                         SEQUENCE LISTING

<110>  Camena Bioscience Limited
       Fraser, Andrew G
       Stemple, Derek L
       Mankowska, Sylwia A
 
<120>  COMPOSITIONS AND METHODS FOR TEMPLATE-FREE GEOMETRIC ENZYMATIC 
       NUCLEIC ACID SYNTHESIS

<130>  DNWR-006/001WO

<150>  US 62/671,091
<151>  2019-01-14

<160>  81    

<170>  PatentIn version 3.5

<210>  1
<211>  168
<212>  DNA
<213>  Aequorea victoria

<400>  1
tacacacgaa taaaagataa caaagatgag taaaggagaa gaacttttca ctggagttgt       60

cccaattctt gttgaattag atggcgatgt taatgggcaa aaattctctg tcagtggaga      120

gggtgaaggt gatgcaacat acggaaaact tacccttaaa tttatttg                   168


<210>  2
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  N-mer

<400>  2
tacacacgaa taaaagataa c                                                 21


<210>  3
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  N-mer

<400>  3
acttttcact ggagttgtcc c                                                 21


<210>  4
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  N-mer

<400>  4
cgatgttaat gggcaaaaat t                                                 21


<210>  5
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  N-mer

<400>  5
aggtgatgca acatacggaa a                                                 21


<210>  6
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  N-mer

<400>  6
aaagatgagt aaaggagaag a                                                 21


<210>  7
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  N-mer

<400>  7
aattcttgtt gaattagatg g                                                 21


<210>  8
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  N-mer

<400>  8
ctctgtcagt ggagagggtg a                                                 21


<210>  9
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  N-mer

<400>  9
acttaccctt aaatttattt g                                                 21


<210>  10
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Anchor primer

<400>  10
gatatcgagt cttaag                                                       16


<210>  11
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Short reverse complementary oligonucleotide

<400>  11
ctatagctca gaattc                                                       16


<210>  12
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Anchor primers


<220>
<221>  misc_feature
<222>  (23)..(23)
<223>  fluorescein-labeled nucleotide

<400>  12
tagtagcgaa ctactggacc cgtccttcac c                                      31


<210>  13
<211>  54
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Anchor primer

<400>  13
ctctctctct ctctctctct ctctctctct cccttccttt ctctgagtct gtag             54


<210>  14
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Short reverse complementary oligonucleotide

<400>  14
gggaaggaaa gagactcaga catc                                              24


<210>  15
<211>  52
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Anchor primer

<400>  15
cttaagactc gatatccctg caggctctct ctctctctct ctctctctct ct               52


<210>  16
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Short reverse complementary oligonucleotide

<400>  16
gaattctgag ctatagggac gtcc                                              24


<210>  17
<211>  55
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Anchor primer


<220>
<221>  misc_feature
<222>  (1)..(54)
<223>  deoxyribonucleotide

<220>
<221>  misc_feature
<222>  (55)..(55)
<223>  ribonucleotide

<400>  17
ctctctctct ctctctctct ctctctctct ctctctctct ctctctctct ctctu            55


<210>  18
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Anchor primer


<220>
<221>  modified_base
<222>  (2)..(2)
<223>  I

<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  n is a, c, g, or t

<400>  18
anctctctct ctctctctcc tctctctctc tctctctctc tctctct                     47


<210>  19
<211>  922
<212>  DNA
<213>  Aequorea victoria

<400>  19
tacacacgaa taaaagataa caaagatgag taaaggagaa gaacttttca ctggagttgt       60

cccaattctt gttgaattag atggcgatgt taatgggcaa aaattctctg tcagtggaga      120

gggtgaaggt gatgcaacat acggaaaact tacccttaaa tttatttgca ctactgggaa      180

gctacctgtt ccatggccaa cacttgtcac tactttctct tatggtgttc aatgcttttc      240

aagataccca gatcatatga aacagcatga ctttttcaag agtgccatgc ccgaaggtta      300

tgtacaggaa agaactatat tttacaaaga tgacgggaac tacaagacac gtgctgaagt      360

caagtttgaa ggtgataccc ttgttaatag aatcgagtta aaaggtattg attttaaaga      420

agatggaaac attcttggac acaaaatgga atacaactat aactcacata atgtatacat      480

catggcagac aaaccaaaga atggaatcaa agttaacttc aaaattagac acaacattaa      540

agatggaagc gttcaattag cagaccatta tcaacaaaat actccaattg gcgatggccc      600

tgtcctttta ccagacaacc attacctgtc cacacaatct gccctttcca aagatcccaa      660

cgaaaagaga gatcacatga tccttcttga gtttgtaaca gctgctggga ttacacatgg      720

catggatgaa ctatacaaat aaatgtccag acttccaatt gacactaaag tgtccgaaca      780

attactaaat tctcagggtt cctggttaaa ttcaggctga gactttattt atatatttat      840

agattcatta aaattttatg aataatttat tgatgttatt aataggggct attttcttat      900

taaataggct actggagtgt at                                               922


<210>  20
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  N-mer

<400>  20
tacacacgaa taaaagataa c                                                 21


