                         SEQUENCE LISTING

<110>  VIB VZW
       KATHOLIEKE UNIVERSITEIT LEUVEN, K.U.LEUVEN R&D
 
<120>  MEANS AND METHODS TO OVERRULE GLUCOSE-MEDIATED REPRESSION OF 
       RESPIRATION IN YEAST

<130>  KV/Respi/649

<150>  GB 1901262.4
<151>  2019-01-30

<160>  17    

<170>  PatentIn version 3.5

<210>  1
<211>  1458
<212>  DNA
<213>  Saccharomyces cerevisiae

<400>  1
atgtctggcc aaaagggcga gatcgtggtt tatacaaaag agttagaaac tacccccgag       60

cttttaccaa accatgaagt tttcaagatt aggattggcc aaaagctatt tgagatctca      120

ggtgctacat tgaattcgga tgcgcccaat tttttcaccc aattcttcaa tacgcacgat      180

aaaaatacga ttctatttgt tgatagatcg gaagacgtgt tcatcataat atacagacat      240

ttacaaggtt attttcccga tattaagaac gaagtacaat ttacctgtct tttcgcggac      300

gcattatatt ttcagttacc caagttagtt aaactcataa aagaatacga ctaccacttc      360

actaatattg gaggtgttcc ctttaaggta ccaaaatcat tattccatga agaagggaac      420

cggttgaact attttgaaac gatttctcgc atatcttatg aggaaattga gaagtgggag      480

tcaaataaga agccgggctt tccaccttta ttaccaccct catatattgc aaggtcccca      540

gaatttttcc gagatatatt atctttactg ggcggttcca aattagaact gtctgaggaa      600

agaaccgcat ctctgataaa agagtgtcgt tattaccgtc tcaacaggtt ggagcaggaa      660

ttagtgcggg caaaaattat atataatccc ttaactaatt gtcaagaagt ttgcatagct      720

cttgatagcg tctccaagaa gggtgtaacc attgaaaggt taacatcact tcataccgga      780

aaccaatctt tagcggtaag cagttgtctc aatggaactg agggtgaaaa agccgctact      840

ggatttcata aaaccgagac tgattcaggc aacaatgatg aatatgaacc tccaacaaaa      900

aaggtaaaac attgtattga aagacactgg agcatgttaa aataccaaag gccttatatc      960

gatacagttt cgcacgatct tattttccaa ttacactcta accagtgcaa aataatattc     1020

aacaaaaaga ataagactgt tcatgtagac ctatccagag aggctgcaat gttgtttgaa     1080

aacaagttct cggatgtttt attggaaaac ccagatttta aaattgatct gtccgaatat     1140

aaggtcaaac ttcgtgacag tcaaatgcaa gttgaatccc acttaattat tcctgcatgt     1200

gtctccattt gtgacttgac ggtaaatggc gcaaaatgct gtaatatttt ctcattagtt     1260

aacgactcca aatgtaaagg gagggtcttg gattgcacca atttgaaagt tttgaattgc     1320

gttcatggtt tgaaactaca cttatcgaaa tccatgtgga agctaggtac aaataatgga     1380

agaatcattc tcgttgctgt aaaagccgaa accttctctg ggacgaagga atattgcaaa     1440

atgattgatt ttctgtga                                                   1458


<210>  2
<211>  485
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  2

Met Ser Gly Gln Lys Gly Glu Ile Val Val Tyr Thr Lys Glu Leu Glu 
1               5                   10                  15      


Thr Thr Pro Glu Leu Leu Pro Asn His Glu Val Phe Lys Ile Arg Ile 
            20                  25                  30          


Gly Gln Lys Leu Phe Glu Ile Ser Gly Ala Thr Leu Asn Ser Asp Ala 
        35                  40                  45              


Pro Asn Phe Phe Thr Gln Phe Phe Asn Thr His Asp Lys Asn Thr Ile 
    50                  55                  60                  


Leu Phe Val Asp Arg Ser Glu Asp Val Phe Ile Ile Ile Tyr Arg His 
65                  70                  75                  80  


Leu Gln Gly Tyr Phe Pro Asp Ile Lys Asn Glu Val Gln Phe Thr Cys 
                85                  90                  95      


Leu Phe Ala Asp Ala Leu Tyr Phe Gln Leu Pro Lys Leu Val Lys Leu 
            100                 105                 110         


Ile Lys Glu Tyr Asp Tyr His Phe Thr Asn Ile Gly Gly Val Pro Phe 
        115                 120                 125             


