                         SEQUENCE LISTING

<110>  The Wistar Institute of Anatomy and Biology
       Muthumani, Kar
       Weiner, David
       Choi, Hyeree
       Kudchodkar, Sagar
 
<120>  VACCINES AGAINST POWASSAN VIRUS, AND METHODS OF USING SAME

<130>  206193-0022-00WO

<150>  US 62/782,407
<151>  2018-12-20

<150>  US 62/885,405
<151>  2019-08-12

<160>  13    

<170>  PatentIn version 3.5

<210>  1
<211>  387
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, consensus POWV capsid immunogen

<400>  1
gtgactacat caaaggggaa gggaggaggg cctcctagga gaaagctgaa ggtgaccgcc       60

aacaagagcc gcccagccac atccccaatg cccaagggct tcgtgctgag caggatgctg      120

ggcatcctgt ggcacgcagt gaccggaaca gccaggcccc ctgtgctgaa gatgttttgg      180

aagaccgtgc cactgagaca ggcagaggcc gccctgaaga agatcaagag agtgatcggc      240

aatctgatgc agtctctgca catgagaggc cggcgcagga gcggagtgga ctggacctgg      300

acattcctga ccatggccct gatgacaatg gcaatggcta caactattca cagagacaga      360

gagggctata tggtcatgcg ggcaagc                                          387


<210>  2
<211>  129
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, consensus POWV capsid immunogen

<400>  2

Val Thr Thr Ser Lys Gly Lys Gly Gly Gly Pro Pro Arg Arg Lys Leu 
1               5                   10                  15      


Lys Val Thr Ala Asn Lys Ser Arg Pro Ala Thr Ser Pro Met Pro Lys 
            20                  25                  30          


Gly Phe Val Leu Ser Arg Met Leu Gly Ile Leu Trp His Ala Val Thr 
        35                  40                  45              


Gly Thr Ala Arg Pro Pro Val Leu Lys Met Phe Trp Lys Thr Val Pro 
    50                  55                  60                  


Leu Arg Gln Ala Glu Ala Ala Leu Lys Lys Ile Lys Arg Val Ile Gly 
65                  70                  75                  80  


Asn Leu Met Gln Ser Leu His Met Arg Gly Arg Arg Arg Ser Gly Val 
                85                  90                  95      


Asp Trp Thr Trp Thr Phe Leu Thr Met Ala Leu Met Thr Met Ala Met 
            100                 105                 110         


Ala Thr Thr Ile His Arg Asp Arg Glu Gly Tyr Met Val Met Arg Ala 
        115                 120                 125             


Ser 
    


<210>  3
<211>  447
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, consensus POWV capsid immunogen operably 
       linked to a sequence encoding an IgE leader

<400>  3
atggactgga cttggattct gtttctggtc gccgcagcaa cccgcgtgca tagcgtgact       60

acatcaaagg ggaagggagg agggcctcct aggagaaagc tgaaggtgac cgccaacaag      120

agccgcccag ccacatcccc aatgcccaag ggcttcgtgc tgagcaggat gctgggcatc      180

ctgtggcacg cagtgaccgg aacagccagg ccccctgtgc tgaagatgtt ttggaagacc      240

gtgccactga gacaggcaga ggccgccctg aagaagatca agagagtgat cggcaatctg      300

atgcagtctc tgcacatgag aggccggcgc aggagcggag tggactggac ctggacattc      360

ctgaccatgg ccctgatgac aatggcaatg gctacaacta ttcacagaga cagagagggc      420

tatatggtca tgcgggcaag ctaatga                                          447


<210>  4
<211>  147
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, consensus POWV capsid immunogen operably 
       linked to  an IgE leader

<400>  4

Met Asp Trp Thr Trp Ile Leu Phe Leu Val Ala Ala Ala Thr Arg Val 
1               5                   10                  15      


His Ser Val Thr Thr Ser Lys Gly Lys Gly Gly Gly Pro Pro Arg Arg 
            20                  25                  30          


Lys Leu Lys Val Thr Ala Asn Lys Ser Arg Pro Ala Thr Ser Pro Met 
        35                  40                  45              


Pro Lys Gly Phe Val Leu Ser Arg Met Leu Gly Ile Leu Trp His Ala 
    50                  55                  60                  


Val Thr Gly Thr Ala Arg Pro Pro Val Leu Lys Met Phe Trp Lys Thr 
65                  70                  75                  80  


Val Pro Leu Arg Gln Ala Glu Ala Ala Leu Lys Lys Ile Lys Arg Val 
                85                  90                  95      


Ile Gly Asn Leu Met Gln Ser Leu His Met Arg Gly Arg Arg Arg Ser 
            100                 105                 110         


