                         SEQUENCE LISTING

<110>  Research Institute at Nationwide Children's Hospital
 
<120>  DUX4 RNA SILENCING USING RNA TARGETING CRISPR-CAS13B

<130>  28335/53307A

<150>  US 62/786,670
<151>  2018-12-31

<160>  66    

<170>  PatentIn version 3.5

<210>  1
<211>  1275
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleoide


<220>
<221>  misc_feature
<223>  DUX4 NT

<400>  1
atggccctcc cgacaccctc ggacagcacc ctccccgcgg aagcccgggg acgaggacgg       60

cgacggagac tcgtttggac cccgagccaa agcgaggccc tgcgagcctg ctttgagcgg      120

aacccgtacc cgggcatcgc caccagagaa cggctggccc aggccatcgg cattccggag      180

cccagggtcc agatttggtt tcagaatgag aggtcacgcc agctgaggca gcaccggcgg      240

gaatctcggc cctggcccgg gagacgcggc ccgccagaag gccggcgaaa gcggaccgcc      300

gtcaccggat cccagaccgc cctgctcctc cgagcctttg agaaggatcg ctttccaggc      360

atcgccgccc gggaggagct ggccagagag acgggcctcc cggagtccag gattcagatc      420

tggtttcaga atcgaagggc caggcacccg ggacagggtg gcagggcgcc cgcgcaggca      480

ggcggcctgt gcagcgcggc ccccggcggg ggtcaccctg ctccctcgtg ggtcgccttc      540

gcccacaccg gcgcgtgggg aacggggctt cccgcacccc acgtgccctg cgcgcctggg      600

gctctcccac agggggcttt cgtgagccag gcagcgaggg ccgcccccgc gctgcagccc      660

agccaggccg cgccggcaga ggggatctcc caacctgccc cggcgcgcgg ggatttcgcc      720

tacgccgccc cggctcctcc ggacggggcg ctctcccacc ctcaggctcc tcggtggcct      780

ccgcacccgg gcaaaagccg ggaggaccgg gacccgcagc gcgacggcct gccgggcccc      840

tgcgcggtgg cacagcctgg gcccgctcaa gcggggccgc agggccaagg ggtgcttgcg      900

ccacccacgt cccaggggag tccgtggtgg ggctggggcc ggggtcccca ggtcgccggg      960

gcggcgtggg aaccccaagc cggggcagct ccacctcccc agcccgcgcc cccggacgcc     1020

tccgcctccg cgcggcaggg gcagatgcaa ggcatcccgg cgccctccca ggcgctccag     1080

gagccggcgc cctggtctgc actcccctgc ggcctgctgc tggatgagct cctggcgagc     1140

ccggagtttc tgcagcaggc gcaacctctc ctagaaacgg aggccccggg ggagctggag     1200

gcctcggaag aggccgcctc gctggaagca cccctcagcg aggaagaata ccgggctctg     1260

ctggaggagc tttag                                                      1275


<210>  2
<211>  424
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<223>  DUX4 AA

<400>  2

Met Ala Leu Pro Thr Pro Ser Asp Ser Thr Leu Pro Ala Glu Ala Arg 
1               5                   10                  15      


Gly Arg Gly Arg Arg Arg Arg Leu Val Trp Thr Pro Ser Gln Ser Glu 
            20                  25                  30          


Ala Leu Arg Ala Cys Phe Glu Arg Asn Pro Tyr Pro Gly Ile Ala Thr 
        35                  40                  45              


Arg Glu Arg Leu Ala Gln Ala Ile Gly Ile Pro Glu Pro Arg Val Gln 
    50                  55                  60                  


Ile Trp Phe Gln Asn Glu Arg Ser Arg Gln Leu Arg Gln His Arg Arg 
65                  70                  75                  80  


Glu Ser Arg Pro Trp Pro Gly Arg Arg Gly Pro Pro Glu Gly Arg Arg 
                85                  90                  95      


Lys Arg Thr Ala Val Thr Gly Ser Gln Thr Ala Leu Leu Leu Arg Ala 
            100                 105                 110         


Phe Glu Lys Asp Arg Phe Pro Gly Ile Ala Ala Arg Glu Glu Leu Ala 
        115                 120                 125             


