                         SEQUENCE LISTING

<110>  BioPharma Translationsinstitut Dessau Forschungs GmbH
 
<120>  Trypsin variants with improved enzymatic properties

<130>  P47121-WO

<150>  EP18214031.9
<151>  2018-12-19

<160>  64    

<170>  PatentIn version 3.5

<210>  1
<211>  223
<212>  PRT
<213>  artificial

<220>
<223>  Rat anionic trypsin II

<400>  1

Ile Val Gly Gly Tyr Thr Cys Gln Glu Asn Ser Val Pro Tyr Gln Val 
1               5                   10                  15      


Ser Leu Asn Ser Gly Tyr His Phe Cys Gly Gly Ser Leu Ile Asn Asp 
            20                  25                  30          


Gln Trp Val Val Ser Ala Ala His Cys Tyr Lys Ser Arg Ile Gln Val 
        35                  40                  45              


Arg Leu Gly Glu His Asn Ile Asn Val Leu Glu Gly Asn Glu Gln Phe 
    50                  55                  60                  


Val Asn Ala Ala Lys Ile Ile Lys His Pro Asn Phe Asp Arg Lys Thr 
65                  70                  75                  80  


Leu Asn Asn Asp Ile Met Leu Ile Lys Leu Ser Ser Pro Val Lys Leu 
                85                  90                  95      


Asn Ala Arg Val Ala Thr Val Ala Leu Pro Ser Ser Cys Ala Pro Ala 
            100                 105                 110         


Gly Thr Gln Cys Leu Ile Ser Gly Trp Gly Asn Thr Leu Ser Ser Gly 
        115                 120                 125             


Val Asn Glu Pro Asp Leu Leu Gln Cys Leu Asp Ala Pro Leu Leu Pro 
    130                 135                 140                 


Gln Ala Asp Cys Glu Ala Ser Tyr Pro Gly Lys Ile Thr Asp Asn Met 
145                 150                 155                 160 


Val Cys Val Gly Phe Leu Glu Gly Gly Lys Asp Ser Cys Gln Gly Asp 
                165                 170                 175     


Ser Gly Gly Pro Val Val Cys Asn Gly Glu Leu Gln Gly Ile Val Ser 
            180                 185                 190         


Trp Gly Tyr Gly Cys Ala Leu Pro Asp Asn Pro Gly Val Tyr Thr Lys 
        195                 200                 205             


Val Cys Asn Tyr Val Asp Trp Ile Gln Asp Thr Ile Ala Ala Asn 
    210                 215                 220             


<210>  2
<211>  245
<212>  PRT
<213>  artificial

<220>
<223>  anti-Her2-Fab-LC_RRKH-HC_YRAH, Heavy chain

<400>  2

Glu Val Lys Leu Gln Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Asn Ile Lys Asp Thr 
            20                  25                  30          


Tyr Ile His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Arg Ile Tyr Pro Thr Asn Gly Tyr Thr Arg Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Ala Asp Thr Ser Lys Asn Thr Ala Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ser Arg Trp Gly Gly Asp Gly Phe Tyr Ala Met Asp Tyr Trp Gly Gln 
            100                 105                 110         


Gly Thr Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val 
        115                 120                 125             


Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala 
    130                 135                 140                 


Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser 
145                 150                 155                 160 


Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val 
                165                 170                 175     


Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro 
            180                 185                 190         


Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys 
        195                 200                 205             


Pro Ser Asn Thr Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys Ala 
    210                 215                 220                 


Asp Lys Pro Gly Gly Tyr Arg Ala His Ala Gly Glu Gln Lys Leu Ile 
225                 230                 235                 240 


Ser Glu Glu Asp Leu 
                245 


<210>  3
<211>  225
<212>  PRT
<213>  artificial

<220>
<223>  anti-Her2-Fab- LC_RRKH-HC_YRAH, Light chain

<400>  3

Asp Ile Glu Leu Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Asp Val Asn Thr Ala 
            20                  25                  30          


Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Ser Ala Ser Phe Leu Tyr Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Arg Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln His Tyr Thr Thr Pro Pro 
                85                  90                  95      


Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys Arg Thr Val Ala Ala 
            100                 105                 110         


Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly 
        115                 120                 125             


Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala 
    130                 135                 140                 


Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln 
145                 150                 155                 160 


Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser 
                165                 170                 175     


Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val Tyr 
            180                 185                 190         


Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys Ser 
        195                 200                 205             


Phe Asn Arg Gly Glu Cys Leu Ser Pro Gly Gly Arg Arg Lys His Ala 
    210                 215                 220                 


Gly 
225 


<210>  4
<211>  9
<212>  PRT
<213>  artificial

<220>
<223>  Polypeptide

<400>  4

Pro Gly Gly Tyr Arg Ala His Ala Gly 
1               5                   


<210>  5
<211>  9
<212>  PRT
<213>  artificial

<220>
<223>  Polypeptide

<400>  5

Pro Gly Gly Phe Arg Ala His Ala Gly 
1               5                   


<210>  6
<211>  9
<212>  PRT
<213>  artificial

<220>
<223>  Polypeptide

<400>  6

Pro Gly Gly Trp Arg Ala His Ala Gly 
1               5                   


<210>  7
<211>  9
<212>  PRT
<213>  artificial

<220>
<223>  Polypeptide

<400>  7

Pro Gly Gly Leu Arg Ala His Ala Gly 
1               5                   


<210>  8
<211>  9
<212>  PRT
<213>  artificial

<220>
<223>  Polypeptide

<400>  8

Pro Gly Gly Asp Arg Ala His Ala Gly 
1               5                   


<210>  9
<211>  9
<212>  PRT
<213>  artificial

<220>
<223>  Polypeptide

<400>  9

Pro Gly Gly Arg Arg Ala His Ala Gly 
1               5                   


<210>  10
<211>  9
<212>  PRT
<213>  artificial

<220>
<223>  Polypeptide

<400>  10

Pro Gly Gly Ala Arg Ala His Ala Gly 
1               5                   


<210>  11
<211>  9
<212>  PRT
<213>  artificial

<220>
<223>  Polypeptide

<400>  11

Pro Gly Gly Tyr Ala Ala His Ala Gly 
1               5                   


<210>  12
<211>  9
<212>  PRT
<213>  artificial

<220>
<223>  Polypeptide

<400>  12

Pro Gly Gly Tyr Asp Ala His Ala Gly 
1               5                   


<210>  13
<211>  9
<212>  PRT
<213>  artificial

<220>
<223>  Polypeptide

<400>  13

Pro Gly Gly Tyr Glu Ala His Ala Gly 
1               5                   


<210>  14
<211>  9
<212>  PRT
<213>  artificial

<220>
<223>  Polypeptide

<400>  14

Pro Gly Gly Tyr Lys Ala His Ala Gly 
