgagtagga caaatccgcc gctctagagc     1140

tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg     1200

gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg     1260

ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat     1320

actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg     1380

aaataccgca cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc     1440

tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg     1500

cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag     1560

gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc     1620

gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag     1680

gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga     1740

ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc     1800

atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg     1860

tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt     1920

ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca     1980

gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca     2040

ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag     2100

ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca     2160

agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg     2220

ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa     2280

aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta     2340

tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag     2400

cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga     2460

tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac     2520

cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc     2580

ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta     2640

gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac     2700

gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat     2760

gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa     2820

gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg     2880

tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag     2940

aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc     3000

cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct     3060

caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat     3120

cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg     3180

ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc     3240

aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta     3300

tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg     3360

tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct     3420

ttcgtcttca c                                                          3431


<210> 22
<211> 4245
<212> DNA
<213> Plasmid pACYC184


<220> 
<223> pACYC184

<400> 22
gaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt       60

gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt      120

ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga      180

tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga      240

aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt      300

ggaacctctt acgtgccgat caacgtctca ttttcgccaa aagttggccc agggcttccc      360

ggtatcaaca gggacaccag gatttattta ttctgcgaag tgatcttccg tcacaggtat      420

ttattcggcg caaagtgcgt cgggtgatgc tgccaactta ctgatttagt gtatgatggt      480

gtttttgagg tgctccagtg gcttctgttt ctatcagctg tccctcctgt tcagctactg      540

acggggtggt gcgtaacggc aaaagcaccg ccggacatca gcgctagcgg agtgtatact      600

ggcttactat gttggcactg atgagggtgt cagtgaagtg cttcatgtgg caggagaaaa      660

aaggctgcac cggtgcgtca gcagaatatg tgatacagga tatattccgc ttcctcgctc      720

actgactcgc tacgctcggt cgttcgactg cggcgagcgg aaatggctta cgaacggggc      780

ggagatttcc tggaagatgc caggaagata cttaacaggg aagtgagagg gccgcggcaa      840

agccgttttt ccataggctc cgcccccctg acaagcatca cgaaatctga cgctcaaatc      900

agtggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggcggctccc      960

tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt cattccgctg ttatggccgc     1020

gtttgtctca ttccacgcct gacactcagt tccgggtagg cagttcgctc caagctggac     1080

tgtatgcacg aaccccccgt tcagtccgac cgctgcgcct tatccggtaa ctatcgtctt     1140

gagtccaacc cggaaagaca tgcaaaagca ccactggcag cagccactgg taattgattt     1200

agaggagtta gtcttgaagt catgcgccgg ttaaggctaa actgaaagga caagttttgg     1260

tgactgcgct cctccaagcc agttacctcg gttcaaagag ttggtagctc agagaacctt     1320

cgaaaaaccg ccctgcaagg cggttttttc gttttcagag caagagatta cgcgcagacc     1380

aaaacgatct caagaagatc atcttattaa tcagataaaa tatttctaga tttcagtgca     1440

atttatctct tcaaatgtag cacctgaagt cagccccata cgatataagt tgtaattctc     1500

atgtttgaca gcttatcatc gataagcttt aatgcggtag tttatcacag ttaaattgct     1560

aacgcagtca ggcaccgtgt atgaaatcta acaatgcgct catcgtcatc ctcggcaccg     1620

tcaccctgga tgctgtaggc ataggcttgg ttatgccggt actgccgggc ctcttgcggg     1680

atatcgtcca ttccgacagc atcgccagtc actatggcgt gctgctagcg ctatatgcgt     1740

tgatgcaatt tctatgcgca cccgttctcg gagcactgtc cgaccgcttt ggccgccgcc     1800

cagtcctgct cgcttcgcta cttggagcca ctatcgacta cgcgatcatg gcgaccacac     1860

ccgtcctgtg gatcctctac gccggacgca tcgtggccgg catcaccggc gccacaggtg     1920

cggttgctgg cgcctatatc gccgacatca ccgatgggga agatcgggct cgccacttcg     1980

ggctcatgag cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc gggggactgt     2040

tgggcgccat ctccttgcat gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc     2100

