                         SEQUENCE LISTING

<110>  Immetacyte Ltd
       
<120>  RECEPTORS PROVIDING TARGETED COSTIMULATION FOR ADOPTIVE CELL 
       	THERAPY

<130>  P37029WO1

<150>  US62/951,770
<151>  2019-12-20

<150>  GB1900858.0
<151>  2019-01-22

<160>  14    

<170>  PatentIn version 3.5

<210>  1
<211>  4900
<212>  DNA
<213>  Homo sapiens


<220>
<221>  CDS
<222>  (223)..(885)
<223>  Acession No. NM_006139

<220>
<221>  sig_peptide
<222>  (223)..(276)

<220>
<221>  transmembrane
<222>  (456)..(537)

<400>  1
taaagtcatc aaaacaacgt tatatcctgt gtgaaatgct gcagtcagga tgccttgtgg       60

tttgagtgcc ttgatcatgt gccctaaggg gatggtggcg gtggtggtgg ccgtggatga      120

cggagactct caggccttgg caggtgcgtc tttcagttcc cctcacactt cgggttcctc      180

ggggaggagg ggctggaacc ctagcccatc gtcaggacaa ag atg ctc agg ctg         234
                                               Met Leu Arg Leu            
                                               1                          

ctc ttg gct ctc aac tta ttc cct tca att caa gta aca gga aac aag        282
Leu Leu Ala Leu Asn Leu Phe Pro Ser Ile Gln Val Thr Gly Asn Lys           
5                   10                  15                  20            

att ttg gtg aag cag tcg ccc atg ctt gta gcg tac gac aat gcg gtc        330
Ile Leu Val Lys Gln Ser Pro Met Leu Val Ala Tyr Asp Asn Ala Val           
                25                  30                  35                

aac ctt agc tgc aag tat tcc tac aat ctc ttc tca agg gag ttc cgg        378
Asn Leu Ser Cys Lys Tyr Ser Tyr Asn Leu Phe Ser Arg Glu Phe Arg           
            40                  45                  50                    

gca tcc ctt cac aaa gga ctg gat agt gct gtg gaa gtc tgt gtt gta        426
Ala Ser Leu His Lys Gly Leu Asp Ser Ala Val Glu Val Cys Val Val           
        55                  60                  65                        

tat ggg aat tac tcc cag cag ctt cag gtt tac tca aaa acg ggg ttc        474
Tyr Gly Asn Tyr Ser Gln Gln Leu Gln Val Tyr Ser Lys Thr Gly Phe           
    70                  75                  80                            

aac tgt gat ggg aaa ttg ggc aat gaa tca gtg aca ttc tac ctc cag        522
Asn Cys Asp Gly Lys Leu Gly Asn Glu Ser Val Thr Phe Tyr Leu Gln           
85                  90                  95                  100           

aat ttg tat gtt aac caa aca gat att tac ttc tgc aaa att gaa gtt        570
Asn Leu Tyr Val Asn Gln Thr Asp Ile Tyr Phe Cys Lys Ile Glu Val           
                105                 110                 115               

atg tat cct cct cct tac cta gac aat gag aag agc aat gga acc att        618
Met Tyr Pro Pro Pro Tyr Leu Asp Asn Glu Lys Ser Asn Gly Thr Ile           
            120                 125                 130                   

atc cat gtg aaa ggg aaa cac ctt tgt cca agt ccc cta ttt ccc gga        666
Ile His Val Lys Gly Lys His Leu Cys Pro Ser Pro Leu Phe Pro Gly           
        135                 140                 145                       

cct tct aag ccc ttt tgg gtg ctg gtg gtg gtt ggt gga gtc ctg gct        714
Pro Ser Lys Pro Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala           
    150                 155                 160                           

tgc tat agc ttg cta gta aca gtg gcc ttt att att ttc tgg gtg agg        762
Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val Arg           
165                 170                 175                 180           

agt aag agg agc agg ctc ctg cac agt gac tac atg aac atg act ccc        810
Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr Pro           
                185                 190                 195               

cgc cgc ccc ggg ccc acc cgc aag cat tac cag ccc tat gcc cca cca        858
Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro Pro           
            200                 205                 210                   

cgc gac ttc gca gcc tat cgc tcc tga cacggacgcc tatccagaag              905
Arg Asp Phe Ala Ala Tyr Arg Ser                                           
        215                 220                                           