1               5                   


<210>  15
<211>  9
<212>  PRT
<213>  artificial

<220>
<223>  Polypeptide

<400>  15

Pro Gly Gly Tyr Arg Ala Ala Ala Gly 
1               5                   


<210>  16
<211>  9
<212>  PRT
<213>  artificial

<220>
<223>  Polypeptide

<400>  16

Pro Gly Gly Tyr Arg Ala Asn Ala Gly 
1               5                   


<210>  17
<211>  9
<212>  PRT
<213>  artificial

<220>
<223>  Polypeptide

<400>  17

Pro Gly Gly Tyr Arg Ala Lys Ala Gly 
1               5                   


<210>  18
<211>  9
<212>  PRT
<213>  artificial

<220>
<223>  Polypeptide

<400>  18

Pro Gly Gly Tyr Arg Ala Asp Ala Gly 
1               5                   


<210>  19
<211>  9
<212>  PRT
<213>  artificial

<220>
<223>  Polypeptide

<400>  19

Pro Gly Gly Tyr Arg Lys His Ala Gly 
1               5                   


<210>  20
<211>  9
<212>  PRT
<213>  artificial

<220>
<223>  Polypeptide

<400>  20

Pro Gly Gly Phe Arg Lys His Ala Gly 
1               5                   


<210>  21
<211>  9
<212>  PRT
<213>  artificial

<220>
<223>  Polypeptide

<400>  21

Pro Gly Gly Trp Arg Lys His Ala Gly 
1               5                   


<210>  22
<211>  9
<212>  PRT
<213>  artificial

<220>
<223>  Polypeptide

<400>  22

Pro Gly Gly Leu Arg Lys His Ala Gly 
1               5                   


<210>  23
<211>  9
<212>  PRT
<213>  artificial

<220>
<223>  Polypeptide

<400>  23

Pro Gly Gly Asp Arg Lys His Ala Gly 
1               5                   


<210>  24
<211>  9
<212>  PRT
<213>  artificial

<220>
<223>  Polypeptide

<400>  24

Pro Gly Gly Arg Arg Lys His Ala Gly 
1               5                   


<210>  25
<211>  9
<212>  PRT
<213>  artificial

<220>
<223>  Polypeptide

<400>  25

Pro Gly Gly Ala Arg Lys His Ala Gly 
1               5                   


<210>  26
<211>  9
<212>  PRT
<213>  artificial

<220>
<223>  Polypeptide

<400>  26

Pro Gly Gly Tyr Ala Lys His Ala Gly 
1               5                   


<210>  27
<211>  9
<212>  PRT
<213>  artificial

<220>
<223>  Polypeptide

<400>  27

Pro Gly Gly Tyr Asp Lys His Ala Gly 
1               5                   


<210>  28
<211>  9
<212>  PRT
<213>  artificial

<220>
<223>  Polypeptide

<400>  28

Pro Gly Gly Tyr Met Lys His Ala Gly 
1               5                   


<210>  29
<211>  9
<212>  PRT
<213>  artificial

<220>
<223>  Polypeptide

<400>  29

Pro Gly Gly Tyr Arg Arg His Ala Gly 
1               5                   


<210>  30
<211>  9
<212>  PRT
<213>  artificial

<220>
<223>  Polypeptide

<400>  30

Pro Gly Gly Tyr Arg Lys Ala Ala Gly 
1               5                   


<210>  31
<211>  9
<212>  PRT
<213>  artificial

<220>
<223>  Polypeptide

<400>  31

Pro Gly Gly Tyr Arg Lys Asn Ala Gly 
1               5                   


<210>  32
<211>  9
<212>  PRT
<213>  artificial

<220>
<223>  Polypeptide

<400>  32

Pro Gly Gly Tyr Arg Lys Lys Ala Gly 
1               5                   


<210>  33
<211>  9
<212>  PRT
<213>  artificial

<220>
<223>  Polypeptide

<400>  33

Pro Gly Gly Tyr Arg Lys Asp Ala Gly 