Gly Val Asp Trp Thr Trp Thr Phe Leu Thr Met Ala Leu Met Thr Met 
        115                 120                 125             


Ala Met Ala Thr Thr Ile His Arg Asp Arg Glu Gly Tyr Met Val Met 
    130                 135                 140                 


Arg Ala Ser 
145         


<210>  5
<211>  1347
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, consensus POWV envelope immunogen

<400>  5
gggagagacg cagcaagtca ggtccgggtg cagaacggca cctgcgtgat cctggccaca       60

gacatgggcg agtggtgcga ggattctatc acctacagct gcgtgacaat cgaccaggag      120

gaggagcccg tggacgtgga ttgcttctgt aggggcgtgg atagagtgaa gctggagtat      180

ggcaggtgtg gcagacaggc cggctttcgg ggcaagagga gcgtggtcat ccctacccac      240

gcacagaagg acatggtggg aaggggacac gcatggctga agggcgacaa catccgggat      300

cacgtgaccc gcgtggaggg atggatgtgg aagaataagc tgctgacagt ggccatcgtg      360

gccctggcct ggctgatggt ggactcttgg atggccaggg tgacagtgat cctgctggcc      420

ctgagcctgg gacccgtgta cgccacccgg tgcacacacc tggagaaccg cgatttcgtg      480

accggcacac agggcaccac aagagtgtcc ctggtgctgg agctgggcgg ctgcgtgacc      540

atcacagcag agggcaagcc aagcatcgac gtgtggctgg aggatatctt tcaggagtcc      600

cccgccgaga caagggagta ttgcctgcac gccaagctga ccaatacaaa ggtggaggca      660

aggtgtccaa ccacaggacc agccacactg cctgaggagc accaggccaa catggtgtgc      720

aagagggacc agtccgatag aggctggggc aatcactgtg gcttctttgg caagggctct      780

atcgtggcct gcgccaagtt cgagtgtgag gaggccaaga aggccgtggg ccacgtgtac      840

gacagcacca agatcacata tgtggtgaag gtggagccac acaccggcga ttacctggcc      900

gccaacgaga caaactccaa taggaagtct gcccagttta ccgtggcctc cgagaaagtg      960

atcctgagac tgggcgacta tggcgatgtg agcctgacct gcaaggtggc atccggaatc     1020

gacgtggcac agacagtggt catgtccctg ggcagctcca aggatcacct gccttctgcc     1080

tggcaggtgc accgcgactg gttcgaggat ctggccctgc catggaagca caaggacaac     1140

caggattgga atagcgtgga gaagctggtg gagttcggcc ctccccacgc agtgaagatg     1200

gacgtgttta atctgggcga tcagaccgcc gtgctgctga agtctctggc aggagtgcca     1260

ctggcaagcg tggagggcca gaagtaccac ctgaaaagcg ggcatgtgac ttgcgatgtc     1320

ggactggaaa aactgaaact gaaaggc                                         1347


<210>  6
<211>  448
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, consensus POWV envelope immunogen

<400>  6

Gly Arg Asp Ala Ala Ser Gln Val Arg Val Gln Asn Gly Thr Cys Val 
1               5                   10                  15      


Ile Leu Ala Thr Asp Met Gly Glu Trp Cys Glu Asp Ser Ile Thr Tyr 
            20                  25                  30          


Ser Cys Val Thr Ile Asp Gln Glu Glu Pro Val Asp Val Asp Cys Phe 
        35                  40                  45              


Cys Arg Gly Val Asp Arg Val Lys Leu Glu Tyr Gly Arg Cys Gly Arg 
    50                  55                  60                  


Gln Ala Gly Phe Arg Gly Lys Arg Ser Val Val Ile Pro Thr His Ala 
65                  70                  75                  80  


Gln Lys Asp Met Val Gly Arg Gly His Ala Trp Leu Lys Gly Asp Asn 
                85                  90                  95      


Ile Arg Asp His Val Thr Arg Val Glu Gly Trp Met Trp Lys Asn Lys 
            100                 105                 110         


Leu Leu Thr Val Ala Ile Val Ala Leu Ala Trp Leu Met Val Asp Ser 
        115                 120                 125             


Trp Met Ala Arg Val Thr Val Ile Leu Leu Ala Leu Ser Leu Gly Pro 
    130                 135                 140                 


Val Tyr Ala Thr Arg Cys Thr His Leu Glu Asn Arg Asp Phe Val Thr 
145                 150                 155                 160 


Gly Thr Gln Gly Thr Thr Arg Val Ser Leu Val Leu Glu Leu Gly Gly 
                165                 170                 175     