ccagccggct ggcagccccc atctgctcaa tatcactgct ctggatagga aatgaccgcc      965

atctccagcc ggccacctca ggcccctgtt gggccaccaa tgccaatttt tctcgagtga     1025

ctagaccaaa tatcaagatc attttgagac tctgaaatga agtaaaagag atttcctgtg     1085

acaggccaag tcttacagtg ccatggccca cattccaact taccatgtac ttagtgactt     1145

gactgagaag ttagggtaga aaacaaaaag ggagtggatt ctgggagcct cttccctttc     1205

tcactcacct gcacatctca gtcaagcaaa gtgtggtatc cacagacatt ttagttgcag     1265

aagaaaggct aggaaatcat tccttttggt taaatgggtg tttaatcttt tggttagtgg     1325

gttaaacggg gtaagttaga gtagggggag ggataggaag acatatttaa aaaccattaa     1385

aacactgtct cccactcatg aaatgagcca cgtagttcct atttaatgct gttttccttt     1445

agtttagaaa tacatagaca ttgtctttta tgaattctga tcatatttag tcattttgac     1505

caaatgaggg atttggtcaa atgagggatt ccctcaaagc aatatcaggt aaaccaagtt     1565

gctttcctca ctccctgtca tgagacttca gtgttaatgt tcacaatata ctttcgaaag     1625

aataaaatag ttctcctaca tgaagaaaga atatgtcagg aaataaggtc actttatgtc     1685

aaaattattt gagtactatg ggacctggcg cagtggctca tgcttgtaat cccagcactt     1745

tgggaggccg aggtgggcag atcacttgag atcaggacca gcctggtcaa gatggtgaaa     1805

ctccgtctgt actaaaaata caaaatttag cttggcctgg tggcaggcac ctgtaatccc     1865

agctgcccaa gaggctgagg catgagaatc gcttgaacct ggcaggcgga ggttgcagtg     1925

agccgagata gtgccacagc tctccagcct gggcgacaga gtgagactcc atctcaaaca     1985

acaacaacaa caacaacaac aacaacaaac cacaaaatta tttgagtact gtgaaggatt     2045

atttgtctaa cagttcattc caatcagacc aggtaggagc tttcctgttt catatgtttc     2105

agggttgcac agttggtctc tttaatgtcg gtgtggagat ccaaagtggg ttgtggaaag     2165

agcgtccata ggagaagtga gaatactgtg aaaaagggat gttagcattc attagagtat     2225

gaggatgagt cccaagaagg ttctttggaa ggaggacgaa tagaatggag taatgaaatt     2285

cttgccatgt gctgaggaga tagccagcat taggtgacaa tcttccagaa gtggtcaggc     2345

agaaggtgcc ctggtgagag ctcctttaca gggactttat gtggtttagg gctcagagct     2405

ccaaaactct gggctcagct gctcctgtac cttggaggtc cattcacatg ggaaagtatt     2465

ttggaatgtg tcttttgaag agagcatcag agttcttaag ggactgggta aggcctgacc     2525

ctgaaatgac catggatatt tttctaccta cagtttgagt caactagaat atgcctgggg     2585

accttgaaga atggcccttc agtggccctc accatttgtt catgcttcag ttaattcagg     2645

tgttgaagga gcttaggttt tagaggcacg tagacttggt tcaagtctcg ttagtagttg     2705

aatagcctca ggcaagtcac tgcccaccta agatgatggt tcttcaacta taaaatggag     2765

ataatggtta caaatgtctc ttcctatagt ataatctcca taagggcatg gcccaagtct     2825

gtctttgact ctgcctatcc ctgacattta gtagcatgcc cgacatacaa tgttagctat     2885

tggtattatt gccatataga taaattatgt ataaaaatta aactgggcaa tagcctaaga     2945

aggggggaat attgtaacac aaatttaaac ccactacgca gggatgaggt gctataatat     3005

gaggaccttt taacttccat cattttcctg tttcttgaaa tagtttatct tgtaatgaaa     3065

tataaggcac ctcccacttt tatgtataga aagaggtctt ttaatttttt tttaatgtga     3125

gaaggaaggg aggagtagga atcttgagat tccagatcga aaatactgta ctttggttga     3185

tttttaagtg ggcttccatt ccatggattt aatcagtccc aagaagatca aactcagcag     3245

tacttgggtg ctgaagaact gttggattta ccctggcacg tgtgccactt gccagcttct     3305

tgggcacaca gagttcttca atccaagtta tcagattgta tttgaaaatg acagagctgg     3365

agagtttttt gaaatggcag tggcaaataa ataaatactt ttttttaaat ggaaagactt     3425

gatctatggt aataaatgat tttgttttct gactggaaaa ataggcctac taaagatgaa     3485

tcacacttga gatgtttctt actcactctg cacagaaaca aagaagaaat gttatacagg     3545

gaagtccgtt ttcactatta gtatgaacca agaaatggtt caaaaacagt ggtaggagca     3605

atgctttcat agtttcagat atggtagtta tgaagaaaac aatgtcattt gctgctatta     3665

ttgtaagagt cttataatta atggtactcc tataattttt gattgtgagc tcacctattt     3725

gggttaagca tgccaattta aagagaccaa gtgtatgtac attatgttct acatattcag     3785

tgataaaatt actaaactac tatatgtctg ctttaaattt gtactttaat attgtctttt     3845

ggtattaaga aagatatgct ttcagaatag atatgcttcg ctttggcaag gaatttggat     3905

agaacttgct atttaaaaga ggtgtggggt aaatccttgt ataaatctcc agtttagcct     3965