<210>  21
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  N-mer

<400>  21
acttttcact ggagttgtcc c                                                 21


<210>  22
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  N-mer

<400>  22
cgatgttaat gggcaaaaat t                                                 21


<210>  23
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  N-mer

<400>  23
aggtgatgca acatacggaa a                                                 21


<210>  24
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  N-mer

<400>  24
cactactggg aagctacctg t                                                 21


<210>  25
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  N-mer

<400>  25
tactttctct tatggtgttc a                                                 21


<210>  26
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  N-mer

<400>  26
tcatatgaaa cagcatgact t                                                 21


<210>  27
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  N-mer

<400>  27
aggttatgta caggaaagaa c                                                 21


<210>  28
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  N-mer

<400>  28
gaactacaag acacgtgctg a                                                 21


<210>  29
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  N-mer

<400>  29
ccttgttaat agaatcgagt t                                                 21


<210>  30
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  N-mer

<400>  30
agatggaaac attcttggac a                                                 21


<210>  31
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  N-mer

<400>  31
ctcacataat gtatacatca t                                                 21


<210>  32
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  N-mer

<400>  32
aatcaaagtt aacttcaaaa t                                                 21


<210>  33
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  N-mer

<400>  33
aagcgttcaa ttagcagacc a                                                 21


<210>  34
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  N-mer

<400>  34
tggcgatggc cctgtccttt t                                                 21


<210>  35
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  N-mer

<400>  35
cacacaatct gccctttcca a                                                 21


<210>  36
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  N-mer

<400>  36
tcacatgatc cttcttgagt t                                                 21


<210>  37
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  N-mer

<400>  37
acatggcatg gatgaactat a                                                 21


<210>  38
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  N-mer

<400>  38
aattgacact aaagtgtccg a                                                 21


<210>  39
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  N-mer

<400>  39
ttcctggtta aattcaggct g                                                 21


<210>  40
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  N-mer

<400>  40
agattcatta aaattttatg a                                                 21


<210>  41
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  N-mer

<400>  41
taggggctat tttcttatta a                                                 21


<210>  42
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  N-mer

<400>  42
aaagatgagt aaaggagaag a                                                 21


<210>  43
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  N-mer

<400>  43
aattcttgtt gaattagatg g                                                 21


<210>  44
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  N-mer

<400>  44
ctctgtcagt ggagagggtg a                                                 21


<210>  45
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  N-mer

<400>  45
acttaccctt aaatttattt g                                                 21


<210>  46
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  N-mer

<400>  46
tccatggcca acacttgtca c                                                 21


<210>  47
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  N-mer

<400>  47
atgcttttca agatacccag a                                                 21


<210>  48
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  N-mer

<400>  48
tttcaagagt gccatgcccg a                                                 21


<210>  49
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  N-mer

<400>  49
tatattttac aaagatgacg g                                                 21


<210>  50
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  N-mer

<400>  50
agtcaagttt gaaggtgata c                                                 21


<210>  51
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  N-mer

<400>  51
aaaaggtatt gattttaaag a                                                 21


<210>  52
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  N-mer

<400>  52
caaaatggaa tacaactata a                                                 21


<210>  53
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  N-mer

<400>  53
ggcagacaaa ccaaagaatg g                                                 21


<210>  54
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  N-mer

<400>  54
tagacacaac attaaagatg g                                                 21


<210>  55
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  N-mer

<400>  55
ttatcaacaa aatactccaa t                                                 21


<210>  56
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  N-mer

<400>  56
accagacaac cattacctgt c                                                 21


<210>  57
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  N-mer

<400>  57
agatcccaac gaaaagagag a                                                 21


<210>  58
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  N-mer

<400>  58
tgtaacagct gctgggatta c                                                 21


<210>  59
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  N-mer

<400>  59
caaataaatg tccagacttc c                                                 21


<210>  60
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  N-mer

<400>  60
acaattacta aattctcagg g                                                 21