Cys Val Thr Ile Thr Ala Glu Gly Lys Pro Ser Ile Asp Val Trp Leu 
            180                 185                 190         


Glu Asp Ile Phe Gln Glu Ser Pro Ala Glu Thr Arg Glu Tyr Cys Leu 
        195                 200                 205             


His Ala Lys Leu Thr Asn Thr Lys Val Glu Ala Arg Cys Pro Thr Thr 
    210                 215                 220                 


Gly Pro Ala Thr Leu Pro Glu Glu His Gln Ala Asn Met Val Cys Lys 
225                 230                 235                 240 


Arg Asp Gln Ser Asp Arg Gly Trp Gly Asn His Cys Gly Phe Phe Gly 
                245                 250                 255     


Lys Gly Ser Ile Val Ala Cys Ala Lys Phe Glu Cys Glu Glu Ala Lys 
            260                 265                 270         


Lys Ala Val Gly His Val Tyr Asp Ser Thr Lys Ile Thr Tyr Val Val 
        275                 280                 285             


Lys Val Glu Pro His Thr Gly Asp Tyr Leu Ala Ala Asn Glu Thr Asn 
    290                 295                 300                 


Ser Asn Arg Lys Ser Ala Gln Phe Thr Val Ala Ser Glu Lys Val Ile 
305                 310                 315                 320 


Leu Arg Leu Gly Asp Tyr Gly Asp Val Ser Leu Thr Cys Lys Val Ala 
                325                 330                 335     


Ser Gly Ile Asp Val Ala Gln Thr Val Val Met Ser Leu Gly Ser Ser 
            340                 345                 350         


Lys Asp His Leu Pro Ser Ala Trp Gln Val His Arg Asp Trp Phe Glu 
        355                 360                 365             


Asp Leu Ala Leu Pro Trp Lys His Lys Asp Asn Gln Asp Trp Asn Ser 
    370                 375                 380                 


Val Glu Lys Leu Val Glu Phe Gly Pro Pro His Ala Val Lys Met Asp 
385                 390                 395                 400 


Val Phe Asn Leu Gly Asp Gln Thr Ala Val Leu Leu Lys Ser Leu Ala 
                405                 410                 415     


Gly Val Pro Leu Ala Ser Val Glu Gly Gln Lys Tyr His Leu Lys Ser 
            420                 425                 430         


Gly His Val Thr Cys Asp Val Gly Leu Glu Lys Leu Lys Leu Lys Gly 
        435                 440                 445             


<210>  7
<211>  1407
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, a consensus POWV envelope immunogen 
       operably linked to a sequence encoding an IgE leader

<400>  7
atggattgga catggattct gtttctggtc gcagccgcca cacgagtgca ttcagggaga       60

