                         SEQUENCE LISTING

<110>  IPSEN BIOPHARM LIMITED
 
<120>  LABELLED POLYPEPTIDES

<130>  P60947WO

<150>  GB1900621.2
<151>  2019-01-16

<160>  123   

<170>  PatentIn version 3.5

<210>  1
<211>  7234
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of EGF-liganded polypeptide with 
       dual-labelling SrtA sites

<400>  1
tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg       60

cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc      120

ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg      180

gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc      240

acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt      300

ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc      360

ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta      420

acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt      480

cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat      540

ccgctcatga attaattctt agaaaaactc atcgagcatc aaatgaaact gcaatttatt      600

catatcagga ttatcaatac catatttttg aaaaagccgt ttctgtaatg aaggagaaaa      660

ctcaccgagg cagttccata ggatggcaag atcctggtat cggtctgcga ttccgactcg      720

tccaacatca atacaaccta ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa      780

atcaccatga gtgacgactg aatccggtga gaatggcaaa agtttatgca tttctttcca      840

gacttgttca acaggccagc cattacgctc gtcatcaaaa tcactcgcat caaccaaacc      900

gttattcatt cgtgattgcg cctgagcgag acgaaatacg cgatcgctgt taaaaggaca      960

attacaaaca ggaatcgaat gcaaccggcg caggaacact gccagcgcat caacaatatt     1020

ttcacctgaa tcaggatatt cttctaatac ctggaatgct gttttcccgg ggatcgcagt     1080

ggtgagtaac catgcatcat caggagtacg gataaaatgc ttgatggtcg gaagaggcat     1140

aaattccgtc agccagttta gtctgaccat ctcatctgta acatcattgg caacgctacc     1200

tttgccatgt ttcagaaaca actctggcgc atcgggcttc ccatacaatc gatagattgt     1260

cgcacctgat tgcccgacat tatcgcgagc ccatttatac ccatataaat cagcatccat     1320

gttggaattt aatcgcggcc tagagcaaga cgtttcccgt tgaatatggc tcataacacc     1380

ccttgtatta ctgtttatgt aagcagacag ttttattgtt catgaccaaa atcccttaac     1440

gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag     1500

atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg     1560

tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca     1620

gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga     1680

actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca     1740

gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc     1800

agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca     1860

ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa     1920

aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc     1980

cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc     2040

gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg     2100

cctttttacg gttcctggcc ttttgctggc cttttgctca catcggcgat aatggcctgc     2160

ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag     2220

attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg     2280

ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca     2340

gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg     2400

aaggctctca agggcatcgg tcgagatccc ggtgcctaat gagtgagcta acttacatta     2460

attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa     2520

tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgccaggg tggtttttct     2580

tttcaccagt gagacgggca acagctgatt gcccttcacc gcctggccct gagagagttg     2640

cagcaagcgg tccacgctgg tttgccccag caggcgaaaa tcctgtttga tggtggttaa     2700

cggcgggata taacatgagc tgtcttcggt atcgtcgtat cccactaccg agatatccgc     2760

accaacgcgc agcccggact cggtaatggc gcgcattgcg cccagcgcca tctgatcgtt     2820

ggcaaccagc atcgcagtgg gaacgatgcc ctcattcagc atttgcatgg tttgttgaaa     2880

accggacatg gcactccagt cgccttcccg ttccgctatc ggctgaattt gattgcgagt     2940

gagatattta tgccagccag ccagacgcag acgcgccgag acagaactta atgggcccgc     3000

taacagcgcg atttgctggt gacccaatgc gaccagatgc tccacgccca gtcgcgtacc     3060

gtcttcatgg gagaaaataa tactgttgat gggtgtctgg tcagagacat caagaaataa     3120

cgccggaaca ttagtgcagg cagcttccac agcaatggca tcctggtcat ccagcggata     3180

gttaatgatc agcccactga cgcgttgcgc gagaagattg tgcaccgccg ctttacaggc     3240

ttcgacgccg cttcgttcta ccatcgacac caccacgctg gcacccagtt gatcggcgcg     3300

agatttaatc gccgcgacaa tttgcgacgg cgcgtgcagg gccagactgg aggtggcaac     3360

gccaatcagc aacgactgtt tgcccgccag ttgttgtgcc acgcggttgg gaatgtaatt     3420

cagctccgcc atcgccgctt ccactttttc ccgcgttttc gcagaaacgt ggctggcctg     3480

gttcaccacg cgggaaacgg tctgataaga gacaccggca tactctgcga catcgtataa     3540

cgttactggt ttcacattca ccaccctgaa ttgactctct tccgggcgct atcatgccat     3600

accgcgaaag gttttgcgcc attcgatggt gtccgggatc tcgacgctct cccttatgcg     3660

actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa     3720

ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca     3780

tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg     3840

tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga     3900

tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa ttaatacgac tcactatagg     3960

ggaattgtga gcggataaca attcccctca agaaataatt ttgtttaact ttaagaagga     4020

gatatacata tgggatccat ggagaacctg tattttcagg gcggcggtgg cagcggcggc     4080

agcggcggca gccctttcgt taacaaacag ttcaactata aagacccagt taacggtgtt     4140

gacattgctt acatcaaaat cccgaacgct ggccagatgc agccggtaaa ggcattcaaa     4200

atccacaaca aaatctgggt tatcccggaa cgtgatacct ttactaaccc ggaagaaggt     4260

gacctgaacc cgccaccgga agcgaaacag gtgccggtat cttactatga ctccacctac     4320

ctgtctaccg ataacgaaaa ggacaactac ctgaaaggtg ttactaaact gttcgagcgt     4380

atttactcca ccgacctggg ccgtatgctg ctgactagca tcgttcgcgg tatcccgttc     4440

tggggcggtt ctaccatcga taccgaactg aaagtaatcg acactaactg catcaacgtt     4500

attcagccgg acggttccta tcgttccgaa gaactgaacc tggtgatcat cggcccgtct     4560

gctgatatca tccagttcga gtgtaagagc tttggtcacg aagttctgaa cctcacccgt     4620

aacggctacg gttccactca gtacatccgt ttctctccgg acttcacctt cggttttgaa     4680

gaatccctgg aagtagacac gaacccactg ctgggcgctg gtaaattcgc aactgatcct     4740

gcggttaccc tggctcacga actgattcat gcaggccacc gcctgtacgg tatcgccatc     4800

aatccgaacc gtgtcttcaa agttaacacc aacgcgtatt acgagatgtc cggtctggaa     4860

gttagcttcg aagaactgcg tacttttggc ggtcacgacg ctaaattcat cgactctctg     4920

caagaaaacg agttccgtct gtactactat aacaagttca aagatatcgc atccaccctg     4980

aacaaagcga aatccatcgt gggtaccact gcttctctcc agtacatgaa gaacgttttt     5040

aaagaaaaat acctgctcag cgaagacacc tccggcaaat tctctgtaga caagttgaaa     5100

ttcgataaac tttacaaaat gctgactgaa atttacaccg aagacaactt cgttaagttc     5160

tttaaagttc tgaaccgcaa aacctatctg aacttcgaca aggcagtatt caaaatcaac     5220

atcgtgccga aagttaacta cactatctac gatggtttca acctgcgtaa caccaacctg     5280

gctgctaatt ttaacggcca gaacacggaa atcaacaaca tgaacttcac aaaactgaaa     5340

aacttcactg gtctgttcga gttttacaag ctgctgtgcg tcgacggcat cattacctcc     5400

aaaactaaat ctctgataga aggtagaaac aaagcgctga acctgcagtg tatcaaggtt     5460

aacaactggg atttattctt cagcccgagt gaagacaact tcaccaacga cctgaacaaa     5520

ggtgaagaaa tcacctcaga tactaacatc gaagcagccg aagaaaacat ctcgctggac     5580

ctgatccagc agtactacct gacctttaat ttcgacaacg agccggaaaa catttctatc     5640

gaaaacctga gctctgatat catcggccag ctggaactga tgccgaacat cgaacgtttc     5700

ccaaacggta aaaagtacga gctggacaaa tataccatgt tccactacct gcgcgcgcag     5760

gaatttgaac acggcaaatc ccgtatcgca ctgactaact ccgttaacga agctctgctc     5820

aacccgtccc gtgtatacac cttcttctct agcgactacg tgaaaaaggt caacaaagcg     5880

actgaagctg caatgttctt gggttgggtt gaacagcttg tttatgattt taccgacgag     5940

acgtccgaag tatctactac cgacaaaatt gcggatatca ctatcatcat cccgtacatc     6000

ggtccggctc tgaacattgg caacatgctg tacaaagacg acttcgttgg cgcactgatc     6060

ttctccggtg cggtgatcct gctggagttc atcccggaaa tcgccatccc ggtactgggc     6120

acctttgctc tggtttctta cattgcaaac aaggttctga ctgtacaaac catcgacaac     6180

gcgctgagca aacgtaacga aaaatgggat gaagtttaca aatatatcgt gaccaactgg     6240

ctggctaagg ttaatactca gatcgacctc atccgcaaaa aaatgaaaga agcactggaa     6300

aaccaggcgg aagctaccaa ggcaatcatt aactaccagt acaaccagta caccgaggaa     6360

gaaaaaaaca acatcaactt caacatcgac gatctgtcct ctaaactgaa cgaatccatc     6420

aacaaagcta tgatcaacat caacaagttc ctgaaccagt gctctgtaag ctatctgatg     6480

aactccatga tcccgtacgg tgttaaacgt ctggaggact tcgatgcgtc tctgaaagac     6540

gccctgctga aatacattta cgacaaccgt ggcactctga tcggtcaggt tgatcgtctg     6600

aaggacaaag tgaacaatac cttatcgacc gacatccctt ttcagctcag taaatatgtc     6660

gataaccaac gccttttgtc cactctagaa ggcggtggcg gtagcggtgg cggtggcagc     6720

ggcggtggcg gtagcgcact agacaacagc gaccctaaat gcccactgag tcatgaagga     6780

tactgcctta atgatggtgt ttgtatgtac ataggaacat tggaccgtta tgcttgcaat     6840

tgtgtagtgg gctatgtcgg ggaaaggtgt caatatcgag atctcaagct ggcagagtta     6900

agagggctag aagcaggcgg cagcggcggc ggcagcggcc tgcccgaaag cggtggcgga     6960

tctgcttggt ctcacccgca gttcgaaaaa ggtggtggtt ctggtggtgg ttctggtggt     7020

tctgcttggt ctcacccgca gttcgaaaaa taatgaaagc ttgcggccgc actcgagcac     7080

caccaccacc accactgaga tccggctgct aacaaagccc gaaaggaagc tgagttggct     7140

gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg     7200

ggttttttgc tgaaaggagg aactatatcc ggat                                 7234


<210>  2
<211>  1004
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Polypeptide sequence of EGF-liganded polypeptide with 
       dual-labelling SrtA sites

<400>  2

Met Glu Asn Leu Tyr Phe Gln Gly Gly Gly Gly Ser Gly Gly Ser Gly 
1               5                   10                  15      


Gly Ser Pro Phe Val Asn Lys Gln Phe Asn Tyr Lys Asp Pro Val Asn 
            20                  25                  30          


Gly Val Asp Ile Ala Tyr Ile Lys Ile Pro Asn Ala Gly Gln Met Gln 
        35                  40                  45              


Pro Val Lys Ala Phe Lys Ile His Asn Lys Ile Trp Val Ile Pro Glu 
    50                  55                  60                  


Arg Asp Thr Phe Thr Asn Pro Glu Glu Gly Asp Leu Asn Pro Pro Pro 
65                  70                  75                  80  


Glu Ala Lys Gln Val Pro Val Ser Tyr Tyr Asp Ser Thr Tyr Leu Ser 
                85                  90                  95      


Thr Asp Asn Glu Lys Asp Asn Tyr Leu Lys Gly Val Thr Lys Leu Phe 
            100                 105                 110         


Glu Arg Ile Tyr Ser Thr Asp Leu Gly Arg Met Leu Leu Thr Ser Ile 
        115                 120                 125             


Val Arg Gly Ile Pro Phe Trp Gly Gly Ser Thr Ile Asp Thr Glu Leu 
    130                 135                 140                 


Lys Val Ile Asp Thr Asn Cys Ile Asn Val Ile Gln Pro Asp Gly Ser 
145                 150                 155                 160 


Tyr Arg Ser Glu Glu Leu Asn Leu Val Ile Ile Gly Pro Ser Ala Asp 
                165                 170                 175     


Ile Ile Gln Phe Glu Cys Lys Ser Phe Gly His Glu Val Leu Asn Leu 
            180                 185                 190         


Thr Arg Asn Gly Tyr Gly Ser Thr Gln Tyr Ile Arg Phe Ser Pro Asp 
        195                 200                 205             


Phe Thr Phe Gly Phe Glu Glu Ser Leu Glu Val Asp Thr Asn Pro Leu 
    210                 215                 220                 


Leu Gly Ala Gly Lys Phe Ala Thr Asp Pro Ala Val Thr Leu Ala His 
225                 230                 235                 240 


Glu Leu Ile His Ala Gly His Arg Leu Tyr Gly Ile Ala Ile Asn Pro 
                245                 250                 255     


Asn Arg Val Phe Lys Val Asn Thr Asn Ala Tyr Tyr Glu Met Ser Gly 
            260                 265                 270         


Leu Glu Val Ser Phe Glu Glu Leu Arg Thr Phe Gly Gly His Asp Ala 
        275                 280                 285             


Lys Phe Ile Asp Ser Leu Gln Glu Asn Glu Phe Arg Leu Tyr Tyr Tyr 
    290                 295                 300                 


Asn Lys Phe Lys Asp Ile Ala Ser Thr Leu Asn Lys Ala Lys Ser Ile 
305                 310                 315                 320 


Val Gly Thr Thr Ala Ser Leu Gln Tyr Met Lys Asn Val Phe Lys Glu 
                325                 330                 335     


Lys Tyr Leu Leu Ser Glu Asp Thr Ser Gly Lys Phe Ser Val Asp Lys 
            340                 345                 350         


Leu Lys Phe Asp Lys Leu Tyr Lys Met Leu Thr Glu Ile Tyr Thr Glu 
        355                 360                 365             


Asp Asn Phe Val Lys Phe Phe Lys Val Leu Asn Arg Lys Thr Tyr Leu 
    370                 375                 380                 


Asn Phe Asp Lys Ala Val Phe Lys Ile Asn Ile Val Pro Lys Val Asn 
385                 390                 395                 400 


Tyr Thr Ile Tyr Asp Gly Phe Asn Leu Arg Asn Thr Asn Leu Ala Ala 
                405                 410                 415     


Asn Phe Asn Gly Gln Asn Thr Glu Ile Asn Asn Met Asn Phe Thr Lys 
            420                 425                 430         


Leu Lys Asn Phe Thr Gly Leu Phe Glu Phe Tyr Lys Leu Leu Cys Val 
        435                 440                 445             


Asp Gly Ile Ile Thr Ser Lys Thr Lys Ser Leu Ile Glu Gly Arg Asn 
    450                 455                 460                 


Lys Ala Leu Asn Leu Gln Cys Ile Lys Val Asn Asn Trp Asp Leu Phe 
465                 470                 475                 480 


Phe Ser Pro Ser Glu Asp Asn Phe Thr Asn Asp Leu Asn Lys Gly Glu 
                485                 490                 495     


Glu Ile Thr Ser Asp Thr Asn Ile Glu Ala Ala Glu Glu Asn Ile Ser 
            500                 505                 510         


Leu Asp Leu Ile Gln Gln Tyr Tyr Leu Thr Phe Asn Phe Asp Asn Glu 
        515                 520                 525             


Pro Glu Asn Ile Ser Ile Glu Asn Leu Ser Ser Asp Ile Ile Gly Gln 
    530                 535                 540                 


Leu Glu Leu Met Pro Asn Ile Glu Arg Phe Pro Asn Gly Lys Lys Tyr 
545                 550                 555                 560 


Glu Leu Asp Lys Tyr Thr Met Phe His Tyr Leu Arg Ala Gln Glu Phe 
                565                 570                 575     


Glu His Gly Lys Ser Arg Ile Ala Leu Thr Asn Ser Val Asn Glu Ala 
            580                 585                 590         


Leu Leu Asn Pro Ser Arg Val Tyr Thr Phe Phe Ser Ser Asp Tyr Val 
        595                 600                 605             


Lys Lys Val Asn Lys Ala Thr Glu Ala Ala Met Phe Leu Gly Trp Val 
    610                 615                 620                 


Glu Gln Leu Val Tyr Asp Phe Thr Asp Glu Thr Ser Glu Val Ser Thr 
625                 630                 635                 640 


Thr Asp Lys Ile Ala Asp Ile Thr Ile Ile Ile Pro Tyr Ile Gly Pro 
                645                 650                 655     


Ala Leu Asn Ile Gly Asn Met Leu Tyr Lys Asp Asp Phe Val Gly Ala 
            660                 665                 670         


Leu Ile Phe Ser Gly Ala Val Ile Leu Leu Glu Phe Ile Pro Glu Ile 
        675                 680                 685             


Ala Ile Pro Val Leu Gly Thr Phe Ala Leu Val Ser Tyr Ile Ala Asn 
    690                 695                 700                 


Lys Val Leu Thr Val Gln Thr Ile Asp Asn Ala Leu Ser Lys Arg Asn 
705                 710                 715                 720 


Glu Lys Trp Asp Glu Val Tyr Lys Tyr Ile Val Thr Asn Trp Leu Ala 
                725                 730                 735     


Lys Val Asn Thr Gln Ile Asp Leu Ile Arg Lys Lys Met Lys Glu Ala 
            740                 745                 750         


Leu Glu Asn Gln Ala Glu Ala Thr Lys Ala Ile Ile Asn Tyr Gln Tyr 
        755                 760                 765             


Asn Gln Tyr Thr Glu Glu Glu Lys Asn Asn Ile Asn Phe Asn Ile Asp 
    770                 775                 780                 


Asp Leu Ser Ser Lys Leu Asn Glu Ser Ile Asn Lys Ala Met Ile Asn 
785                 790                 795                 800 


Ile Asn Lys Phe Leu Asn Gln Cys Ser Val Ser Tyr Leu Met Asn Ser 
                805                 810                 815     


Met Ile Pro Tyr Gly Val Lys Arg Leu Glu Asp Phe Asp Ala Ser Leu 
            820                 825                 830         


Lys Asp Ala Leu Leu Lys Tyr Ile Tyr Asp Asn Arg Gly Thr Leu Ile 
        835                 840                 845             


Gly Gln Val Asp Arg Leu Lys Asp Lys Val Asn Asn Thr Leu Ser Thr 
    850                 855                 860                 


Asp Ile Pro Phe Gln Leu Ser Lys Tyr Val Asp Asn Gln Arg Leu Leu 
865                 870                 875                 880 


Ser Thr Leu Glu Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
                885                 890                 895     


Gly Gly Ser Ala Leu Asp Asn Ser Asp Pro Lys Cys Pro Leu Ser His 
            900                 905                 910         


Glu Gly Tyr Cys Leu Asn Asp Gly Val Cys Met Tyr Ile Gly Thr Leu 
        915                 920                 925             


Asp Arg Tyr Ala Cys Asn Cys Val Val Gly Tyr Val Gly Glu Arg Cys 
    930                 935                 940                 


Gln Tyr Arg Asp Leu Lys Leu Ala Glu Leu Arg Gly Leu Glu Ala Gly 
945                 950                 955                 960 


Gly Ser Gly Gly Gly Ser Gly Leu Pro Glu Ser Gly Gly Gly Ser Ala 
                965                 970                 975     


Trp Ser His Pro Gln Phe Glu Lys Gly Gly Gly Ser Gly Gly Gly Ser 
            980                 985                 990         


Gly Gly Ser Ala Trp Ser His Pro  Gln Phe Glu Lys 
        995                 1000                 


<210>  3
<211>  7108
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of nociceptin-liganded polypeptide with 
       dual-labelling SrtA sites

<400>  3
tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg       60

cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc      120

ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg      180

gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc      240

acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt      300

ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc      360

ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta      420

acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt      480

cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat      540

ccgctcatga attaattctt agaaaaactc atcgagcatc aaatgaaact gcaatttatt      600

catatcagga ttatcaatac catatttttg aaaaagccgt ttctgtaatg aaggagaaaa      660

ctcaccgagg cagttccata ggatggcaag atcctggtat cggtctgcga ttccgactcg      720

tccaacatca atacaaccta ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa      780

atcaccatga gtgacgactg aatccggtga gaatggcaaa agtttatgca tttctttcca      840

gacttgttca acaggccagc cattacgctc gtcatcaaaa tcactcgcat caaccaaacc      900

gttattcatt cgtgattgcg cctgagcgag acgaaatacg cgatcgctgt taaaaggaca      960

attacaaaca ggaatcgaat gcaaccggcg caggaacact gccagcgcat caacaatatt     1020

ttcacctgaa tcaggatatt cttctaatac ctggaatgct gttttcccgg ggatcgcagt     1080

ggtgagtaac catgcatcat caggagtacg gataaaatgc ttgatggtcg gaagaggcat     1140

aaattccgtc agccagttta gtctgaccat ctcatctgta acatcattgg caacgctacc     1200

tttgccatgt ttcagaaaca actctggcgc atcgggcttc ccatacaatc gatagattgt     1260

cgcacctgat tgcccgacat tatcgcgagc ccatttatac ccatataaat cagcatccat     1320

gttggaattt aatcgcggcc tagagcaaga cgtttcccgt tgaatatggc tcataacacc     1380

ccttgtatta ctgtttatgt aagcagacag ttttattgtt catgaccaaa atcccttaac     1440

gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag     1500

atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg     1560

tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca     1620

gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga     1680

actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca     1740

gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc     1800

agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca     1860

ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa     1920

aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc     1980

cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc     2040

gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg     2100

cctttttacg gttcctggcc ttttgctggc cttttgctca catcggcgat aatggcctgc     2160

ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag     2220

attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg     2280

ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca     2340

gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg     2400

aaggctctca agggcatcgg tcgagatccc ggtgcctaat gagtgagcta acttacatta     2460

attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa     2520

tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgccaggg tggtttttct     2580

tttcaccagt gagacgggca acagctgatt gcccttcacc gcctggccct gagagagttg     2640

cagcaagcgg tccacgctgg tttgccccag caggcgaaaa tcctgtttga tggtggttaa     2700

cggcgggata taacatgagc tgtcttcggt atcgtcgtat cccactaccg agatatccgc     2760

accaacgcgc agcccggact cggtaatggc gcgcattgcg cccagcgcca tctgatcgtt     2820

ggcaaccagc atcgcagtgg gaacgatgcc ctcattcagc atttgcatgg tttgttgaaa     2880

accggacatg gcactccagt cgccttcccg ttccgctatc ggctgaattt gattgcgagt     2940

gagatattta tgccagccag ccagacgcag acgcgccgag acagaactta atgggcccgc     3000

taacagcgcg atttgctggt gacccaatgc gaccagatgc tccacgccca gtcgcgtacc     3060

gtcttcatgg gagaaaataa tactgttgat gggtgtctgg tcagagacat caagaaataa     3120

cgccggaaca ttagtgcagg cagcttccac agcaatggca tcctggtcat ccagcggata     3180

gttaatgatc agcccactga cgcgttgcgc gagaagattg tgcaccgccg ctttacaggc     3240

ttcgacgccg cttcgttcta ccatcgacac caccacgctg gcacccagtt gatcggcgcg     3300

agatttaatc gccgcgacaa tttgcgacgg cgcgtgcagg gccagactgg aggtggcaac     3360

gccaatcagc aacgactgtt tgcccgccag ttgttgtgcc acgcggttgg gaatgtaatt     3420

cagctccgcc atcgccgctt ccactttttc ccgcgttttc gcagaaacgt ggctggcctg     3480

gttcaccacg cgggaaacgg tctgataaga gacaccggca tactctgcga catcgtataa     3540

cgttactggt ttcacattca ccaccctgaa ttgactctct tccgggcgct atcatgccat     3600

accgcgaaag gttttgcgcc attcgatggt gtccgggatc tcgacgctct cccttatgcg     3660

actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa     3720

ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca     3780

tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg     3840

tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga     3900

tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa ttaatacgac tcactatagg     3960

ggaattgtga gcggataaca attcccctca agaaataatt ttgtttaact ttaagaagga     4020

gatatacata tggagaacct gtattttcag ggcggcggtg gcagcggcgg cagcggcggc     4080

agcggcagca tgccttttgt gaacaaacag ttcaactata aggatccggt taatggtgtg     4140

gatatcgcct atatcaaaat tccgaatgca ggtcagatgc agccggttaa agcctttaaa     4200

atccataaca aaatttgggt gattccggaa cgtgatacct ttaccaatcc ggaagaaggt     4260

gatctgaatc cgcctccgga agcaaaacag gttccggtta gctattatga tagcacctat     4320

ctgagcaccg ataacgagaa agataactat ctgaaaggtg tgaccaaact gtttgaacgc     4380

atttatagta ccgatctggg tcgtatgctg ctgaccagca ttgttcgtgg tattccgttt     4440

tggggtggta gcaccattga taccgaactg aaagttattg acaccaactg cattaatgtg     4500

attcagccgg atggtagcta tcgtagcgaa gaactgaatc tggttattat tggtccgagc     4560

gcagatatca ttcagtttga atgtaaatcc tttggccacg aagttctgaa tctgacccgt     4620

aatggttatg gtagtaccca gtatattcgt ttcagtccgg attttacctt tggctttgaa     4680

gaaagcctgg aagttgatac aaatccgctg ttaggtgcag gtaaatttgc aaccgatccg     4740

gcagttaccc tggcacatga actgattcat gccggtcatc gtctgtatgg tattgcaatt     4800

aatccgaacc gtgtgttcaa agtgaatacc aacgcatatt atgaaatgag cggtctggaa     4860

gtgtcatttg aagaactgcg tacctttggt ggtcatgatg ccaaatttat cgatagcctg     4920

caagaaaatg aatttcgcct gtactactat aacaaattca aggatattgc gagcaccctg     4980

aataaagcca aaagcattgt tggcaccacc gcaagcctgc agtatatgaa aaatgtgttt     5040

aaagaaaaat atctgctgag cgaagatacc agcggtaaat ttagcgttga caaactgaaa     5100

ttcgataaac tgtacaagat gctgaccgag atttataccg aagataactt cgtgaagttt     5160

ttcaaagtgc tgaaccgcaa aacctacctg aactttgata aagccgtgtt caaaatcaac     5220

atcgtgccga aagtgaacta taccatctat gatggtttta acctgcgcaa taccaatctg     5280

gcagcaaact ttaatggtca gaacaccgaa atcaacaaca tgaactttac caaactgaag     5340

aacttcaccg gtctgttcga attttacaaa ctgctgtgtg tggatggcat tattaccagc     5400

aaaaccaaat ccgatgatga cgataaattc ggtggtttta ccggtgcacg taaaagcgca     5460

cgtaaacgta aaaatcaggc actggcaggc ggtggtggta gcggtggcgg tggttcaggt     5520

ggtggtggct cagcactggt tctgcagtgt attaaagtta ataactggga cctgtttttt     5580

agcccgagcg aggataattt caccaacgat ctgaacaaag gcgaagaaat taccagcgat     5640

accaatattg aagcagccga agaaaacatt agcctggatc tgattcagca gtattatctg     5700

accttcaact tcgataatga gccggaaaat atcagcattg aaaacctgag cagcgatatt     5760

attggccagc tggaactgat gccgaatatt gaacgttttc cgaacggcaa aaaatacgag     5820

ctggataaat acaccatgtt ccattatctg cgtgcccaag aatttgaaca tggtaaaagc     5880

cgtattgcac tgaccaatag cgttaatgaa gcactgctga acccgagccg tgtttatacc     5940

ttttttagca gcgattacgt gaaaaaggtt aacaaagcaa ccgaagcagc catgttttta     6000

ggttgggttg aacagctggt ttatgatttc accgatgaaa ccagcgaagt tagcaccacc     6060

gataaaattg cagatattac catcatcatc ccgtatatcg gtccggcact gaatattggc     6120

aatatgctgt ataaagacga ttttgtgggt gccctgatct ttagcggtgc agttattctg     6180

ctggaattta ttccggaaat tgccattccg gttctgggca cctttgcact ggtgagctat     6240

attgcaaata aagttctgac cgtgcagacc atcgataatg cactgagcaa acgtaacgaa     6300

aaatgggatg aagtgtacaa gtatatcgtg accaattggc tggcaaaagt taacacccag     6360

attgacctga ttcgcaagaa gatgaaagaa gcactggaaa accaggcaga agcaaccaaa     6420

gccattatta actatcagta caaccagtac accgaagaag agaagaataa catcaacttc     6480

aacatcgatg atctgagcag caagctgaat gaaagcatca acaaagccat gatcaacatt     6540

aacaaatttc tgaatcagtg cagcgtgagc tatctgatga atagcatgat tccgtatggt     6600

gtgaaacgtc tggaagattt tgatgcaagc ctgaaagatg ccctgctgaa atatatctat     6660

gataatcgtg gcaccctgat tggtcaggtt gatcgtctga aagataaagt gaacaacacc     6720

ctgagtaccg atattccttt tcagctgagc aaatatgtgg ataatcagcg tctgctgagt     6780

accctggatg gcggcagcgg cggcggcagc ggcctgcccg aaagcggtgg cggatctgct     6840

tggtctcacc cgcagttcga aaaaggtggt ggttctggtg gtggttctgg tggttctgct     6900

tggtctcacc cgcagttcga aaaataatga aagcttgcgg ccgcactcga gcaccaccac     6960

caccaccact gagatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc     7020

accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt     7080

ttgctgaaag gaggaactat atccggat                                        7108


<210>  4
<211>  948
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Polypeptide sequence of nociceptin-liganded polypeptide with 
       dual-labelling SrtA sites

<400>  4

Met Glu Asn Leu Tyr Phe Gln Gly Gly Gly Gly Ser Gly Gly Ser Gly 
1               5                   10                  15      


Gly Ser Gly Ser Met Pro Phe Val Asn Lys Gln Phe Asn Tyr Lys Asp 
            20                  25                  30          


Pro Val Asn Gly Val Asp Ile Ala Tyr Ile Lys Ile Pro Asn Ala Gly 
        35                  40                  45              


Gln Met Gln Pro Val Lys Ala Phe Lys Ile His Asn Lys Ile Trp Val 
    50                  55                  60                  


Ile Pro Glu Arg Asp Thr Phe Thr Asn Pro Glu Glu Gly Asp Leu Asn 
65                  70                  75                  80  


Pro Pro Pro Glu Ala Lys Gln Val Pro Val Ser Tyr Tyr Asp Ser Thr 
                85                  90                  95      


Tyr Leu Ser Thr Asp Asn Glu Lys Asp Asn Tyr Leu Lys Gly Val Thr 
            100                 105                 110         


Lys Leu Phe Glu Arg Ile Tyr Ser Thr Asp Leu Gly Arg Met Leu Leu 
        115                 120                 125             


Thr Ser Ile Val Arg Gly Ile Pro Phe Trp Gly Gly Ser Thr Ile Asp 
    130                 135                 140                 


Thr Glu Leu Lys Val Ile Asp Thr Asn Cys Ile Asn Val Ile Gln Pro 
145                 150                 155                 160 


Asp Gly Ser Tyr Arg Ser Glu Glu Leu Asn Leu Val Ile Ile Gly Pro 
                165                 170                 175     


Ser Ala Asp Ile Ile Gln Phe Glu Cys Lys Ser Phe Gly His Glu Val 
            180                 185                 190         


Leu Asn Leu Thr Arg Asn Gly Tyr Gly Ser Thr Gln Tyr Ile Arg Phe 
        195                 200                 205             


Ser Pro Asp Phe Thr Phe Gly Phe Glu Glu Ser Leu Glu Val Asp Thr 
    210                 215                 220                 


Asn Pro Leu Leu Gly Ala Gly Lys Phe Ala Thr Asp Pro Ala Val Thr 
225                 230                 235                 240 


Leu Ala His Glu Leu Ile His Ala Gly His Arg Leu Tyr Gly Ile Ala 
                245                 250                 255     


Ile Asn Pro Asn Arg Val Phe Lys Val Asn Thr Asn Ala Tyr Tyr Glu 
            260                 265                 270         


Met Ser Gly Leu Glu Val Ser Phe Glu Glu Leu Arg Thr Phe Gly Gly 
        275                 280                 285             


His Asp Ala Lys Phe Ile Asp Ser Leu Gln Glu Asn Glu Phe Arg Leu 
    290                 295                 300                 


Tyr Tyr Tyr Asn Lys Phe Lys Asp Ile Ala Ser Thr Leu Asn Lys Ala 
305                 310                 315                 320 


Lys Ser Ile Val Gly Thr Thr Ala Ser Leu Gln Tyr Met Lys Asn Val 
                325                 330                 335     


Phe Lys Glu Lys Tyr Leu Leu Ser Glu Asp Thr Ser Gly Lys Phe Ser 
            340                 345                 350         


Val Asp Lys Leu Lys Phe Asp Lys Leu Tyr Lys Met Leu Thr Glu Ile 
        355                 360                 365             


Tyr Thr Glu Asp Asn Phe Val Lys Phe Phe Lys Val Leu Asn Arg Lys 
    370                 375                 380                 


Thr Tyr Leu Asn Phe Asp Lys Ala Val Phe Lys Ile Asn Ile Val Pro 
385                 390                 395                 400 


Lys Val Asn Tyr Thr Ile Tyr Asp Gly Phe Asn Leu Arg Asn Thr Asn 
                405                 410                 415     


Leu Ala Ala Asn Phe Asn Gly Gln Asn Thr Glu Ile Asn Asn Met Asn 
            420                 425                 430         


Phe Thr Lys Leu Lys Asn Phe Thr Gly Leu Phe Glu Phe Tyr Lys Leu 
        435                 440                 445             


Leu Cys Val Asp Gly Ile Ile Thr Ser Lys Thr Lys Ser Asp Asp Asp 
    450                 455                 460                 


Asp Lys Phe Gly Gly Phe Thr Gly Ala Arg Lys Ser Ala Arg Lys Arg 
465                 470                 475                 480 


Lys Asn Gln Ala Leu Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
                485                 490                 495     


Gly Gly Gly Gly Ser Ala Leu Val Leu Gln Cys Ile Lys Val Asn Asn 
            500                 505                 510         


Trp Asp Leu Phe Phe Ser Pro Ser Glu Asp Asn Phe Thr Asn Asp Leu 
        515                 520                 525             


Asn Lys Gly Glu Glu Ile Thr Ser Asp Thr Asn Ile Glu Ala Ala Glu 
    530                 535                 540                 


Glu Asn Ile Ser Leu Asp Leu Ile Gln Gln Tyr Tyr Leu Thr Phe Asn 
545                 550                 555                 560 


Phe Asp Asn Glu Pro Glu Asn Ile Ser Ile Glu Asn Leu Ser Ser Asp 
                565                 570                 575     


Ile Ile Gly Gln Leu Glu Leu Met Pro Asn Ile Glu Arg Phe Pro Asn 
            580                 585                 590         


Gly Lys Lys Tyr Glu Leu Asp Lys Tyr Thr Met Phe His Tyr Leu Arg 
        595                 600                 605             


Ala Gln Glu Phe Glu His Gly Lys Ser Arg Ile Ala Leu Thr Asn Ser 
    610                 615                 620                 


Val Asn Glu Ala Leu Leu Asn Pro Ser Arg Val Tyr Thr Phe Phe Ser 
625                 630                 635                 640 


Ser Asp Tyr Val Lys Lys Val Asn Lys Ala Thr Glu Ala Ala Met Phe 
                645                 650                 655     


Leu Gly Trp Val Glu Gln Leu Val Tyr Asp Phe Thr Asp Glu Thr Ser 
            660                 665                 670         


Glu Val Ser Thr Thr Asp Lys Ile Ala Asp Ile Thr Ile Ile Ile Pro 
        675                 680                 685             


Tyr Ile Gly Pro Ala Leu Asn Ile Gly Asn Met Leu Tyr Lys Asp Asp 
    690                 695                 700                 


Phe Val Gly Ala Leu Ile Phe Ser Gly Ala Val Ile Leu Leu Glu Phe 
705                 710                 715                 720 


Ile Pro Glu Ile Ala Ile Pro Val Leu Gly Thr Phe Ala Leu Val Ser 
                725                 730                 735     


Tyr Ile Ala Asn Lys Val Leu Thr Val Gln Thr Ile Asp Asn Ala Leu 
            740                 745                 750         


Ser Lys Arg Asn Glu Lys Trp Asp Glu Val Tyr Lys Tyr Ile Val Thr 
        755                 760                 765             


Asn Trp Leu Ala Lys Val Asn Thr Gln Ile Asp Leu Ile Arg Lys Lys 
    770                 775                 780                 


Met Lys Glu Ala Leu Glu Asn Gln Ala Glu Ala Thr Lys Ala Ile Ile 
785                 790                 795                 800 


Asn Tyr Gln Tyr Asn Gln Tyr Thr Glu Glu Glu Lys Asn Asn Ile Asn 
                805                 810                 815     


Phe Asn Ile Asp Asp Leu Ser Ser Lys Leu Asn Glu Ser Ile Asn Lys 
            820                 825                 830         


Ala Met Ile Asn Ile Asn Lys Phe Leu Asn Gln Cys Ser Val Ser Tyr 
        835                 840                 845             


Leu Met Asn Ser Met Ile Pro Tyr Gly Val Lys Arg Leu Glu Asp Phe 
    850                 855                 860                 


Asp Ala Ser Leu Lys Asp Ala Leu Leu Lys Tyr Ile Tyr Asp Asn Arg 
865                 870                 875                 880 


Gly Thr Leu Ile Gly Gln Val Asp Arg Leu Lys Asp Lys Val Asn Asn 
                885                 890                 895     


Thr Leu Ser Thr Asp Ile Pro Phe Gln Leu Ser Lys Tyr Val Asp Asn 
            900                 905                 910         


Gln Arg Leu Leu Ser Thr Leu Asp Gly Gly Ser Gly Gly Gly Ser Gly 
        915                 920                 925             


Leu Pro Glu Ser Gly Gly Gly Ser Ala Trp Ser His Pro Gln Phe Glu 
    930                 935                 940                 


Lys Gly Gly Gly 
945             


<210>  5
<211>  7078
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of EGF-liganded polypeptide

<400>  5
tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg       60

cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc      120

ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg      180

gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc      240

acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt      300

ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc      360

ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta      420

acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt      480

cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat      540

ccgctcatga attaattctt agaaaaactc atcgagcatc aaatgaaact gcaatttatt      600

catatcagga ttatcaatac catatttttg aaaaagccgt ttctgtaatg aaggagaaaa      660

ctcaccgagg cagttccata ggatggcaag atcctggtat cggtctgcga ttccgactcg      720

tccaacatca atacaaccta ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa      780

atcaccatga gtgacgactg aatccggtga gaatggcaaa agtttatgca tttctttcca      840

gacttgttca acaggccagc cattacgctc gtcatcaaaa tcactcgcat caaccaaacc      900

gttattcatt cgtgattgcg cctgagcgag acgaaatacg cgatcgctgt taaaaggaca      960

attacaaaca ggaatcgaat gcaaccggcg caggaacact gccagcgcat caacaatatt     1020

ttcacctgaa tcaggatatt cttctaatac ctggaatgct gttttcccgg ggatcgcagt     1080

ggtgagtaac catgcatcat caggagtacg gataaaatgc ttgatggtcg gaagaggcat     1140

aaattccgtc agccagttta gtctgaccat ctcatctgta acatcattgg caacgctacc     1200

tttgccatgt ttcagaaaca actctggcgc atcgggcttc ccatacaatc gatagattgt     1260

cgcacctgat tgcccgacat tatcgcgagc ccatttatac ccatataaat cagcatccat     1320

gttggaattt aatcgcggcc tagagcaaga cgtttcccgt tgaatatggc tcataacacc     1380

ccttgtatta ctgtttatgt aagcagacag ttttattgtt catgaccaaa atcccttaac     1440

gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag     1500

atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg     1560

tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca     1620

gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga     1680

actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca     1740

gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc     1800

agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca     1860

ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa     1920

aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc     1980

cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc     2040

gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg     2100

cctttttacg gttcctggcc ttttgctggc cttttgctca catcggcgat aatggcctgc     2160

ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag     2220

attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg     2280

ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca     2340

gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg     2400

aaggctctca agggcatcgg tcgagatccc ggtgcctaat gagtgagcta acttacatta     2460

attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa     2520

tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgccaggg tggtttttct     2580

tttcaccagt gagacgggca acagctgatt gcccttcacc gcctggccct gagagagttg     2640

cagcaagcgg tccacgctgg tttgccccag caggcgaaaa tcctgtttga tggtggttaa     2700

cggcgggata taacatgagc tgtcttcggt atcgtcgtat cccactaccg agatatccgc     2760

accaacgcgc agcccggact cggtaatggc gcgcattgcg cccagcgcca tctgatcgtt     2820

ggcaaccagc atcgcagtgg gaacgatgcc ctcattcagc atttgcatgg tttgttgaaa     2880

accggacatg gcactccagt cgccttcccg ttccgctatc ggctgaattt gattgcgagt     2940

gagatattta tgccagccag ccagacgcag acgcgccgag acagaactta atgggcccgc     3000

taacagcgcg atttgctggt gacccaatgc gaccagatgc tccacgccca gtcgcgtacc     3060

gtcttcatgg gagaaaataa tactgttgat gggtgtctgg tcagagacat caagaaataa     3120

cgccggaaca ttagtgcagg cagcttccac agcaatggca tcctggtcat ccagcggata     3180

gttaatgatc agcccactga cgcgttgcgc gagaagattg tgcaccgccg ctttacaggc     3240

ttcgacgccg cttcgttcta ccatcgacac caccacgctg gcacccagtt gatcggcgcg     3300

agatttaatc gccgcgacaa tttgcgacgg cgcgtgcagg gccagactgg aggtggcaac     3360

gccaatcagc aacgactgtt tgcccgccag ttgttgtgcc acgcggttgg gaatgtaatt     3420

cagctccgcc atcgccgctt ccactttttc ccgcgttttc gcagaaacgt ggctggcctg     3480

gttcaccacg cgggaaacgg tctgataaga gacaccggca tactctgcga catcgtataa     3540

cgttactggt ttcacattca ccaccctgaa ttgactctct tccgggcgct atcatgccat     3600

accgcgaaag gttttgcgcc attcgatggt gtccgggatc tcgacgctct cccttatgcg     3660

actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa     3720

ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca     3780

tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg     3840

tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga     3900

tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa ttaatacgac tcactatagg     3960

ggaattgtga gcggataaca attcccctca agaaataatt ttgtttaact ttaagaagga     4020

gatatacata tgggatccat ggagttcgtt aacaaacagt tcaactataa agacccagtt     4080

aacggtgttg acattgctta catcaaaatc ccgaacgctg gccagatgca gccggtaaag     4140

gcattcaaaa tccacaacaa aatctgggtt atcccggaac gtgatacctt tactaacccg     4200

gaagaaggtg acctgaaccc gccaccggaa gcgaaacagg tgccggtatc ttactatgac     4260

tccacctacc tgtctaccga taacgaaaag gacaactacc tgaaaggtgt tactaaactg     4320

ttcgagcgta tttactccac cgacctgggc cgtatgctgc tgactagcat cgttcgcggt     4380

atcccgttct ggggcggttc taccatcgat accgaactga aagtaatcga cactaactgc     4440

atcaacgtta ttcagccgga cggttcctat cgttccgaag aactgaacct ggtgatcatc     4500

ggcccgtctg ctgatatcat ccagttcgag tgtaagagct ttggtcacga agttctgaac     4560

ctcacccgta acggctacgg ttccactcag tacatccgtt tctctccgga cttcaccttc     4620

ggttttgaag aatccctgga agtagacacg aacccactgc tgggcgctgg taaattcgca     4680

actgatcctg cggttaccct ggctcacgaa ctgattcatg caggccaccg cctgtacggt     4740

atcgccatca atccgaaccg tgtcttcaaa gttaacacca acgcgtatta cgagatgtcc     4800

ggtctggaag ttagcttcga agaactgcgt acttttggcg gtcacgacgc taaattcatc     4860

gactctctgc aagaaaacga gttccgtctg tactactata acaagttcaa agatatcgca     4920

tccaccctga acaaagcgaa atccatcgtg ggtaccactg cttctctcca gtacatgaag     4980

aacgttttta aagaaaaata cctgctcagc gaagacacct ccggcaaatt ctctgtagac     5040

aagttgaaat tcgataaact ttacaaaatg ctgactgaaa tttacaccga agacaacttc     5100

gttaagttct ttaaagttct gaaccgcaaa acctatctga acttcgacaa ggcagtattc     5160

aaaatcaaca tcgtgccgaa agttaactac actatctacg atggtttcaa cctgcgtaac     5220

accaacctgg ctgctaattt taacggccag aacacggaaa tcaacaacat gaacttcaca     5280

aaactgaaaa acttcactgg tctgttcgag ttttacaagc tgctgtgcgt cgacggcatc     5340

attacctcca aaactaaatc tctgatagaa ggtagaaaca aagcgctgaa cctgcagtgt     5400

atcaaggtta acaactggga tttattcttc agcccgagtg aagacaactt caccaacgac     5460

ctgaacaaag gtgaagaaat cacctcagat actaacatcg aagcagccga agaaaacatc     5520

tcgctggacc tgatccagca gtactacctg acctttaatt tcgacaacga gccggaaaac     5580

atttctatcg aaaacctgag ctctgatatc atcggccagc tggaactgat gccgaacatc     5640

gaacgtttcc caaacggtaa aaagtacgag ctggacaaat ataccatgtt ccactacctg     5700

cgcgcgcagg aatttgaaca cggcaaatcc cgtatcgcac tgactaactc cgttaacgaa     5760

gctctgctca acccgtcccg tgtatacacc ttcttctcta gcgactacgt gaaaaaggtc     5820

aacaaagcga ctgaagctgc aatgttcttg ggttgggttg aacagcttgt ttatgatttt     5880

accgacgaga cgtccgaagt atctactacc gacaaaattg cggatatcac tatcatcatc     5940

ccgtacatcg gtccggctct gaacattggc aacatgctgt acaaagacga cttcgttggc     6000

gcactgatct tctccggtgc ggtgatcctg ctggagttca tcccggaaat cgccatcccg     6060

gtactgggca cctttgctct ggtttcttac attgcaaaca aggttctgac tgtacaaacc     6120

atcgacaacg cgctgagcaa acgtaacgaa aaatgggatg aagtttacaa atatatcgtg     6180

accaactggc tggctaaggt taatactcag atcgacctca tccgcaaaaa aatgaaagaa     6240

gcactggaaa accaggcgga agctaccaag gcaatcatta actaccagta caaccagtac     6300

accgaggaag aaaaaaacaa catcaacttc aacatcgacg atctgtcctc taaactgaac     6360

gaatccatca acaaagctat gatcaacatc aacaagttcc tgaaccagtg ctctgtaagc     6420

tatctgatga actccatgat cccgtacggt gttaaacgtc tggaggactt cgatgcgtct     6480

ctgaaagacg ccctgctgaa atacatttac gacaaccgtg gcactctgat cggtcaggtt     6540

gatcgtctga aggacaaagt gaacaatacc ttatcgaccg acatcccttt tcagctcagt     6600

aaatatgtcg ataaccaacg ccttttgtcc actctagaag gcggtggcgg tagcggtggc     6660

ggtggcagcg gcggtggcgg tagcgcacta gacaacagcg accctaaatg cccactgagt     6720

catgaaggat actgccttaa tgatggtgtt tgtatgtaca taggaacatt ggaccgttat     6780

gcttgcaatt gtgtagtggg ctatgtcggg gaaaggtgtc aatatcgaga tctcaagctg     6840

gcagagttaa gagggctaga agcacaccat catcaccacc atcaccatca ccattaatga     6900

aagcttgcgg ccgcactcga gcaccaccac caccaccact gagatccggc tgctaacaaa     6960

gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt     7020

ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggat       7078


<210>  6
<211>  952
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Polypeptide sequence of EGF-liganded polypeptide