Lys Val Pro Lys Ser Leu Phe His Glu Glu Gly Asn Arg Leu Asn Tyr 
    130                 135                 140                 


Phe Glu Thr Ile Ser Arg Ile Ser Tyr Glu Glu Ile Glu Lys Trp Glu 
145                 150                 155                 160 


Ser Asn Lys Lys Pro Gly Phe Pro Pro Leu Leu Pro Pro Ser Tyr Ile 
                165                 170                 175     


Ala Arg Ser Pro Glu Phe Phe Arg Asp Ile Leu Ser Leu Leu Gly Gly 
            180                 185                 190         


Ser Lys Leu Glu Leu Ser Glu Glu Arg Thr Ala Ser Leu Ile Lys Glu 
        195                 200                 205             


Cys Arg Tyr Tyr Arg Leu Asn Arg Leu Glu Gln Glu Leu Val Arg Ala 
    210                 215                 220                 


Lys Ile Ile Tyr Asn Pro Leu Thr Asn Cys Gln Glu Val Cys Ile Ala 
225                 230                 235                 240 


Leu Asp Ser Val Ser Lys Lys Gly Val Thr Ile Glu Arg Leu Thr Ser 
                245                 250                 255     


Leu His Thr Gly Asn Gln Ser Leu Ala Val Ser Ser Cys Leu Asn Gly 
            260                 265                 270         


Thr Glu Gly Glu Lys Ala Ala Thr Gly Phe His Lys Thr Glu Thr Asp 
        275                 280                 285             


Ser Gly Asn Asn Asp Glu Tyr Glu Pro Pro Thr Lys Lys Val Lys His 
    290                 295                 300                 


Cys Ile Glu Arg His Trp Ser Met Leu Lys Tyr Gln Arg Pro Tyr Ile 
305                 310                 315                 320 


Asp Thr Val Ser His Asp Leu Ile Phe Gln Leu His Ser Asn Gln Cys 
                325                 330                 335     


Lys Ile Ile Phe Asn Lys Lys Asn Lys Thr Val His Val Asp Leu Ser 
            340                 345                 350         


Arg Glu Ala Ala Met Leu Phe Glu Asn Lys Phe Ser Asp Val Leu Leu 
        355                 360                 365             


Glu Asn Pro Asp Phe Lys Ile Asp Leu Ser Glu Tyr Lys Val Lys Leu 
    370                 375                 380                 


Arg Asp Ser Gln Met Gln Val Glu Ser His Leu Ile Ile Pro Ala Cys 
385                 390                 395                 400 


Val Ser Ile Cys Asp Leu Thr Val Asn Gly Ala Lys Cys Cys Asn Ile 
                405                 410                 415     


Phe Ser Leu Val Asn Asp Ser Lys Cys Lys Gly Arg Val Leu Asp Cys 
            420                 425                 430         


Thr Asn Leu Lys Val Leu Asn Cys Val His Gly Leu Lys Leu His Leu 
        435                 440                 445             


Ser Lys Ser Met Trp Lys Leu Gly Thr Asn Asn Gly Arg Ile Ile Leu 
    450                 455                 460                 


Val Ala Val Lys Ala Glu Thr Phe Ser Gly Thr Lys Glu Tyr Cys Lys 
465                 470                 475                 480 


Met Ile Asp Phe Leu 
                485 


<210>  3
<211>  1458
<212>  DNA
<213>  Saccharomyces cerevisiae

<400>  3
atgtctggcc aaaagggcga gatcgtggtt tatacaaaag agttagaaac tacccccgag       60