gacgcagcaa gtcaggtccg ggtgcagaac ggcacctgcg tgatcctggc cacagacatg      120

ggcgagtggt gcgaggattc tatcacctac agctgcgtga caatcgacca ggaggaggag      180

cccgtggacg tggattgctt ctgtaggggc gtggatagag tgaagctgga gtatggcagg      240

tgtggcagac aggccggctt tcggggcaag aggagcgtgg tcatccctac ccacgcacag      300

aaggacatgg tgggaagggg acacgcatgg ctgaagggcg acaacatccg ggatcacgtg      360

acccgcgtgg agggatggat gtggaagaat aagctgctga cagtggccat cgtggccctg      420

gcctggctga tggtggactc ttggatggcc agggtgacag tgatcctgct ggccctgagc      480

ctgggacccg tgtacgccac ccggtgcaca cacctggaga accgcgattt cgtgaccggc      540

acacagggca ccacaagagt gtccctggtg ctggagctgg gcggctgcgt gaccatcaca      600

gcagagggca agccaagcat cgacgtgtgg ctggaggata tctttcagga gtcccccgcc      660

gagacaaggg agtattgcct gcacgccaag ctgaccaata caaaggtgga ggcaaggtgt      720

ccaaccacag gaccagccac actgcctgag gagcaccagg ccaacatggt gtgcaagagg      780

gaccagtccg atagaggctg gggcaatcac tgtggcttct ttggcaaggg ctctatcgtg      840

gcctgcgcca agttcgagtg tgaggaggcc aagaaggccg tgggccacgt gtacgacagc      900

accaagatca catatgtggt gaaggtggag ccacacaccg gcgattacct ggccgccaac      960

gagacaaact ccaataggaa gtctgcccag tttaccgtgg cctccgagaa agtgatcctg     1020

agactgggcg actatggcga tgtgagcctg acctgcaagg tggcatccgg aatcgacgtg     1080

gcacagacag tggtcatgtc cctgggcagc tccaaggatc acctgccttc tgcctggcag     1140

gtgcaccgcg actggttcga ggatctggcc ctgccatgga agcacaagga caaccaggat     1200

tggaatagcg tggagaagct ggtggagttc ggccctcccc acgcagtgaa gatggacgtg     1260

tttaatctgg gcgatcagac cgccgtgctg ctgaagtctc tggcaggagt gccactggca     1320

agcgtggagg gccagaagta ccacctgaaa agcgggcatg tgacttgcga tgtcggactg     1380

gaaaaactga aactgaaagg ctaatga                                         1407


<210>  8
<211>  466
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, a consensus POWV envelope immunogen 
       operably linked to an IgE leader

<400>  8

Met Asp Trp Thr Trp Ile Leu Phe Leu Val Ala Ala Ala Thr Arg Val 
1               5                   10                  15      


His Ser Gly Arg Asp Ala Ala Ser Gln Val Arg Val Gln Asn Gly Thr 
            20                  25                  30          


Cys Val Ile Leu Ala Thr Asp Met Gly Glu Trp Cys Glu Asp Ser Ile 
        35                  40                  45              


Thr Tyr Ser Cys Val Thr Ile Asp Gln Glu Glu Pro Val Asp Val Asp 
    50                  55                  60                  


Cys Phe Cys Arg Gly Val Asp Arg Val Lys Leu Glu Tyr Gly Arg Cys 
65                  70                  75                  80  


Gly Arg Gln Ala Gly Phe Arg Gly Lys Arg Ser Val Val Ile Pro Thr 
                85                  90                  95      


His Ala Gln Lys Asp Met Val Gly Arg Gly His Ala Trp Leu Lys Gly 
            100                 105                 110         


Asp Asn Ile Arg Asp His Val Thr Arg Val Glu Gly Trp Met Trp Lys 
        115                 120                 125             


Asn Lys Leu Leu Thr Val Ala Ile Val Ala Leu Ala Trp Leu Met Val 
    130                 135                 140                 


Asp Ser Trp Met Ala Arg Val Thr Val Ile Leu Leu Ala Leu Ser Leu 
145                 150                 155                 160 


Gly Pro Val Tyr Ala Thr Arg Cys Thr His Leu Glu Asn Arg Asp Phe 
                165                 170                 175     


Val Thr Gly Thr Gln Gly Thr Thr Arg Val Ser Leu Val Leu Glu Leu 
            180                 185                 190         


Gly Gly Cys Val Thr Ile Thr Ala Glu Gly Lys Pro Ser Ile Asp Val 
        195                 200                 205             


Trp Leu Glu Asp Ile Phe Gln Glu Ser Pro Ala Glu Thr Arg Glu Tyr 
    210                 215                 220                 


Cys Leu His Ala Lys Leu Thr Asn Thr Lys Val Glu Ala Arg Cys Pro 
225                 230                 235                 240 


Thr Thr Gly Pro Ala Thr Leu Pro Glu Glu His Gln Ala Asn Met Val 
                245                 250                 255     


Cys Lys Arg Asp Gln Ser Asp Arg Gly Trp Gly Asn His Cys Gly Phe 
            260                 265                 270         


Phe Gly Lys Gly Ser Ile Val Ala Cys Ala Lys Phe Glu Cys Glu Glu 
        275                 280                 285             


Ala Lys Lys Ala Val Gly His Val Tyr Asp Ser Thr Lys Ile Thr Tyr 
    290                 295                 300                 


Val Val Lys Val Glu Pro His Thr Gly Asp Tyr Leu Ala Ala Asn Glu 
305                 310                 315                 320 


Thr Asn Ser Asn Arg Lys Ser Ala Gln Phe Thr Val Ala Ser Glu Lys 
                325                 330                 335     


Val Ile Leu Arg Leu Gly Asp Tyr Gly Asp Val Ser Leu Thr Cys Lys 
            340                 345                 350         


Val Ala Ser Gly Ile Asp Val Ala Gln Thr Val Val Met Ser Leu Gly 
        355                 360                 365             


Ser Ser Lys Asp His Leu Pro Ser Ala Trp Gln Val His Arg Asp Trp 
    370                 375                 380                 


Phe Glu Asp Leu Ala Leu Pro Trp Lys His Lys Asp Asn Gln Asp Trp 
385                 390                 395                 400 


Asn Ser Val Glu Lys Leu Val Glu Phe Gly Pro Pro His Ala Val Lys 
                405                 410                 415     


Met Asp Val Phe Asn Leu Gly Asp Gln Thr Ala Val Leu Leu Lys Ser 
            420                 425                 430         