<400>  6

Met Glu Phe Val Asn Lys Gln Phe Asn Tyr Lys Asp Pro Val Asn Gly 
1               5                   10                  15      


Val Asp Ile Ala Tyr Ile Lys Ile Pro Asn Ala Gly Gln Met Gln Pro 
            20                  25                  30          


Val Lys Ala Phe Lys Ile His Asn Lys Ile Trp Val Ile Pro Glu Arg 
        35                  40                  45              


Asp Thr Phe Thr Asn Pro Glu Glu Gly Asp Leu Asn Pro Pro Pro Glu 
    50                  55                  60                  


Ala Lys Gln Val Pro Val Ser Tyr Tyr Asp Ser Thr Tyr Leu Ser Thr 
65                  70                  75                  80  


Asp Asn Glu Lys Asp Asn Tyr Leu Lys Gly Val Thr Lys Leu Phe Glu 
                85                  90                  95      


Arg Ile Tyr Ser Thr Asp Leu Gly Arg Met Leu Leu Thr Ser Ile Val 
            100                 105                 110         


Arg Gly Ile Pro Phe Trp Gly Gly Ser Thr Ile Asp Thr Glu Leu Lys 
        115                 120                 125             


Val Ile Asp Thr Asn Cys Ile Asn Val Ile Gln Pro Asp Gly Ser Tyr 
    130                 135                 140                 


Arg Ser Glu Glu Leu Asn Leu Val Ile Ile Gly Pro Ser Ala Asp Ile 
145                 150                 155                 160 


Ile Gln Phe Glu Cys Lys Ser Phe Gly His Glu Val Leu Asn Leu Thr 
                165                 170                 175     


Arg Asn Gly Tyr Gly Ser Thr Gln Tyr Ile Arg Phe Ser Pro Asp Phe 
            180                 185                 190         


Thr Phe Gly Phe Glu Glu Ser Leu Glu Val Asp Thr Asn Pro Leu Leu 
        195                 200                 205             


Gly Ala Gly Lys Phe Ala Thr Asp Pro Ala Val Thr Leu Ala His Glu 
    210                 215                 220                 


Leu Ile His Ala Gly His Arg Leu Tyr Gly Ile Ala Ile Asn Pro Asn 
225                 230                 235                 240 


Arg Val Phe Lys Val Asn Thr Asn Ala Tyr Tyr Glu Met Ser Gly Leu 
                245                 250                 255     


Glu Val Ser Phe Glu Glu Leu Arg Thr Phe Gly Gly His Asp Ala Lys 
            260                 265                 270         


Phe Ile Asp Ser Leu Gln Glu Asn Glu Phe Arg Leu Tyr Tyr Tyr Asn 
        275                 280                 285             


Lys Phe Lys Asp Ile Ala Ser Thr Leu Asn Lys Ala Lys Ser Ile Val 
    290                 295                 300                 


Gly Thr Thr Ala Ser Leu Gln Tyr Met Lys Asn Val Phe Lys Glu Lys 
305                 310                 315                 320 


Tyr Leu Leu Ser Glu Asp Thr Ser Gly Lys Phe Ser Val Asp Lys Leu 
                325                 330                 335     


Lys Phe Asp Lys Leu Tyr Lys Met Leu Thr Glu Ile Tyr Thr Glu Asp 
            340                 345                 350         


Asn Phe Val Lys Phe Phe Lys Val Leu Asn Arg Lys Thr Tyr Leu Asn 
        355                 360                 365             


Phe Asp Lys Ala Val Phe Lys Ile Asn Ile Val Pro Lys Val Asn Tyr 
    370                 375                 380                 


Thr Ile Tyr Asp Gly Phe Asn Leu Arg Asn Thr Asn Leu Ala Ala Asn 
385                 390                 395                 400 


Phe Asn Gly Gln Asn Thr Glu Ile Asn Asn Met Asn Phe Thr Lys Leu 
                405                 410                 415     


Lys Asn Phe Thr Gly Leu Phe Glu Phe Tyr Lys Leu Leu Cys Val Asp 
            420                 425                 430         


Gly Ile Ile Thr Ser Lys Thr Lys Ser Leu Ile Glu Gly Arg Asn Lys 
        435                 440                 445             


Ala Leu Asn Leu Gln Cys Ile Lys Val Asn Asn Trp Asp Leu Phe Phe 
    450                 455                 460                 


Ser Pro Ser Glu Asp Asn Phe Thr Asn Asp Leu Asn Lys Gly Glu Glu 
465                 470                 475                 480 


Ile Thr Ser Asp Thr Asn Ile Glu Ala Ala Glu Glu Asn Ile Ser Leu 
                485                 490                 495     


Asp Leu Ile Gln Gln Tyr Tyr Leu Thr Phe Asn Phe Asp Asn Glu Pro 
            500                 505                 510         


Glu Asn Ile Ser Ile Glu Asn Leu Ser Ser Asp Ile Ile Gly Gln Leu 
        515                 520                 525             


Glu Leu Met Pro Asn Ile Glu Arg Phe Pro Asn Gly Lys Lys Tyr Glu 
    530                 535                 540                 


Leu Asp Lys Tyr Thr Met Phe His Tyr Leu Arg Ala Gln Glu Phe Glu 
545                 550                 555                 560 


His Gly Lys Ser Arg Ile Ala Leu Thr Asn Ser Val Asn Glu Ala Leu 
                565                 570                 575     


Leu Asn Pro Ser Arg Val Tyr Thr Phe Phe Ser Ser Asp Tyr Val Lys 
            580                 585                 590         


Lys Val Asn Lys Ala Thr Glu Ala Ala Met Phe Leu Gly Trp Val Glu 
        595                 600                 605             


Gln Leu Val Tyr Asp Phe Thr Asp Glu Thr Ser Glu Val Ser Thr Thr 
    610                 615                 620                 


Asp Lys Ile Ala Asp Ile Thr Ile Ile Ile Pro Tyr Ile Gly Pro Ala 
625                 630                 635                 640 


Leu Asn Ile Gly Asn Met Leu Tyr Lys Asp Asp Phe Val Gly Ala Leu 
                645                 650                 655     


Ile Phe Ser Gly Ala Val Ile Leu Leu Glu Phe Ile Pro Glu Ile Ala 
            660                 665                 670         


Ile Pro Val Leu Gly Thr Phe Ala Leu Val Ser Tyr Ile Ala Asn Lys 
        675                 680                 685             


Val Leu Thr Val Gln Thr Ile Asp Asn Ala Leu Ser Lys Arg Asn Glu 
    690                 695                 700                 


Lys Trp Asp Glu Val Tyr Lys Tyr Ile Val Thr Asn Trp Leu Ala Lys 
705                 710                 715                 720 


Val Asn Thr Gln Ile Asp Leu Ile Arg Lys Lys Met Lys Glu Ala Leu 
                725                 730                 735     


Glu Asn Gln Ala Glu Ala Thr Lys Ala Ile Ile Asn Tyr Gln Tyr Asn 
            740                 745                 750         


Gln Tyr Thr Glu Glu Glu Lys Asn Asn Ile Asn Phe Asn Ile Asp Asp 
        755                 760                 765             


Leu Ser Ser Lys Leu Asn Glu Ser Ile Asn Lys Ala Met Ile Asn Ile 
    770                 775                 780                 


Asn Lys Phe Leu Asn Gln Cys Ser Val Ser Tyr Leu Met Asn Ser Met 
785                 790                 795                 800 


Ile Pro Tyr Gly Val Lys Arg Leu Glu Asp Phe Asp Ala Ser Leu Lys 
                805                 810                 815     


Asp Ala Leu Leu Lys Tyr Ile Tyr Asp Asn Arg Gly Thr Leu Ile Gly 
            820                 825                 830         


Gln Val Asp Arg Leu Lys Asp Lys Val Asn Asn Thr Leu Ser Thr Asp 
        835                 840                 845             


Ile Pro Phe Gln Leu Ser Lys Tyr Val Asp Asn Gln Arg Leu Leu Ser 
    850                 855                 860                 


Thr Leu Glu Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
865                 870                 875                 880 


Gly Ser Ala Leu Asp Asn Ser Asp Pro Lys Cys Pro Leu Ser His Glu 
                885                 890                 895     


Gly Tyr Cys Leu Asn Asp Gly Val Cys Met Tyr Ile Gly Thr Leu Asp 
            900                 905                 910         


Arg Tyr Ala Cys Asn Cys Val Val Gly Tyr Val Gly Glu Arg Cys Gln 
        915                 920                 925             


Tyr Arg Asp Leu Lys Leu Ala Glu Leu Arg Gly Leu Glu Ala His His 
    930                 935                 940                 


His His His His His His His His 
945                 950         


<210>  7
<211>  6937
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of nociceptin-liganded polypeptide

<400>  7
tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg       60

cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc      120

ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg      180

gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc      240

acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt      300

ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc      360

ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta      420

acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt      480

cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat      540

ccgctcatga attaattctt agaaaaactc atcgagcatc aaatgaaact gcaatttatt      600

catatcagga ttatcaatac catatttttg aaaaagccgt ttctgtaatg aaggagaaaa      660

ctcaccgagg cagttccata ggatggcaag atcctggtat cggtctgcga ttccgactcg      720

tccaacatca atacaaccta ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa      780

atcaccatga gtgacgactg aatccggtga gaatggcaaa agtttatgca tttctttcca      840

gacttgttca acaggccagc cattacgctc gtcatcaaaa tcactcgcat caaccaaacc      900

gttattcatt cgtgattgcg cctgagcgag acgaaatacg cgatcgctgt taaaaggaca      960

attacaaaca ggaatcgaat gcaaccggcg caggaacact gccagcgcat caacaatatt     1020

ttcacctgaa tcaggatatt cttctaatac ctggaatgct gttttcccgg ggatcgcagt     1080

ggtgagtaac catgcatcat caggagtacg gataaaatgc ttgatggtcg gaagaggcat     1140

aaattccgtc agccagttta gtctgaccat ctcatctgta acatcattgg caacgctacc     1200

tttgccatgt ttcagaaaca actctggcgc atcgggcttc ccatacaatc gatagattgt     1260

cgcacctgat tgcccgacat tatcgcgagc ccatttatac ccatataaat cagcatccat     1320

gttggaattt aatcgcggcc tagagcaaga cgtttcccgt tgaatatggc tcataacacc     1380

ccttgtatta ctgtttatgt aagcagacag ttttattgtt catgaccaaa atcccttaac     1440

gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag     1500

atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg     1560

tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca     1620

gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga     1680

actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca     1740

gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc     1800

agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca     1860

ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa     1920

aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc     1980

cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc     2040

gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg     2100

cctttttacg gttcctggcc ttttgctggc cttttgctca catcggcgat aatggcctgc     2160

ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag     2220

attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg     2280

ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca     2340

gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg     2400

aaggctctca agggcatcgg tcgagatccc ggtgcctaat gagtgagcta acttacatta     2460

attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa     2520

tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgccaggg tggtttttct     2580

tttcaccagt gagacgggca acagctgatt gcccttcacc gcctggccct gagagagttg     2640

cagcaagcgg tccacgctgg tttgccccag caggcgaaaa tcctgtttga tggtggttaa     2700

cggcgggata taacatgagc tgtcttcggt atcgtcgtat cccactaccg agatatccgc     2760

accaacgcgc agcccggact cggtaatggc gcgcattgcg cccagcgcca tctgatcgtt     2820

ggcaaccagc atcgcagtgg gaacgatgcc ctcattcagc atttgcatgg tttgttgaaa     2880

accggacatg gcactccagt cgccttcccg ttccgctatc ggctgaattt gattgcgagt     2940

gagatattta tgccagccag ccagacgcag acgcgccgag acagaactta atgggcccgc     3000

taacagcgcg atttgctggt gacccaatgc gaccagatgc tccacgccca gtcgcgtacc     3060

gtcttcatgg gagaaaataa tactgttgat gggtgtctgg tcagagacat caagaaataa     3120

cgccggaaca ttagtgcagg cagcttccac agcaatggca tcctggtcat ccagcggata     3180

gttaatgatc agcccactga cgcgttgcgc gagaagattg tgcaccgccg ctttacaggc     3240

ttcgacgccg cttcgttcta ccatcgacac caccacgctg gcacccagtt gatcggcgcg     3300

agatttaatc gccgcgacaa tttgcgacgg cgcgtgcagg gccagactgg aggtggcaac     3360

gccaatcagc aacgactgtt tgcccgccag ttgttgtgcc acgcggttgg gaatgtaatt     3420

cagctccgcc atcgccgctt ccactttttc ccgcgttttc gcagaaacgt ggctggcctg     3480

gttcaccacg cgggaaacgg tctgataaga gacaccggca tactctgcga catcgtataa     3540

cgttactggt ttcacattca ccaccctgaa ttgactctct tccgggcgct atcatgccat     3600

accgcgaaag gttttgcgcc attcgatggt gtccgggatc tcgacgctct cccttatgcg     3660

actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa     3720

ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca     3780

tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg     3840

tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga     3900

tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa ttaatacgac tcactatagg     3960

ggaattgtga gcggataaca attcccctca agaaataatt ttgtttaact ttaagaagga     4020

gatatacata tgggcagcat ggaatttgtg aacaaacagt tcaactataa ggatccggtt     4080

aatggtgtgg atatcgccta tatcaaaatt ccgaatgcag gtcagatgca gccggttaaa     4140

gcctttaaaa tccataacaa aatttgggtg attccggaac gtgatacctt taccaatccg     4200

gaagaaggtg atctgaatcc gcctccggaa gcaaaacagg ttccggttag ctattatgat     4260

agcacctatc tgagcaccga taacgagaaa gataactatc tgaaaggtgt gaccaaactg     4320

tttgaacgca tttatagtac cgatctgggt cgtatgctgc tgaccagcat tgttcgtggt     4380

attccgtttt ggggtggtag caccattgat accgaactga aagttattga caccaactgc     4440

attaatgtga ttcagccgga tggtagctat cgtagcgaag aactgaatct ggttattatt     4500

ggtccgagcg cagatatcat tcagtttgaa tgtaaatcct ttggccacga agttctgaat     4560

ctgacccgta atggttatgg tagtacccag tatattcgtt tcagtccgga ttttaccttt     4620

ggctttgaag aaagcctgga agttgataca aatccgctgt taggtgcagg taaatttgca     4680

accgatccgg cagttaccct ggcacatgaa ctgattcatg ccggtcatcg tctgtatggt     4740

attgcaatta atccgaaccg tgtgttcaaa gtgaatacca acgcatatta tgaaatgagc     4800

ggtctggaag tgtcatttga agaactgcgt acctttggtg gtcatgatgc caaatttatc     4860

gatagcctgc aagaaaatga atttcgcctg tactactata acaaattcaa ggatattgcg     4920

agcaccctga ataaagccaa aagcattgtt ggcaccaccg caagcctgca gtatatgaaa     4980

aatgtgttta aagaaaaata tctgctgagc gaagatacca gcggtaaatt tagcgttgac     5040

aaactgaaat tcgataaact gtacaagatg ctgaccgaga tttataccga agataacttc     5100

gtgaagtttt tcaaagtgct gaaccgcaaa acctacctga actttgataa agccgtgttc     5160

aaaatcaaca tcgtgccgaa agtgaactat accatctatg atggttttaa cctgcgcaat     5220

accaatctgg cagcaaactt taatggtcag aacaccgaaa tcaacaacat gaactttacc     5280

aaactgaaga acttcaccgg tctgttcgaa ttttacaaac tgctgtgtgt ggatggcatt     5340

attaccagca aaaccaaatc cgatgatgac gataaattcg gtggttttac cggtgcacgt     5400

aaaagcgcac gtaaacgtaa aaatcaggca ctggcaggcg gtggtggtag cggtggcggt     5460

ggttcaggtg gtggtggctc agcactggtt ctgcagtgta ttaaagttaa taactgggac     5520

ctgtttttta gcccgagcga ggataatttc accaacgatc tgaacaaagg cgaagaaatt     5580

accagcgata ccaatattga agcagccgaa gaaaacatta gcctggatct gattcagcag     5640

tattatctga ccttcaactt cgataatgag ccggaaaata tcagcattga aaacctgagc     5700

agcgatatta ttggccagct ggaactgatg ccgaatattg aacgttttcc gaacggcaaa     5760

aaatacgagc tggataaata caccatgttc cattatctgc gtgcccaaga atttgaacat     5820

ggtaaaagcc gtattgcact gaccaatagc gttaatgaag cactgctgaa cccgagccgt     5880

gtttatacct tttttagcag cgattacgtg aaaaaggtta acaaagcaac cgaagcagcc     5940

atgtttttag gttgggttga acagctggtt tatgatttca ccgatgaaac cagcgaagtt     6000

agcaccaccg ataaaattgc agatattacc atcatcatcc cgtatatcgg tccggcactg     6060

aatattggca atatgctgta taaagacgat tttgtgggtg ccctgatctt tagcggtgca     6120

gttattctgc tggaatttat tccggaaatt gccattccgg ttctgggcac ctttgcactg     6180

gtgagctata ttgcaaataa agttctgacc gtgcagacca tcgataatgc actgagcaaa     6240

cgtaacgaaa aatgggatga agtgtacaag tatatcgtga ccaattggct ggcaaaagtt     6300

aacacccaga ttgacctgat tcgcaagaag atgaaagaag cactggaaaa ccaggcagaa     6360

gcaaccaaag ccattattaa ctatcagtac aaccagtaca ccgaagaaga gaagaataac     6420

atcaacttca acatcgatga tctgagcagc aagctgaatg aaagcatcaa caaagccatg     6480

atcaacatta acaaatttct gaatcagtgc agcgtgagct atctgatgaa tagcatgatt     6540

ccgtatggtg tgaaacgtct ggaagatttt gatgcaagcc tgaaagatgc cctgctgaaa     6600

tatatctatg ataatcgtgg caccctgatt ggtcaggttg atcgtctgaa agataaagtg     6660

aacaacaccc tgagtaccga tattcctttt cagctgagca aatatgtgga taatcagcgt     6720

ctgctgagta ccctggatca tcatcaccat caccactaaa agcttgcggc cgcactcgag     6780

caccaccacc accaccactg agatccggct gctaacaaag cccgaaagga agctgagttg     6840

gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg     6900

aggggttttt tgctgaaagg aggaactata tccggat                              6937


<210>  8
<211>  909
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Polypeptide sequence of nociceptin-liganded polypeptide

<400>  8

Met Gly Ser Met Glu Phe Val Asn Lys Gln Phe Asn Tyr Lys Asp Pro 
1               5                   10                  15      


Val Asn Gly Val Asp Ile Ala Tyr Ile Lys Ile Pro Asn Ala Gly Gln 
            20                  25                  30          


Met Gln Pro Val Lys Ala Phe Lys Ile His Asn Lys Ile Trp Val Ile 
        35                  40                  45              


Pro Glu Arg Asp Thr Phe Thr Asn Pro Glu Glu Gly Asp Leu Asn Pro 
    50                  55                  60                  


Pro Pro Glu Ala Lys Gln Val Pro Val Ser Tyr Tyr Asp Ser Thr Tyr 
65                  70                  75                  80  


Leu Ser Thr Asp Asn Glu Lys Asp Asn Tyr Leu Lys Gly Val Thr Lys 
                85                  90                  95      


Leu Phe Glu Arg Ile Tyr Ser Thr Asp Leu Gly Arg Met Leu Leu Thr 
            100                 105                 110         


Ser Ile Val Arg Gly Ile Pro Phe Trp Gly Gly Ser Thr Ile Asp Thr 
        115                 120                 125             


Glu Leu Lys Val Ile Asp Thr Asn Cys Ile Asn Val Ile Gln Pro Asp 
    130                 135                 140                 


Gly Ser Tyr Arg Ser Glu Glu Leu Asn Leu Val Ile Ile Gly Pro Ser 
145                 150                 155                 160 


Ala Asp Ile Ile Gln Phe Glu Cys Lys Ser Phe Gly His Glu Val Leu 
                165                 170                 175     


Asn Leu Thr Arg Asn Gly Tyr Gly Ser Thr Gln Tyr Ile Arg Phe Ser 
            180                 185                 190         


Pro Asp Phe Thr Phe Gly Phe Glu Glu Ser Leu Glu Val Asp Thr Asn 
        195                 200                 205             


Pro Leu Leu Gly Ala Gly Lys Phe Ala Thr Asp Pro Ala Val Thr Leu 
    210                 215                 220                 


Ala His Glu Leu Ile His Ala Gly His Arg Leu Tyr Gly Ile Ala Ile 
225                 230                 235                 240 


Asn Pro Asn Arg Val Phe Lys Val Asn Thr Asn Ala Tyr Tyr Glu Met 
                245                 250                 255     


Ser Gly Leu Glu Val Ser Phe Glu Glu Leu Arg Thr Phe Gly Gly His 
            260                 265                 270         


Asp Ala Lys Phe Ile Asp Ser Leu Gln Glu Asn Glu Phe Arg Leu Tyr 
        275                 280                 285             


Tyr Tyr Asn Lys Phe Lys Asp Ile Ala Ser Thr Leu Asn Lys Ala Lys 
    290                 295                 300                 


Ser Ile Val Gly Thr Thr Ala Ser Leu Gln Tyr Met Lys Asn Val Phe 
305                 310                 315                 320 


Lys Glu Lys Tyr Leu Leu Ser Glu Asp Thr Ser Gly Lys Phe Ser Val 
                325                 330                 335     


Asp Lys Leu Lys Phe Asp Lys Leu Tyr Lys Met Leu Thr Glu Ile Tyr 
            340                 345                 350         


Thr Glu Asp Asn Phe Val Lys Phe Phe Lys Val Leu Asn Arg Lys Thr 
        355                 360                 365             


Tyr Leu Asn Phe Asp Lys Ala Val Phe Lys Ile Asn Ile Val Pro Lys 
    370                 375                 380                 


Val Asn Tyr Thr Ile Tyr Asp Gly Phe Asn Leu Arg Asn Thr Asn Leu 
385                 390                 395                 400 


Ala Ala Asn Phe Asn Gly Gln Asn Thr Glu Ile Asn Asn Met Asn Phe 
                405                 410                 415     


Thr Lys Leu Lys Asn Phe Thr Gly Leu Phe Glu Phe Tyr Lys Leu Leu 
            420                 425                 430         


Cys Val Asp Gly Ile Ile Thr Ser Lys Thr Lys Ser Asp Asp Asp Asp 
        435                 440                 445             


Lys Phe Gly Gly Phe Thr Gly Ala Arg Lys Ser Ala Arg Lys Arg Lys 
    450                 455                 460                 


Asn Gln Ala Leu Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
465                 470                 475                 480 


Gly Gly Gly Ser Ala Leu Val Leu Gln Cys Ile Lys Val Asn Asn Trp 
                485                 490                 495     


Asp Leu Phe Phe Ser Pro Ser Glu Asp Asn Phe Thr Asn Asp Leu Asn 
            500                 505                 510         


Lys Gly Glu Glu Ile Thr Ser Asp Thr Asn Ile Glu Ala Ala Glu Glu 
        515                 520                 525             


Asn Ile Ser Leu Asp Leu Ile Gln Gln Tyr Tyr Leu Thr Phe Asn Phe 
    530                 535                 540                 


Asp Asn Glu Pro Glu Asn Ile Ser Ile Glu Asn Leu Ser Ser Asp Ile 
545                 550                 555                 560 


Ile Gly Gln Leu Glu Leu Met Pro Asn Ile Glu Arg Phe Pro Asn Gly 
                565                 570                 575     


Lys Lys Tyr Glu Leu Asp Lys Tyr Thr Met Phe His Tyr Leu Arg Ala 
            580                 585                 590         


Gln Glu Phe Glu His Gly Lys Ser Arg Ile Ala Leu Thr Asn Ser Val 
        595                 600                 605             


Asn Glu Ala Leu Leu Asn Pro Ser Arg Val Tyr Thr Phe Phe Ser Ser 
    610                 615                 620                 


Asp Tyr Val Lys Lys Val Asn Lys Ala Thr Glu Ala Ala Met Phe Leu 
625                 630                 635                 640 


Gly Trp Val Glu Gln Leu Val Tyr Asp Phe Thr Asp Glu Thr Ser Glu 
                645                 650                 655     


Val Ser Thr Thr Asp Lys Ile Ala Asp Ile Thr Ile Ile Ile Pro Tyr 
            660                 665                 670         


Ile Gly Pro Ala Leu Asn Ile Gly Asn Met Leu Tyr Lys Asp Asp Phe 
        675                 680                 685             


Val Gly Ala Leu Ile Phe Ser Gly Ala Val Ile Leu Leu Glu Phe Ile 
    690                 695                 700                 


Pro Glu Ile Ala Ile Pro Val Leu Gly Thr Phe Ala Leu Val Ser Tyr 
705                 710                 715                 720 


Ile Ala Asn Lys Val Leu Thr Val Gln Thr Ile Asp Asn Ala Leu Ser 
                725                 730                 735     


Lys Arg Asn Glu Lys Trp Asp Glu Val Tyr Lys Tyr Ile Val Thr Asn 
            740                 745                 750         


Trp Leu Ala Lys Val Asn Thr Gln Ile Asp Leu Ile Arg Lys Lys Met 
        755                 760                 765             


Lys Glu Ala Leu Glu Asn Gln Ala Glu Ala Thr Lys Ala Ile Ile Asn 
    770                 775                 780                 


Tyr Gln Tyr Asn Gln Tyr Thr Glu Glu Glu Lys Asn Asn Ile Asn Phe 
785                 790                 795                 800 


Asn Ile Asp Asp Leu Ser Ser Lys Leu Asn Glu Ser Ile Asn Lys Ala 
                805                 810                 815     


Met Ile Asn Ile Asn Lys Phe Leu Asn Gln Cys Ser Val Ser Tyr Leu 
            820                 825                 830         


Met Asn Ser Met Ile Pro Tyr Gly Val Lys Arg Leu Glu Asp Phe Asp 
        835                 840                 845             


Ala Ser Leu Lys Asp Ala Leu Leu Lys Tyr Ile Tyr Asp Asn Arg Gly 
    850                 855                 860                 


Thr Leu Ile Gly Gln Val Asp Arg Leu Lys Asp Lys Val Asn Asn Thr 
865                 870                 875                 880 


Leu Ser Thr Asp Ile Pro Phe Gln Leu Ser Lys Tyr Val Asp Asn Gln 
                885                 890                 895     


Arg Leu Leu Ser Thr Leu Asp His His His His His His 
            900                 905                 


<210>  9
<211>  7822
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of EGF-liganded polypeptide GFP-tagged

<400>  9
tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg       60

cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc      120

ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg      180

gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc      240

acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt      300

ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc      360

ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta      420

acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt      480

cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat      540

ccgctcatga attaattctt agaaaaactc atcgagcatc aaatgaaact gcaatttatt      600

catatcagga ttatcaatac catatttttg aaaaagccgt ttctgtaatg aaggagaaaa      660

ctcaccgagg cagttccata ggatggcaag atcctggtat cggtctgcga ttccgactcg      720

tccaacatca atacaaccta ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa      780

atcaccatga gtgacgactg aatccggtga gaatggcaaa agtttatgca tttctttcca      840

gacttgttca acaggccagc cattacgctc gtcatcaaaa tcactcgcat caaccaaacc      900

gttattcatt cgtgattgcg cctgagcgag acgaaatacg cgatcgctgt taaaaggaca      960

attacaaaca ggaatcgaat gcaaccggcg caggaacact gccagcgcat caacaatatt     1020

ttcacctgaa tcaggatatt cttctaatac ctggaatgct gttttcccgg ggatcgcagt     1080

ggtgagtaac catgcatcat caggagtacg gataaaatgc ttgatggtcg gaagaggcat     1140

aaattccgtc agccagttta gtctgaccat ctcatctgta acatcattgg caacgctacc     1200

tttgccatgt ttcagaaaca actctggcgc atcgggcttc ccatacaatc gatagattgt     1260

cgcacctgat tgcccgacat tatcgcgagc ccatttatac ccatataaat cagcatccat     1320

gttggaattt aatcgcggcc tagagcaaga cgtttcccgt tgaatatggc tcataacacc     1380

ccttgtatta ctgtttatgt aagcagacag ttttattgtt catgaccaaa atcccttaac     1440

gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag     1500

atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg     1560

tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca     1620

gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga     1680

actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca     1740

gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc     1800

agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca     1860

ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa     1920

aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc     1980

cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc     2040

gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg     2100

cctttttacg gttcctggcc ttttgctggc cttttgctca catcggcgat aatggcctgc     2160

ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag     2220

attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg     2280

ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca     2340

gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg     2400

aaggctctca agggcatcgg tcgagatccc ggtgcctaat gagtgagcta acttacatta     2460

attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa     2520

tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgccaggg tggtttttct     2580

tttcaccagt gagacgggca acagctgatt gcccttcacc gcctggccct gagagagttg     2640

cagcaagcgg tccacgctgg tttgccccag caggcgaaaa tcctgtttga tggtggttaa     2700

cggcgggata taacatgagc tgtcttcggt atcgtcgtat cccactaccg agatatccgc     2760

accaacgcgc agcccggact cggtaatggc gcgcattgcg cccagcgcca tctgatcgtt     2820

ggcaaccagc atcgcagtgg gaacgatgcc ctcattcagc atttgcatgg tttgttgaaa     2880

accggacatg gcactccagt cgccttcccg ttccgctatc ggctgaattt gattgcgagt     2940

gagatattta tgccagccag ccagacgcag acgcgccgag acagaactta atgggcccgc     3000

taacagcgcg atttgctggt gacccaatgc gaccagatgc tccacgccca gtcgcgtacc     3060

gtcttcatgg gagaaaataa tactgttgat gggtgtctgg tcagagacat caagaaataa     3120

cgccggaaca ttagtgcagg cagcttccac agcaatggca tcctggtcat ccagcggata     3180

gttaatgatc agcccactga cgcgttgcgc gagaagattg tgcaccgccg ctttacaggc     3240

ttcgacgccg cttcgttcta ccatcgacac caccacgctg gcacccagtt gatcggcgcg     3300

agatttaatc gccgcgacaa tttgcgacgg cgcgtgcagg gccagactgg aggtggcaac     3360

gccaatcagc aacgactgtt tgcccgccag ttgttgtgcc acgcggttgg gaatgtaatt     3420

cagctccgcc atcgccgctt ccactttttc ccgcgttttc gcagaaacgt ggctggcctg     3480

gttcaccacg cgggaaacgg tctgataaga gacaccggca tactctgcga catcgtataa     3540

cgttactggt ttcacattca ccaccctgaa ttgactctct tccgggcgct atcatgccat     3600

accgcgaaag gttttgcgcc attcgatggt gtccgggatc tcgacgctct cccttatgcg     3660

actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa     3720

ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca     3780

tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg     3840

tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga     3900

tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa ttaatacgac tcactatagg     3960

ggaattgtga gcggataaca attcccctca agaaataatt ttgtttaact ttaagaagga     4020

gatatacata tgatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg     4080

gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc     4140

gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg     4200

ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc     4260

gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag     4320

cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag     4380

ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac     4440

atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac     4500

aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc     4560

gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg     4620

cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc     4680

gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactcacgg catggacgag     4740

ctgtacaagg gcggcagcgg cggcggcagc ggcggcggat ccatggagtt cgttaacaaa     4800

cagttcaact ataaagaccc agttaacggt gttgacattg cttacatcaa aatcccgaac     4860

gctggccaga tgcagccggt aaaggcattc aaaatccaca acaaaatctg ggttatcccg     4920

gaacgtgata cctttactaa cccggaagaa ggtgacctga acccgccacc ggaagcgaaa     4980

caggtgccgg tatcttacta tgactccacc tacctgtcta ccgataacga aaaggacaac     5040

tacctgaaag gtgttactaa actgttcgag cgtatttact ccaccgacct gggccgtatg     5100

ctgctgacta gcatcgttcg cggtatcccg ttctggggcg gttctaccat cgataccgaa     5160

ctgaaagtaa tcgacactaa ctgcatcaac gttattcagc cggacggttc ctatcgttcc     5220

gaagaactga acctggtgat catcggcccg tctgctgata tcatccagtt cgagtgtaag     5280

agctttggtc acgaagttct gaacctcacc cgtaacggct acggttccac tcagtacatc     5340

cgtttctctc cggacttcac cttcggtttt gaagaatccc tggaagtaga cacgaaccca     5400

ctgctgggcg ctggtaaatt cgcaactgat cctgcggtta ccctggctca cgaactgatt     5460

catgcaggcc accgcctgta cggtatcgcc atcaatccga accgtgtctt caaagttaac     5520

accaacgcgt attacgagat gtccggtctg gaagttagct tcgaagaact gcgtactttt     5580

ggcggtcacg acgctaaatt catcgactct ctgcaagaaa acgagttccg tctgtactac     5640

tataacaagt tcaaagatat cgcatccacc ctgaacaaag cgaaatccat cgtgggtacc     5700

actgcttctc tccagtacat gaagaacgtt tttaaagaaa aatacctgct cagcgaagac     5760

acctccggca aattctctgt agacaagttg aaattcgata aactttacaa aatgctgact     5820

gaaatttaca ccgaagacaa cttcgttaag ttctttaaag ttctgaaccg caaaacctat     5880

ctgaacttcg acaaggcagt attcaaaatc aacatcgtgc cgaaagttaa ctacactatc     5940

tacgatggtt tcaacctgcg taacaccaac ctggctgcta attttaacgg ccagaacacg     6000

gaaatcaaca acatgaactt cacaaaactg aaaaacttca ctggtctgtt cgagttttac     6060

aagctgctgt gcgtcgacgg catcattacc tccaaaacta aatctctgat agaaggtaga     6120

aacaaagcgc tgaacctgca gtgtatcaag gttaacaact gggatttatt cttcagcccg     6180

agtgaagaca acttcaccaa cgacctgaac aaaggtgaag aaatcacctc agatactaac     6240

atcgaagcag ccgaagaaaa catctcgctg gacctgatcc agcagtacta cctgaccttt     6300

aatttcgaca acgagccgga aaacatttct atcgaaaacc tgagctctga tatcatcggc     6360

cagctggaac tgatgccgaa catcgaacgt ttcccaaacg gtaaaaagta cgagctggac     6420

aaatatacca tgttccacta cctgcgcgcg caggaatttg aacacggcaa atcccgtatc     6480

gcactgacta actccgttaa cgaagctctg ctcaacccgt cccgtgtata caccttcttc     6540

tctagcgact acgtgaaaaa ggtcaacaaa gcgactgaag ctgcaatgtt cttgggttgg     6600

gttgaacagc ttgtttatga ttttaccgac gagacgtccg aagtatctac taccgacaaa     6660

attgcggata tcactatcat catcccgtac atcggtccgg ctctgaacat tggcaacatg     6720

ctgtacaaag acgacttcgt tggcgcactg atcttctccg gtgcggtgat cctgctggag     6780

ttcatcccgg aaatcgccat cccggtactg ggcacctttg ctctggtttc ttacattgca     6840

aacaaggttc tgactgtaca aaccatcgac aacgcgctga gcaaacgtaa cgaaaaatgg     6900

gatgaagttt acaaatatat cgtgaccaac tggctggcta aggttaatac tcagatcgac     6960

ctcatccgca aaaaaatgaa agaagcactg gaaaaccagg cggaagctac caaggcaatc     7020

attaactacc agtacaacca gtacaccgag gaagaaaaaa acaacatcaa cttcaacatc     7080

gacgatctgt cctctaaact gaacgaatcc atcaacaaag ctatgatcaa catcaacaag     7140

ttcctgaacc agtgctctgt aagctatctg atgaactcca tgatcccgta cggtgttaaa     7200

cgtctggagg acttcgatgc gtctctgaaa gacgccctgc tgaaatacat ttacgacaac     7260

cgtggcactc tgatcggtca ggttgatcgt ctgaaggaca aagtgaacaa taccttatcg     7320

accgacatcc cttttcagct cagtaaatat gtcgataacc aacgcctttt gtccactcta     7380

gaaggcggtg gcggtagcgg tggcggtggc agcggcggtg gcggtagcgc actagacaac     7440

agcgacccta aatgcccact aagtcatgaa ggatactgcc ttaatgatgg tgtttgtatg     7500

tacataggaa cattggaccg ttatgcttgc aattgtgtag tgggctatgt cggggaaagg     7560

tgtcaatatc gagatctcaa gctggcagag ttaagagggc tagaagcaca ccatcatcac     7620

caccatcacc atcaccatta atgaaagctt gcggccgcac tcgagcacca ccaccaccac     7680

cactgagatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct     7740

gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg     7800

aaaggaggaa ctatatccgg at                                              7822


<210>  10
<211>  1202
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Polypeptide sequence of EGF-liganded polypeptide GFP-tagged

<400>  10

Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu 
1               5                   10                  15      


Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly 
            20                  25                  30          


Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile 
        35                  40                  45              


Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr 
    50                  55                  60                  


Leu Thr Tyr Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys 
65                  70                  75                  80  


Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu 
                85                  90                  95      


Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu 
            100                 105                 110         


Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly 
        115                 120                 125             


Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr 
    130                 135                 140                 


Asn Tyr Asn Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn 
145                 150                 155                 160 


Gly Ile Lys Val Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser 
                165                 170                 175     


Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly 
            180                 185                 190         


Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Ala Leu 
        195                 200                 205             


Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe 
    210                 215                 220                 


Val Thr Ala Ala Gly Ile Thr His Gly Met Asp Glu Leu Tyr Lys Gly 
225                 230                 235                 240 


Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser Met Glu Phe Val Asn Lys 
                245                 250                 255     


Gln Phe Asn Tyr Lys Asp Pro Val Asn Gly Val Asp Ile Ala Tyr Ile 
            260                 265                 270         


Lys Ile Pro Asn Ala Gly Gln Met Gln Pro Val Lys Ala Phe Lys Ile 
        275                 280                 285             


His Asn Lys Ile Trp Val Ile Pro Glu Arg Asp Thr Phe Thr Asn Pro 
    290                 295                 300                 


Glu Glu Gly Asp Leu Asn Pro Pro Pro Glu Ala Lys Gln Val Pro Val 
305                 310                 315                 320 


Ser Tyr Tyr Asp Ser Thr Tyr Leu Ser Thr Asp Asn Glu Lys Asp Asn 
                325                 330                 335     


Tyr Leu Lys Gly Val Thr Lys Leu Phe Glu Arg Ile Tyr Ser Thr Asp 
            340                 345                 350         


Leu Gly Arg Met Leu Leu Thr Ser Ile Val Arg Gly Ile Pro Phe Trp 
        355                 360                 365             


Gly Gly Ser Thr Ile Asp Thr Glu Leu Lys Val Ile Asp Thr Asn Cys 
    370                 375                 380                 


Ile Asn Val Ile Gln Pro Asp Gly Ser Tyr Arg Ser Glu Glu Leu Asn 
385                 390                 395                 400 


Leu Val Ile Ile Gly Pro Ser Ala Asp Ile Ile Gln Phe Glu Cys Lys 
                405                 410                 415     


Ser Phe Gly His Glu Val Leu Asn Leu Thr Arg Asn Gly Tyr Gly Ser 
            420                 425                 430         


Thr Gln Tyr Ile Arg Phe Ser Pro Asp Phe Thr Phe Gly Phe Glu Glu 
        435                 440                 445             


Ser Leu Glu Val Asp Thr Asn Pro Leu Leu Gly Ala Gly Lys Phe Ala 
    450                 455                 460                 


Thr Asp Pro Ala Val Thr Leu Ala His Glu Leu Ile His Ala Gly His 
465                 470                 475                 480 