1               5                   


<210>  34
<211>  9
<212>  PRT
<213>  artificial

<220>
<223>  Polypeptide

<400>  34

Pro Gly Gly Tyr Lys Arg Lys Ala Gly 
1               5                   


<210>  35
<211>  9
<212>  PRT
<213>  artificial

<220>
<223>  Polypeptide

<400>  35

Pro Gly Gly Arg Met Lys His Ala Gly 
1               5                   


<210>  36
<211>  5
<212>  PRT
<213>  artificial

<220>
<223>  Polypeptide

<400>  36

Arg Ala His Ala Lys 
1               5   


<210>  37
<211>  5
<212>  PRT
<213>  artificial

<220>
<223>  Polypeptide

<400>  37

Ala Ala His Ala Lys 
1               5   


<210>  38
<211>  5
<212>  PRT
<213>  artificial

<220>
<223>  Polypeptide

<400>  38

Asp Ala His Ala Lys 
1               5   


<210>  39
<211>  5
<212>  PRT
<213>  artificial

<220>
<223>  Polypeptide

<400>  39

Glu Ala His Ala Lys 
1               5   


<210>  40
<211>  5
<212>  PRT
<213>  artificial

<220>
<223>  Polypeptide

<400>  40

Lys Ala His Ala Lys 
1               5   


<210>  41
<211>  5
<212>  PRT
<213>  artificial

<220>
<223>  Polypeptide

<400>  41

Arg Ala Ala Ala Lys 
1               5   


<210>  42
<211>  5
<212>  PRT
<213>  artificial

<220>
<223>  Polypeptide

<400>  42

Arg Ala Asn Ala Lys 
1               5   


<210>  43
<211>  5
<212>  PRT
<213>  artificial

<220>
<223>  Polypeptide

<400>  43

Arg Ala Lys Ala Lys 
1               5   


<210>  44
<211>  5
<212>  PRT
<213>  artificial

<220>
<223>  Polypeptide

<400>  44

Arg Ala Asp Ala Lys 
1               5   


<210>  45
<211>  5
<212>  PRT
<213>  artificial

<220>
<223>  Polypeptide

<400>  45

Arg Lys His Ala Lys 
1               5   


<210>  46
<211>  5
<212>  PRT
<213>  artificial

<220>
<223>  Polypeptide

<400>  46

Ala Lys His Ala Lys 
1               5   


<210>  47
<211>  5
<212>  PRT
<213>  artificial

<220>
<223>  Polypeptide

<400>  47

Asp Lys His Ala Lys 
1               5   


<210>  48
<211>  5
<212>  PRT
<213>  artificial

<220>
<223>  Polypeptide

<400>  48

Met Lys His Ala Lys 
1               5   


<210>  49
<211>  5
<212>  PRT
<213>  artificial

<220>
<223>  Polypeptide

<400>  49

Arg Arg His Ala Lys 
1               5   


<210>  50
<211>  5
<212>  PRT
<213>  artificial

<220>
<223>  Polypeptide

<400>  50

Arg Lys Ala Ala Lys 
1               5   


<210>  51
<211>  5
<212>  PRT
<213>  artificial

<220>
<223>  Polypeptide

<400>  51

Arg Lys Asn Ala Lys 
1               5   


<210>  52
<211>  5
<212>  PRT
<213>  artificial

<220>
<223>  Polypeptide

<400>  52

Arg Lys Lys Ala Lys 
1               5   


<210>  53
<211>  5
<212>  PRT
<213>  artificial

<220>
<223>  Polypeptide

<400>  53

Arg Lys Asp Ala Lys 
1               5   


<210>  54
<211>  5
<212>  PRT
<213>  artificial

<220>
<223>  Polypeptide

<400>  54

Lys Arg Lys Ala Lys 
1               5   


<210>  55
<211>  5
<212>  PRT
<213>  artificial

<220>
<223>  Polypeptide

<400>  55

Arg Ala His Ala Lys 
1               5   


<210>  56
<211>  5
<212>  PRT
<213>  artificial

<220>
<223>  Polypeptide

<400>  56

Arg Lys His Ala Lys 