Leu Ala Gly Val Pro Leu Ala Ser Val Glu Gly Gln Lys Tyr His Leu 
        435                 440                 445             


Lys Ser Gly His Val Thr Cys Asp Val Gly Leu Glu Lys Leu Lys Leu 
    450                 455                 460                 


Lys Gly 
465     


<210>  9
<211>  1761
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, consensus POWV capsid and envelope 
       immunogen

<400>  9
gtgactactt caaaaggcaa aggaggaggc ccccccagga gaaagctgaa ggtgaccgcc       60

aacaagagcc gccctgccac atcccctatg ccaaagggct tcgtgctgag caggatgctg      120

ggcatcctgt ggcacgcagt gaccggaaca gccagacccc ctgtgctgaa gatgttttgg      180

aagaccgtgc ctctgagaca ggcagaggcc gccctgaaga agatcaagag agtgatcggc      240

aatctgatgc agtctctgca catgaggggc cggcgcagga gcggagtgga ctggacctgg      300

acattcctga ccatggccct gatgacaatg gccatggcca ccacaatcca ccgcgatagg      360

gagggctaca tggtcatgag agcctctaga ggcaggaaga gacggagcgc caccggaaga      420

gacgcagcat ctcaggtgcg ggtgcagaac ggcacctgcg tgatcctggc cacagacatg      480

ggcgagtggt gcgaggattc tatcacctac agctgcgtga caatcgacca ggaggaggag      540

cccgtggacg tggattgctt ctgtcgcggc gtggataggg tgaagctgga gtatggccgc      600

tgtggcaggc aggcaggctt tagaggcaag cggagcgtgg tcatccctac ccacgcacag      660

aaggacatgg tgggaagggg acacgcatgg ctgaagggcg acaacatcag agatcacgtg      720

acccgggtgg agggctggat gtggaagaat aagctgctga cagtggccat cgtggccctg      780

gcctggctga tggtggattc ttggatggcc cgggtgacag tgatcctgct ggccctgagc      840

ctgggacccg tgtacgccac ccgctgcaca cacctggaga acagggactt cgtgaccgga      900

acacagggaa ccacacgcgt gtccctggtg ctggagctgg gcggctgcgt gaccatcaca      960

gcagagggca agccaagcat cgacgtgtgg ctggaggata tctttcagga gtcccccgcc     1020

gagacaaggg agtattgcct gcacgccaag ctgaccaata caaaggtgga ggccagatgt     1080

cccaccacag gccctgccac actgccagag gagcaccagg ccaacatggt gtgcaagcgc     1140

gaccagtccg ataggggctg gggcaatcac tgtggcttct ttggcaaggg ctctatcgtg     1200

gcctgcgcca agttcgagtg tgaggaggcc aagaaggccg tgggccacgt gtacgacagc     1260

accaagatca catatgtggt gaaggtggag ccacacaccg gcgattacct ggccgccaac     1320

gagacaaact ccaatcggaa gtctgcccag tttaccgtgg cctccgagaa agtgatcctg     1380

cgcctgggcg actatggcga cgtgagcctg acctgcaagg tggcctctgg catcgacgtg     1440

gcccagacag tggtcatgtc cctgggcagc tccaaggatc acctgccttc tgcctggcag     1500

gtgcaccgcg actggtttga ggatctggcc ctgccatgga agcacaagga caaccaggat     1560

tggaattctg tggagaagct ggtggagttc ggcccacccc acgcagtgaa gatggacgtg     1620

tttaatctgg gcgatcagac cgccgtgctg ctgaagagcc tggcaggagt gccactggca     1680

tccgtggagg gccagaagta tcacctgaaa agcgggcatg tgacctgcga tgtggggctg     1740

gaaaaactga aactgaaagg g                                               1761


<210>  10
<211>  587
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, consensus POWV capsid and envelope 
       immunogen

<400>  10

Val Thr Thr Ser Lys Gly Lys Gly Gly Gly Pro Pro Arg Arg Lys Leu 
1               5                   10                  15      


Lys Val Thr Ala Asn Lys Ser Arg Pro Ala Thr Ser Pro Met Pro Lys 
            20                  25                  30          


Gly Phe Val Leu Ser Arg Met Leu Gly Ile Leu Trp His Ala Val Thr 
        35                  40                  45              


Gly Thr Ala Arg Pro Pro Val Leu Lys Met Phe Trp Lys Thr Val Pro 
    50                  55                  60                  


Leu Arg Gln Ala Glu Ala Ala Leu Lys Lys Ile Lys Arg Val Ile Gly 
65                  70                  75                  80  


Asn Leu Met Gln Ser Leu His Met Arg Gly Arg Arg Arg Ser Gly Val 
                85                  90                  95      


