                         SEQUENCE LISTING

<110>  THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK
 
<120>  MICROBIOME ENGINEERING THROUGH ENGINEERED MOBILE GENETIC ELEMENTS

<130>  15003-440PC0

<140>  PCT/US2020/013368
<141>  2020-01-13

<160>  41    

<170>  PatentIn version 3.5

<210>  1
<211>  163
<212>  DNA
<213>  Bacillus cellulosilyticus

<400>  1
gattgcatta ggttttagtt tcttgtataa tgcttaatgt tggtcactga caggctacga       60

tacggaaggt tgctcacgcc cggccccttt gccatggcta gtgtgtggaa atttccgagg      120

agcaagtcta tttccaaaaa tgggcgaaaa aggaggtaat aca                        163


<210>  2
<211>  163
<212>  DNA
<213>  Geobacillus sp.

<400>  2
gggagagctt caacggcgct tctacccatt tgcttggaaa ggatgaggag caggaagaaa       60

ttccgtcccc aatgcgacgg ccctttacat ccatgttgtt tgatagtata atggatacgg      120

attgaccaaa ttgttcattt agtcagtttg aaggatgagg agt                        163


<210>  3
<211>  165
<212>  DNA
<213>  Eggerthella lenta

<400>  3
gtgaaggata cggctgcggc acttcgacat cgccccatgt ggcggctttg aactgggctt       60

atgaaacgcg ttcacaacct tttttgacca tcggcgcgaa cgtggtatca tgcgttcagc      120

ttttgcccat acatactacg tgctcaatct aggaggattt catac                      165


<210>  4
<211>  110
<212>  DNA
<213>  Segmented filamentous bacteria

<400>  4
ctctagagta gtagattatt ttaggaattt agatgttttg tatgaaatag atgcttcgta       60

tggaattaat gaaattttta gtcaggtaaa aaaggtaata ggagaatatt                 110


<210>  5
<211>  110
<212>  DNA
<213>  Segmented filamentous bacteria

<400>  5
gttttaaatg atgaaaagaa atatttaggg aagattgttt cgacgcgaat tgttgatctg       60

gaaaatgatc accttatcgg acaagcttta aaataggagg atataaaaat                 110


<210>  6
<211>  110
<212>  DNA
<213>  Segmented filamentous bacteria

<400>  6
ataaggattc tttaaagaga gatatagtta tgtcaaagac tgtagaattt ttagtaaatc       60

aaaataaaaa aagaggtatt aaatagagtg tattttaaag gaggagactt                 110


<210>  7
<211>  110
<212>  DNA
<213>  Segmented filamentous bacteria

<400>  7
aaacaccaat aaaattagaa tatttaggag cgactttaaa aaagtttaat aagaattgtt       60

tatgagatat ttttattata tttaaactca atttaaagta gggagaatag                 110


<210>  8
<211>  126
<212>  DNA
<213>  Clostridium perfringens

<400>  8
gcaagtgttc aagaagttat taagtcggga gtgcagtcga agtgggcaag ttgaaaaatt       60

cacaaaaatg tggtataata tctttgttca ttagagcgat aaacttgaat ttgagaggga      120

acttag                                                                 126


<210>  9
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Himar

<400>  9
acaggttgga tgataagtcc ccggtct                                           27


<210>  10
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Tn5

<400>  10
ctgtctctta tacacatct                                                    19


<210>  11
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: linker

<400>  11

Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly 
1               5                   10                  15      


Gly Ser 
        


<210>  12
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: modified Himar1 inverted repeat (IR) sequence

<400>  12
tagaccggga cttatcatcc aacctgt                                           27


<210>  13
<211>  861
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Beta-lactamase (carbenicillin/ampicillin resistance)

<400>  13
atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct       60

gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca      120

cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc      180

gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc      240

cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg      300

gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta      360

tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc      420

ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt      480

gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg      540

cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct      600

tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc      660

tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct      720

cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac      780

acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc      840

tcactgatta agcattggta a                                                861


<210>  14
<211>  624
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CatP (chloramphenicol resistance), Derived from 
       pJIR750 plasmid from Clostridium perfringens

<400>  14
atggtatttg aaaaaattga taaaaatagt tggaacagaa aagagtattt tgaccactac       60

tttgcaagtg taccttgtac atacagcatg accgttaaag tggatatcac acaaataaag      120

gaaaagggaa tgaaactata tcctgcaatg ctttattata ttgcaatgat tgtaaaccgc      180

cattcagagt ttaggacggc aatcaatcaa gatggtgaat tggggatata tgatgagatg      240

ataccaagct atacaatatt tcacaatgat actgaaacat tttccagcct ttggactgag      300

tgtaagtctg actttaaatc atttttagca gattatgaaa gtgatacgca acggtatgga      360

aacaatcata gaatggaagg aaagccaaat gctccggaaa acatttttaa tgtatctatg      420

ataccgtggt caaccttcga tggctttaat ctgaatttgc agaaaggata tgattatttg      480

attcctattt ttactatggg gaaatattat aaagaagata acaaaattat acttcctttg      540

gcaattcaag ttcatcacgc agtatgtgac ggatttcaca tttgccgttt tgtaaacgaa      600

ttgcaggaat tgataaatag ttaa                                             624


<210>  15
<211>  1926
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Tet (tetracycline resistance)