cttttaccaa accatgaagt tttcaatatt aggattggcc aaaagctatt tgagatctca      120

ggtgcttcat tgaattcgga tgcgcccaat tttttcaccc aattcttcaa tacgcacgat      180

aaaaatacga ttctatttgt tgatagatcg gaagacgtgt tcatcataat atacagacat      240

ttacaaggtt attttcccga tattaaggac gaagtacaat ttacctgtct tttcgcggat      300

gcattatatt ttcagttacc caagttagtt aaactcataa aagaatacga ctaccacttc      360

actaatattg gaggtgttcc ctttaaggta ccaaaatcat tattccatga agaagggaac      420

cggttgaact attttgaaac gatttctcgc acatcttatg aggaaattga gaagtgggag      480

tcaaataaga agccgggctt tccaccttta ttaccaccct catatattgc aaggtcccca      540

gaatttttcc gagaaatatt atctttactg ggcggttctg aattagaact gtctgaggaa      600

agaaccgcat ctctgataaa agagtgtcgt tattaccgtc tcaacaggtt ggagcaggaa      660

ttagtgcggg caaaaattat atataatccc ttaactaatt gtcaagaagt ttgcatagct      720

ctcgatagcg tctccaagaa gggtgtaacc attgaaaggt taacatcact tcatactgga      780

aaccaatctt tagcggtagg cagttgtctc aatggaactg agggtgaaaa aaccgctact      840

ggatttcata aaaccgagac tgattcaggc aacaatgatg aatctgaacc tccaacaaaa      900

aaggtaaaac attgtattga aagacactgg agcatgttaa aataccaaag gccttatatc      960

gatacagttt cgcacgatct tattttccaa ttacactcta accagtgcaa aataatattc     1020

aacaaaaaga ataagactgt tcatgtagac ctatccagag aggctgcaat gttgtttgaa     1080

aacaagttct cggatgtttt attggaaaac ccagatttta aaattgatct gtccgaatat     1140

aaagtcaaac ttcgtgacag tcaaatgcaa gttgaatccc acttaattat tcctgcatgt     1200

gtctccattt gtgacttgac ggtaaatggc gcaaaatgct gtaatatttt ctcattagtt     1260

aacgactcca aatgtaaaga gagggtcttg gattgcacca atttgaaagt tttgaattgc     1320

gttcatggtt tgaaactaca cttatcgaaa tccatgtgga agctaggtac aaataatgga     1380

agaatcattc tcgttgctgt aaaagccgaa accttctctg ggacaaagga atattgcaaa     1440

atgattgatt ttctgtga                                                   1458


<210>  4
<211>  485
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  4

Met Ser Gly Gln Lys Gly Glu Ile Val Val Tyr Thr Lys Glu Leu Glu 
1               5                   10                  15      


Thr Thr Pro Glu Leu Leu Pro Asn His Glu Val Phe Asn Ile Arg Ile 
            20                  25                  30          


Gly Gln Lys Leu Phe Glu Ile Ser Gly Ala Ser Leu Asn Ser Asp Ala 
        35                  40                  45              


Pro Asn Phe Phe Thr Gln Phe Phe Asn Thr His Asp Lys Asn Thr Ile 
    50                  55                  60                  


Leu Phe Val Asp Arg Ser Glu Asp Val Phe Ile Ile Ile Tyr Arg His 
65                  70                  75                  80  


Leu Gln Gly Tyr Phe Pro Asp Ile Lys Asp Glu Val Gln Phe Thr Cys 
                85                  90                  95      


Leu Phe Ala Asp Ala Leu Tyr Phe Gln Leu Pro Lys Leu Val Lys Leu 
            100                 105                 110         


Ile Lys Glu Tyr Asp Tyr His Phe Thr Asn Ile Gly Gly Val Pro Phe 
        115                 120                 125             


Lys Val Pro Lys Ser Leu Phe His Glu Glu Gly Asn Arg Leu Asn Tyr 
    130                 135                 140                 


Phe Glu Thr Ile Ser Arg Thr Ser Tyr Glu Glu Ile Glu Lys Trp Glu 
145                 150                 155                 160 


Ser Asn Lys Lys Pro Gly Phe Pro Pro Leu Leu Pro Pro Ser Tyr Ile 
                165                 170                 175     


Ala Arg Ser Pro Glu Phe Phe Arg Glu Ile Leu Ser Leu Leu Gly Gly 
            180                 185                 190         


Ser Glu Leu Glu Leu Ser Glu Glu Arg Thr Ala Ser Leu Ile Lys Glu 
        195                 200                 205             


Cys Arg Tyr Tyr Arg Leu Asn Arg Leu Glu Gln Glu Leu Val Arg Ala 
    210                 215                 220                 


Lys Ile Ile Tyr Asn Pro Leu Thr Asn Cys Gln Glu Val Cys Ile Ala 
225                 230                 235                 240 


Leu Asp Ser Val Ser Lys Lys Gly Val Thr Ile Glu Arg Leu Thr Ser 
                245                 250                 255     


Leu His Thr Gly Asn Gln Ser Leu Ala Val Gly Ser Cys Leu Asn Gly 
            260                 265                 270         


Thr Glu Gly Glu Lys Thr Ala Thr Gly Phe His Lys Thr Glu Thr Asp 
        275                 280                 285             


Ser Gly Asn Asn Asp Glu Ser Glu Pro Pro Thr Lys Lys Val Lys His 
    290                 295                 300                 


Cys Ile Glu Arg His Trp Ser Met Leu Lys Tyr Gln Arg Pro Tyr Ile 
305                 310                 315                 320 