Arg Glu Thr Gly Leu Pro Glu Ser Arg Ile Gln Ile Trp Phe Gln Asn 
    130                 135                 140                 


Arg Arg Ala Arg His Pro Gly Gln Gly Gly Arg Ala Pro Ala Gln Ala 
145                 150                 155                 160 


Gly Gly Leu Cys Ser Ala Ala Pro Gly Gly Gly His Pro Ala Pro Ser 
                165                 170                 175     


Trp Val Ala Phe Ala His Thr Gly Ala Trp Gly Thr Gly Leu Pro Ala 
            180                 185                 190         


Pro His Val Pro Cys Ala Pro Gly Ala Leu Pro Gln Gly Ala Phe Val 
        195                 200                 205             


Ser Gln Ala Ala Arg Ala Ala Pro Ala Leu Gln Pro Ser Gln Ala Ala 
    210                 215                 220                 


Pro Ala Glu Gly Ile Ser Gln Pro Ala Pro Ala Arg Gly Asp Phe Ala 
225                 230                 235                 240 


Tyr Ala Ala Pro Ala Pro Pro Asp Gly Ala Leu Ser His Pro Gln Ala 
                245                 250                 255     


Pro Arg Trp Pro Pro His Pro Gly Lys Ser Arg Glu Asp Arg Asp Pro 
            260                 265                 270         


Gln Arg Asp Gly Leu Pro Gly Pro Cys Ala Val Ala Gln Pro Gly Pro 
        275                 280                 285             


Ala Gln Ala Gly Pro Gln Gly Gln Gly Val Leu Ala Pro Pro Thr Ser 
    290                 295                 300                 


Gln Gly Ser Pro Trp Trp Gly Trp Gly Arg Gly Pro Gln Val Ala Gly 
305                 310                 315                 320 


Ala Ala Trp Glu Pro Gln Ala Gly Ala Ala Pro Pro Pro Gln Pro Ala 
                325                 330                 335     


Pro Pro Asp Ala Ser Ala Ser Ala Arg Gln Gly Gln Met Gln Gly Ile 
            340                 345                 350         


Pro Ala Pro Ser Gln Ala Leu Gln Glu Pro Ala Pro Trp Ser Ala Leu 
        355                 360                 365             


Pro Cys Gly Leu Leu Leu Asp Glu Leu Leu Ala Ser Pro Glu Phe Leu 
    370                 375                 380                 


Gln Gln Ala Gln Pro Leu Leu Glu Thr Glu Ala Pro Gly Glu Leu Glu 
385                 390                 395                 400 


Ala Ser Glu Glu Ala Ala Ser Leu Glu Ala Pro Leu Ser Glu Glu Glu 
                405                 410                 415     


Tyr Arg Ala Leu Leu Glu Glu Leu 
            420                 


<210>  3
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleoide


<220>
<221>  misc_feature
<223>  gRNA1 sequence

<400>  3
ggcccttcga ttctgaaacc agatctgaat                                        30


<210>  4
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleoide


<220>
<221>  misc_feature
<223>  gRNA2 sequence

<400>  4
gattctgaaa ccagatctga atcctggact                                        30


<210>  5
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleoide


<220>
<221>  misc_feature
<223>  gRNA3 sequence

<400>  5
gactccggga ggcccgtctc tctggccagc t                                      31


<210>  6
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleoide


<220>
<221>  misc_feature
<223>  gRNA4 sequence

<400>  6
gaggagcctg agggtgggag agcgccccgt                                        30


<210>  7
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleoide


<220>
<221>  misc_feature
<223>  gRNA5 sequence

<400>  7
gcttttgccc gggtgcggag gccaccgagg ag                                     32


<210>  8
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleoide


<220>
<221>  misc_feature
<223>  gRNA6 sequence

<400>  8
tctaggagag gttgcgcctg ctgcagaaac t                                      31


<210>  9
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleoide


<220>
<221>  misc_feature
<223>  gRNA7 sequence

<400>  9
gtttctagga gaggttgcgc ctgctgcaga aact                                   34


<210>  10
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleoide


<220>
<221>  misc_feature
<223>  gRNA8 sequence

<400>  10
tggcagttct ccgcggtgtg gagtctctca                                        30