<400>  15
atgaatatta taaatttagg aattcttgct cacattgatg caggaaaaac ttccgtaacc       60

gagaatctgc tgtttgccag tggagcaacg gaaaagtgcg gctgtgtgga taatggtgac      120

accataacgg actctatgga tatagagaaa cgtagaggaa ttactgttcg ggcttctacg      180

acatctatta tctggaatgg tgtgaaatgc aatatcattg acactccggg acacatggat      240

tttattgcgg aagtggagcg gacattcaaa atgcttgatg gagcagtcct catcttatcc      300

gcaaaggaag gcatacaagc gcagacaaag ttgctgttca atactttaca gaagctgcaa      360

atcccgacaa ttatatttat caataagatt gaccgagccg gtgtgaattt ggagcgtttg      420

tatctggata taaaagcaaa tctgtctcaa gatgtcctgt ttatgcaaaa tgttgtcgat      480

ggatcggttt atccggtttg ctcccaaaca tatataaagg aagaatacaa agaatttgta      540

tgcaaccatg acgacaatat attagaacga tatttggcgg atagcgaaat ttcaccggct      600

gattattgga atacgataat cgctcttgtg gcaaaagcca aagtctatcc ggtgctacat      660

ggatcagcaa tgttcaatat cggtatcaat gagttgttgg acgccatcac ttcttttata      720

cttcctccgg catcggtctc aaacagactt tcatcttatc tttataagat agagcatgac      780

cccaaaggac ataaaagaag ttttctaaaa ataattgacg gaagtctgag acttcgagac      840

gttgtaagaa tcaacgattc ggaaaaattc atcaagatta aaaatctaaa aactatcaat      900

cagggcagag agataaatgt tgatgaagtg ggcgccaatg atatcgcgat tgtagaggat      960

atggatgatt ttcgaatcgg aaattattta ggtgctgaac cttgtttgat tcaaggatta     1020

tcgcatcagc atcccgctct caaatcctcc gtccggccag acaggcccga agagagaagc     1080

aaggtgatat ccgctctgaa tacattgtgg attgaagacc cgtctttgtc cttttccata     1140

aactcatata gtgatgaatt ggaaatctcg ttatatggtt taacccaaaa ggaaatcata     1200

cagacattgc tggaagaacg attttccgta aaggtccatt ttgatgagat caagactata     1260

tacaaagaac gacctgtaaa aaaggtcaat aagattattc agatcgaagt gccgcccaac     1320

ccttattggg ccacaatagg gctgactctt gaacccttac cgttagggac agggttgcaa     1380

atcgaaagtg acatctccta tggttatctg aaccattctt ttcaaaatgc cgtttttgaa     1440

gggattcgta tgtcttgcca atccgggtta catggatggg aagtgactga tctgaaagta     1500

acttttactc aagccgagta ttatagcccg gtaagtacac ctgctgattt cagacagctg     1560

accccttatg tcttcaggct ggccttgcaa cagtcaggtg tggacattct cgaaccgatg     1620

ctctattttg agttgcagat accccaagcg gcaagttcca aagctattac agatttgcaa     1680

aaaatgatgt ctgagattga agacatcagt tgcaataatg agtggtgtca tattaaaggg     1740

aaagttccat taaatacaag taaagactat gcatcagaag taagttcata cactaagggc     1800

ttaggcattt ttatggttaa gccatgcggg tatcaaataa caaaaggcgg ttattctgat     1860

aatatccgca tgaacgaaaa agataaactt ttattcatgt tccaaaaatc aatgtcatca     1920

aaataa                                                                1926


<210>  16
<211>  1011
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Spec (spectinomycin resistance)

<400>  16
atgcgctcac gcaactggtc cagaaccttg accgaacgca gcggtggtaa cggcgcagtg       60

gcggttttca tggcttgtta tgactgtttt tttggggtac agtctatgcc tcgggcatcc      120

aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag caacgatgtt      180

acgcagcagg gcagtcgccc taaaacaaag ttaaacatca tgagggaagc ggtgatcgcc      240

gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg      300

ttgctggccg tacatttgta cggctccgca gtggatggcg gcctgaagcc acacagtgat      360

attgatttgc tggttacggt gaccgtaagg cttgatgaaa caacgcggcg agctttgatc      420

aacgaccttt tggaaacttc ggcttcccct ggagagagcg agattctccg cgctgtagaa      480

gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg      540

caatttggag aatggcagcg caatgacatt cttgcaggta tcttcgagcc agccacgatc      600

gacattgatc tggctatctt gctgacaaaa gcaagagaac atagcgttgc cttggtaggt      660

ccagcggcgg aggaactctt tgatccggtt cctgaacagg atctatttga ggcgctaaat      720

gaaaccttaa cgctatggaa ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg      780

cttacgttgt cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc      840

gctgccgact gggcaatgga gcgcctgccg gcccagtatc agcccgtcat acttgaagct      900

agacaggctt atcttggaca agaagaagat cgcttggcct cgcgcgcaga tcagttggaa      960

gaatttgtcc actacgtgaa aggcgagatc accaaggtag tcggcaaata a              1011


<210>  17
<211>  810
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Kan (kanamycin resistance)