Arg Leu Tyr Gly Ile Ala Ile Asn Pro Asn Arg Val Phe Lys Val Asn 
                485                 490                 495     


Thr Asn Ala Tyr Tyr Glu Met Ser Gly Leu Glu Val Ser Phe Glu Glu 
            500                 505                 510         


Leu Arg Thr Phe Gly Gly His Asp Ala Lys Phe Ile Asp Ser Leu Gln 
        515                 520                 525             


Glu Asn Glu Phe Arg Leu Tyr Tyr Tyr Asn Lys Phe Lys Asp Ile Ala 
    530                 535                 540                 


Ser Thr Leu Asn Lys Ala Lys Ser Ile Val Gly Thr Thr Ala Ser Leu 
545                 550                 555                 560 


Gln Tyr Met Lys Asn Val Phe Lys Glu Lys Tyr Leu Leu Ser Glu Asp 
                565                 570                 575     


Thr Ser Gly Lys Phe Ser Val Asp Lys Leu Lys Phe Asp Lys Leu Tyr 
            580                 585                 590         


Lys Met Leu Thr Glu Ile Tyr Thr Glu Asp Asn Phe Val Lys Phe Phe 
        595                 600                 605             


Lys Val Leu Asn Arg Lys Thr Tyr Leu Asn Phe Asp Lys Ala Val Phe 
    610                 615                 620                 


Lys Ile Asn Ile Val Pro Lys Val Asn Tyr Thr Ile Tyr Asp Gly Phe 
625                 630                 635                 640 


Asn Leu Arg Asn Thr Asn Leu Ala Ala Asn Phe Asn Gly Gln Asn Thr 
                645                 650                 655     


Glu Ile Asn Asn Met Asn Phe Thr Lys Leu Lys Asn Phe Thr Gly Leu 
            660                 665                 670         


Phe Glu Phe Tyr Lys Leu Leu Cys Val Asp Gly Ile Ile Thr Ser Lys 
        675                 680                 685             


Thr Lys Ser Leu Ile Glu Gly Arg Asn Lys Ala Leu Asn Leu Gln Cys 
    690                 695                 700                 


Ile Lys Val Asn Asn Trp Asp Leu Phe Phe Ser Pro Ser Glu Asp Asn 
705                 710                 715                 720 


Phe Thr Asn Asp Leu Asn Lys Gly Glu Glu Ile Thr Ser Asp Thr Asn 
                725                 730                 735     


Ile Glu Ala Ala Glu Glu Asn Ile Ser Leu Asp Leu Ile Gln Gln Tyr 
            740                 745                 750         


Tyr Leu Thr Phe Asn Phe Asp Asn Glu Pro Glu Asn Ile Ser Ile Glu 
        755                 760                 765             


Asn Leu Ser Ser Asp Ile Ile Gly Gln Leu Glu Leu Met Pro Asn Ile 
    770                 775                 780                 


Glu Arg Phe Pro Asn Gly Lys Lys Tyr Glu Leu Asp Lys Tyr Thr Met 
785                 790                 795                 800 


Phe His Tyr Leu Arg Ala Gln Glu Phe Glu His Gly Lys Ser Arg Ile 
                805                 810                 815     


Ala Leu Thr Asn Ser Val Asn Glu Ala Leu Leu Asn Pro Ser Arg Val 
            820                 825                 830         


Tyr Thr Phe Phe Ser Ser Asp Tyr Val Lys Lys Val Asn Lys Ala Thr 
        835                 840                 845             


Glu Ala Ala Met Phe Leu Gly Trp Val Glu Gln Leu Val Tyr Asp Phe 
    850                 855                 860                 


Thr Asp Glu Thr Ser Glu Val Ser Thr Thr Asp Lys Ile Ala Asp Ile 
865                 870                 875                 880 


Thr Ile Ile Ile Pro Tyr Ile Gly Pro Ala Leu Asn Ile Gly Asn Met 
                885                 890                 895     


Leu Tyr Lys Asp Asp Phe Val Gly Ala Leu Ile Phe Ser Gly Ala Val 
            900                 905                 910         


Ile Leu Leu Glu Phe Ile Pro Glu Ile Ala Ile Pro Val Leu Gly Thr 
        915                 920                 925             


Phe Ala Leu Val Ser Tyr Ile Ala Asn Lys Val Leu Thr Val Gln Thr 
    930                 935                 940                 


Ile Asp Asn Ala Leu Ser Lys Arg Asn Glu Lys Trp Asp Glu Val Tyr 
945                 950                 955                 960 


Lys Tyr Ile Val Thr Asn Trp Leu Ala Lys Val Asn Thr Gln Ile Asp 
                965                 970                 975     


Leu Ile Arg Lys Lys Met Lys Glu Ala Leu Glu Asn Gln Ala Glu Ala 
            980                 985                 990         


Thr Lys Ala Ile Ile Asn Tyr Gln  Tyr Asn Gln Tyr Thr  Glu Glu Glu 
        995                 1000                 1005             


Lys Asn  Asn Ile Asn Phe Asn  Ile Asp Asp Leu Ser  Ser Lys Leu 
    1010                 1015                 1020             


Asn Glu  Ser Ile Asn Lys Ala  Met Ile Asn Ile Asn  Lys Phe Leu 
    1025                 1030                 1035             


Asn Gln  Cys Ser Val Ser Tyr  Leu Met Asn Ser Met  Ile Pro Tyr 
    1040                 1045                 1050             


Gly Val  Lys Arg Leu Glu Asp  Phe Asp Ala Ser Leu  Lys Asp Ala 
    1055                 1060                 1065             


Leu Leu  Lys Tyr Ile Tyr Asp  Asn Arg Gly Thr Leu  Ile Gly Gln 
    1070                 1075                 1080             


Val Asp  Arg Leu Lys Asp Lys  Val Asn Asn Thr Leu  Ser Thr Asp 
    1085                 1090                 1095             


Ile Pro  Phe Gln Leu Ser Lys  Tyr Val Asp Asn Gln  Arg Leu Leu 
    1100                 1105                 1110             


Ser Thr  Leu Glu Gly Gly Gly  Gly Ser Gly Gly Gly  Gly Ser Gly 
    1115                 1120                 1125             


Gly Gly  Gly Ser Ala Leu Asp  Asn Ser Asp Pro Lys  Cys Pro Leu 
    1130                 1135                 1140             


Ser His  Glu Gly Tyr Cys Leu  Asn Asp Gly Val Cys  Met Tyr Ile 
    1145                 1150                 1155             


Gly Thr  Leu Asp Arg Tyr Ala  Cys Asn Cys Val Val  Gly Tyr Val 
    1160                 1165                 1170             


Gly Glu  Arg Cys Gln Tyr Arg  Asp Leu Lys Leu Ala  Glu Leu Arg 
    1175                 1180                 1185             


Gly Leu  Glu Ala His His His  His His His His His  His His 
    1190                 1195                 1200         


<210>  11
<211>  7651
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of EGF-liganded polypeptide SNAP tagged

<400>  11
tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg       60

cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc      120

ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg      180

gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc      240

acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt      300

ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc      360

ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta      420

acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt      480

cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat      540

ccgctcatga attaattctt agaaaaactc atcgagcatc aaatgaaact gcaatttatt      600

catatcagga ttatcaatac catatttttg aaaaagccgt ttctgtaatg aaggagaaaa      660

ctcaccgagg cagttccata ggatggcaag atcctggtat cggtctgcga ttccgactcg      720

tccaacatca atacaaccta ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa      780

atcaccatga gtgacgactg aatccggtga gaatggcaaa agtttatgca tttctttcca      840

gacttgttca acaggccagc cattacgctc gtcatcaaaa tcactcgcat caaccaaacc      900

gttattcatt cgtgattgcg cctgagcgag acgaaatacg cgatcgctgt taaaaggaca      960

attacaaaca ggaatcgaat gcaaccggcg caggaacact gccagcgcat caacaatatt     1020

ttcacctgaa tcaggatatt cttctaatac ctggaatgct gttttcccgg ggatcgcagt     1080

ggtgagtaac catgcatcat caggagtacg gataaaatgc ttgatggtcg gaagaggcat     1140

aaattccgtc agccagttta gtctgaccat ctcatctgta acatcattgg caacgctacc     1200

tttgccatgt ttcagaaaca actctggcgc atcgggcttc ccatacaatc gatagattgt     1260

cgcacctgat tgcccgacat tatcgcgagc ccatttatac ccatataaat cagcatccat     1320

gttggaattt aatcgcggcc tagagcaaga cgtttcccgt tgaatatggc tcataacacc     1380

ccttgtatta ctgtttatgt aagcagacag ttttattgtt catgaccaaa atcccttaac     1440

gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag     1500

atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg     1560

tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca     1620

gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga     1680

actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca     1740

gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc     1800

agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca     1860

ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa     1920

aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc     1980

cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc     2040

gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg     2100

cctttttacg gttcctggcc ttttgctggc cttttgctca catcggcgat aatggcctgc     2160

ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag     2220

attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg     2280

ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca     2340

gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg     2400

aaggctctca agggcatcgg tcgagatccc ggtgcctaat gagtgagcta acttacatta     2460

attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa     2520

tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgccaggg tggtttttct     2580

tttcaccagt gagacgggca acagctgatt gcccttcacc gcctggccct gagagagttg     2640

cagcaagcgg tccacgctgg tttgccccag caggcgaaaa tcctgtttga tggtggttaa     2700

cggcgggata taacatgagc tgtcttcggt atcgtcgtat cccactaccg agatatccgc     2760

accaacgcgc agcccggact cggtaatggc gcgcattgcg cccagcgcca tctgatcgtt     2820

ggcaaccagc atcgcagtgg gaacgatgcc ctcattcagc atttgcatgg tttgttgaaa     2880

accggacatg gcactccagt cgccttcccg ttccgctatc ggctgaattt gattgcgagt     2940

gagatattta tgccagccag ccagacgcag acgcgccgag acagaactta atgggcccgc     3000

taacagcgcg atttgctggt gacccaatgc gaccagatgc tccacgccca gtcgcgtacc     3060

gtcttcatgg gagaaaataa tactgttgat gggtgtctgg tcagagacat caagaaataa     3120

cgccggaaca ttagtgcagg cagcttccac agcaatggca tcctggtcat ccagcggata     3180

gttaatgatc agcccactga cgcgttgcgc gagaagattg tgcaccgccg ctttacaggc     3240

ttcgacgccg cttcgttcta ccatcgacac caccacgctg gcacccagtt gatcggcgcg     3300

agatttaatc gccgcgacaa tttgcgacgg cgcgtgcagg gccagactgg aggtggcaac     3360

gccaatcagc aacgactgtt tgcccgccag ttgttgtgcc acgcggttgg gaatgtaatt     3420

cagctccgcc atcgccgctt ccactttttc ccgcgttttc gcagaaacgt ggctggcctg     3480

gttcaccacg cgggaaacgg tctgataaga gacaccggca tactctgcga catcgtataa     3540

cgttactggt ttcacattca ccaccctgaa ttgactctct tccgggcgct atcatgccat     3600

accgcgaaag gttttgcgcc attcgatggt gtccgggatc tcgacgctct cccttatgcg     3660

actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa     3720

ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca     3780

tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg     3840

tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga     3900

tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa ttaatacgac tcactatagg     3960

ggaattgtga gcggataaca attcccctca agaaataatt ttgtttaact ttaagaagga     4020

gatatacata tgatggacaa agactgcgaa atgaagcgca ccaccctgga tagccctctg     4080

ggcaagctgg aactgtctgg gtgcgaacag ggcctgcacc gtatcatctt cctgggcaaa     4140

ggaacatctg ccgccgacgc cgtggaagtg cctgccccag ccgccgtgct gggcggacca     4200

gagccactga tgcaggccac cgcctggctc aacgcctact ttcaccagcc tgaggccatc     4260

gaggagttcc ctgtgccagc cctgcaccac ccagtgttcc agcaggagag ctttacccgc     4320

caggtgctgt ggaaactgct gaaagtggtg aagttcggag aggtcatcag ctacagccac     4380

ctggccgccc tggccggcaa tcccgccgcc accgccgccg tgaaaaccgc cctgagcgga     4440

aatcccgtgc ccattctgat cccctgccac cgggtggtgc agggcgacct ggacgtgggg     4500

ggctacgagg gcgggctcgc cgtgaaagag tggctgctgg cccacgaggg ccacagactg     4560

ggcaagcctg ggctgggtgg cggcagcggc ggcggcagcg gcggcggatc catggagttc     4620

gttaacaaac agttcaacta taaagaccca gttaacggtg ttgacattgc ttacatcaaa     4680

atcccgaacg ctggccagat gcagccggta aaggcattca aaatccacaa caaaatctgg     4740

gttatcccgg aacgtgatac ctttactaac ccggaagaag gtgacctgaa cccgccaccg     4800

gaagcgaaac aggtgccggt atcttactat gactccacct acctgtctac cgataacgaa     4860

aaggacaact acctgaaagg tgttactaaa ctgttcgagc gtatttactc caccgacctg     4920

ggccgtatgc tgctgactag catcgttcgc ggtatcccgt tctggggcgg ttctaccatc     4980

gataccgaac tgaaagtaat cgacactaac tgcatcaacg ttattcagcc ggacggttcc     5040

tatcgttccg aagaactgaa cctggtgatc atcggcccgt ctgctgatat catccagttc     5100

gagtgtaaga gctttggtca cgaagttctg aacctcaccc gtaacggcta cggttccact     5160

cagtacatcc gtttctctcc ggacttcacc ttcggttttg aagaatccct ggaagtagac     5220

acgaacccac tgctgggcgc tggtaaattc gcaactgatc ctgcggttac cctggctcac     5280

gaactgattc atgcaggcca ccgcctgtac ggtatcgcca tcaatccgaa ccgtgtcttc     5340

aaagttaaca ccaacgcgta ttacgagatg tccggtctgg aagttagctt cgaagaactg     5400

cgtacttttg gcggtcacga cgctaaattc atcgactctc tgcaagaaaa cgagttccgt     5460

ctgtactact ataacaagtt caaagatatc gcatccaccc tgaacaaagc gaaatccatc     5520

gtgggtacca ctgcttctct ccagtacatg aagaacgttt ttaaagaaaa atacctgctc     5580

agcgaagaca cctccggcaa attctctgta gacaagttga aattcgataa actttacaaa     5640

atgctgactg aaatttacac cgaagacaac ttcgttaagt tctttaaagt tctgaaccgc     5700

aaaacctatc tgaacttcga caaggcagta ttcaaaatca acatcgtgcc gaaagttaac     5760

tacactatct acgatggttt caacctgcgt aacaccaacc tggctgctaa ttttaacggc     5820

cagaacacgg aaatcaacaa catgaacttc acaaaactga aaaacttcac tggtctgttc     5880

gagttttaca agctgctgtg cgtcgacggc atcattacct ccaaaactaa atctctgata     5940

gaaggtagaa acaaagcgct gaacctgcag tgtatcaagg ttaacaactg ggatttattc     6000

ttcagcccga gtgaagacaa cttcaccaac gacctgaaca aaggtgaaga aatcacctca     6060

gatactaaca tcgaagcagc cgaagaaaac atctcgctgg acctgatcca gcagtactac     6120

ctgaccttta atttcgacaa cgagccggaa aacatttcta tcgaaaacct gagctctgat     6180

atcatcggcc agctggaact gatgccgaac atcgaacgtt tcccaaacgg taaaaagtac     6240

gagctggaca aatataccat gttccactac ctgcgcgcgc aggaatttga acacggcaaa     6300

tcccgtatcg cactgactaa ctccgttaac gaagctctgc tcaacccgtc ccgtgtatac     6360

accttcttct ctagcgacta cgtgaaaaag gtcaacaaag cgactgaagc tgcaatgttc     6420

ttgggttggg ttgaacagct tgtttatgat tttaccgacg agacgtccga agtatctact     6480

accgacaaaa ttgcggatat cactatcatc atcccgtaca tcggtccggc tctgaacatt     6540

ggcaacatgc tgtacaaaga cgacttcgtt ggcgcactga tcttctccgg tgcggtgatc     6600

ctgctggagt tcatcccgga aatcgccatc ccggtactgg gcacctttgc tctggtttct     6660

tacattgcaa acaaggttct gactgtacaa accatcgaca acgcgctgag caaacgtaac     6720

gaaaaatggg atgaagttta caaatatatc gtgaccaact ggctggctaa ggttaatact     6780

cagatcgacc tcatccgcaa aaaaatgaaa gaagcactgg aaaaccaggc ggaagctacc     6840

aaggcaatca ttaactacca gtacaaccag tacaccgagg aagaaaaaaa caacatcaac     6900

ttcaacatcg acgatctgtc ctctaaactg aacgaatcca tcaacaaagc tatgatcaac     6960

atcaacaagt tcctgaacca gtgctctgta agctatctga tgaactccat gatcccgtac     7020

ggtgttaaac gtctggagga cttcgatgcg tctctgaaag acgccctgct gaaatacatt     7080

tacgacaacc gtggcactct gatcggtcag gttgatcgtc tgaaggacaa agtgaacaat     7140

accttatcga ccgacatccc ttttcagctc agtaaatatg tcgataacca acgccttttg     7200

tccactctag aaggcggtgg cggtagcggt ggcggtggca gcggcggtgg cggtagcgca     7260

ctagacaaca gcgaccctaa atgcccacta agtcatgaag gatactgcct taatgatggt     7320

gtttgtatgt acataggaac attggaccgt tatgcttgca attgtgtagt gggctatgtc     7380

ggggaaaggt gtcaatatcg agatctcaag ctggcagagt taagagggct agaagcacac     7440

catcatcacc accatcacca tcaccattaa tgaaagcttg cggccgcact cgagcaccac     7500

caccaccacc actgagatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct     7560

gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt     7620

tttttgctga aaggaggaac tatatccgga t                                    7651


<210>  12
<211>  1145
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Polypeptide sequence of EGF-liganded polypeptide SNAP tagged

<400>  12

Met Asp Lys Asp Cys Glu Met Lys Arg Thr Thr Leu Asp Ser Pro Leu 
1               5                   10                  15      


Gly Lys Leu Glu Leu Ser Gly Cys Glu Gln Gly Leu His Arg Ile Ile 
            20                  25                  30          


Phe Leu Gly Lys Gly Thr Ser Ala Ala Asp Ala Val Glu Val Pro Ala 
        35                  40                  45              


Pro Ala Ala Val Leu Gly Gly Pro Glu Pro Leu Met Gln Ala Thr Ala 
    50                  55                  60                  


Trp Leu Asn Ala Tyr Phe His Gln Pro Glu Ala Ile Glu Glu Phe Pro 
65                  70                  75                  80  


Val Pro Ala Leu His His Pro Val Phe Gln Gln Glu Ser Phe Thr Arg 
                85                  90                  95      


Gln Val Leu Trp Lys Leu Leu Lys Val Val Lys Phe Gly Glu Val Ile 
            100                 105                 110         


Ser Tyr Ser His Leu Ala Ala Leu Ala Gly Asn Pro Ala Ala Thr Ala 
        115                 120                 125             


Ala Val Lys Thr Ala Leu Ser Gly Asn Pro Val Pro Ile Leu Ile Pro 
    130                 135                 140                 


Cys His Arg Val Val Gln Gly Asp Leu Asp Val Gly Gly Tyr Glu Gly 
145                 150                 155                 160 


Gly Leu Ala Val Lys Glu Trp Leu Leu Ala His Glu Gly His Arg Leu 
                165                 170                 175     


Gly Lys Pro Gly Leu Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly 
            180                 185                 190         


Ser Met Glu Phe Val Asn Lys Gln Phe Asn Tyr Lys Asp Pro Val Asn 
        195                 200                 205             


Gly Val Asp Ile Ala Tyr Ile Lys Ile Pro Asn Ala Gly Gln Met Gln 
    210                 215                 220                 


Pro Val Lys Ala Phe Lys Ile His Asn Lys Ile Trp Val Ile Pro Glu 
225                 230                 235                 240 


Arg Asp Thr Phe Thr Asn Pro Glu Glu Gly Asp Leu Asn Pro Pro Pro 
                245                 250                 255     


Glu Ala Lys Gln Val Pro Val Ser Tyr Tyr Asp Ser Thr Tyr Leu Ser 
            260                 265                 270         


Thr Asp Asn Glu Lys Asp Asn Tyr Leu Lys Gly Val Thr Lys Leu Phe 
        275                 280                 285             


Glu Arg Ile Tyr Ser Thr Asp Leu Gly Arg Met Leu Leu Thr Ser Ile 
    290                 295                 300                 


Val Arg Gly Ile Pro Phe Trp Gly Gly Ser Thr Ile Asp Thr Glu Leu 
305                 310                 315                 320 


Lys Val Ile Asp Thr Asn Cys Ile Asn Val Ile Gln Pro Asp Gly Ser 
                325                 330                 335     


Tyr Arg Ser Glu Glu Leu Asn Leu Val Ile Ile Gly Pro Ser Ala Asp 
            340                 345                 350         


Ile Ile Gln Phe Glu Cys Lys Ser Phe Gly His Glu Val Leu Asn Leu 
        355                 360                 365             


Thr Arg Asn Gly Tyr Gly Ser Thr Gln Tyr Ile Arg Phe Ser Pro Asp 
    370                 375                 380                 


Phe Thr Phe Gly Phe Glu Glu Ser Leu Glu Val Asp Thr Asn Pro Leu 
385                 390                 395                 400 


Leu Gly Ala Gly Lys Phe Ala Thr Asp Pro Ala Val Thr Leu Ala His 
                405                 410                 415     


Glu Leu Ile His Ala Gly His Arg Leu Tyr Gly Ile Ala Ile Asn Pro 
            420                 425                 430         


Asn Arg Val Phe Lys Val Asn Thr Asn Ala Tyr Tyr Glu Met Ser Gly 
        435                 440                 445             


Leu Glu Val Ser Phe Glu Glu Leu Arg Thr Phe Gly Gly His Asp Ala 
    450                 455                 460                 


Lys Phe Ile Asp Ser Leu Gln Glu Asn Glu Phe Arg Leu Tyr Tyr Tyr 
465                 470                 475                 480 


Asn Lys Phe Lys Asp Ile Ala Ser Thr Leu Asn Lys Ala Lys Ser Ile 
                485                 490                 495     


Val Gly Thr Thr Ala Ser Leu Gln Tyr Met Lys Asn Val Phe Lys Glu 
            500                 505                 510         


Lys Tyr Leu Leu Ser Glu Asp Thr Ser Gly Lys Phe Ser Val Asp Lys 
        515                 520                 525             


Leu Lys Phe Asp Lys Leu Tyr Lys Met Leu Thr Glu Ile Tyr Thr Glu 
    530                 535                 540                 


Asp Asn Phe Val Lys Phe Phe Lys Val Leu Asn Arg Lys Thr Tyr Leu 
545                 550                 555                 560 


Asn Phe Asp Lys Ala Val Phe Lys Ile Asn Ile Val Pro Lys Val Asn 
                565                 570                 575     


Tyr Thr Ile Tyr Asp Gly Phe Asn Leu Arg Asn Thr Asn Leu Ala Ala 
            580                 585                 590         


Asn Phe Asn Gly Gln Asn Thr Glu Ile Asn Asn Met Asn Phe Thr Lys 
        595                 600                 605             


Leu Lys Asn Phe Thr Gly Leu Phe Glu Phe Tyr Lys Leu Leu Cys Val 
    610                 615                 620                 


Asp Gly Ile Ile Thr Ser Lys Thr Lys Ser Leu Ile Glu Gly Arg Asn 
625                 630                 635                 640 


Lys Ala Leu Asn Leu Gln Cys Ile Lys Val Asn Asn Trp Asp Leu Phe 
                645                 650                 655     


Phe Ser Pro Ser Glu Asp Asn Phe Thr Asn Asp Leu Asn Lys Gly Glu 
            660                 665                 670         


Glu Ile Thr Ser Asp Thr Asn Ile Glu Ala Ala Glu Glu Asn Ile Ser 
        675                 680                 685             


Leu Asp Leu Ile Gln Gln Tyr Tyr Leu Thr Phe Asn Phe Asp Asn Glu 
    690                 695                 700                 


Pro Glu Asn Ile Ser Ile Glu Asn Leu Ser Ser Asp Ile Ile Gly Gln 
705                 710                 715                 720 


Leu Glu Leu Met Pro Asn Ile Glu Arg Phe Pro Asn Gly Lys Lys Tyr 
                725                 730                 735     


Glu Leu Asp Lys Tyr Thr Met Phe His Tyr Leu Arg Ala Gln Glu Phe 
            740                 745                 750         


Glu His Gly Lys Ser Arg Ile Ala Leu Thr Asn Ser Val Asn Glu Ala 
        755                 760                 765             


Leu Leu Asn Pro Ser Arg Val Tyr Thr Phe Phe Ser Ser Asp Tyr Val 
    770                 775                 780                 


Lys Lys Val Asn Lys Ala Thr Glu Ala Ala Met Phe Leu Gly Trp Val 
785                 790                 795                 800 


Glu Gln Leu Val Tyr Asp Phe Thr Asp Glu Thr Ser Glu Val Ser Thr 
                805                 810                 815     


Thr Asp Lys Ile Ala Asp Ile Thr Ile Ile Ile Pro Tyr Ile Gly Pro 
            820                 825                 830         


Ala Leu Asn Ile Gly Asn Met Leu Tyr Lys Asp Asp Phe Val Gly Ala 
        835                 840                 845             


Leu Ile Phe Ser Gly Ala Val Ile Leu Leu Glu Phe Ile Pro Glu Ile 
    850                 855                 860                 


Ala Ile Pro Val Leu Gly Thr Phe Ala Leu Val Ser Tyr Ile Ala Asn 
865                 870                 875                 880 


Lys Val Leu Thr Val Gln Thr Ile Asp Asn Ala Leu Ser Lys Arg Asn 
                885                 890                 895     


Glu Lys Trp Asp Glu Val Tyr Lys Tyr Ile Val Thr Asn Trp Leu Ala 
            900                 905                 910         


Lys Val Asn Thr Gln Ile Asp Leu Ile Arg Lys Lys Met Lys Glu Ala 
        915                 920                 925             


Leu Glu Asn Gln Ala Glu Ala Thr Lys Ala Ile Ile Asn Tyr Gln Tyr 
    930                 935                 940                 


Asn Gln Tyr Thr Glu Glu Glu Lys Asn Asn Ile Asn Phe Asn Ile Asp 
945                 950                 955                 960 


Asp Leu Ser Ser Lys Leu Asn Glu Ser Ile Asn Lys Ala Met Ile Asn 
                965                 970                 975     


Ile Asn Lys Phe Leu Asn Gln Cys Ser Val Ser Tyr Leu Met Asn Ser 
            980                 985                 990         


Met Ile Pro Tyr Gly Val Lys Arg  Leu Glu Asp Phe Asp  Ala Ser Leu 
        995                 1000                 1005             


Lys Asp  Ala Leu Leu Lys Tyr  Ile Tyr Asp Asn Arg  Gly Thr Leu 
    1010                 1015                 1020             


Ile Gly  Gln Val Asp Arg Leu  Lys Asp Lys Val Asn  Asn Thr Leu 
    1025                 1030                 1035             


Ser Thr  Asp Ile Pro Phe Gln  Leu Ser Lys Tyr Val  Asp Asn Gln 
    1040                 1045                 1050             


Arg Leu  Leu Ser Thr Leu Glu  Gly Gly Gly Gly Ser  Gly Gly Gly 
    1055                 1060                 1065             


Gly Ser  Gly Gly Gly Gly Ser  Ala Leu Asp Asn Ser  Asp Pro Lys 
    1070                 1075                 1080             


Cys Pro  Leu Ser His Glu Gly  Tyr Cys Leu Asn Asp  Gly Val Cys 
    1085                 1090                 1095             


Met Tyr  Ile Gly Thr Leu Asp  Arg Tyr Ala Cys Asn  Cys Val Val 
    1100                 1105                 1110             


Gly Tyr  Val Gly Glu Arg Cys  Gln Tyr Arg Asp Leu  Lys Leu Ala 
    1115                 1120                 1125             


Glu Leu  Arg Gly Leu Glu Ala  His His His His His  His His His 
    1130                 1135                 1140             


His His  
    1145 


<210>  13
<211>  4683
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of Sortase A (LPESG-targeting)

<400>  13
tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg       60

cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc      120

ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg      180

gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc      240

acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt      300

ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc      360

ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta      420

acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt      480

cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat      540

ccgctcatga attaattctt agaaaaactc atcgagcatc aaatgaaact gcaatttatt      600

catatcagga ttatcaatac catatttttg aaaaagccgt ttctgtaatg aaggagaaaa      660

ctcaccgagg cagttccata ggatggcaag atcctggtat cggtctgcga ttccgactcg      720

tccaacatca atacaaccta ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa      780

atcaccatga gtgacgactg aatccggtga gaatggcaaa agtttatgca tttctttcca      840

gacttgttca acaggccagc cattacgctc gtcatcaaaa tcactcgcat caaccaaacc      900

gttattcatt cgtgattgcg cctgagcgag acgaaatacg cgatcgctgt taaaaggaca      960

attacaaaca ggaatcgaat gcaaccggcg caggaacact gccagcgcat caacaatatt     1020

ttcacctgaa tcaggatatt cttctaatac ctggaatgct gttttcccgg ggatcgcagt     1080

ggtgagtaac catgcatcat caggagtacg gataaaatgc ttgatggtcg gaagaggcat     1140

aaattccgtc agccagttta gtctgaccat ctcatctgta acatcattgg caacgctacc     1200

tttgccatgt ttcagaaaca actctggcgc atcgggcttc ccatacaatc gatagattgt     1260

cgcacctgat tgcccgacat tatcgcgagc ccatttatac ccatataaat cagcatccat     1320

gttggaattt aatcgcggcc tagagcaaga cgtttcccgt tgaatatggc tcataacacc     1380

ccttgtatta ctgtttatgt aagcagacag ttttattgtt catgaccaaa atcccttaac     1440

gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag     1500

atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg     1560

tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca     1620

gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga     1680

actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca     1740

gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc     1800

agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca     1860

ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa     1920

aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc     1980

cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc     2040

gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg     2100

cctttttacg gttcctggcc ttttgctggc cttttgctca catcggcgat aatggcctgc     2160

ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag     2220

attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg     2280

ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca     2340

gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg     2400

aaggctctca agggcatcgg tcgagatccc ggtgcctaat gagtgagcta acttacatta     2460

attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa     2520

tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgccaggg tggtttttct     2580

tttcaccagt gagacgggca acagctgatt gcccttcacc gcctggccct gagagagttg     2640

cagcaagcgg tccacgctgg tttgccccag caggcgaaaa tcctgtttga tggtggttaa     2700

cggcgggata taacatgagc tgtcttcggt atcgtcgtat cccactaccg agatatccgc     2760

accaacgcgc agcccggact cggtaatggc gcgcattgcg cccagcgcca tctgatcgtt     2820

ggcaaccagc atcgcagtgg gaacgatgcc ctcattcagc atttgcatgg tttgttgaaa     2880

accggacatg gcactccagt cgccttcccg ttccgctatc ggctgaattt gattgcgagt     2940

gagatattta tgccagccag ccagacgcag acgcgccgag acagaactta atgggcccgc     3000

taacagcgcg atttgctggt gacccaatgc gaccagatgc tccacgccca gtcgcgtacc     3060

gtcttcatgg gagaaaataa tactgttgat gggtgtctgg tcagagacat caagaaataa     3120

cgccggaaca ttagtgcagg cagcttccac agcaatggca tcctggtcat ccagcggata     3180

gttaatgatc agcccactga cgcgttgcgc gagaagattg tgcaccgccg ctttacaggc     3240

ttcgacgccg cttcgttcta ccatcgacac caccacgctg gcacccagtt gatcggcgcg     3300

agatttaatc gccgcgacaa tttgcgacgg cgcgtgcagg gccagactgg aggtggcaac     3360

gccaatcagc aacgactgtt tgcccgccag ttgttgtgcc acgcggttgg gaatgtaatt     3420

cagctccgcc atcgccgctt ccactttttc ccgcgttttc gcagaaacgt ggctggcctg     3480

gttcaccacg cgggaaacgg tctgataaga gacaccggca tactctgcga catcgtataa     3540

cgttactggt ttcacattca ccaccctgaa ttgactctct tccgggcgct atcatgccat     3600

accgcgaaag gttttgcgcc attcgatggt gtccgggatc tcgacgctct cccttatgcg     3660

actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa     3720

ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca     3780

tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg     3840

tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga     3900

tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa ttaatacgac tcactatagg     3960

ggaattgtga gcggataaca attcccctca agaaataatt ttgtttaact ttaagaagga     4020

gatatcatat gcaggcaaaa ccgcagattc cgaaagataa aagcaaagtg gcaggctata     4080

ttgaaattcc ggatgccgat attaaagaac cggtttatcc gggtcctgca acacgtgaac     4140

agctggatcg tggtgtttgt tttgttgaag aaaatgagag cctggatgat cagaacatta     4200

gcattaccgg tcataccgca attgatcgtc cgaattatca gtttaccaat ctgcgtgcag     4260

ccaaaccggg tagcatggtt tatctgaaag ttggtaatga aacccgcatc tacaaaatga     4320

ccagcattcg taatgttaaa ccgaccgcag ttggtgttct ggatgaacaa aaaggtaaag     4380

ataaacagct gaccctggtt acctgtgatg attataactt tgaaaccggt gtttgggaaa     4440

cgcgcaaaat ctttgttgca accgaagtta aacatcacca tcaccaccat catcatcacc     4500

attaaaagct tgcggccgca ctcgagcacc accaccacca ccactgagat ccggctgcta     4560

acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac     4620

cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg     4680

gat                                                                   4683


<210>  14
<211>  158
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Polypeptide sequence of Sortase A (LPESG-targeting)

<400>  14

Met Gln Ala Lys Pro Gln Ile Pro Lys Asp Lys Ser Lys Val Ala Gly 
1               5                   10                  15      


Tyr Ile Glu Ile Pro Asp Ala Asp Ile Lys Glu Pro Val Tyr Pro Gly 
            20                  25                  30          


Pro Ala Thr Arg Glu Gln Leu Asp Arg Gly Val Cys Phe Val Glu Glu 
        35                  40                  45              


Asn Glu Ser Leu Asp Asp Gln Asn Ile Ser Ile Thr Gly His Thr Ala 
    50                  55                  60                  


Ile Asp Arg Pro Asn Tyr Gln Phe Thr Asn Leu Arg Ala Ala Lys Pro 
65                  70                  75                  80  


Gly Ser Met Val Tyr Leu Lys Val Gly Asn Glu Thr Arg Ile Tyr Lys 
                85                  90                  95      


Met Thr Ser Ile Arg Asn Val Lys Pro Thr Ala Val Gly Val Leu Asp 
            100                 105                 110         


Glu Gln Lys Gly Lys Asp Lys Gln Leu Thr Leu Val Thr Cys Asp Asp 
        115                 120                 125             


Tyr Asn Phe Glu Thr Gly Val Trp Glu Thr Arg Lys Ile Phe Val Ala 
    130                 135                 140                 


Thr Glu Val Lys His His His His His His His His His His 
145                 150                 155             


<210>  15
<211>  4684
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of Sortase A (LAETG-targeting)

<400>  15
tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg       60

cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc      120

ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg      180

gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc      240

acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt      300

ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc      360

ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta      420

acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt      480

cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat      540

ccgctcatga attaattctt agaaaaactc atcgagcatc aaatgaaact gcaatttatt      600

catatcagga ttatcaatac catatttttg aaaaagccgt ttctgtaatg aaggagaaaa      660

ctcaccgagg cagttccata ggatggcaag atcctggtat cggtctgcga ttccgactcg      720

tccaacatca atacaaccta ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa      780

atcaccatga gtgacgactg aatccggtga gaatggcaaa agtttatgca tttctttcca      840

gacttgttca acaggccagc cattacgctc gtcatcaaaa tcactcgcat caaccaaacc      900

gttattcatt cgtgattgcg cctgagcgag acgaaatacg cgatcgctgt taaaaggaca      960

attacaaaca ggaatcgaat gcaaccggcg caggaacact gccagcgcat caacaatatt     1020

ttcacctgaa tcaggatatt cttctaatac ctggaatgct gttttcccgg ggatcgcagt     1080

ggtgagtaac catgcatcat caggagtacg gataaaatgc ttgatggtcg gaagaggcat     1140

aaattccgtc agccagttta gtctgaccat ctcatctgta acatcattgg caacgctacc     1200

tttgccatgt ttcagaaaca actctggcgc atcgggcttc ccatacaatc gatagattgt     1260

cgcacctgat tgcccgacat tatcgcgagc ccatttatac ccatataaat cagcatccat     1320

gttggaattt aatcgcggcc tagagcaaga cgtttcccgt tgaatatggc tcataacacc     1380

ccttgtatta ctgtttatgt aagcagacag ttttattgtt catgaccaaa atcccttaac     1440

gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag     1500

atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg     1560

tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca     1620

gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga     1680

actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca     1740

gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc     1800

agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca     1860

ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa     1920

aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc     1980

cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc     2040

gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg     2100

cctttttacg gttcctggcc ttttgctggc cttttgctca catcggcgat aatggcctgc     2160

ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag     2220

attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg     2280

ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca     2340

gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg     2400

aaggctctca agggcatcgg tcgagatccc ggtgcctaat gagtgagcta acttacatta     2460

attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa     2520

tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgccaggg tggtttttct     2580

tttcaccagt gagacgggca acagctgatt gcccttcacc gcctggccct gagagagttg     2640

cagcaagcgg tccacgctgg tttgccccag caggcgaaaa tcctgtttga tggtggttaa     2700

cggcgggata taacatgagc tgtcttcggt atcgtcgtat cccactaccg agatatccgc     2760

accaacgcgc agcccggact cggtaatggc gcgcattgcg cccagcgcca tctgatcgtt     2820

ggcaaccagc atcgcagtgg gaacgatgcc ctcattcagc atttgcatgg tttgttgaaa     2880

accggacatg gcactccagt cgccttcccg ttccgctatc ggctgaattt gattgcgagt     2940

gagatattta tgccagccag ccagacgcag acgcgccgag acagaactta atgggcccgc     3000

taacagcgcg atttgctggt gacccaatgc gaccagatgc tccacgccca gtcgcgtacc     3060

gtcttcatgg gagaaaataa tactgttgat gggtgtctgg tcagagacat caagaaataa     3120

cgccggaaca ttagtgcagg cagcttccac agcaatggca tcctggtcat ccagcggata     3180

gttaatgatc agcccactga cgcgttgcgc gagaagattg tgcaccgccg ctttacaggc     3240

ttcgacgccg cttcgttcta ccatcgacac caccacgctg gcacccagtt gatcggcgcg     3300

agatttaatc gccgcgacaa tttgcgacgg cgcgtgcagg gccagactgg aggtggcaac     3360

gccaatcagc aacgactgtt tgcccgccag ttgttgtgcc acgcggttgg gaatgtaatt     3420

cagctccgcc atcgccgctt ccactttttc ccgcgttttc gcagaaacgt ggctggcctg     3480

gttcaccacg cgggaaacgg tctgataaga gacaccggca tactctgcga catcgtataa     3540

cgttactggt ttcacattca ccaccctgaa ttgactctct tccgggcgct atcatgccat     3600

accgcgaaag gttttgcgcc attcgatggt gtccgggatc tcgacgctct cccttatgcg     3660

actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa     3720

ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca     3780

tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg     3840

tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga     3900

tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa ttaatacgac tcactatagg     3960

ggaattgtga gcggataaca attcccctca agaaataatt ttgtttaact ttaagaagga     4020

gatatacata tgcaggcaaa accgcagatt ccgaaagata aaagcaaagt ggcaggctat     4080

attgaaattc cggatgccga tattaaagaa ccggtttatc cgggtcctgc aacacgtgaa     4140

cagctgaatc gtggtgtttg ttttcacgat gaaaatgaga gcctggatga tcagaatatt     4200

agcattgcag gccatacctt tattgatcgt ccgaattatc agttcaccaa tctgaaagca     4260

gcaaaaccgg gtagcatggt ttatttcaaa gttggtaatg aaacccgcat ctacaaaatg     4320

accagcattc gtaaagttca tccgaatgca gttggtgttc tggatgaaca agaaggcaaa     4380

gataaacagc tgaccctggt tacctgtgat gattataacg aagaaaccgg tgtttgggaa     4440

agccgtaaaa tctttgttgc aaccgaagtg aaacatcatc accaccatca ccatcatcat     4500

cactaaaagc ttgcggccgc actcgagcac caccaccacc accactgaga tccggctgct     4560

aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa     4620

ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc     4680

ggat                                                                  4684


<210>  16
<211>  158
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Polypeptide sequence of Sortase A (LAETG-targeting)

<400>  16

Met Gln Ala Lys Pro Gln Ile Pro Lys Asp Lys Ser Lys Val Ala Gly 
1               5                   10                  15      


Tyr Ile Glu Ile Pro Asp Ala Asp Ile Lys Glu Pro Val Tyr Pro Gly 
            20                  25                  30          


Pro Ala Thr Arg Glu Gln Leu Asn Arg Gly Val Cys Phe His Asp Glu 
        35                  40                  45              


Asn Glu Ser Leu Asp Asp Gln Asn Ile Ser Ile Ala Gly His Thr Phe 
    50                  55                  60                  


Ile Asp Arg Pro Asn Tyr Gln Phe Thr Asn Leu Lys Ala Ala Lys Pro 
65                  70                  75                  80  


Gly Ser Met Val Tyr Phe Lys Val Gly Asn Glu Thr Arg Ile Tyr Lys 
                85                  90                  95      


Met Thr Ser Ile Arg Lys Val His Pro Asn Ala Val Gly Val Leu Asp 
            100                 105                 110         


Glu Gln Glu Gly Lys Asp Lys Gln Leu Thr Leu Val Thr Cys Asp Asp 
        115                 120                 125             


Tyr Asn Glu Glu Thr Gly Val Trp Glu Ser Arg Lys Ile Phe Val Ala 
    130                 135                 140                 


Thr Glu Val Lys His His His His His His His His His His 
145                 150                 155             


<210>  17
<211>  1296
<212>  PRT
<213>  Clostridium botulinum

<400>  17

Met Pro Phe Val Asn Lys Gln Phe Asn Tyr Lys Asp Pro Val Asn Gly 
1               5                   10                  15      


Val Asp Ile Ala Tyr Ile Lys Ile Pro Asn Val Gly Gln Met Gln Pro 
            20                  25                  30          


Val Lys Ala Phe Lys Ile His Asn Lys Ile Trp Val Ile Pro Glu Arg 
        35                  40                  45              


Asp Thr Phe Thr Asn Pro Glu Glu Gly Asp Leu Asn Pro Pro Pro Glu 
    50                  55                  60                  


Ala Lys Gln Val Pro Val Ser Tyr Tyr Asp Ser Thr Tyr Leu Ser Thr 
65                  70                  75                  80  


Asp Asn Glu Lys Asp Asn Tyr Leu Lys Gly Val Thr Lys Leu Phe Glu 
                85                  90                  95      


Arg Ile Tyr Ser Thr Asp Leu Gly Arg Met Leu Leu Thr Ser Ile Val 
            100                 105                 110         


Arg Gly Ile Pro Phe Trp Gly Gly Ser Thr Ile Asp Thr Glu Leu Lys 
        115                 120                 125             


Val Ile Asp Thr Asn Cys Ile Asn Val Ile Gln Pro Asp Gly Ser Tyr 
    130                 135                 140                 


Arg Ser Glu Glu Leu Asn Leu Val Ile Ile Gly Pro Ser Ala Asp Ile 
145                 150                 155                 160 


Ile Gln Phe Glu Cys Lys Ser Phe Gly His Glu Val Leu Asn Leu Thr 
                165                 170                 175     


