﻿                         序列表

<110>  浙江工业大学

<120>  一种氨基酸脱氢酶突变体及其在合成L-草铵膦中的应用

<160>  4

<170>  SIPOSequenceListing 1.0

<210>  1
<211>  1355
<212>  DNA
<213>  蒙氏假单胞菌(Pseudomonas monteilii)

<400>  1
gagctcatga ttgaaagcgt cgactcattt ttagcacgct tacaacaacg cgatccaggt        60
caacctgagt ttcaccaagc cgtggaggaa gttctgcgca cgttgtggcc gtttctggaa       120
gccaacccgc gctatttgca aagcggtatc ctggaacgta tggtcgagcc agaacgtgct       180
gtcttgtttc gcgtaagctg ggtagacgac caagggaaag ttcaggtgaa ccgtggttat       240
cgtattcaaa tgagcagcgc gattggaccc tataagggag gtttgcgttt tcacccttcc       300
gtgaatctga gtgtactgaa attcttggcc tttgaacaag tttttaaaaa ttcgctgact       360
tcccttccta tgggaggggg taagggggga tctgattttg atccgaaggg taaatctgac       420
gcggaggtaa tgcgcttttg ccaggcgttt atgtcggagt tgtaccgcca cattggggcg       480
gactgcgacg tgccagcggg ggatattgga gtaggagccc gcgagattgg gtttatgttc       540
ggacaataca agcgtttggc aaatcaattc acatcggttt tgaccgggaa agggatgacg       600
tatgggggca gtttaattcg tcctgaagca acgggatacg gttgcgtata cttcgcggag       660
gaaatgctta aacgtcaagg ccttcgcgta gatggccgcc gcgttgctat cagcggctct       720
gggaacgtcg ctcagtatgc agcgcgcaaa gtgatggact tgggcggcaa agtcatctct       780
ctttccgatt cggaaggtac cttatatgca gagggtgggt tgaccgaagc acaatgggaa       840
gcggtgatgc aattgaaaaa cgtagcgcgc ggacgcattt cagagcttgc tgaagccttc       900
ggtctggaat tccgcaaggg ccagactccg tggagcttac catgtgacat tgcattgccg       960
tgtgcgacgc aaaatgagct gggtatcgaa gatgcgcgca cccttcttcg caatggttgt      1020
atttgcgtgg ctgagggagc taatatgccg acgacgttgg ccgcggtgga cctgttcatc      1080
gacgctggta tcctttatgc accgggaaag gcaagtaacg ctggcggtgt ggccgtttcg      1140
gggttagaga tgagtcaaaa cgcgatgcgt cttctttgga cagcgggcga agtcgatagc      1200
aagttgcata atattatgca gtccattcac catgcctgtg ttcactatgg tgaggaggct      1260
gacggaaaag tgaattacgt taaaggagct aacattgcgg gctttgttaa agtcgccgac      1320
gcaatgcttg cccagggagt agtctaagcg gccgc                                 1355

<210>  2
<211>  446
<212>  PRT
<213>  蒙氏假单胞菌(Pseudomonas monteilii)

<400>  2
Met Ile Glu Ser Val Asp Ser Phe Leu Ala Arg Leu Gln Gln Arg Asp 
1               5                   10                  15      
Pro Gly Gln Pro Glu Phe His Gln Ala Val Glu Glu Val Leu Arg Thr 
            20                  25                  30          
Leu Trp Pro Phe Leu Glu Ala Asn Pro Arg Tyr Leu Gln Ser Gly Ile 
        35                  40                  45              
Leu Glu Arg Met Val Glu Pro Glu Arg Ala Val Leu Phe Arg Val Ser 
    50                  55                  60                  
Trp Val Asp Asp Gln Gly Lys Val Gln Val Asn Arg Gly Tyr Arg Ile 
65                  70                  75                  80  
Gln Met Ser Ser Ala Ile Gly Pro Tyr Lys Gly Gly Leu Arg Phe His 
                85                  90                  95      
Pro Ser Val Asn Leu Ser Val Leu Lys Phe Leu Ala Phe Glu Gln Val 
            100                 105                 110         
Phe Lys Asn Ser Leu Thr Ser Leu Pro Met Gly Gly Gly Lys Gly Gly 
        115                 120                 125             
Ser Asp Phe Asp Pro Lys Gly Lys Ser Asp Ala Glu Val Met Arg Phe 
    130                 135                 140                 
Cys Gln Ala Phe Met Ser Glu Leu Tyr Arg His Ile Gly Ala Asp Cys 
145                 150                 155                 160 
Asp Val Pro Ala Gly Asp Ile Gly Val Gly Ala Arg Glu Ile Gly Phe 
                165                 170                 175     
Met Phe Gly Gln Tyr Lys Arg Leu Ala Asn Gln Phe Thr Ser Val Leu 
            180                 185                 190         
Thr Gly Lys Gly Met Thr Tyr Gly Gly Ser Leu Ile Arg Pro Glu Ala 
        195                 200                 205             
Thr Gly Tyr Gly Cys Val Tyr Phe Ala Glu Glu Met Leu Lys Arg Gln 
    210                 215                 220                 
Gly Leu Arg Val Asp Gly Arg Arg Val Ala Ile Ser Gly Ser Gly Asn 
225                 230                 235                 240 
Val Ala Gln Tyr Ala Ala Arg Lys Val Met Asp Leu Gly Gly Lys Val 
                245                 250                 255     
Ile Ser Leu Ser Asp Ser Glu Gly Thr Leu Tyr Ala Glu Gly Gly Leu 
            260                 265                 270         
Thr Glu Ala Gln Trp Glu Ala Val Met Gln Leu Lys Asn Val Ala Arg 
        275                 280                 285             
Gly Arg Ile Ser Glu Leu Ala Glu Ala Phe Gly Leu Glu Phe Arg Lys 
    290                 295                 300                 
Gly Gln Thr Pro Trp Ser Leu Pro Cys Asp Ile Ala Leu Pro Cys Ala 
305                 310                 315                 320 
Thr Gln Asn Glu Leu Gly Ile Glu Asp Ala Arg Thr Leu Leu Arg Asn 
                325                 330                 335     
Gly Cys Ile Cys Val Ala Glu Gly Ala Asn Met Pro Thr Thr Leu Ala 
            340                 345                 350         
Ala Val Asp Leu Phe Ile Asp Ala Gly Ile Leu Tyr Ala Pro Gly Lys 
        355                 360                 365             
Ala Ser Asn Ala Gly Gly Val Ala Val Ser Gly Leu Glu Met Ser Gln 
    370                 375                 380                 
Asn Ala Met Arg Leu Leu Trp Thr Ala Gly Glu Val Asp Ser Lys Leu 
385                 390                 395                 400 
His Asn Ile Met Gln Ser Ile His His Ala Cys Val His Tyr Gly Glu 
                405                 410                 415     
Glu Ala Asp Gly Lys Val Asn Tyr Val Lys Gly Ala Asn Ile Ala Gly 
            420                 425                 430         
Phe Val Lys Val Ala Asp Ala Met Leu Ala Gln Gly Val Val 
        435                 440                 445     