tttttgaaaa agctagactt tcaaatacta atttcacttc aagcagggta cgtttctggt     4025

ttgtttgctt gacttcagtc acaatttctt atcagaccaa tggctgacct ctttgagatg     4085

tcaggctagg cttacctatg tgttctgtgt catgtgaatg ctgagaagtt tgacagagat     4145

ccaacttcag ccttgacccc atcagtccct cgggttaact aactgagcca ccggtcctca     4205

tggctatttt aatgagggta ttgatggtta aatgcatgtc tgatccctta tcccagccat     4265

ttgcactgcc agctgggaac tataccagac ctggatactg atcccaaagt gttaaattca     4325

actacatgct ggagattaga gatggtgcca ataaaggacc cagaaccagg atcttgattg     4385

ctatagactt attaataatc caggtcaaag agagtgacac acactctctc aagacctggg     4445

gtgagggagt ctgtgttatc tgcaaggcca tttgaggctc agaaagtctc tctttcctat     4505

agatatatgc atactttctg acatatagga atgtatcagg aatactcaac catcacaggc     4565

atgttcctac ctcagggcct ttacatgtcc tgtttactct gtctagaatg tccttctgta     4625

gatgacctgg cttgcctcgt cacccttcag gtccttgctc aagtgtcatc ttctccccta     4685

gttaaactac cccacaccct gtctgctttc cttgcttatt tttctccata gcattttacc     4745

atctcttaca ttagacattt ttcttattta tttgtagttt ataagcttca tgaggcaagt     4805

aactttgctt tgtttcttgc tgtatctcca gtgcccagag cagtgcctgg tatataataa     4865

atatttattg actgagtgaa aaaaaaaaaa aaaaa                                4900


<210>  2
<211>  220
<212>  PRT
<213>  Homo sapiens

<400>  2

Met Leu Arg Leu Leu Leu Ala Leu Asn Leu Phe Pro Ser Ile Gln Val 
1               5                   10                  15      


Thr Gly Asn Lys Ile Leu Val Lys Gln Ser Pro Met Leu Val Ala Tyr 
            20                  25                  30          


Asp Asn Ala Val Asn Leu Ser Cys Lys Tyr Ser Tyr Asn Leu Phe Ser 
        35                  40                  45              


Arg Glu Phe Arg Ala Ser Leu His Lys Gly Leu Asp Ser Ala Val Glu 
    50                  55                  60                  


Val Cys Val Val Tyr Gly Asn Tyr Ser Gln Gln Leu Gln Val Tyr Ser 
65                  70                  75                  80  


Lys Thr Gly Phe Asn Cys Asp Gly Lys Leu Gly Asn Glu Ser Val Thr 
                85                  90                  95      


Phe Tyr Leu Gln Asn Leu Tyr Val Asn Gln Thr Asp Ile Tyr Phe Cys 
            100                 105                 110         


Lys Ile Glu Val Met Tyr Pro Pro Pro Tyr Leu Asp Asn Glu Lys Ser 
        115                 120                 125             


Asn Gly Thr Ile Ile His Val Lys Gly Lys His Leu Cys Pro Ser Pro 
    130                 135                 140                 


Leu Phe Pro Gly Pro Ser Lys Pro Phe Trp Val Leu Val Val Val Gly 
145                 150                 155                 160 


Gly Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile 
                165                 170                 175     


Phe Trp Val Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met 
            180                 185                 190         


Asn Met Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro 
        195                 200                 205             


Tyr Ala Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser 
    210                 215                 220 


<210>  3
<211>  165
<212>  PRT
<213>  Homo sapiens

<400>  3

Asn Lys Ile Leu Val Lys Gln Ser Pro Met Leu Val Ala Tyr Asp Asn 
1               5                   10                  15      


Ala Val Asn Leu Ser Cys Lys Tyr Ser Tyr Asn Leu Phe Ser Arg Glu 
            20                  25                  30          


Phe Arg Ala Ser Leu His Lys Gly Leu Asp Ser Ala Val Glu Val Cys 
        35                  40                  45              


Val Val Tyr Gly Asn Tyr Ser Gln Gln Leu Gln Val Tyr Ser Lys Thr 
    50                  55                  60                  


Gly Phe Asn Cys Asp Gly Lys Leu Gly Asn Glu Ser Val Thr Phe Tyr 
65                  70                  75                  80  