Arg Asn Gly Tyr Gly Ser Thr Gln Tyr Ile Arg Phe Ser Pro Asp Phe 
            180                 185                 190         


Thr Phe Gly Phe Glu Glu Ser Leu Glu Val Asp Thr Asn Pro Leu Leu 
        195                 200                 205             


Gly Ala Gly Lys Phe Ala Thr Asp Pro Ala Val Thr Leu Ala His Glu 
    210                 215                 220                 


Leu Ile His Ala Gly His Arg Leu Tyr Gly Ile Ala Ile Asn Pro Asn 
225                 230                 235                 240 


Arg Val Phe Lys Val Asn Thr Asn Ala Tyr Tyr Glu Met Ser Gly Leu 
                245                 250                 255     


Glu Val Ser Phe Glu Glu Leu Arg Thr Phe Gly Gly His Asp Ala Lys 
            260                 265                 270         


Phe Ile Asp Ser Leu Gln Glu Asn Glu Phe Arg Leu Tyr Tyr Tyr Asn 
        275                 280                 285             


Lys Phe Lys Asp Ile Ala Ser Thr Leu Asn Lys Ala Lys Ser Ile Val 
    290                 295                 300                 


Gly Thr Thr Ala Ser Leu Gln Tyr Met Lys Asn Val Phe Lys Glu Lys 
305                 310                 315                 320 


Tyr Leu Leu Ser Glu Asp Thr Ser Gly Lys Phe Ser Val Asp Lys Leu 
                325                 330                 335     


Lys Phe Asp Lys Leu Tyr Lys Met Leu Thr Glu Ile Tyr Thr Glu Asp 
            340                 345                 350         


Asn Phe Val Lys Phe Phe Lys Val Leu Asn Arg Lys Thr Tyr Leu Asn 
        355                 360                 365             


Phe Asp Lys Ala Val Phe Lys Ile Asn Ile Val Pro Lys Val Asn Tyr 
    370                 375                 380                 


Thr Ile Tyr Asp Gly Phe Asn Leu Arg Asn Thr Asn Leu Ala Ala Asn 
385                 390                 395                 400 


Phe Asn Gly Gln Asn Thr Glu Ile Asn Asn Met Asn Phe Thr Lys Leu 
                405                 410                 415     


Lys Asn Phe Thr Gly Leu Phe Glu Phe Tyr Lys Leu Leu Cys Val Arg 
            420                 425                 430         


Gly Ile Ile Thr Ser Lys Thr Lys Ser Leu Asp Lys Gly Tyr Asn Lys 
        435                 440                 445             


Ala Leu Asn Asp Leu Cys Ile Lys Val Asn Asn Trp Asp Leu Phe Phe 
    450                 455                 460                 


Ser Pro Ser Glu Asp Asn Phe Thr Asn Asp Leu Asn Lys Gly Glu Glu 
465                 470                 475                 480 


Ile Thr Ser Asp Thr Asn Ile Glu Ala Ala Glu Glu Asn Ile Ser Leu 
                485                 490                 495     


Asp Leu Ile Gln Gln Tyr Tyr Leu Thr Phe Asn Phe Asp Asn Glu Pro 
            500                 505                 510         


Glu Asn Ile Ser Ile Glu Asn Leu Ser Ser Asp Ile Ile Gly Gln Leu 
        515                 520                 525             


Glu Leu Met Pro Asn Ile Glu Arg Phe Pro Asn Gly Lys Lys Tyr Glu 
    530                 535                 540                 


Leu Asp Lys Tyr Thr Met Phe His Tyr Leu Arg Ala Gln Glu Phe Glu 
545                 550                 555                 560 


His Gly Lys Ser Arg Ile Ala Leu Thr Asn Ser Val Asn Glu Ala Leu 
                565                 570                 575     


Leu Asn Pro Ser Arg Val Tyr Thr Phe Phe Ser Ser Asp Tyr Val Lys 
            580                 585                 590         


Lys Val Asn Lys Ala Thr Glu Ala Ala Met Phe Leu Gly Trp Val Glu 
        595                 600                 605             


Gln Leu Val Tyr Asp Phe Thr Asp Glu Thr Ser Glu Val Ser Thr Thr 
    610                 615                 620                 


Asp Lys Ile Ala Asp Ile Thr Ile Ile Ile Pro Tyr Ile Gly Pro Ala 
625                 630                 635                 640 


Leu Asn Ile Gly Asn Met Leu Tyr Lys Asp Asp Phe Val Gly Ala Leu 
                645                 650                 655     


Ile Phe Ser Gly Ala Val Ile Leu Leu Glu Phe Ile Pro Glu Ile Ala 
            660                 665                 670         


Ile Pro Val Leu Gly Thr Phe Ala Leu Val Ser Tyr Ile Ala Asn Lys 
        675                 680                 685             


Val Leu Thr Val Gln Thr Ile Asp Asn Ala Leu Ser Lys Arg Asn Glu 
    690                 695                 700                 


Lys Trp Asp Glu Val Tyr Lys Tyr Ile Val Thr Asn Trp Leu Ala Lys 
705                 710                 715                 720 


Val Asn Thr Gln Ile Asp Leu Ile Arg Lys Lys Met Lys Glu Ala Leu 
                725                 730                 735     


Glu Asn Gln Ala Glu Ala Thr Lys Ala Ile Ile Asn Tyr Gln Tyr Asn 
            740                 745                 750         


Gln Tyr Thr Glu Glu Glu Lys Asn Asn Ile Asn Phe Asn Ile Asp Asp 
        755                 760                 765             


Leu Ser Ser Lys Leu Asn Glu Ser Ile Asn Lys Ala Met Ile Asn Ile 
    770                 775                 780                 


Asn Lys Phe Leu Asn Gln Cys Ser Val Ser Tyr Leu Met Asn Ser Met 
785                 790                 795                 800 


Ile Pro Tyr Gly Val Lys Arg Leu Glu Asp Phe Asp Ala Ser Leu Lys 
                805                 810                 815     


Asp Ala Leu Leu Lys Tyr Ile Tyr Asp Asn Arg Gly Thr Leu Ile Gly 
            820                 825                 830         


Gln Val Asp Arg Leu Lys Asp Lys Val Asn Asn Thr Leu Ser Thr Asp 
        835                 840                 845             


Ile Pro Phe Gln Leu Ser Lys Tyr Val Asp Asn Gln Arg Leu Leu Ser 
    850                 855                 860                 


Thr Phe Thr Glu Tyr Ile Lys Asn Ile Ile Asn Thr Ser Ile Leu Asn 
865                 870                 875                 880 


Leu Arg Tyr Glu Ser Asn His Leu Ile Asp Leu Ser Arg Tyr Ala Ser 
                885                 890                 895     


Lys Ile Asn Ile Gly Ser Lys Val Asn Phe Asp Pro Ile Asp Lys Asn 
            900                 905                 910         


Gln Ile Gln Leu Phe Asn Leu Glu Ser Ser Lys Ile Glu Val Ile Leu 
        915                 920                 925             


Lys Asn Ala Ile Val Tyr Asn Ser Met Tyr Glu Asn Phe Ser Thr Ser 
    930                 935                 940                 


Phe Trp Ile Arg Ile Pro Lys Tyr Phe Asn Ser Ile Ser Leu Asn Asn 
945                 950                 955                 960 


Glu Tyr Thr Ile Ile Asn Cys Met Glu Asn Asn Ser Gly Trp Lys Val 
                965                 970                 975     


Ser Leu Asn Tyr Gly Glu Ile Ile Trp Thr Leu Gln Asp Thr Gln Glu 
            980                 985                 990         


Ile Lys Gln Arg Val Val Phe Lys  Tyr Ser Gln Met Ile  Asn Ile Ser 
        995                 1000                 1005             


Asp Tyr  Ile Asn Arg Trp Ile  Phe Val Thr Ile Thr  Asn Asn Arg 
    1010                 1015                 1020             


Leu Asn  Asn Ser Lys Ile Tyr  Ile Asn Gly Arg Leu  Ile Asp Gln 
    1025                 1030                 1035             


Lys Pro  Ile Ser Asn Leu Gly  Asn Ile His Ala Ser  Asn Asn Ile 
    1040                 1045                 1050             


Met Phe  Lys Leu Asp Gly Cys  Arg Asp Thr His Arg  Tyr Ile Trp 
    1055                 1060                 1065             


Ile Lys  Tyr Phe Asn Leu Phe  Asp Lys Glu Leu Asn  Glu Lys Glu 
    1070                 1075                 1080             


Ile Lys  Asp Leu Tyr Asp Asn  Gln Ser Asn Ser Gly  Ile Leu Lys 
    1085                 1090                 1095             


Asp Phe  Trp Gly Asp Tyr Leu  Gln Tyr Asp Lys Pro  Tyr Tyr Met 
    1100                 1105                 1110             


Leu Asn  Leu Tyr Asp Pro Asn  Lys Tyr Val Asp Val  Asn Asn Val 
    1115                 1120                 1125             


Gly Ile  Arg Gly Tyr Met Tyr  Leu Lys Gly Pro Arg  Gly Ser Val 
    1130                 1135                 1140             


Met Thr  Thr Asn Ile Tyr Leu  Asn Ser Ser Leu Tyr  Arg Gly Thr 
    1145                 1150                 1155             


Lys Phe  Ile Ile Lys Lys Tyr  Ala Ser Gly Asn Lys  Asp Asn Ile 
    1160                 1165                 1170             


Val Arg  Asn Asn Asp Arg Val  Tyr Ile Asn Val Val  Val Lys Asn 
    1175                 1180                 1185             


Lys Glu  Tyr Arg Leu Ala Thr  Asn Ala Ser Gln Ala  Gly Val Glu 
    1190                 1195                 1200             


Lys Ile  Leu Ser Ala Leu Glu  Ile Pro Asp Val Gly  Asn Leu Ser 
    1205                 1210                 1215             


Gln Val  Val Val Met Lys Ser  Lys Asn Asp Gln Gly  Ile Thr Asn 
    1220                 1225                 1230             


Lys Cys  Lys Met Asn Leu Gln  Asp Asn Asn Gly Asn  Asp Ile Gly 
    1235                 1240                 1245             


Phe Ile  Gly Phe His Gln Phe  Asn Asn Ile Ala Lys  Leu Val Ala 
    1250                 1255                 1260             


Ser Asn  Trp Tyr Asn Arg Gln  Ile Glu Arg Ser Ser  Arg Thr Leu 
    1265                 1270                 1275             


Gly Cys  Ser Trp Glu Phe Ile  Pro Val Asp Asp Gly  Trp Gly Glu 
    1280                 1285                 1290             


Arg Pro  Leu 
    1295     


<210>  18
<211>  1291
<212>  PRT
<213>  Clostridium botulinum

<400>  18

Met Pro Val Thr Ile Asn Asn Phe Asn Tyr Asn Asp Pro Ile Asp Asn 
1               5                   10                  15      


Asn Asn Ile Ile Met Met Glu Pro Pro Phe Ala Arg Gly Thr Gly Arg 
            20                  25                  30          


Tyr Tyr Lys Ala Phe Lys Ile Thr Asp Arg Ile Trp Ile Ile Pro Glu 
        35                  40                  45              


Arg Tyr Thr Phe Gly Tyr Lys Pro Glu Asp Phe Asn Lys Ser Ser Gly 
    50                  55                  60                  


Ile Phe Asn Arg Asp Val Cys Glu Tyr Tyr Asp Pro Asp Tyr Leu Asn 
65                  70                  75                  80  


Thr Asn Asp Lys Lys Asn Ile Phe Leu Gln Thr Met Ile Lys Leu Phe 
                85                  90                  95      


Asn Arg Ile Lys Ser Lys Pro Leu Gly Glu Lys Leu Leu Glu Met Ile 
            100                 105                 110         


Ile Asn Gly Ile Pro Tyr Leu Gly Asp Arg Arg Val Pro Leu Glu Glu 
        115                 120                 125             


Phe Asn Thr Asn Ile Ala Ser Val Thr Val Asn Lys Leu Ile Ser Asn 
    130                 135                 140                 


Pro Gly Glu Val Glu Arg Lys Lys Gly Ile Phe Ala Asn Leu Ile Ile 
145                 150                 155                 160 


Phe Gly Pro Gly Pro Val Leu Asn Glu Asn Glu Thr Ile Asp Ile Gly 
                165                 170                 175     


Ile Gln Asn His Phe Ala Ser Arg Glu Gly Phe Gly Gly Ile Met Gln 
            180                 185                 190         


Met Lys Phe Cys Pro Glu Tyr Val Ser Val Phe Asn Asn Val Gln Glu 
        195                 200                 205             


Asn Lys Gly Ala Ser Ile Phe Asn Arg Arg Gly Tyr Phe Ser Asp Pro 
    210                 215                 220                 


Ala Leu Ile Leu Met His Glu Leu Ile His Val Leu His Gly Leu Tyr 
225                 230                 235                 240 


Gly Ile Lys Val Asp Asp Leu Pro Ile Val Pro Asn Glu Lys Lys Phe 
                245                 250                 255     


Phe Met Gln Ser Thr Asp Ala Ile Gln Ala Glu Glu Leu Tyr Thr Phe 
            260                 265                 270         


Gly Gly Gln Asp Pro Ser Ile Ile Thr Pro Ser Thr Asp Lys Ser Ile 
        275                 280                 285             


Tyr Asp Lys Val Leu Gln Asn Phe Arg Gly Ile Val Asp Arg Leu Asn 
    290                 295                 300                 


Lys Val Leu Val Cys Ile Ser Asp Pro Asn Ile Asn Ile Asn Ile Tyr 
305                 310                 315                 320 


Lys Asn Lys Phe Lys Asp Lys Tyr Lys Phe Val Glu Asp Ser Glu Gly 
                325                 330                 335     


Lys Tyr Ser Ile Asp Val Glu Ser Phe Asp Lys Leu Tyr Lys Ser Leu 
            340                 345                 350         


Met Phe Gly Phe Thr Glu Thr Asn Ile Ala Glu Asn Tyr Lys Ile Lys 
        355                 360                 365             


Thr Arg Ala Ser Tyr Phe Ser Asp Ser Leu Pro Pro Val Lys Ile Lys 
    370                 375                 380                 


Asn Leu Leu Asp Asn Glu Ile Tyr Thr Ile Glu Glu Gly Phe Asn Ile 
385                 390                 395                 400 


Ser Asp Lys Asp Met Glu Lys Glu Tyr Arg Gly Gln Asn Lys Ala Ile 
                405                 410                 415     


Asn Lys Gln Ala Tyr Glu Glu Ile Ser Lys Glu His Leu Ala Val Tyr 
            420                 425                 430         


Lys Ile Gln Met Cys Lys Ser Val Lys Ala Pro Gly Ile Cys Ile Asp 
        435                 440                 445             


Val Asp Asn Glu Asp Leu Phe Phe Ile Ala Asp Lys Asn Ser Phe Ser 
    450                 455                 460                 


Asp Asp Leu Ser Lys Asn Glu Arg Ile Glu Tyr Asn Thr Gln Ser Asn 
465                 470                 475                 480 


Tyr Ile Glu Asn Asp Phe Pro Ile Asn Glu Leu Ile Leu Asp Thr Asp 
                485                 490                 495     


Leu Ile Ser Lys Ile Glu Leu Pro Ser Glu Asn Thr Glu Ser Leu Thr 
            500                 505                 510         


Asp Phe Asn Val Asp Val Pro Val Tyr Glu Lys Gln Pro Ala Ile Lys 
        515                 520                 525             


Lys Ile Phe Thr Asp Glu Asn Thr Ile Phe Gln Tyr Leu Tyr Ser Gln 
    530                 535                 540                 


Thr Phe Pro Leu Asp Ile Arg Asp Ile Ser Leu Thr Ser Ser Phe Asp 
545                 550                 555                 560 


Asp Ala Leu Leu Phe Ser Asn Lys Val Tyr Ser Phe Phe Ser Met Asp 
                565                 570                 575     


Tyr Ile Lys Thr Ala Asn Lys Val Val Glu Ala Gly Leu Phe Ala Gly 
            580                 585                 590         


Trp Val Lys Gln Ile Val Asn Asp Phe Val Ile Glu Ala Asn Lys Ser 
        595                 600                 605             


Asn Thr Met Asp Lys Ile Ala Asp Ile Ser Leu Ile Val Pro Tyr Ile 
    610                 615                 620                 


Gly Leu Ala Leu Asn Val Gly Asn Glu Thr Ala Lys Gly Asn Phe Glu 
625                 630                 635                 640 


Asn Ala Phe Glu Ile Ala Gly Ala Ser Ile Leu Leu Glu Phe Ile Pro 
                645                 650                 655     


Glu Leu Leu Ile Pro Val Val Gly Ala Phe Leu Leu Glu Ser Tyr Ile 
            660                 665                 670         


Asp Asn Lys Asn Lys Ile Ile Lys Thr Ile Asp Asn Ala Leu Thr Lys 
        675                 680                 685             


Arg Asn Glu Lys Trp Ser Asp Met Tyr Gly Leu Ile Val Ala Gln Trp 
    690                 695                 700                 


Leu Ser Thr Val Asn Thr Gln Phe Tyr Thr Ile Lys Glu Gly Met Tyr 
705                 710                 715                 720 


Lys Ala Leu Asn Tyr Gln Ala Gln Ala Leu Glu Glu Ile Ile Lys Tyr 
                725                 730                 735     


Arg Tyr Asn Ile Tyr Ser Glu Lys Glu Lys Ser Asn Ile Asn Ile Asp 
            740                 745                 750         


Phe Asn Asp Ile Asn Ser Lys Leu Asn Glu Gly Ile Asn Gln Ala Ile 
        755                 760                 765             


Asp Asn Ile Asn Asn Phe Ile Asn Gly Cys Ser Val Ser Tyr Leu Met 
    770                 775                 780                 


Lys Lys Met Ile Pro Leu Ala Val Glu Lys Leu Leu Asp Phe Asp Asn 
785                 790                 795                 800 


Thr Leu Lys Lys Asn Leu Leu Asn Tyr Ile Asp Glu Asn Lys Leu Tyr 
                805                 810                 815     


Leu Ile Gly Ser Ala Glu Tyr Glu Lys Ser Lys Val Asn Lys Tyr Leu 
            820                 825                 830         


Lys Thr Ile Met Pro Phe Asp Leu Ser Ile Tyr Thr Asn Asp Thr Ile 
        835                 840                 845             


Leu Ile Glu Met Phe Asn Lys Tyr Asn Ser Glu Ile Leu Asn Asn Ile 
    850                 855                 860                 


Ile Leu Asn Leu Arg Tyr Lys Asp Asn Asn Leu Ile Asp Leu Ser Gly 
865                 870                 875                 880 


Tyr Gly Ala Lys Val Glu Val Tyr Asp Gly Val Glu Leu Asn Asp Lys 
                885                 890                 895     


Asn Gln Phe Lys Leu Thr Ser Ser Ala Asn Ser Lys Ile Arg Val Thr 
            900                 905                 910         


Gln Asn Gln Asn Ile Ile Phe Asn Ser Val Phe Leu Asp Phe Ser Val 
        915                 920                 925             


Ser Phe Trp Ile Arg Ile Pro Lys Tyr Lys Asn Asp Gly Ile Gln Asn 
    930                 935                 940                 


Tyr Ile His Asn Glu Tyr Thr Ile Ile Asn Cys Met Lys Asn Asn Ser 
945                 950                 955                 960 


Gly Trp Lys Ile Ser Ile Arg Gly Asn Arg Ile Ile Trp Thr Leu Ile 
                965                 970                 975     


Asp Ile Asn Gly Lys Thr Lys Ser Val Phe Phe Glu Tyr Asn Ile Arg 
            980                 985                 990         


Glu Asp Ile Ser Glu Tyr Ile Asn  Arg Trp Phe Phe Val  Thr Ile Thr 
        995                 1000                 1005             


Asn Asn  Leu Asn Asn Ala Lys  Ile Tyr Ile Asn Gly  Lys Leu Glu 
    1010                 1015                 1020             


Ser Asn  Thr Asp Ile Lys Asp  Ile Arg Glu Val Ile  Ala Asn Gly 
    1025                 1030                 1035             


Glu Ile  Ile Phe Lys Leu Asp  Gly Asp Ile Asp Arg  Thr Gln Phe 
    1040                 1045                 1050             


Ile Trp  Met Lys Tyr Phe Ser  Ile Phe Asn Thr Glu  Leu Ser Gln 
    1055                 1060                 1065             


Ser Asn  Ile Glu Glu Arg Tyr  Lys Ile Gln Ser Tyr  Ser Glu Tyr 
    1070                 1075                 1080             


Leu Lys  Asp Phe Trp Gly Asn  Pro Leu Met Tyr Asn  Lys Glu Tyr 
    1085                 1090                 1095             


Tyr Met  Phe Asn Ala Gly Asn  Lys Asn Ser Tyr Ile  Lys Leu Lys 
    1100                 1105                 1110             


Lys Asp  Ser Pro Val Gly Glu  Ile Leu Thr Arg Ser  Lys Tyr Asn 
    1115                 1120                 1125             


Gln Asn  Ser Lys Tyr Ile Asn  Tyr Arg Asp Leu Tyr  Ile Gly Glu 
    1130                 1135                 1140             


Lys Phe  Ile Ile Arg Arg Lys  Ser Asn Ser Gln Ser  Ile Asn Asp 
    1145                 1150                 1155             


Asp Ile  Val Arg Lys Glu Asp  Tyr Ile Tyr Leu Asp  Phe Phe Asn 
    1160                 1165                 1170             


Leu Asn  Gln Glu Trp Arg Val  Tyr Thr Tyr Lys Tyr  Phe Lys Lys 
    1175                 1180                 1185             


Glu Glu  Glu Lys Leu Phe Leu  Ala Pro Ile Ser Asp  Ser Asp Glu 
    1190                 1195                 1200             


Phe Tyr  Asn Thr Ile Gln Ile  Lys Glu Tyr Asp Glu  Gln Pro Thr 
    1205                 1210                 1215             


Tyr Ser  Cys Gln Leu Leu Phe  Lys Lys Asp Glu Glu  Ser Thr Asp 
    1220                 1225                 1230             


Glu Ile  Gly Leu Ile Gly Ile  His Arg Phe Tyr Glu  Ser Gly Ile 
    1235                 1240                 1245             


Val Phe  Glu Glu Tyr Lys Asp  Tyr Phe Cys Ile Ser  Lys Trp Tyr 
    1250                 1255                 1260             


Leu Lys  Glu Val Lys Arg Lys  Pro Tyr Asn Leu Lys  Leu Gly Cys 
    1265                 1270                 1275             


Asn Trp  Gln Phe Ile Pro Lys  Asp Glu Gly Trp Thr  Glu 
    1280                 1285                 1290     


<210>  19
<211>  1291
<212>  PRT
<213>  Clostridium botulinum

<400>  19

Met Pro Ile Thr Ile Asn Asn Phe Asn Tyr Ser Asp Pro Val Asp Asn 
1               5                   10                  15      


Lys Asn Ile Leu Tyr Leu Asp Thr His Leu Asn Thr Leu Ala Asn Glu 
            20                  25                  30          


Pro Glu Lys Ala Phe Arg Ile Thr Gly Asn Ile Trp Val Ile Pro Asp 
        35                  40                  45              


Arg Phe Ser Arg Asn Ser Asn Pro Asn Leu Asn Lys Pro Pro Arg Val 
    50                  55                  60                  


Thr Ser Pro Lys Ser Gly Tyr Tyr Asp Pro Asn Tyr Leu Ser Thr Asp 
65                  70                  75                  80  


Ser Asp Lys Asp Pro Phe Leu Lys Glu Ile Ile Lys Leu Phe Lys Arg 
                85                  90                  95      


Ile Asn Ser Arg Glu Ile Gly Glu Glu Leu Ile Tyr Arg Leu Ser Thr 
            100                 105                 110         


Asp Ile Pro Phe Pro Gly Asn Asn Asn Thr Pro Ile Asn Thr Phe Asp 
        115                 120                 125             


Phe Asp Val Asp Phe Asn Ser Val Asp Val Lys Thr Arg Gln Gly Asn 
    130                 135                 140                 


Asn Trp Val Lys Thr Gly Ser Ile Asn Pro Ser Val Ile Ile Thr Gly 
145                 150                 155                 160 


Pro Arg Glu Asn Ile Ile Asp Pro Glu Thr Ser Thr Phe Lys Leu Thr 
                165                 170                 175     


Asn Asn Thr Phe Ala Ala Gln Glu Gly Phe Gly Ala Leu Ser Ile Ile 
            180                 185                 190         


Ser Ile Ser Pro Arg Phe Met Leu Thr Tyr Ser Asn Ala Thr Asn Asp 
        195                 200                 205             


Val Gly Glu Gly Arg Phe Ser Lys Ser Glu Phe Cys Met Asp Pro Ile 
    210                 215                 220                 


Leu Ile Leu Met His Glu Leu Asn His Ala Met His Asn Leu Tyr Gly 
225                 230                 235                 240 


Ile Ala Ile Pro Asn Asp Gln Thr Ile Ser Ser Val Thr Ser Asn Ile 
                245                 250                 255     


Phe Tyr Ser Gln Tyr Asn Val Lys Leu Glu Tyr Ala Glu Ile Tyr Ala 
            260                 265                 270         


Phe Gly Gly Pro Thr Ile Asp Leu Ile Pro Lys Ser Ala Arg Lys Tyr 
        275                 280                 285             


Phe Glu Glu Lys Ala Leu Asp Tyr Tyr Arg Ser Ile Ala Lys Arg Leu 
    290                 295                 300                 


Asn Ser Ile Thr Thr Ala Asn Pro Ser Ser Phe Asn Lys Tyr Ile Gly 
305                 310                 315                 320 


Glu Tyr Lys Gln Lys Leu Ile Arg Lys Tyr Arg Phe Val Val Glu Ser 
                325                 330                 335     


Ser Gly Glu Val Thr Val Asn Arg Asn Lys Phe Val Glu Leu Tyr Asn 
            340                 345                 350         


Glu Leu Thr Gln Ile Phe Thr Glu Phe Asn Tyr Ala Lys Ile Tyr Asn 
        355                 360                 365             


Val Gln Asn Arg Lys Ile Tyr Leu Ser Asn Val Tyr Thr Pro Val Thr 
    370                 375                 380                 


Ala Asn Ile Leu Asp Asp Asn Val Tyr Asp Ile Gln Asn Gly Phe Asn 
385                 390                 395                 400 


Ile Pro Lys Ser Asn Leu Asn Val Leu Phe Met Gly Gln Asn Leu Ser 
                405                 410                 415     


Arg Asn Pro Ala Leu Arg Lys Val Asn Pro Glu Asn Met Leu Tyr Leu 
            420                 425                 430         


Phe Thr Lys Phe Cys His Lys Ala Ile Asp Gly Arg Ser Leu Tyr Asn 
        435                 440                 445             


Lys Thr Leu Asp Cys Arg Glu Leu Leu Val Lys Asn Thr Asp Leu Pro 
    450                 455                 460                 


Phe Ile Gly Asp Ile Ser Asp Val Lys Thr Asp Ile Phe Leu Arg Lys 
465                 470                 475                 480 


Asp Ile Asn Glu Glu Thr Glu Val Ile Tyr Tyr Pro Asp Asn Val Ser 
                485                 490                 495     


Val Asp Gln Val Ile Leu Ser Lys Asn Thr Ser Glu His Gly Gln Leu 
            500                 505                 510         


Asp Leu Leu Tyr Pro Ser Ile Asp Ser Glu Ser Glu Ile Leu Pro Gly 
        515                 520                 525             


Glu Asn Gln Val Phe Tyr Asp Asn Arg Thr Gln Asn Val Asp Tyr Leu 
    530                 535                 540                 


Asn Ser Tyr Tyr Tyr Leu Glu Ser Gln Lys Leu Ser Asp Asn Val Glu 
545                 550                 555                 560 


Asp Phe Thr Phe Thr Arg Ser Ile Glu Glu Ala Leu Asp Asn Ser Ala 
                565                 570                 575     


Lys Val Tyr Thr Tyr Phe Pro Thr Leu Ala Asn Lys Val Asn Ala Gly 
            580                 585                 590         


Val Gln Gly Gly Leu Phe Leu Met Trp Ala Asn Asp Val Val Glu Asp 
        595                 600                 605             


Phe Thr Thr Asn Ile Leu Arg Lys Asp Thr Leu Asp Lys Ile Ser Asp 
    610                 615                 620                 


Val Ser Ala Ile Ile Pro Tyr Ile Gly Pro Ala Leu Asn Ile Ser Asn 
625                 630                 635                 640 


Ser Val Arg Arg Gly Asn Phe Thr Glu Ala Phe Ala Val Thr Gly Val 
                645                 650                 655     


Thr Ile Leu Leu Glu Ala Phe Pro Glu Phe Thr Ile Pro Ala Leu Gly 
            660                 665                 670         


Ala Phe Val Ile Tyr Ser Lys Val Gln Glu Arg Asn Glu Ile Ile Lys 
        675                 680                 685             


Thr Ile Asp Asn Cys Leu Glu Gln Arg Ile Lys Arg Trp Lys Asp Ser 
    690                 695                 700                 


Tyr Glu Trp Met Met Gly Thr Trp Leu Ser Arg Ile Ile Thr Gln Phe 
705                 710                 715                 720 


Asn Asn Ile Ser Tyr Gln Met Tyr Asp Ser Leu Asn Tyr Gln Ala Gly 
                725                 730                 735     


Ala Ile Lys Ala Lys Ile Asp Leu Glu Tyr Lys Lys Tyr Ser Gly Ser 
            740                 745                 750         


Asp Lys Glu Asn Ile Lys Ser Gln Val Glu Asn Leu Lys Asn Ser Leu 
        755                 760                 765             


Asp Val Lys Ile Ser Glu Ala Met Asn Asn Ile Asn Lys Phe Ile Arg 
    770                 775                 780                 


Glu Cys Ser Val Thr Tyr Leu Phe Lys Asn Met Leu Pro Lys Val Ile 
785                 790                 795                 800 


Asp Glu Leu Asn Glu Phe Asp Arg Asn Thr Lys Ala Lys Leu Ile Asn 
                805                 810                 815     


Leu Ile Asp Ser His Asn Ile Ile Leu Val Gly Glu Val Asp Lys Leu 
            820                 825                 830         


Lys Ala Lys Val Asn Asn Ser Phe Gln Asn Thr Ile Pro Phe Asn Ile 
        835                 840                 845             


Phe Ser Tyr Thr Asn Asn Ser Leu Leu Lys Asp Ile Ile Asn Glu Tyr 
    850                 855                 860                 


Phe Asn Asn Ile Asn Asp Ser Lys Ile Leu Ser Leu Gln Asn Arg Lys 
865                 870                 875                 880 


Asn Thr Leu Val Asp Thr Ser Gly Tyr Asn Ala Glu Val Ser Glu Glu 
                885                 890                 895     


Gly Asp Val Gln Leu Asn Pro Ile Phe Pro Phe Asp Phe Lys Leu Gly 
            900                 905                 910         


Ser Ser Gly Glu Asp Arg Gly Lys Val Ile Val Thr Gln Asn Glu Asn 
        915                 920                 925             


Ile Val Tyr Asn Ser Met Tyr Glu Ser Phe Ser Ile Ser Phe Trp Ile 
    930                 935                 940                 


Arg Ile Asn Lys Trp Val Ser Asn Leu Pro Gly Tyr Thr Ile Ile Asp 
945                 950                 955                 960 


Ser Val Lys Asn Asn Ser Gly Trp Ser Ile Gly Ile Ile Ser Asn Phe 
                965                 970                 975     


Leu Val Phe Thr Leu Lys Gln Asn Glu Asp Ser Glu Gln Ser Ile Asn 
            980                 985                 990         


Phe Ser Tyr Asp Ile Ser Asn Asn  Ala Pro Gly Tyr Asn  Lys Trp Phe 
        995                 1000                 1005             


Phe Val  Thr Val Thr Asn Asn  Met Met Gly Asn Met  Lys Ile Tyr 
    1010                 1015                 1020             


Ile Asn  Gly Lys Leu Ile Asp  Thr Ile Lys Val Lys  Glu Leu Thr 
    1025                 1030                 1035             


Gly Ile  Asn Phe Ser Lys Thr  Ile Thr Phe Glu Ile  Asn Lys Ile 
    1040                 1045                 1050             


Pro Asp  Thr Gly Leu Ile Thr  Ser Asp Ser Asp Asn  Ile Asn Met 
    1055                 1060                 1065             


Trp Ile  Arg Asp Phe Tyr Ile  Phe Ala Lys Glu Leu  Asp Gly Lys 
    1070                 1075                 1080             


Asp Ile  Asn Ile Leu Phe Asn  Ser Leu Gln Tyr Thr  Asn Val Val 
    1085                 1090                 1095             


Lys Asp  Tyr Trp Gly Asn Asp  Leu Arg Tyr Asn Lys  Glu Tyr Tyr 
    1100                 1105                 1110             


Met Val  Asn Ile Asp Tyr Leu  Asn Arg Tyr Met Tyr  Ala Asn Ser 
    1115                 1120                 1125             


Arg Gln  Ile Val Phe Asn Thr  Arg Arg Asn Asn Asn  Asp Phe Asn 
    1130                 1135                 1140             


Glu Gly  Tyr Lys Ile Ile Ile  Lys Arg Ile Arg Gly  Asn Thr Asn 
    1145                 1150                 1155             


Asp Thr  Arg Val Arg Gly Gly  Asp Ile Leu Tyr Phe  Asp Met Thr 
    1160                 1165                 1170             


Ile Asn  Asn Lys Ala Tyr Asn  Leu Phe Met Lys Asn  Glu Thr Met 
    1175                 1180                 1185             


Tyr Ala  Asp Asn His Ser Thr  Glu Asp Ile Tyr Ala  Ile Gly Leu 
    1190                 1195                 1200             


Arg Glu  Gln Thr Lys Asp Ile  Asn Asp Asn Ile Ile  Phe Gln Ile 
    1205                 1210                 1215             


Gln Pro  Met Asn Asn Thr Tyr  Tyr Tyr Ala Ser Gln  Ile Phe Lys 
    1220                 1225                 1230             


Ser Asn  Phe Asn Gly Glu Asn  Ile Ser Gly Ile Cys  Ser Ile Gly 
    1235                 1240                 1245             


Thr Tyr  Arg Phe Arg Leu Gly  Gly Asp Trp Tyr Arg  His Asn Tyr 
    1250                 1255                 1260             


Leu Val  Pro Thr Val Lys Gln  Gly Asn Tyr Ala Ser  Leu Leu Glu 
    1265                 1270                 1275             


Ser Thr  Ser Thr His Trp Gly  Phe Val Pro Val Ser  Glu 
    1280                 1285                 1290     


<210>  20
<211>  1276
<212>  PRT
<213>  Clostridium botulinum

<400>  20

Met Thr Trp Pro Val Lys Asp Phe Asn Tyr Ser Asp Pro Val Asn Asp 
1               5                   10                  15      


Asn Asp Ile Leu Tyr Leu Arg Ile Pro Gln Asn Lys Leu Ile Thr Thr 
            20                  25                  30          


Pro Val Lys Ala Phe Met Ile Thr Gln Asn Ile Trp Val Ile Pro Glu 
        35                  40                  45              


Arg Phe Ser Ser Asp Thr Asn Pro Ser Leu Ser Lys Pro Pro Arg Pro 
    50                  55                  60                  


Thr Ser Lys Tyr Gln Ser Tyr Tyr Asp Pro Ser Tyr Leu Ser Thr Asp 
65                  70                  75                  80  


Glu Gln Lys Asp Thr Phe Leu Lys Gly Ile Ile Lys Leu Phe Lys Arg 
                85                  90                  95      


Ile Asn Glu Arg Asp Ile Gly Lys Lys Leu Ile Asn Tyr Leu Val Val 
            100                 105                 110         


Gly Ser Pro Phe Met Gly Asp Ser Ser Thr Pro Glu Asp Thr Phe Asp 
        115                 120                 125             


Phe Thr Arg His Thr Thr Asn Ile Ala Val Glu Lys Phe Glu Asn Gly 
    130                 135                 140                 


Ser Trp Lys Val Thr Asn Ile Ile Thr Pro Ser Val Leu Ile Phe Gly 
145                 150                 155                 160 


Pro Leu Pro Asn Ile Leu Asp Tyr Thr Ala Ser Leu Thr Leu Gln Gly 
                165                 170                 175     


Gln Gln Ser Asn Pro Ser Phe Glu Gly Phe Gly Thr Leu Ser Ile Leu 
            180                 185                 190         


Lys Val Ala Pro Glu Phe Leu Leu Thr Phe Ser Asp Val Thr Ser Asn 
        195                 200                 205             


Gln Ser Ser Ala Val Leu Gly Lys Ser Ile Phe Cys Met Asp Pro Val 
    210                 215                 220                 


Ile Ala Leu Met His Glu Leu Thr His Ser Leu His Gln Leu Tyr Gly 
225                 230                 235                 240 


Ile Asn Ile Pro Ser Asp Lys Arg Ile Arg Pro Gln Val Ser Glu Gly 
                245                 250                 255     


Phe Phe Ser Gln Asp Gly Pro Asn Val Gln Phe Glu Glu Leu Tyr Thr 
            260                 265                 270         


Phe Gly Gly Leu Asp Val Glu Ile Ile Pro Gln Ile Glu Arg Ser Gln 
        275                 280                 285             


Leu Arg Glu Lys Ala Leu Gly His Tyr Lys Asp Ile Ala Lys Arg Leu 
    290                 295                 300                 


Asn Asn Ile Asn Lys Thr Ile Pro Ser Ser Trp Ile Ser Asn Ile Asp 
305                 310                 315                 320 


Lys Tyr Lys Lys Ile Phe Ser Glu Lys Tyr Asn Phe Asp Lys Asp Asn 
                325                 330                 335     


Thr Gly Asn Phe Val Val Asn Ile Asp Lys Phe Asn Ser Leu Tyr Ser 
            340                 345                 350         


Asp Leu Thr Asn Val Met Ser Glu Val Val Tyr Ser Ser Gln Tyr Asn 
        355                 360                 365             


Val Lys Asn Arg Thr His Tyr Phe Ser Arg His Tyr Leu Pro Val Phe 
    370                 375                 380                 


Ala Asn Ile Leu Asp Asp Asn Ile Tyr Thr Ile Arg Asp Gly Phe Asn 
385                 390                 395                 400 


Leu Thr Asn Lys Gly Phe Asn Ile Glu Asn Ser Gly Gln Asn Ile Glu 
                405                 410                 415     


Arg Asn Pro Ala Leu Gln Lys Leu Ser Ser Glu Ser Val Val Asp Leu 
            420                 425                 430         


Phe Thr Lys Val Cys Leu Arg Leu Thr Lys Asn Ser Arg Asp Asp Ser 
        435                 440                 445             


Thr Cys Ile Lys Val Lys Asn Asn Arg Leu Pro Tyr Val Ala Asp Lys 
    450                 455                 460                 


Asp Ser Ile Ser Gln Glu Ile Phe Glu Asn Lys Ile Ile Thr Asp Glu 
465                 470                 475                 480 


Thr Asn Val Gln Asn Tyr Ser Asp Lys Phe Ser Leu Asp Glu Ser Ile 
                485                 490                 495     


Leu Asp Gly Gln Val Pro Ile Asn Pro Glu Ile Val Asp Pro Leu Leu 
            500                 505                 510         


Pro Asn Val Asn Met Glu Pro Leu Asn Leu Pro Gly Glu Glu Ile Val 
        515                 520                 525             


Phe Tyr Asp Asp Ile Thr Lys Tyr Val Asp Tyr Leu Asn Ser Tyr Tyr 
    530                 535                 540                 


Tyr Leu Glu Ser Gln Lys Leu Ser Asn Asn Val Glu Asn Ile Thr Leu 
545                 550                 555                 560 


Thr Thr Ser Val Glu Glu Ala Leu Gly Tyr Ser Asn Lys Ile Tyr Thr 
                565                 570                 575     


Phe Leu Pro Ser Leu Ala Glu Lys Val Asn Lys Gly Val Gln Ala Gly 
            580                 585                 590         


Leu Phe Leu Asn Trp Ala Asn Glu Val Val Glu Asp Phe Thr Thr Asn 
        595                 600                 605             


Ile Met Lys Lys Asp Thr Leu Asp Lys Ile Ser Asp Val Ser Val Ile 
    610                 615                 620                 


Ile Pro Tyr Ile Gly Pro Ala Leu Asn Ile Gly Asn Ser Ala Leu Arg 
625                 630                 635                 640 


Gly Asn Phe Asn Gln Ala Phe Ala Thr Ala Gly Val Ala Phe Leu Leu 
                645                 650                 655     


Glu Gly Phe Pro Glu Phe Thr Ile Pro Ala Leu Gly Val Phe Thr Phe 
            660                 665                 670         


Tyr Ser Ser Ile Gln Glu Arg Glu Lys Ile Ile Lys Thr Ile Glu Asn 
        675                 680                 685             


Cys Leu Glu Gln Arg Val Lys Arg Trp Lys Asp Ser Tyr Gln Trp Met 
    690                 695                 700                 


Val Ser Asn Trp Leu Ser Arg Ile Thr Thr Gln Phe Asn His Ile Asn 
705                 710                 715                 720 


Tyr Gln Met Tyr Asp Ser Leu Ser Tyr Gln Ala Asp Ala Ile Lys Ala 
                725                 730                 735     


Lys Ile Asp Leu Glu Tyr Lys Lys Tyr Ser Gly Ser Asp Lys Glu Asn 
            740                 745                 750         


Ile Lys Ser Gln Val Glu Asn Leu Lys Asn Ser Leu Asp Val Lys Ile 
        755                 760                 765             


Ser Glu Ala Met Asn Asn Ile Asn Lys Phe Ile Arg Glu Cys Ser Val 
    770                 775                 780                 


Thr Tyr Leu Phe Lys Asn Met Leu Pro Lys Val Ile Asp Glu Leu Asn 
785                 790                 795                 800 


Lys Phe Asp Leu Arg Thr Lys Thr Glu Leu Ile Asn Leu Ile Asp Ser 
                805                 810                 815     


His Asn Ile Ile Leu Val Gly Glu Val Asp Arg Leu Lys Ala Lys Val 
            820                 825                 830         


Asn Glu Ser Phe Glu Asn Thr Met Pro Phe Asn Ile Phe Ser Tyr Thr 
        835                 840                 845             


Asn Asn Ser Leu Leu Lys Asp Ile Ile Asn Glu Tyr Phe Asn Ser Ile 
    850                 855                 860                 


Asn Asp Ser Lys Ile Leu Ser Leu Gln Asn Lys Lys Asn Ala Leu Val 
865                 870                 875                 880 


Asp Thr Ser Gly Tyr Asn Ala Glu Val Arg Val Gly Asp Asn Val Gln 
                885                 890                 895     


Leu Asn Thr Ile Tyr Thr Asn Asp Phe Lys Leu Ser Ser Ser Gly Asp 
            900                 905                 910         


Lys Ile Ile Val Asn Leu Asn Asn Asn Ile Leu Tyr Ser Ala Ile Tyr 
        915                 920                 925             


Glu Asn Ser Ser Val Ser Phe Trp Ile Lys Ile Ser Lys Asp Leu Thr 
    930                 935                 940                 


Asn Ser His Asn Glu Tyr Thr Ile Ile Asn Ser Ile Glu Gln Asn Ser 
945                 950                 955                 960 


Gly Trp Lys Leu Cys Ile Arg Asn Gly Asn Ile Glu Trp Ile Leu Gln 
                965                 970                 975     


Asp Val Asn Arg Lys Tyr Lys Ser Leu Ile Phe Asp Tyr Ser Glu Ser 
            980                 985                 990         


Leu Ser His Thr Gly Tyr Thr Asn  Lys Trp Phe Phe Val  Thr Ile Thr 
        995                 1000                 1005             


