                         SEQUENCE LISTING

<110>  Syngenta Crop Protection AG
       REYNOLDS, Clarence Michael
 
<120>  Insecticidal Proreins

<130>  81720-WO-REG-ORG-P-1

<150>  US 62/780,808
<151>  2018-12-17

<160>  40    

<170>  PatentIn version 3.5

<210>  1
<211>  118
<212>  PRT
<213>  Streptomyces species

<400>  1

Met Ala Ala Arg Ser Tyr Glu Ile Ala Ile Val Asn Leu Thr Asp Val 
1               5                   10                  15      


Glu Phe Thr Arg Lys Glu Ala His Leu Asp His Gly Val Trp Ser Lys 
            20                  25                  30          


Asp Gly Asn Tyr Thr Pro Pro Asp Lys Ile Ser Thr Gly Gln Thr Ala 
        35                  40                  45              


His Phe Gly Ser Glu Ser Gln Gly Val Ala Thr Gly Thr Glu Gly His 
    50                  55                  60                  


Val Ile Tyr Ser Ser Ser Ala Gly Asp Phe Arg Val Asp Trp Asp Asn 
65                  70                  75                  80  


Pro Tyr Ile Gly Gly Asp Ser Ser Ser Ala Lys Cys Pro Pro Ser Tyr 
                85                  90                  95      


Glu Lys Val Leu Ser Asp Ser Lys Gly Asn Asp Ala Thr Leu Lys Val 
            100                 105                 110         


Val Phe Tyr Lys Lys Ser 
        115             


<210>  2
<211>  118
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SMIP1Aa-A71L

<400>  2

Met Ala Ala Arg Ser Tyr Glu Ile Ala Ile Val Asn Leu Thr Asp Val 
1               5                   10                  15      


Glu Phe Thr Arg Lys Glu Ala His Leu Asp His Gly Val Trp Ser Lys 
            20                  25                  30          


Asp Gly Asn Tyr Thr Pro Pro Asp Lys Ile Ser Thr Gly Gln Thr Ala 
        35                  40                  45              


His Phe Gly Ser Glu Ser Gln Gly Val Ala Thr Gly Thr Glu Gly His 
    50                  55                  60                  


Val Ile Tyr Ser Ser Ser Leu Gly Asp Phe Arg Val Asp Trp Asp Asn 
65                  70                  75                  80  


Pro Tyr Ile Gly Gly Asp Ser Ser Ser Ala Lys Cys Pro Pro Ser Tyr 
                85                  90                  95      


Glu Lys Val Leu Ser Asp Ser Lys Gly Asn Asp Ala Thr Leu Lys Val 
            100                 105                 110         


Val Phe Tyr Lys Lys Ser 
        115             


<210>  3
<211>  118
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SMIP1Aa-A90L

<400>  3

Met Ala Ala Arg Ser Tyr Glu Ile Ala Ile Val Asn Leu Thr Asp Val 
1               5                   10                  15      


Glu Phe Thr Arg Lys Glu Ala His Leu Asp His Gly Val Trp Ser Lys 
            20                  25                  30          


Asp Gly Asn Tyr Thr Pro Pro Asp Lys Ile Ser Thr Gly Gln Thr Ala 
        35                  40                  45              


His Phe Gly Ser Glu Ser Gln Gly Val Ala Thr Gly Thr Glu Gly His 
    50                  55                  60                  


Val Ile Tyr Ser Ser Ser Ala Gly Asp Phe Arg Val Asp Trp Asp Asn 
65                  70                  75                  80  


Pro Tyr Ile Gly Gly Asp Ser Ser Ser Leu Lys Cys Pro Pro Ser Tyr 
                85                  90                  95      


Glu Lys Val Leu Ser Asp Ser Lys Gly Asn Asp Ala Thr Leu Lys Val 
            100                 105                 110         


Val Phe Tyr Lys Lys Ser 
        115             


<210>  4
<211>  118
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SMIP1Aa-C92L

<400>  4

Met Ala Ala Arg Ser Tyr Glu Ile Ala Ile Val Asn Leu Thr Asp Val 
1               5                   10                  15      


Glu Phe Thr Arg Lys Glu Ala His Leu Asp His Gly Val Trp Ser Lys 
            20                  25                  30          


Asp Gly Asn Tyr Thr Pro Pro Asp Lys Ile Ser Thr Gly Gln Thr Ala 
        35                  40                  45              


His Phe Gly Ser Glu Ser Gln Gly Val Ala Thr Gly Thr Glu Gly His 
    50                  55                  60                  


Val Ile Tyr Ser Ser Ser Ala Gly Asp Phe Arg Val Asp Trp Asp Asn 
65                  70                  75                  80  


Pro Tyr Ile Gly Gly Asp Ser Ser Ser Ala Lys Leu Pro Pro Ser Tyr 
                85                  90                  95      


Glu Lys Val Leu Ser Asp Ser Lys Gly Asn Asp Ala Thr Leu Lys Val 
            100                 105                 110         


Val Phe Tyr Lys Lys Ser 
        115             


<210>  5
<211>  118
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SMIP1Aa-C92S