Asp Thr Val Ser His Asp Leu Ile Phe Gln Leu His Ser Asn Gln Cys 
                325                 330                 335     


Lys Ile Ile Phe Asn Lys Lys Asn Lys Thr Val His Val Asp Leu Ser 
            340                 345                 350         


Arg Glu Ala Ala Met Leu Phe Glu Asn Lys Phe Ser Asp Val Leu Leu 
        355                 360                 365             


Glu Asn Pro Asp Phe Lys Ile Asp Leu Ser Glu Tyr Lys Val Lys Leu 
    370                 375                 380                 


Arg Asp Ser Gln Met Gln Val Glu Ser His Leu Ile Ile Pro Ala Cys 
385                 390                 395                 400 


Val Ser Ile Cys Asp Leu Thr Val Asn Gly Ala Lys Cys Cys Asn Ile 
                405                 410                 415     


Phe Ser Leu Val Asn Asp Ser Lys Cys Lys Glu Arg Val Leu Asp Cys 
            420                 425                 430         


Thr Asn Leu Lys Val Leu Asn Cys Val His Gly Leu Lys Leu His Leu 
        435                 440                 445             


Ser Lys Ser Met Trp Lys Leu Gly Thr Asn Asn Gly Arg Ile Ile Leu 
    450                 455                 460                 


Val Ala Val Lys Ala Glu Thr Phe Ser Gly Thr Lys Glu Tyr Cys Lys 
465                 470                 475                 480 


Met Ile Asp Phe Leu 
                485 


<210>  5
<211>  1488
<212>  DNA
<213>  Saccharomyces cerevisiae

<400>  5
atgtcaaatt ctccaacagt agcaactttg tcacaagaat atttcgatcc gaatattcct       60

caaatactcc cacacgagaa aatgtataag attcaggtgg ggaaatcatt attcaaaatc      120

agcggtgctt ctttaagctc ggatggtcct agtttcttca ccgaatattt ttctaagaag      180

cgctctcctt ccaataacga tgacagtaat aacgatacca tggaatctaa taaaaatgag      240

gttctattca tagacagatc agccgaagtt tttgaatgga tctatcaaca tttgcaagga      300

tatataattg agattaagga tgaagtacaa tataccatgc ttttcgcaga tgcgatgtac      360

tacaatttac cgcgattgcg atctttactg aaagaaacgg actattattt taccaacatt      420

ggtggacaat cattcaagat agcgaaaaat ttatttagaa gagaaggaga ttccccaaat      480

tatttcgaaa tatatgcggc tacagtttat atcgatgtgg aagagctgat aatatctaaa      540

aaactattaa ggcctccatc tcattctgct ccttacattc caagatcttc tgagtacttc      600

aaagatttgc tgacgttact gggaggagca tccatcgatc tagatgataa taaaagaaat      660

gccttgataa aagagtgccg ttattacagg cttttaaatc tcgaacagcg gctaatcaaa      720

tctcatatta gctataaccc aataacaagg aaagaagaga tttgcctcct attgaaggat      780

ttgtcaaaaa aaggcatcac ttttccagct tcttctgcat tttcaacctc gccctacttt      840

gaagacgatt tctgtagcat aaatgaatgc gactcgctat caaaaacccg tgaacagcca      900

gcaaataaaa agattaaact tgacatgact gaaaaatata acgattcctg gaatatgctt      960

tgttataaga gacctttttt agataagcat ccgagagagt tgatatttca aataaattcg     1020

actgattgta cgataatcct caataaggaa agccaaagta tccatgtaga cataactgga     1080

gaatccgcct ataagtttga ggccttattt ggaagtcatt tgccaaatac accctctggt     1140

gcgcctaagt tgaaaaatta ccagtatcgg ttcccatccg atagtacgca gacgaaaatt     1200

gaaactcatt atttacttcc agcatgcata tatctctgtg atttggacat aaacggcatt     1260

aagatttctc aagttcaaac tttgctaact gataaaaata aattcaatga cagggtcatt     1320

gacgtgtctg atccacttga ccttagattt tgttctggtt taaagcttta cttaagaaag     1380

tctctctgga aactggccgt gaaagatggc aatattatgc tgatagctat taaagctata     1440

gcatttaacg gaactaagga atactacaag ggatatgaat atctgtag                  1488


<210>  6
<211>  495
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  6

Met Ser Asn Ser Pro Thr Val Ala Thr Leu Ser Gln Glu Tyr Phe Asp 
1               5                   10                  15      