Asn Asn  Ile Met Gly Tyr Met  Lys Leu Tyr Ile Asn  Gly Glu Leu 
    1010                 1015                 1020             


Lys Gln  Ser Gln Lys Ile Glu  Asp Leu Asp Glu Val  Lys Leu Asp 
    1025                 1030                 1035             


Lys Thr  Ile Val Phe Gly Ile  Asp Glu Asn Ile Asp  Glu Asn Gln 
    1040                 1045                 1050             


Met Leu  Trp Ile Arg Asp Phe  Asn Ile Phe Ser Lys  Glu Leu Ser 
    1055                 1060                 1065             


Asn Glu  Asp Ile Asn Ile Val  Tyr Glu Gly Gln Ile  Leu Arg Asn 
    1070                 1075                 1080             


Val Ile  Lys Asp Tyr Trp Gly  Asn Pro Leu Lys Phe  Asp Thr Glu 
    1085                 1090                 1095             


Tyr Tyr  Ile Ile Asn Asp Asn  Tyr Ile Asp Arg Tyr  Ile Ala Pro 
    1100                 1105                 1110             


Glu Ser  Asn Val Leu Val Leu  Val Gln Tyr Pro Asp  Arg Ser Lys 
    1115                 1120                 1125             


Leu Tyr  Thr Gly Asn Pro Ile  Thr Ile Lys Ser Val  Ser Asp Lys 
    1130                 1135                 1140             


Asn Pro  Tyr Ser Arg Ile Leu  Asn Gly Asp Asn Ile  Ile Leu His 
    1145                 1150                 1155             


Met Leu  Tyr Asn Ser Arg Lys  Tyr Met Ile Ile Arg  Asp Thr Asp 
    1160                 1165                 1170             


Thr Ile  Tyr Ala Thr Gln Gly  Gly Glu Cys Ser Gln  Asn Cys Val 
    1175                 1180                 1185             


Tyr Ala  Leu Lys Leu Gln Ser  Asn Leu Gly Asn Tyr  Gly Ile Gly 
    1190                 1195                 1200             


Ile Phe  Ser Ile Lys Asn Ile  Val Ser Lys Asn Lys  Tyr Cys Ser 
    1205                 1210                 1215             


Gln Ile  Phe Ser Ser Phe Arg  Glu Asn Thr Met Leu  Leu Ala Asp 
    1220                 1225                 1230             


Ile Tyr  Lys Pro Trp Arg Phe  Ser Phe Lys Asn Ala  Tyr Thr Pro 
    1235                 1240                 1245             


Val Ala  Val Thr Asn Tyr Glu  Thr Lys Leu Leu Ser  Thr Ser Ser 
    1250                 1255                 1260             


Phe Trp  Lys Phe Ile Ser Arg  Asp Pro Gly Trp Val  Glu 
    1265                 1270                 1275     


<210>  21
<211>  1251
<212>  PRT
<213>  Clostridium botulinum

<400>  21

Met Pro Lys Ile Asn Ser Phe Asn Tyr Asn Asp Pro Val Asn Asp Arg 
1               5                   10                  15      


Thr Ile Leu Tyr Ile Lys Pro Gly Gly Cys Gln Glu Phe Tyr Lys Ser 
            20                  25                  30          


Phe Asn Ile Met Lys Asn Ile Trp Ile Ile Pro Glu Arg Asn Val Ile 
        35                  40                  45              


Gly Thr Thr Pro Gln Asp Phe His Pro Pro Thr Ser Leu Lys Asn Gly 
    50                  55                  60                  


Asp Ser Ser Tyr Tyr Asp Pro Asn Tyr Leu Gln Ser Asp Glu Glu Lys 
65                  70                  75                  80  


Asp Arg Phe Leu Lys Ile Val Thr Lys Ile Phe Asn Arg Ile Asn Asn 
                85                  90                  95      


Asn Leu Ser Gly Gly Ile Leu Leu Glu Glu Leu Ser Lys Ala Asn Pro 
            100                 105                 110         


Tyr Leu Gly Asn Asp Asn Thr Pro Asp Asn Gln Phe His Ile Gly Asp 
        115                 120                 125             


Ala Ser Ala Val Glu Ile Lys Phe Ser Asn Gly Ser Gln Asp Ile Leu 
    130                 135                 140                 


Leu Pro Asn Val Ile Ile Met Gly Ala Glu Pro Asp Leu Phe Glu Thr 
145                 150                 155                 160 


Asn Ser Ser Asn Ile Ser Leu Arg Asn Asn Tyr Met Pro Ser Asn His 
                165                 170                 175     


Arg Phe Gly Ser Ile Ala Ile Val Thr Phe Ser Pro Glu Tyr Ser Phe 
            180                 185                 190         


Arg Phe Asn Asp Asn Cys Met Asn Glu Phe Ile Gln Asp Pro Ala Leu 
        195                 200                 205             


Thr Leu Met His Glu Leu Ile His Ser Leu His Gly Leu Tyr Gly Ala 
    210                 215                 220                 


Lys Gly Ile Thr Thr Lys Tyr Thr Ile Thr Gln Lys Gln Asn Pro Leu 
225                 230                 235                 240 


Ile Thr Asn Ile Arg Gly Thr Asn Ile Glu Glu Phe Leu Thr Phe Gly 
                245                 250                 255     


Gly Thr Asp Leu Asn Ile Ile Thr Ser Ala Gln Ser Asn Asp Ile Tyr 
            260                 265                 270         


Thr Asn Leu Leu Ala Asp Tyr Lys Lys Ile Ala Ser Lys Leu Ser Lys 
        275                 280                 285             


Val Gln Val Ser Asn Pro Leu Leu Asn Pro Tyr Lys Asp Val Phe Glu 
    290                 295                 300                 


Ala Lys Tyr Gly Leu Asp Lys Asp Ala Ser Gly Ile Tyr Ser Val Asn 
305                 310                 315                 320 


Ile Asn Lys Phe Asn Asp Ile Phe Lys Lys Leu Tyr Ser Phe Thr Glu 
                325                 330                 335     


Phe Asp Leu Arg Thr Lys Phe Gln Val Lys Cys Arg Gln Thr Tyr Ile 
            340                 345                 350         


Gly Gln Tyr Lys Tyr Phe Lys Leu Ser Asn Leu Leu Asn Asp Ser Ile 
        355                 360                 365             


Tyr Asn Ile Ser Glu Gly Tyr Asn Ile Asn Asn Leu Lys Val Asn Phe 
    370                 375                 380                 


Arg Gly Gln Asn Ala Asn Leu Asn Pro Arg Ile Ile Thr Pro Ile Thr 
385                 390                 395                 400 


Gly Arg Gly Leu Val Lys Lys Ile Ile Arg Phe Cys Lys Asn Ile Val 
                405                 410                 415     


Ser Val Lys Gly Ile Arg Lys Ser Ile Cys Ile Glu Ile Asn Asn Gly 
            420                 425                 430         


Glu Leu Phe Phe Val Ala Ser Glu Asn Ser Tyr Asn Asp Asp Asn Ile 
        435                 440                 445             


Asn Thr Pro Lys Glu Ile Asp Asp Thr Val Thr Ser Asn Asn Asn Tyr 
    450                 455                 460                 


Glu Asn Asp Leu Asp Gln Val Ile Leu Asn Phe Asn Ser Glu Ser Ala 
465                 470                 475                 480 


Pro Gly Leu Ser Asp Glu Lys Leu Asn Leu Thr Ile Gln Asn Asp Ala 
                485                 490                 495     


Tyr Ile Pro Lys Tyr Asp Ser Asn Gly Thr Ser Asp Ile Glu Gln His 
            500                 505                 510         


Asp Val Asn Glu Leu Asn Val Phe Phe Tyr Leu Asp Ala Gln Lys Val 
        515                 520                 525             


Pro Glu Gly Glu Asn Asn Val Asn Leu Thr Ser Ser Ile Asp Thr Ala 
    530                 535                 540                 


Leu Leu Glu Gln Pro Lys Ile Tyr Thr Phe Phe Ser Ser Glu Phe Ile 
545                 550                 555                 560 


Asn Asn Val Asn Lys Pro Val Gln Ala Ala Leu Phe Val Ser Trp Ile 
                565                 570                 575     


Gln Gln Val Leu Val Asp Phe Thr Thr Glu Ala Asn Gln Lys Ser Thr 
            580                 585                 590         


Val Asp Lys Ile Ala Asp Ile Ser Ile Val Val Pro Tyr Ile Gly Leu 
        595                 600                 605             


Ala Leu Asn Ile Gly Asn Glu Ala Gln Lys Gly Asn Phe Lys Asp Ala 
    610                 615                 620                 


Leu Glu Leu Leu Gly Ala Gly Ile Leu Leu Glu Phe Glu Pro Glu Leu 
625                 630                 635                 640 


Leu Ile Pro Thr Ile Leu Val Phe Thr Ile Lys Ser Phe Leu Gly Ser 
                645                 650                 655     


Ser Asp Asn Lys Asn Lys Val Ile Lys Ala Ile Asn Asn Ala Leu Lys 
            660                 665                 670         


Glu Arg Asp Glu Lys Trp Lys Glu Val Tyr Ser Phe Ile Val Ser Asn 
        675                 680                 685             


Trp Met Thr Lys Ile Asn Thr Gln Phe Asn Lys Arg Lys Glu Gln Met 
    690                 695                 700                 


Tyr Gln Ala Leu Gln Asn Gln Val Asn Ala Ile Lys Thr Ile Ile Glu 
705                 710                 715                 720 


Ser Lys Tyr Asn Ser Tyr Thr Leu Glu Glu Lys Asn Glu Leu Thr Asn 
                725                 730                 735     


Lys Tyr Asp Ile Lys Gln Ile Glu Asn Glu Leu Asn Gln Lys Val Ser 
            740                 745                 750         


Ile Ala Met Asn Asn Ile Asp Arg Phe Leu Thr Glu Ser Ser Ile Ser 
        755                 760                 765             


Tyr Leu Met Lys Ile Ile Asn Glu Val Lys Ile Asn Lys Leu Arg Glu 
    770                 775                 780                 


Tyr Asp Glu Asn Val Lys Thr Tyr Leu Leu Asn Tyr Ile Ile Gln His 
785                 790                 795                 800 


Gly Ser Ile Leu Gly Glu Ser Gln Gln Glu Leu Asn Ser Met Val Thr 
                805                 810                 815     


Asp Thr Leu Asn Asn Ser Ile Pro Phe Lys Leu Ser Ser Tyr Thr Asp 
            820                 825                 830         


Asp Lys Ile Leu Ile Ser Tyr Phe Asn Lys Phe Phe Lys Arg Ile Lys 
        835                 840                 845             


Ser Ser Ser Val Leu Asn Met Arg Tyr Lys Asn Asp Lys Tyr Val Asp 
    850                 855                 860                 


Thr Ser Gly Tyr Asp Ser Asn Ile Asn Ile Asn Gly Asp Val Tyr Lys 
865                 870                 875                 880 


Tyr Pro Thr Asn Lys Asn Gln Phe Gly Ile Tyr Asn Asp Lys Leu Ser 
                885                 890                 895     


Glu Val Asn Ile Ser Gln Asn Asp Tyr Ile Ile Tyr Asp Asn Lys Tyr 
            900                 905                 910         


Lys Asn Phe Ser Ile Ser Phe Trp Val Arg Ile Pro Asn Tyr Asp Asn 
        915                 920                 925             


Lys Ile Val Asn Val Asn Asn Glu Tyr Thr Ile Ile Asn Cys Met Arg 
    930                 935                 940                 


Asp Asn Asn Ser Gly Trp Lys Val Ser Leu Asn His Asn Glu Ile Ile 
945                 950                 955                 960 


Trp Thr Phe Glu Asp Asn Arg Gly Ile Asn Gln Lys Leu Ala Phe Asn 
                965                 970                 975     


Tyr Gly Asn Ala Asn Gly Ile Ser Asp Tyr Ile Asn Lys Trp Ile Phe 
            980                 985                 990         


Val Thr Ile Thr Asn Asp Arg Leu  Gly Asp Ser Lys Leu  Tyr Ile Asn 
        995                 1000                 1005             


Gly Asn  Leu Ile Asp Gln Lys  Ser Ile Leu Asn Leu  Gly Asn Ile 
    1010                 1015                 1020             


His Val  Ser Asp Asn Ile Leu  Phe Lys Ile Val Asn  Cys Ser Tyr 
    1025                 1030                 1035             


Thr Arg  Tyr Ile Gly Ile Arg  Tyr Phe Asn Ile Phe  Asp Lys Glu 
    1040                 1045                 1050             


Leu Asp  Glu Thr Glu Ile Gln  Thr Leu Tyr Ser Asn  Glu Pro Asn 
    1055                 1060                 1065             


Thr Asn  Ile Leu Lys Asp Phe  Trp Gly Asn Tyr Leu  Leu Tyr Asp 
    1070                 1075                 1080             


Lys Glu  Tyr Tyr Leu Leu Asn  Val Leu Lys Pro Asn  Asn Phe Ile 
    1085                 1090                 1095             


Asp Arg  Arg Lys Asp Ser Thr  Leu Ser Ile Asn Asn  Ile Arg Ser 
    1100                 1105                 1110             


Thr Ile  Leu Leu Ala Asn Arg  Leu Tyr Ser Gly Ile  Lys Val Lys 
    1115                 1120                 1125             


Ile Gln  Arg Val Asn Asn Ser  Ser Thr Asn Asp Asn  Leu Val Arg 
    1130                 1135                 1140             


Lys Asn  Asp Gln Val Tyr Ile  Asn Phe Val Ala Ser  Lys Thr His 
    1145                 1150                 1155             


Leu Phe  Pro Leu Tyr Ala Asp  Thr Ala Thr Thr Asn  Lys Glu Lys 
    1160                 1165                 1170             


Thr Ile  Lys Ile Ser Ser Ser  Gly Asn Arg Phe Asn  Gln Val Val 
    1175                 1180                 1185             


Val Met  Asn Ser Val Gly Asn  Cys Thr Met Asn Phe  Lys Asn Asn 
    1190                 1195                 1200             


Asn Gly  Asn Asn Ile Gly Leu  Leu Gly Phe Lys Ala  Asp Thr Val 
    1205                 1210                 1215             


Val Ala  Ser Thr Trp Tyr Tyr  Thr His Met Arg Asp  His Thr Asn 
    1220                 1225                 1230             


Ser Asn  Gly Cys Phe Trp Asn  Phe Ile Ser Glu Glu  His Gly Trp 
    1235                 1240                 1245             


Gln Glu  Lys 
    1250     


<210>  22
<211>  1278
<212>  PRT
<213>  Clostridium botulinum

<400>  22

Met Pro Val Val Ile Asn Ser Phe Asn Tyr Asn Asp Pro Val Asn Asp 
1               5                   10                  15      


Asp Thr Ile Leu Tyr Met Gln Ile Pro Tyr Glu Glu Lys Ser Lys Lys 
            20                  25                  30          


Tyr Tyr Lys Ala Phe Glu Ile Met Arg Asn Val Trp Ile Ile Pro Glu 
        35                  40                  45              


Arg Asn Thr Ile Gly Thr Asp Pro Ser Asp Phe Asp Pro Pro Ala Ser 
    50                  55                  60                  


Leu Glu Asn Gly Ser Ser Ala Tyr Tyr Asp Pro Asn Tyr Leu Thr Thr 
65                  70                  75                  80  


Asp Ala Glu Lys Asp Arg Tyr Leu Lys Thr Thr Ile Lys Leu Phe Lys 
                85                  90                  95      


Arg Ile Asn Ser Asn Pro Ala Gly Glu Val Leu Leu Gln Glu Ile Ser 
            100                 105                 110         


Tyr Ala Lys Pro Tyr Leu Gly Asn Glu His Thr Pro Ile Asn Glu Phe 
        115                 120                 125             


His Pro Val Thr Arg Thr Thr Ser Val Asn Ile Lys Ser Ser Thr Asn 
    130                 135                 140                 


Val Lys Ser Ser Ile Ile Leu Asn Leu Leu Val Leu Gly Ala Gly Pro 
145                 150                 155                 160 


Asp Ile Phe Glu Asn Ser Ser Tyr Pro Val Arg Lys Leu Met Asp Ser 
                165                 170                 175     


Gly Gly Val Tyr Asp Pro Ser Asn Asp Gly Phe Gly Ser Ile Asn Ile 
            180                 185                 190         


Val Thr Phe Ser Pro Glu Tyr Glu Tyr Thr Phe Asn Asp Ile Ser Gly 
        195                 200                 205             


Gly Tyr Asn Ser Ser Thr Glu Ser Phe Ile Ala Asp Pro Ala Ile Ser 
    210                 215                 220                 


Leu Ala His Glu Leu Ile His Ala Leu His Gly Leu Tyr Gly Ala Arg 
225                 230                 235                 240 


Gly Val Thr Tyr Lys Glu Thr Ile Lys Val Lys Gln Ala Pro Leu Met 
                245                 250                 255     


Ile Ala Glu Lys Pro Ile Arg Leu Glu Glu Phe Leu Thr Phe Gly Gly 
            260                 265                 270         


Gln Asp Leu Asn Ile Ile Thr Ser Ala Met Lys Glu Lys Ile Tyr Asn 
        275                 280                 285             


Asn Leu Leu Ala Asn Tyr Glu Lys Ile Ala Thr Arg Leu Ser Arg Val 
    290                 295                 300                 


Asn Ser Ala Pro Pro Glu Tyr Asp Ile Asn Glu Tyr Lys Asp Tyr Phe 
305                 310                 315                 320 


Gln Trp Lys Tyr Gly Leu Asp Lys Asn Ala Asp Gly Ser Tyr Thr Val 
                325                 330                 335     


Asn Glu Asn Lys Phe Asn Glu Ile Tyr Lys Lys Leu Tyr Ser Phe Thr 
            340                 345                 350         


Glu Ile Asp Leu Ala Asn Lys Phe Lys Val Lys Cys Arg Asn Thr Tyr 
        355                 360                 365             


Phe Ile Lys Tyr Gly Phe Leu Lys Val Pro Asn Leu Leu Asp Asp Asp 
    370                 375                 380                 


Ile Tyr Thr Val Ser Glu Gly Phe Asn Ile Gly Asn Leu Ala Val Asn 
385                 390                 395                 400 


Asn Arg Gly Gln Asn Ile Lys Leu Asn Pro Lys Ile Ile Asp Ser Ile 
                405                 410                 415     


Pro Asp Lys Gly Leu Val Glu Lys Ile Val Lys Phe Cys Lys Ser Val 
            420                 425                 430         


Ile Pro Arg Lys Gly Thr Lys Ala Pro Pro Arg Leu Cys Ile Arg Val 
        435                 440                 445             


Asn Asn Arg Glu Leu Phe Phe Val Ala Ser Glu Ser Ser Tyr Asn Glu 
    450                 455                 460                 


Asn Asp Ile Asn Thr Pro Lys Glu Ile Asp Asp Thr Thr Asn Leu Asn 
465                 470                 475                 480 


Asn Asn Tyr Arg Asn Asn Leu Asp Glu Val Ile Leu Asp Tyr Asn Ser 
                485                 490                 495     


Glu Thr Ile Pro Gln Ile Ser Asn Gln Thr Leu Asn Thr Leu Val Gln 
            500                 505                 510         


Asp Asp Ser Tyr Val Pro Arg Tyr Asp Ser Asn Gly Thr Ser Glu Ile 
        515                 520                 525             


Glu Glu His Asn Val Val Asp Leu Asn Val Phe Phe Tyr Leu His Ala 
    530                 535                 540                 


Gln Lys Val Pro Glu Gly Glu Thr Asn Ile Ser Leu Thr Ser Ser Ile 
545                 550                 555                 560 


Asp Thr Ala Leu Ser Glu Glu Ser Gln Val Tyr Thr Phe Phe Ser Ser 
                565                 570                 575     


Glu Phe Ile Asn Thr Ile Asn Lys Pro Val His Ala Ala Leu Phe Ile 
            580                 585                 590         


Ser Trp Ile Asn Gln Val Ile Arg Asp Phe Thr Thr Glu Ala Thr Gln 
        595                 600                 605             


Lys Ser Thr Phe Asp Lys Ile Ala Asp Ile Ser Leu Val Val Pro Tyr 
    610                 615                 620                 


Val Gly Leu Ala Leu Asn Ile Gly Asn Glu Val Gln Lys Glu Asn Phe 
625                 630                 635                 640 


Lys Glu Ala Phe Glu Leu Leu Gly Ala Gly Ile Leu Leu Glu Phe Val 
                645                 650                 655     


Pro Glu Leu Leu Ile Pro Thr Ile Leu Val Phe Thr Ile Lys Ser Phe 
            660                 665                 670         


Ile Gly Ser Ser Glu Asn Lys Asn Lys Ile Ile Lys Ala Ile Asn Asn 
        675                 680                 685             


Ser Leu Met Glu Arg Glu Thr Lys Trp Lys Glu Ile Tyr Ser Trp Ile 
    690                 695                 700                 


Val Ser Asn Trp Leu Thr Arg Ile Asn Thr Gln Phe Asn Lys Arg Lys 
705                 710                 715                 720 


Glu Gln Met Tyr Gln Ala Leu Gln Asn Gln Val Asp Ala Ile Lys Thr 
                725                 730                 735     


Val Ile Glu Tyr Lys Tyr Asn Asn Tyr Thr Ser Asp Glu Arg Asn Arg 
            740                 745                 750         


Leu Glu Ser Glu Tyr Asn Ile Asn Asn Ile Arg Glu Glu Leu Asn Lys 
        755                 760                 765             


Lys Val Ser Leu Ala Met Glu Asn Ile Glu Arg Phe Ile Thr Glu Ser 
    770                 775                 780                 


Ser Ile Phe Tyr Leu Met Lys Leu Ile Asn Glu Ala Lys Val Ser Lys 
785                 790                 795                 800 


Leu Arg Glu Tyr Asp Glu Gly Val Lys Glu Tyr Leu Leu Asp Tyr Ile 
                805                 810                 815     


Ser Glu His Arg Ser Ile Leu Gly Asn Ser Val Gln Glu Leu Asn Asp 
            820                 825                 830         


Leu Val Thr Ser Thr Leu Asn Asn Ser Ile Pro Phe Glu Leu Ser Ser 
        835                 840                 845             


Tyr Thr Asn Asp Lys Ile Leu Ile Leu Tyr Phe Asn Lys Leu Tyr Lys 
    850                 855                 860                 


Lys Ile Lys Asp Asn Ser Ile Leu Asp Met Arg Tyr Glu Asn Asn Lys 
865                 870                 875                 880 


Phe Ile Asp Ile Ser Gly Tyr Gly Ser Asn Ile Ser Ile Asn Gly Asp 
                885                 890                 895     


Val Tyr Ile Tyr Ser Thr Asn Arg Asn Gln Phe Gly Ile Tyr Ser Ser 
            900                 905                 910         


Lys Pro Ser Glu Val Asn Ile Ala Gln Asn Asn Asp Ile Ile Tyr Asn 
        915                 920                 925             


Gly Arg Tyr Gln Asn Phe Ser Ile Ser Phe Trp Val Arg Ile Pro Lys 
    930                 935                 940                 


Tyr Phe Asn Lys Val Asn Leu Asn Asn Glu Tyr Thr Ile Ile Asp Cys 
945                 950                 955                 960 


Ile Arg Asn Asn Asn Ser Gly Trp Lys Ile Ser Leu Asn Tyr Asn Lys 
                965                 970                 975     


Ile Ile Trp Thr Leu Gln Asp Thr Ala Gly Asn Asn Gln Lys Leu Val 
            980                 985                 990         


Phe Asn Tyr Thr Gln Met Ile Ser  Ile Ser Asp Tyr Ile  Asn Lys Trp 
        995                 1000                 1005             


Ile Phe  Val Thr Ile Thr Asn  Asn Arg Leu Gly Asn  Ser Arg Ile 
    1010                 1015                 1020             


Tyr Ile  Asn Gly Asn Leu Ile  Asp Glu Lys Ser Ile  Ser Asn Leu 
    1025                 1030                 1035             


Gly Asp  Ile His Val Ser Asp  Asn Ile Leu Phe Lys  Ile Val Gly 
    1040                 1045                 1050             


Cys Asn  Asp Thr Arg Tyr Val  Gly Ile Arg Tyr Phe  Lys Val Phe 
    1055                 1060                 1065             


Asp Thr  Glu Leu Gly Lys Thr  Glu Ile Glu Thr Leu  Tyr Ser Asp 
    1070                 1075                 1080             


Glu Pro  Asp Pro Ser Ile Leu  Lys Asp Phe Trp Gly  Asn Tyr Leu 
    1085                 1090                 1095             


Leu Tyr  Asn Lys Arg Tyr Tyr  Leu Leu Asn Leu Leu  Arg Thr Asp 
    1100                 1105                 1110             


Lys Ser  Ile Thr Gln Asn Ser  Asn Phe Leu Asn Ile  Asn Gln Gln 
    1115                 1120                 1125             


Arg Gly  Val Tyr Gln Lys Pro  Asn Ile Phe Ser Asn  Thr Arg Leu 
    1130                 1135                 1140             


Tyr Thr  Gly Val Glu Val Ile  Ile Arg Lys Asn Gly  Ser Thr Asp 
    1145                 1150                 1155             


Ile Ser  Asn Thr Asp Asn Phe  Val Arg Lys Asn Asp  Leu Ala Tyr 
    1160                 1165                 1170             


Ile Asn  Val Val Asp Arg Asp  Val Glu Tyr Arg Leu  Tyr Ala Asp 
    1175                 1180                 1185             


Ile Ser  Ile Ala Lys Pro Glu  Lys Ile Ile Lys Leu  Ile Arg Thr 
    1190                 1195                 1200             


Ser Asn  Ser Asn Asn Ser Leu  Gly Gln Ile Ile Val  Met Asp Ser 
    1205                 1210                 1215             


Ile Gly  Asn Asn Cys Thr Met  Asn Phe Gln Asn Asn  Asn Gly Gly 
    1220                 1225                 1230             


Asn Ile  Gly Leu Leu Gly Phe  His Ser Asn Asn Leu  Val Ala Ser 
    1235                 1240                 1245             


Ser Trp  Tyr Tyr Asn Asn Ile  Arg Lys Asn Thr Ser  Ser Asn Gly 
    1250                 1255                 1260             


Cys Phe  Trp Ser Phe Ile Ser  Lys Glu His Gly Trp  Gln Glu Asn 
    1265                 1270                 1275             


<210>  23
<211>  1297
<212>  PRT
<213>  Clostridium botulinum


<220>
<221>  MISC_FEATURE
<222>  (7)..(7)
<223>  Xaa is any amino acid

<400>  23

Met Pro Val Asn Ile Lys Xaa Phe Asn Tyr Asn Asp Pro Ile Asn Asn 
1               5                   10                  15      


Asp Asp Ile Ile Met Met Glu Pro Phe Asn Asp Pro Gly Pro Gly Thr 
            20                  25                  30          


Tyr Tyr Lys Ala Phe Arg Ile Ile Asp Arg Ile Trp Ile Val Pro Glu 
        35                  40                  45              


Arg Phe Thr Tyr Gly Phe Gln Pro Asp Gln Phe Asn Ala Ser Thr Gly 
    50                  55                  60                  


Val Phe Ser Lys Asp Val Tyr Glu Tyr Tyr Asp Pro Thr Tyr Leu Lys 
65                  70                  75                  80  


Thr Asp Ala Glu Lys Asp Lys Phe Leu Lys Thr Met Ile Lys Leu Phe 
                85                  90                  95      


Asn Arg Ile Asn Ser Lys Pro Ser Gly Gln Arg Leu Leu Asp Met Ile 
            100                 105                 110         


Val Asp Ala Ile Pro Tyr Leu Gly Asn Ala Ser Thr Pro Pro Asp Lys 
        115                 120                 125             


Phe Ala Ala Asn Val Ala Asn Val Ser Ile Asn Lys Lys Ile Ile Gln 
    130                 135                 140                 


Pro Gly Ala Glu Asp Gln Ile Lys Gly Leu Met Thr Asn Leu Ile Ile 
145                 150                 155                 160 


Phe Gly Pro Gly Pro Val Leu Ser Asp Asn Phe Thr Asp Ser Met Ile 
                165                 170                 175     


Met Asn Gly His Ser Pro Ile Ser Glu Gly Phe Gly Ala Arg Met Met 
            180                 185                 190         


Ile Arg Phe Cys Pro Ser Cys Leu Asn Val Phe Asn Asn Val Gln Glu 
        195                 200                 205             


Asn Lys Asp Thr Ser Ile Phe Ser Arg Arg Ala Tyr Phe Ala Asp Pro 
    210                 215                 220                 


Ala Leu Thr Leu Met His Glu Leu Ile His Val Leu His Gly Leu Tyr 
225                 230                 235                 240 


Gly Ile Lys Ile Ser Asn Leu Pro Ile Thr Pro Asn Thr Lys Glu Phe 
                245                 250                 255     


Phe Met Gln His Ser Asp Pro Val Gln Ala Glu Glu Leu Tyr Thr Phe 
            260                 265                 270         


Gly Gly His Asp Pro Ser Val Ile Ser Pro Ser Thr Asp Met Asn Ile 
        275                 280                 285             


Tyr Asn Lys Ala Leu Gln Asn Phe Gln Asp Ile Ala Asn Arg Leu Asn 
    290                 295                 300                 


Ile Val Ser Ser Ala Gln Gly Ser Gly Ile Asp Ile Ser Leu Tyr Lys 
305                 310                 315                 320 


Gln Ile Tyr Lys Asn Lys Tyr Asp Phe Val Glu Asp Pro Asn Gly Lys 
                325                 330                 335     


Tyr Ser Val Asp Lys Asp Lys Phe Asp Lys Leu Tyr Lys Ala Leu Met 
            340                 345                 350         


Phe Gly Phe Thr Glu Thr Asn Leu Ala Gly Glu Tyr Gly Ile Lys Thr 
        355                 360                 365             


Arg Tyr Ser Tyr Phe Ser Glu Tyr Leu Pro Pro Ile Lys Thr Glu Lys 
    370                 375                 380                 


Leu Leu Asp Asn Thr Ile Tyr Thr Gln Asn Glu Gly Phe Asn Ile Ala 
385                 390                 395                 400 


Ser Lys Asn Leu Lys Thr Glu Phe Asn Gly Gln Asn Lys Ala Val Asn 
                405                 410                 415     


Lys Glu Ala Tyr Glu Glu Ile Ser Leu Glu His Leu Val Ile Tyr Arg 
            420                 425                 430         


Ile Ala Met Cys Lys Pro Val Met Tyr Lys Asn Thr Gly Lys Ser Glu 
        435                 440                 445             


Gln Cys Ile Ile Val Asn Asn Glu Asp Leu Phe Phe Ile Ala Asn Lys 
    450                 455                 460                 


Asp Ser Phe Ser Lys Asp Leu Ala Lys Ala Glu Thr Ile Ala Tyr Asn 
465                 470                 475                 480 


Thr Gln Asn Asn Thr Ile Glu Asn Asn Phe Ser Ile Asp Gln Leu Ile 
                485                 490                 495     


Leu Asp Asn Asp Leu Ser Ser Gly Ile Asp Leu Pro Asn Glu Asn Thr 
            500                 505                 510         


Glu Pro Phe Thr Asn Phe Asp Asp Ile Asp Ile Pro Val Tyr Ile Lys 
        515                 520                 525             


Gln Ser Ala Leu Lys Lys Ile Phe Val Asp Gly Asp Ser Leu Phe Glu 
    530                 535                 540                 


Tyr Leu His Ala Gln Thr Phe Pro Ser Asn Ile Glu Asn Leu Gln Leu 
545                 550                 555                 560 


Thr Asn Ser Leu Asn Asp Ala Leu Arg Asn Asn Asn Lys Val Tyr Thr 
                565                 570                 575     


Phe Phe Ser Thr Asn Leu Val Glu Lys Ala Asn Thr Val Val Gly Ala 
            580                 585                 590         


Ser Leu Phe Val Asn Trp Val Lys Gly Val Ile Asp Asp Phe Thr Ser 
        595                 600                 605             


Glu Ser Thr Gln Lys Ser Thr Ile Asp Lys Val Ser Asp Val Ser Ile 
    610                 615                 620                 


Ile Ile Pro Tyr Ile Gly Pro Ala Leu Asn Val Gly Asn Glu Thr Ala 
625                 630                 635                 640 


Lys Glu Asn Phe Lys Asn Ala Phe Glu Ile Gly Gly Ala Ala Ile Leu 
                645                 650                 655     


Met Glu Phe Ile Pro Glu Leu Ile Val Pro Ile Val Gly Phe Phe Thr 
            660                 665                 670         


Leu Glu Ser Tyr Val Gly Asn Lys Gly His Ile Ile Met Thr Ile Ser 
        675                 680                 685             


Asn Ala Leu Lys Lys Arg Asp Gln Lys Trp Thr Asp Met Tyr Gly Leu 
    690                 695                 700                 


Ile Val Ser Gln Trp Leu Ser Thr Val Asn Thr Gln Phe Tyr Thr Ile 
705                 710                 715                 720 


Lys Glu Arg Met Tyr Asn Ala Leu Asn Asn Gln Ser Gln Ala Ile Glu 
                725                 730                 735     


Lys Ile Ile Glu Asp Gln Tyr Asn Arg Tyr Ser Glu Glu Asp Lys Met 
            740                 745                 750         


Asn Ile Asn Ile Asp Phe Asn Asp Ile Asp Phe Lys Leu Asn Gln Ser 
        755                 760                 765             


Ile Asn Leu Ala Ile Asn Asn Ile Asp Asp Phe Ile Asn Gln Cys Ser 
    770                 775                 780                 


Ile Ser Tyr Leu Met Asn Arg Met Ile Pro Leu Ala Val Lys Lys Leu 
785                 790                 795                 800 


Lys Asp Phe Asp Asp Asn Leu Lys Arg Asp Leu Leu Glu Tyr Ile Asp 
                805                 810                 815     


Thr Asn Glu Leu Tyr Leu Leu Asp Glu Val Asn Ile Leu Lys Ser Lys 
            820                 825                 830         


Val Asn Arg His Leu Lys Asp Ser Ile Pro Phe Asp Leu Ser Leu Tyr 
        835                 840                 845             


Thr Lys Asp Thr Ile Leu Ile Gln Val Phe Asn Asn Tyr Ile Ser Asn 
    850                 855                 860                 


Ile Ser Ser Asn Ala Ile Leu Ser Leu Ser Tyr Arg Gly Gly Arg Leu 
865                 870                 875                 880 


Ile Asp Ser Ser Gly Tyr Gly Ala Thr Met Asn Val Gly Ser Asp Val 
                885                 890                 895     


Ile Phe Asn Asp Ile Gly Asn Gly Gln Phe Lys Leu Asn Asn Ser Glu 
            900                 905                 910         


Asn Ser Asn Ile Thr Ala His Gln Ser Lys Phe Val Val Tyr Asp Ser 
        915                 920                 925             


Met Phe Asp Asn Phe Ser Ile Asn Phe Trp Val Arg Thr Pro Lys Tyr 
    930                 935                 940                 


Asn Asn Asn Asp Ile Gln Thr Tyr Leu Gln Asn Glu Tyr Thr Ile Ile 
945                 950                 955                 960 


Ser Cys Ile Lys Asn Asp Ser Gly Trp Lys Val Ser Ile Lys Gly Asn 
                965                 970                 975     


Arg Ile Ile Trp Thr Leu Ile Asp Val Asn Ala Lys Ser Lys Ser Ile 
            980                 985                 990         


Phe Phe Glu Tyr Ser Ile Lys Asp  Asn Ile Ser Asp Tyr  Ile Asn Lys 
        995                 1000                 1005             


Trp Phe  Ser Ile Thr Ile Thr  Asn Asp Arg Leu Gly  Asn Ala Asn 
    1010                 1015                 1020             


Ile Tyr  Ile Asn Gly Ser Leu  Lys Lys Ser Glu Lys  Ile Leu Asn 
    1025                 1030                 1035             


Leu Asp  Arg Ile Asn Ser Ser  Asn Asp Ile Asp Phe  Lys Leu Ile 
    1040                 1045                 1050             


Asn Cys  Thr Asp Thr Thr Lys  Phe Val Trp Ile Lys  Asp Phe Asn 
    1055                 1060                 1065             


Ile Phe  Gly Arg Glu Leu Asn  Ala Thr Glu Val Ser  Ser Leu Tyr 
    1070                 1075                 1080             


Trp Ile  Gln Ser Ser Thr Asn  Thr Leu Lys Asp Phe  Trp Gly Asn 
    1085                 1090                 1095             


Pro Leu  Arg Tyr Asp Thr Gln  Tyr Tyr Leu Phe Asn  Gln Gly Met 
    1100                 1105                 1110             


Gln Asn  Ile Tyr Ile Lys Tyr  Phe Ser Lys Ala Ser  Met Gly Glu 
    1115                 1120                 1125             


Thr Ala  Pro Arg Thr Asn Phe  Asn Asn Ala Ala Ile  Asn Tyr Gln 
    1130                 1135                 1140             


Asn Leu  Tyr Leu Gly Leu Arg  Phe Ile Ile Lys Lys  Ala Ser Asn 
    1145                 1150                 1155             


Ser Arg  Asn Ile Asn Asn Asp  Asn Ile Val Arg Glu  Gly Asp Tyr 
    1160                 1165                 1170             


Ile Tyr  Leu Asn Ile Asp Asn  Ile Ser Asp Glu Ser  Tyr Arg Val 
    1175                 1180                 1185             


Tyr Val  Leu Val Asn Ser Lys  Glu Ile Gln Thr Gln  Leu Phe Leu 
    1190                 1195                 1200             


Ala Pro  Ile Asn Asp Asp Pro  Thr Phe Tyr Asp Val  Leu Gln Ile 
    1205                 1210                 1215             


Lys Lys  Tyr Tyr Glu Lys Thr  Thr Tyr Asn Cys Gln  Ile Leu Cys 
    1220                 1225                 1230             


Glu Lys  Asp Thr Lys Thr Phe  Gly Leu Phe Gly Ile  Gly Lys Phe 
    1235                 1240                 1245             


Val Lys  Asp Tyr Gly Tyr Val  Trp Asp Thr Tyr Asp  Asn Tyr Phe 
    1250                 1255                 1260             


Cys Ile  Ser Gln Trp Tyr Leu  Arg Arg Ile Ser Glu  Asn Ile Asn 
    1265                 1270                 1275             


Lys Leu  Arg Leu Gly Cys Asn  Trp Gln Phe Ile Pro  Val Asp Glu 
    1280                 1285                 1290             


Gly Trp  Thr Glu 
    1295         


<210>  24
<211>  1306
<212>  PRT
<213>  Clostridium botulinum

<400>  24

Met Lys Leu Glu Ile Asn Lys Phe Asn Tyr Asn Asp Pro Ile Asp Gly 
1               5                   10                  15      


Ile Asn Val Ile Thr Met Arg Pro Pro Arg His Ser Asp Lys Ile Asn 
            20                  25                  30          


Lys Gly Lys Gly Pro Phe Lys Ala Phe Gln Val Ile Lys Asn Ile Trp 
        35                  40                  45              


Ile Val Pro Glu Arg Tyr Asn Phe Thr Asn Asn Thr Asn Asp Leu Asn 
    50                  55                  60                  


Ile Pro Ser Glu Pro Ile Met Glu Ala Asp Ala Ile Tyr Asn Pro Asn 
65                  70                  75                  80  


Tyr Leu Asn Thr Pro Ser Glu Lys Asp Glu Phe Leu Gln Gly Val Ile 
                85                  90                  95      


Lys Val Leu Glu Arg Ile Lys Ser Lys Pro Glu Gly Glu Lys Leu Leu 
            100                 105                 110         


Glu Leu Ile Ser Ser Ser Ile Pro Leu Pro Leu Val Ser Asn Gly Ala 
        115                 120                 125             


Leu Thr Leu Ser Asp Asn Glu Thr Ile Ala Tyr Gln Glu Asn Asn Asn 
    130                 135                 140                 


Ile Val Ser Asn Leu Gln Ala Asn Leu Val Ile Tyr Gly Pro Gly Pro 
145                 150                 155                 160 


Asp Ile Ala Asn Asn Ala Thr Tyr Gly Leu Tyr Ser Thr Pro Ile Ser 
                165                 170                 175     


Asn Gly Glu Gly Thr Leu Ser Glu Val Ser Phe Ser Pro Phe Tyr Leu 
            180                 185                 190         


Lys Pro Phe Asp Glu Ser Tyr Gly Asn Tyr Arg Ser Leu Val Asn Ile 
        195                 200                 205             


Val Asn Lys Phe Val Lys Arg Glu Phe Ala Pro Asp Pro Ala Ser Thr 
    210                 215                 220                 


Leu Met His Glu Leu Val His Val Thr His Asn Leu Tyr Gly Ile Ser 
225                 230                 235                 240 


Asn Arg Asn Phe Tyr Tyr Asn Phe Asp Thr Gly Lys Ile Glu Thr Ser 
                245                 250                 255     


Arg Gln Gln Asn Ser Leu Ile Phe Glu Glu Leu Leu Thr Phe Gly Gly 
            260                 265                 270         


Ile Asp Ser Lys Ala Ile Ser Ser Leu Ile Ile Lys Lys Ile Ile Glu 
        275                 280                 285             


Thr Ala Lys Asn Asn Tyr Thr Thr Leu Ile Ser Glu Arg Leu Asn Thr 
    290                 295                 300                 


Val Thr Val Glu Asn Asp Leu Leu Lys Tyr Ile Lys Asn Lys Ile Pro 
305                 310                 315                 320 


Val Gln Gly Arg Leu Gly Asn Phe Lys Leu Asp Thr Ala Glu Phe Glu 
                325                 330                 335     


Lys Lys Leu Asn Thr Ile Leu Phe Val Leu Asn Glu Ser Asn Leu Ala 
            340                 345                 350         


Gln Arg Phe Ser Ile Leu Val Arg Lys His Tyr Leu Lys Glu Arg Pro 
        355                 360                 365             


Ile Asp Pro Ile Tyr Val Asn Ile Leu Asp Asp Asn Ser Tyr Ser Thr 
    370                 375                 380                 


Leu Glu Gly Phe Asn Ile Ser Ser Gln Gly Ser Asn Asp Phe Gln Gly 
385                 390                 395                 400 


Gln Leu Leu Glu Ser Ser Tyr Phe Glu Lys Ile Glu Ser Asn Ala Leu 
                405                 410                 415     


Arg Ala Phe Ile Lys Ile Cys Pro Arg Asn Gly Leu Leu Tyr Asn Ala 
            420                 425                 430         


Ile Tyr Arg Asn Ser Lys Asn Tyr Leu Asn Asn Ile Asp Leu Glu Asp 
        435                 440                 445             


Lys Lys Thr Thr Ser Lys Thr Asn Val Ser Tyr Pro Cys Ser Leu Leu 
    450                 455                 460                 


Asn Gly Cys Ile Glu Val Glu Asn Lys Asp Leu Phe Leu Ile Ser Asn 
465                 470                 475                 480 


Lys Asp Ser Leu Asn Asp Ile Asn Leu Ser Glu Glu Lys Ile Lys Pro 
                485                 490                 495     


Glu Thr Thr Val Phe Phe Lys Asp Lys Leu Pro Pro Gln Asp Ile Thr 
            500                 505                 510         


Leu Ser Asn Tyr Asp Phe Thr Glu Ala Asn Ser Ile Pro Ser Ile Ser 
        515                 520                 525             


Gln Gln Asn Ile Leu Glu Arg Asn Glu Glu Leu Tyr Glu Pro Ile Arg 
    530                 535                 540                 