<400>  5

Met Ala Ala Arg Ser Tyr Glu Ile Ala Ile Val Asn Leu Thr Asp Val 
1               5                   10                  15      


Glu Phe Thr Arg Lys Glu Ala His Leu Asp His Gly Val Trp Ser Lys 
            20                  25                  30          


Asp Gly Asn Tyr Thr Pro Pro Asp Lys Ile Ser Thr Gly Gln Thr Ala 
        35                  40                  45              


His Phe Gly Ser Glu Ser Gln Gly Val Ala Thr Gly Thr Glu Gly His 
    50                  55                  60                  


Val Ile Tyr Ser Ser Ser Ala Gly Asp Phe Arg Val Asp Trp Asp Asn 
65                  70                  75                  80  


Pro Tyr Ile Gly Gly Asp Ser Ser Ser Ala Lys Ser Pro Pro Ser Tyr 
                85                  90                  95      


Glu Lys Val Leu Ser Asp Ser Lys Gly Asn Asp Ala Thr Leu Lys Val 
            100                 105                 110         


Val Phe Tyr Lys Lys Ser 
        115             


<210>  6
<211>  118
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SMIP1Aa-G84L

<400>  6

Met Ala Ala Arg Ser Tyr Glu Ile Ala Ile Val Asn Leu Thr Asp Val 
1               5                   10                  15      


Glu Phe Thr Arg Lys Glu Ala His Leu Asp His Gly Val Trp Ser Lys 
            20                  25                  30          


Asp Gly Asn Tyr Thr Pro Pro Asp Lys Ile Ser Thr Gly Gln Thr Ala 
        35                  40                  45              


His Phe Gly Ser Glu Ser Gln Gly Val Ala Thr Gly Thr Glu Gly His 
    50                  55                  60                  


Val Ile Tyr Ser Ser Ser Ala Gly Asp Phe Arg Val Asp Trp Asp Asn 
65                  70                  75                  80  


Pro Tyr Ile Leu Gly Asp Ser Ser Ser Ala Lys Cys Pro Pro Ser Tyr 
                85                  90                  95      


Glu Lys Val Leu Ser Asp Ser Lys Gly Asn Asp Ala Thr Leu Lys Val 
            100                 105                 110         


Val Phe Tyr Lys Lys Ser 
        115             


<210>  7
<211>  118
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SMIP1Aa-G85L

<400>  7

Met Ala Ala Arg Ser Tyr Glu Ile Ala Ile Val Asn Leu Thr Asp Val 
1               5                   10                  15      


Glu Phe Thr Arg Lys Glu Ala His Leu Asp His Gly Val Trp Ser Lys 
            20                  25                  30          


Asp Gly Asn Tyr Thr Pro Pro Asp Lys Ile Ser Thr Gly Gln Thr Ala 
        35                  40                  45              


His Phe Gly Ser Glu Ser Gln Gly Val Ala Thr Gly Thr Glu Gly His 
    50                  55                  60                  


Val Ile Tyr Ser Ser Ser Ala Gly Asp Phe Arg Val Asp Trp Asp Asn 
65                  70                  75                  80  


Pro Tyr Ile Gly Leu Asp Ser Ser Ser Ala Lys Cys Pro Pro Ser Tyr 
                85                  90                  95      


Glu Lys Val Leu Ser Asp Ser Lys Gly Asn Asp Ala Thr Leu Lys Val 
            100                 105                 110         


Val Phe Tyr Lys Lys Ser 
        115             


<210>  8
<211>  118
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SMIP1Aa-V76L

<400>  8

Met Ala Ala Arg Ser Tyr Glu Ile Ala Ile Val Asn Leu Thr Asp Val 
1               5                   10                  15      


Glu Phe Thr Arg Lys Glu Ala His Leu Asp His Gly Val Trp Ser Lys 
            20                  25                  30          


Asp Gly Asn Tyr Thr Pro Pro Asp Lys Ile Ser Thr Gly Gln Thr Ala 
        35                  40                  45              


His Phe Gly Ser Glu Ser Gln Gly Val Ala Thr Gly Thr Glu Gly His 
    50                  55                  60                  


Val Ile Tyr Ser Ser Ser Ala Gly Asp Phe Arg Leu Asp Trp Asp Asn 
65                  70                  75                  80  


Pro Tyr Ile Gly Gly Asp Ser Ser Ser Ala Lys Cys Pro Pro Ser Tyr 
                85                  90                  95      


Glu Lys Val Leu Ser Asp Ser Lys Gly Asn Asp Ala Thr Leu Lys Val 
            100                 105                 110         


Val Phe Tyr Lys Lys Ser 
        115             


<210>  9
<211>  118
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SMIP1Aa-W78F

<400>  9

Met Ala Ala Arg Ser Tyr Glu Ile Ala Ile Val Asn Leu Thr Asp Val 
1               5                   10                  15      


Glu Phe Thr Arg Lys Glu Ala His Leu Asp His Gly Val Trp Ser Lys 
            20                  25                  30          