Pro Asn Ile Pro Gln Ile Leu Pro His Glu Lys Met Tyr Lys Ile Gln 
            20                  25                  30          


Val Gly Lys Ser Leu Phe Lys Ile Ser Gly Ala Ser Leu Ser Ser Asp 
        35                  40                  45              


Gly Pro Ser Phe Phe Thr Glu Tyr Phe Ser Lys Lys Arg Ser Pro Ser 
    50                  55                  60                  


Asn Asn Asp Asp Ser Asn Asn Asp Thr Met Glu Ser Asn Lys Asn Glu 
65                  70                  75                  80  


Val Leu Phe Ile Asp Arg Ser Ala Glu Val Phe Glu Trp Ile Tyr Gln 
                85                  90                  95      


His Leu Gln Gly Tyr Ile Ile Glu Ile Lys Asp Glu Val Gln Tyr Thr 
            100                 105                 110         


Met Leu Phe Ala Asp Ala Met Tyr Tyr Asn Leu Pro Arg Leu Arg Ser 
        115                 120                 125             


Leu Leu Lys Glu Thr Asp Tyr Tyr Phe Thr Asn Ile Gly Gly Gln Ser 
    130                 135                 140                 


Phe Lys Ile Ala Lys Asn Leu Phe Arg Arg Glu Gly Asp Ser Pro Asn 
145                 150                 155                 160 


Tyr Phe Glu Ile Tyr Ala Ala Thr Val Tyr Ile Asp Val Glu Glu Leu 
                165                 170                 175     


Ile Ile Ser Lys Lys Leu Leu Arg Pro Pro Ser His Ser Ala Pro Tyr 
            180                 185                 190         


Ile Pro Arg Ser Ser Glu Tyr Phe Lys Asp Leu Leu Thr Leu Leu Gly 
        195                 200                 205             


Gly Ala Ser Ile Asp Leu Asp Asp Asn Lys Arg Asn Ala Leu Ile Lys 
    210                 215                 220                 


Glu Cys Arg Tyr Tyr Arg Leu Leu Asn Leu Glu Gln Arg Leu Ile Lys 
225                 230                 235                 240 


Ser His Ile Ser Tyr Asn Pro Ile Thr Arg Lys Glu Glu Ile Cys Leu 
                245                 250                 255     


Leu Leu Lys Asp Leu Ser Lys Lys Gly Ile Thr Phe Pro Ala Ser Ser 
            260                 265                 270         


Ala Phe Ser Thr Ser Pro Tyr Phe Glu Asp Asp Phe Cys Ser Ile Asn 
        275                 280                 285             


Glu Cys Asp Ser Leu Ser Lys Thr Arg Glu Gln Pro Ala Asn Lys Lys 
    290                 295                 300                 


Ile Lys Leu Asp Met Thr Glu Lys Tyr Asn Asp Ser Trp Asn Met Leu 
305                 310                 315                 320 


Cys Tyr Lys Arg Pro Phe Leu Asp Lys His Pro Arg Glu Leu Ile Phe 
                325                 330                 335     


Gln Ile Asn Ser Thr Asp Cys Thr Ile Ile Leu Asn Lys Glu Ser Gln 
            340                 345                 350         


Ser Ile His Val Asp Ile Thr Gly Glu Ser Ala Tyr Lys Phe Glu Ala 
        355                 360                 365             


Leu Phe Gly Ser His Leu Pro Asn Thr Pro Ser Gly Ala Pro Lys Leu 
    370                 375                 380                 


Lys Asn Tyr Gln Tyr Arg Phe Pro Ser Asp Ser Thr Gln Thr Lys Ile 
385                 390                 395                 400 


Glu Thr His Tyr Leu Leu Pro Ala Cys Ile Tyr Leu Cys Asp Leu Asp 
                405                 410                 415     


Ile Asn Gly Ile Lys Ile Ser Gln Val Gln Thr Leu Leu Thr Asp Lys 
            420                 425                 430         


Asn Lys Phe Asn Asp Arg Val Ile Asp Val Ser Asp Pro Leu Asp Leu 
        435                 440                 445             


Arg Phe Cys Ser Gly Leu Lys Leu Tyr Leu Arg Lys Ser Leu Trp Lys 
    450                 455                 460                 


Leu Ala Val Lys Asp Gly Asn Ile Met Leu Ile Ala Ile Lys Ala Ile 
465                 470                 475                 480 


Ala Phe Asn Gly Thr Lys Glu Tyr Tyr Lys Gly Tyr Glu Tyr Leu 
                485                 490                 495 