Asn Ser Leu Phe Glu Ile Lys Thr Ile Tyr Val Asp Lys Leu Thr Thr 
545                 550                 555                 560 


Phe His Phe Leu Glu Ala Gln Asn Ile Asp Glu Ser Ile Asp Ser Ser 
                565                 570                 575     


Lys Ile Arg Val Glu Leu Thr Asp Ser Val Asp Glu Ala Leu Ser Asn 
            580                 585                 590         


Pro Asn Lys Val Tyr Ser Pro Phe Lys Asn Met Ser Asn Thr Ile Asn 
        595                 600                 605             


Ser Ile Glu Thr Gly Ile Thr Ser Thr Tyr Ile Phe Tyr Gln Trp Leu 
    610                 615                 620                 


Arg Ser Ile Val Lys Asp Phe Ser Asp Glu Thr Gly Lys Ile Asp Val 
625                 630                 635                 640 


Ile Asp Lys Ser Ser Asp Thr Leu Ala Ile Val Pro Tyr Ile Gly Pro 
                645                 650                 655     


Leu Leu Asn Ile Gly Asn Asp Ile Arg His Gly Asp Phe Val Gly Ala 
            660                 665                 670         


Ile Glu Leu Ala Gly Ile Thr Ala Leu Leu Glu Tyr Val Pro Glu Phe 
        675                 680                 685             


Thr Ile Pro Ile Leu Val Gly Leu Glu Val Ile Gly Gly Glu Leu Ala 
    690                 695                 700                 


Arg Glu Gln Val Glu Ala Ile Val Asn Asn Ala Leu Asp Lys Arg Asp 
705                 710                 715                 720 


Gln Lys Trp Ala Glu Val Tyr Asn Ile Thr Lys Ala Gln Trp Trp Gly 
                725                 730                 735     


Thr Ile His Leu Gln Ile Asn Thr Arg Leu Ala His Thr Tyr Lys Ala 
            740                 745                 750         


Leu Ser Arg Gln Ala Asn Ala Ile Lys Met Asn Met Glu Phe Gln Leu 
        755                 760                 765             


Ala Asn Tyr Lys Gly Asn Ile Asp Asp Lys Ala Lys Ile Lys Asn Ala 
    770                 775                 780                 


Ile Ser Glu Thr Glu Ile Leu Leu Asn Lys Ser Val Glu Gln Ala Met 
785                 790                 795                 800 


Lys Asn Thr Glu Lys Phe Met Ile Lys Leu Ser Asn Ser Tyr Leu Thr 
                805                 810                 815     


Lys Glu Met Ile Pro Lys Val Gln Asp Asn Leu Lys Asn Phe Asp Leu 
            820                 825                 830         


Glu Thr Lys Lys Thr Leu Asp Lys Phe Ile Lys Glu Lys Glu Asp Ile 
        835                 840                 845             


Leu Gly Thr Asn Leu Ser Ser Ser Leu Arg Arg Lys Val Ser Ile Arg 
    850                 855                 860                 


Leu Asn Lys Asn Ile Ala Phe Asp Ile Asn Asp Ile Pro Phe Ser Glu 
865                 870                 875                 880 


Phe Asp Asp Leu Ile Asn Gln Tyr Lys Asn Glu Ile Glu Asp Tyr Glu 
                885                 890                 895     


Val Leu Asn Leu Gly Ala Glu Asp Gly Lys Ile Lys Asp Leu Ser Gly 
            900                 905                 910         


Thr Thr Ser Asp Ile Asn Ile Gly Ser Asp Ile Glu Leu Ala Asp Gly 
        915                 920                 925             


Arg Glu Asn Lys Ala Ile Lys Ile Lys Gly Ser Glu Asn Ser Thr Ile 
    930                 935                 940                 


Lys Ile Ala Met Asn Lys Tyr Leu Arg Phe Ser Ala Thr Asp Asn Phe 
945                 950                 955                 960 


Ser Ile Ser Phe Trp Ile Lys His Pro Lys Pro Thr Asn Leu Leu Asn 
                965                 970                 975     


Asn Gly Ile Glu Tyr Thr Leu Val Glu Asn Phe Asn Gln Arg Gly Trp 
            980                 985                 990         


Lys Ile Ser Ile Gln Asp Ser Lys  Leu Ile Trp Tyr Leu  Arg Asp His 
        995                 1000                 1005             


Asn Asn  Ser Ile Lys Ile Val  Thr Pro Asp Tyr Ile  Ala Phe Asn 
    1010                 1015                 1020             


Gly Trp  Asn Leu Ile Thr Ile  Thr Asn Asn Arg Ser  Lys Gly Ser 
    1025                 1030                 1035             


Ile Val  Tyr Val Asn Gly Ser  Lys Ile Glu Glu Lys  Asp Ile Ser 
    1040                 1045                 1050             


Ser Ile  Trp Asn Thr Glu Val  Asp Asp Pro Ile Ile  Phe Arg Leu 
    1055                 1060                 1065             


Lys Asn  Asn Arg Asp Thr Gln  Ala Phe Thr Leu Leu  Asp Gln Phe 
    1070                 1075                 1080             


Ser Ile  Tyr Arg Lys Glu Leu  Asn Gln Asn Glu Val  Val Lys Leu 
    1085                 1090                 1095             


Tyr Asn  Tyr Tyr Phe Asn Ser  Asn Tyr Ile Arg Asp  Ile Trp Gly 
    1100                 1105                 1110             


Asn Pro  Leu Gln Tyr Asn Lys  Lys Tyr Tyr Leu Gln  Thr Gln Asp 
    1115                 1120                 1125             


Lys Pro  Gly Lys Gly Leu Ile  Arg Glu Tyr Trp Ser  Ser Phe Gly 
    1130                 1135                 1140             


Tyr Asp  Tyr Val Ile Leu Ser  Asp Ser Lys Thr Ile  Thr Phe Pro 
    1145                 1150                 1155             


Asn Asn  Ile Arg Tyr Gly Ala  Leu Tyr Asn Gly Ser  Lys Val Leu 
    1160                 1165                 1170             


Ile Lys  Asn Ser Lys Lys Leu  Asp Gly Leu Val Arg  Asn Lys Asp 
    1175                 1180                 1185             


Phe Ile  Gln Leu Glu Ile Asp  Gly Tyr Asn Met Gly  Ile Ser Ala 
    1190                 1195                 1200             


Asp Arg  Phe Asn Glu Asp Thr  Asn Tyr Ile Gly Thr  Thr Tyr Gly 
    1205                 1210                 1215             


Thr Thr  His Asp Leu Thr Thr  Asp Phe Glu Ile Ile  Gln Arg Gln 
    1220                 1225                 1230             


Glu Lys  Tyr Arg Asn Tyr Cys  Gln Leu Lys Thr Pro  Tyr Asn Ile 
    1235                 1240                 1245             


Phe His  Lys Ser Gly Leu Met  Ser Thr Glu Thr Ser  Lys Pro Thr 
    1250                 1255                 1260             


Phe His  Asp Tyr Arg Asp Trp  Val Tyr Ser Ser Ala  Trp Tyr Phe 
    1265                 1270                 1275             


Gln Asn  Tyr Glu Asn Leu Asn  Leu Arg Lys His Thr  Lys Thr Asn 
    1280                 1285                 1290             


Trp Tyr  Phe Ile Pro Lys Asp  Glu Gly Trp Asp Glu  Asp 
    1295                 1300                 1305     


<210>  25
<211>  1315
<212>  PRT
<213>  Clostridium tetani

<400>  25

Met Pro Ile Thr Ile Asn Asn Phe Arg Tyr Ser Asp Pro Val Asn Asn 
1               5                   10                  15      


Asp Thr Ile Ile Met Met Glu Pro Pro Tyr Cys Lys Gly Leu Asp Ile 
            20                  25                  30          


Tyr Tyr Lys Ala Phe Lys Ile Thr Asp Arg Ile Trp Ile Val Pro Glu 
        35                  40                  45              


Arg Tyr Glu Phe Gly Thr Lys Pro Glu Asp Phe Asn Pro Pro Ser Ser 
    50                  55                  60                  


Leu Ile Glu Gly Ala Ser Glu Tyr Tyr Asp Pro Asn Tyr Leu Arg Thr 
65                  70                  75                  80  


Asp Ser Asp Lys Asp Arg Phe Leu Gln Thr Met Val Lys Leu Phe Asn 
                85                  90                  95      


Arg Ile Lys Asn Asn Val Ala Gly Glu Ala Leu Leu Asp Lys Ile Ile 
            100                 105                 110         


Asn Ala Ile Pro Tyr Leu Gly Asn Ser Tyr Ser Leu Leu Asp Lys Phe 
        115                 120                 125             


Asp Thr Asn Ser Asn Ser Val Ser Phe Asn Leu Leu Glu Gln Asp Pro 
    130                 135                 140                 


Ser Gly Ala Thr Thr Lys Ser Ala Met Leu Thr Asn Leu Ile Ile Phe 
145                 150                 155                 160 


Gly Pro Gly Pro Val Leu Asn Lys Asn Glu Val Arg Gly Ile Val Leu 
                165                 170                 175     


Arg Val Asp Asn Lys Asn Tyr Phe Pro Cys Arg Asp Gly Phe Gly Ser 
            180                 185                 190         


Ile Met Gln Met Ala Phe Cys Pro Glu Tyr Val Pro Thr Phe Asp Asn 
        195                 200                 205             


Val Ile Glu Asn Ile Thr Ser Leu Thr Ile Gly Lys Ser Lys Tyr Phe 
    210                 215                 220                 


Gln Asp Pro Ala Leu Leu Leu Met His Glu Leu Ile His Val Leu His 
225                 230                 235                 240 


Gly Leu Tyr Gly Met Gln Val Ser Ser His Glu Ile Ile Pro Ser Lys 
                245                 250                 255     


Gln Glu Ile Tyr Met Gln His Thr Tyr Pro Ile Ser Ala Glu Glu Leu 
            260                 265                 270         


Phe Thr Phe Gly Gly Gln Asp Ala Asn Leu Ile Ser Ile Asp Ile Lys 
        275                 280                 285             


Asn Asp Leu Tyr Glu Lys Thr Leu Asn Asp Tyr Lys Ala Ile Ala Asn 
    290                 295                 300                 


Lys Leu Ser Gln Val Thr Ser Cys Asn Asp Pro Asn Ile Asp Ile Asp 
305                 310                 315                 320 


Ser Tyr Lys Gln Ile Tyr Gln Gln Lys Tyr Gln Phe Asp Lys Asp Ser 
                325                 330                 335     


Asn Gly Gln Tyr Ile Val Asn Glu Asp Lys Phe Gln Ile Leu Tyr Asn 
            340                 345                 350         


Ser Ile Met Tyr Gly Phe Thr Glu Ile Glu Leu Gly Lys Lys Phe Asn 
        355                 360                 365             


Ile Lys Thr Arg Leu Ser Tyr Phe Ser Met Asn His Asp Pro Val Lys 
    370                 375                 380                 


Ile Pro Asn Leu Leu Asp Asp Thr Ile Tyr Asn Asp Thr Glu Gly Phe 
385                 390                 395                 400 


Asn Ile Glu Ser Lys Asp Leu Lys Ser Glu Tyr Lys Gly Gln Asn Met 
                405                 410                 415     


Arg Val Asn Thr Asn Ala Phe Arg Asn Val Asp Gly Ser Gly Leu Val 
            420                 425                 430         


Ser Lys Leu Ile Gly Leu Cys Lys Lys Ile Ile Pro Pro Thr Asn Ile 
        435                 440                 445             


Arg Glu Asn Leu Tyr Asn Arg Thr Ala Ser Leu Thr Asp Leu Gly Gly 
    450                 455                 460                 


Glu Leu Cys Ile Lys Ile Lys Asn Glu Asp Leu Thr Phe Ile Ala Glu 
465                 470                 475                 480 


Lys Asn Ser Phe Ser Glu Glu Pro Phe Gln Asp Glu Ile Val Ser Tyr 
                485                 490                 495     


Asn Thr Lys Asn Lys Pro Leu Asn Phe Asn Tyr Ser Leu Asp Lys Ile 
            500                 505                 510         


Ile Val Asp Tyr Asn Leu Gln Ser Lys Ile Thr Leu Pro Asn Asp Arg 
        515                 520                 525             


Thr Thr Pro Val Thr Lys Gly Ile Pro Tyr Ala Pro Glu Tyr Lys Ser 
    530                 535                 540                 


Asn Ala Ala Ser Thr Ile Glu Ile His Asn Ile Asp Asp Asn Thr Ile 
545                 550                 555                 560 


Tyr Gln Tyr Leu Tyr Ala Gln Lys Ser Pro Thr Thr Leu Gln Arg Ile 
                565                 570                 575     


Thr Met Thr Asn Ser Val Asp Asp Ala Leu Ile Asn Ser Thr Lys Ile 
            580                 585                 590         


Tyr Ser Tyr Phe Pro Ser Val Ile Ser Lys Val Asn Gln Gly Ala Gln 
        595                 600                 605             


Gly Ile Leu Phe Leu Gln Trp Val Arg Asp Ile Ile Asp Asp Phe Thr 
    610                 615                 620                 


Asn Glu Ser Ser Gln Lys Thr Thr Ile Asp Lys Ile Ser Asp Val Ser 
625                 630                 635                 640 


Thr Ile Val Pro Tyr Ile Gly Pro Ala Leu Asn Ile Val Lys Gln Gly 
                645                 650                 655     


Tyr Glu Gly Asn Phe Ile Gly Ala Leu Glu Thr Thr Gly Val Val Leu 
            660                 665                 670         


Leu Leu Glu Tyr Ile Pro Glu Ile Thr Leu Pro Val Ile Ala Ala Leu 
        675                 680                 685             


Ser Ile Ala Glu Ser Ser Thr Gln Lys Glu Lys Ile Ile Lys Thr Ile 
    690                 695                 700                 


Asp Asn Phe Leu Glu Lys Arg Tyr Glu Lys Trp Ile Glu Val Tyr Lys 
705                 710                 715                 720 


Leu Val Lys Ala Lys Trp Leu Gly Thr Val Asn Thr Gln Phe Gln Lys 
                725                 730                 735     


Arg Ser Tyr Gln Met Tyr Arg Ser Leu Glu Tyr Gln Val Asp Ala Ile 
            740                 745                 750         


Lys Lys Ile Ile Asp Tyr Glu Tyr Lys Ile Tyr Ser Gly Pro Asp Lys 
        755                 760                 765             


Glu Gln Ile Ala Asp Glu Ile Asn Asn Leu Lys Asn Lys Leu Glu Glu 
    770                 775                 780                 


Lys Ala Asn Lys Ala Met Ile Asn Ile Asn Ile Phe Met Arg Glu Ser 
785                 790                 795                 800 


Ser Arg Ser Phe Leu Val Asn Gln Met Ile Asn Glu Ala Lys Lys Gln 
                805                 810                 815     


Leu Leu Glu Phe Asp Thr Gln Ser Lys Asn Ile Leu Met Gln Tyr Ile 
            820                 825                 830         


Lys Ala Asn Ser Lys Phe Ile Gly Ile Thr Glu Leu Lys Lys Leu Glu 
        835                 840                 845             


Ser Lys Ile Asn Lys Val Phe Ser Thr Pro Ile Pro Phe Ser Tyr Ser 
    850                 855                 860                 


Lys Asn Leu Asp Cys Trp Val Asp Asn Glu Glu Asp Ile Asp Val Ile 
865                 870                 875                 880 


Leu Lys Lys Ser Thr Ile Leu Asn Leu Asp Ile Asn Asn Asp Ile Ile 
                885                 890                 895     


Ser Asp Ile Ser Gly Phe Asn Ser Ser Val Ile Thr Tyr Pro Asp Ala 
            900                 905                 910         


Gln Leu Val Pro Gly Ile Asn Gly Lys Ala Ile His Leu Val Asn Asn 
        915                 920                 925             


Glu Ser Ser Glu Val Ile Val His Lys Ala Met Asp Ile Glu Tyr Asn 
    930                 935                 940                 


Asp Met Phe Asn Asn Phe Thr Val Ser Phe Trp Leu Arg Val Pro Lys 
945                 950                 955                 960 


Val Ser Ala Ser His Leu Glu Gln Tyr Gly Thr Asn Glu Tyr Ser Ile 
                965                 970                 975     


Ile Ser Ser Met Lys Lys His Ser Leu Ser Ile Gly Ser Gly Trp Ser 
            980                 985                 990         


Val Ser Leu Lys Gly Asn Asn Leu  Ile Trp Thr Leu Lys  Asp Ser Ala 
        995                 1000                 1005             


Gly Glu  Val Arg Gln Ile Thr  Phe Arg Asp Leu Pro  Asp Lys Phe 
    1010                 1015                 1020             


Asn Ala  Tyr Leu Ala Asn Lys  Trp Val Phe Ile Thr  Ile Thr Asn 
    1025                 1030                 1035             


Asp Arg  Leu Ser Ser Ala Asn  Leu Tyr Ile Asn Gly  Val Leu Met 
    1040                 1045                 1050             


Gly Ser  Ala Glu Ile Thr Gly  Leu Gly Ala Ile Arg  Glu Asp Asn 
    1055                 1060                 1065             


Asn Ile  Thr Leu Lys Leu Asp  Arg Cys Asn Asn Asn  Asn Gln Tyr 
    1070                 1075                 1080             


Val Ser  Ile Asp Lys Phe Arg  Ile Phe Cys Lys Ala  Leu Asn Pro 
    1085                 1090                 1095             


Lys Glu  Ile Glu Lys Leu Tyr  Thr Ser Tyr Leu Ser  Ile Thr Phe 
    1100                 1105                 1110             


Leu Arg  Asp Phe Trp Gly Asn  Pro Leu Arg Tyr Asp  Thr Glu Tyr 
    1115                 1120                 1125             


Tyr Leu  Ile Pro Val Ala Ser  Ser Ser Lys Asp Val  Gln Leu Lys 
    1130                 1135                 1140             


Asn Ile  Thr Asp Tyr Met Tyr  Leu Thr Asn Ala Pro  Ser Tyr Thr 
    1145                 1150                 1155             


Asn Gly  Lys Leu Asn Ile Tyr  Tyr Arg Arg Leu Tyr  Asn Gly Leu 
    1160                 1165                 1170             


Lys Phe  Ile Ile Lys Arg Tyr  Thr Pro Asn Asn Glu  Ile Asp Ser 
    1175                 1180                 1185             


Phe Val  Lys Ser Gly Asp Phe  Ile Lys Leu Tyr Val  Ser Tyr Asn 
    1190                 1195                 1200             


Asn Asn  Glu His Ile Val Gly  Tyr Pro Lys Asp Gly  Asn Ala Phe 
    1205                 1210                 1215             


Asn Asn  Leu Asp Arg Ile Leu  Arg Val Gly Tyr Asn  Ala Pro Gly 
    1220                 1225                 1230             


Ile Pro  Leu Tyr Lys Lys Met  Glu Ala Val Lys Leu  Arg Asp Leu 
    1235                 1240                 1245             


Lys Thr  Tyr Ser Val Gln Leu  Lys Leu Tyr Asp Asp  Lys Asn Ala 
    1250                 1255                 1260             


Ser Leu  Gly Leu Val Gly Thr  His Asn Gly Gln Ile  Gly Asn Asp 
    1265                 1270                 1275             


Pro Asn  Arg Asp Ile Leu Ile  Ala Ser Asn Trp Tyr  Phe Asn His 
    1280                 1285                 1290             


Leu Lys  Asp Lys Ile Leu Gly  Cys Asp Trp Tyr Phe  Val Pro Thr 
    1295                 1300                 1305             


Asp Glu  Gly Trp Thr Asn Asp  
    1310                 1315 


<210>  26
<211>  974
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Polypeptide sequence of labelled EGF TM polypeptide


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  HiLyte555 detectable label conjugated to His

<220>
<221>  MISC_FEATURE
<222>  (974)..(974)
<223>  HiLyte488 detectable label conjugated to Lys

<400>  26

His His His His His His Leu Ala Glu Thr Gly Gly Ser Gly Gly Ser 
1               5                   10                  15      


Gly Gly Ser Glu Phe Val Asn Lys Gln Phe Asn Tyr Lys Asp Pro Val 
            20                  25                  30          


Asn Gly Val Asp Ile Ala Tyr Ile Lys Ile Pro Asn Ala Gly Gln Met 
        35                  40                  45              


Gln Pro Val Lys Ala Phe Lys Ile His Asn Lys Ile Trp Val Ile Pro 
    50                  55                  60                  


Glu Arg Asp Thr Phe Thr Asn Pro Glu Glu Gly Asp Leu Asn Pro Pro 
65                  70                  75                  80  


Pro Glu Ala Lys Gln Val Pro Val Ser Tyr Tyr Asp Ser Thr Tyr Leu 
                85                  90                  95      


Ser Thr Asp Asn Glu Lys Asp Asn Tyr Leu Lys Gly Val Thr Lys Leu 
            100                 105                 110         


Phe Glu Arg Ile Tyr Ser Thr Asp Leu Gly Arg Met Leu Leu Thr Ser 
        115                 120                 125             


Ile Val Arg Gly Ile Pro Phe Trp Gly Gly Ser Thr Ile Asp Thr Glu 
    130                 135                 140                 


Leu Lys Val Ile Asp Thr Asn Cys Ile Asn Val Ile Gln Pro Asp Gly 
145                 150                 155                 160 


Ser Tyr Arg Ser Glu Glu Leu Asn Leu Val Ile Ile Gly Pro Ser Ala 
                165                 170                 175     


Asp Ile Ile Gln Phe Glu Cys Lys Ser Phe Gly His Glu Val Leu Asn 
            180                 185                 190         


Leu Thr Arg Asn Gly Tyr Gly Ser Thr Gln Tyr Ile Arg Phe Ser Pro 
        195                 200                 205             


Asp Phe Thr Phe Gly Phe Glu Glu Ser Leu Glu Val Asp Thr Asn Pro 
    210                 215                 220                 


Leu Leu Gly Ala Gly Lys Phe Ala Thr Asp Pro Ala Val Thr Leu Ala 
225                 230                 235                 240 


His Glu Leu Ile His Ala Gly His Arg Leu Tyr Gly Ile Ala Ile Asn 
                245                 250                 255     


Pro Asn Arg Val Phe Lys Val Asn Thr Asn Ala Tyr Tyr Glu Met Ser 
            260                 265                 270         


Gly Leu Glu Val Ser Phe Glu Glu Leu Arg Thr Phe Gly Gly His Asp 
        275                 280                 285             


Ala Lys Phe Ile Asp Ser Leu Gln Glu Asn Glu Phe Arg Leu Tyr Tyr 
    290                 295                 300                 


Tyr Asn Lys Phe Lys Asp Ile Ala Ser Thr Leu Asn Lys Ala Lys Ser 
305                 310                 315                 320 


Ile Val Gly Thr Thr Ala Ser Leu Gln Tyr Met Lys Asn Val Phe Lys 
                325                 330                 335     


Glu Lys Tyr Leu Leu Ser Glu Asp Thr Ser Gly Lys Phe Ser Val Asp 
            340                 345                 350         


Lys Leu Lys Phe Asp Lys Leu Tyr Lys Met Leu Thr Glu Ile Tyr Thr 
        355                 360                 365             


Glu Asp Asn Phe Val Lys Phe Phe Lys Val Leu Asn Arg Lys Thr Tyr 
    370                 375                 380                 


Leu Asn Phe Asp Lys Ala Val Phe Lys Ile Asn Ile Val Pro Lys Val 
385                 390                 395                 400 


Asn Tyr Thr Ile Tyr Asp Gly Phe Asn Leu Arg Asn Thr Asn Leu Ala 
                405                 410                 415     


Ala Asn Phe Asn Gly Gln Asn Thr Glu Ile Asn Asn Met Asn Phe Thr 
            420                 425                 430         


Lys Leu Lys Asn Phe Thr Gly Leu Phe Glu Phe Tyr Lys Leu Leu Cys 
        435                 440                 445             


Val Asp Gly Ile Ile Thr Ser Lys Thr Lys Ser Leu Ile Glu Gly Arg 
    450                 455                 460                 


Asn Lys Ala Leu Asn Leu Gln Cys Ile Lys Val Asn Asn Trp Asp Leu 
465                 470                 475                 480 


Phe Phe Ser Pro Ser Glu Asp Asn Phe Thr Asn Asp Leu Asn Lys Gly 
                485                 490                 495     


Glu Glu Ile Thr Ser Asp Thr Asn Ile Glu Ala Ala Glu Glu Asn Ile 
            500                 505                 510         


Ser Leu Asp Leu Ile Gln Gln Tyr Tyr Leu Thr Phe Asn Phe Asp Asn 
        515                 520                 525             


Glu Pro Glu Asn Ile Ser Ile Glu Asn Leu Ser Ser Asp Ile Ile Gly 
    530                 535                 540                 


Gln Leu Glu Leu Met Pro Asn Ile Glu Arg Phe Pro Asn Gly Lys Lys 
545                 550                 555                 560 


Tyr Glu Leu Asp Lys Tyr Thr Met Phe His Tyr Leu Arg Ala Gln Glu 
                565                 570                 575     


Phe Glu His Gly Lys Ser Arg Ile Ala Leu Thr Asn Ser Val Asn Glu 
            580                 585                 590         


Ala Leu Leu Asn Pro Ser Arg Val Tyr Thr Phe Phe Ser Ser Asp Tyr 
        595                 600                 605             


Val Lys Lys Val Asn Lys Ala Thr Glu Ala Ala Met Phe Leu Gly Trp 
    610                 615                 620                 


Val Glu Gln Leu Val Tyr Asp Phe Thr Asp Glu Thr Ser Glu Val Ser 
625                 630                 635                 640 


Thr Thr Asp Lys Ile Ala Asp Ile Thr Ile Ile Ile Pro Tyr Ile Gly 
                645                 650                 655     


Pro Ala Leu Asn Ile Gly Asn Met Leu Tyr Lys Asp Asp Phe Val Gly 
            660                 665                 670         


Ala Leu Ile Phe Ser Gly Ala Val Ile Leu Leu Glu Phe Ile Pro Glu 
        675                 680                 685             


Ile Ala Ile Pro Val Leu Gly Thr Phe Ala Leu Val Ser Tyr Ile Ala 
    690                 695                 700                 


Asn Lys Val Leu Thr Val Gln Thr Ile Asp Asn Ala Leu Ser Lys Arg 
705                 710                 715                 720 


Asn Glu Lys Trp Asp Glu Val Tyr Lys Tyr Ile Val Thr Asn Trp Leu 
                725                 730                 735     


Ala Lys Val Asn Thr Gln Ile Asp Leu Ile Arg Lys Lys Met Lys Glu 
            740                 745                 750         


Ala Leu Glu Asn Gln Ala Glu Ala Thr Lys Ala Ile Ile Asn Tyr Gln 
        755                 760                 765             


Tyr Asn Gln Tyr Thr Glu Glu Glu Lys Asn Asn Ile Asn Phe Asn Ile 
    770                 775                 780                 


Asp Asp Leu Ser Ser Lys Leu Asn Glu Ser Ile Asn Lys Ala Met Ile 
785                 790                 795                 800 


Asn Ile Asn Lys Phe Leu Asn Gln Cys Ser Val Ser Tyr Leu Met Asn 
                805                 810                 815     


Ser Met Ile Pro Tyr Gly Val Lys Arg Leu Glu Asp Phe Asp Ala Ser 
            820                 825                 830         


Leu Lys Asp Ala Leu Leu Lys Tyr Ile Tyr Asp Asn Arg Gly Thr Leu 
        835                 840                 845             


Ile Gly Gln Val Asp Arg Leu Lys Asp Lys Val Asn Asn Thr Leu Ser 
    850                 855                 860                 


Thr Asp Ile Pro Phe Gln Leu Ser Lys Tyr Val Asp Asn Gln Arg Leu 
865                 870                 875                 880 


Leu Ser Thr Leu Glu Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
                885                 890                 895     


Gly Gly Gly Ser Ala Leu Asp Asn Ser Asp Pro Lys Cys Pro Leu Ser 
            900                 905                 910         


His Glu Gly Tyr Cys Leu Asn Asp Gly Val Cys Met Tyr Ile Gly Thr 
        915                 920                 925             


Leu Asp Arg Tyr Ala Cys Asn Cys Val Val Gly Tyr Val Gly Glu Arg 
    930                 935                 940                 


Cys Gln Tyr Arg Asp Leu Lys Leu Ala Glu Leu Arg Gly Leu Glu Ala 
945                 950                 955                 960 


Gly Gly Ser Gly Gly Gly Ser Gly Leu Pro Glu Ser Gly Lys 
                965                 970                 


<210>  27
<211>  482
<212>  PRT
<213>  Clitoria ternatea

<400>  27

Met Lys Asn Pro Leu Ala Ile Leu Phe Leu Ile Ala Thr Val Val Ala 
1               5                   10                  15      


Val Val Ser Gly Ile Arg Asp Asp Phe Leu Arg Leu Pro Ser Gln Ala 
            20                  25                  30          


Ser Lys Phe Phe Gln Ala Asp Asp Asn Val Glu Gly Thr Arg Trp Ala 
        35                  40                  45              


Val Leu Val Ala Gly Ser Lys Gly Tyr Val Asn Tyr Arg His Gln Ala 
    50                  55                  60                  


Asp Val Cys His Ala Tyr Gln Ile Leu Lys Lys Gly Gly Leu Lys Asp 
65                  70                  75                  80  


Glu Asn Ile Ile Val Phe Met Tyr Asp Asp Ile Ala Tyr Asn Glu Ser 
                85                  90                  95      


Asn Pro His Pro Gly Val Ile Ile Asn His Pro Tyr Gly Ser Asp Val 
            100                 105                 110         


Tyr Lys Gly Val Pro Lys Asp Tyr Val Gly Glu Asp Ile Asn Pro Pro 
        115                 120                 125             


Asn Phe Tyr Ala Val Leu Leu Ala Asn Lys Ser Ala Leu Thr Gly Thr 
    130                 135                 140                 


Gly Ser Gly Lys Val Leu Asp Ser Gly Pro Asn Asp His Val Phe Ile 
145                 150                 155                 160 


Tyr Tyr Thr Asp His Gly Gly Ala Gly Val Leu Gly Met Pro Ser Lys 
                165                 170                 175     


Pro Tyr Ile Ala Ala Ser Asp Leu Asn Asp Val Leu Lys Lys Lys His 
            180                 185                 190         


Ala Ser Gly Thr Tyr Lys Ser Ile Val Phe Tyr Val Glu Ser Cys Glu 
        195                 200                 205             


Ser Gly Ser Met Phe Asp Gly Leu Leu Pro Glu Asp His Asn Ile Tyr 
    210                 215                 220                 


Val Met Gly Ala Ser Asp Thr Gly Glu Ser Ser Trp Val Thr Tyr Cys 
225                 230                 235                 240 


Pro Leu Gln His Pro Ser Pro Pro Pro Glu Tyr Asp Val Cys Val Gly 
                245                 250                 255     


Asp Leu Phe Ser Val Ala Trp Leu Glu Asp Cys Asp Val His Asn Leu 
            260                 265                 270         


Gln Thr Glu Thr Phe Gln Gln Gln Tyr Glu Val Val Lys Asn Lys Thr 
        275                 280                 285             


Ile Val Ala Leu Ile Glu Asp Gly Thr His Val Val Gln Tyr Gly Asp 
    290                 295                 300                 


Val Gly Leu Ser Lys Gln Thr Leu Phe Val Tyr Met Gly Thr Asp Pro 
305                 310                 315                 320 


Ala Asn Asp Asn Asn Thr Phe Thr Asp Lys Asn Ser Leu Gly Thr Pro 
                325                 330                 335     


Arg Lys Ala Val Ser Gln Arg Asp Ala Asp Leu Ile His Tyr Trp Glu 
            340                 345                 350         


Lys Tyr Arg Arg Ala Pro Glu Gly Ser Ser Arg Lys Ala Glu Ala Lys 
        355                 360                 365             


Lys Gln Leu Arg Glu Val Met Ala His Arg Met His Ile Asp Asn Ser 
    370                 375                 380                 


Val Lys His Ile Gly Lys Leu Leu Phe Gly Ile Glu Lys Gly His Lys 
385                 390                 395                 400 


Met Leu Asn Asn Val Arg Pro Ala Gly Leu Pro Val Val Asp Asp Trp 
                405                 410                 415     


Asp Cys Phe Lys Thr Leu Ile Arg Thr Phe Glu Thr His Cys Gly Ser 
            420                 425                 430         


Leu Ser Glu Tyr Gly Met Lys His Met Arg Ser Phe Ala Asn Leu Cys 
        435                 440                 445             


Asn Ala Gly Ile Arg Lys Glu Gln Met Ala Glu Ala Ser Ala Gln Ala 
    450                 455                 460                 


Cys Val Ser Ile Pro Asp Asn Pro Trp Ser Ser Leu His Ala Gly Phe 
465                 470                 475                 480 


Ser Val 
        


<210>  28
<211>  462
<212>  PRT
<213>  Clitoria ternatea

<400>  28

Ile Arg Asp Asp Phe Leu Arg Leu Pro Ser Gln Ala Ser Lys Phe Phe 
1               5                   10                  15      


Gln Ala Asp Asp Asn Val Glu Gly Thr Arg Trp Ala Val Leu Val Ala 
            20                  25                  30          


Gly Ser Lys Gly Tyr Val Asn Tyr Arg His Gln Ala Asp Val Cys His 
        35                  40                  45              


Ala Tyr Gln Ile Leu Lys Lys Gly Gly Leu Lys Asp Glu Asn Ile Ile 
    50                  55                  60                  


Val Phe Met Tyr Asp Asp Ile Ala Tyr Asn Glu Ser Asn Pro His Pro 
65                  70                  75                  80  


Gly Val Ile Ile Asn His Pro Tyr Gly Ser Asp Val Tyr Lys Gly Val 
                85                  90                  95      


Pro Lys Asp Tyr Val Gly Glu Asp Ile Asn Pro Pro Asn Phe Tyr Ala 
            100                 105                 110         


Val Leu Leu Ala Asn Lys Ser Ala Leu Thr Gly Thr Gly Ser Gly Lys 
        115                 120                 125             


Val Leu Asp Ser Gly Pro Asn Asp His Val Phe Ile Tyr Tyr Thr Asp 
    130                 135                 140                 


His Gly Gly Ala Gly Val Leu Gly Met Pro Ser Lys Pro Tyr Ile Ala 
145                 150                 155                 160 


Ala Ser Asp Leu Asn Asp Val Leu Lys Lys Lys His Ala Ser Gly Thr 
                165                 170                 175     


Tyr Lys Ser Ile Val Phe Tyr Val Glu Ser Cys Glu Ser Gly Ser Met 
            180                 185                 190         


Phe Asp Gly Leu Leu Pro Glu Asp His Asn Ile Tyr Val Met Gly Ala 
        195                 200                 205             


Ser Asp Thr Gly Glu Ser Ser Trp Val Thr Tyr Cys Pro Leu Gln His 
    210                 215                 220                 


Pro Ser Pro Pro Pro Glu Tyr Asp Val Cys Val Gly Asp Leu Phe Ser 
225                 230                 235                 240 


Val Ala Trp Leu Glu Asp Cys Asp Val His Asn Leu Gln Thr Glu Thr 
                245                 250                 255     


Phe Gln Gln Gln Tyr Glu Val Val Lys Asn Lys Thr Ile Val Ala Leu 
            260                 265                 270         


Ile Glu Asp Gly Thr His Val Val Gln Tyr Gly Asp Val Gly Leu Ser 
        275                 280                 285             


Lys Gln Thr Leu Phe Val Tyr Met Gly Thr Asp Pro Ala Asn Asp Asn 
    290                 295                 300                 


Asn Thr Phe Thr Asp Lys Asn Ser Leu Gly Thr Pro Arg Lys Ala Val 
305                 310                 315                 320 


Ser Gln Arg Asp Ala Asp Leu Ile His Tyr Trp Glu Lys Tyr Arg Arg 
                325                 330                 335     


Ala Pro Glu Gly Ser Ser Arg Lys Ala Glu Ala Lys Lys Gln Leu Arg 
            340                 345                 350         


Glu Val Met Ala His Arg Met His Ile Asp Asn Ser Val Lys His Ile 
        355                 360                 365             


Gly Lys Leu Leu Phe Gly Ile Glu Lys Gly His Lys Met Leu Asn Asn 
    370                 375                 380                 


Val Arg Pro Ala Gly Leu Pro Val Val Asp Asp Trp Asp Cys Phe Lys 
385                 390                 395                 400 


Thr Leu Ile Arg Thr Phe Glu Thr His Cys Gly Ser Leu Ser Glu Tyr 
                405                 410                 415     


Gly Met Lys His Met Arg Ser Phe Ala Asn Leu Cys Asn Ala Gly Ile 
            420                 425                 430         


Arg Lys Glu Gln Met Ala Glu Ala Ser Ala Gln Ala Cys Val Ser Ile 
        435                 440                 445             


Pro Asp Asn Pro Trp Ser Ser Leu His Ala Gly Phe Ser Val 
    450                 455                 460         


<210>  29
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Peptide with conjugated detectable label and sortase donor site


<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  HiLyte488 detectable label conjugated to Lys

<400>  29

Gly Gly Gly Gly Lys 
1               5   


<210>  30
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Peptide with conjugated detectable label and sortase acceptor 
       site


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  HiLyte555 detectable label conjugated to His

<400>  30

His His His His His His Leu Ala Glu Thr Gly Gly Gly 
1               5                   10              


<210>  31
<211>  206
<212>  PRT
<213>  Staphylococcus aureus

<400>  31

Met Lys Lys Trp Thr Asn Arg Leu Met Thr Ile Ala Gly Val Val Leu 
1               5                   10                  15      


Ile Leu Val Ala Ala Tyr Leu Phe Ala Lys Pro His Ile Asp Asn Tyr 
            20                  25                  30          


Leu His Asp Lys Asp Lys Asp Glu Lys Ile Glu Gln Tyr Asp Lys Asn 
        35                  40                  45              


Val Lys Glu Gln Ala Ser Lys Asp Lys Lys Gln Gln Ala Lys Pro Gln 
    50                  55                  60                  


Ile Pro Lys Asp Lys Ser Lys Val Ala Gly Tyr Ile Glu Ile Pro Asp 
65                  70                  75                  80  


Ala Asp Ile Lys Glu Pro Val Tyr Pro Gly Pro Ala Thr Pro Glu Gln 
                85                  90                  95      


Leu Asn Arg Gly Val Ser Phe Ala Glu Glu Asn Glu Ser Leu Asp Asp 
            100                 105                 110         


Gln Asn Ile Ser Ile Ala Gly His Thr Phe Ile Asp Arg Pro Asn Tyr 
        115                 120                 125             


Gln Phe Thr Asn Leu Lys Ala Ala Lys Lys Gly Ser Met Val Tyr Phe 
    130                 135                 140                 


Lys Val Gly Asn Glu Thr Arg Lys Tyr Lys Met Thr Ser Ile Arg Asp 
145                 150                 155                 160 


Val Lys Pro Thr Asp Val Gly Val Leu Asp Glu Gln Lys Gly Lys Asp 
                165                 170                 175     


Lys Gln Leu Thr Leu Ile Thr Cys Asp Asp Tyr Asn Glu Lys Thr Gly 
            180                 185                 190         


Val Trp Glu Lys Arg Lys Ile Phe Val Ala Thr Glu Val Lys 
        195                 200                 205     


<210>  32
<211>  244
<212>  PRT
<213>  Staphylococcus aureus

<400>  32

Met Arg Met Lys Arg Phe Leu Thr Ile Val Gln Ile Leu Leu Val Val 
1               5                   10                  15      


Ile Ile Ile Ile Phe Gly Tyr Lys Ile Val Gln Thr Tyr Ile Glu Asp 
            20                  25                  30          


Lys Gln Glu Arg Ala Asn Tyr Glu Lys Leu Gln Gln Lys Phe Gln Met 
        35                  40                  45              


Leu Met Ser Lys His Gln Glu His Val Arg Pro Gln Phe Glu Ser Leu 
    50                  55                  60                  


Glu Lys Ile Asn Lys Asp Ile Val Gly Trp Ile Lys Leu Ser Gly Thr 
65                  70                  75                  80  


Ser Leu Asn Tyr Pro Val Leu Gln Gly Lys Thr Asn His Asp Tyr Leu 
                85                  90                  95      


Asn Leu Asp Phe Glu Arg Glu His Arg Arg Lys Gly Ser Ile Phe Met 
            100                 105                 110         


Asp Phe Arg Asn Glu Leu Lys Asn Leu Asn His Asn Thr Ile Leu Tyr 
        115                 120                 125             


Gly His His Val Gly Asp Asn Thr Met Phe Asp Val Leu Glu Asp Tyr 
    130                 135                 140                 


Leu Lys Gln Ser Phe Tyr Glu Lys His Lys Ile Ile Glu Phe Asp Asn 
145                 150                 155                 160 


Lys Tyr Gly Lys Tyr Gln Leu Gln Val Phe Ser Ala Tyr Lys Thr Thr 
                165                 170                 175     


Thr Lys Asp Asn Tyr Ile Arg Thr Asp Phe Glu Asn Asp Gln Asp Tyr 
            180                 185                 190         


Gln Gln Phe Leu Asp Glu Thr Lys Arg Lys Ser Val Ile Asn Ser Asp 
        195                 200                 205             


Val Asn Val Thr Val Lys Asp Arg Ile Met Thr Leu Ser Thr Cys Glu 
    210                 215                 220                 


Asp Ala Tyr Ser Glu Thr Thr Lys Arg Ile Val Val Val Ala Lys Ile 
225                 230                 235                 240 


Ile Lys Val Ser 
                


<210>  33
<211>  260
<212>  PRT
<213>  Streptococcus pneumoniae

<400>  33

Met Glu Lys Leu Tyr Ile His Leu Lys Asn Leu Arg Lys Val Ala Val 
1               5                   10                  15      


Val Met Leu Leu Val Phe Thr Thr Phe Tyr Leu Leu Leu Met Phe Leu 
            20                  25                  30          


Asn Gln Ser Asp Asn Gln Glu Ile Ala Lys Asn Ile Glu Lys Phe Asn 
        35                  40                  45              


Asp Ser Val Ile Val Ala Lys Thr Asp Asn Thr Lys Ala Asp Ile Lys 
    50                  55                  60                  


Glu Ile Glu Lys Asn Ile Glu Lys Val Arg Lys Ile Glu Gly Gly Asn 
65                  70                  75                  80  


Val Glu Arg Val Asn Gln Leu Thr Ser Glu Asn Glu Lys Val Lys Glu 
                85                  90                  95      


Asn Ile Asp Leu Asn Ile Glu Glu Glu Ile Ile Glu Asn Ser Tyr Lys 
            100                 105                 110         


Ser Leu Glu Thr Thr Asp Asn Phe Glu Lys Leu Gly Ile Ile Glu Ile 
        115                 120                 125             


Pro Lys Ile Asp Leu Asn Leu Ser Ile Phe Lys Gly Lys Pro Phe Val 
    130                 135                 140                 


Asn Thr Lys Asn Arg Gln Asp Thr Met Leu Tyr Gly Ala Val Thr Asn 
145                 150                 155                 160 


Lys Lys Asn Gln Lys Met Gly Arg Glu Asn Tyr Val Leu Ala Ser His 
                165                 170                 175     


Ile Ile Ser Asn Ser Asn Leu Leu Phe Thr Ser Ile Asn Gln Leu Glu 
            180                 185                 190         


Lys Gly Asp Val Ile Thr Leu Lys Asp Ser Glu Tyr Ser Tyr Gln Tyr 
        195                 200                 205             


Thr Val Tyr Asn Asn Phe Ile Val Ser Lys Asp Glu Thr Trp Ile Leu 
    210                 215                 220                 