<400>  17
atgagccata ttcaacggga aacgtcgagg ccgcgattaa attccaacat ggatgctgat       60

ttatatgggt ataaatgggc tcgcgataat gtcgggcaat caggtgcgac aatctatcgc      120

ttgtatggga agcccgatgc gccagagttg tttctgaaac atggcaaagg tagcgttgcc      180

aatgatgtta cagatgagat ggtcagacta aactggctga cggaatttat gcctcttccg      240

accatcaagc attttatccg tactcctgat gatgcatggt tactcaccac tgcgatcccc      300

ggaaaaacag cattccaggt attagaagaa tatcctgatt caggtgaaaa tattgttgat      360

gcgctggcag tgttcctgcg ccggttgcat tcgattcctg tttgtaattg tccttttaac      420

agcgatcgcg tatttcgtct cgctcaggcg caatcacgaa tgaataacgg tttggttgat      480

gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg      540

cataaacttt tgccattctc accggattca gtcgtcactc atggtgattt ctcacttgat      600

aaccttattt ttgacgaggg gaaattaata ggttgtattg atgttggacg agtcggaatc      660

gcagaccgat accaggatct tgccatccta tggaactgcc tcggtgagtt ttctccttca      720

ttacagaaac ggctttttca aaaatatggt attgataatc ctgatatgaa taaattgcag      780

tttcatttga tgctcgatga gtttttctaa                                       810


<210>  18
<211>  735
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: ermG (erythromycin resistance)

<400>  18
atgaacaaag taaatataaa agatagtcaa aattttatta cttcaaaata tcacatagaa       60

aaaataatga attgcataag tttagatgaa aaagataaca tctttgaaat aggtgcaggg      120

aaaggtcatt ttactgctgg attggtaaag agatgtaatt ttgtaacggc gatagaaatt      180

gattctaaat tatgtgaggt aactcgtaat aagctcttaa attatcctaa ctatcaaata      240

gtaaatgatg atatactgaa atttacattt cctagccaca atccatataa aatatttggc      300

agcatacctt acaacataag cacaaatata attcgaaaaa ttgtttttga aagttcagcc      360

acaataagtt atttaatagt ggaatatggt tttgctaaaa tgttattaga tacaaacaga      420

tcactagcat tgctgttaat ggcagaggta gatatttcta tattagcaaa aattcctagg      480

tattatttcc atccaaaacc taaagtggat agcacattaa ttgtattaaa aagaaagcca      540

gcaaaaatgg catttaaaga gagaaaaaaa tatgaaactt ttgtaatgaa atgggttaac      600

aaagagtacg aaaaactgtt tacaaaaaat caatttaata aagctttaaa acatgcgaga      660

atatatgata taaacaatat tagtttcgaa caatttgtat cgctatttaa tagttataaa      720

atatttaacg gctaa                                                       735


<210>  19
<211>  717
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: sfGFP

<400>  19
atgcgtaaag gcgaagagct gttcactggt ttcgtcacta ttctggtgga actggatggt       60

gatgtcaacg gtcataagtt ttccgtgcgt ggcgagggtg aaggtgacgc aactaatggt      120

aaactgacgc tgaagttcat ctgtactact ggtaaactgc cggtaccttg gccgactctg      180

gtaacgacgc tgacttatgg tgttcagtgc tttgctcgtt atccggacca catgaagcag      240

catgacttct tcaagtccgc catgccggaa ggctatgtgc aggaacgcac gatttccttt      300

aaggatgacg gcacgtacaa aacgcgtgcg gaagtgaaat ttgaaggcga taccctggta      360

aaccgcattg agctgaaagg cattgacttt aaagaagacg gcaatatcct gggccataag      420

ctggaataca attttaacag ccacaatgtt tacatcaccg ccgataaaca aaaaaatggc      480

attaaagcga attttaaaat tcgccacaac gtggaggatg gcagcgtgca gctggctgat      540

cactaccagc aaaacactcc aatcggtgat ggtcctgttc tgctgccaga caatcactat      600

ctgagcacgc aaagcgttct gtctaaagat ccgaacgaga aacgcgatca catggttctg      660

ctggagttcg taaccgcagc gggcatcacg catggtatgg atgaactgta caaataa         717


<210>  20
<211>  660
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Chlor (chloramphenicol resistance)