Asp Trp Thr Trp Thr Phe Leu Thr Met Ala Leu Met Thr Met Ala Met 
            100                 105                 110         


Ala Thr Thr Ile His Arg Asp Arg Glu Gly Tyr Met Val Met Arg Ala 
        115                 120                 125             


Ser Arg Gly Arg Lys Arg Arg Ser Ala Thr Gly Arg Asp Ala Ala Ser 
    130                 135                 140                 


Gln Val Arg Val Gln Asn Gly Thr Cys Val Ile Leu Ala Thr Asp Met 
145                 150                 155                 160 


Gly Glu Trp Cys Glu Asp Ser Ile Thr Tyr Ser Cys Val Thr Ile Asp 
                165                 170                 175     


Gln Glu Glu Glu Pro Val Asp Val Asp Cys Phe Cys Arg Gly Val Asp 
            180                 185                 190         


Arg Val Lys Leu Glu Tyr Gly Arg Cys Gly Arg Gln Ala Gly Phe Arg 
        195                 200                 205             


Gly Lys Arg Ser Val Val Ile Pro Thr His Ala Gln Lys Asp Met Val 
    210                 215                 220                 


Gly Arg Gly His Ala Trp Leu Lys Gly Asp Asn Ile Arg Asp His Val 
225                 230                 235                 240 


Thr Arg Val Glu Gly Trp Met Trp Lys Asn Lys Leu Leu Thr Val Ala 
                245                 250                 255     


Ile Val Ala Leu Ala Trp Leu Met Val Asp Ser Trp Met Ala Arg Val 
            260                 265                 270         


Thr Val Ile Leu Leu Ala Leu Ser Leu Gly Pro Val Tyr Ala Thr Arg 
        275                 280                 285             


Cys Thr His Leu Glu Asn Arg Asp Phe Val Thr Gly Thr Gln Gly Thr 
    290                 295                 300                 


Thr Arg Val Ser Leu Val Leu Glu Leu Gly Gly Cys Val Thr Ile Thr 
305                 310                 315                 320 


Ala Glu Gly Lys Pro Ser Ile Asp Val Trp Leu Glu Asp Ile Phe Gln 
                325                 330                 335     


Glu Ser Pro Ala Glu Thr Arg Glu Tyr Cys Leu His Ala Lys Leu Thr 
            340                 345                 350         


Asn Thr Lys Val Glu Ala Arg Cys Pro Thr Thr Gly Pro Ala Thr Leu 
        355                 360                 365             


Pro Glu Glu His Gln Ala Asn Met Val Cys Lys Arg Asp Gln Ser Asp 
    370                 375                 380                 


Arg Gly Trp Gly Asn His Cys Gly Phe Phe Gly Lys Gly Ser Ile Val 
385                 390                 395                 400 


Ala Cys Ala Lys Phe Glu Cys Glu Glu Ala Lys Lys Ala Val Gly His 
                405                 410                 415     


Val Tyr Asp Ser Thr Lys Ile Thr Tyr Val Val Lys Val Glu Pro His 
            420                 425                 430         


Thr Gly Asp Tyr Leu Ala Ala Asn Glu Thr Asn Ser Asn Arg Lys Ser 
        435                 440                 445             


Ala Gln Phe Thr Val Ala Ser Glu Lys Val Ile Leu Arg Leu Gly Asp 
    450                 455                 460                 


Tyr Gly Asp Val Ser Leu Thr Cys Lys Val Ala Ser Gly Ile Asp Val 
465                 470                 475                 480 


Ala Gln Thr Val Val Met Ser Leu Gly Ser Ser Lys Asp His Leu Pro 
                485                 490                 495     


Ser Ala Trp Gln Val His Arg Asp Trp Phe Glu Asp Leu Ala Leu Pro 
            500                 505                 510         


Trp Lys His Lys Asp Asn Gln Asp Trp Asn Ser Val Glu Lys Leu Val 
        515                 520                 525             


Glu Phe Gly Pro Pro His Ala Val Lys Met Asp Val Phe Asn Leu Gly 
    530                 535                 540                 


Asp Gln Thr Ala Val Leu Leu Lys Ser Leu Ala Gly Val Pro Leu Ala 
545                 550                 555                 560 


Ser Val Glu Gly Gln Lys Tyr His Leu Lys Ser Gly His Val Thr Cys 
                565                 570                 575     


Asp Val Gly Leu Glu Lys Leu Lys Leu Lys Gly 
            580                 585         


<210>  11
<211>  1821
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, consensus POWV capsid and envelope 
       immunogen operably linked to a sequence encoding an IgE leader

