                         SEQUENCE LISTING

<110>  F. Hoffmann-La Roche AG
       Hoffmann-La Roche Inc
 
<120>  Method for selecting cells based on CRISPR/Cas-mediated 
       integration of a detectable tag to a target protein

<130>  P35197-WO

<150>  EP18215918.6
<151>  2018-12-30

<160>  35    

<170>  PatentIn version 3.5

<210>  1
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA1 forward

<400>  1
gatcggagtg catctccatc cacgtg                                            26


<210>  2
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA1 reverse

<400>  2
aaaacacgtg gatggagatg cactcc                                            26


<210>  3
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA2 forward

<400>  3
gatcgggcca ggctggtgtc cagatg                                            26


<210>  4
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA2 reverse

<400>  4
aaaacatctg gacaccagcc tggccc                                            26


<210>  5
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA3 forward

<400>  5
gatcggagct ctactgcctg gaacag                                            26


<210>  6
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA3 reverse

<400>  6
aaaactgttc caggcagtag agctcc                                            26


<210>  7
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  crRNA1

<400>  7
gagtgcatct ccatccacgt                                                   20


<210>  8
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  crRNA2

<400>  8
ggccaggctg gtgtccagat                                                   20


<210>  9
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  crRNA3

<400>  9
gagctctact gcctggaaca                                                   20


<210>  10
<211>  55
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  D_Hygro fwd

<400>  10
cgaatgcggc cgcagaagca ataagaggac tgcggaagag ctccctgtca atgta            55


<210>  11
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  D_Hygro rev

<400>  11
gccttaatta aagtgctcca gggtggtgtg ggtggtgagg atggagttgt                  50


<210>  12
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  D_5HA fwd

<400>  12
gacattgatt attgaaagca ataagaggac tgcggaagag                             40


<210>  13
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  D_5HA rev

<400>  13
tgctcacctg cgggaaggaa aaaagatatc acaatttaaa                             40


<210>  14
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  D_eGFP fwd

<400>  14
tcccgcaggt gagcaagggc gaggaactgt tcaccggggt                             40


<210>  15
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  D_eGFP_rev

<400>  15
actcacggct gcctcccccg cctttgtaca gttcgtccat tccga                       45


<210>  16
<211>  80
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  D_3HA fwd

<400>  16
aaaggcgggg gaggcagccg tgaatgtata agtatacatg tgggacaagc aggagtacaa       60

atcggcaatg cctgctggga                                                   80


<210>  17
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  D_3HA rev

<400>  17
cttttgctca cggccagtgc tccagggtgg tgtgggtggt                             40


<210>  18
<211>  49
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  D_3HA-Z fwd

<400>  18
gtcatcaata gattggttta aattgtgata tcttttttcc ttcccgcag                   49


<210>  19
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  D_3'HA-Z rev

<400>  19
aaccagaaag ctttaacgtc tgtcagttaa gctgaagctg aaattctggg                  50


<210>  20
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  QuickChange fwd

<400>  20
tgcctgctgg gaattatatt gtttagagca tggcatccag                             40


<210>  21
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  QuickChange rev

<400>  21
ctggatgcca tgctctaaac aatataattc ccagcaggca                             40


<210>  22
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  eGFP fwd

<400>  22
ggccgacaag cagaaaaacg gcatcaaagt gaac                                   34


<210>  23
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TUBA1B Exon4 rev

<400>  23
ggcggttaag gttagtgtag gttggg                                            26


<210>  24
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  cDNA fwd

<400>  24
atggtgagca agggcgagga actgttcacc gggg                                   34


<210>  25
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  cDNA rev

<400>  25
ttagtattcc tctccttctt cctcaccc                                          28


<210>  26
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Hygro fwd

<400>  26
accgcaagga atcggtcaat                                                   20


<210>  27
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Hygro rev

<400>  27
tgctgctcca tacaagccaa                                                   20


<210>  28
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cas9 fwd

<400>  28
ccctgctgtt cgacagcggc gaaacagccg agg                                    33


<210>  29
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cas9 rev

<400>  29
ggcatcctcg gccaggtcga agttgctctt gaagttggg                              39


<210>  30
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  eGFP_2 fwd

<400>  30
gacctacggc gtgcagtgct tcagcagata ccc                                    33


<210>  31
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  eGFP_2 rev

<400>  31
gttcactttg atgccgtttt tctgcttgtc ggcc                                   34


<210>  32
<211>  239
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  eGFP-Tag

<400>  32

Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu 
1               5                   10                  15      


Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly 
            20                  25                  30          


Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile 
        35                  40                  45              


Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr 
    50                  55                  60                  


Leu Thr Tyr Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys 
65                  70                  75                  80  


Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu 
                85                  90                  95      


Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu 
            100                 105                 110         


Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly 
        115                 120                 125             


Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr 
    130                 135                 140                 


Asn Tyr Asn Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn 
145                 150                 155                 160 


Gly Ile Lys Val Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser 
                165                 170                 175     


Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly 
            180                 185                 190         


Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Tyr Gln Ser Ala Leu 
        195                 200                 205             


Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe 
    210                 215                 220                 


Val Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys 
225                 230                 235                 


<210>  33
<211>  717
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  eGFP-tag nuc

<400>  33
atggtgagca agggcgagga actgttcacc ggggtcgtgc ccatcctcgt tgagctggac       60

ggagatgtga acggccacaa attttccgtc tctggggaag gtgagggcga cgccacatac      120

ggaaagctta ctctgaaatt catttgcacc acagggaagt tgcctgtgcc atggcccact      180

ctcgtaacca cactgacgta tggcgtgcag tgttttagta gataccctga tcatatgaaa      240

cagcacgact ttttcaagag tgccatgcca gaaggttatg tgcaggagcg gacgatcttt      300

ttcaaggatg acggcaatta caaaaccaga gcagaggtca agtttgaagg ggatacactt      360

gtgaaccgca ttgagctgaa aggaatcgac ttcaaggaag atggcaatat actcgggcat      420

aaactggagt ataactacaa tagccacaac gtttacatca tggccgacaa gcagaagaat      480

ggtattaaag tgaacttcaa gatcaggcac aatattgagg acggctccgt ccaattggct      540

gatcattatc agcagaacac tcccatcgga gacgggcctg tgctgctccc agataatcac      600

tacctgtctt atcagtcagc acttagcaaa gacccgaacg aaaagcggga tcatatggtt      660

ctgttggagt ttgtaaccgc ggctggcata acactcggaa tggacgaact gtacaaa         717


<210>  34
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  34
guuuuagagc urugyuguuu ug                                                22


<210>  35
<211>  83
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  eGFP exaon 2 start

<400>  35
ctgagtgcat ctccatccac gttggccagg ctggtgtcca gattggcaat gcctgctggg       60

agctctactg cctggaacac ggc                                               83