Asp Gly Asn Tyr Thr Pro Pro Asp Lys Ile Ser Thr Gly Gln Thr Ala 
        35                  40                  45              


His Phe Gly Ser Glu Ser Gln Gly Val Ala Thr Gly Thr Glu Gly His 
    50                  55                  60                  


Val Ile Tyr Ser Ser Ser Ala Gly Asp Phe Arg Val Asp Phe Asp Asn 
65                  70                  75                  80  


Pro Tyr Ile Gly Gly Asp Ser Ser Ser Ala Lys Cys Pro Pro Ser Tyr 
                85                  90                  95      


Glu Lys Val Leu Ser Asp Ser Lys Gly Asn Asp Ala Thr Leu Lys Val 
            100                 105                 110         


Val Phe Tyr Lys Lys Ser 
        115             


<210>  10
<211>  118
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SMIP1Aa-A71L/A90L

<400>  10

Met Ala Ala Arg Ser Tyr Glu Ile Ala Ile Val Asn Leu Thr Asp Val 
1               5                   10                  15      


Glu Phe Thr Arg Lys Glu Ala His Leu Asp His Gly Val Trp Ser Lys 
            20                  25                  30          


Asp Gly Asn Tyr Thr Pro Pro Asp Lys Ile Ser Thr Gly Gln Thr Ala 
        35                  40                  45              


His Phe Gly Ser Glu Ser Gln Gly Val Ala Thr Gly Thr Glu Gly His 
    50                  55                  60                  


Val Ile Tyr Ser Ser Ser Leu Gly Asp Phe Arg Val Asp Trp Asp Asn 
65                  70                  75                  80  


Pro Tyr Ile Gly Gly Asp Ser Ser Ser Leu Lys Cys Pro Pro Ser Tyr 
                85                  90                  95      


Glu Lys Val Leu Ser Asp Ser Lys Gly Asn Asp Ala Thr Leu Lys Val 
            100                 105                 110         


Val Phe Tyr Lys Lys Ser 
        115             


<210>  11
<211>  118
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SMIP1Aa-A71L/W78F

<400>  11

Met Ala Ala Arg Ser Tyr Glu Ile Ala Ile Val Asn Leu Thr Asp Val 
1               5                   10                  15      


Glu Phe Thr Arg Lys Glu Ala His Leu Asp His Gly Val Trp Ser Lys 
            20                  25                  30          


Asp Gly Asn Tyr Thr Pro Pro Asp Lys Ile Ser Thr Gly Gln Thr Ala 
        35                  40                  45              


His Phe Gly Ser Glu Ser Gln Gly Val Ala Thr Gly Thr Glu Gly His 
    50                  55                  60                  


Val Ile Tyr Ser Ser Ser Leu Gly Asp Phe Arg Val Asp Phe Asp Asn 
65                  70                  75                  80  


Pro Tyr Ile Gly Gly Asp Ser Ser Ser Ala Lys Cys Pro Pro Ser Tyr 
                85                  90                  95      


Glu Lys Val Leu Ser Asp Ser Lys Gly Asn Asp Ala Thr Leu Lys Val 
            100                 105                 110         


Val Phe Tyr Lys Lys Ser 
        115             


<210>  12
<211>  118
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SMIP1Aa-W78F/C92L

<400>  12

Met Ala Ala Arg Ser Tyr Glu Ile Ala Ile Val Asn Leu Thr Asp Val 
1               5                   10                  15      


Glu Phe Thr Arg Lys Glu Ala His Leu Asp His Gly Val Trp Ser Lys 
            20                  25                  30          


Asp Gly Asn Tyr Thr Pro Pro Asp Lys Ile Ser Thr Gly Gln Thr Ala 
        35                  40                  45              


His Phe Gly Ser Glu Ser Gln Gly Val Ala Thr Gly Thr Glu Gly His 
    50                  55                  60                  


Val Ile Tyr Ser Ser Ser Ala Gly Asp Phe Arg Val Asp Phe Asp Asn 
65                  70                  75                  80  


Pro Tyr Ile Gly Gly Asp Ser Ser Ser Ala Lys Leu Pro Pro Ser Tyr 
                85                  90                  95      


Glu Lys Val Leu Ser Asp Ser Lys Gly Asn Asp Ala Thr Leu Lys Val 
            100                 105                 110         


Val Phe Tyr Lys Lys Ser 
        115             


<210>  13
<211>  118
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SMIP1Aa-Y82F/I83L

<400>  13

Met Ala Ala Arg Ser Tyr Glu Ile Ala Ile Val Asn Leu Thr Asp Val 
1               5                   10                  15      


Glu Phe Thr Arg Lys Glu Ala His Leu Asp His Gly Val Trp Ser Lys 
            20                  25                  30          


Asp Gly Asn Tyr Thr Pro Pro Asp Lys Ile Ser Thr Gly Gln Thr Ala 
        35                  40                  45              


His Phe Gly Ser Glu Ser Gln Gly Val Ala Thr Gly Thr Glu Gly His 
    50                  55                  60                  