Leu Gln Asn Leu Tyr Val Asn Gln Thr Asp Ile Tyr Phe Cys Lys Ile 
                85                  90                  95      


Glu Val Met Tyr Pro Pro Pro Tyr Leu Asp Asn Glu Lys Ser Asn Gly 
            100                 105                 110         


Thr Ile Ile His Val Lys Gly Lys His Leu Cys Pro Ser Pro Leu Phe 
        115                 120                 125             


Pro Gly Pro Ser Lys Pro Phe Trp Val Leu Val Val Val Gly Gly Val 
    130                 135                 140                 


Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp 
145                 150                 155                 160 


Val Arg Ser Lys Arg 
                165 


<210>  4
<211>  248
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Fusion protein


<220>
<221>  DOMAIN
<222>  (1)..(166)
<223>  CD28

<220>
<221>  DOMAIN
<222>  (167)..(248)
<223>  CD137

<400>  4

Asn Lys Ile Leu Val Lys Gln Ser Pro Met Leu Val Ala Tyr Asp Asn 
1               5                   10                  15      


Ala Val Asn Leu Ser Cys Lys Tyr Ser Tyr Asn Leu Phe Ser Arg Glu 
            20                  25                  30          


Phe Arg Ala Ser Leu His Lys Gly Leu Asp Ser Ala Val Glu Val Cys 
        35                  40                  45              


Val Val Tyr Gly Asn Tyr Ser Gln Gln Leu Gln Val Tyr Ser Lys Thr 
    50                  55                  60                  


Gly Phe Asn Cys Asp Gly Lys Leu Gly Asn Glu Ser Val Thr Phe Tyr 
65                  70                  75                  80  


Leu Gln Asn Leu Tyr Val Asn Gln Thr Asp Ile Tyr Phe Cys Lys Ile 
                85                  90                  95      


Glu Val Met Tyr Pro Pro Pro Tyr Leu Asp Asn Glu Lys Ser Asn Gly 
            100                 105                 110         


Thr Ile Ile His Val Lys Gly Lys His Leu Cys Pro Ser Pro Leu Phe 
        115                 120                 125             


Pro Gly Pro Ser Lys Pro Phe Trp Val Leu Val Val Val Gly Gly Val 
    130                 135                 140                 


Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp 
145                 150                 155                 160 


Val Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met 
                165                 170                 175     


Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala 
            180                 185                 190         


Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser Arg Phe Ser Val Val Lys 
        195                 200                 205             


Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg 
    210                 215                 220                 


Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro 
225                 230                 235                 240 


Glu Glu Glu Glu Gly Gly Cys Glu 
                245             


<210>  5
<211>  239
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Fusion protein


<220>
<221>  DOMAIN
<222>  (1)..(202)
<223>  CD28

<220>
<221>  DOMAIN
<222>  (203)..(239)
<223>  CD134

<400>  5

Asn Lys Ile Leu Val Lys Gln Ser Pro Met Leu Val Ala Tyr Asp Asn 
1               5                   10                  15      


Ala Val Asn Leu Ser Cys Lys Tyr Ser Tyr Asn Leu Phe Ser Arg Glu 
            20                  25                  30          


Phe Arg Ala Ser Leu His Lys Gly Leu Asp Ser Ala Val Glu Val Cys 
        35                  40                  45              


Val Val Tyr Gly Asn Tyr Ser Gln Gln Leu Gln Val Tyr Ser Lys Thr 
    50                  55                  60                  


Gly Phe Asn Cys Asp Gly Lys Leu Gly Asn Glu Ser Val Thr Phe Tyr 
65                  70                  75                  80  


Leu Gln Asn Leu Tyr Val Asn Gln Thr Asp Ile Tyr Phe Cys Lys Ile 
                85                  90                  95      


Glu Val Met Tyr Pro Pro Pro Tyr Leu Asp Asn Glu Lys Ser Asn Gly 
            100                 105                 110         


Thr Ile Ile His Val Lys Gly Lys His Leu Cys Pro Ser Pro Leu Phe 
        115                 120                 125             


Pro Gly Pro Ser Lys Pro Phe Trp Val Leu Val Val Val Gly Gly Val 
    130                 135                 140                 


Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp 
145                 150                 155                 160 


Val Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met 
                165                 170                 175     


Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala 
            180                 185                 190         


Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser Arg Arg Asp Gln Arg Leu 
        195                 200                 205             


Pro Pro Asp Ala His Lys Pro Pro Gly Gly Gly Ser Phe Arg Thr Pro 
    210                 215                 220                 


Ile Gln Glu Glu Gln Ala Asp Ala His Ser Thr Leu Ala Lys Ile 
225                 230                 235                 


<210>  6
<211>  318
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Fusion protein