<400>  20
atggagaaaa aaatcactgg atataccacc gttgatatat cccaatggca tcgtaaagaa       60

cattttgagg catttcagtc agttgctcaa tgtacctata accagaccgt tcagctggat      120

attacggcct ttttaaagac cgtaaagaaa aataagcaca agttttatcc ggcctttatt      180

cacattcttg cccgcctgat gaatgctcat ccggaattac gtatggcaat gaaagacggt      240

gagctggtga tatgggatag tgttcaccct tgttacaccg ttttccatga gcaaactgaa      300

acgttttcat cgctctggag tgaataccac gacgatttcc ggcagtttct acacatatat      360

tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg gtttattgag      420

aatatgtttt tcgtctcagc caatccctgg gtgagtttca ccagttttga tttaaacgtg      480

gccaatatgg acaacttctt cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc      540

gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtttgtga tggcttccat      600

gtcggcagaa tgcttaatga attacaacag tactgcgatg agtggcaggg cggggcgtaa      660


<210>  21
<211>  312
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: R6K origin of replication, Requires additional pir 
       gene for replication

<400>  21
atccctggct tgttgtccac aaccgttaaa ccttaaaagc tttaaaagcc ttatatattc       60

ttttttttct tataaaactt aaaaccttag aggctattta agttgctgat ttatattaat      120

tttattgttc aaacatgaga gcttagtacg tgaaacatga gagcttagta cgttagccat      180

gagagcttag tacgttagcc atgagggttt agttcgttaa acatgagagc ttagtacgtt      240

aaacatgaga gcttagtacg tgaaacatga gagcttagta cgtactatca acaggttgaa      300

ctgctgatct tc                                                          312


<210>  22
<211>  797
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: p15A origin of replication

<400>  22
aacaacttat atcgtatggg gctgacttca ggtgctacat ttgaagagat aaattgcact       60

gaaatctaga aatattttat ctgattaata agatgatctt cttgagatcg ttttggtctg      120

cgcgtaatct cttgctctga aaacgaaaaa accgccttgc agggcggttt ttcgaaggtt      180

ctctgagcta ccaactcttt gaaccgaggt aactggcttg gaggagcgca gtcaccaaaa      240

cttgtccttt cagtttagcc ttaaccggcg catgacttca agactaactc ctctaaatca      300

attaccagtg gctgctgcca gtggtgcttt tgcatgtctt tccgggttgg actcaagacg      360

atagttaccg gataaggcgc agcggtcgga ctgaacgggg ggttcgtgca tacagtccag      420

cttggagcga actgcctacc cggaactgag tgtcaggcgt ggaatgagac aaacgcggcc      480

ataacagcgg aatgacaccg gtaaaccgaa aggcaggaac aggagagcgc acgagggagc      540

cgccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac cactgatttg      600

agcgtcagat ttcgtgatgc ttgtcagggg ggcggagcct atggaaaaac ggctttgccg      660

cggccctctc acttccctgt taagtatctt cctggcatct tccaggaaat ctccgccccg      720

ttcgtaagcc atttccgctc gccgcagtcg aacgaccgag cgtagcgagt cagtgagcga      780

ggaagcggaa tatatcc                                                     797


<210>  23
<211>  823
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: oriV, Requires trfA protein for replication

<400>  23
agcgggccgg gagggttcga gaaggggggg cacccccctt cggcgtgcgc ggtcacgcgc       60

cagggcgcag ccctggttaa aaacaaggtt tataaatatt ggtttaaaag caggttaaaa      120

gacaggttag cggtggccga aaaacgggcg gaaacccttg caaatgctgg attttctgcc      180

tgtggacagc ccctcaaatg tcaataggtg cgcccctcat ctgtcatcac tctgcccctc      240

aagtgtcaag gatcgcgccc ctcatctgtc agtagtcgcg cccctcaagt gtcaataccg      300

cagggcactt atccccaggc ttgtccacat catctgtggg aaactcgcgt aaaatcaggc      360

gttttcgccg atttgcgagg ctggccagct ccacgtcgcc ggccgaaatc gagcctgccc      420

ctcatctgtc aacgccgcgc cgggtgagtc ggcccctcaa gtgtcaacgt ccgcccctca      480

tctgtcagtg agggccaagt tttccgcgtg gtatccacaa cgccggcggc cgcggtgtct      540

cgcacacggc ttcgacggcg tttctggcgc gtttgcaggg ccatagacgg ccgccagccc      600

agcggcgagg gcaaccagcc cggtgagcgt cggaaaggcg ctggaagccc cgtagcgacg      660

cggagagggg cgagacaagc caagggcgca ggctcgatgc gcagcacgac atagccggtt      720

ctcgcaagga cgagaatttc cctgcggtgc ccctcaagtg tcaatgaaag tttccaacgc      780

gagccattcg cgagagcctt gagtccacgc tagatctatc tca                        823


<210>  24
<211>  1516
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: pBBR1 origin of replication, Includes coding sequence 
       of required replication protein