Val Ile Tyr Ser Ser Ser Ala Gly Asp Phe Arg Val Asp Trp Asp Asn 
65                  70                  75                  80  


Pro Phe Leu Gly Gly Asp Ser Ser Ser Ala Lys Cys Pro Pro Ser Tyr 
                85                  90                  95      


Glu Lys Val Leu Ser Asp Ser Lys Gly Asn Asp Ala Thr Leu Lys Val 
            100                 105                 110         


Val Phe Tyr Lys Lys Ser 
        115             


<210>  14
<211>  118
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SMIP1Aa-A2G/A3G

<400>  14

Met Gly Gly Arg Ser Tyr Glu Ile Ala Ile Val Asn Leu Thr Asp Val 
1               5                   10                  15      


Glu Phe Thr Arg Lys Glu Ala His Leu Asp His Gly Val Trp Ser Lys 
            20                  25                  30          


Asp Gly Asn Tyr Thr Pro Pro Asp Lys Ile Ser Thr Gly Gln Thr Ala 
        35                  40                  45              


His Phe Gly Ser Glu Ser Gln Gly Val Ala Thr Gly Thr Glu Gly His 
    50                  55                  60                  


Val Ile Tyr Ser Ser Ser Ala Gly Asp Phe Arg Val Asp Trp Asp Asn 
65                  70                  75                  80  


Pro Tyr Ile Gly Gly Asp Ser Ser Ser Ala Lys Cys Pro Pro Ser Tyr 
                85                  90                  95      


Glu Lys Val Leu Ser Asp Ser Lys Gly Asn Asp Ala Thr Leu Lys Val 
            100                 105                 110         


Val Phe Tyr Lys Lys Ser 
        115             


<210>  15
<211>  118
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SMIP1Aa-A2V/A3V

<400>  15

Met Val Val Arg Ser Tyr Glu Ile Ala Ile Val Asn Leu Thr Asp Val 
1               5                   10                  15      


Glu Phe Thr Arg Lys Glu Ala His Leu Asp His Gly Val Trp Ser Lys 
            20                  25                  30          


Asp Gly Asn Tyr Thr Pro Pro Asp Lys Ile Ser Thr Gly Gln Thr Ala 
        35                  40                  45              


His Phe Gly Ser Glu Ser Gln Gly Val Ala Thr Gly Thr Glu Gly His 
    50                  55                  60                  


Val Ile Tyr Ser Ser Ser Ala Gly Asp Phe Arg Val Asp Trp Asp Asn 
65                  70                  75                  80  


Pro Tyr Ile Gly Gly Asp Ser Ser Ser Ala Lys Cys Pro Pro Ser Tyr 
                85                  90                  95      


Glu Lys Val Leu Ser Asp Ser Lys Gly Asn Asp Ala Thr Leu Lys Val 
            100                 105                 110         


Val Phe Tyr Lys Lys Ser 
        115             


<210>  16
<211>  237
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SUMO-SMIP1Aa

<400>  16

Met Gly Ser Ser His His His His His His Gly Ser Gly Leu Val Pro 
1               5                   10                  15      


Arg Gly Ser Ala Ser Met Ser Asp Ser Glu Val Asn Gln Glu Ala Lys 
            20                  25                  30          


Pro Glu Val Lys Pro Glu Val Lys Pro Glu Thr His Ile Asn Leu Lys 
        35                  40                  45              


Val Ser Asp Gly Ser Ser Glu Ile Phe Phe Lys Ile Lys Lys Thr Thr 
    50                  55                  60                  


Pro Leu Arg Arg Leu Met Glu Ala Phe Ala Lys Arg Gln Gly Lys Glu 
65                  70                  75                  80  


Met Asp Ser Leu Arg Phe Leu Tyr Asp Gly Ile Arg Ile Gln Ala Asp 
                85                  90                  95      


Gln Thr Pro Glu Asp Leu Asp Met Glu Asp Asn Asp Ile Ile Glu Ala 
            100                 105                 110         


His Arg Glu Gln Ile Gly Gly Met Ala Ala Arg Ser Tyr Glu Ile Ala 
        115                 120                 125             


Ile Val Asn Leu Thr Asp Val Glu Phe Thr Arg Lys Glu Ala His Leu 
    130                 135                 140                 


Asp His Gly Val Trp Ser Lys Asp Gly Asn Tyr Thr Pro Pro Asp Lys 
145                 150                 155                 160 


Ile Ser Thr Gly Gln Thr Ala His Phe Gly Ser Glu Ser Gln Gly Val 
                165                 170                 175     


Ala Thr Gly Thr Glu Gly His Val Ile Tyr Ser Ser Ser Ala Gly Asp 
            180                 185                 190         


Phe Arg Val Asp Trp Asp Asn Pro Tyr Ile Gly Gly Asp Ser Ser Ser 
        195                 200                 205             


Ala Lys Cys Pro Pro Ser Tyr Glu Lys Val Leu Ser Asp Ser Lys Gly 
    210                 215                 220                 


Asn Asp Ala Thr Leu Lys Val Val Phe Tyr Lys Lys Ser 
225                 230                 235         