Asn Asp Ile Lys Asp Tyr Ser Ile Leu Thr Leu Tyr Thr Cys Tyr Asp 
225                 230                 235                 240 


Asp Ser Thr Lys Leu Pro Glu Asn Arg Val Val Ile Arg Ala Val Leu 
                245                 250                 255     


Thr Asp Ile Asn 
            260 


<210>  34
<211>  298
<212>  PRT
<213>  Streptococcus pneumoniae


<220>
<221>  MISC_FEATURE
<222>  (22)..(22)
<223>  Xaa is Met or Ile

<400>  34

Met Ala Lys Thr Lys Lys Gln Lys Arg Asn Asn Leu Leu Leu Gly Val 
1               5                   10                  15      


Val Phe Phe Ile Gly Xaa Ala Val Met Ala Tyr Pro Leu Val Ser Arg 
            20                  25                  30          


Leu Tyr Tyr Arg Val Glu Ser Asn Gln Gln Ile Ala Asp Phe Asp Lys 
        35                  40                  45              


Glu Lys Ala Thr Leu Asp Glu Ala Asp Ile Asp Glu Arg Met Lys Leu 
    50                  55                  60                  


Ala Gln Ala Phe Asn Asp Ser Leu Asn Asn Val Val Ser Gly Asp Pro 
65                  70                  75                  80  


Trp Ser Glu Glu Met Lys Lys Lys Gly Arg Ala Glu Tyr Ala Arg Met 
                85                  90                  95      


Leu Glu Ile His Glu Arg Met Gly His Val Glu Ile Pro Ala Ile Asp 
            100                 105                 110         


Val Asp Leu Pro Val Tyr Ala Gly Thr Ala Glu Glu Val Leu Gln Gln 
        115                 120                 125             


Gly Ala Gly His Leu Glu Gly Thr Ser Leu Pro Ile Gly Gly Asn Ser 
    130                 135                 140                 


Thr His Ala Val Ile Thr Ala His Thr Gly Leu Pro Thr Ala Lys Met 
145                 150                 155                 160 


Phe Thr Asp Leu Thr Lys Leu Lys Val Gly Asp Lys Phe Tyr Val His 
                165                 170                 175     


Asn Ile Lys Glu Val Met Ala Tyr Gln Val Asp Gln Val Lys Val Ile 
            180                 185                 190         


Glu Pro Thr Asn Phe Asp Asp Leu Leu Ile Val Pro Gly His Asp Tyr 
        195                 200                 205             


Val Thr Leu Leu Thr Cys Thr Pro Tyr Met Ile Asn Thr His Arg Leu 
    210                 215                 220                 


Leu Val Arg Gly His Arg Ile Pro Tyr Val Ala Glu Val Glu Glu Glu 
225                 230                 235                 240 


Phe Ile Ala Ala Asn Lys Leu Ser His Leu Tyr Arg Tyr Leu Phe Tyr 
                245                 250                 255     


Val Ala Val Gly Leu Ile Val Ile Leu Leu Trp Ile Ile Arg Arg Leu 
            260                 265                 270         


Arg Lys Lys Lys Arg Gln Ser Glu Arg Ala Leu Lys Ala Leu Lys Glu 
        275                 280                 285             


Ala Thr Lys Glu Val Lys Val Glu Asp Glu 
    290                 295             


<210>  35
<211>  297
<212>  PRT
<213>  Streptococcus pneumoniae

<400>  35

Met Asp Asn Ser Arg Arg Ser Arg Lys Lys Gly Thr Lys Lys Lys Lys 
1               5                   10                  15      


His Pro Leu Ile Leu Leu Leu Ile Phe Leu Val Gly Phe Ala Val Ala 
            20                  25                  30          


Ile Tyr Pro Leu Val Ser Arg Tyr Tyr Tyr Arg Ile Glu Ser Asn Glu 
        35                  40                  45              


Val Ile Lys Glu Phe Asp Glu Thr Val Ser Gln Met Asp Lys Ala Glu 
    50                  55                  60                  


Leu Glu Glu Arg Trp Arg Leu Ala Gln Ala Phe Asn Ala Thr Leu Lys 
65                  70                  75                  80  


Pro Ser Glu Ile Leu Asp Pro Phe Thr Glu Gln Glu Lys Lys Lys Gly 
                85                  90                  95      


Val Ser Glu Tyr Ala Asn Met Leu Lys Val His Glu Arg Ile Gly Tyr 
            100                 105                 110         


Val Glu Ile Pro Ala Ile Asp Gln Glu Ile Pro Met Tyr Val Gly Thr 
        115                 120                 125             


Ser Glu Asp Ile Leu Gln Lys Gly Ala Gly Leu Leu Glu Gly Ala Ser 
    130                 135                 140                 


Leu Pro Val Gly Gly Lys Asn Thr His Thr Val Ile Thr Ala His Arg 
145                 150                 155                 160 


Gly Leu Pro Thr Ala Glu Leu Phe Ser Gln Leu Asp Lys Met Lys Lys 
                165                 170                 175     


Gly Asp Ile Phe Tyr Leu His Val Leu Asp Gln Val Leu Ala Tyr Gln 
            180                 185                 190         


Val Asp Gln Ile Val Thr Val Glu Pro Asn Asp Phe Glu Pro Val Leu 
        195                 200                 205             


Ile Gln His Gly Glu Asp Tyr Ala Thr Leu Leu Thr Cys Thr Pro Tyr 
    210                 215                 220                 


Met Ile Asn Ser His Arg Leu Leu Val Arg Gly Lys Arg Ile Pro Tyr 
225                 230                 235                 240 


Thr Ala Pro Ile Ala Glu Arg Asn Arg Ala Val Arg Glu Arg Gly Gln 
                245                 250                 255     


Phe Trp Leu Trp Leu Leu Leu Gly Ala Met Ala Val Ile Leu Leu Leu 
            260                 265                 270         


Leu Tyr Arg Val Tyr Arg Asn Arg Arg Ile Val Lys Gly Leu Glu Lys 
        275                 280                 285             


Gln Leu Glu Gly Arg His Val Lys Asp 
    290                 295         


<210>  36
<211>  283
<212>  PRT
<213>  Streptococcus pneumoniae

<400>  36

Met Ser Arg Thr Lys Leu Arg Ala Leu Leu Gly Tyr Leu Leu Met Leu 
1               5                   10                  15      


Val Ala Cys Leu Ile Pro Ile Tyr Cys Phe Gly Gln Met Val Leu Gln 
            20                  25                  30          


Ser Leu Gly Gln Val Lys Gly His Ala Thr Phe Val Lys Ser Met Thr 
        35                  40                  45              


Thr Glu Met Tyr Gln Glu Gln Gln Asn His Ser Leu Ala Tyr Asn Gln 
    50                  55                  60                  


Arg Leu Ala Ser Gln Asn Arg Ile Val Asp Pro Phe Leu Ala Glu Gly 
65                  70                  75                  80  


Tyr Glu Val Asn Tyr Gln Val Ser Asp Asp Pro Asp Ala Val Tyr Gly 
                85                  90                  95      


Tyr Leu Ser Ile Pro Ser Leu Glu Ile Met Glu Pro Val Tyr Leu Gly 
            100                 105                 110         


Ala Asp Tyr His His Leu Gly Met Gly Leu Ala His Val Asp Gly Thr 
        115                 120                 125             


Pro Leu Pro Met Asp Gly Thr Gly Ile Arg Ser Val Ile Ala Gly His 
    130                 135                 140                 


Arg Ala Glu Pro Ser His Val Phe Phe Arg His Leu Asp Gln Leu Lys 
145                 150                 155                 160 


Val Gly Asp Ala Leu Tyr Tyr Asp Asn Gly Gln Glu Ile Val Glu Tyr 
                165                 170                 175     


Gln Met Met Asp Thr Glu Ile Ile Leu Pro Ser Glu Trp Glu Lys Leu 
            180                 185                 190         


Glu Ser Val Ser Ser Lys Asn Ile Met Thr Leu Ile Thr Cys Asp Pro 
        195                 200                 205             


Ile Pro Thr Phe Asn Lys Arg Leu Leu Val Asn Phe Glu Arg Val Ala 
    210                 215                 220                 


Val Tyr Gln Lys Ser Asp Pro Gln Thr Ala Ala Val Ala Arg Val Ala 
225                 230                 235                 240 


Phe Thr Lys Glu Gly Gln Ser Val Ser Arg Val Ala Thr Ser Gln Trp 
                245                 250                 255     


Leu Tyr Arg Gly Leu Val Val Leu Ala Phe Leu Gly Ile Leu Phe Val 
            260                 265                 270         


Leu Trp Lys Leu Ala Arg Leu Leu Arg Gly Lys 
        275                 280             


<210>  37
<211>  249
<212>  PRT
<213>  Streptococcus pyogenes

<400>  37

Met Val Lys Lys Gln Lys Arg Arg Lys Ile Lys Ser Met Ser Trp Ala 
1               5                   10                  15      


Arg Lys Leu Leu Ile Ala Val Leu Leu Ile Leu Gly Leu Ala Leu Leu 
            20                  25                  30          


Phe Asn Lys Pro Ile Arg Asn Thr Leu Ile Ala Arg Asn Ser Asn Lys 
        35                  40                  45              


Tyr Gln Val Thr Lys Val Ser Lys Lys Gln Ile Lys Lys Asn Lys Glu 
    50                  55                  60                  


Ala Lys Ser Thr Phe Asp Phe Gln Ala Val Glu Pro Val Ser Thr Glu 
65                  70                  75                  80  


Ser Val Leu Gln Ala Gln Met Ala Ala Gln Gln Leu Pro Val Ile Gly 
                85                  90                  95      


Gly Ile Ala Ile Pro Glu Leu Gly Ile Asn Leu Pro Ile Phe Lys Gly 
            100                 105                 110         


Leu Gly Asn Thr Glu Leu Ile Tyr Gly Ala Gly Thr Met Lys Glu Glu 
        115                 120                 125             


Gln Val Met Gly Gly Glu Asn Asn Tyr Ser Leu Ala Ser His His Ile 
    130                 135                 140                 


Phe Gly Ile Thr Gly Ser Ser Gln Met Leu Phe Ser Pro Leu Glu Arg 
145                 150                 155                 160 


Ala Gln Asn Gly Met Ser Ile Tyr Leu Thr Asp Lys Glu Lys Ile Tyr 
                165                 170                 175     


Glu Tyr Ile Ile Lys Asp Val Phe Thr Val Ala Pro Glu Arg Val Asp 
            180                 185                 190         


Val Ile Asp Asp Thr Ala Gly Leu Lys Glu Val Thr Leu Val Thr Cys 
        195                 200                 205             


Thr Asp Ile Glu Ala Thr Glu Arg Ile Ile Val Lys Gly Glu Leu Lys 
    210                 215                 220                 


Thr Glu Tyr Asp Phe Asp Lys Ala Pro Ala Asp Val Leu Lys Ala Phe 
225                 230                 235                 240 


Asn His Ser Tyr Asn Gln Val Ser Thr 
                245                 


<210>  38
<211>  1296
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Polypeptide sequence of proteolytically inactive mutant BoNT/A(0

<400>  38

Met Pro Phe Val Asn Lys Gln Phe Asn Tyr Lys Asp Pro Val Asn Gly 
1               5                   10                  15      


Val Asp Ile Ala Tyr Ile Lys Ile Pro Asn Ala Gly Gln Met Gln Pro 
            20                  25                  30          


Val Lys Ala Phe Lys Ile His Asn Lys Ile Trp Val Ile Pro Glu Arg 
        35                  40                  45              


Asp Thr Phe Thr Asn Pro Glu Glu Gly Asp Leu Asn Pro Pro Pro Glu 
    50                  55                  60                  


Ala Lys Gln Val Pro Val Ser Tyr Tyr Asp Ser Thr Tyr Leu Ser Thr 
65                  70                  75                  80  


Asp Asn Glu Lys Asp Asn Tyr Leu Lys Gly Val Thr Lys Leu Phe Glu 
                85                  90                  95      


Arg Ile Tyr Ser Thr Asp Leu Gly Arg Met Leu Leu Thr Ser Ile Val 
            100                 105                 110         


Arg Gly Ile Pro Phe Trp Gly Gly Ser Thr Ile Asp Thr Glu Leu Lys 
        115                 120                 125             


Val Ile Asp Thr Asn Cys Ile Asn Val Ile Gln Pro Asp Gly Ser Tyr 
    130                 135                 140                 


Arg Ser Glu Glu Leu Asn Leu Val Ile Ile Gly Pro Ser Ala Asp Ile 
145                 150                 155                 160 


Ile Gln Phe Glu Cys Lys Ser Phe Gly His Glu Val Leu Asn Leu Thr 
                165                 170                 175     


Arg Asn Gly Tyr Gly Ser Thr Gln Tyr Ile Arg Phe Ser Pro Asp Phe 
            180                 185                 190         


Thr Phe Gly Phe Glu Glu Ser Leu Glu Val Asp Thr Asn Pro Leu Leu 
        195                 200                 205             


Gly Ala Gly Lys Phe Ala Thr Asp Pro Ala Val Thr Leu Ala His Gln 
    210                 215                 220                 


Leu Ile Tyr Ala Gly His Arg Leu Tyr Gly Ile Ala Ile Asn Pro Asn 
225                 230                 235                 240 


Arg Val Phe Lys Val Asn Thr Asn Ala Tyr Tyr Glu Met Ser Gly Leu 
                245                 250                 255     


Glu Val Ser Phe Glu Glu Leu Arg Thr Phe Gly Gly His Asp Ala Lys 
            260                 265                 270         


Phe Ile Asp Ser Leu Gln Glu Asn Glu Phe Arg Leu Tyr Tyr Tyr Asn 
        275                 280                 285             


Lys Phe Lys Asp Ile Ala Ser Thr Leu Asn Lys Ala Lys Ser Ile Val 
    290                 295                 300                 


Gly Thr Thr Ala Ser Leu Gln Tyr Met Lys Asn Val Phe Lys Glu Lys 
305                 310                 315                 320 


Tyr Leu Leu Ser Glu Asp Thr Ser Gly Lys Phe Ser Val Asp Lys Leu 
                325                 330                 335     


Lys Phe Asp Lys Leu Tyr Lys Met Leu Thr Glu Ile Tyr Thr Glu Asp 
            340                 345                 350         


Asn Phe Val Lys Phe Phe Lys Val Leu Asn Arg Lys Thr Tyr Leu Asn 
        355                 360                 365             


Phe Asp Lys Ala Val Phe Lys Ile Asn Ile Val Pro Lys Val Asn Tyr 
    370                 375                 380                 


Thr Ile Tyr Asp Gly Phe Asn Leu Arg Asn Thr Asn Leu Ala Ala Asn 
385                 390                 395                 400 


Phe Asn Gly Gln Asn Thr Glu Ile Asn Asn Met Asn Phe Thr Lys Leu 
                405                 410                 415     


Lys Asn Phe Thr Gly Leu Phe Glu Phe Tyr Lys Leu Leu Cys Val Arg 
            420                 425                 430         


Gly Ile Ile Thr Ser Lys Thr Lys Ser Leu Asp Lys Gly Tyr Asn Lys 
        435                 440                 445             


Ala Leu Asn Asp Leu Cys Ile Lys Val Asn Asn Trp Asp Leu Phe Phe 
    450                 455                 460                 


Ser Pro Ser Glu Asp Asn Phe Thr Asn Asp Leu Asn Lys Gly Glu Glu 
465                 470                 475                 480 


Ile Thr Ser Asp Thr Asn Ile Glu Ala Ala Glu Glu Asn Ile Ser Leu 
                485                 490                 495     


Asp Leu Ile Gln Gln Tyr Tyr Leu Thr Phe Asn Phe Asp Asn Glu Pro 
            500                 505                 510         


Glu Asn Ile Ser Ile Glu Asn Leu Ser Ser Asp Ile Ile Gly Gln Leu 
        515                 520                 525             


Glu Leu Met Pro Asn Ile Glu Arg Phe Pro Asn Gly Lys Lys Tyr Glu 
    530                 535                 540                 


Leu Asp Lys Tyr Thr Met Phe His Tyr Leu Arg Ala Gln Glu Phe Glu 
545                 550                 555                 560 


His Gly Lys Ser Arg Ile Ala Leu Thr Asn Ser Val Asn Glu Ala Leu 
                565                 570                 575     


Leu Asn Pro Ser Arg Val Tyr Thr Phe Phe Ser Ser Asp Tyr Val Lys 
            580                 585                 590         


Lys Val Asn Lys Ala Thr Glu Ala Ala Met Phe Leu Gly Trp Val Glu 
        595                 600                 605             


Gln Leu Val Tyr Asp Phe Thr Asp Glu Thr Ser Glu Val Ser Thr Thr 
    610                 615                 620                 


Asp Lys Ile Ala Asp Ile Thr Ile Ile Ile Pro Tyr Ile Gly Pro Ala 
625                 630                 635                 640 


Leu Asn Ile Gly Asn Met Leu Tyr Lys Asp Asp Phe Val Gly Ala Leu 
                645                 650                 655     


Ile Phe Ser Gly Ala Val Ile Leu Leu Glu Phe Ile Pro Glu Ile Ala 
            660                 665                 670         


Ile Pro Val Leu Gly Thr Phe Ala Leu Val Ser Tyr Ile Ala Asn Lys 
        675                 680                 685             


Val Leu Thr Val Gln Thr Ile Asp Asn Ala Leu Ser Lys Arg Asn Glu 
    690                 695                 700                 


Lys Trp Asp Glu Val Tyr Lys Tyr Ile Val Thr Asn Trp Leu Ala Lys 
705                 710                 715                 720 


Val Asn Thr Gln Ile Asp Leu Ile Arg Lys Lys Met Lys Glu Ala Leu 
                725                 730                 735     


Glu Asn Gln Ala Glu Ala Thr Lys Ala Ile Ile Asn Tyr Gln Tyr Asn 
            740                 745                 750         


Gln Tyr Thr Glu Glu Glu Lys Asn Asn Ile Asn Phe Asn Ile Asp Asp 
        755                 760                 765             


Leu Ser Ser Lys Leu Asn Glu Ser Ile Asn Lys Ala Met Ile Asn Ile 
    770                 775                 780                 


Asn Lys Phe Leu Asn Gln Cys Ser Val Ser Tyr Leu Met Asn Ser Met 
785                 790                 795                 800 


Ile Pro Tyr Gly Val Lys Arg Leu Glu Asp Phe Asp Ala Ser Leu Lys 
                805                 810                 815     


Asp Ala Leu Leu Lys Tyr Ile Tyr Asp Asn Arg Gly Thr Leu Ile Gly 
            820                 825                 830         


Gln Val Asp Arg Leu Lys Asp Lys Val Asn Asn Thr Leu Ser Thr Asp 
        835                 840                 845             


Ile Pro Phe Gln Leu Ser Lys Tyr Val Asp Asn Gln Arg Leu Leu Ser 
    850                 855                 860                 


Thr Phe Thr Glu Tyr Ile Lys Asn Ile Ile Asn Thr Ser Ile Leu Asn 
865                 870                 875                 880 


Leu Arg Tyr Glu Ser Asn His Leu Ile Asp Leu Ser Arg Tyr Ala Ser 
                885                 890                 895     


Lys Ile Asn Ile Gly Ser Lys Val Asn Phe Asp Pro Ile Asp Lys Asn 
            900                 905                 910         


Gln Ile Gln Leu Phe Asn Leu Glu Ser Ser Lys Ile Glu Val Ile Leu 
        915                 920                 925             


Lys Asn Ala Ile Val Tyr Asn Ser Met Tyr Glu Asn Phe Ser Thr Ser 
    930                 935                 940                 


Phe Trp Ile Arg Ile Pro Lys Tyr Phe Asn Ser Ile Ser Leu Asn Asn 
945                 950                 955                 960 


Glu Tyr Thr Ile Ile Asn Cys Met Glu Asn Asn Ser Gly Trp Lys Val 
                965                 970                 975     


Ser Leu Asn Tyr Gly Glu Ile Ile Trp Thr Leu Gln Asp Thr Gln Glu 
            980                 985                 990         


Ile Lys Gln Arg Val Val Phe Lys  Tyr Ser Gln Met Ile  Asn Ile Ser 
        995                 1000                 1005             


Asp Tyr  Ile Asn Arg Trp Ile  Phe Val Thr Ile Thr  Asn Asn Arg 
    1010                 1015                 1020             


Leu Asn  Asn Ser Lys Ile Tyr  Ile Asn Gly Arg Leu  Ile Asp Gln 
    1025                 1030                 1035             


Lys Pro  Ile Ser Asn Leu Gly  Asn Ile His Ala Ser  Asn Asn Ile 
    1040                 1045                 1050             


Met Phe  Lys Leu Asp Gly Cys  Arg Asp Thr His Arg  Tyr Ile Trp 
    1055                 1060                 1065             


Ile Lys  Tyr Phe Asn Leu Phe  Asp Lys Glu Leu Asn  Glu Lys Glu 
    1070                 1075                 1080             


Ile Lys  Asp Leu Tyr Asp Asn  Gln Ser Asn Ser Gly  Ile Leu Lys 
    1085                 1090                 1095             


Asp Phe  Trp Gly Asp Tyr Leu  Gln Tyr Asp Lys Pro  Tyr Tyr Met 
    1100                 1105                 1110             


Leu Asn  Leu Tyr Asp Pro Asn  Lys Tyr Val Asp Val  Asn Asn Val 
    1115                 1120                 1125             


Gly Ile  Arg Gly Tyr Met Tyr  Leu Lys Gly Pro Arg  Gly Ser Val 
    1130                 1135                 1140             


Met Thr  Thr Asn Ile Tyr Leu  Asn Ser Ser Leu Tyr  Arg Gly Thr 
    1145                 1150                 1155             


Lys Phe  Ile Ile Lys Lys Tyr  Ala Ser Gly Asn Lys  Asp Asn Ile 
    1160                 1165                 1170             


Val Arg  Asn Asn Asp Arg Val  Tyr Ile Asn Val Val  Val Lys Asn 
    1175                 1180                 1185             


Lys Glu  Tyr Arg Leu Ala Thr  Asn Ala Ser Gln Ala  Gly Val Glu 
    1190                 1195                 1200             


Lys Ile  Leu Ser Ala Leu Glu  Ile Pro Asp Val Gly  Asn Leu Ser 
    1205                 1210                 1215             


Gln Val  Val Val Met Lys Ser  Lys Asn Asp Gln Gly  Ile Thr Asn 
    1220                 1225                 1230             


Lys Cys  Lys Met Asn Leu Gln  Asp Asn Asn Gly Asn  Asp Ile Gly 
    1235                 1240                 1245             


Phe Ile  Gly Phe His Gln Phe  Asn Asn Ile Ala Lys  Leu Val Ala 
    1250                 1255                 1260             


Ser Asn  Trp Tyr Asn Arg Gln  Ile Glu Arg Ser Ser  Arg Thr Leu 
    1265                 1270                 1275             


Gly Cys  Ser Trp Glu Phe Ile  Pro Val Asp Asp Gly  Trp Gly Glu 
    1280                 1285                 1290             


Arg Pro  Leu 
    1295     


<210>  39
<211>  4080
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of full length proteolytically inactive 
       mutant BoNT/A(0) with dual labelling SrtA sites

<400>  39
atggagaacc tgtattttca gggcggcggt ggcagcggcg gcagcggcgg cagcccgttt       60

gtgaacaagc agttcaacta taaagatccg gttaatggtg tggatatcgc ctatatcaaa      120

attccgaatg caggtcagat gcagccggtt aaagccttta aaatccataa caaaatttgg      180

gtgattccgg aacgtgatac ctttaccaat ccggaagaag gtgatctgaa tccgcctccg      240

gaagcaaaac aggttccggt tagctattat gatagcacct atctgagcac cgataacgag      300

aaagataact atctgaaagg tgtgaccaaa ctgtttgaac gcatttatag taccgatctg      360

ggtcgtatgc tgctgaccag cattgttcgt ggtattccgt tttggggtgg tagcaccatt      420

gataccgaac tgaaagttat tgacaccaac tgcattaatg tgattcagcc ggatggtagc      480

tatcgtagcg aagaactgaa tctggttatt attggtccga gcgcagatat cattcagttt      540

gaatgtaaaa gctttggcca cgaagttctg aatctgaccc gtaatggtta tggtagtacc      600

cagtatattc gtttcagtcc ggattttacc tttggctttg aagaaagcct ggaagttgat      660

acaaatccgc tgttaggtgc aggtaaattt gcaaccgatc cggcagttac cctggcacac      720

cagctgattt atgccggtca tcgtctgtat ggtattgcca ttaatccgaa tcgtgtgttc      780

aaagtgaata ccaacgccta ttatgaaatg agcggtctgg aagtgagttt tgaagaactg      840

cgtacctttg gtggtcatga tgccaaattt atcgatagcc tgcaagaaaa tgaatttcgc      900

ctgtactact ataacaaatt caaggatatt gcgagcaccc tgaataaagc caaaagcatt      960

gttggcacca ccgcaagcct gcagtatatg aaaaatgtgt ttaaagaaaa atatctgctg     1020

agcgaagata ccagcggtaa atttagcgtt gacaaactga aattcgataa actgtacaag     1080

atgctgaccg agatttatac cgaagataac ttcgtgaagt ttttcaaagt gctgaaccgc     1140

aaaacctacc tgaactttga taaagccgtg ttcaaaatca acatcgtgcc gaaagtgaac     1200

tataccatct atgatggttt taacctgcgc aataccaatc tggcagcaaa ctttaatggt     1260

cagaacaccg aaatcaacaa catgaacttt accaaactga agaacttcac cggtctgttc     1320

gaattttaca aactgctgtg tgttcgtggc attattacca gcaaaaccaa aagtctggat     1380

aaaggctaca ataaagccct gaatgatctg tgcattaagg tgaataattg ggacctgttt     1440

tttagcccga gcgaggataa tttcaccaac gatctgaaca aaggcgaaga aattaccagc     1500

gataccaata ttgaagcagc cgaagaaaac attagcctgg atctgattca gcagtattat     1560

ctgaccttca acttcgataa tgagccggaa aatatcagca ttgaaaacct gagcagcgat     1620

attattggcc agctggaact gatgccgaat attgaacgtt ttccgaacgg caaaaaatac     1680

gagctggata aatacaccat gttccattat ctgcgtgccc aagaatttga acatggtaaa     1740

agccgtattg cactgaccaa tagcgttaat gaagcactgc tgaacccgag ccgtgtttat     1800

acctttttta gcagcgatta cgtgaaaaag gttaacaaag caaccgaagc agccatgttt     1860

ttaggttggg ttgaacagct ggtttatgat ttcaccgatg aaaccagcga agttagcacc     1920

accgataaaa ttgcagatat taccatcatc atcccgtata tcggtccggc actgaatatt     1980

ggcaatatgc tgtataaaga cgattttgtg ggtgccctga tttttagcgg tgcagttatt     2040

ctgctggaat ttattccgga aattgccatt ccggttctgg gcacctttgc actggtgagc     2100

tatattgcaa ataaagttct gaccgtgcag accatcgata atgcactgag caaacgtaac     2160

gaaaaatggg atgaagtgta caagtatatc gtgaccaatt ggctggcaaa agttaacacc     2220

cagattgacc tgattcgcaa gaagatgaaa gaagcactgg aaaatcaggc agaagcaacc     2280

aaagccatta tcaactatca gtataaccag tacaccgaag aagagaaaaa taacatcaac     2340

ttcaacatcg acgatctgtc cagcaaactg aacgaaagca tcaacaaagc catgattaac     2400

attaacaaat ttctgaacca gtgcagcgtg agctatctga tgaatagcat gattccgtat     2460

ggtgtgaaac gtctggaaga ttttgatgca agcctgaaag atgccctgct gaaatatatc     2520

tatgataatc gtggcaccct gattggtcag gttgatcgtc tgaaagataa agtgaacaac     2580

accctgagta ccgatattcc ttttcagctg agcaaatatg tggataatca gcgtctgctg     2640

tcaaccttta ccgaatacat taagaacatc atcaacacca gcattctgaa cctgcgttat     2700

gaaagcaatc atctgattga tctgagccgt tatgccagca aaatcaatat aggcagcaag     2760

gttaacttcg acccgattga caaaaatcag atacagctgt ttaatctgga aagcagcaaa     2820

attgaggtga tcctgaaaaa cgccattgtg tataatagca tgtacgagaa tttctcgacc     2880

agcttttgga ttcgtatccc gaaatacttt aatagcatca gcctgaacaa cgagtacacc     2940

attattaact gcatggaaaa caatagcggc tggaaagtta gcctgaatta tggcgaaatt     3000

atctggaccc tgcaggatac ccaagaaatc aaacagcgtg tggttttcaa atacagccag     3060

atgattaata tcagcgacta tatcaaccgc tggatttttg tgaccattac caataatcgc     3120

ctgaataaca gcaagatcta tattaacggt cgtctgattg accagaaacc gattagtaat     3180

ctgggtaata ttcatgcgag caacaacatc atgtttaaac tggatggttg tcgtgatacc     3240

catcgttata tttggatcaa gtacttcaac ctgttcgata aagagttgaa cgaaaaagaa     3300

attaaagacc tgtatgataa ccagagcaac agcggtattc tgaaggattt ttggggagat     3360

tatctgcagt atgacaaacc gtattatatg ctgaatctgt acgacccgaa taaatacgtg     3420

gatgtgaata atgttggcat ccgtggttat atgtacctga aaggtccgcg tggtagcgtt     3480

atgaccacaa acatttatct gaatagcagc ctgtatcgcg gaaccaaatt catcattaaa     3540

aagtatgcca gcggcaacaa ggataatatt gtgcgtaata atgatcgcgt gtacattaac     3600

gttgtggtga agaataaaga atatcgcctg gcaaccaatg caagccaggc aggcgttgaa     3660

aaaattctga gtgccctgga aattccggat gttggtaatc tgagccaggt tgttgtgatg     3720

aaaagcaaaa atgatcaggg catcaccaac aagtgcaaaa tgaatctgca ggacaataac     3780

ggcaacgata ttggttttat tggcttccac cagttcaaca atattgcgaa actggttgca     3840

agcaattggt ataatcgtca gattgaacgt agcagtcgta ccctgggttg tagctgggaa     3900

tttatccctg tggatgatgg ttggggtgaa cgtccgctgg gcggcagcgg cggcggcagc     3960

ggcctgcccg aaagcggtgg cggatctgct tggtctcacc cgcagttcga aaaaggtggt     4020

ggttctggtg gtggttctgg tggttctgct tggtctcacc cgcagttcga aaaataatga     4080


<210>  40
<211>  1358
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Polypeptide sequence of full length proteolytically inactive 
       mutant BoNT/A(0) with dual labelling SrtA sites

<400>  40

Met Glu Asn Leu Tyr Phe Gln Gly Gly Gly Gly Ser Gly Gly Ser Gly 
1               5                   10                  15      


Gly Ser Pro Phe Val Asn Lys Gln Phe Asn Tyr Lys Asp Pro Val Asn 
            20                  25                  30          


Gly Val Asp Ile Ala Tyr Ile Lys Ile Pro Asn Ala Gly Gln Met Gln 
        35                  40                  45              


Pro Val Lys Ala Phe Lys Ile His Asn Lys Ile Trp Val Ile Pro Glu 
    50                  55                  60                  


Arg Asp Thr Phe Thr Asn Pro Glu Glu Gly Asp Leu Asn Pro Pro Pro 
65                  70                  75                  80  


Glu Ala Lys Gln Val Pro Val Ser Tyr Tyr Asp Ser Thr Tyr Leu Ser 
                85                  90                  95      


Thr Asp Asn Glu Lys Asp Asn Tyr Leu Lys Gly Val Thr Lys Leu Phe 
            100                 105                 110         


Glu Arg Ile Tyr Ser Thr Asp Leu Gly Arg Met Leu Leu Thr Ser Ile 
        115                 120                 125             


Val Arg Gly Ile Pro Phe Trp Gly Gly Ser Thr Ile Asp Thr Glu Leu 
    130                 135                 140                 


Lys Val Ile Asp Thr Asn Cys Ile Asn Val Ile Gln Pro Asp Gly Ser 
145                 150                 155                 160 


Tyr Arg Ser Glu Glu Leu Asn Leu Val Ile Ile Gly Pro Ser Ala Asp 
                165                 170                 175     


Ile Ile Gln Phe Glu Cys Lys Ser Phe Gly His Glu Val Leu Asn Leu 
            180                 185                 190         


Thr Arg Asn Gly Tyr Gly Ser Thr Gln Tyr Ile Arg Phe Ser Pro Asp 
        195                 200                 205             


Phe Thr Phe Gly Phe Glu Glu Ser Leu Glu Val Asp Thr Asn Pro Leu 
    210                 215                 220                 


Leu Gly Ala Gly Lys Phe Ala Thr Asp Pro Ala Val Thr Leu Ala His 
225                 230                 235                 240 


Gln Leu Ile Tyr Ala Gly His Arg Leu Tyr Gly Ile Ala Ile Asn Pro 
                245                 250                 255     


Asn Arg Val Phe Lys Val Asn Thr Asn Ala Tyr Tyr Glu Met Ser Gly 
            260                 265                 270         


Leu Glu Val Ser Phe Glu Glu Leu Arg Thr Phe Gly Gly His Asp Ala 
        275                 280                 285             


Lys Phe Ile Asp Ser Leu Gln Glu Asn Glu Phe Arg Leu Tyr Tyr Tyr 
    290                 295                 300                 


Asn Lys Phe Lys Asp Ile Ala Ser Thr Leu Asn Lys Ala Lys Ser Ile 
305                 310                 315                 320 


Val Gly Thr Thr Ala Ser Leu Gln Tyr Met Lys Asn Val Phe Lys Glu 
                325                 330                 335     


Lys Tyr Leu Leu Ser Glu Asp Thr Ser Gly Lys Phe Ser Val Asp Lys 
            340                 345                 350         


Leu Lys Phe Asp Lys Leu Tyr Lys Met Leu Thr Glu Ile Tyr Thr Glu 
        355                 360                 365             


Asp Asn Phe Val Lys Phe Phe Lys Val Leu Asn Arg Lys Thr Tyr Leu 
    370                 375                 380                 


Asn Phe Asp Lys Ala Val Phe Lys Ile Asn Ile Val Pro Lys Val Asn 
385                 390                 395                 400 


Tyr Thr Ile Tyr Asp Gly Phe Asn Leu Arg Asn Thr Asn Leu Ala Ala 
                405                 410                 415     


Asn Phe Asn Gly Gln Asn Thr Glu Ile Asn Asn Met Asn Phe Thr Lys 
            420                 425                 430         


Leu Lys Asn Phe Thr Gly Leu Phe Glu Phe Tyr Lys Leu Leu Cys Val 
        435                 440                 445             


Arg Gly Ile Ile Thr Ser Lys Thr Lys Ser Leu Asp Lys Gly Tyr Asn 
    450                 455                 460                 


Lys Ala Leu Asn Asp Leu Cys Ile Lys Val Asn Asn Trp Asp Leu Phe 
465                 470                 475                 480 


Phe Ser Pro Ser Glu Asp Asn Phe Thr Asn Asp Leu Asn Lys Gly Glu 
                485                 490                 495     


Glu Ile Thr Ser Asp Thr Asn Ile Glu Ala Ala Glu Glu Asn Ile Ser 
            500                 505                 510         


Leu Asp Leu Ile Gln Gln Tyr Tyr Leu Thr Phe Asn Phe Asp Asn Glu 
        515                 520                 525             


Pro Glu Asn Ile Ser Ile Glu Asn Leu Ser Ser Asp Ile Ile Gly Gln 
    530                 535                 540                 


Leu Glu Leu Met Pro Asn Ile Glu Arg Phe Pro Asn Gly Lys Lys Tyr 
545                 550                 555                 560 


Glu Leu Asp Lys Tyr Thr Met Phe His Tyr Leu Arg Ala Gln Glu Phe 
                565                 570                 575     


Glu His Gly Lys Ser Arg Ile Ala Leu Thr Asn Ser Val Asn Glu Ala 
            580                 585                 590         


Leu Leu Asn Pro Ser Arg Val Tyr Thr Phe Phe Ser Ser Asp Tyr Val 
        595                 600                 605             


Lys Lys Val Asn Lys Ala Thr Glu Ala Ala Met Phe Leu Gly Trp Val 
    610                 615                 620                 


Glu Gln Leu Val Tyr Asp Phe Thr Asp Glu Thr Ser Glu Val Ser Thr 
625                 630                 635                 640 


Thr Asp Lys Ile Ala Asp Ile Thr Ile Ile Ile Pro Tyr Ile Gly Pro 
                645                 650                 655     


Ala Leu Asn Ile Gly Asn Met Leu Tyr Lys Asp Asp Phe Val Gly Ala 
            660                 665                 670         


Leu Ile Phe Ser Gly Ala Val Ile Leu Leu Glu Phe Ile Pro Glu Ile 
        675                 680                 685             


Ala Ile Pro Val Leu Gly Thr Phe Ala Leu Val Ser Tyr Ile Ala Asn 
    690                 695                 700                 


Lys Val Leu Thr Val Gln Thr Ile Asp Asn Ala Leu Ser Lys Arg Asn 
705                 710                 715                 720 


Glu Lys Trp Asp Glu Val Tyr Lys Tyr Ile Val Thr Asn Trp Leu Ala 
                725                 730                 735     


Lys Val Asn Thr Gln Ile Asp Leu Ile Arg Lys Lys Met Lys Glu Ala 
            740                 745                 750         


Leu Glu Asn Gln Ala Glu Ala Thr Lys Ala Ile Ile Asn Tyr Gln Tyr 
        755                 760                 765             


Asn Gln Tyr Thr Glu Glu Glu Lys Asn Asn Ile Asn Phe Asn Ile Asp 
    770                 775                 780                 


Asp Leu Ser Ser Lys Leu Asn Glu Ser Ile Asn Lys Ala Met Ile Asn 
785                 790                 795                 800 


Ile Asn Lys Phe Leu Asn Gln Cys Ser Val Ser Tyr Leu Met Asn Ser 
                805                 810                 815     


Met Ile Pro Tyr Gly Val Lys Arg Leu Glu Asp Phe Asp Ala Ser Leu 
            820                 825                 830         


Lys Asp Ala Leu Leu Lys Tyr Ile Tyr Asp Asn Arg Gly Thr Leu Ile 
        835                 840                 845             


Gly Gln Val Asp Arg Leu Lys Asp Lys Val Asn Asn Thr Leu Ser Thr 
    850                 855                 860                 


Asp Ile Pro Phe Gln Leu Ser Lys Tyr Val Asp Asn Gln Arg Leu Leu 
865                 870                 875                 880 


Ser Thr Phe Thr Glu Tyr Ile Lys Asn Ile Ile Asn Thr Ser Ile Leu 
                885                 890                 895     


Asn Leu Arg Tyr Glu Ser Asn His Leu Ile Asp Leu Ser Arg Tyr Ala 
            900                 905                 910         


Ser Lys Ile Asn Ile Gly Ser Lys Val Asn Phe Asp Pro Ile Asp Lys 
        915                 920                 925             


Asn Gln Ile Gln Leu Phe Asn Leu Glu Ser Ser Lys Ile Glu Val Ile 
    930                 935                 940                 


Leu Lys Asn Ala Ile Val Tyr Asn Ser Met Tyr Glu Asn Phe Ser Thr 
945                 950                 955                 960 


Ser Phe Trp Ile Arg Ile Pro Lys Tyr Phe Asn Ser Ile Ser Leu Asn 
                965                 970                 975     


Asn Glu Tyr Thr Ile Ile Asn Cys Met Glu Asn Asn Ser Gly Trp Lys 
            980                 985                 990         


Val Ser Leu Asn Tyr Gly Glu Ile  Ile Trp Thr Leu Gln  Asp Thr Gln 
        995                 1000                 1005             


Glu Ile  Lys Gln Arg Val Val  Phe Lys Tyr Ser Gln  Met Ile Asn 
    1010                 1015                 1020             


Ile Ser  Asp Tyr Ile Asn Arg  Trp Ile Phe Val Thr  Ile Thr Asn 
    1025                 1030                 1035             


Asn Arg  Leu Asn Asn Ser Lys  Ile Tyr Ile Asn Gly  Arg Leu Ile 
    1040                 1045                 1050             


Asp Gln  Lys Pro Ile Ser Asn  Leu Gly Asn Ile His  Ala Ser Asn 
    1055                 1060                 1065             


Asn Ile  Met Phe Lys Leu Asp  Gly Cys Arg Asp Thr  His Arg Tyr 
    1070                 1075                 1080             


Ile Trp  Ile Lys Tyr Phe Asn  Leu Phe Asp Lys Glu  Leu Asn Glu 
    1085                 1090                 1095             


Lys Glu  Ile Lys Asp Leu Tyr  Asp Asn Gln Ser Asn  Ser Gly Ile 
    1100                 1105                 1110             


Leu Lys  Asp Phe Trp Gly Asp  Tyr Leu Gln Tyr Asp  Lys Pro Tyr 
    1115                 1120                 1125             


Tyr Met  Leu Asn Leu Tyr Asp  Pro Asn Lys Tyr Val  Asp Val Asn 
    1130                 1135                 1140             


Asn Val  Gly Ile Arg Gly Tyr  Met Tyr Leu Lys Gly  Pro Arg Gly 
    1145                 1150                 1155             


Ser Val  Met Thr Thr Asn Ile  Tyr Leu Asn Ser Ser  Leu Tyr Arg 
    1160                 1165                 1170             


Gly Thr  Lys Phe Ile Ile Lys  Lys Tyr Ala Ser Gly  Asn Lys Asp 
    1175                 1180                 1185             


Asn Ile  Val Arg Asn Asn Asp  Arg Val Tyr Ile Asn  Val Val Val 
    1190                 1195                 1200             


Lys Asn  Lys Glu Tyr Arg Leu  Ala Thr Asn Ala Ser  Gln Ala Gly 
    1205                 1210                 1215             


Val Glu  Lys Ile Leu Ser Ala  Leu Glu Ile Pro Asp  Val Gly Asn 
    1220                 1225                 1230             


Leu Ser  Gln Val Val Val Met  Lys Ser Lys Asn Asp  Gln Gly Ile 
    1235                 1240                 1245             


Thr Asn  Lys Cys Lys Met Asn  Leu Gln Asp Asn Asn  Gly Asn Asp 
    1250                 1255                 1260             


Ile Gly  Phe Ile Gly Phe His  Gln Phe Asn Asn Ile  Ala Lys Leu 
    1265                 1270                 1275             


Val Ala  Ser Asn Trp Tyr Asn  Arg Gln Ile Glu Arg  Ser Ser Arg 
    1280                 1285                 1290             


Thr Leu  Gly Cys Ser Trp Glu  Phe Ile Pro Val Asp  Asp Gly Trp 
    1295                 1300                 1305             


Gly Glu  Arg Pro Leu Gly Gly  Ser Gly Gly Gly Ser  Gly Leu Pro 
    1310                 1315                 1320             


Glu Ser  Gly Gly Gly Ser Ala  Trp Ser His Pro Gln  Phe Glu Lys 
    1325                 1330                 1335             


Gly Gly  Gly Ser Gly Gly Gly  Ser Gly Gly Ser Ala  Trp Ser His 
    1340                 1345                 1350             


Pro Gln  Phe Glu Lys 
    1355             


<210>  41
<211>  1191
<212>  PRT
<213>  Prochloron didemni

<400>  41

Met Phe Ser Ile Met Ile Thr Ile Asp Tyr Pro Phe Thr Val Ser Leu 
1               5                   10                  15      


Asn Arg Asp Ile Gln Val Thr Ser Thr Glu Asp Tyr Tyr Thr Leu Gln 
            20                  25                  30          


Val Thr Glu Ser Asp Pro Ser Ala Trp Leu Thr Phe Ala Thr Thr Pro 
        35                  40                  45              