<220>
<221>  DOMAIN
<222>  (1)..(202)
<223>  CD28

<220>
<221>  DOMAIN
<222>  (203)..(318)
<223>  CD2

<400>  6

Asn Lys Ile Leu Val Lys Gln Ser Pro Met Leu Val Ala Tyr Asp Asn 
1               5                   10                  15      


Ala Val Asn Leu Ser Cys Lys Tyr Ser Tyr Asn Leu Phe Ser Arg Glu 
            20                  25                  30          


Phe Arg Ala Ser Leu His Lys Gly Leu Asp Ser Ala Val Glu Val Cys 
        35                  40                  45              


Val Val Tyr Gly Asn Tyr Ser Gln Gln Leu Gln Val Tyr Ser Lys Thr 
    50                  55                  60                  


Gly Phe Asn Cys Asp Gly Lys Leu Gly Asn Glu Ser Val Thr Phe Tyr 
65                  70                  75                  80  


Leu Gln Asn Leu Tyr Val Asn Gln Thr Asp Ile Tyr Phe Cys Lys Ile 
                85                  90                  95      


Glu Val Met Tyr Pro Pro Pro Tyr Leu Asp Asn Glu Lys Ser Asn Gly 
            100                 105                 110         


Thr Ile Ile His Val Lys Gly Lys His Leu Cys Pro Ser Pro Leu Phe 
        115                 120                 125             


Pro Gly Pro Ser Lys Pro Phe Trp Val Leu Val Val Val Gly Gly Val 
    130                 135                 140                 


Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp 
145                 150                 155                 160 


Val Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met 
                165                 170                 175     


Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala 
            180                 185                 190         


Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser Lys Arg Lys Lys Gln Arg 
        195                 200                 205             


Ser Arg Arg Asn Asp Glu Glu Leu Glu Thr Arg Ala His Arg Val Ala 
    210                 215                 220                 


Thr Glu Glu Arg Gly Arg Lys Pro His Gln Ile Pro Ala Ser Thr Pro 
225                 230                 235                 240 


Gln Asn Pro Ala Thr Ser Gln His Pro Pro Pro Pro Pro Gly His Arg 
                245                 250                 255     


Ser Gln Ala Pro Ser His Arg Pro Pro Pro Pro Gly His Arg Val Gln 
            260                 265                 270         


His Gln Pro Gln Lys Arg Pro Pro Ala Pro Ser Gly Thr Gln Val His 
        275                 280                 285             


Gln Gln Lys Gly Pro Pro Leu Pro Arg Pro Arg Val Gln Pro Lys Pro 
    290                 295                 300                 


Pro His Gly Ala Ala Glu Asn Ser Leu Ser Pro Ser Ser Asn 
305                 310                 315             


<210>  7
<211>  249
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Fusion protein


<220>
<221>  DOMAIN
<222>  (1)..(202)
<223>  CD28

<220>
<221>  DOMAIN
<222>  (202)..(249)
<223>  CD29

<400>  7

Asn Lys Ile Leu Val Lys Gln Ser Pro Met Leu Val Ala Tyr Asp Asn 
1               5                   10                  15      


Ala Val Asn Leu Ser Cys Lys Tyr Ser Tyr Asn Leu Phe Ser Arg Glu 
            20                  25                  30          


Phe Arg Ala Ser Leu His Lys Gly Leu Asp Ser Ala Val Glu Val Cys 
        35                  40                  45              


Val Val Tyr Gly Asn Tyr Ser Gln Gln Leu Gln Val Tyr Ser Lys Thr 
    50                  55                  60                  


Gly Phe Asn Cys Asp Gly Lys Leu Gly Asn Glu Ser Val Thr Phe Tyr 
65                  70                  75                  80  


Leu Gln Asn Leu Tyr Val Asn Gln Thr Asp Ile Tyr Phe Cys Lys Ile 
                85                  90                  95      


Glu Val Met Tyr Pro Pro Pro Tyr Leu Asp Asn Glu Lys Ser Asn Gly 
            100                 105                 110         


Thr Ile Ile His Val Lys Gly Lys His Leu Cys Pro Ser Pro Leu Phe 
        115                 120                 125             


Pro Gly Pro Ser Lys Pro Phe Trp Val Leu Val Val Val Gly Gly Val 
    130                 135                 140                 


Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp 
145                 150                 155                 160 


Val Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met 
                165                 170                 175     


Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala 
            180                 185                 190         


Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser Lys Leu Leu Met Ile Ile 
        195                 200                 205             


His Asp Arg Arg Glu Phe Ala Lys Phe Glu Lys Glu Lys Met Asn Ala 
    210                 215                 220                 


Lys Trp Asp Thr Gly Glu Asn Pro Ile Tyr Lys Ser Ala Val Thr Thr 
225                 230                 235                 240 