<210>  11
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleoide


<220>
<221>  misc_feature
<223>  gRNA9 sequence

<400>  11
gttctccgcg gtgtggagtc tctcaccggg                                        30


<210>  12
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleoide


<220>
<221>  misc_feature
<223>  gRNA10 sequence

<400>  12
gaacaagggc acagagaggc cagcgagctc                                        30


<210>  13
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleoide


<220>
<221>  misc_feature
<223>  gRNA11 sequence

<400>  13
gacagcgtcg gaaggtgggg ggagacattc ag                                     32


<210>  14
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleoide


<220>
<221>  misc_feature
<223>  gRNA1 DUX4 targeting sequence

<400>  14
attcagatct ggtttcagaa tcgaagggcc                                        30


<210>  15
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleoide


<220>
<221>  misc_feature
<223>  gRNA2 DUX4 targeting sequence

<400>  15
agtccaggat tcagatctgg tttcagaatc                                        30


<210>  16
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleoide


<220>
<221>  misc_feature
<223>  gRNA3 DUX4 targeting sequence

<400>  16
agctggccag agagacgggc ctcccggagt c                                      31


<210>  17
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleoide


<220>
<221>  misc_feature
<223>  gRNA4 DUX4 targeting sequence

<400>  17
acggggcgct ctcccaccct caggctcctc                                        30


<210>  18
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleoide


<220>
<221>  misc_feature
<223>  gRNA5 DUX4 targeting sequence

<400>  18
ctcctcggtg gcctccgcac ccgggcaaaa gc                                     32


<210>  19
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleoide


<220>
<221>  misc_feature
<223>  gRNA6 DUX4 targeting sequence

<400>  19
agtttctgca gcaggcgcaa cctctcctag a                                      31


<210>  20
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleoide


<220>
<221>  misc_feature
<223>  gRNA7 DUX4 targeting sequence

<400>  20
agtttctgca gcaggcgcaa cctctcctag aaac                                   34


<210>  21
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleoide


<220>
<221>  misc_feature
<223>  gRNA8 DUX4 targeting sequence

<400>  21
tgagagactc cacaccgcgg agaactgcca                                        30


<210>  22
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleoide


<220>
<221>  misc_feature
<223>  gRNA9 DUX4 targeting sequence

<400>  22
cccggtgaga gactccacac cgcggagaac                                        30


<210>  23
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleoide


<220>
<221>  misc_feature
<223>  gRNA10 DUX4 targeting sequence

<400>  23
gagctcgctg gcctctctgt gcccttgttc                                        30


<210>  24
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleoide


<220>
<221>  misc_feature
<223>  gRNA11 DUX4 targeting sequence

<400>  24
ctgaatgtct ccccccacct tccgacgctg tc                                     32


<210>  25
<211>  66
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleoide


<220>
<221>  misc_feature
<223>  gRNA1 + Cas13b direct repeat

<400>  25
ggcccttcga ttctgaaacc agatctgaat gttgtggaag gtccagtttt gaggggctat       60

tacaac                                                                  66


<210>  26
<211>  66
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleoide


<220>
<221>  misc_feature
<223>  gRNA2 + Cas13b direct repeat

<400>  26
gattctgaaa ccagatctga atcctggact gttgtggaag gtccagtttt gaggggctat       60

tacaac                                                                  66


<210>  27
<211>  67
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleoide


<220>
<221>  misc_feature
<223>  gRNA3 + Cas13b direct repeat

<400>  27
gactccggga ggcccgtctc tctggccagc tgttgtggaa ggtccagttt tgaggggcta       60

ttacaac                                                                 67


<210>  28
<211>  66
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleoide


<220>
<221>  misc_feature
<223>  gRNA4 + Cas13b direct repeat

<400>  28
gaggagcctg agggtgggag agcgccccgt gttgtggaag gtccagtttt gaggggctat       60

tacaac                                                                  66


<210>  29
<211>  68
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleoide


<220>
<221>  misc_feature
<223>  gRNA5 + Cas13b direct repeat

<400>  29
gcttttgccc gggtgcggag gccaccgagg aggttgtgga aggtccagtt ttgaggggct       60