<400>  24
ctacgggctt gctctccggg cttcgccctg cgcggtcgct gcgctccctt gccagcccgt       60

ggatatgtgg acgatggccg cgagcggcca ccggctggct cgcttcgctc ggcccgtgga      120

caaccctgct ggacaagctg atggacaggc tgcgcctgcc cacgagcttg accacaggga      180

ttgcccaccg gctacccagc cttcgaccac atacccaccg gctccaactg cgcggcctgc      240

ggccttgccc catcaatttt tttaattttc tctggggaaa agcctccggc ctgcggcctg      300

cgcgcttcgc ttgccggttg gacaccaagt ggaaggcggg tcaaggctcg cgcagcgacc      360

gcgcagcggc ttggccttga cgcgcctgga acgacccaag cctatgcgag tgggggcagt      420

cgaaggcgaa gcccgcccgc ctgccccccg agcctcacgg cggcgagtgc gggggttcca      480

agggggcagc gccaccttgg gcaaggccga aggccgcgca gtcgatcaac aagccccgga      540

ggggccactt tttgccggag ggggagccgc gccgaaggcg tgggggaacc ccgcaggggt      600

gcccttcttt gggcaccaaa gaactagata tagggcgaaa tgcgaaagac ttaaaaatca      660

acaacttaaa aaaggggggt acgcaacagc tcattgcggc accccccgca atagctcatt      720

gcgtaggtta aagaaaatct gtaattgact gccactttta cgcaacgcat aattgttgtc      780

gcgctgccga aaagttgcag ctgattgcgc atggtgccgc aaccgtgcgg caccctaccg      840

catggagata agcatggcca cgcagtccag agaaatcggc attcaagcca agaacaagcc      900

cggtcactgg gtgcaaacgg aacgcaaagc gcatgaggcg tgggccgggc ttattgcgag      960

gaaacccacg gcggcaatgc tgctgcatca cctcgtggcg cagatgggcc accagaacgc     1020

cgtggtggtc agccagaaga cactttccaa gctcatcgga cgttctttgc ggacggtcca     1080

atacgcagtc aaggacttgg tggccgagcg ctggatctcc gtcgtgaagc tcaacggccc     1140

cggcaccgtg tcggcctacg tggtcaatga ccgcgtggcg tggggccagc cccgcgacca     1200

gttgcgcctg tcggtgttca gtgccgccgt ggtggttgat cacgacgacc aggacgaatc     1260

gctgttgggg catggcgacc tgcgccgcat cccgaccctg tatccgggcg agcagcaact     1320

accgaccggc cccggcgagg agccgcccag ccagcccggc attccgggca tggaaccaga     1380

cctgccagcc ttgaccgaaa cggaggaatg ggaacggcgc gggcagcagc gcctgccgat     1440

gcccgatgag ccgtgttttc tggacgatgg cgagccgttg gagccgccga cacgggtcac     1500

gctgccgcgc cggtag                                                     1516


<210>  25
<211>  6146
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: RSF1010 plasmid backbone, Includes genes for 
       mobilization proteins A, B, C and replication proteins A, B, C

<400>  25
gctcgaccag gcgtacgctt atgggtgcct ttccgcagct tggaacgcgg atggagaaga       60