Val Val Asn Pro Lys Tyr Glu Gly Lys 
                245                 


<210>  8
<211>  260
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Fusion protein


<220>
<221>  DOMAIN
<222>  (1)..(202)
<223>  CD28

<220>
<221>  DOMAIN
<222>  (203)..(260)
<223>  GITR

<400>  8

Asn Lys Ile Leu Val Lys Gln Ser Pro Met Leu Val Ala Tyr Asp Asn 
1               5                   10                  15      


Ala Val Asn Leu Ser Cys Lys Tyr Ser Tyr Asn Leu Phe Ser Arg Glu 
            20                  25                  30          


Phe Arg Ala Ser Leu His Lys Gly Leu Asp Ser Ala Val Glu Val Cys 
        35                  40                  45              


Val Val Tyr Gly Asn Tyr Ser Gln Gln Leu Gln Val Tyr Ser Lys Thr 
    50                  55                  60                  


Gly Phe Asn Cys Asp Gly Lys Leu Gly Asn Glu Ser Val Thr Phe Tyr 
65                  70                  75                  80  


Leu Gln Asn Leu Tyr Val Asn Gln Thr Asp Ile Tyr Phe Cys Lys Ile 
                85                  90                  95      


Glu Val Met Tyr Pro Pro Pro Tyr Leu Asp Asn Glu Lys Ser Asn Gly 
            100                 105                 110         


Thr Ile Ile His Val Lys Gly Lys His Leu Cys Pro Ser Pro Leu Phe 
        115                 120                 125             


Pro Gly Pro Ser Lys Pro Phe Trp Val Leu Val Val Val Gly Gly Val 
    130                 135                 140                 


Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp 
145                 150                 155                 160 


Val Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met 
                165                 170                 175     


Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala 
            180                 185                 190         


Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser Gln Leu Gly Leu His Ile 
        195                 200                 205             


Trp Gln Leu Arg Ser Gln Cys Met Trp Pro Arg Glu Thr Gln Leu Leu 
    210                 215                 220                 


Leu Glu Val Pro Pro Ser Thr Glu Asp Ala Arg Ser Cys Gln Phe Pro 
225                 230                 235                 240 


Glu Glu Glu Arg Gly Glu Arg Ser Ala Glu Glu Lys Gly Arg Leu Gly 
                245                 250                 255     


Asp Leu Trp Val 
            260 


<210>  9
<211>  288
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Fusion protein


<220>
<221>  DOMAIN
<222>  (1)..(202)
<223>  CD28

<220>
<221>  DOMAIN
<222>  (203)..(288)
<223>  IL2Rg

<400>  9

Asn Lys Ile Leu Val Lys Gln Ser Pro Met Leu Val Ala Tyr Asp Asn 
1               5                   10                  15      


Ala Val Asn Leu Ser Cys Lys Tyr Ser Tyr Asn Leu Phe Ser Arg Glu 
            20                  25                  30          


Phe Arg Ala Ser Leu His Lys Gly Leu Asp Ser Ala Val Glu Val Cys 
        35                  40                  45              


Val Val Tyr Gly Asn Tyr Ser Gln Gln Leu Gln Val Tyr Ser Lys Thr 
    50                  55                  60                  


Gly Phe Asn Cys Asp Gly Lys Leu Gly Asn Glu Ser Val Thr Phe Tyr 
65                  70                  75                  80  


Leu Gln Asn Leu Tyr Val Asn Gln Thr Asp Ile Tyr Phe Cys Lys Ile 
                85                  90                  95      


Glu Val Met Tyr Pro Pro Pro Tyr Leu Asp Asn Glu Lys Ser Asn Gly 
            100                 105                 110         


Thr Ile Ile His Val Lys Gly Lys His Leu Cys Pro Ser Pro Leu Phe 
        115                 120                 125             


Pro Gly Pro Ser Lys Pro Phe Trp Val Leu Val Val Val Gly Gly Val 
    130                 135                 140                 


Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp 
145                 150                 155                 160 


Val Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met 
                165                 170                 175     


Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala 
            180                 185                 190         


Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser Glu Arg Thr Met Pro Arg 
        195                 200                 205             


Ile Pro Thr Leu Lys Asn Leu Glu Asp Leu Val Thr Glu Tyr His Gly 
    210                 215                 220                 


Asn Phe Ser Ala Trp Ser Gly Val Ser Lys Gly Leu Ala Glu Ser Leu 
225                 230                 235                 240 


Gln Pro Asp Tyr Ser Glu Arg Leu Cys Leu Val Ser Glu Ile Pro Pro 
                245                 250                 255     


Lys Gly Gly Ala Leu Gly Glu Gly Pro Gly Ala Ser Pro Cys Asn Gln 
            260                 265                 270         


His Ser Pro Tyr Trp Ala Pro Pro Cys Tyr Thr Leu Lys Pro Glu Thr 
        275                 280                 285             