<400>  11
atggattgga cttggattct gtttctggtc gcagccgcaa ctcgcgtgca tagcgtgact       60

acttcaaaag gcaaaggagg aggccccccc aggagaaagc tgaaggtgac cgccaacaag      120

agccgccctg ccacatcccc tatgccaaag ggcttcgtgc tgagcaggat gctgggcatc      180

ctgtggcacg cagtgaccgg aacagccaga ccccctgtgc tgaagatgtt ttggaagacc      240

gtgcctctga gacaggcaga ggccgccctg aagaagatca agagagtgat cggcaatctg      300

atgcagtctc tgcacatgag gggccggcgc aggagcggag tggactggac ctggacattc      360

ctgaccatgg ccctgatgac aatggccatg gccaccacaa tccaccgcga tagggagggc      420

tacatggtca tgagagcctc tagaggcagg aagagacgga gcgccaccgg aagagacgca      480

gcatctcagg tgcgggtgca gaacggcacc tgcgtgatcc tggccacaga catgggcgag      540

tggtgcgagg attctatcac ctacagctgc gtgacaatcg accaggagga ggagcccgtg      600

gacgtggatt gcttctgtcg cggcgtggat agggtgaagc tggagtatgg ccgctgtggc      660

aggcaggcag gctttagagg caagcggagc gtggtcatcc ctacccacgc acagaaggac      720

atggtgggaa ggggacacgc atggctgaag ggcgacaaca tcagagatca cgtgacccgg      780

gtggagggct ggatgtggaa gaataagctg ctgacagtgg ccatcgtggc cctggcctgg      840

ctgatggtgg attcttggat ggcccgggtg acagtgatcc tgctggccct gagcctggga      900

cccgtgtacg ccacccgctg cacacacctg gagaacaggg acttcgtgac cggaacacag      960

ggaaccacac gcgtgtccct ggtgctggag ctgggcggct gcgtgaccat cacagcagag     1020

ggcaagccaa gcatcgacgt gtggctggag gatatctttc aggagtcccc cgccgagaca     1080

agggagtatt gcctgcacgc caagctgacc aatacaaagg tggaggccag atgtcccacc     1140

acaggccctg ccacactgcc agaggagcac caggccaaca tggtgtgcaa gcgcgaccag     1200

tccgataggg gctggggcaa tcactgtggc ttctttggca agggctctat cgtggcctgc     1260

gccaagttcg agtgtgagga ggccaagaag gccgtgggcc acgtgtacga cagcaccaag     1320

atcacatatg tggtgaaggt ggagccacac accggcgatt acctggccgc caacgagaca     1380

aactccaatc ggaagtctgc ccagtttacc gtggcctccg agaaagtgat cctgcgcctg     1440

ggcgactatg gcgacgtgag cctgacctgc aaggtggcct ctggcatcga cgtggcccag     1500

acagtggtca tgtccctggg cagctccaag gatcacctgc cttctgcctg gcaggtgcac     1560

cgcgactggt ttgaggatct ggccctgcca tggaagcaca aggacaacca ggattggaat     1620

tctgtggaga agctggtgga gttcggccca ccccacgcag tgaagatgga cgtgtttaat     1680

ctgggcgatc agaccgccgt gctgctgaag agcctggcag gagtgccact ggcatccgtg     1740

gagggccaga agtatcacct gaaaagcggg catgtgacct gcgatgtggg gctggaaaaa     1800

ctgaaactga aagggtaatg a                                               1821


<210>  12
<211>  605
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, consensus POWV capsid and envelope 
       immunogen operably linked to an IgE leader

<400>  12

Met Asp Trp Thr Trp Ile Leu Phe Leu Val Ala Ala Ala Thr Arg Val 
1               5                   10                  15      


His Ser Val Thr Thr Ser Lys Gly Lys Gly Gly Gly Pro Pro Arg Arg 
            20                  25                  30          


Lys Leu Lys Val Thr Ala Asn Lys Ser Arg Pro Ala Thr Ser Pro Met 
        35                  40                  45              


Pro Lys Gly Phe Val Leu Ser Arg Met Leu Gly Ile Leu Trp His Ala 
    50                  55                  60                  


Val Thr Gly Thr Ala Arg Pro Pro Val Leu Lys Met Phe Trp Lys Thr 
65                  70                  75                  80  


Val Pro Leu Arg Gln Ala Glu Ala Ala Leu Lys Lys Ile Lys Arg Val 
                85                  90                  95      


Ile Gly Asn Leu Met Gln Ser Leu His Met Arg Gly Arg Arg Arg Ser 
            100                 105                 110         