attacaac                                                                68


<210>  30
<211>  67
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleoide


<220>
<221>  misc_feature
<223>  gRNA6 + Cas13b direct repeat

<400>  30
tctaggagag gttgcgcctg ctgcagaaac tgttgtggaa ggtccagttt tgaggggcta       60

ttacaac                                                                 67


<210>  31
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleoide


<220>
<221>  misc_feature
<223>  gRNA7 + Cas13b direct repeat

<400>  31
gtttctagga gaggttgcgc ctgctgcaga aactgttgtg gaaggtccag ttttgagggg       60

ctattacaac                                                              70


<210>  32
<211>  66
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleoide


<220>
<221>  misc_feature
<223>  gRNA8 + Cas13b direct repeat

<400>  32
tggcagttct ccgcggtgtg gagtctctca gttgtggaag gtccagtttt gaggggctat       60

tacaac                                                                  66


<210>  33
<211>  66
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleoide


<220>
<221>  misc_feature
<223>  gRNA9 + Cas13b direct repeat

<400>  33
gttctccgcg gtgtggagtc tctcaccggg gttgtggaag gtccagtttt gaggggctat       60

tacaac                                                                  66


<210>  34
<211>  66
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleoide


<220>
<221>  misc_feature
<223>  gRNA10 + Cas13b direct repeat

<400>  34
gaacaagggc acagagaggc cagcgagctc gttgtggaag gtccagtttt gaggggctat       60

tacaac                                                                  66


<210>  35
<211>  68
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleoide


<220>
<221>  misc_feature
<223>  gRNA11 + Cas13b direct repeat

<400>  35
gacagcgtcg gaaggtgggg ggagacattc aggttgtgga aggtccagtt ttgaggggct       60

attacaac                                                                68


<210>  36
<211>  3270
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleoide


<220>
<221>  misc_feature
<223>  Cas13b nucleotide sequence

<400>  36
atgaacatcc ccgctctggt ggaaaaccag aagaagtact ttggcaccta cagcgtgatg       60