Ala Met Asp Met Ala Phe Asp His Leu Lys Ala Gly Thr Thr Thr Glu 
    50                  55                  60                  


Ser Leu Val Gln Thr Leu Ala Glu Leu Gly Gly Pro Ala Ala Arg Glu 
65                  70                  75                  80  


Gln Phe Ala Leu Thr Leu Gln Gln Leu Asp Glu Arg Gly Trp Leu Ser 
                85                  90                  95      


Tyr Ala Val Leu Pro Leu Ala Glu Ala Ile Pro Met Val Glu Ser Ala 
            100                 105                 110         


Glu Leu Asn Leu Pro Gly Asn Pro His Trp Met Glu Thr Gly Val Thr 
        115                 120                 125             


Leu Ser Arg Phe Ala Tyr Gln His Pro Tyr Glu Gly Thr Met Val Leu 
    130                 135                 140                 


Glu Ser Pro Leu Ser Lys Phe Arg Val Lys Leu Leu Asp Trp Arg Ala 
145                 150                 155                 160 


Ser Ala Leu Leu Ala Gln Leu Ala Gln Pro Gln Thr Leu Gly Thr Ile 
                165                 170                 175     


Ala Pro Pro Pro Tyr Leu Gly Pro Glu Thr Ala Tyr Gln Phe Leu Asn 
            180                 185                 190         


Leu Leu Trp Ala Thr Gly Phe Leu Ala Ser Asp His Glu Pro Val Ser 
        195                 200                 205             


Leu Gln Leu Trp Asp Phe His Asn Leu Leu Phe His Ser Arg Ser Arg 
    210                 215                 220                 


Leu Gly Arg His Asp Tyr Pro Gly Thr Asp Leu Asn Val Asp Asn Trp 
225                 230                 235                 240 


Ser Asp Phe Pro Val Val Lys Pro Pro Met Ser Asp Arg Ile Val Pro 
                245                 250                 255     


Leu Pro Arg Pro Asn Leu Glu Ala Leu Met Ser Asn Asp Ala Thr Leu 
            260                 265                 270         


Thr Glu Ala Ile Glu Thr Arg Lys Ser Val Arg Glu Tyr Asp Asp Asp 
        275                 280                 285             


Asn Pro Ile Thr Ile Glu Gln Leu Gly Glu Leu Leu Tyr Arg Ala Ala 
    290                 295                 300                 


Arg Val Thr Lys Leu Leu Ser Pro Glu Glu Arg Phe Gly Lys Leu Trp 
305                 310                 315                 320 


Gln Gln Asn Lys Pro Val Phe Glu Glu Ala Gly Val Asp Glu Gly Glu 
                325                 330                 335     


Phe Ser His Arg Pro Tyr Pro Gly Gly Gly Ala Met Tyr Glu Leu Glu 
            340                 345                 350         


Ile Tyr Pro Val Val Arg Leu Cys Gln Gly Leu Ser Gln Gly Val Tyr 
        355                 360                 365             


His Tyr Asp Pro Leu Asn His Gln Leu Glu Gln Ile Val Glu Ser Lys 
    370                 375                 380                 


Asp Asp Ile Phe Ala Val Ser Gly Ser Pro Leu Ala Ser Lys Leu Gly 
385                 390                 395                 400 


Pro His Val Leu Leu Val Ile Thr Ala Arg Phe Gly Arg Leu Phe Arg 
                405                 410                 415     


Leu Tyr Arg Ser Val Ala Tyr Ala Leu Val Leu Lys His Val Gly Val 
            420                 425                 430         


Leu Gln Gln Asn Leu Tyr Leu Val Ala Thr Asn Met Gly Leu Ala Pro 
        435                 440                 445             


Cys Ala Gly Gly Ala Gly Asp Ser Asp Ala Phe Ala Gln Val Thr Gly 
    450                 455                 460                 


Ile Asp Tyr Val Glu Glu Ser Ala Val Gly Glu Phe Ile Leu Gly Ser 
465                 470                 475                 480 


Leu Ala Ser Glu Val Glu Ser Asp Val Val Glu Gly Glu Asp Glu Ile 
                485                 490                 495     


Glu Ser Ala Gly Val Ser Ala Ser Glu Val Glu Ser Ser Ala Thr Lys 
            500                 505                 510         


Gln Lys Val Ala Leu His Pro His Asp Leu Asp Glu Arg Ile Pro Gly 
        515                 520                 525             


Leu Ala Asp Leu His Asn Gln Thr Leu Gly Asp Pro Gln Ile Thr Ile 
    530                 535                 540                 


Val Ile Ile Asp Gly Asp Pro Asp Tyr Thr Leu Ser Cys Phe Glu Gly 
545                 550                 555                 560 


Ala Glu Val Ser Lys Val Phe Pro Tyr Trp His Glu Pro Ala Glu Pro 
                565                 570                 575     


Ile Thr Pro Glu Asp Tyr Ala Ala Phe Gln Ser Ile Arg Asp Gln Gly 
            580                 585                 590         


Leu Lys Gly Lys Glu Lys Glu Glu Ala Leu Glu Ala Val Ile Pro Asp 
        595                 600                 605             


Thr Lys Asp Arg Ile Val Leu Asn Asp His Ala Cys His Val Thr Ser 
    610                 615                 620                 


Thr Ile Val Gly Gln Glu His Ser Pro Val Phe Gly Ile Ala Pro Asn 
625                 630                 635                 640 


Cys Arg Val Ile Asn Met Pro Gln Asp Ala Val Ile Arg Gly Asn Tyr 
                645                 650                 655     


Asp Asp Val Met Ser Pro Leu Asn Leu Ala Arg Ala Ile Asp Leu Ala 
            660                 665                 670         


Leu Glu Leu Gly Ala Asn Ile Ile His Cys Ala Phe Cys Arg Pro Thr 
        675                 680                 685             


Gln Thr Ser Glu Gly Glu Glu Ile Leu Val Gln Ala Ile Lys Lys Cys 
    690                 695                 700                 


Gln Asp Asn Asn Val Leu Ile Val Ser Pro Thr Gly Asn Asn Ser Asn 
705                 710                 715                 720 


Glu Ser Trp Cys Leu Pro Ala Val Leu Pro Gly Thr Leu Ala Val Gly 
                725                 730                 735     


Ala Ala Lys Val Asp Gly Thr Pro Cys His Phe Ser Asn Trp Gly Gly 
            740                 745                 750         


Asn Asn Thr Lys Glu Gly Ile Leu Ala Pro Gly Glu Glu Ile Leu Gly 
        755                 760                 765             


Ala Gln Pro Cys Thr Glu Glu Pro Val Arg Leu Thr Gly Thr Ser Met 
    770                 775                 780                 


Ala Ala Pro Val Met Thr Gly Ile Ser Ala Leu Leu Met Ser Leu Gln 
785                 790                 795                 800 


Val Gln Gln Gly Lys Pro Val Asp Ala Glu Ala Val Arg Thr Ala Leu 
                805                 810                 815     


Leu Lys Thr Ala Ile Pro Cys Asp Pro Glu Val Val Glu Glu Pro Glu 
            820                 825                 830         


Arg Cys Leu Arg Gly Phe Val Asn Ile Pro Gly Ala Met Lys Val Leu 
        835                 840                 845             


Phe Gly Gln Pro Ser Val Thr Val Ser Phe Ala Gly Gly Gln Ala Thr 
    850                 855                 860                 


Arg Thr Glu His Pro Gly Tyr Ala Thr Val Ala Pro Ala Ser Ile Pro 
865                 870                 875                 880 


Glu Pro Met Ala Glu Arg Ala Thr Pro Ala Val Gln Ala Ala Thr Ala 
                885                 890                 895     


Thr Glu Met Val Ile Ala Pro Ser Thr Glu Pro Ala Asn Pro Ala Thr 
            900                 905                 910         


Val Glu Ala Ser Thr Ala Phe Ser Gly Asn Val Tyr Ala Leu Gly Thr 
        915                 920                 925             


Ile Gly Tyr Asp Phe Gly Asp Glu Ala Arg Arg Asp Thr Phe Lys Glu 
    930                 935                 940                 


Arg Met Ala Asp Pro Tyr Asp Ala Arg Gln Met Val Asp Tyr Leu Asp 
945                 950                 955                 960 


Arg Asn Pro Asp Glu Ala Arg Ser Leu Ile Trp Thr Leu Asn Leu Glu 
                965                 970                 975     


Gly Asp Val Ile Tyr Ala Leu Asp Pro Lys Gly Pro Phe Ala Thr Asn 
            980                 985                 990         


Val Tyr Glu Ile Phe Leu Gln Met  Leu Ala Gly Gln Leu  Glu Pro Glu 
        995                 1000                 1005             


Thr Ser  Ala Asp Phe Ile Glu  Arg Leu Ser Val Pro  Ala Arg Arg 
    1010                 1015                 1020             


Thr Thr  Arg Thr Val Glu Leu  Phe Ser Gly Glu Val  Met Pro Val 
    1025                 1030                 1035             


Val Asn  Val Arg Asp Pro Arg  Gly Met Tyr Gly Trp  Asn Val Asn 
    1040                 1045                 1050             


Ala Leu  Val Asp Ala Ala Leu  Ala Thr Val Glu Tyr  Glu Glu Ala 
    1055                 1060                 1065             


Asp Glu  Asp Ser Leu Arg Gln  Gly Leu Thr Ala Phe  Leu Asn Arg 
    1070                 1075                 1080             


Val Tyr  His Asp Leu His Asn  Leu Gly Gln Thr Ser  Arg Asp Arg 
    1085                 1090                 1095             


Ala Leu  Asn Phe Thr Val Thr  Asn Thr Phe Gln Ala  Ala Ser Thr 
    1100                 1105                 1110             


Phe Ala  Gln Ala Ile Ala Ser  Gly Arg Gln Leu Asp  Thr Ile Glu 
    1115                 1120                 1125             


Val Asn  Lys Ser Pro Tyr Cys  Arg Leu Asn Ser Asp  Cys Trp Asp 
    1130                 1135                 1140             


Val Leu  Leu Thr Phe Tyr Asp  Pro Glu His Gly Arg  Arg Ser Arg 
    1145                 1150                 1155             


Arg Val  Phe Arg Phe Thr Leu  Asp Val Val Tyr Val  Leu Pro Val 
    1160                 1165                 1170             


Thr Val  Gly Ser Ile Lys Ser  Trp Ser Leu Pro Gly  Lys Gly Thr 
    1175                 1180                 1185             


Val Ser  Lys 
    1190     


<210>  42
<211>  724
<212>  PRT
<213>  Saponaria vaccaria

<400>  42

Met Ala Thr Ser Gly Phe Ser Lys Pro Leu His Tyr Pro Pro Val Arg 
1               5                   10                  15      


Arg Asp Glu Thr Val Val Asp Asp Tyr Phe Gly Val Lys Val Ala Asp 
            20                  25                  30          


Pro Tyr Arg Trp Leu Glu Asp Pro Asn Ser Glu Glu Thr Lys Glu Phe 
        35                  40                  45              


Val Asp Asn Gln Glu Lys Leu Ala Asn Ser Val Leu Glu Glu Cys Glu 
    50                  55                  60                  


Leu Ile Asp Lys Phe Lys Gln Lys Ile Ile Asp Phe Val Asn Phe Pro 
65                  70                  75                  80  


Arg Cys Gly Val Pro Phe Arg Arg Ala Asn Lys Tyr Phe His Phe Tyr 
                85                  90                  95      


Asn Ser Gly Leu Gln Ala Gln Asn Val Phe Gln Met Gln Asp Asp Leu 
            100                 105                 110         


Asp Gly Lys Pro Glu Val Leu Tyr Asp Pro Asn Leu Arg Glu Gly Gly 
        115                 120                 125             


Arg Ser Gly Leu Ser Leu Tyr Ser Val Ser Glu Asp Ala Lys Tyr Phe 
    130                 135                 140                 


Ala Phe Gly Ile His Ser Gly Leu Thr Glu Trp Val Thr Ile Lys Ile 
145                 150                 155                 160 


Leu Lys Thr Glu Asp Arg Ser Tyr Leu Pro Asp Thr Leu Glu Trp Val 
                165                 170                 175     


Lys Phe Ser Pro Ala Ile Trp Thr His Asp Asn Lys Gly Phe Phe Tyr 
            180                 185                 190         


Cys Pro Tyr Pro Pro Leu Lys Glu Gly Glu Asp His Met Thr Arg Ser 
        195                 200                 205             


Ala Val Asn Gln Glu Ala Arg Tyr His Phe Leu Gly Thr Asp Gln Ser 
    210                 215                 220                 


Glu Asp Ile Leu Leu Trp Arg Asp Leu Glu Asn Pro Ala His His Leu 
225                 230                 235                 240 


Lys Cys Gln Ile Thr Asp Asp Gly Lys Tyr Phe Leu Leu Tyr Ile Leu 
                245                 250                 255     


Asp Gly Cys Asp Asp Ala Asn Lys Val Tyr Cys Leu Asp Leu Thr Lys 
            260                 265                 270         


Leu Pro Asn Gly Leu Glu Ser Phe Arg Gly Arg Glu Asp Ser Ala Pro 
        275                 280                 285             


Phe Met Lys Leu Ile Asp Ser Phe Asp Ala Ser Tyr Thr Ala Ile Ala 
    290                 295                 300                 


Asn Asp Gly Ser Val Phe Thr Phe Gln Thr Asn Lys Asp Ala Pro Arg 
305                 310                 315                 320 


Lys Lys Leu Val Arg Val Asp Leu Asn Asn Pro Ser Val Trp Thr Asp 
                325                 330                 335     


Leu Val Pro Glu Ser Lys Lys Asp Leu Leu Glu Ser Ala His Ala Val 
            340                 345                 350         


Asn Glu Asn Gln Leu Ile Leu Arg Tyr Leu Ser Asp Val Lys His Val 
        355                 360                 365             


Leu Glu Ile Arg Asp Leu Glu Ser Gly Ala Leu Gln His Arg Leu Pro 
    370                 375                 380                 


Ile Asp Ile Gly Ser Val Asp Gly Ile Thr Ala Arg Arg Arg Asp Ser 
385                 390                 395                 400 


Val Val Phe Phe Lys Phe Thr Ser Ile Leu Thr Pro Gly Ile Val Tyr 
                405                 410                 415     


Gln Cys Asp Leu Lys Asn Asp Pro Thr Gln Leu Lys Ile Phe Arg Glu 
            420                 425                 430         


Ser Val Val Pro Asp Phe Asp Arg Ser Glu Phe Glu Val Lys Gln Val 
        435                 440                 445             


Phe Val Pro Ser Lys Asp Gly Thr Lys Ile Pro Ile Phe Ile Ala Ala 
    450                 455                 460                 


Arg Lys Gly Ile Ser Leu Asp Gly Ser His Pro Cys Glu Met His Gly 
465                 470                 475                 480 


Tyr Gly Gly Phe Gly Ile Asn Met Met Pro Thr Phe Ser Ala Ser Arg 
                485                 490                 495     


Ile Val Phe Leu Lys His Leu Gly Gly Val Phe Cys Leu Ala Asn Ile 
            500                 505                 510         


Arg Gly Gly Gly Glu Tyr Gly Glu Glu Trp His Lys Ala Gly Phe Arg 
        515                 520                 525             


Asp Lys Lys Gln Asn Val Phe Asp Asp Phe Ile Ser Ala Ala Glu Tyr 
    530                 535                 540                 


Leu Ile Ser Ser Gly Tyr Thr Lys Ala Arg Arg Val Ala Ile Glu Gly 
545                 550                 555                 560 


Gly Ser Asn Gly Gly Leu Leu Val Ala Ala Cys Ile Asn Gln Arg Pro 
                565                 570                 575     


Asp Leu Phe Gly Cys Ala Glu Ala Asn Cys Gly Val Met Asp Met Leu 
            580                 585                 590         


Arg Phe His Lys Phe Thr Leu Gly Tyr Leu Trp Thr Gly Asp Tyr Gly 
        595                 600                 605             


Cys Ser Asp Lys Glu Glu Glu Phe Lys Trp Leu Ile Lys Tyr Ser Pro 
    610                 615                 620                 


Ile His Asn Val Arg Arg Pro Trp Glu Gln Pro Gly Asn Glu Glu Thr 
625                 630                 635                 640 


Gln Tyr Pro Ala Thr Met Ile Leu Thr Ala Asp His Asp Asp Arg Val 
                645                 650                 655     


Val Pro Leu His Ser Phe Lys Leu Leu Ala Thr Met Gln His Val Leu 
            660                 665                 670         


Cys Thr Ser Leu Glu Asp Ser Pro Gln Lys Asn Pro Ile Ile Ala Arg 
        675                 680                 685             


Ile Gln Arg Lys Ala Ala His Tyr Gly Arg Ala Thr Met Thr Gln Ile 
    690                 695                 700                 


Ala Glu Val Ala Asp Arg Tyr Gly Phe Met Ala Lys Ala Leu Glu Ala 
705                 710                 715                 720 


Pro Trp Ile Asp 
                


<210>  43
<211>  730
<212>  PRT
<213>  Galerina marginata

<400>  43

Met Ser Ser Val Thr Trp Ala Pro Gly Asn Tyr Pro Ser Thr Arg Arg 
1               5                   10                  15      


Ser Asp His Val Asp Thr Tyr Gln Ser Ala Ser Lys Gly Glu Val Pro 
            20                  25                  30          


Val Pro Asp Pro Tyr Gln Trp Leu Glu Glu Ser Thr Asp Glu Val Asp 
        35                  40                  45              


Lys Trp Thr Thr Ala Gln Ala Asp Leu Ala Gln Ser Tyr Leu Asp Gln 
    50                  55                  60                  


Asn Ala Asp Ile Gln Lys Leu Ala Glu Lys Phe Arg Ala Ser Arg Asn 
65                  70                  75                  80  


Tyr Ala Lys Phe Ser Ala Pro Thr Leu Leu Asp Asp Gly His Trp Tyr 
                85                  90                  95      


Trp Phe Tyr Asn Arg Gly Leu Gln Ser Gln Ser Val Leu Tyr Arg Ser 
            100                 105                 110         


Lys Glu Pro Ala Leu Pro Asp Phe Ser Lys Gly Asp Asp Asn Val Gly 
        115                 120                 125             


Asp Val Phe Phe Asp Pro Asn Val Leu Ala Ala Asp Gly Ser Ala Gly 
    130                 135                 140                 


Met Val Leu Cys Lys Phe Ser Pro Asp Gly Lys Phe Phe Ala Tyr Ala 
145                 150                 155                 160 


Val Ser His Leu Gly Gly Asp Tyr Ser Thr Ile Tyr Val Arg Ser Thr 
                165                 170                 175     


Ser Ser Pro Leu Ser Gln Ala Ser Val Ala Gln Gly Val Asp Gly Arg 
            180                 185                 190         


Leu Ser Asp Glu Val Lys Trp Phe Lys Phe Ser Thr Ile Ile Trp Thr 
        195                 200                 205             


Lys Asp Ser Lys Gly Phe Leu Tyr Gln Arg Tyr Pro Ala Arg Glu Arg 
    210                 215                 220                 


His Glu Gly Thr Arg Ser Asp Arg Asn Ala Met Met Cys Tyr His Lys 
225                 230                 235                 240 


Val Gly Thr Thr Gln Glu Glu Asp Ile Ile Val Tyr Gln Asp Asn Glu 
                245                 250                 255     


His Pro Glu Trp Ile Tyr Gly Ala Asp Thr Ser Glu Asp Gly Lys Tyr 
            260                 265                 270         


Leu Tyr Leu Tyr Gln Phe Lys Asp Thr Ser Lys Lys Asn Leu Leu Trp 
        275                 280                 285             


Val Ala Glu Leu Asp Glu Asp Gly Val Lys Ser Gly Ile His Trp Arg 
    290                 295                 300                 


Lys Val Val Asn Glu Tyr Ala Ala Asp Tyr Asn Ile Ile Thr Asn His 
305                 310                 315                 320 


Gly Ser Leu Val Tyr Ile Lys Thr Asn Leu Asn Ala Pro Gln Tyr Lys 
                325                 330                 335     


Val Ile Thr Ile Asp Leu Ser Lys Asp Glu Pro Glu Ile Arg Asp Phe 
            340                 345                 350         


Ile Pro Glu Glu Lys Asp Ala Lys Leu Ala Gln Val Asn Cys Ala Asn 
        355                 360                 365             


Glu Glu Tyr Phe Val Ala Ile Tyr Lys Arg Asn Val Lys Asp Glu Ile 
    370                 375                 380                 


Tyr Leu Tyr Ser Lys Ala Gly Val Gln Leu Thr Arg Leu Ala Pro Asp 
385                 390                 395                 400 


Phe Val Gly Ala Ala Ser Ile Ala Asn Arg Gln Lys Gln Thr His Phe 
                405                 410                 415     


Phe Leu Thr Leu Ser Gly Phe Asn Thr Pro Gly Thr Ile Ala Arg Tyr 
            420                 425                 430         


Asp Phe Thr Ala Pro Glu Thr Gln Arg Phe Ser Ile Leu Arg Thr Thr 
        435                 440                 445             


Lys Val Asn Glu Leu Asp Pro Asp Asp Phe Glu Ser Thr Gln Val Trp 
    450                 455                 460                 


Tyr Glu Ser Lys Asp Gly Thr Lys Ile Pro Met Phe Ile Val Arg His 
465                 470                 475                 480 


Lys Ser Thr Lys Phe Asp Gly Thr Ala Ala Ala Ile Gln Tyr Gly Tyr 
                485                 490                 495     


Gly Gly Phe Ala Thr Ser Ala Asp Pro Phe Phe Ser Pro Ile Ile Leu 
            500                 505                 510         


Thr Phe Leu Gln Thr Tyr Gly Ala Ile Phe Ala Val Pro Ser Ile Arg 
        515                 520                 525             


Gly Gly Gly Glu Phe Gly Glu Glu Trp His Lys Gly Gly Arg Arg Glu 
    530                 535                 540                 


Thr Lys Val Asn Thr Phe Asp Asp Phe Ile Ala Ala Ala Gln Phe Leu 
545                 550                 555                 560 


Val Lys Asn Lys Tyr Ala Ala Pro Gly Lys Val Ala Ile Asn Gly Ala 
                565                 570                 575     


Ser Asn Gly Gly Leu Leu Val Met Gly Ser Ile Val Arg Ala Pro Glu 
            580                 585                 590         


Gly Thr Phe Gly Ala Ala Val Pro Glu Gly Gly Val Ala Asp Leu Leu 
        595                 600                 605             


Lys Phe His Lys Phe Thr Gly Gly Gln Ala Trp Ile Ser Glu Tyr Gly 
    610                 615                 620                 


Asn Pro Ser Ile Pro Glu Glu Phe Asp Tyr Ile Tyr Pro Leu Ser Pro 
625                 630                 635                 640 


Val His Asn Val Arg Thr Asp Lys Val Met Pro Ala Thr Leu Ile Thr 
                645                 650                 655     


Val Asn Ile Gly Asp Gly Arg Val Val Pro Met His Ser Phe Lys Phe 
            660                 665                 670         


Ile Ala Thr Leu Gln His Asn Val Pro Gln Asn Pro His Pro Leu Leu 
        675                 680                 685             


Ile Lys Ile Asp Lys Ser Trp Leu Gly His Gly Met Gly Lys Pro Thr 
    690                 695                 700                 


Asp Lys Asn Val Lys Asp Ala Ala Asp Lys Trp Gly Phe Ile Ala Arg 
705                 710                 715                 720 


Ala Leu Gly Leu Glu Leu Lys Thr Val Glu 
                725                 730 


<210>  44
<211>  474
<212>  PRT
<213>  Oldenlandia affinis

<400>  44

Met Val Arg Tyr Leu Ala Gly Ala Val Leu Leu Leu Val Val Leu Ser 
1               5                   10                  15      


Val Ala Ala Ala Val Ser Gly Ala Arg Asp Gly Asp Tyr Leu His Leu 
            20                  25                  30          


Pro Ser Glu Val Ser Arg Phe Phe Arg Pro Gln Glu Thr Asn Asp Asp 
        35                  40                  45              


His Gly Glu Asp Ser Val Gly Thr Arg Trp Ala Val Leu Ile Ala Gly 
    50                  55                  60                  


Ser Lys Gly Tyr Ala Asn Tyr Arg His Gln Ala Gly Val Cys His Ala 
65                  70                  75                  80  


Tyr Gln Ile Leu Lys Arg Gly Gly Leu Lys Asp Glu Asn Ile Val Val 
                85                  90                  95      


Phe Met Tyr Asp Asp Ile Ala Tyr Asn Glu Ser Asn Pro Arg Pro Gly 
            100                 105                 110         


Val Ile Ile Asn Ser Pro His Gly Ser Asp Val Tyr Ala Gly Val Pro 
        115                 120                 125             


Lys Asp Tyr Thr Gly Glu Glu Val Asn Ala Lys Asn Phe Leu Ala Ala 
    130                 135                 140                 


Ile Leu Gly Asn Lys Ser Ala Ile Thr Gly Gly Ser Gly Lys Val Val 
145                 150                 155                 160 


Asp Ser Gly Pro Asn Asp His Ile Phe Ile Tyr Tyr Thr Asp His Gly 
                165                 170                 175     


Ala Ala Gly Val Ile Gly Met Pro Ser Lys Pro Tyr Leu Tyr Ala Asp 
            180                 185                 190         


Glu Leu Asn Asp Ala Leu Lys Lys Lys His Ala Ser Gly Thr Tyr Lys 
        195                 200                 205             


Ser Leu Val Phe Tyr Leu Glu Ala Cys Glu Ser Gly Ser Met Phe Glu 
    210                 215                 220                 


Gly Ile Leu Pro Glu Asp Leu Asn Ile Tyr Ala Leu Thr Ser Thr Asn 
225                 230                 235                 240 


Thr Thr Glu Ser Ser Trp Cys Tyr Tyr Cys Pro Ala Gln Glu Asn Pro 
                245                 250                 255     


Pro Pro Pro Glu Tyr Asn Val Cys Leu Gly Asp Leu Phe Ser Val Ala 
            260                 265                 270         


Trp Leu Glu Asp Ser Asp Val Gln Asn Ser Trp Tyr Glu Thr Leu Asn 
        275                 280                 285             


Gln Gln Tyr His His Val Asp Lys Arg Ile Ser His Ala Ser His Ala 
    290                 295                 300                 


Thr Gln Tyr Gly Asn Leu Lys Leu Gly Glu Glu Gly Leu Phe Val Tyr 
305                 310                 315                 320 


Met Gly Ser Asn Pro Ala Asn Asp Asn Tyr Thr Ser Leu Asp Gly Asn 
                325                 330                 335     


Ala Leu Thr Pro Ser Ser Ile Val Val Asn Gln Arg Asp Ala Asp Leu 
            340                 345                 350         


Leu His Leu Trp Glu Lys Phe Arg Lys Ala Pro Glu Gly Ser Ala Arg 
        355                 360                 365             


Lys Glu Val Ala Gln Thr Gln Ile Phe Lys Ala Met Ser His Arg Val 
    370                 375                 380                 


His Ile Asp Ser Ser Ile Lys Leu Ile Gly Lys Leu Leu Phe Gly Ile 
385                 390                 395                 400 


Glu Lys Cys Thr Glu Ile Leu Asn Ala Val Arg Pro Ala Gly Gln Pro 
                405                 410                 415     


Leu Val Asp Asp Trp Ala Cys Leu Arg Ser Leu Val Gly Thr Phe Glu 
            420                 425                 430         


Thr His Cys Gly Ser Leu Ser Glu Tyr Gly Met Arg His Thr Arg Thr 
        435                 440                 445             


Ile Ala Asn Ile Cys Asn Ala Gly Ile Ser Glu Glu Gln Met Ala Glu 
    450                 455                 460                 


Ala Ala Ser Gln Ala Cys Ala Ser Ile Pro 
465                 470                 


<210>  45
<211>  451
<212>  PRT
<213>  Oldenlandia affinis

<400>  45

Ala Arg Asp Gly Asp Tyr Leu His Leu Pro Ser Glu Val Ser Arg Phe 
1               5                   10                  15      


Phe Arg Pro Gln Glu Thr Asn Asp Asp His Gly Glu Asp Ser Val Gly 
            20                  25                  30          


Thr Arg Trp Ala Val Leu Ile Ala Gly Ser Lys Gly Tyr Ala Asn Tyr 
        35                  40                  45              


Arg His Gln Ala Gly Val Cys His Ala Tyr Gln Ile Leu Lys Arg Gly 
    50                  55                  60                  


Gly Leu Lys Asp Glu Asn Ile Val Val Phe Met Tyr Asp Asp Ile Ala 
65                  70                  75                  80  


Tyr Asn Glu Ser Asn Pro Arg Pro Gly Val Ile Ile Asn Ser Pro His 
                85                  90                  95      


Gly Ser Asp Val Tyr Ala Gly Val Pro Lys Asp Tyr Thr Gly Glu Glu 
            100                 105                 110         


Val Asn Ala Lys Asn Phe Leu Ala Ala Ile Leu Gly Asn Lys Ser Ala 
        115                 120                 125             


Ile Thr Gly Gly Ser Gly Lys Val Val Asp Ser Gly Pro Asn Asp His 
    130                 135                 140                 


Ile Phe Ile Tyr Tyr Thr Asp His Gly Ala Ala Gly Val Ile Gly Met 
145                 150                 155                 160 


Pro Ser Lys Pro Tyr Leu Tyr Ala Asp Glu Leu Asn Asp Ala Leu Lys 
                165                 170                 175     


Lys Lys His Ala Ser Gly Thr Tyr Lys Ser Leu Val Phe Tyr Leu Glu 
            180                 185                 190         


Ala Cys Glu Ser Gly Ser Met Phe Glu Gly Ile Leu Pro Glu Asp Leu 
        195                 200                 205             


Asn Ile Tyr Ala Leu Thr Ser Thr Asn Thr Thr Glu Ser Ser Trp Cys 
    210                 215                 220                 


Tyr Tyr Cys Pro Ala Gln Glu Asn Pro Pro Pro Pro Glu Tyr Asn Val 
225                 230                 235                 240 


Cys Leu Gly Asp Leu Phe Ser Val Ala Trp Leu Glu Asp Ser Asp Val 
                245                 250                 255     


Gln Asn Ser Trp Tyr Glu Thr Leu Asn Gln Gln Tyr His His Val Asp 
            260                 265                 270         


Lys Arg Ile Ser His Ala Ser His Ala Thr Gln Tyr Gly Asn Leu Lys 
        275                 280                 285             


Leu Gly Glu Glu Gly Leu Phe Val Tyr Met Gly Ser Asn Pro Ala Asn 
    290                 295                 300                 


Asp Asn Tyr Thr Ser Leu Asp Gly Asn Ala Leu Thr Pro Ser Ser Ile 
305                 310                 315                 320 


Val Val Asn Gln Arg Asp Ala Asp Leu Leu His Leu Trp Glu Lys Phe 
                325                 330                 335     


Arg Lys Ala Pro Glu Gly Ser Ala Arg Lys Glu Val Ala Gln Thr Gln 
            340                 345                 350         


Ile Phe Lys Ala Met Ser His Arg Val His Ile Asp Ser Ser Ile Lys 
        355                 360                 365             


Leu Ile Gly Lys Leu Leu Phe Gly Ile Glu Lys Cys Thr Glu Ile Leu 
    370                 375                 380                 


Asn Ala Val Arg Pro Ala Gly Gln Pro Leu Val Asp Asp Trp Ala Cys 
385                 390                 395                 400 


Leu Arg Ser Leu Val Gly Thr Phe Glu Thr His Cys Gly Ser Leu Ser 
                405                 410                 415     


Glu Tyr Gly Met Arg His Thr Arg Thr Ile Ala Asn Ile Cys Asn Ala 
            420                 425                 430         


Gly Ile Ser Glu Glu Gln Met Ala Glu Ala Ala Ser Gln Ala Cys Ala 
        435                 440                 445             


Ser Ile Pro 
    450     


<210>  46
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sortase Acceptor Site

<400>  46

Asn Pro Lys Thr Gly 
1               5   


<210>  47
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sortase Acceptor Site


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa is any amino acid

<400>  47

Xaa Pro Glu Thr Gly 
1               5   


<210>  48
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sortase Acceptor Site

<400>  48

Leu Gly Ala Thr Gly 
1               5   


<210>  49
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sortase Acceptor Site

<400>  49

Ile Pro Asn Thr Gly 
1               5   


<210>  50
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sortase Acceptor Site

<400>  50

Ile Pro Glu Thr Gly 
1               5   


<210>  51
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sortase Acceptor Site

<400>  51

Asn Ser Lys Thr Ala 
1               5   


<210>  52
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sortase Acceptor Site

<400>  52

Asn Pro Gln Thr Gly 
1               5   


<210>  53
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sortase Acceptor Site

<400>  53

Asn Ala Lys Thr Asn 
1               5   


<210>  54
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sortase Acceptor Site

<400>  54

Asn Pro Gln Ser Ser 
1               5   


<210>  55
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sortase Acceptor Site


<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Xaa is any amino acid

<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  Xaa is any amino acid

<400>  55

Leu Pro Xaa Thr Xaa 
1               5   


<210>  56
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sortase Acceptor Site


<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Xaa is Lys or Gln

<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  Xaa is Asn, Asp or Gly

<400>  56

Asn Pro Xaa Thr Xaa 
1               5   


<210>  57
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sortase Acceptor Site


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa is Leu, Ile, Val or Met

<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Xaa is any amino acid

<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  Xaa is Ser, Thr or Ala

<400>  57

Xaa Pro Xaa Xaa Gly 
1               5   


<210>  58
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Artificial Sequence


<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  Xaa is Ala, Cys or Ser

<400>  58

Leu Pro Glu Xaa Gly 
1               5   


<210>  59
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sortase Acceptor Site


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa is Ala, Pro or Ser

<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Xaa is any amino acid

<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  Xaa is Thr, Ser, Ala or Cys

<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  Xaa is n number of Gly

<400>  59

Leu Xaa Xaa Xaa Xaa 
1               5   


<210>  60
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sortase Acceptor Site


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa is Ala, Pro or Ser

<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Xaa is any amino acid

<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  Xaa is Thr, Ser, Ala or Cys

<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  Xaa is n number of Ala

<400>  60

Leu Xaa Xaa Xaa Xaa 
1               5   


<210>  61
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sortase Acceptor Site

<400>  61

Asn Pro Gln Thr Asn 
1               5   


<210>  62
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sortase Acceptor Site

<400>  62

Tyr Pro Arg Thr Gly 
1               5   


<210>  63
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sortase Acceptor Site

<400>  63

Ile Pro Gln Thr Gly 
1               5   


<210>  64
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sortase Acceptor Site

<400>  64

Val Pro Asp Thr Gly 
1               5   


<210>  65
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sortase Acceptor Site


<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Xaa is any amino acid

<400>  65

Leu Pro Xaa Thr Gly Ser 
1               5       


<210>  66
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sortase Acceptor Site


<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Xaa is any amino acid

<400>  66

Leu Pro Xaa Ser 
1               


<210>  67
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sortase Acceptor Site


<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Xaa is any amino acid

<400>  67

Leu Ala Xaa Thr 
1               


<210>  68
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sortase Acceptor Site


<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Xaa is any amino acid

<400>  68

Met Pro Xaa Thr 
1               


<210>  69
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sortase Acceptor Site


<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Xaa is any amino acid

<400>  69

Met Pro Xaa Thr Gly 
1               5   


<210>  70
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sortase Acceptor Site


<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Xaa is any amino acid

<400>  70

Leu Ala Xaa Ser 
1               


<210>  71
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sortase Acceptor Site


<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Xaa is any amino acid

<400>  71

Asn Pro Xaa Thr 
1               


<210>  72
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sortase Acceptor Site


<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Xaa is any amino acid

<400>  72

Asn Pro Xaa Thr Gly 
1               5   


<210>  73
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sortase Acceptor Site


<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Xaa is any amino acid

<400>  73

Asn Ala Xaa Thr 
1               


<210>  74
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sortase Acceptor Site


<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Xaa is any amino acid

<400>  74

Asn Ala Xaa Thr Gly 
1               5   


<210>  75
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sortase Acceptor Site


<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Xaa is any amino acid

<400>  75

Asn Ala Xaa Ser 
1               


<210>  76
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sortase Acceptor Site


<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Xaa is any amino acid

<400>  76

Asn Ala Xaa Ser Gly 
1               5   


<210>  77
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sortase Acceptor Site


<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Xaa is any amino acid

<400>  77

Leu Pro Xaa Pro 
1               


<210>  78
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sortase Acceptor Site


<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Xaa is any amino acid

<400>  78

Leu Pro Xaa Pro Gly 
1               5   


<210>  79
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Leucine-Based Motif


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa is any amino acid

<220>
<221>  MISC_FEATURE
<222>  (3)..(5)
<223>  Xaa is any amino acid

<400>  79

Xaa Asp Xaa Xaa Xaa Leu Leu 
1               5           


<210>  80
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Leucine-Based Motif


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa is any amino acid

<220>
<221>  MISC_FEATURE
<222>  (3)..(5)
<223>  Xaa is any amino acid

<400>  80

Xaa Glu Xaa Xaa Xaa Leu Leu 
1               5           


<210>  81
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Leucine-Based Motif


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa is any amino acid

<220>
<221>  MISC_FEATURE
<222>  (3)..(5)
<223>  Xaa is any amino acid

<400>  81

Xaa Glu Xaa Xaa Xaa Ile Leu 
1               5           


<210>  82
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Leucine-Based Motif


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa is any amino acid

<220>
<221>  MISC_FEATURE
<222>  (3)..(5)
<223>  Xaa is any amino acid

<400>  82

Xaa Glu Xaa Xaa Xaa Leu Met 
1               5           


<210>  83
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Tyrosine-Based Motif


<220>
<221>  MISC_FEATURE
<222>  (2)..(3)
<223>  Xaa is any amino acid

<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  Xaa is a hydrophobic amino acid

<400>  83

Tyr Xaa Xaa Xaa 
1               


<210>  84
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Enterokinase Cleavage Site

<400>  84

Asp Asp Asp Asp Lys 
1               5   


<210>  85
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Factor Xa Cleavage Site

<400>  85

Ile Glu Gly Arg 
1               


<210>  86
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Factor Xa Cleavage Site

<400>  86

Ile Asp Gly Arg 
1               


<210>  87
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  TEV Cleavage Site

<400>  87

Glu Asn Leu Tyr Phe Gln Gly 
1               5           


<210>  88
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Thrombin Cleavage Site

<400>  88

Leu Val Pro Arg Gly Ser 
1               5       


<210>  89
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PreScission Cleavage Site

<400>  89

Leu Glu Val Leu Phe Gln Gly Pro 
1               5               


<210>  90
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ADAM17 Cleavage Site

<400>  90

Pro Leu Ala Gln Ala Val Arg Ser Ser Ser 
1               5                   10  


<210>  91
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human Airway Trypsin-Like Protease (HAT) Cleavage Site

<400>  91

Ser Lys Gly Arg Ser Leu Ile Gly Arg Val 
1               5                   10  


<210>  92
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Elastase Cleavage Site

<400>  92

Met Glu Ala Val Thr Tyr 
1               5       


<210>  93
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Furin Cleavage Site


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa is any amino acid

<400>  93

Arg Xaa Arg Lys Arg 
1               5   


<210>  94
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Granzyme Cleavage Site

<400>  94

Ile Glu Pro Asp 
1               


<210>  95
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Caspase 2 Cleavage Site

<400>  95

Asp Val Ala Asp 
1               


<210>  96
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Caspase 3 Cleavage Site

<400>  96

Asp Met Gln Asp 
1               


<210>  97
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Caspase 4 Cleavage Site

<400>  97

Leu Glu Val Asp 
1               


<210>  98
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Caspase 7 Cleavage Site

<400>  98

Asp Glu Val Asp 
1               


<210>  99
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Caspase 9 Cleavage Site

<400>  99

Leu Glu His Asp 
1               


<210>  100
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Caspase 10 Cleavage Site

<400>  100

Ile Glu His Asp 
1               


<210>  101
<211>  23
<212>  PRT
<213>  Influenza virus

<400>  101

Gly Leu Phe Gly Ala Ile Ala Gly Phe Ile Glu Asn Gly Trp Glu Gly 
1               5                   10                  15      


Met Ile Asp Gly Trp Tyr Gly 
            20              


<210>  102
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sortase Acceptor Site


<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Xaa is any amino acid

<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  Xaa is n number of Ala

<400>  102

Leu Pro Xaa Thr Xaa 
1               5   


<210>  103
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sortase Acceptor Site


<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Xaa is any amino acid

<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  Xaa is n number of Gly

<400>  103

Leu Pro Xaa Ser Xaa 
1               5   


<210>  104
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sortase Acceptor Site


<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Xaa is any amino acid

<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  Xaa is n number of Gly

<400>  104

Leu Ala Xaa Thr Xaa 
1               5   


<210>  105
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sortase Acceptor Site


<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Xaa is any amino acid

<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  Xaa is n number of Gly

<400>  105

Leu Pro Xaa Thr Xaa 
1               5   


<210>  106
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sortase Acceptor Site


<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  Xaa is any amino acid

<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  Xaa is n number of Gly

<400>  106

Leu Pro Ala Xaa Xaa 
1               5   


<210>  107
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sortase Acceptor Site


<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Xaa is any amino acid

<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  Xaa is n number of Gly

<400>  107

Leu Pro Xaa Cys Xaa 
1               5   


<210>  108
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sortase Acceptor Site


<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Xaa is any amino acid

<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  Xaa is n number of Gly

<400>  108

Leu Ala Xaa Ser Xaa 
1               5   


<210>  109
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sortase Acceptor Site


<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Xaa is any amino acid

<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  Xaa is n number of Gly

<400>  109

Leu Pro Xaa Ala Xaa 
1               5   


<210>  110
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sortase Acceptor Site


<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Xaa is any amino acid

<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  Xaa is n number of Gly

<400>  110

Leu Ser Xaa Thr Xaa 
1               5   


<210>  111
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sortase Acceptor Site


<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Xaa is any amino acid

<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  Xaa is n number of Gly

<400>  111

Leu Arg Xaa Thr Xaa 
1               5   


<210>  112
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sortase Acceptor Site

<400>  112

Leu Pro Glu Ser Gly 
1               5   


<210>  113
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sortase Acceptor Site

<400>  113

Leu Ala Glu Thr Gly 
1               5   


<210>  114
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sortase Acceptor Site


<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Xaa is any amino acid

<400>  114

Leu Pro Xaa Thr Ala 
1               5   


<210>  115
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sortase Acceptor Site


<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Xaa is any amino acid

<400>  115

Leu Pro Xaa Ser Gly 
1               5   


<210>  116
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sortase Acceptor Site


<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Xaa is any amino acid

<400>  116

Leu Ala Xaa Thr Gly 
1               5   


<210>  117
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sortase Acceptor Site


<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Xaa is any amino acid

<400>  117

Leu Pro Xaa Thr Gly 
1               5   


<210>  118
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sortase Acceptor Site


<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  Xaa is any amino acid

<400>  118

Leu Pro Ala Xaa Gly 
1               5   


<210>  119
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sortase Acceptor Site


<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Xaa is any amino acid

<400>  119

Leu Pro Xaa Cys Gly 
1               5   


<210>  120
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sortase Acceptor Site


<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Xaa is any amino acid

<400>  120

Leu Ala Xaa Ser Gly 
1               5   


<210>  121
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sortase Acceptor Site


<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Xaa is any amino acid

<400>  121

Leu Pro Xaa Ala Gly 
1               5   


<210>  122
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sortase Acceptor Site


<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Xaa is any amino acid

<400>  122

Leu Ser Xaa Thr Gly 
1               5   


<210>  123
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sortase Acceptor Site


<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Xaa is any amino acid

<400>  123

Leu Arg Xaa Thr Gly 
1               5   