<210>  7
<211>  215
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  7

Met Leu Gly Ile Arg Ser Ser Val Lys Thr Cys Phe Lys Pro Met Ser 
1               5                   10                  15      


Leu Thr Ser Lys Arg Leu Ile Ser Gln Ser Leu Val Ala Ser Lys Ser 
            20                  25                  30          


Thr Tyr Arg Thr Pro Asn Phe Asp Asp Val Leu Lys Glu Asn Asn Asp 
        35                  40                  45              


Ala Asp Lys Gly Arg Ser Tyr Ala Tyr Phe Met Val Gly Ala Met Gly 
    50                  55                  60                  


Leu Leu Ser Ser Ala Gly Ala Lys Ser Thr Val Glu Thr Phe Ile Ser 
65                  70                  75                  80  


Ser Met Thr Ala Thr Ala Asp Val Leu Ala Met Ala Lys Val Glu Val 
                85                  90                  95      


Asn Leu Ala Ala Ile Pro Leu Gly Lys Asn Val Val Val Lys Trp Gln 
            100                 105                 110         


Gly Lys Pro Val Phe Ile Arg His Arg Thr Pro His Glu Ile Gln Glu 
        115                 120                 125             


Ala Asn Ser Val Asp Met Ser Ala Leu Lys Asp Pro Gln Thr Asp Ala 
    130                 135                 140                 


Asp Arg Val Lys Asp Pro Gln Trp Leu Ile Met Leu Gly Ile Cys Thr 
145                 150                 155                 160 


His Leu Gly Cys Val Pro Ile Gly Glu Ala Gly Asp Phe Gly Gly Trp 
                165                 170                 175     


Phe Cys Pro Cys His Gly Ser His Tyr Asp Ile Ser Gly Arg Ile Arg 
            180                 185                 190         


Lys Gly Pro Ala Pro Leu Asn Leu Glu Ile Pro Ala Tyr Glu Phe Asp 
        195                 200                 205             


Gly Asp Lys Val Ile Val Gly 
    210                 215 


<210>  8
<211>  92
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  8

Met Leu Gly Ile Arg Ser Ser Val Lys Thr Cys Phe Lys Pro Met Ser 
1               5                   10                  15      


Leu Thr Ser Lys Arg Leu Ile Ser Gln Ser Leu Val Ala Ser Lys Ser 
            20                  25                  30          


Thr Tyr Arg Thr Pro Asn Phe Asp Asp Val Leu Lys Glu Asn Asn Asp 
        35                  40                  45              


Ala Asp Lys Gly Arg Ser Tyr Ala Tyr Phe Met Val Gly Ala Met Gly 
    50                  55                  60                  


Leu Leu Ser Ser Ala Gly Ala Lys Ser Thr Val Glu Thr Phe Ile Ser 
65                  70                  75                  80  


Ser Met Thr Ala Thr Ala Asp Val Leu Ala Met Ala 
                85                  90          


<210>  9
<211>  125
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  9

Met Ala Lys Val Glu Val Asn Leu Ala Ala Ile Pro Leu Gly Lys Asn 
1               5                   10                  15      


Val Val Val Lys Trp Gln Gly Lys Pro Val Phe Ile Arg His Arg Thr 
            20                  25                  30          


Pro His Glu Ile Gln Glu Ala Asn Ser Val Asp Met Ser Ala Leu Lys 
        35                  40                  45              


Asp Pro Gln Thr Asp Ala Asp Arg Val Lys Asp Pro Gln Trp Leu Ile 
    50                  55                  60                  


Met Leu Gly Ile Cys Thr His Leu Gly Cys Val Pro Ile Gly Glu Ala 
65                  70                  75                  80  


Gly Asp Phe Gly Gly Trp Phe Cys Pro Cys His Gly Ser His Tyr Asp 
                85                  90                  95      


Ile Ser Gly Arg Ile Arg Lys Gly Pro Ala Pro Leu Asn Leu Glu Ile 
            100                 105                 110         


Pro Ala Tyr Glu Phe Asp Gly Asp Lys Val Ile Val Gly 
        115                 120                 125 