gccatgctga acgctcagac cgtgctggac cacatccaga aggtggccga tattgagggc      120

gagcagaacg agaacaacga gaatctgtgg tttcaccccg tgatgagcca cctgtacaac      180

gccaagaacg gctacgacaa gcagcccgag aaaaccatgt tcatcatcga gcggctgcag      240

agctacttcc cattcctgaa gatcatggcc gagaaccaga gagagtacag caacggcaag      300

tacaagcaga accgcgtgga agtgaacagc aacgacatct tcgaggtgct gaagcgcgcc      360

ttcggcgtgc tgaagatgta cagggacctg accaaccact acaagaccta cgaggaaaag      420

ctgaacgacg gctgcgagtt cctgaccagc acagagcaac ctctgagcgg catgatcaac      480

aactactaca cagtggccct gcggaacatg aacgagagat acggctacaa gacagaggac      540

ctggccttca tccaggacaa gcggttcaag ttcgtgaagg acgcctacgg caagaaaaag      600

tcccaagtga ataccggatt cttcctgagc ctgcaggact acaacggcga cacacagaag      660

aagctgcacc tgagcggagt gggaatcgcc ctgctgatct gcctgttcct ggacaagcag      720

tacatcaaca tctttctgag caggctgccc atcttctcca gctacaatgc ccagagcgag      780

gaacggcgga tcatcatcag atccttcggc atcaacagca tcaagctgcc caaggaccgg      840

atccacagcg agaagtccaa caagagcgtg gccatggata tgctcaacga agtgaagcgg      900

tgccccgacg agctgttcac aacactgtct gccgagaagc agtcccggtt cagaatcatc      960

agcgacgacc acaatgaagt gctgatgaag cggagcagcg acagattcgt gcctctgctg     1020

ctgcagtata tcgattacgg caagctgttc gaccacatca ggttccacgt gaacatgggc     1080

aagctgagat acctgctgaa ggccgacaag acctgcatcg acggccagac cagagtcaga     1140

gtgatcgagc agcccctgaa cggcttcggc agactggaag aggccgagac aatgcggaag     1200

caagagaacg gcaccttcgg caacagcggc atccggatca gagacttcga gaacatgaag     1260

cgggacgacg ccaatcctgc caactatccc tacatcgtgg acacctacac acactacatc     1320

ctggaaaaca acaaggtcga gatgtttatc aacgacaaag aggacagcgc cccactgctg     1380

cccgtgatcg aggatgatag atacgtggtc aagacaatcc ccagctgccg gatgagcacc     1440

ctggaaattc cagccatggc cttccacatg tttctgttcg gcagcaagaa aaccgagaag     1500

ctgatcgtgg acgtgcacaa ccggtacaag agactgttcc aggccatgca gaaagaagaa     1560

gtgaccgccg agaatatcgc cagcttcgga atcgccgaga gcgacctgcc tcagaagatc     1620

ctggatctga tcagcggcaa tgcccacggc aaggatgtgg acgccttcat cagactgacc     1680

gtggacgaca tgctgaccga caccgagcgg agaatcaaga gattcaagga cgaccggaag     1740

tccattcgga gcgccgacaa caagatggga aagagaggct tcaagcagat ctccacaggc     1800

aagctggccg acttcctggc caaggacatc gtgctgtttc agcccagcgt gaacgatggc     1860

gagaacaaga tcaccggcct gaactaccgg atcatgcaga gcgccattgc cgtgtacgat     1920

agcggcgacg attacgaggc caagcagcag ttcaagctga tgttcgagaa ggcccggctg     1980

atcggcaagg gcacaacaga gcctcatcca tttctgtaca aggtgttcgc ccgcagcatc     2040

cccgccaatg ccgtcgagtt ctacgagcgc tacctgatcg agcggaagtt ctacctgacc     2100

ggcctgtcca acgagatcaa gaaaggcaac agagtggatg tgcccttcat ccggcgggac     2160

cagaacaagt ggaaaacacc cgccatgaag accctgggca gaatctacag cgaggatctg     2220

cccgtggaac tgcccagaca gatgttcgac aatgagatca agtcccacct gaagtccctg     2280

ccacagatgg aaggcatcga cttcaacaat gccaacgtga cctatctgat cgccgagtac     2340

atgaagagag tgctggacga cgacttccag accttctacc agtggaaccg caactaccgg     2400

tacatggaca tgcttaaggg cgagtacgac agaaagggct ccctgcagca ctgcttcacc     2460

agcgtggaag agagagaagg cctctggaaa gagcgggcct ccagaacaga gcggtacaga     2520

aagcaggcca gcaacaagat ccgcagcaac cggcagatga gaaacgccag cagcgaagag     2580

atcgagacaa tcctggataa gcggctgagc aacagccgga acgagtacca gaaaagcgag     2640

aaagtgatcc ggcgctacag agtgcaggat gccctgctgt ttctgctggc caaaaagacc     2700

ctgaccgaac tggccgattt cgacggcgag aggttcaaac tgaaagaaat catgcccgac     2760

gccgagaagg gaatcctgag cgagatcatg cccatgagct tcaccttcga gaaaggcggc     2820

aagaagtaca ccatcaccag cgagggcatg aagctgaaga actacggcga cttctttgtg     2880

ctggctagcg acaagaggat cggcaacctg ctggaactcg tgggcagcga catcgtgtcc     2940

aaagaggata tcatggaaga gttcaacaaa tacgaccagt gcaggcccga gatcagctcc     3000

atcgtgttca acctggaaaa gtgggccttc gacacatacc ccgagctgtc tgccagagtg     3060

gaccgggaag agaaggtgga cttcaagagc atcctgaaaa tcctgctgaa caacaagaac     3120

atcaacaaag agcagagcga catcctgcgg aagatccgga acgccttcga tcacaacaat     3180

taccccgaca aaggcgtggt ggaaatcaag gccctgcctg agatcgccat gagcatcaag     3240

aaggcctttg gggagtacgc catcatgaag                                      3270


<210>  37
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleoide


<220>
<221>  misc_feature
<223>  Cas13b direct repeat

<400>  37
gttgtggaag gtccagtttt gaggggctat tacaac                                 36