1               5   


<210>  57
<211>  5
<212>  PRT
<213>  artificial

<220>
<223>  Polypeptide

<400>  57

Arg Lys Lys Ala Lys 
1               5   


<210>  58
<211>  5
<212>  PRT
<213>  artificial

<220>
<223>  Polypeptide

<400>  58

Arg Lys Ala Ala Lys 
1               5   


<210>  59
<211>  5
<212>  PRT
<213>  artificial

<220>
<223>  Polypeptide

<400>  59

Lys Arg Lys Ala Lys 
1               5   


<210>  60
<211>  5
<212>  PRT
<213>  artificial

<220>
<223>  Polypeptide

<400>  60

Arg Lys His Ala Lys 
1               5   


<210>  61
<211>  8
<212>  PRT
<213>  artificial

<220>
<223>  Polypeptide

<400>  61

Arg Lys Lys Ala Lys Ala Ala Lys 
1               5               


<210>  62
<211>  5
<212>  PRT
<213>  artificial

<220>
<223>  Polypeptide

<400>  62

Arg Lys Lys Ala Lys 
1               5   


<210>  63
<211>  5
<212>  PRT
<213>  artificial

<220>
<223>  Polypeptide

<400>  63

Arg Lys Lys Ala Lys 
1               5   


<210>  64
<211>  1552
<212>  DNA
<213>  artificial

<220>
<223>  Gene sequence of anti-Her2-Fab-LC_RRKH-HC_YRAH

<400>  64
atgaagaaaa ccgcgattgc gattgcggtg gcgctggcgg gctttgcgac cgtggcgcag       60

gcggatattg aactgaccca gagcccgagc agcctgagcg cgagcgtggg cgatcgcgtg      120

accattacct gccgcgcgag ccaggatgtg aacaccgcgg tggcgtggta tcagcagaaa      180

ccgggcaaag cgccgaaact gctgatttat agcgcgagct ttctgtatag cggcgtgccg      240

agccgcttta gcggcagccg cagcggcacc gattttaccc tgaccattag cagcctgcag      300

ccggaagatt ttgcgaccta ttattgccag cagcattata ccaccccgcc gacctttggc      360

cagggcacca aactggaaat taaacgcacc gtggcggcgc cgagcgtgtt tatttttccg      420

ccgagcgatg aacagctgaa aagcggcacc gcgagcgtgg tgtgcctgct gaacaacttt      480

tatccgcgcg aagcgaaagt gcagtggaaa gtggataacg cgctgcagag cggcaacagc      540

caggaaagcg tgaccgaaca ggatagcaaa gatagcacct atagcctgag cagcaccctg      600

accctgagca aagcggatta tgaaaaacat aaagtgtatg cgtgcgaagt gacccatcag      660

ggcctgagca gcccggtgac caaatctttt aaccgcggcg aatgcctgag ccccggagga      720

cgccgcaaac atgcgggctg aggaggaaaa aaaaatgaaa aagacagcta tcgcaattgc      780

agtggcgcta gctggtttcg ccaccgtggc gcaagctgaa gtgaaactgc aggaaagcgg      840

tggtggtctg gtgcagccgg gtggtagcct gcgcctgagc tgcgcggcga gcggctttaa      900

cattaaagat acctatattc attgggtgcg ccaggcgccg ggcaaaggcc tggaatgggt      960

ggcgcgcatt tatccgacca acggctatac ccgctatgcg gatagcgtga aaggccgctt     1020

taccattagc gcggatacca gcaaaaacac cgcgtatctg cagatgaaca gcctgcgcgc     1080

ggaagatacc gcggtgtatt attgcagccg ctggggcggc gatggctttt atgcgatgga     1140

ttattggggc cagggcaccc tggtgaccgt gagcagcgcg agcaccaaag gcccgagcgt     1200

gtttccgctg gcgccgagca gcaaaagcac cagcggcggc accgcggcgc tgggctgcct     1260

ggtgaaagat tattttccgg aaccggtgac cgtgagctgg aacagcggcg cgctgaccag     1320

cggcgtgcat acctttccgg cggtgctgca gagcagcggc ctgtatagcc tgagcagcgt     1380

ggtgaccgtg ccgagcagca gcctgggcac ccagacctat atttgcaacg tgaaccataa     1440

accgagcaac accaaagtgg ataaaaaagt ggaaccgaaa agctgcgcgg ataaacccgg     1500

aggatatcgc gcgcatgcgg gcgaacagaa actgattagc gaagaagatc tg             1552