ggagcaacgc gatctagcta tcgcggccgc gatcaagcag gtgcgacaga cgtcatacta      120

gatatcaagc gacttctcct atcccctggg aacacatcaa tctcaccgga gaatatcgct      180

ggccaaagcc ttagcgtagg attccgcccc ttcccgcaaa cgaccccaaa caggaaacgc      240

agctgaaacg ggaagctcaa cacccactga cgcatgggtt gttcaggcag tacttcatca      300

accagcaagg cggcactttc ggccatccgc cgcgccccac agctcgggca gaaaccgcga      360

cgcttacagc tgaaagcgac caggtgctcg gcgtggcaag actcgcagcg aacccgtaga      420

aagccatgct ccagccgccc gcattggaga aattcttcaa attcccgttg cacatagccc      480

ggcaattcct ttccctgctc tgccataagc gcagcgaatg ccgggtaata ctcgtcaacg      540

atctgataga gaagggtttg ctcgggtcgg tggctctggt aacgaccagt atcccgatcc      600

cggctggccg tcctggccgc cacatgaggc atgttccgcg tccttgcaat actgtgttta      660

catacagtct atcgcttagc ggaaagttct tttaccctca gccgaaatgc ctgccgttgc      720

tagacattgc cagccagtgc ccgtcactcc cgtactaact gtcacgaacc cctgcaataa      780

ctgtcacgcc cccctgcaat aactgtcacg aacccctgca ataactgtca cgcccccaaa      840

cctgcaaacc cagcaggggc gggggctggc ggggtgttgg aaaaatccat ccatgattat      900

ctaagaataa tccactaggc gcggttatca gcgcccttgt ggggcgctgc tgcccttgcc      960

caatatgccc ggccagaggc cggatagctg gtctattcgc tgcgctaggc tacacaccgc     1020

cccaccgctg cgcggcaggg ggaaaggcgg gcaaagcccg ctaaacccca caccaaaccc     1080

cgcagaaata cgctggagcg cttttagccg ctttagcggc ctttccccct acccgaaggg     1140

tgggggcgcg tgtgcagccc cgcagggcct gtctcggtcg atcattcagc ccggctcatc     1200

cttctggcgt ggcggcagac cgaacaaggc gcggtcgtgg tcgcgttcaa ggtacgcatc     1260

cattgccgcc atgagccgat cctccggcca ctcgctgctg ttcaccttgg ccaaaatcat     1320

ggcccccacc agcaccttgc gccttgtttc gttcttgcgc tcttgctgct gttcccttgc     1380

ccgcacccgc tgaatttcgg cattgattcg cgctcgttgt tcttcgagct tggccagccg     1440

atccgccgcc ttgttgctcc ccttaaccat cttgacaccc cattgttaat gtgctgtctc     1500

gtaggctatc atggaggcac agcggcggca atcccgaccc tactttgtag gggagggcgc     1560

acttaccggt ttctcttcga gaaactggcc taacggccac ccttcgggcg gtgcgctctc     1620

cgagggccat tgcatggagc cgaaaagcaa aagcaacagc gaggcagcat ggcgatttat     1680

caccttacgg cgaaaaccgg cagcaggtcg ggcggccaat cggccagggc caaggccgac     1740

tacatccagc gcgaaggcaa gtatgcccgc gacatggatg aagtcttgca cgccgaatcc     1800

gggcacatgc cggagttcgt cgagcggccc gccgactact gggatgctgc cgacctgtat     1860

gaacgcgcca atgggcggct gttcaaggag gtcgaatttg ccctgccggt cgagctgacc     1920

ctcgaccagc agaaggcgct ggcgtccgag ttcgcccagc acctgaccgg tgccgagcgc     1980

ctgccgtata cgctggccat ccatgccggt ggcggcgaga acccgcactg ccacctgatg     2040

atctccgagc ggatcaatga cggcatcgag cggcccgccg ctcagtggtt caagcggtac     2100

aacggcaaga ccccggagaa gggcggggca cagaagaccg aagcgctcaa gcccaaggca     2160

tggcttgagc agacccgcga ggcatgggcc gaccatgcca accgggcatt agagcgggct     2220

ggccacgacg cccgcattga ccacagaaca cttgaggcgc agggcatcga gcgcctgccc     2280

ggtgttcacc tggggccgaa cgtggtggag atggaaggcc ggggcatccg caccgaccgg     2340

gcagacgtgg ccctgaacat cgacaccgcc aacgcccaga tcatcgactt acaggaatac     2400

cgggaggcaa tagaccatga acgcaatcga cagagtgaag aaatccagag gcatcaacga     2460

gttagcggag cagatcgaac cgctggccca gagcatggcg acactggccg acgaagcccg     2520

gcaggtcatg agccagaccc agcaggccag cgaggcgcag gcggcggagt ggctgaaagc     2580

ccagcgccag acaggggcgg catgggtgga gctggccaaa gagttgcggg aggtagccgc     2640

cgaggtgagc agcgccgcgc agagcgcccg gagcgcgtcg cgggggtggc actggaagct     2700

atggctaacc gtgatgctgg cttccatgat gcctacggtg gtgctgctga tcgcatcgtt     2760

gctcttgctc gacctgacgc cactgacaac cgaggacggc tcgatctggc tgcgcttggt     2820

ggcccgatga agaacgacag gactttgcag gccataggcc gacagctcaa ggccatgggc     2880

tgtgagcgct tcgatatcgg cgtcagggac gccaccaccg gccagatgat gaaccgggaa     2940

tggtcagccg ccgaagtgct ccagaacacg ccatggctca agcggatgaa tgcccagggc     3000

aatgacgtgt atatcaggcc cgccgagcag gagcggcatg gtctggtgct ggtggacgac     3060

ctcagcgagt ttgacctgga tgacatgaaa gccgagggcc gggagcctgc cctggtagtg     3120

gaaaccagcc cgaagaacta tcaggcatgg gtcaaggtgg ccgacgccgc aggcggtgaa     3180