<210>  38
<211>  321
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleoide


<220>
<221>  misc_feature
<223>  gRNA1 expression cassette sequence

<400>  38
gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag       60

ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga      120

aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat      180

atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga      240

cgaaacaccg gcccttcgat tctgaaacca gatctgaatg ttgtggaagg tccagttttg      300

aggggctatt acaacttttt t                                                321


<210>  39
<211>  321
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleoide


<220>
<221>  misc_feature
<223>  gRNA2 expression cassette sequence

<400>  39
gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag       60

ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga      120

aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat      180

atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga      240

cgaaacaccg attctgaaac cagatctgaa tcctggactg ttgtggaagg tccagttttg      300

aggggctatt acaacttttt t                                                321


<210>  40
<211>  322
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleoide


<220>
<221>  misc_feature
<223>  gRNA3 expression cassette sequence

<400>  40
gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag       60

ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga      120

aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat      180

atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga      240

cgaaacaccg actccgggag gcccgtctct ctggccagct gttgtggaag gtccagtttt      300

gaggggctat tacaactttt tt                                               322


<210>  41
<211>  321
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleoide


<220>
<221>  misc_feature
<223>  gRNA4 expression cassette sequence

<400>  41
gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag       60

ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga      120

aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat      180

atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga      240

cgaaacaccg aggagcctga gggtgggaga gcgccccgtg ttgtggaagg tccagttttg      300

aggggctatt acaacttttt t                                                321


<210>  42
<211>  323
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleoide


<220>
<221>  misc_feature
<223>  gRNA5 expression cassette sequence

<400>  42
gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag       60

ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga      120

aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat      180

atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga      240

cgaaacaccg cttttgcccg ggtgcggagg ccaccgagga ggttgtggaa ggtccagttt      300

tgaggggcta ttacaacttt ttt                                              323


<210>  43
<211>  323
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleoide


<220>
<221>  misc_feature
<223>  gRNA6 expression cassette sequence

<400>  43
gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag       60

ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga      120

aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat      180

atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga      240

cgaaacaccg tctaggagag gttgcgcctg ctgcagaaac tgttgtggaa ggtccagttt      300

tgaggggcta ttacaacttt ttt                                              323


<210>  44
<211>  325
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleoide


<220>
<221>  misc_feature
<223>  gRNA7 expression cassette sequence

<400>  44
gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag       60

ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga      120

aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat      180

atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga      240

cgaaacaccg tttctaggag aggttgcgcc tgctgcagaa actgttgtgg aaggtccagt      300

tttgaggggc tattacaact ttttt                                            325


<210>  45
<211>  322
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleoide


<220>
<221>  misc_feature
<223>  gRNA8 expression cassette sequence

<400>  45
gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag       60

ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga      120

aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat      180

atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga      240

cgaaacaccg tggcagttct ccgcggtgtg gagtctctca gttgtggaag gtccagtttt      300

gaggggctat tacaactttt tt                                               322


<210>  46
<211>  321
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleoide


<220>
<221>  misc_feature
<223>  gRNA9 expression cassette sequence

<400>  46
gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag       60

ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga      120

aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat      180

atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga      240

cgaaacaccg ttctccgcgg tgtggagtct ctcaccgggg ttgtggaagg tccagttttg      300

aggggctatt acaacttttt t                                                321


<210>  47
<211>  321
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleoide


<220>
<221>  misc_feature
<223>  gRNA10 expression cassette sequence

<400>  47
gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag       60

ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga      120

aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat      180

atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga      240

cgaaacaccg aacaagggca cagagaggcc agcgagctcg ttgtggaagg tccagttttg      300

aggggctatt acaacttttt t                                                321


<210>  48
<211>  323
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleoide


<220>
<221>  misc_feature
<223>  gRNA11 expression cassette sequence

<400>  48
gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag       60

ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga      120

aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat      180

atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga      240

cgaaacaccg acagcgtcgg aaggtggggg gagacattca ggttgtggaa ggtccagttt      300

tgaggggcta ttacaacttt ttt                                              323


<210>  49
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleoide


<220>
<221>  misc_feature
<223>  gRNA12 sequence (control)

<400>  49
gcagggtttt cccagtcacg acgttgtaaa                                        30


<210>  50
<211>  322
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleoide


<220>
<221>  misc_feature
<223>  gRNA12 expression cassette sequence (control)

