                         SEQUENCE LISTING

<110>  Universitaet zu Luebeck
 
<120>  Antisense drug against the human intercellular adhesion molecule 
       1 (ICAM-1)

<130>  L 2418WO

<150>  EP 19 153 666.3
<151>  2019-01-25

<160>  16    

<170>  PatentIn version 3.5

<210>  1
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Gapmer oliguncleotide with a central single stranded DNA 
       oligonucleotide sequence flanked on both ends by single stranded 
       2'-O-methyl RNA oligonucleotide sequences and having 
       phosphorothioate-modified internucleotide phosphates


<220>
<221>  misc_feature
<222>  (1)..(4)
<223>  2'-O-methyladenosine, um, 2'-O-methyl-5-methylcytidine, 
       2'-O-methyladenosine

<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  phosphorothioate-modified internucleotide phosphates

<220>
<221>  misc_feature
<222>  (9)..(9)
<223>  2'-deoxy-5-methylcytidine

<220>
<221>  misc_feature
<222>  (14)..(15)
<223>  2'-deoxy-5-methylcytidine, 2'-deoxy-5-methylcytidine

<220>
<221>  misc_feature
<222>  (17)..(20)
<223>  2'-O-methyladenosine, gm, um, gm

<400>  1
ancagatgcg tggcctannn                                                   20


<210>  2
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  central oligonucleotide sequence with monophosphorothioate 
       internucleotide linkages flanked on both ends by locked nucleic 
       acids


<220>
<221>  misc_feature
<222>  (1)..(4)
<223>  locked nucleic acid modified nucleotides

<220>
<221>  misc_feature
<222>  (4)..(16)
<223>  phosphorothioate-modified internucleotide phosphates between 
       nucleotides 4 to 16

<220>
<221>  misc_feature
<222>  (17)..(20)
<223>  locked nucleic acid modified nucleotides

<400>  2
atcagatgcg tggcctagtg                                                   20


<210>  3
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide with 2'-fluoro-modified riboses


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  2'-fluoro-modified riboses

<400>  3
atcagatgcg tggcctagtg                                                   20


<210>  4
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Gapmer oliguncleotide with a central single stranded 
       oligonucleotide sequence flanked on both ends by single stranded 
       2'-fluoro-modified oligonucleotide sequences and having 
       phosphorothioate-modified internucleotide phosphates


<220>
<221>  misc_feature
<222>  (1)..(4)
<223>  2'-fluoro-modified riboses

<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  phosphorothioate-modified internucleotide phosphates

<220>
<221>  misc_feature
<222>  (17)..(20)
<223>  2'-fluoro-modified riboses

<400>  4
atcagatgcg tggcctagtg                                                   20


<210>  5
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide with 2'-O-methyl-modified riboses


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  2'-O-methyl-modified riboses

<400>  5
atcagatgcg tggcctagtg                                                   20


<210>  6
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide having 2'-O-methyl-modified riboses and having 
       phosphorothioate-modified internucleotide phosphates


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  2'-O-methyl-modified riboses

<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  phosphorothioate-modified internucleotide phosphates

<400>  6
atcagatgcg tggcctagtg                                                   20


<210>  7
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Gapmer oliguncleotide with a central single stranded 
       oligonucleotide sequence flanked on both ends by single stranded 
       2'-O-methyl-modified oligonucleotide sequences and having 
       phosphorothioate-modified internucleotide phosphates


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  phosphorothioate-modified internucleotide phosphates

<220>
<221>  misc_feature
<222>  (1)..(4)
<223>  2'-O-methyl-modified riboses

<220>
<221>  misc_feature
<222>  (17)..(20)
<223>  2'-O-methyl-modified riboses

<400>  7
atcagatgcg tggcctagtg                                                   20


<210>  8
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide having phosphorothioate-modified internucleotide 
       phosphates


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  phosphorothioate-modified internucleotide phosphates

<400>  8
atcagatgcg tggcctagtg                                                   20


<210>  9
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Gapmer oliguncleotide with a central single stranded DNA 
       oligonucleotide sequence flanked on both ends by single stranded 
       2'-O-methyl RNA oligonucleotide sequences and having 
       phosphorothioate-modified internucleotide phosphates


<220>
<221>  misc_feature
<222>  (1)..(4)
<223>  2'-O-methyladenosine, um, cm, 2'-O-methyladenosine

<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  phosphorothioate-modified internucleotide phosphates

<220>
<221>  misc_feature
<222>  (17)..(20)
<223>  2'-O-methyladenosine, gm, um, gm

<400>  9
annagatgcg tggcctannn                                                   20


<210>  10
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Gapmer oliguncleotide with a central single stranded 
       oligonucleotide sequence flanked on both ends by single stranded 
       2'-O-methyl-modified oligonucleotide sequences and having 
       phosphorothioate-modified internucleotide phosphates


<220>
<221>  misc_feature
<222>  (1)..(4)
<223>  2'-O-methyladenosine, tm, cm, 2'-O-methyladenosine

<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  phosphorothioate-modified internucleotide phosphates

<220>
<221>  misc_feature
<222>  (17)..(20)
<223>  2'-O-methyladenosine, gm, tm, gm

<400>  10
annagattcg gggcctannn                                                   20


<210>  11
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  unmodified DNA

<400>  11
atcagatgcg tggcctagtg                                                   20


<210>  12
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide having phosphorothioate-modified internucleotide 
       phosphates


<220>
<221>  misc_feature
<222>  (1)..(18)
<223>  phosphorothioate-modified internucleotide phosphates

<400>  12
ggtcagacca gtgagttc                                                     18


<210>  13
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward Primer for ICAM-1 cDNA

<400>  13
gccacttctt ctgtaagtct gtggg                                             25


<210>  14
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse Primer for ICAM-1 cDNA

<400>  14
ctaccggccc tgggacg                                                      17


<210>  15
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward Primer for cDNA encoding human GAPDH

<400>  15
aacagcgaca cccactcctc                                                   20


<210>  16
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse Primer for cDNA encoding human GAPDH

<400>  16
ggaggggaga ttcagtgtgg t                                                 21