<210>  10
<211>  264
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Fusion protein


<220>
<221>  DOMAIN
<222>  (1)..(202)
<223>  CD28

<220>
<221>  DOMAIN
<222>  (203)..(264)
<223>  CD40

<400>  10

Asn Lys Ile Leu Val Lys Gln Ser Pro Met Leu Val Ala Tyr Asp Asn 
1               5                   10                  15      


Ala Val Asn Leu Ser Cys Lys Tyr Ser Tyr Asn Leu Phe Ser Arg Glu 
            20                  25                  30          


Phe Arg Ala Ser Leu His Lys Gly Leu Asp Ser Ala Val Glu Val Cys 
        35                  40                  45              


Val Val Tyr Gly Asn Tyr Ser Gln Gln Leu Gln Val Tyr Ser Lys Thr 
    50                  55                  60                  


Gly Phe Asn Cys Asp Gly Lys Leu Gly Asn Glu Ser Val Thr Phe Tyr 
65                  70                  75                  80  


Leu Gln Asn Leu Tyr Val Asn Gln Thr Asp Ile Tyr Phe Cys Lys Ile 
                85                  90                  95      


Glu Val Met Tyr Pro Pro Pro Tyr Leu Asp Asn Glu Lys Ser Asn Gly 
            100                 105                 110         


Thr Ile Ile His Val Lys Gly Lys His Leu Cys Pro Ser Pro Leu Phe 
        115                 120                 125             


Pro Gly Pro Ser Lys Pro Phe Trp Val Leu Val Val Val Gly Gly Val 
    130                 135                 140                 


Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp 
145                 150                 155                 160 


Val Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met 
                165                 170                 175     


Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala 
            180                 185                 190         


Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser Lys Lys Val Ala Lys Lys 
        195                 200                 205             


Pro Thr Asn Lys Ala Pro His Pro Lys Gln Glu Pro Gln Glu Ile Asn 
    210                 215                 220                 


Phe Pro Asp Asp Leu Pro Gly Ser Asn Thr Ala Ala Pro Val Gln Glu 
225                 230                 235                 240 


Thr Leu His Gly Cys Gln Pro Val Thr Gln Glu Asp Gly Lys Glu Ser 
                245                 250                 255     


Arg Ile Ser Val Gln Glu Arg Gln 
            260                 


<210>  11
<211>  277
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Protein fusion


<220>
<221>  DOMAIN
<222>  (1)..(202)
<223>  CD28

<220>
<221>  DOMAIN
<222>  (203)..(277)
<223>  CD150

<400>  11

Asn Lys Ile Leu Val Lys Gln Ser Pro Met Leu Val Ala Tyr Asp Asn 
1               5                   10                  15      


Ala Val Asn Leu Ser Cys Lys Tyr Ser Tyr Asn Leu Phe Ser Arg Glu 
            20                  25                  30          


Phe Arg Ala Ser Leu His Lys Gly Leu Asp Ser Ala Val Glu Val Cys 
        35                  40                  45              


Val Val Tyr Gly Asn Tyr Ser Gln Gln Leu Gln Val Tyr Ser Lys Thr 
    50                  55                  60                  


Gly Phe Asn Cys Asp Gly Lys Leu Gly Asn Glu Ser Val Thr Phe Tyr 
65                  70                  75                  80  


Leu Gln Asn Leu Tyr Val Asn Gln Thr Asp Ile Tyr Phe Cys Lys Ile 
                85                  90                  95      


Glu Val Met Tyr Pro Pro Pro Tyr Leu Asp Asn Glu Lys Ser Asn Gly 
            100                 105                 110         


Thr Ile Ile His Val Lys Gly Lys His Leu Cys Pro Ser Pro Leu Phe 
        115                 120                 125             


Pro Gly Pro Ser Lys Pro Phe Trp Val Leu Val Val Val Gly Gly Val 
    130                 135                 140                 


Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp 
145                 150                 155                 160 


Val Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met 
                165                 170                 175     


Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala 
            180                 185                 190         


Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser Arg Arg Arg Gly Lys Thr 
        195                 200                 205             


Asn His Tyr Gln Thr Thr Val Glu Lys Lys Ser Leu Thr Ile Tyr Ala 
    210                 215                 220                 


Gln Val Gln Lys Pro Gly Pro Leu Gln Lys Lys Leu Asp Ser Phe Pro 
225                 230                 235                 240 


Ala Gln Asp Pro Cys Thr Thr Ile Tyr Val Ala Ala Thr Glu Pro Val 
                245                 250                 255     


Pro Glu Ser Val Gln Glu Thr Asn Ser Ile Thr Val Tyr Ala Ser Val 
            260                 265                 270         


Thr Leu Pro Glu Ser 
        275         


<210>  12
<211>  380
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Protein fusion