<400>  50
gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag       60

ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga      120

aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat      180

atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga      240

cgaaacaccg cagggttttc ccagtcacga cgttgtaaaa gttgtggaag gtccagtttt      300

gaggggctat tacaactttt tt                                               322


<210>  51
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleotide


<220>
<221>  misc_feature
<223>  gRNA13 sequence

<400>  51
gccagaattt cacggaagaa caagggcaca                                        30


<210>  52
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleotide


<220>
<221>  misc_feature
<223>  gRNA14 sequence

<400>  52
gctcccttgc acgtcagccg gggtgcgcac                                        30


<210>  53
<211>  30
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic Polynucleotide


<220>
<221>  misc_feature
<223>  gRNA15 sequence

<400>  53
gaaaccagat ctgaatcctg gactccggga                                        30


<210>  54
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleotide


<220>
<221>  misc_feature
<223>  gRNA16 sequence

<400>  54
gggtgcctgg cccttcgatt ctgaaaccag                                        30


<210>  55
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleotide


<220>
<221>  misc_feature
<223>  gRNA 13 targeting site

<400>  55
tgtgcccttg ttcttccgtg aaattctggc                                        30


<210>  56
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleotide


<220>
<221>  misc_feature
<223>  gRNA14 targeting site

<400>  56
gtgcgcaccc cggctgacgt gcaagggagc                                        30


<210>  57
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleotide


<220>
<221>  misc_feature
<223>  gRNA15 targeting site

<400>  57
tcccggagtc caggattcag atctggtttc                                        30


<210>  58
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleotide


<220>
<221>  misc_feature
<223>  gRNA16 targeting site

<400>  58
ctggtttcag aatcgaaggg ccaggcaccc                                        30


<210>  59
<211>  66
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleotide


<220>
<221>  misc_feature
<223>  gRNA13 + Cas13b direct repeat

<400>  59
gccagaattt cacggaagaa caagggcaca gttgtggaag gtccagtttt gaggggctat       60

tacaac                                                                  66


<210>  60
<211>  66
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleotide


<220>
<221>  misc_feature
<223>  gRNA14 + Cas13b direct repeat

<400>  60
gctcccttgc acgtcagccg gggtgcgcac gttgtggaag gtccagtttt gaggggctat       60

tacaac                                                                  66


<210>  61
<211>  66
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleotide


<220>
<221>  misc_feature
<223>  gRNA15 + Cas13b direct repeat

<400>  61
gaaaccagat ctgaatcctg gactccggga gttgtggaag gtccagtttt gaggggctat       60

tacaac                                                                  66


<210>  62
<211>  66
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleotide


<220>
<221>  misc_feature
<223>  gRNA16 + Cas13b direct repeat

<400>  62
gggtgcctgg cccttcgatt ctgaaaccag gttgtggaag gtccagtttt gaggggctat       60

tacaac                                                                  66


<210>  63
<211>  321
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleotide


<220>
<221>  misc_feature
<223>  gRNA13 expression cassette sequence

<400>  63
gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag       60

ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga      120

aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat      180

atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga      240

cgaaacaccg ccagaatttc acggaagaac aagggcacag ttgtggaagg tccagttttg      300

aggggctatt acaacttttt t                                                321


<210>  64
<211>  321
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleotide


<220>
<221>  misc_feature
<223>  gRNA14 expression cassette sequence

<400>  64
gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag       60

ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga      120

aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat      180

atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga      240

cgaaacaccg ctcccttgca cgtcagccgg ggtgcgcacg ttgtggaagg tccagttttg      300

aggggctatt acaacttttt t                                                321


<210>  65
<211>  321
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleotide


<220>
<221>  misc_feature
<223>  gRNA15 expression cassette sequence

<400>  65
gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag       60

ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga      120

aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat      180

atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga      240

cgaaacaccg aaaccagatc tgaatcctgg actccgggag ttgtggaagg tccagttttg      300

aggggctatt acaacttttt t                                                321


<210>  66
<211>  321
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleotide


<220>
<221>  misc_feature
<223>  gRNA16 expression cassette sequence

<400>  66
gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag       60

ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga      120

aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat      180

atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga      240

cgaaacaccg ggtgcctggc ccttcgattc tgaaaccagg ttgtggaagg tccagttttg      300

aggggctatt acaacttttt t                                                321