<210>  17
<211>  150
<212>  PRT
<213>  Streptomyces species

<400>  17

Met Ser Glu Asn Val Thr Gly Ala His Pro Tyr Gly Ser Ile His Trp 
1               5                   10                  15      


Val Pro Glu Ile Phe Val Asp Pro Ala Lys Ser Arg Gly Glu Gln Asp 
            20                  25                  30          


Met Ala Ala Arg Ser Tyr Asp Ile Ser Ile Val Asn Leu Thr Asn Met 
        35                  40                  45              


Glu Leu Thr Arg Glu Lys Ser His Leu Asp His Gly Val Trp Ser Gly 
    50                  55                  60                  


Glu Gly Ser Glu Thr Pro Pro Asp Lys Ile Ala Pro Gly Glu Thr Val 
65                  70                  75                  80  


His Phe Gly Ser Glu Ser Gln Gly Phe Met Thr Gly Thr Glu Gly Tyr 
                85                  90                  95      


Val Thr Tyr Gly Ser Pro Ala Gly Asp Phe Thr Val Tyr Trp Asp Asn 
            100                 105                 110         


Pro Tyr Val Gly Ser Asp Ser Ser Ser Ala Thr Cys Pro Ser Gly Tyr 
        115                 120                 125             


Glu Lys Val Lys Ser Asp Ser Ala Gly Asn Asn Ala Thr Leu Lys Val 
    130                 135                 140                 


Val Phe Tyr Glu Asn Ser 
145                 150 


<210>  18
<211>  357
<212>  DNA
<213>  Streptomyces species

<400>  18
atggccgcaa ggtcgtacga aatcgcgatc gtcaacctta ccgacgtgga gttcacgcgc       60

aaagaggcac atctcgatca tggggtgtgg agcaaggacg ggaactacac gcctccggac      120

aagatcagca ccggtcagac agcgcatttc ggaagtgaat cccagggagt ggcgaccgga      180

accgaggggc atgtgatcta ttcgtccagc gccggggatt ttcgcgtcga ctgggacaac      240

ccctacatcg ggggtgactc atcctccgcg aagtgcccgc cctcttacga aaaggtgctc      300

agtgactcca aaggtaacga cgcaaccctg aaggtggtct tctacaagaa gagctga         357


<210>  19
<211>  453
<212>  DNA
<213>  Streptomyces species

<400>  19
atgtctgaaa acgtgactgg tgcccatcca tatgggtcta tacattgggt cccggaaatc       60

ttcgtagatc cggcgaaaag ccgaggggag caggacatgg ccgcgagatc ctacgatatc      120

tcgatcgtca acctcaccaa tatggaactg acccgggaga agtcgcacct ggaccacggg      180

gtgtggagcg gggaaggcag cgaaacaccc ccggacaaga tagcccccgg agaaacggtg      240

catttcggca gcgaatccca gggattcatg acgggaaccg aagggtacgt cacctacggc      300

tcgcccgccg gagacttcac cgtctactgg gacaacccgt atgtcggttc ggattcgtcg      360

tccgcgacgt gcccgtcggg ttacgaaaag gtcaagagcg actccgcggg caacaacgcg      420

accctgaagg tggtcttcta cgagaacagc tga                                   453


<210>  20
<211>  357
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  E. coli optimized SMIP1Aa.

<400>  20
atggccgccc gtagttatga aattgcaatt gttaacttaa cggatgtaga atttacccgg       60

aaagaagccc atctcgacca cggagtttgg agcaaggatg gtaattacac tccgccggat      120

aaaatctcca ccgggcagac ggctcatttt ggtagcgaga gtcagggagt tgctaccggt      180

accgaaggtc atgtaattta ctcctccagc gcgggggact tccgcgtcga ttgggataat      240

ccatacattg gtggcgactc tagcagcgct aaatgcccac cgtcgtacga aaaagttctg      300

tccgattcca agggtaacga tgcaacctta aaagtggtct tttataaaaa gtcctaa         357


<210>  21
<211>  357
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  E. coli optimized SMIP1Aa-A71L.

<400>  21
atggccgccc gtagttatga aattgcaatt gttaacttaa cggatgtaga atttacccgg       60

aaagaagccc atctcgacca cggagtttgg agcaaggatg gtaattacac tccgccggat      120

aaaatctcca ccgggcagac ggctcatttt ggtagcgaga gtcagggagt tgctaccggt      180

accgaaggtc atgtaattta ctcctccagc ttaggggact tccgcgtcga ttgggataat      240

ccatacattg gtggcgactc tagcagcgct aaatgcccac cgtcgtacga aaaagttctg      300

tccgattcca agggtaacga tgcaacctta aaagtggtct tttataaaaa gtcctaa         357


<210>  22
<211>  357
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  E. coli optimized SMIP1Aa-A90L.