<220>
<221>  DOMAIN
<222>  (1)..(202)
<223>  CD28

<220>
<221>  DOMAIN
<222>  (203)..(318)
<223>  CD2

<220>
<221>  DOMAIN
<222>  (319)..(350)
<223>  CD40

<220>
<221>  DOMAIN
<222>  (319)..(380)
<223>  CD40

<400>  12

Asn Lys Ile Leu Val Lys Gln Ser Pro Met Leu Val Ala Tyr Asp Asn 
1               5                   10                  15      


Ala Val Asn Leu Ser Cys Lys Tyr Ser Tyr Asn Leu Phe Ser Arg Glu 
            20                  25                  30          


Phe Arg Ala Ser Leu His Lys Gly Leu Asp Ser Ala Val Glu Val Cys 
        35                  40                  45              


Val Val Tyr Gly Asn Tyr Ser Gln Gln Leu Gln Val Tyr Ser Lys Thr 
    50                  55                  60                  


Gly Phe Asn Cys Asp Gly Lys Leu Gly Asn Glu Ser Val Thr Phe Tyr 
65                  70                  75                  80  


Leu Gln Asn Leu Tyr Val Asn Gln Thr Asp Ile Tyr Phe Cys Lys Ile 
                85                  90                  95      


Glu Val Met Tyr Pro Pro Pro Tyr Leu Asp Asn Glu Lys Ser Asn Gly 
            100                 105                 110         


Thr Ile Ile His Val Lys Gly Lys His Leu Cys Pro Ser Pro Leu Phe 
        115                 120                 125             


Pro Gly Pro Ser Lys Pro Phe Trp Val Leu Val Val Val Gly Gly Val 
    130                 135                 140                 


Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp 
145                 150                 155                 160 


Val Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met 
                165                 170                 175     


Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala 
            180                 185                 190         


Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser Lys Arg Lys Lys Gln Arg 
        195                 200                 205             


Ser Arg Arg Asn Asp Glu Glu Leu Glu Thr Arg Ala His Arg Val Ala 
    210                 215                 220                 


Thr Glu Glu Arg Gly Arg Lys Pro His Gln Ile Pro Ala Ser Thr Pro 
225                 230                 235                 240 


Gln Asn Pro Ala Thr Ser Gln His Pro Pro Pro Pro Pro Gly His Arg 
                245                 250                 255     


Ser Gln Ala Pro Ser His Arg Pro Pro Pro Pro Gly His Arg Val Gln 
            260                 265                 270         


His Gln Pro Gln Lys Arg Pro Pro Ala Pro Ser Gly Thr Gln Val His 
        275                 280                 285             


Gln Gln Lys Gly Pro Pro Leu Pro Arg Pro Arg Val Gln Pro Lys Pro 
    290                 295                 300                 


Pro His Gly Ala Ala Glu Asn Ser Leu Ser Pro Ser Ser Asn Lys Lys 
305                 310                 315                 320 


Val Ala Lys Lys Pro Thr Asn Lys Ala Pro His Pro Lys Gln Glu Pro 
                325                 330                 335     


Gln Glu Ile Asn Phe Pro Asp Asp Leu Pro Gly Ser Asn Thr Ala Ala 
            340                 345                 350         


Pro Val Gln Glu Thr Leu His Gly Cys Gln Pro Val Thr Gln Glu Asp 
        355                 360                 365             


Gly Lys Glu Ser Arg Ile Ser Val Gln Glu Arg Gln 
    370                 375                 380 


<210>  13
<211>  98
<212>  PRT
<213>  Homo sapiens


<220>
<221>  DOMAIN
<222>  (1)..(98)
<223>  CD28 Extracellular truncation

<400>  13

Asn Lys Ile Leu Val Lys Gln Ser Pro Met Leu Val Ala Tyr Asp Asn 
1               5                   10                  15      


Ala Val Asn Leu Ser Cys Lys Tyr Ser Tyr Asn Leu Phe Ser Arg Glu 
            20                  25                  30          


Phe Arg Ala Ser Leu His Lys Gly Leu Asp Ser Ala Val Glu Val Cys 
        35                  40                  45              


Val Val Tyr Gly Asn Tyr Ser Gln Gln Leu Gln Val Tyr Ser Lys Thr 
    50                  55                  60                  


Gly Phe Asn Cys Asp Gly Lys Leu Gly Asn Glu Ser Val Thr Phe Tyr 
65                  70                  75                  80  


Leu Gln Asn Leu Tyr Val Asn Gln Thr Asp Ile Tyr Phe Cys Lys Ile 
                85                  90                  95      


Glu Val 
        

<210>  14
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Protein Tag

<400>  14

Asp Tyr Lys Asp Asp Asp Asp His 
1               5                   