<210>  10
<211>  1665
<212>  DNA
<213>  Saccharomyces cerevisiae

<400>  10
atgaccgcaa agacttttct actacaggcc tccgctagtc gccctcgtag taaccatttt       60

aaaaatgagc ataataatat tccattggcg cctgtaccga tcgccccaaa taccaaccat      120

cataacaata gttcgctgga attcgaaaac gatggcagta aaaagaagaa gaagtctagc      180

ttggtggtta gaacttcaaa acattgggtt ttgcccccaa gaccaagacc tggtagaaga      240

tcatcttctc acaacactct acctgccaac aacaccaata atattttaaa tgttggccct      300

aacagcagga acagtagtaa taataataat aataataaca tcatttcgaa taggaaacaa      360

gcttccaaag aaaagaggaa aataccaaga catatccaga caatcgatga aaagctaata      420

aacgactcga attacctcgc atttttgaag ttcgatgact tggaaaatga aaagtttcat      480

tcttctgcct cctccatttc atctccatct tattcatctc catctttttc aagttataga      540

aatagaaaaa aatcagaatt catggacgat gaaagctgca ccgatgtgga aaccattgct      600

gctcacaaca gtctgctaac aaaaaaccat catatagatt cttcttcaaa tgttcacgca      660

ccacccacga aaaaatcaaa gttgaacgac tttgatttat tgtccttatc ttccacatct      720

tcatcggcca ctccggtccc acagttgaca aaagatttga acatgaacct aaattttcat      780

aagatccctc ataaggcttc attccctgat tctccagcag atttctctcc agcagattca      840

gtctcgttga ttagaaacca ctccttgcct actaatttgc aagttaagga caaaattgag      900

gatttgaacg agattaaatt ctttaacgat ttcgagaaac ttgagttttt caataagtat      960

gccaaagtca acacgaataa cgacgttaac gaaaataatg atctctggaa ttcttactta     1020

cagtctatgg acgatacaac aggtaagaac agtggcaatt accaacaagt ggacaatgac     1080

gataatatgt ctttattgaa tctgccaatt ttggaggaaa ccgtatcttc agggcaagat     1140

gataaggttg agccagatga agaagacatt tggaattatt taccaagttc aagttcacaa     1200

caagaagatt catcacgtgc tttgaaaaaa aatactaatt ctgagaaggc gaacatccaa     1260

gcaaagaacg atgaaaccta tctgtttctt caggatcagg atgaaagcgc tgattcgcat     1320

caccatgacg agttaggttc agaaatcact ttggctgaca ataagttttc ttatttgccc     1380

ccaactctag aagagttgat ggaagagcag gactgtaaca atggcagatc ttttaaaaat     1440

ttcatgtttt ccaacgatac cggtattgac ggtagtgccg gtactgatga cgactacacc     1500

aaagttctga aatccaaaaa aatttctacg tcgaagtcga acgctaacct ttatgactta     1560

aacgataaca acaatgatgc aactgccacc aatgaacttg atcaaagcag tttcatcgac     1620

gaccttgacg aagatgtcga ttttttaaag gtacaagtat tttga                     1665


<210>  11
<211>  554
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  11

Met Thr Ala Lys Thr Phe Leu Leu Gln Ala Ser Ala Ser Arg Pro Arg 
1               5                   10                  15      


Ser Asn His Phe Lys Asn Glu His Asn Asn Ile Pro Leu Ala Pro Val 
            20                  25                  30          


Pro Ile Ala Pro Asn Thr Asn His His Asn Asn Ser Ser Leu Glu Phe 
        35                  40                  45              


Glu Asn Asp Gly Ser Lys Lys Lys Lys Lys Ser Ser Leu Val Val Arg 
    50                  55                  60                  


Thr Ser Lys His Trp Val Leu Pro Pro Arg Pro Arg Pro Gly Arg Arg 
65                  70                  75                  80  


Ser Ser Ser His Asn Thr Leu Pro Ala Asn Asn Thr Asn Asn Ile Leu 
                85                  90                  95      


Asn Val Gly Pro Asn Ser Arg Asn Ser Ser Asn Asn Asn Asn Asn Asn 
            100                 105                 110         


Asn Ile Ile Ser Asn Arg Lys Gln Ala Ser Lys Glu Lys Arg Lys Ile 
        115                 120                 125             


Pro Arg His Ile Gln Thr Ile Asp Glu Lys Leu Ile Asn Asp Ser Asn 
    130                 135                 140                 


Tyr Leu Ala Phe Leu Lys Phe Asp Asp Leu Glu Asn Glu Lys Phe His 
145                 150                 155                 160 


Ser Ser Ala Ser Ser Ile Ser Ser Pro Ser Tyr Ser Ser Pro Ser Phe 
                165                 170                 175     


Ser Ser Tyr Arg Asn Arg Lys Lys Ser Glu Phe Met Asp Asp Glu Ser 
            180                 185                 190         


Cys Thr Asp Val Glu Thr Ile Ala Ala His Asn Ser Leu Leu Thr Lys 
        195                 200                 205             