<400>  22
atggccgccc gtagttatga aattgcaatt gttaacttaa cggatgtaga atttacccgg       60

aaagaagccc atctcgacca cggagtttgg agcaaggatg gtaattacac tccgccggat      120

aaaatctcca ccgggcagac ggctcatttt ggtagcgaga gtcagggagt tgctaccggt      180

accgaaggtc atgtaattta ctcctccagc gcgggggact tccgcgtcga ttgggataat      240

ccatacattg gtggcgactc tagcagctta aaatgcccac cgtcgtacga aaaagttctg      300

tccgattcca agggtaacga tgcaacctta aaagtggtct tttataaaaa gtcctaa         357


<210>  23
<211>  357
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  E. coli optimized SMIP1Aa-C92L.

<400>  23
atggccgccc gtagttatga aattgcaatt gttaacttaa cggatgtaga atttacccgg       60

aaagaagccc atctcgacca cggagtttgg agcaaggatg gtaattacac tccgccggat      120

aaaatctcca ccgggcagac ggctcatttt ggtagcgaga gtcagggagt tgctaccggt      180

accgaaggtc atgtaattta ctcctccagc gcgggggact tccgcgtcga ttgggataat      240

ccatacattg gtggcgactc tagcagcgct aaactgccac cgtcgtacga aaaagttctg      300

tccgattcca agggtaacga tgcaacctta aaagtggtct tttataaaaa gtcctaa         357


<210>  24
<211>  357
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  E. coli optimized SMIP1Aa-C92C.

<400>  24
atggccgccc gtagttatga aattgcaatt gttaacttaa cggatgtaga atttacccgg       60

aaagaagccc atctcgacca cggagtttgg agcaaggatg gtaattacac tccgccggat      120

aaaatctcca ccgggcagac ggctcatttt ggtagcgaga gtcagggagt tgctaccggt      180

accgaaggtc atgtaattta ctcctccagc gcgggggact tccgcgtcga ttgggataat      240

ccatacattg gtggcgactc tagcagcgct aaatccccac cgtcgtacga aaaagttctg      300

tccgattcca agggtaacga tgcaacctta aaagtggtct tttataaaaa gtcctaa         357


<210>  25
<211>  357
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  E. coli optimized SMIP1Aa-G84L.

<400>  25
atggccgccc gtagttatga aattgcaatt gttaacttaa cggatgtaga atttacccgg       60

aaagaagccc atctcgacca cggagtttgg agcaaggatg gtaattacac tccgccggat      120

aaaatctcca ccgggcagac ggctcatttt ggtagcgaga gtcagggagt tgctaccggt      180

accgaaggtc atgtaattta ctcctccagc gcgggggact tccgcgtcga ttgggataat      240

ccatacattt taggcgactc tagcagcgct aaatgcccac cgtcgtacga aaaagttctg      300

tccgattcca agggtaacga tgcaacctta aaagtggtct tttataaaaa gtcctaa         357


<210>  26
<211>  357
<212>  DNA
<213>  Artificial Sequene

<220>
<223>  E. coli opti mized SMIP1Aa-G85L.

<400>  26
atggccgccc gtagttatga aattgcaatt gttaacttaa cggatgtaga atttacccgg       60

aaagaagccc atctcgacca cggagtttgg agcaaggatg gtaattacac tccgccggat      120

aaaatctcca ccgggcagac ggctcatttt ggtagcgaga gtcagggagt tgctaccggt      180

accgaaggtc atgtaattta ctcctccagc gcgggggact tccgcgtcga ttgggataat      240

ccatacattg gtttagactc tagcagcgct aaatgcccac cgtcgtacga aaaagttctg      300

tccgattcca agggtaacga tgcaacctta aaagtggtct tttataaaaa gtcctaa         357


<210>  27
<211>  357
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  E. coli optimized SMIP1Aa-V76L.

<400>  27
atggccgccc gtagttatga aattgcaatt gttaacttaa cggatgtaga atttacccgg       60

aaagaagccc atctcgacca cggagtttgg agcaaggatg gtaattacac tccgccggat      120

aaaatctcca ccgggcagac ggctcatttt ggtagcgaga gtcagggagt tgctaccggt      180

accgaaggtc atgtaattta ctcctccagc gcgggggact tccgcttaga ttgggataat      240

ccatacattg gtggcgactc tagcagcgct aaatgcccac cgtcgtacga aaaagttctg      300

tccgattcca agggtaacga tgcaacctta aaagtggtct tttataaaaa gtcctaa         357


<210>  28
<211>  357
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  E. coli optimized SMIP1Aa-W78F.

<400>  28
atggccgccc gtagttatga aattgcaatt gttaacttaa cggatgtaga atttacccgg       60

aaagaagccc atctcgacca cggagtttgg agcaaggatg gtaattacac tccgccggat      120

aaaatctcca ccgggcagac ggctcatttt ggtagcgaga gtcagggagt tgctaccggt      180

accgaaggtc atgtaattta ctcctccagc gcgggggact tccgcgtcga ttttgataat      240

ccatacattg gtggcgactc tagcagcgct aaatgcccac cgtcgtacga aaaagttctg      300

tccgattcca agggtaacga tgcaacctta aaagtggtct tttataaaaa gtcctaa         357


<210>  29
<211>  357
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  E. coli optimized SMIP1Aa-A71L/A90L.