Gly Val Asp Trp Thr Trp Thr Phe Leu Thr Met Ala Leu Met Thr Met 
        115                 120                 125             


Ala Met Ala Thr Thr Ile His Arg Asp Arg Glu Gly Tyr Met Val Met 
    130                 135                 140                 


Arg Ala Ser Arg Gly Arg Lys Arg Arg Ser Ala Thr Gly Arg Asp Ala 
145                 150                 155                 160 


Ala Ser Gln Val Arg Val Gln Asn Gly Thr Cys Val Ile Leu Ala Thr 
                165                 170                 175     


Asp Met Gly Glu Trp Cys Glu Asp Ser Ile Thr Tyr Ser Cys Val Thr 
            180                 185                 190         


Ile Asp Gln Glu Glu Glu Pro Val Asp Val Asp Cys Phe Cys Arg Gly 
        195                 200                 205             


Val Asp Arg Val Lys Leu Glu Tyr Gly Arg Cys Gly Arg Gln Ala Gly 
    210                 215                 220                 


Phe Arg Gly Lys Arg Ser Val Val Ile Pro Thr His Ala Gln Lys Asp 
225                 230                 235                 240 


Met Val Gly Arg Gly His Ala Trp Leu Lys Gly Asp Asn Ile Arg Asp 
                245                 250                 255     


His Val Thr Arg Val Glu Gly Trp Met Trp Lys Asn Lys Leu Leu Thr 
            260                 265                 270         


Val Ala Ile Val Ala Leu Ala Trp Leu Met Val Asp Ser Trp Met Ala 
        275                 280                 285             


Arg Val Thr Val Ile Leu Leu Ala Leu Ser Leu Gly Pro Val Tyr Ala 
    290                 295                 300                 


Thr Arg Cys Thr His Leu Glu Asn Arg Asp Phe Val Thr Gly Thr Gln 
305                 310                 315                 320 


Gly Thr Thr Arg Val Ser Leu Val Leu Glu Leu Gly Gly Cys Val Thr 
                325                 330                 335     


Ile Thr Ala Glu Gly Lys Pro Ser Ile Asp Val Trp Leu Glu Asp Ile 
            340                 345                 350         


Phe Gln Glu Ser Pro Ala Glu Thr Arg Glu Tyr Cys Leu His Ala Lys 
        355                 360                 365             


Leu Thr Asn Thr Lys Val Glu Ala Arg Cys Pro Thr Thr Gly Pro Ala 
    370                 375                 380                 


Thr Leu Pro Glu Glu His Gln Ala Asn Met Val Cys Lys Arg Asp Gln 
385                 390                 395                 400 


Ser Asp Arg Gly Trp Gly Asn His Cys Gly Phe Phe Gly Lys Gly Ser 
                405                 410                 415     


Ile Val Ala Cys Ala Lys Phe Glu Cys Glu Glu Ala Lys Lys Ala Val 
            420                 425                 430         


Gly His Val Tyr Asp Ser Thr Lys Ile Thr Tyr Val Val Lys Val Glu 
        435                 440                 445             


Pro His Thr Gly Asp Tyr Leu Ala Ala Asn Glu Thr Asn Ser Asn Arg 
    450                 455                 460                 


Lys Ser Ala Gln Phe Thr Val Ala Ser Glu Lys Val Ile Leu Arg Leu 
465                 470                 475                 480 


Gly Asp Tyr Gly Asp Val Ser Leu Thr Cys Lys Val Ala Ser Gly Ile 
                485                 490                 495     


Asp Val Ala Gln Thr Val Val Met Ser Leu Gly Ser Ser Lys Asp His 
            500                 505                 510         


Leu Pro Ser Ala Trp Gln Val His Arg Asp Trp Phe Glu Asp Leu Ala 
        515                 520                 525             


Leu Pro Trp Lys His Lys Asp Asn Gln Asp Trp Asn Ser Val Glu Lys 
    530                 535                 540                 


Leu Val Glu Phe Gly Pro Pro His Ala Val Lys Met Asp Val Phe Asn 
545                 550                 555                 560 


Leu Gly Asp Gln Thr Ala Val Leu Leu Lys Ser Leu Ala Gly Val Pro 
                565                 570                 575     


Leu Ala Ser Val Glu Gly Gln Lys Tyr His Leu Lys Ser Gly His Val 
            580                 585                 590         


Thr Cys Asp Val Gly Leu Glu Lys Leu Lys Leu Lys Gly 
        595                 600                 605 


<210>  13
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, IgE leader sequence

<400>  13

Met Asp Trp Thr Trp Ile Leu Phe Leu Val Ala Ala Ala Thr Arg Val 
1               5                   10                  15      


His Ser 
        