cttcgggggc agattgcccg gacgctggcc agcgagtacg acgccgaccc ggccagcgcc     3240

gacagccgcc actatggccg cttggcgggc ttcaccaacc gcaaggacaa gcacaccacc     3300

cgcgccggtt atcagccgtg ggtgctgctg cgtgaatcca agggcaagac cgccaccgct     3360

ggcccggcgc tggtgcagca ggctggccag cagatcgagc aggcccagcg gcagcaggag     3420

aaggcccgca ggctggccag cctcgaactg cccgagcggc agcttagccg ccaccggcgc     3480

acggcgctgg acgagtaccg cagcgagatg gccgggctgg tcaagcgctt cggtgatgac     3540

ctcagcaagt gcgactttat cgccgcgcag aagctggcca gccggggccg cagtgccgag     3600

gaaatcggca aggccatggc cgaggccagc ccagcgctgg cagagcgcaa gcccggccac     3660

gaagcggatt acatcgagcg caccgtcagc aaggtcatgg gtctgcccag cgtccagctt     3720

gcgcgggccg agctggcacg ggcaccggca ccccgccagc gaggcatgga caggggcggg     3780

ccagatttca gcatgtagtg cttgcgttgg tactcacgcc tgttatacta tgagtactca     3840

cgcacagaag ggggttttat ggaatacgaa aaaagcgctt cagggtcggt ctacctgatc     3900

aaaagtgaca agggctattg gttgcccggt ggctttggtt atacgtcaaa caaggccgag     3960

gctggccgct tttcagtcgc tgatatggcc agccttaacc ttgacggctg caccttgtcc     4020

ttgttccgcg aagacaagcc tttcggcccc ggcaagtttc tcggtgactg atatgaaaga     4080

ccaaaaggac aagcagaccg gcgacctgct ggccagccct gacgctgtac gccaagcgcg     4140

atatgccgag cgcatgaagg ccaaagggat gcgtcagcgc aagttctggc tgaccgacga     4200

cgaatacgag gcgctgcgcg agtgcctgga agaactcaga gcggcgcagg gcgggggtag     4260

tgaccccgcc agcgcctaac caccaactgc ctgcaaagga ggcaatcaat ggctacccat     4320

aagcctatca atattctgga ggcgttcgca gcagcgccgc caccgctgga ctacgttttg     4380

cccaacatgg tggccggtac ggtcggggcg ctggtgtcgc ccggtggtgc cggtaaatcc     4440

atgctggccc tgcaactggc cgcacagatt gcaggcgggc cggatctgct ggaggtgggc     4500

gaactgccca ccggcccggt gatctacctg cccgccgaag acccgcccac cgccattcat     4560

caccgcctgc acgcccttgg ggcgcacctc agcgccgagg aacggcaagc cgtggctgac     4620

ggcctgctga tccagccgct gatcggcagc ctgcccaaca tcatggcccc ggagtggttc     4680

gacggcctca agcgcgccgc cgagggccgc cgcctgatgg tgctggacac gctgcgccgg     4740

ttccacatcg aggaagaaaa cgccagcggc cccatggccc aggtcatcgg tcgcatggag     4800

gccatcgccg ccgataccgg gtgctctatc gtgttcctgc accatgccag caagggcgcg     4860

gccatgatgg gcgcaggcga ccagcagcag gccagccggg gcagctcggt actggtcgat     4920

aacatccgct ggcagtccta cctgtcgagc atgaccagcg ccgaggccga ggaatggggt     4980

gtggacgacg accagcgccg gttcttcgtc cgcttcggtg tgagcaaggc caactatggc     5040

gcaccgttcg ctgatcggtg gttcaggcgg catgacggcg gggtgctcaa gcccgccgtg     5100

ctggagaggc agcgcaagag caagggggtg ccccgtggtg aagcctaaga acaagcacag     5160

cctcagccac gtccggcacg acccggcgca ctgtctggcc cccggcctgt tccgtgccct     5220

caagcggggc gagcgcaagc gcagcaagct ggacgtgacg tatgactacg gcgacggcaa     5280

gcggatcgag ttcagcggcc cggagccgct gggcgctgat gatctgcgca tcctgcaagg     5340

gctggtggcc atggctgggc ctaatggcct agtgcttggc ccggaaccca agaccgaagg     5400

cggacggcag ctccggctgt tcctggaacc caagtgggag gccgtcaccg ctgaatgcca     5460

tgtggtcaaa ggtagctatc gggcgctggc aaaggaaatc ggggcagagg tcgatagtgg     5520

tggggcgctc aagcacatac aggactgcat cgagcgcctt tggaaggtat ccatcatcgc     5580

ccagaatggc cgcaagcggc aggggtttcg gctgctgtcg gagtacgcca gcgacgaggc     5640

ggacgggcgc ctgtacgtgg ccctgaaccc cttgatcgcg caggccgtca tgggtggcgg     5700

ccagcatgtg cgcatcagca tggacgaggt gcgggcgctg gacagcgaaa ccgcccgcct     5760

gctgcaccag cggctgtgtg gctggatcga ccccggcaaa accggcaagg cttccataga     5820

taccttgtgc ggctatgtct ggccgtcaga ggccagtggt tcgaccatgc gcaagcgccg     5880

ccagcgggtg cgcgaggcgt tgccggagct ggtcgcgctg ggctggacgg taaccgagtt     5940

cgcggcgggc aagtacgaca tcacccggcc caaggcggca ggctgacccc ccccactcta     6000

ttgtaaacaa gacattttta tcttttatat tcaatggctt attttcctgc taattggtaa     6060

taccatgaaa aataccatgc tcagaaaagg cttaacaata ttttgaaaaa ttgcctactg     6120

agcgctgccg cacagctcca taggcc                                          6146


<210>  26
<211>  1540
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: RCR

<400>  26
tccgccgccc tagacctagt gtcattttat ttcccccgtt tcagcatcaa gaacctttgc       60