<400>  29
atggccgccc gtagttatga aattgcaatt gttaacttaa cggatgtaga atttacccgg       60

aaagaagccc atctcgacca cggagtttgg agcaaggatg gtaattacac tccgccggat      120

aaaatctcca ccgggcagac ggctcatttt ggtagcgaga gtcagggagt tgctaccggt      180

accgaaggtc atgtaattta ctcctccagc ttaggggact tccgcgtcga ttgggataat      240

ccatacattg gtggcgactc tagcagctta aaatgcccac cgtcgtacga aaaagttctg      300

tccgattcca agggtaacga tgcaacctta aaagtggtct tttataaaaa gtcctaa         357


<210>  30
<211>  357
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  E. coli optimized SMIP1Aa-A71L/W78F.

<400>  30
atggccgccc gtagttatga aattgcaatt gttaacttaa cggatgtaga atttacccgg       60

aaagaagccc atctcgacca cggagtttgg agcaaggatg gtaattacac tccgccggat      120

aaaatctcca ccgggcagac ggctcatttt ggtagcgaga gtcagggagt tgctaccggt      180

accgaaggtc atgtaattta ctcctccagc ttaggggact tccgcgtcga ttttgataat      240

ccatacattg gtggcgactc tagcagcgct aaatgcccac cgtcgtacga aaaagttctg      300

tccgattcca agggtaacga tgcaacctta aaagtggtct tttataaaaa gtcctaa         357


<210>  31
<211>  357
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  E. coli optimized SMIP1Aa-W78F/C92L.

<400>  31
atggccgccc gtagttatga aattgcaatt gttaacttaa cggatgtaga atttacccgg       60

aaagaagccc atctcgacca cggagtttgg agcaaggatg gtaattacac tccgccggat      120

aaaatctcca ccgggcagac ggctcatttt ggtagcgaga gtcagggagt tgctaccggt      180

accgaaggtc atgtaattta ctcctccagc gcgggggact tccgcgtcga ttttgataat      240

ccatacattg gtggcgactc tagcagcgct aaactgccac cgtcgtacga aaaagttctg      300

tccgattcca agggtaacga tgcaacctta aaagtggtct tttataaaaa gtcctaa         357


<210>  32
<211>  357
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  E. coli optimized SMIP1Aa-Y82F/I83L.

<400>  32
atggccgccc gtagttatga aattgcaatt gttaacttaa cggatgtaga atttacccgg       60

aaagaagccc atctcgacca cggagtttgg agcaaggatg gtaattacac tccgccggat      120

aaaatctcca ccgggcagac ggctcatttt ggtagcgaga gtcagggagt tgctaccggt      180

accgaaggtc atgtaattta ctcctccagc gcgggggact tccgcgtcga ttgggataat      240

ccattcctgg gtggcgactc tagcagcgct aaatgcccac cgtcgtacga aaaagttctg      300

tccgattcca agggtaacga tgcaacctta aaagtggtct tttataaaaa gtcctaa         357


<210>  33
<211>  357
<212>  DNA
<213>  artificial Sequence

<220>
<223>  E. coli optimized SMIP1Aa-A2G/A3G.

<400>  33
atgggcggtc gtagttatga aattgcaatt gttaacttaa cggatgtaga atttacccgg       60

aaagaagccc atctcgacca cggagtttgg agcaaggatg gtaattacac tccgccggat      120

aaaatctcca ccgggcagac ggctcatttt ggtagcgaga gtcagggagt tgctaccggt      180

accgaaggtc atgtaattta ctcctccagc gcgggggact tccgcgtcga ttgggataat      240

ccatacattg gtggcgactc tagcagcgct aaatgcccac cgtcgtacga aaaagttctg      300

tccgattcca agggtaacga tgcaacctta aaagtggtct tttataaaaa gtcctaa         357


<210>  34
<211>  357
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  E. coli optimized SMIP1Aa-A2V/A3V.

<400>  34
atggtggtcc gtagttatga aattgcaatt gttaacttaa cggatgtaga atttacccgg       60

aaagaagccc atctcgacca cggagtttgg agcaaggatg gtaattacac tccgccggat      120

aaaatctcca ccgggcagac ggctcatttt ggtagcgaga gtcagggagt tgctaccggt      180

accgaaggtc atgtaattta ctcctccagc gcgggggact tccgcgtcga ttgggataat      240

ccatacattg gtggcgactc tagcagcgct aaatgcccac cgtcgtacga aaaagttctg      300

tccgattcca agggtaacga tgcaacctta aaagtggtct tttataaaaa gtcctaa         357


<210>  35
<211>  714
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  E. coli optimized SUMO-SMIP1Aa.