Asn His His Ile Asp Ser Ser Ser Asn Val His Ala Pro Pro Thr Lys 
    210                 215                 220                 


Lys Ser Lys Leu Asn Asp Phe Asp Leu Leu Ser Leu Ser Ser Thr Ser 
225                 230                 235                 240 


Ser Ser Ala Thr Pro Val Pro Gln Leu Thr Lys Asp Leu Asn Met Asn 
                245                 250                 255     


Leu Asn Phe His Lys Ile Pro His Lys Ala Ser Phe Pro Asp Ser Pro 
            260                 265                 270         


Ala Asp Phe Ser Pro Ala Asp Ser Val Ser Leu Ile Arg Asn His Ser 
        275                 280                 285             


Leu Pro Thr Asn Leu Gln Val Lys Asp Lys Ile Glu Asp Leu Asn Glu 
    290                 295                 300                 


Ile Lys Phe Phe Asn Asp Phe Glu Lys Leu Glu Phe Phe Asn Lys Tyr 
305                 310                 315                 320 


Ala Lys Val Asn Thr Asn Asn Asp Val Asn Glu Asn Asn Asp Leu Trp 
                325                 330                 335     


Asn Ser Tyr Leu Gln Ser Met Asp Asp Thr Thr Gly Lys Asn Ser Gly 
            340                 345                 350         


Asn Tyr Gln Gln Val Asp Asn Asp Asp Asn Met Ser Leu Leu Asn Leu 
        355                 360                 365             


Pro Ile Leu Glu Glu Thr Val Ser Ser Gly Gln Asp Asp Lys Val Glu 
    370                 375                 380                 


Pro Asp Glu Glu Asp Ile Trp Asn Tyr Leu Pro Ser Ser Ser Ser Gln 
385                 390                 395                 400 


Gln Glu Asp Ser Ser Arg Ala Leu Lys Lys Asn Thr Asn Ser Glu Lys 
                405                 410                 415     


Ala Asn Ile Gln Ala Lys Asn Asp Glu Thr Tyr Leu Phe Leu Gln Asp 
            420                 425                 430         


Gln Asp Glu Ser Ala Asp Ser His His His Asp Glu Leu Gly Ser Glu 
        435                 440                 445             


Ile Thr Leu Ala Asp Asn Lys Phe Ser Tyr Leu Pro Pro Thr Leu Glu 
    450                 455                 460                 


Glu Leu Met Glu Glu Gln Asp Cys Asn Asn Gly Arg Ser Phe Lys Asn 
465                 470                 475                 480 


Phe Met Phe Ser Asn Asp Thr Gly Ile Asp Gly Ser Ala Gly Thr Asp 
                485                 490                 495     


Asp Asp Tyr Thr Lys Val Leu Lys Ser Lys Lys Ile Ser Thr Ser Lys 
            500                 505                 510         


Ser Asn Ala Asn Leu Tyr Asp Leu Asn Asp Asn Asn Asn Asp Ala Thr 
        515                 520                 525             


Ala Thr Asn Glu Leu Asp Gln Ser Ser Phe Ile Asp Asp Leu Asp Glu 
    530                 535                 540                 


Asp Val Asp Phe Leu Lys Val Gln Val Phe 
545                 550                 


<210>  12
<211>  77
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  12

Met Ala Tyr Thr Ser His Leu Ser Ser Lys Thr Gly Leu His Phe Gly 
1               5                   10                  15      


Arg Leu Ser Leu Arg Ser Leu Thr Ala Tyr Ala Pro Asn Leu Met Leu 
            20                  25                  30          


Trp Gly Gly Ala Ser Met Leu Gly Leu Phe Val Phe Thr Glu Gly Trp 
        35                  40                  45              


Pro Lys Phe Gln Asp Thr Leu Tyr Lys Lys Ile Pro Leu Leu Gly Pro 
    50                  55                  60                  


Thr Leu Glu Asp His Thr Pro Pro Glu Asp Lys Pro Asn 
65                  70                  75          


<210>  13
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  guide RNA

<400>  13
gttaacgact ccaaatgtaa                                                   20


<210>  14
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  guide RNA

<400>  14
taatgcgtcc gcgaaaagac                                                   20


<210>  15
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  guide RNA

<400>  15
ggttttatga aatccagtag                                                   20


<210>  16
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  guide RNA

<400>  16
ttcatggttt ggtaaaagct                                                   20


<210>  17
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  guide RNA

<400>  17
atattccttc gtcccagaga                                                   20