<210>  3
<211>  789
<212>  DNA
<213>  微小杆菌(Exiguobacteriumsibiricum)

<400>  3
atgggttata attctctgaa aggcaaagtc gcgattgtta ctggtggtag catgggcatt        60
ggcgaagcga tcatccgtcg ctatgcagaa gaaggcatgc gcgttgttat caactatcgt       120
agccatccgg aggaagccaa aaagatcgcc gaagatatta aacaggcagg tggtgaagcc       180
ctgaccgtcc agggtgacgt ttctaaagag gaagacatga tcaacctggt gaaacagact       240
gttgatcact tcggtcagct ggacgtcttt gtgaacaacg ctggcgttga gatgccttct       300
ccgtcccacg aaatgtccct ggaagactgg cagaaagtga tcgatgttaa tctgacgggt       360
gcgttcctgg gcgctcgtga agctctgaaa tacttcgttg aacataacgt gaaaggcaac       420
attatcaata tgtctagcgt ccacgaaatc atcccgtggc ctactttcgt acattacgct       480
gcttctaagg gtggcgttaa actgatgacc cagactctgg ctatggaata tgcaccgaaa       540
ggtatccgca ttaacgctat cggtccaggc gcgatcaaca ctccaattaa tgcagaaaaa       600
ttcgaggatc cgaaacagcg tgcagacgtg gaaagcatga tcccgatggg caacatcggc       660
aagccagagg agatttccgc tgtcgcggca tggctggctt ctgacgaagc gtcttacgtt       720
accggcatca ccctgttcgc agatggtggc atgaccctgt acccgagctt tcaggctggc       780
cgtggttga                                                               789

<210>  4
<211>  262
<212>  PRT
<213>  微小杆菌(Exiguobacteriumsibiricum)

<400>  4
Met Gly Tyr Asn Ser Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly 
1               5                   10                  15      
Ser Met Gly Ile Gly Glu Ala Ile Ile Arg Arg Tyr Ala Glu Glu Gly 
            20                  25                  30          
Met Arg Val Val Ile Asn Tyr Arg Ser His Pro Glu Glu Ala Lys Lys 
        35                  40                  45              
Ile Ala Glu Asp Ile Lys Gln Ala Gly Gly Glu Ala Leu Thr Val Gln 
    50                  55                  60                  
Gly Asp Val Ser Lys Glu Glu Asp Met Ile Asn Leu Val Lys Gln Thr 
65                  70                  75                  80  
Val Asp His Phe Gly Gln Leu Asp Val Phe Val Asn Asn Ala Gly Val 
                85                  90                  95      
Glu Met Pro Ser Pro Ser His Glu Met Ser Leu Glu Asp Trp Gln Lys 
            100                 105                 110         
Val Ile Asp Val Asn Leu Thr Gly Ala Phe Leu Gly Ala Arg Glu Ala 
        115                 120                 125             
Leu Lys Tyr Phe Val Glu His Asn Val Lys Gly Asn Ile Ile Asn Met 
    130                 135                 140                 
Ser Ser Val His Glu Ile Ile Pro Trp Pro Thr Phe Val His Tyr Ala 
145                 150                 155                 160 
Ala Ser Lys Gly Gly Val Lys Leu Met Thr Gln Thr Leu Ala Met Glu 
                165                 170                 175     
Tyr Ala Pro Lys Gly Ile Arg Ile Asn Ala Ile Gly Pro Gly Ala Ile 
            180                 185                 190         
Asn Thr Pro Ile Asn Ala Glu Lys Phe Glu Asp Pro Lys Gln Arg Ala 
        195                 200                 205             
Asp Val Glu Ser Met Ile Pro Met Gly Asn Ile Gly Lys Pro Glu Glu 
    210                 215                 220                 
Ile Ser Ala Val Ala Ala Trp Leu Ala Ser Asp Glu Ala Ser Tyr Val 
225                 230                 235                 240 
Thr Gly Ile Thr Leu Phe Ala Asp Gly Gly Met Thr Leu Tyr Pro Ser 
                245                 250                 255     
Phe Gln Ala Gly Arg Gly 
            260         