<210>  61
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  N-mer

<400>  61
agactttatt tatatattta t                                                 21


<210>  62
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  N-mer

<400>  62
ataatttatt gatgttatta a                                                 21


<210>  63
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  N-mer

<400>  63
ataggctact ggagtgtat                                                    19


<210>  64
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ligation product

<400>  64
gatatcgagt cttaagtaca cacgaataaa agataac                                37


<210>  65
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ligation product

<400>  65
gatatcgagt cttaagactt ttcactggag ttgtccc                                37


<210>  66
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ligation product

<400>  66
gatatcgagt cttaagcgat gttaatgggc aaaaatt                                37


<210>  67
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ligation product

<400>  67
gatatcgagt cttaagaggt gatgcaacat acggaaa                                37


<210>  68
<211>  58
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ligation product

<400>  68
gatatcgagt cttaagtaca cacgaataaa agataacaaa gatgagtaaa ggagaaga         58


<210>  69
<211>  58
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ligation Product

<400>  69
gatatcgagt cttaagactt ttcactggag ttgtcccaat tcttgttgaa ttagatgg         58


<210>  70
<211>  58
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ligation product

<400>  70
gatatcgagt cttaagcgat gttaatgggc aaaaattctc tgtcagtgga gagggtga         58


<210>  71
<211>  57
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  71

<400>  71
gatatcgagt cttaagaggt gatgcaacat acggaaaact tacccttaaa tttattt          57


<210>  72
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Released ligation product

<400>  72
acttttcact ggagttgtcc caattcttgt tgaattagat gg                          42


<210>  73
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Released ligation product

<400>  73
aggtgatgca acatacggaa aacttaccct taaatttatt t                           41


<210>  74
<211>  100
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ligation product

<400>  74
gatatcgagt cttaagtaca cacgaataaa agataacaaa gatgagtaaa ggagaagaac       60

ttttcactgg agttgtccca attcttgttg aattagatgg                            100


<210>  75
<211>  99
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ligation product

<400>  75
gatatcgagt cttaagcgat gttaatgggc aaaaattctc tgtcagtgga gagggtgaag       60

gtgatgcaac atacggaaaa cttaccctta aatttattt                              99


<210>  76
<211>  83
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Released ligation product

<400>  76
cgatgttaat gggcaaaaat tctctgtcag tggagagggt gaaggtgatg caacatacgg       60

aaaacttacc cttaaattta ttt                                               83


<210>  77
<211>  153
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ligation product

<400>  77
gatatcgagt cttaagtaca cacgaataaa agataacaaa gatgagtaaa ggagaagaac       60

ttttcactgg agttgtccca attcttgttg aattagatgg cgatgttaat gggcaaaaat      120

tctctgtcag tggagagggt gaaggtgatg caa                                   153


<210>  78
<211>  167
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Released ligation product

<400>  78
tacacacgaa taaaagataa caaagatgag taaaggagaa gaacttttca ctggagttgt       60

cccaattctt gttgaattag atggcgatgt taatgggcaa aaattctctg tcagtggaga      120

gggtgaaggt gatgcaacat acggaaaact tacccttaaa tttattt                    167


<210>  79
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  N-mer


<220>
<221>  misc_feature
<222>  (1)..(17)
<223>  deoxyribonucleotide

<220>
<221>  misc_feature
<222>  (18)..(18)
<223>  ribonucleotide

<400>  79
ggagactcct gggacgac                                                     18


<210>  80
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  N-mer


<220>
<221>  misc_feature
<222>  (1)..(6)
<223>  deoxyribonucleotide

<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  Locked nucleic acid

<220>
<221>  misc_feature
<222>  (8)..(17)
<223>  deoxyribonucleotide

<220>
<221>  misc_feature
<222>  (18)..(18)
<223>  ribonucleotide

<400>  80
ggagactcct gggacgac                                                     18


<210>  81
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  N-mer


<220>
<221>  misc_feature
<222>  (1)..(5)
<223>  deoxyribonucleotide

<220>
<221>  misc_feature
<222>  (6)..(7)
<223>  internal Cy3 linker between deoxyribonucleotides

<220>
<221>  misc_feature
<222>  (6)..(7)
<223>  internal Cy3 linker between deoxyribonucleotides

<220>
<221>  misc_feature
<222>  (8)..(17)
<223>  deoxyribonucleotides

<220>
<221>  misc_feature
<222>  (18)..(18)
<223>  ribonucleotide

<400>  81
ggagactcct gggacgac                                                     18