ataacttgct ctatatccac actgataatt gccctcaaac cataatctaa aggcgctaga      120

gtttgttgaa acaatatctt ttacatcatt cgtatttaaa attccaaact ccgctcccct      180

aaggcgaata aaagccatta aatcttttgt atttaccaaa ttatagtcat ccactatatc      240

taagagtaaa ttcttcaatt ctcttttttg gctttcatca agtgttatat agcggtcaat      300

atcaaaatca ttaatgttca aaatatcttt tttgtcgtat atatgtttat tcttagcaat      360

agcgtccttt gattcatgag tcaaatattc atatgaacct ttgatataat caagtatctc      420

aacatgagca actgaactat tccccaattt tcgcttaatc ttgttcctaa cgctttctat      480

tgttacagga tttcgtgcaa tatatataac gtgatagtgt ggttttttat agtgctttcc      540

atttcgtata acatcactac tattccatgt atctttatct tttttttcgt ccatatcgtg      600

taaaggactg acagccatag atacgcccaa actctctaat ttttccttcc aatcattagg      660

aattgagtca ggatataata aaaatccaaa atttctagct ttagtatttt taatagccat      720

gatataatta ccttatcaaa aacaagtagc gaaaactcgt atccttctaa aaacgcgagc      780

tttcgcttat tttttttgtt ctgattcctt tcttgcatat tcttctatag ctaacgccgc      840

aaccgcagat tttgaaaaac ctttttgttt cgccatatct gttaattttt tatcttgctc      900

ttttgtcaga gaaatcataa ctcttttttt cgattctgaa atcaccattt aaaaaactcc      960

aatcaaataa ttttataaag ttagtgtatc actttgtaat cataaaaaca acaataaagc     1020

tacttaaata tagatttata aaaaacgttg gcgaaaacgt tggcgattcg ttggcgattg     1080

aaaaacccct taaacccttg agccagttgg gatagagcgt ttttggcaca aaaattggca     1140

ctcggcactt aatggggggt cgtagtacgg aagcaaaatt cgcttccttt ccccccattt     1200

ttttccaaat tccaaatttt tttcaaaaat tttccagcgc taccgctcgg caaaattgca     1260

agcaattttt aaaatcaaac ccatgaggga atttcattcc ctcatactcc cttgagcctc     1320

ctccaaccga aatagaaggg cgctgcgctt attatttcat tcagtcatcg gctttcataa     1380

tctaacagac aacatcttcg ctgcaaagcc acgctacgct caagggcttt tacgctacga     1440

taacgcctgt tttaacgatt atgccgataa ctaaacgaaa taaacgctaa aacgtctcag     1500

aaacgatttt gagacgtttt aataaaaaat cgcctagtgc                           1540


<210>  27
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: 16S forward

<400>  27
agagtttgat catggctcag                                                   20


<210>  28
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: 16S reverse

<400>  28
cggttacctt gttacgactt                                                   20


<210>  29
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: GFP validation primer forward

<400>  29
atgcgtaaag gcgaagagc                                                    19


<210>  30
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: GFP validation primer reverse

<400>  30
ttatttgtac agttcatcca taccatg                                           27


<210>  31
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Beta-lactamase validation primer forward

<400>  31
atgagtattc aacatttccg tgtc                                              24


<210>  32
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Beta-lactamase validation primer reverse

<400>  32
ttaccaatgc ttaatcagtg aggc                                              24


<210>  33
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: pGT-B backbone validation primer forward

<400>  33
ctgcgcaacc caagtgctac                                                   20


<210>  34
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: pGT-B backbone validation primer reverse

<400>  34
cagtccagag aaatcggcat tca                                               23


<210>  35
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: pGT-Ah backbone validation primer forward

<400>  35
atggaaaaaa aggaatttcg tgttttg                                           27


<210>  36
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: pGT-Ah backbone validation primer reverse

<400>  36
ttattcaaca tagttccctt caagagc                                           27


<210>  37
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CarbR internal forward primer

<400>  37
ccgaagaacg ttttccaatg atgag                                             25


<210>  38
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: GFP internal reverse primer

<400>  38
tgattgtctg gcagcagaac                                                   20


<210>  39
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: catP (chlor resistance) validation primer forward

<400>  39
gcaagtgttc aagaagttat taagtc                                            26


<210>  40
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: catP (chlor resistance) validation primer reverse

<400>  40
ttaactattt atcaattcct gcaattcg                                          28


<210>  41
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: tetQ (tet resistance) internal forward primer

<400>  41
tggaagaacg attttccgta aaggt                                             25