tactactggg ctgcttccta atgcaggagt cgcataaggg agagcgtcga ccgatgccct     2160

tgagagcctt caacccagtc agctccttcc ggtgggcgcg gggcatgact atcgtcgccg     2220

cacttatgac tgtcttcttt atcatgcaac tcgtaggaca ggtgccggca gcgctctggg     2280

tcattttcgg cgaggaccgc tttcgctgga gcgcgacgat gatcggcctg tcgcttgcgg     2340

tattcggaat cttgcacgcc ctcgctcaag ccttcgtcac tggtcccgcc accaaacgtt     2400

tcggcgagaa gcaggccatt atcgccggca tggcggccga cgcgctgggc tacgtcttgc     2460

tggcgttcgc gacgcgaggc tggatggcct tccccattat gattcttctc gcttccggcg     2520

gcatcgggat gcccgcgttg caggccatgc tgtccaggca ggtagatgac gaccatcagg     2580

gacagcttca aggatcgctc gcggctctta ccagcctaac ttcgatcact ggaccgctga     2640

tcgtcacggc gatttatgcc gcctcggcga gcacatggaa cgggttggca tggattgtag     2700

gcgccgccct ataccttgtc tgcctccccg cgttgcgtcg cggtgcatgg agccgggcca     2760

cctcgacctg aatggaagcc ggcggcacct cgctaacgga ttcaccactc caagaattgg     2820

agccaatcaa ttcttgcgga gaactgtgaa tgcgcaaacc aacccttggc agaacatatc     2880

catcgcgtcc gccatctcca gcagccgcac gcggcgcatc tcgggcagcg ttgggtcctg     2940

gccacgggtg cgcatgatcg tgctcctgtc gttgaggacc cggctaggct ggcggggttg     3000

ccttactggt tagcagaatg aatcaccgat acgcgagcga acgtgaagcg actgctgctg     3060

caaaacgtct gcgacctgag caacaacatg aatggtcttc ggtttccgtg tttcgtaaag     3120

tctggaaacg cggaagtccc ctacgtgctg ctgaagttgc ccgcaacaga gagtggaacc     3180

aaccggtgat accacgatac tatgactgag agtcaacgcc atgagcggcc tcatttctta     3240

ttctgagtta caacagtccg caccgctgtc cggtagctcc ttccggtggg cgcggggcat     3300

gactatcgtc gccgcactta tgactgtctt ctttatcatg caactcgtag gacaggtgcc     3360

ggcagcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc     3420

gccctgcacc attatgttcc ggatctgcat cgcaggatgc tgctggctac cctgtggaac     3480

acctacatct gtattaacga agcgctaacc gtttttatca ggctctggga ggcagaataa     3540

atgatcatat cgtcaattat tacctccacg gggagagcct gagcaaactg gcctcaggca     3600

tttgagaagc acacggtcac actgcttccg gtagtcaata aaccggtaaa ccagcaatag     3660

acataagcgg ctatttaacg accctgccct gaaccgacga ccgggtcgaa tttgctttcg     3720

aatttctgcc attcatccgc ttattatcac ttattcaggc gtagcaccag gcgtttaagg     3780

gcaccaataa ctgccttaaa aaaattacgc cccgccctgc cactcatcgc agtactgttg     3840

taattcatta agcattctgc cgacatggaa gccatcacag acggcatgat gaacctgaat     3900

cgccagcggc atcagcacct tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg     3960

ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg     4020

attggctgag acgaaaaaca tattctcaat aaacccttta gggaaatagg ccaggttttc     4080

accgtaacac gccacatctt gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta     4140

ttcactccag agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg     4200

aacactatcc catatcacca gctcaccgtc tttcattgcc atacg                     4245


<210> 23
<211> 33
<212> DNA
<213> Artificial Sequence


<220> 
<223> Primer

<400> 23
gcatcatgag taaaaaaatt aatggatttg agg                                  33


<210> 24
<211> 35
<212> DNA
<213> Artificial Sequence


<220> 
<223> Primer

<400> 24
ggtagatctt tagctattta ctaatgaaaa tttaa                                35


<210> 25
<211> 33
<212> DNA
<213> Artificial Sequence


<220> 
<223> Primer

<400> 25
gcaacatgtt aaaaaatgat tttaacgtat tgg                                  33


<210> 26
<211> 32
<212> DNA
<213> Artificial Sequence


<220> 
<223> Primer

<400> 26
ggtagatctt tattgtagag ctgttattaa ct                                   32


<210> 27
<211> 35
<212> DNA
<213> Artificial Sequence


<220> 
<223> Primer

<400> 27
gcatcatgag ttataaaaat gttaaaaatt taaca                                35


<210> 28
<211> 33
<212> DNA
<213> Artificial Sequence


<220> 
<223> Primer

<400> 28
ggtagatctt tatcctctta caattttttt gtg                                  33


<210> 29
<211> 34
<212> DNA
<213> Artificial Sequence


<220> 
<223> Primer

<400> 29
gcatctagaa aatcataaaa aatttatttg cttt                                 34


<210> 30
<211> 34
<212> DNA
<213> Artificial Sequence


<220> 
<223> Primer

<400> 30
agctcgcgat tagctattta ctaatgaaaa ttta                                 34


<210> 31
<211> 34
<212> DNA
<213> Artificial Sequence


<220> 
<223> Primer

<400> 31
gcaatcgata aatcataaaa aatttatttg cttt                                 34


<210> 32
<211> 32
<212> DNA
<213> Artificial Sequence


<220> 
<223> Primer

<400> 32
agctcgcgat tattgtagag ctgttattaa ct                                   32


<210> 33
<211> 34
<212> DNA
<213> Artificial Sequence


<220> 
<223> Primer

<400> 33
gcatctagaa aatcataaaa aatttatttg cttt                                 34


<210> 34
<211> 32
<212> DNA
<213> Artificial Sequence


<220> 
<223> Primer

<400> 34
agctcgcgat tatcctctta caattttttt gt                                   32