<400>  35
atgggcagca gccatcatca tcatcatcac ggcagcggcc tggtgccgcg cggcagcgct       60

agcatgtcgg actcagaagt caatcaagaa gctaagccag aggtcaagcc agaagtcaag      120

cctgagactc acatcaattt aaaggtgtcc gatggatctt cagagatctt cttcaagatc      180

aaaaagacca ctcctttaag aaggctgatg gaagcgttcg ctaaaagaca gggtaaggaa      240

atggactcct taagattctt gtacgacggt attagaattc aagctgatca gacccctgaa      300

gatttggaca tggaggataa cgatattatt gaggctcaca gagaacagat tggtggtatg      360

gccgcccgta gttatgaaat tgcaattgtt aacttaacgg atgtagaatt tacccggaaa      420

gaagcccatc tcgaccacgg agtttggagc aaggatggta attacactcc gccggataaa      480

atctccaccg ggcagacggc tcattttggt agcgagagtc agggagttgc taccggtacc      540

gaaggtcatg taatttactc ctccagcgcg ggggacttcc gcgtcgattg ggataatcca      600

tacattggtg gcgactctag cagcgctaaa tgcccaccgt cgtacgaaaa agttctgtcc      660

gattccaagg gtaacgatgc aaccttaaaa gtggtctttt ataaaaagtc ctaa            714


<210>  36
<211>  453
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  E. coli optimized SMIP2Aa.

<400>  36
atgtctgaga atgtgacggg ggcgcatccc tatggctcta tccactgggt ccccgaaatt       60

tttgtagatc cagcgaagtc ccgtggtgag caggacatgg ctgcgcgtag ttacgatatt      120

tcgattgtta atctgactaa catggagctt acccgtgaga aaagtcactt agatcatggc      180

gtctggtctg gagagggaag tgaaacacct cccgacaaga tcgctcccgg tgagactgtc      240

cactttgggt cagagtcgca aggatttatg acgggaacgg agggatatgt cacatacgga      300

tccccggcgg gtgattttac cgtatattgg gataatcctt acgttggttc ggattcttca      360

agtgcgacat gcccctcggg atacgaaaag gttaaatcgg acagtgcagg gaacaacgca      420

acgctgaaag ttgtgttcta tgaaaatagc taa                                   453


<210>  37
<211>  354
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Maize optimized SMIP1Aa.

<400>  37
atggccgcca ggtcctacga gatcgccatc gtgaacctga ccgacgtgga gttcaccagg       60

aaggaggccc acctggacca cggcgtgtgg tccaaggacg gcaactacac cccgccggac      120

aagatctcca ccggccagac cgcccacttc ggctccgagt cccagggcgt ggccaccggc      180

accgagggcc acgtgatcta ctcctcctcc gccggcgact tcagggtgga ctgggacaac      240

ccgtacatcg gcggcgactc ctcctccgcc aagtgcccgc cgtcctacga gaaggtgctg      300

tccgactcca agggcaacga cgccaccctg aaggtggtgt tctacaagaa gtcc            354


<210>  38
<211>  354
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Maize optimized SMIP1Aa-W78F/A92L.

<400>  38
atggccgcca ggtcctacga gatcgccatc gtgaacctga ccgacgtgga gttcaccagg       60

aaggaggccc acctggacca cggcgtgtgg tccaaggacg gcaactacac cccgccggac      120

aagatctcca ccggccagac cgcccacttc ggctccgagt cccagggcgt ggccaccggc      180

accgagggcc acgtgatcta ctcctcctcc gccggcgact tcagggtgga cttcgacaac      240

ccgtacatcg gcggcgactc ctcctccgcc aagctgccgc cgtcctacga gaaggtgctg      300

tccgactcca agggcaacga cgccaccctg aaggtggtgt tctacaagaa gtcc            354


<210>  39
<211>  354
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Maize optimized SMIP1Aa-A71L/A90L.

<400>  39
atggccgcca ggtcctacga gatcgccatc gtgaacctga ccgacgtgga gttcaccagg       60

aaggaggccc acctggacca cggcgtgtgg tccaaggacg gcaactacac cccgccggac      120

aagatctcca ccggccagac cgcccacttc ggctccgagt cccagggcgt ggccaccggc      180

accgagggcc acgtgatcta ctcctcctcc ctgggcgact tcagggtgga ctgggacaac      240

ccgtacatcg gcggcgactc ctcctccctg aagtgcccgc cgtcctacga gaaggtgctg      300

tccgactcca agggcaacga cgccaccctg aaggtggtgt tctacaagaa gtcc            354


<210>  40
<211>  453
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Maize optimized SMIP2Aa.

<400>  40
atgtccgaga acgtgaccgg cgcccacccg tacggctcca tccactgggt gccggagatc       60

ttcgtggacc cggccaagtc caggggcgag caggacatgg ccgccaggtc ctacgacatc      120

tccatcgtga acctgaccaa catggagctg accagggaga agtcccacct ggaccacggc      180

gtgtggtccg gcgagggctc cgagaccccg ccggacaaga tcgccccggg cgagaccgtg      240

cacttcggct ccgagtccca gggcttcatg accggcaccg agggctacgt gacctacggc      300

tccccggccg gcgacttcac cgtgtactgg gacaacccgt acgtgggctc cgactcctcc      360

tccgccacct gcccgtccgg ctacgagaag gtgaagtccg actccgccgg caacaacgcc      420

accctgaagg tggtgttcta cgagaactcc tga                                   453


